FastQCFastQC Report
Fri 27 May 2016
SRR1233086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50948328
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG14529692.8518482490730612TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2250180.4416592434593732TruSeq Adapter, Index 9 (100% over 50bp)
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1535810.3014446322949008No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1240780.24353694197776227No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT1166090.2288769908209745No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1098020.21551639535648745No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT962830.1889816678576773No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT816850.160329108346794No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC768280.15079592013304147No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG738170.14488601078331756No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT677450.13296805343641505No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT676420.13276588782265827No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA670060.13151756422703412No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660620.1296647065630888No Hit
ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT631420.12393340955173249No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG611620.12004711911252515No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT605060.11875954005791907No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT602030.1181648198543434No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA596270.11703426263566491No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC595290.11684191088665365No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT536140.10523210889275897No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA533030.10462168650559053No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT517400.10155387238615562No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG516590.10139488777727898No Hit
GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT512930.10067651287791035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG2072600.081.480131
GATCGGA2407500.071.82122
ATCGGAA2445800.070.6709443
TCGGAAG2462000.070.018814
CGGAAGA2486250.069.47575
GAAGAGC2700950.063.8511167
AGAGCAC2729050.063.2059029
GGAAGAG2792100.061.9302676
CGCGTGT488800.061.0900421
AAGAGCA2904100.059.4238368
GGATCGG65050.056.568471
GCGTGTT594550.050.544812
GTTTTTT6250200.041.5965961
CTCGTAT2280150.041.2447242-43
ATCTCGT2251050.040.50200340-41
ACGATCA2502400.040.3798632-33
TATGCCG2447150.040.23099546-47
CGTCTGA2492050.040.19316516-17
TGCCGTC2415800.040.05327648-49
AGTCACG2479600.039.93753428-29
Produced by FastQC (version 0.11.5)