FastQCFastQC Report
Fri 27 May 2016
SRR1233085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42768573
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1270510.2970662593769495Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT824130.19269522974264303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT341300.073.825521
GCGTGTT488500.051.4263842
GTTTTTT8027850.046.9895251
AGATCGG376600.042.5647851
CGCCGTA267200.039.9752846-47
AGAGCGT401100.039.2891169
GTCGCCG270200.038.84074444-45
CCGTATC277000.038.784648-49
AAGAGCG422100.038.380028
GATCGGA426250.037.7819672
ATCGGAA435500.037.3070143
CGGAAGA446300.036.4916465
TCGGAAG444150.036.4612664
TCCGATC32600.035.843521
TCGGTGG312900.034.4244438-39
CGTGTTT759400.033.8072743
CCGATCT36000.033.802052
TCTCGGT313600.033.57095736-37
TGGTCGC311950.031.93749642-43
CTCGTAT31850.031.2967381
Produced by FastQC (version 0.11.5)