##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50948328 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0708956925927 31.0 31.0 34.0 25.0 34.0 2 31.024907863512222 33.0 31.0 34.0 27.0 34.0 3 31.201151645251244 34.0 31.0 34.0 27.0 34.0 4 34.62745016872781 37.0 35.0 37.0 32.0 37.0 5 34.43375753174864 37.0 35.0 37.0 31.0 37.0 6 34.43352741624808 37.0 35.0 37.0 31.0 37.0 7 34.4658923056317 37.0 35.0 37.0 31.0 37.0 8 34.43044613750622 37.0 35.0 37.0 31.0 37.0 9 36.01618526127099 39.0 37.0 39.0 31.0 39.0 10-11 35.87592273881883 39.0 37.0 39.0 30.5 39.0 12-13 35.8221182744211 39.0 37.0 39.0 30.0 39.0 14-15 37.09087998923144 40.0 38.0 41.0 31.0 41.0 16-17 36.90732998146672 40.0 37.5 41.0 30.5 41.0 18-19 36.55043632638936 40.0 37.0 41.0 30.0 41.0 20-21 36.260833770639145 40.0 36.0 41.0 29.0 41.0 22-23 35.978637817123264 40.0 36.0 41.0 27.0 41.0 24-25 35.67538595182162 40.0 36.0 41.0 26.0 41.0 26-27 35.313976387998444 40.0 36.0 41.0 23.0 41.0 28-29 35.02320236102743 40.0 36.0 41.0 18.5 41.0 30-31 34.63938730236643 40.0 35.5 41.0 13.0 41.0 32-33 34.5350670487165 40.0 35.0 41.0 10.0 41.0 34-35 34.58009412987998 40.0 35.0 41.0 9.0 41.0 36-37 34.49635352115972 40.0 35.0 41.0 8.0 41.0 38-39 34.4007108339257 40.0 35.0 41.0 7.5 41.0 40-41 34.13935719342939 40.0 35.0 41.0 4.0 41.0 42-43 33.95452150068595 40.0 35.0 41.0 2.0 41.0 44-45 34.013295186055956 40.0 35.0 41.0 2.0 41.0 46-47 33.90443997494874 40.0 35.0 41.0 2.0 41.0 48-49 33.84709695478132 40.0 35.0 41.0 2.0 41.0 50-51 33.7327967367251 40.0 34.0 41.0 2.0 41.0 52-53 33.58469701890904 39.5 34.0 41.0 2.0 41.0 54-55 33.3172759957893 39.0 33.0 41.0 2.0 41.0 56-57 33.26338024282171 39.0 34.0 41.0 2.0 41.0 58-59 33.21634480330738 39.0 34.0 41.0 2.0 41.0 60-61 33.06173766880043 39.0 33.5 41.0 2.0 41.0 62-63 32.87696904400866 39.0 33.0 41.0 2.0 41.0 64-65 32.32451733254132 38.0 32.5 40.0 2.0 41.0 66-67 32.09575746038222 37.5 32.0 40.0 2.0 41.0 68-69 31.648961404582305 37.0 32.0 40.0 2.0 41.0 70-71 31.06108837369501 36.5 31.0 39.5 2.0 41.0 72-73 30.427092563273128 36.0 30.5 39.0 2.0 41.0 74-75 30.0016850111352 36.0 30.0 39.0 2.0 41.0 76-77 28.881293543136486 34.5 28.5 37.0 2.0 39.0 78-79 29.32105239057109 35.0 29.5 37.0 2.0 39.0 80-81 29.136650774879207 35.0 30.0 37.0 2.0 39.0 82-83 28.80408684854192 35.0 29.0 36.5 2.0 39.0 84-85 28.505748235741905 35.0 29.0 36.0 2.0 38.0 86-87 28.179523702917198 35.0 29.0 36.0 2.0 37.0 88-89 27.890538036891023 35.0 29.0 35.5 2.0 37.0 90-91 27.638463062026297 35.0 28.5 35.0 2.0 36.0 92-93 27.39035021914753 34.5 27.5 35.0 2.0 36.0 94-95 27.165525696937493 34.0 27.0 35.0 2.0 36.0 96-97 26.913321453846336 34.0 26.0 35.0 2.0 36.0 98-99 26.626845830936787 34.0 25.5 35.0 2.0 35.5 100-101 25.573624280663342 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.414661565530377 1101 2 0.8263941503624679 1101 3 0.7471182277805113 1101 4 0.6394055885895398 1101 5 0.6288868875508058 1101 6 0.6000700612045051 1101 7 0.5980093595726146 1101 8 0.6139704428571946 1101 9 0.7311617836963009 1101 10-11 0.5920700698390604 1101 12-13 0.715766514907358 1101 14-15 0.8264766423755248 1101 16-17 0.8140361159881735 1101 18-19 0.7981441021279139 1101 20-21 0.7364692111908155 1101 22-23 0.7556685956648934 1101 24-25 0.7725262780248343 1101 26-27 0.7502468613074456 1101 28-29 0.8132400245006508 1101 30-31 0.9216618440046958 1101 32-33 0.812393116878205 1101 34-35 0.7863818279133312 1101 36-37 0.7451600939030314 1101 38-39 0.7725810571887379 1101 40-41 0.8438039240761057 1101 42-43 0.7716375393354298 1101 44-45 0.7445440704510489 1101 46-47 0.7524418824977204 1101 48-49 0.7523856171569179 1101 50-51 0.6737811119737245 1101 52-53 0.6672424198748104 1101 54-55 0.6767331793461651 1101 56-57 0.6611561875129013 1101 58-59 0.7166419726087767 1101 60-61 0.6469237257991054 1101 62-63 0.6875245954323219 1101 64-65 0.5379931924196129 1101 66-67 0.5977358213505539 1101 68-69 0.5028425203217992 1101 70-71 0.48810717427898354 1101 72-73 0.5201466846535396 1101 74-75 0.6479924321974302 1101 76-77 0.6038534895493974 1101 78-79 0.6268754461905992 1101 80-81 0.5901489185849833 1101 82-83 0.6300642065624231 1101 84-85 0.6454465755078829 1101 86-87 0.6977521002417433 1101 88-89 0.6659653689795526 1101 90-91 0.6866323128619349 1101 92-93 0.730650727702546 1101 94-95 0.7634060919893138 1101 96-97 0.7542721284018228 1101 98-99 0.7087628693977308 1101 100-101 0.6961938919133104 1102 1 1.4597621433783345 1102 2 0.8474522963708502 1102 3 0.7595199061242468 1102 4 0.6717777124605036 1102 5 0.675870073468019 1102 6 0.6900858916640757 1102 7 0.6555135613891849 1102 8 0.6373461775861387 1102 9 0.7266399407434534 1102 10-11 0.7474625914845703 1102 12-13 0.749987478798829 1102 14-15 0.8601252159904789 1102 16-17 0.8594826960481825 1102 18-19 0.8780339292537818 1102 20-21 0.7736886718058926 1102 22-23 0.8159959299163759 1102 24-25 0.8246679892468975 1102 26-27 0.8273346895733695 1102 28-29 0.8146909516500287 1102 30-31 0.9443913548312395 1102 32-33 0.8742807686987604 1102 34-35 0.8087777203092585 1102 36-37 0.7940516960621053 1102 38-39 0.8295307228064033 1102 40-41 0.9402338601036817 1102 42-43 0.7867250710906504 1102 44-45 0.8029616790267582 1102 46-47 0.8233246326837786 1102 48-49 0.8119981649888999 1102 50-51 0.802900765175707 1102 52-53 0.7645671685947519 1102 54-55 0.755103871558866 1102 56-57 0.6990566622942467 1102 58-59 0.8131667683600838 1102 60-61 0.7541788786975019 1102 62-63 0.8250096546020984 1102 64-65 0.6919396809348726 1102 66-67 0.7614376761286223 1102 68-69 0.6362412974088016 1102 70-71 0.6374973296725521 1102 72-73 0.6430875081051575 1102 74-75 0.6703018415152329 1102 76-77 0.5969470454994941 1102 78-79 0.7136856402516507 1102 80-81 0.7687562924895879 1102 82-83 0.7493475740066451 1102 84-85 0.6930650494651616 1102 86-87 0.6722345055571246 1102 88-89 0.6861940013449086 1102 90-91 0.695840957221737 1102 92-93 0.7129541311254819 1102 94-95 0.7103179874677821 1102 96-97 0.7461133733376997 1102 98-99 0.7447999232715823 1102 100-101 0.7856752967454952 1103 1 1.3804013052744075 1103 2 0.7907214184395457 1103 3 0.6964966292499426 1103 4 0.5899557288973583 1103 5 0.536953720744421 1103 6 0.5627355051699823 1103 7 0.545325806036594 1103 8 0.5739291009720731 1103 9 0.6756829831326812 1103 10-11 0.6892244301647779 1103 12-13 0.7033588785548375 1103 14-15 0.8658349717887148 1103 16-17 0.8853052145222691 1103 18-19 0.8354363406429783 1103 20-21 0.8360852298310206 1103 22-23 0.8629030593096019 1103 24-25 0.8628924575928423 1103 26-27 0.8576725315207199 1103 28-29 0.800758807540447 1103 30-31 0.853292735858318 1103 32-33 0.8439205302201032 1103 34-35 0.7807210072172452 1103 36-37 0.801902685474218 1103 38-39 0.8322147000006694 1103 40-41 0.9181096235191859 1103 42-43 0.8446394024712092 1103 44-45 0.8296884472813915 1103 46-47 0.8291567702975939 1103 48-49 0.813652638112444 1103 50-51 0.8056725647572591 1103 52-53 0.7883939704526668 1103 54-55 0.8029358434756162 1103 56-57 0.8323919509990105 1103 58-59 0.7696654298381205 1103 60-61 0.7637684660571935 1103 62-63 0.8032191345723092 1103 64-65 0.7458789074737879 1103 66-67 0.807284788886335 1103 68-69 0.7510295745611444 1103 70-71 0.7448064986910516 1103 72-73 0.7034183079777492 1103 74-75 0.7513839656602066 1103 76-77 0.6745780009242388 1103 78-79 0.7457252315091623 1103 80-81 0.7224722490205586 1103 82-83 0.7391871132463095 1103 84-85 0.7241778334488274 1103 86-87 0.7077327970354972 1103 88-89 0.6909198882071195 1103 90-91 0.6937819077625136 1103 92-93 0.6821103975304581 1103 94-95 0.6861632583639619 1103 96-97 0.7551313737408094 1103 98-99 0.7659358047697289 1103 100-101 0.821952628834282 1104 1 1.3713332332986496 1104 2 0.752008713835199 1104 3 0.6600101725924148 1104 4 0.5828961172499447 1104 5 0.6035887481865814 1104 6 0.5466021363472535 1104 7 0.5459048271692382 1104 8 0.555839846757884 1104 9 0.6784966064083875 1104 10-11 0.6500991830357066 1104 12-13 0.7116135029247914 1104 14-15 0.776246420976122 1104 16-17 0.7758663316465615 1104 18-19 0.7415083110005867 1104 20-21 0.7334864921771143 1104 22-23 0.7472913868633029 1104 24-25 0.7887652640567566 1104 26-27 0.7724973504721291 1104 28-29 0.7609021068272455 1104 30-31 0.8055637948928904 1104 32-33 0.7280593454572539 1104 34-35 0.7821473099675913 1104 36-37 0.7831443482700635 1104 38-39 0.7771291542624823 1104 40-41 0.8449082400797394 1104 42-43 0.809788350715813 1104 44-45 0.7935700390856866 1104 46-47 0.7825779792965051 1104 48-49 0.7115873844610121 1104 50-51 0.7344539805009873 1104 52-53 0.746222533711375 1104 54-55 0.7450950712592999 1104 56-57 0.7470947622261264 1104 58-59 0.7613511934489097 1104 60-61 0.7617658326600107 1104 62-63 0.784490441243598 1104 64-65 0.7453200610007329 1104 66-67 0.7999509422962916 1104 68-69 0.7015253111178197 1104 70-71 0.683713651559188 1104 72-73 0.6846874823973863 1104 74-75 0.687960659226853 1104 76-77 0.6052085460551986 1104 78-79 0.7550065986705761 1104 80-81 0.7458046211867106 1104 82-83 0.7373717642689073 1104 84-85 0.735336494987024 1104 86-87 0.7620841929089295 1104 88-89 0.7413382405200046 1104 90-91 0.7609420435217764 1104 92-93 0.6850371817746606 1104 94-95 0.7517796456192194 1104 96-97 0.7293785494707592 1104 98-99 0.7504059546736102 1104 100-101 0.8201305837029977 1105 1 1.2937558069339126 1105 2 0.6819625435497052 1105 3 0.6101472096276304 1105 4 0.48924757076804326 1105 5 0.5162345315567833 1105 6 0.4446637692246327 1105 7 0.46664590321798016 1105 8 0.4557869492344153 1105 9 0.5007742513763205 1105 10-11 0.5036801309439483 1105 12-13 0.5563757695687457 1105 14-15 0.6811949286220624 1105 16-17 0.6861210448853043 1105 18-19 0.6273637546656019 1105 20-21 0.6371872337700424 1105 22-23 0.6452058587973113 1105 24-25 0.6820142990458251 1105 26-27 0.65368146516861 1105 28-29 0.6541176707675405 1105 30-31 0.6819374633588282 1105 32-33 0.7110807570108122 1105 34-35 0.6091056018592766 1105 36-37 0.6482224227390176 1105 38-39 0.640931366869502 1105 40-41 0.7245677786848432 1105 42-43 0.634273881600457 1105 44-45 0.5822504027017104 1105 46-47 0.6610973907798723 1105 48-49 0.6696927518958304 1105 50-51 0.6459052038041051 1105 52-53 0.6435320165766427 1105 54-55 0.5621684360047894 1105 56-57 0.61472863543095 1105 58-59 0.6604744992463694 1105 60-61 0.6162009239364892 1105 62-63 0.6057185492109411 1105 64-65 0.5912724693257019 1105 66-67 0.5788683231898446 1105 68-69 0.5696518246079805 1105 70-71 0.5691709611322757 1105 72-73 0.5482017892748026 1105 74-75 0.6003740336982553 1105 76-77 0.48171373552164454 1105 78-79 0.5833814372635118 1105 80-81 0.5995624122584537 1105 82-83 0.6203151651361054 1105 84-85 0.6107922859509181 1105 86-87 0.6215567870430512 1105 88-89 0.6392407807215186 1105 90-91 0.6380841827191404 1105 92-93 0.6688704924973656 1105 94-95 0.6941971369965465 1105 96-97 0.6874806175285499 1105 98-99 0.7957266079777305 1105 100-101 0.741673407450925 1106 1 1.1735694744613738 1106 2 0.5536060543183865 1106 3 0.5279573060964822 1106 4 0.5091762594251037 1106 5 0.30979065984656984 1106 6 0.2662733607743206 1106 7 0.342061740428818 1106 8 0.3461295723016278 1106 9 0.36360570008097426 1106 10-11 0.3779686328462546 1106 12-13 0.3988952866010891 1106 14-15 0.449762064695463 1106 16-17 0.5093516604799078 1106 18-19 0.4820588331369251 1106 20-21 0.47053432360052483 1106 22-23 0.4823190067398784 1106 24-25 0.4020420510086282 1106 26-27 0.39256206801017157 1106 28-29 0.37866216913398176 1106 30-31 0.45867845767694604 1106 32-33 0.44622203779540826 1106 34-35 0.36153272176800755 1106 36-37 0.42577191472228293 1106 38-39 0.42025575773161705 1106 40-41 0.5315286995228092 1106 42-43 0.4513258798613222 1106 44-45 0.4194044265383141 1106 46-47 0.4384398063485122 1106 48-49 0.44185452110988876 1106 50-51 0.4600927976220959 1106 52-53 0.4780568536114629 1106 54-55 0.4999925029887393 1106 56-57 0.5189986864444478 1106 58-59 0.5526902739643944 1106 60-61 0.5460442597868749 1106 62-63 0.542179578885289 1106 64-65 0.5289402801124439 1106 66-67 0.6057808659867732 1106 68-69 0.49010848154554765 1106 70-71 0.5098201583054909 1106 72-73 0.4722802158182624 1106 74-75 0.49958767932719894 1106 76-77 0.36767730073738036 1106 78-79 0.4966148862408808 1106 80-81 0.4980430555475017 1106 82-83 0.5439681926446802 1106 84-85 0.5337895887313167 1106 86-87 0.4881391905752608 1106 88-89 0.523670869517769 1106 90-91 0.5335518819432821 1106 92-93 0.5228967221510601 1106 94-95 0.5307462978577959 1106 96-97 0.584361123587783 1106 98-99 0.6143522179472534 1106 100-101 0.5482078884514507 1107 1 1.206112353380643 1107 2 0.6349410978411605 1107 3 0.5526825612131567 1107 4 0.48599471260921234 1107 5 0.41818181994035797 1107 6 0.4166671638252737 1107 7 0.37865697534924436 1107 8 0.41374705567672265 1107 9 0.46191623067583976 1107 10-11 0.4520713475567888 1107 12-13 0.4581941726046139 1107 14-15 0.519242446364288 1107 16-17 0.5644029150422156 1107 18-19 0.5273974862573496 1107 20-21 0.5113102671204217 1107 22-23 0.5330117917875228 1107 24-25 0.4641654909637367 1107 26-27 0.4305828130045626 1107 28-29 0.4581531145824371 1107 30-31 0.4644064851485368 1107 32-33 0.4747154649045484 1107 34-35 0.4463570474199443 1107 36-37 0.435269313179397 1107 38-39 0.4292563302872523 1107 40-41 0.4674506080314771 1107 42-43 0.4772367743604349 1107 44-45 0.5060584147753033 1107 46-47 0.4659082422579601 1107 48-49 0.48886798177495905 1107 50-51 0.368249251714758 1107 52-53 0.3545220513956977 1107 54-55 0.32651853826157407 1107 56-57 0.4206614697074187 1107 58-59 0.4803644984771509 1107 60-61 0.41546141638104217 1107 62-63 0.47093786858625464 1107 64-65 0.4560845548414463 1107 66-67 0.46247422565071616 1107 68-69 0.47009669880063143 1107 70-71 0.46813671048981575 1107 72-73 0.47117177680224387 1107 74-75 0.4688493533351661 1107 76-77 0.3113027159701254 1107 78-79 0.40711997744693207 1107 80-81 0.4209076991891898 1107 82-83 0.485288502120266 1107 84-85 0.45146718369141325 1107 86-87 0.43830533145189676 1107 88-89 0.4197884888438921 1107 90-91 0.4086060652778265 1107 92-93 0.43807483629141686 1107 94-95 0.42725913820583017 1107 96-97 0.43380700948077333 1107 98-99 0.4328732155990558 1107 100-101 0.4152912275029266 1108 1 1.0835365380692998 1108 2 0.5316838208727326 1108 3 0.425067678603007 1108 4 0.3974355581105087 1108 5 0.301028499907531 1108 6 0.2947492876568205 1108 7 0.27237783330789966 1108 8 0.2892096825236621 1108 9 0.3165675626022164 1108 10-11 0.29675068972942853 1108 12-13 0.325649641419659 1108 14-15 0.2977260507125834 1108 16-17 0.2976404557158574 1108 18-19 0.3141316123992439 1108 20-21 0.3091780962046471 1108 22-23 0.29041903733625674 1108 24-25 0.35653644300793275 1108 26-27 0.34376252457434475 1108 28-29 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-3.7683409619430037 2316 84-85 -3.7295930240462525 2316 86-87 -3.6735451376285653 2316 88-89 -3.6795875692644024 2316 90-91 -3.7239214622601793 2316 92-93 -3.732203495933767 2316 94-95 -3.6834610281090114 2316 96-97 -3.67308054229386 2316 98-99 -3.634992659469045 2316 100-101 -3.453413907172745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1195993.0 3 324279.0 4 180442.0 5 183447.0 6 415715.0 7 390471.0 8 325476.0 9 483494.0 10 668578.0 11 571409.0 12 396221.0 13 292023.0 14 258267.0 15 244634.0 16 235548.0 17 228739.0 18 229212.0 19 236595.0 20 251105.0 21 276536.0 22 317930.0 23 371046.0 24 438525.0 25 518428.0 26 629165.0 27 835547.0 28 681187.0 29 594620.0 30 696865.0 31 841640.0 32 1041821.0 33 1334530.0 34 1796746.0 35 2601448.0 36 4046432.0 37 7835346.0 38 1.387743E7 39 5086078.0 40 15360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.39962695717042 21.92490390703657 10.793488660558028 41.88198047523499 2 16.649285729456224 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28.607481659265073 32.52153000003622 19.73976958868598 46-47 20.364652555379255 28.822525154106206 33.0399528571682 17.77286943334634 48-49 18.252659236163456 30.989732884407196 32.74199584633079 18.01561203309856 50-51 19.232474004922736 29.20544511290599 31.379551118111475 20.182529764059794 52-53 20.15786761078559 29.130065223619496 30.8540520047424 19.858015160852517 54-55 18.16610608447365 29.815451185613558 31.499549355498317 20.518893374414475 56-57 18.465461868371413 29.622462828312173 33.85065808742882 18.061417215887595 58-59 18.596850638599534 29.188241595858656 32.074344744365945 20.14056302117587 60-61 20.55896332031085 30.039921241963402 29.03932373313377 20.36179170459198 62-63 18.804197783104332 29.42327613496835 33.66700156147996 18.105524520447364 64-65 20.87324947145988 30.98831035048023 29.93209806308504 18.206342114974852 66-67 18.83403677225682 34.529915773649996 28.37297435719195 18.263073096901227 68-69 18.970212435305907 33.65989218013881 29.88063711116765 17.489258273387627 70-71 19.155792094264953 33.60023075904631 29.169699272243367 18.074277874445368 72-73 18.3890054398313 34.39795930805426 28.87640167581327 18.33663357630117 74-75 18.675396635273913 32.14972196970992 30.51627107198968 18.65861032302648 76-77 18.876374336641604 32.31205555116233 30.20592733764792 18.605642774548144 78-79 18.739768334826458 32.74079262645092 29.463154638971456 19.05628439975116 80-81 19.16676286608295 31.812849532726062 29.977171289539434 19.043216311651555 82-83 19.330705968778123 31.31842463322578 30.400020136040588 18.950849261955508 84-85 19.43011817690117 31.481208410103985 29.834250000245405 19.254423412749443 86-87 19.522732682776375 31.498229270025668 29.65719779234374 19.321840254854212 88-89 19.459808396774182 31.306317467234624 30.07888003953681 19.15499409645438 90-91 19.6964812830082 31.490350811977713 30.099334851037185 18.71383305397691 92-93 19.75743591998725 31.17156132135849 30.064962460157354 19.006040298496917 94-95 19.681676024365707 31.22429642466959 30.07275033320338 19.021277217761323 96-97 19.516451835551628 31.4625941482121 29.545729044641277 19.47522497159499 98-99 19.42784242705611 31.18863835332461 30.37396449265054 19.00955472696874 100-101 19.51733496357384 32.12596623575475 29.011164605613722 19.345534195057688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34142.0 1 33350.5 2 26789.5 3 24493.5 4 31507.0 5 40870.5 6 49741.0 7 59136.0 8 71460.0 9 82069.5 10 90736.5 11 100591.5 12 112771.0 13 123540.5 14 135225.0 15 147513.0 16 158934.5 17 170078.5 18 191010.5 19 220739.5 20 261497.5 21 311358.0 22 360934.5 23 430867.5 24 526275.0 25 615204.5 26 709432.0 27 842983.5 28 1161287.0 29 1373111.0 30 1495644.0 31 1723689.0 32 2125126.5 33 2228640.5 34 1930145.0 35 2018489.5 36 2167409.0 37 2250481.0 38 2478530.0 39 2300854.5 40 2155955.5 41 2047533.5 42 1729568.0 43 1639775.5 44 1502945.5 45 1362151.0 46 1266310.5 47 1181280.0 48 1108728.5 49 1041451.0 50 948711.5 51 850987.0 52 767724.5 53 689770.0 54 614657.0 55 537069.0 56 471783.0 57 393289.0 58 326589.5 59 272082.0 60 204890.0 61 146874.5 62 106198.0 63 81284.0 64 61931.5 65 45171.5 66 34561.5 67 28943.0 68 22097.5 69 14767.0 70 12437.0 71 12425.5 72 11182.0 73 13125.5 74 13119.0 75 8110.0 76 5473.0 77 5333.0 78 4577.0 79 2424.0 80 1576.5 81 1114.5 82 813.0 83 584.5 84 435.5 85 300.5 86 202.5 87 140.0 88 109.5 89 79.5 90 37.5 91 26.0 92 17.5 93 9.5 94 5.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051081166000187486 2 0.007117014713417092 3 0.006170958151953485 4 0.003968726903069322 5 0.03895319194773183 6 0.12007067238791427 7 0.13532730652122676 8 0.14390658708171936 9 0.14657792106543713 10-11 0.15722596431427543 12-13 0.16292487557197166 14-15 0.16575225000514246 16-17 0.17520692730093124 18-19 0.17347281740040615 20-21 0.17652002240387554 22-23 0.149209999590173 24-25 0.16916649355009256 26-27 0.17493410186100708 28-29 0.17697636711454004 30-31 0.19087083682118086 32-33 0.19959143703400825 34-35 0.19749715829732428 36-37 0.19092285030433187 38-39 0.2199689457915086 40-41 0.24365372696823337 42-43 0.254867048826411 44-45 0.2878524296224206 46-47 0.2718097049229957 48-49 0.26627664797949796 50-51 0.2608319158187095 52-53 0.2571252191043443 54-55 0.2584275189560686 56-57 0.27373518518605755 58-59 0.2651824020603777 60-61 0.27289806252326865 62-63 0.28130658183719004 64-65 0.3866221085802855 66-67 0.1868177106813005 68-69 0.3244287035287988 70-71 0.006698944075259938 72-73 0.004080604961167715 74-75 0.01573162518699338 76-77 0.03458504074952175 78-79 0.017141877550917863 80-81 0.02447185312931172 82-83 0.029008802801143933 84-85 0.02351009438425536 86-87 0.019377475939936634 88-89 0.02851909095034483 90-91 0.020442280264820464 92-93 0.02050705177214059 94-95 0.031103081537827895 96-97 0.03284013559777663 98-99 0.017638459107038804 100-101 0.0281265363605259 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0948328E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.465009628500457 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15507003448909 15.212891907374576 2 8.362718024132691 3.2556077372035426 3 3.2937199061735876 1.9233686906175749 4 1.903763137503076 1.4822707120752643 5 1.2486478097502416 1.215247081969723 6 0.8933609673093226 1.0433567898241467 7 0.6606379302393771 0.9001526571143461 8 0.5263257942326346 0.8195949321973105 9 0.4183690393371851 0.7329201641068315 >10 3.4435037860524744 13.894524780723778 >50 0.5418062946875163 7.402552879506799 >100 0.46677789378260964 18.20959871215172 >500 0.050729689981851546 6.7666339310609755 >1k 0.03025958147534265 11.013285830634228 >5k 0.0023715921187938962 3.1421390744163045 >10k+ 0.001938518734265099 12.985854119022846 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG 1452969 2.8518482490730612 TruSeq Adapter, Index 9 (100% over 49bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC 225018 0.4416592434593732 TruSeq Adapter, Index 9 (100% over 50bp) GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 153581 0.3014446322949008 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 124078 0.24353694197776227 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 116609 0.2288769908209745 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 109802 0.21551639535648745 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 96283 0.1889816678576773 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 81685 0.160329108346794 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 76828 0.15079592013304147 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 73817 0.14488601078331756 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 67745 0.13296805343641505 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 67642 0.13276588782265827 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 67006 0.13151756422703412 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66062 0.1296647065630888 No Hit ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT 63142 0.12393340955173249 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 61162 0.12004711911252515 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 60506 0.11875954005791907 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 60203 0.1181648198543434 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 59627 0.11703426263566491 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 59529 0.11684191088665365 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 53614 0.10523210889275897 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 53303 0.10462168650559053 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 51740 0.10155387238615562 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 51659 0.10139488777727898 No Hit GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT 51293 0.10067651287791035 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.3892672591728625 0.0 0.0 0.0 0.0 2 3.4315964205930367 0.0 0.0 0.0 0.0 3 3.431928129221434 0.0 0.0 0.0 0.0 4 3.436646635391057 0.0 0.0 0.0 0.0 5 3.4373905263387643 0.0 0.0 1.96277294909462E-6 0.0 6 3.4382050771126385 0.0 0.0 1.96277294909462E-6 0.0 7 3.439357224833757 0.0 0.0 1.96277294909462E-6 0.0 8 3.4460483178172208 0.0 0.0 3.92554589818924E-6 0.0 9 3.4467097723010656 0.0 0.0 7.85109179637848E-6 0.0 10-11 3.451102458161139 1.96277294909462E-6 0.0 8.832478270925789E-6 0.0 12-13 3.4544902042712766 1.96277294909462E-6 0.0 9.8138647454731E-6 1.96277294909462E-6 14-15 3.4572488816512292 1.96277294909462E-6 0.0 9.8138647454731E-6 1.96277294909462E-6 16-17 3.4602676264469365 1.96277294909462E-6 0.0 1.2758024169115028E-5 1.96277294909462E-6 18-19 3.4628172685078105 1.96277294909462E-6 0.0 1.7664956541851578E-5 2.9441594236419303E-6 20-21 3.4782446638876943 1.96277294909462E-6 0.0 1.96277294909462E-5 3.92554589818924E-6 22-23 3.4936995380888654 3.92554589818924E-6 0.0 2.159050244004082E-5 3.92554589818924E-6 24-25 3.4961422090240135 3.92554589818924E-6 0.0 2.159050244004082E-5 3.92554589818924E-6 26-27 3.4981462002050394 3.92554589818924E-6 0.0 2.2571888914588128E-5 3.92554589818924E-6 28-29 3.500033406395594 3.92554589818924E-6 0.0 2.355327538913544E-5 3.92554589818924E-6 30-31 3.50212179681343 3.92554589818924E-6 0.0 2.7478821287324678E-5 3.92554589818924E-6 32-33 3.504675364420202 5.88831884728386E-6 0.0 2.7478821287324678E-5 3.92554589818924E-6 34-35 3.5074006746600204 5.88831884728386E-6 0.0 3.042298071096661E-5 3.92554589818924E-6 36-37 3.509777592701374 5.88831884728386E-6 0.0 3.336714013460854E-5 3.92554589818924E-6 38-39 3.5122889606897405 5.88831884728386E-6 0.0 3.434852660915585E-5 3.92554589818924E-6 40-41 3.515004457064813 5.88831884728386E-6 0.0 3.827407250734509E-5 3.92554589818924E-6 42-43 3.5186709169337216 5.88831884728386E-6 0.0 4.5143777829176256E-5 3.92554589818924E-6 44-45 3.521705363913022 5.88831884728386E-6 0.0 4.90693237273655E-5 3.92554589818924E-6 46-47 3.5251166632985482 5.88831884728386E-6 0.0 6.182734789648053E-5 3.92554589818924E-6 48-49 3.5285220743652275 5.88831884728386E-6 0.0 6.477150732012246E-5 3.92554589818924E-6 50-51 3.5336262654193478 6.8697053218311695E-6 0.0 6.477150732012246E-5 3.92554589818924E-6 52-53 3.54240182327475 7.85109179637848E-6 0.0 6.575289379466976E-5 3.92554589818924E-6 54-55 3.5475020887829727 7.85109179637848E-6 0.0 6.673428026921708E-5 3.92554589818924E-6 56-57 3.552631795885431 7.85109179637848E-6 0.0 7.262259911650094E-5 3.92554589818924E-6 58-59 3.557861604408294 7.85109179637848E-6 0.0 8.047369091287941E-5 3.92554589818924E-6 60-61 3.5632032909892546 7.85109179637848E-6 0.0 8.439923681106866E-5 3.92554589818924E-6 62-63 3.56918680432457 7.85109179637848E-6 0.0 8.734339623471059E-5 3.92554589818924E-6 64-65 3.577621821073304 7.85109179637848E-6 0.0 8.83247827092579E-5 3.92554589818924E-6 66-67 3.5857319988989627 7.85109179637848E-6 0.0 9.126894213289983E-5 3.92554589818924E-6 68-69 3.5947921588319836 7.85109179637848E-6 0.0 9.617587450563638E-5 3.92554589818924E-6 70-71 3.604097665383641 7.85109179637848E-6 0.0 1.0010142040382561E-4 3.92554589818924E-6 72-73 3.6166937607844556 1.079525122002041E-5 0.0 1.177663769456772E-4 5.88831884728386E-6 74-75 3.629080820866192 1.177663769456772E-5 0.0 1.2856162816569762E-4 5.88831884728386E-6 76-77 3.642638675012063 1.177663769456772E-5 1.96277294909462E-6 1.5211490355483305E-4 6.8697053218311695E-6 78-79 3.6567147012164956 1.177663769456772E-5 1.96277294909462E-6 1.5505906297847498E-4 7.85109179637848E-6 80-81 3.6720665298378385 1.177663769456772E-5 1.96277294909462E-6 1.5996599535121153E-4 7.85109179637848E-6 82-83 3.6909650891781967 1.177663769456772E-5 1.96277294909462E-6 2.060911596549351E-4 8.832478270925789E-6 84-85 3.7118087565111066 1.177663769456772E-5 3.92554589818924E-6 2.1296086497676626E-4 9.8138647454731E-6 86-87 3.7345553322181644 1.177663769456772E-5 3.92554589818924E-6 2.159050244004082E-4 9.8138647454731E-6 88-89 3.760468842078586 1.177663769456772E-5 3.92554589818924E-6 2.2375611619678665E-4 9.8138647454731E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 207260 0.0 81.48013 1 GATCGGA 240750 0.0 71.8212 2 ATCGGAA 244580 0.0 70.670944 3 TCGGAAG 246200 0.0 70.01881 4 CGGAAGA 248625 0.0 69.4757 5 GAAGAGC 270095 0.0 63.851116 7 AGAGCAC 272905 0.0 63.205902 9 GGAAGAG 279210 0.0 61.930267 6 CGCGTGT 48880 0.0 61.090042 1 AAGAGCA 290410 0.0 59.423836 8 GGATCGG 6505 0.0 56.56847 1 GCGTGTT 59455 0.0 50.54481 2 GTTTTTT 625020 0.0 41.596596 1 CTCGTAT 228015 0.0 41.24472 42-43 ATCTCGT 225105 0.0 40.502003 40-41 ACGATCA 250240 0.0 40.37986 32-33 TATGCCG 244715 0.0 40.230995 46-47 CGTCTGA 249205 0.0 40.193165 16-17 TGCCGTC 241580 0.0 40.053276 48-49 AGTCACG 247960 0.0 39.937534 28-29 >>END_MODULE