##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233085_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42768573 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10799111768354 33.0 31.0 34.0 28.0 34.0 2 31.418512420323214 33.0 31.0 34.0 28.0 34.0 3 31.361032129830473 34.0 31.0 34.0 27.0 34.0 4 34.699419735140566 37.0 35.0 37.0 32.0 37.0 5 34.66251747515635 37.0 35.0 37.0 32.0 37.0 6 34.69597659945306 37.0 35.0 37.0 32.0 37.0 7 34.817414553438574 37.0 35.0 37.0 32.0 37.0 8 34.79874519077361 37.0 35.0 37.0 32.0 37.0 9 36.36649768511098 39.0 37.0 39.0 32.0 39.0 10-11 36.251233937592445 39.0 37.0 39.0 32.0 39.0 12-13 36.174577837796924 39.0 37.0 39.0 32.0 39.0 14-15 37.38168756530642 40.0 38.0 41.0 31.5 41.0 16-17 37.071718876381496 40.0 37.5 41.0 31.0 41.0 18-19 36.55714592114168 40.0 36.0 41.0 29.5 41.0 20-21 36.09773436911257 40.0 35.0 41.0 27.5 41.0 22-23 35.51066807863802 39.0 35.0 41.0 26.0 41.0 24-25 34.98596408629299 39.0 35.0 41.0 20.5 41.0 26-27 34.525263421812085 39.5 35.0 41.0 14.5 41.0 28-29 34.07457362442277 40.0 34.0 41.0 9.5 41.0 30-31 33.71305249066879 39.5 34.0 41.0 6.0 41.0 32-33 33.45948326356365 39.0 34.0 41.0 2.0 41.0 34-35 33.25916037694313 39.0 34.0 41.0 2.0 41.0 36-37 33.085774512514135 39.0 33.0 41.0 2.0 41.0 38-39 32.9198050867865 39.0 33.0 41.0 2.0 41.0 40-41 32.774491400496345 39.0 33.0 41.0 2.0 41.0 42-43 32.59389033157595 39.0 33.0 41.0 2.0 41.0 44-45 32.300503023563586 39.0 32.0 41.0 2.0 41.0 46-47 32.18476338922975 39.0 31.0 41.0 2.0 41.0 48-49 32.17738257247909 39.0 32.0 41.0 2.0 41.0 50-51 31.54773024575779 38.0 31.0 40.0 2.0 40.5 52-53 31.550527919180283 38.0 31.0 40.0 2.0 40.5 54-55 31.815464979390356 39.0 31.0 41.0 2.0 41.0 56-57 31.7230523099286 39.0 31.0 41.0 2.0 41.0 58-59 31.557856816966982 38.0 31.0 41.0 2.0 41.0 60-61 31.45349695207273 38.0 31.0 41.0 2.0 41.0 62-63 31.240709422313437 38.0 31.0 41.0 2.0 41.0 64-65 30.942623091492905 38.0 30.0 40.0 2.0 41.0 66-67 30.60030752019713 37.0 29.0 40.0 2.0 41.0 68-69 30.300459966246713 37.0 29.0 40.0 2.0 41.0 70-71 29.971416874254842 36.0 28.5 39.5 2.0 41.0 72-73 29.642427337942745 36.0 28.0 39.0 2.0 41.0 74-75 29.268952917835254 35.0 28.0 39.0 2.0 41.0 76-77 28.895120162180767 35.0 27.0 38.5 2.0 40.0 78-79 28.5085493617007 35.0 26.0 37.0 2.0 39.5 80-81 28.123097162956547 35.0 26.0 37.0 2.0 39.0 82-83 27.749749448035125 35.0 26.0 36.5 2.0 39.0 84-85 27.37374236217795 34.5 25.0 36.0 2.0 38.0 86-87 27.02082227527208 34.0 24.5 36.0 2.0 37.0 88-89 26.691890479955923 34.0 23.5 35.0 2.0 37.0 90-91 26.392693427017075 34.0 21.5 35.0 2.0 36.0 92-93 26.065051597583114 34.0 20.0 35.0 2.0 36.0 94-95 25.748469805153427 34.0 17.5 35.0 2.0 36.0 96-97 25.39369579854815 34.0 4.5 35.0 2.0 36.0 98-99 24.97507979749523 34.0 2.0 35.0 2.0 35.0 100-101 22.92674057654437 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5971152980081342 1101 2 0.6446777734839237 1101 3 0.6890405329010072 1101 4 0.7896734184787704 1101 5 0.7902849668100629 1101 6 0.6924917404565676 1101 7 0.6856426706731966 1101 8 0.6167225947904384 1101 9 0.7780671520141667 1101 10-11 0.7189232978629434 1101 12-13 0.6315950848648697 1101 14-15 0.8092925772497637 1101 16-17 0.8893171936126194 1101 18-19 0.8873302790942788 1101 20-21 0.95882859398683 1101 22-23 0.9505186757106756 1101 24-25 0.9262916925924998 1101 26-27 0.8263203242147839 1101 28-29 0.9357934513754245 1101 30-31 0.9479490331984834 1101 32-33 0.8733231291298367 1101 34-35 0.87464969556612 1101 36-37 0.8479425667046243 1101 38-39 0.8712850843112818 1101 40-41 0.8876274051085744 1101 42-43 0.8977852550116481 1101 44-45 0.9164404815959841 1101 46-47 0.8823231176684558 1101 48-49 0.878098462069083 1101 50-51 0.8870705396485334 1101 52-53 0.9461913741737966 1101 54-55 0.9906216569231496 1101 56-57 0.9481276003673926 1101 58-59 0.9782505856660286 1101 60-61 0.9727483196824629 1101 62-63 0.9981178785082285 1101 64-65 0.9468217594434662 1101 66-67 0.9469515247019409 1101 68-69 0.8581607056298672 1101 70-71 0.7705659371131404 1101 72-73 0.691320815262614 1101 74-75 0.8445344626879248 1101 76-77 0.8789191137450452 1101 78-79 0.8297663235236108 1101 80-81 0.8632444919617122 1101 82-83 0.9044255669420949 1101 84-85 0.8532880368080562 1101 86-87 0.8205381282726272 1101 88-89 0.8817061729877196 1101 90-91 0.8420715964144243 1101 92-93 0.7378380380707341 1101 94-95 0.8600730271368384 1101 96-97 0.9625647971354532 1101 98-99 0.9955588498357315 1101 100-101 1.1673532801237414 1102 1 0.6161018455188731 1102 2 0.6866419088715183 1102 3 0.7191505784037453 1102 4 0.7293532452784106 1102 5 0.78025080228322 1102 6 0.6757678406785743 1102 7 0.6341491894014624 1102 8 0.6408824682756844 1102 9 0.7551625894890606 1102 10-11 0.7604973271413442 1102 12-13 0.7727661502109058 1102 14-15 0.9085300312998754 1102 16-17 0.9374557449871332 1102 18-19 0.8676526340245232 1102 20-21 0.8253179122092646 1102 22-23 0.7931143910730185 1102 24-25 0.8670340574860091 1102 26-27 0.8657992070430467 1102 28-29 0.8804612997102765 1102 30-31 0.8724027709383293 1102 32-33 0.7738507953583778 1102 34-35 0.8068056347921413 1102 36-37 0.7724345375611534 1102 38-39 0.7496074142720559 1102 40-41 0.8369816529202225 1102 42-43 0.7982678986444327 1102 44-45 0.8451778225298625 1102 46-47 0.8463953664004791 1102 48-49 0.8213810270616833 1102 50-51 0.8319364573025112 1102 52-53 0.8395617814026686 1102 54-55 0.9291029055618729 1102 56-57 0.8855838134458338 1102 58-59 0.9423174412665354 1102 60-61 0.8833106327325702 1102 62-63 0.9023777455515933 1102 64-65 0.8824089695572646 1102 66-67 0.8842343489867197 1102 68-69 0.8318308091659965 1102 70-71 0.7695398314958055 1102 72-73 0.7871095303477276 1102 74-75 0.7734306466706613 1102 76-77 0.7823259684356323 1102 78-79 0.7915477020426529 1102 80-81 0.7024771025864176 1102 82-83 0.7802800574897084 1102 84-85 0.7572108183003969 1102 86-87 0.691219155518084 1102 88-89 0.7703748384946003 1102 90-91 0.715168526854022 1102 92-93 0.6930090589182178 1102 94-95 0.7506882143208635 1102 96-97 0.770379686615982 1102 98-99 0.7935002470425871 1102 100-101 0.8823092718409988 1103 1 0.5247541983867663 1103 2 0.5812600606856897 1103 3 0.5659748916882812 1103 4 0.659983522684854 1103 5 0.6873080225619219 1103 6 0.6318841278077301 1103 7 0.5866605123555217 1103 8 0.5699456908363345 1103 9 0.6533618793819187 1103 10-11 0.6923850821381308 1103 12-13 0.7381536126409216 1103 14-15 0.8613195928938495 1103 16-17 0.8799915329119017 1103 18-19 0.8221862460957468 1103 20-21 0.7823867182082793 1103 22-23 0.8192091346241028 1103 24-25 0.8346761512254375 1103 26-27 0.8515203303342176 1103 28-29 0.7103522084039611 1103 30-31 0.7438730455658131 1103 32-33 0.8065788972789463 1103 34-35 0.7588420926629027 1103 36-37 0.7817540655096025 1103 38-39 0.8165625817372018 1103 40-41 0.8108299297136696 1103 42-43 0.7398144757093732 1103 44-45 0.810484571373081 1103 46-47 0.7555779376476082 1103 48-49 0.6958221933683788 1103 50-51 0.7518517580146806 1103 52-53 0.7498281259662072 1103 54-55 0.7996092037795783 1103 56-57 0.766381842465961 1103 58-59 0.9052133676679226 1103 60-61 0.8244832796785424 1103 62-63 0.8074827505480293 1103 64-65 0.7561283225468607 1103 66-67 0.7859530405201518 1103 68-69 0.7723374207592251 1103 70-71 0.8018120235039206 1103 72-73 0.7719615161810083 1103 74-75 0.7080680928516117 1103 76-77 0.78909013744126 1103 78-79 0.7367667563457196 1103 80-81 0.6647387423853068 1103 82-83 0.7444662134133502 1103 84-85 0.7106341805032024 1103 86-87 0.7210084608377763 1103 88-89 0.7722347553914304 1103 90-91 0.7501049715801926 1103 92-93 0.7586474965588579 1103 94-95 0.7701051036098505 1103 96-97 0.7925041798959853 1103 98-99 0.8349924021044899 1103 100-101 0.900889267278032 1104 1 0.42555041360443724 1104 2 0.5496752770835336 1104 3 0.59706866666615 1104 4 0.6101943204042684 1104 5 0.5837421323619267 1104 6 0.5753344443110322 1104 7 0.5696650573179198 1104 8 0.504857116218318 1104 9 0.5769074079228531 1104 10-11 0.5486575792206807 1104 12-13 0.5888648712846205 1104 14-15 0.6753133662606743 1104 16-17 0.7021153289889952 1104 18-19 0.6910257459395339 1104 20-21 0.7405232528063621 1104 22-23 0.765523598432182 1104 24-25 0.6653965297979809 1104 26-27 0.7389061985932912 1104 28-29 0.6900993690809969 1104 30-31 0.6889618939777691 1104 32-33 0.7663714400664219 1104 34-35 0.7330417425382336 1104 36-37 0.7164504706466559 1104 38-39 0.7147704652652891 1104 40-41 0.7352397479048491 1104 42-43 0.7312613061381015 1104 44-45 0.7638108113576649 1104 46-47 0.7718823848130896 1104 48-49 0.8061531799589119 1104 50-51 0.675344102971124 1104 52-53 0.744582946013697 1104 54-55 0.8270401247725054 1104 56-57 0.8075868480865793 1104 58-59 0.9012907563273806 1104 60-61 0.8654853246703276 1104 62-63 0.8262853549787685 1104 64-65 0.7123169462867445 1104 66-67 0.8046372622037516 1104 68-69 0.8489159128359525 1104 70-71 0.8833495587836069 1104 72-73 0.8201857722300829 1104 74-75 0.8159832378643195 1104 76-77 0.8295969922384963 1104 78-79 0.8111180273704086 1104 80-81 0.789763205197989 1104 82-83 0.7439393657102933 1104 84-85 0.8134360242404526 1104 86-87 0.7796166916689842 1104 88-89 0.7980078900791021 1104 90-91 0.8204199812390236 1104 92-93 0.7691021247699013 1104 94-95 0.8244015477715401 1104 96-97 0.8395261338871087 1104 98-99 0.8915144951643299 1104 100-101 1.0109292248444177 1105 1 0.4710096545963758 1105 2 0.48441157180490535 1105 3 0.44422950854363563 1105 4 0.5477435131179078 1105 5 0.5257326341251769 1105 6 0.5639061217938348 1105 7 0.5544437247979559 1105 8 0.5332185286208002 1105 9 0.5932054655390857 1105 10-11 0.5887504333689293 1105 12-13 0.5524872201324413 1105 14-15 0.5912634038715083 1105 16-17 0.6323170710430617 1105 18-19 0.5610305729704592 1105 20-21 0.5956900454701994 1105 22-23 0.6437110089732627 1105 24-25 0.6124736807942526 1105 26-27 0.7100928063656013 1105 28-29 0.7023845330779679 1105 30-31 0.610432792020255 1105 32-33 0.6095889513811059 1105 34-35 0.6516744073805683 1105 36-37 0.5765031577619268 1105 38-39 0.6236320871150767 1105 40-41 0.6388149388344502 1105 42-43 0.6626323588446112 1105 44-45 0.6645320850192604 1105 46-47 0.6231371178691276 1105 48-49 0.7279690570224204 1105 50-51 0.6274631418232062 1105 52-53 0.6483895609316939 1105 54-55 0.7479681281697701 1105 56-57 0.694622606684618 1105 58-59 0.860058193354515 1105 60-61 0.7981618620363484 1105 62-63 0.7208330593451571 1105 64-65 0.7441871916598792 1105 66-67 0.7707135755787604 1105 68-69 0.7685840563147508 1105 70-71 0.7695970636113927 1105 72-73 0.7584028475154483 1105 74-75 0.7522923433971727 1105 76-77 0.7002984364436209 1105 78-79 0.6856358084917993 1105 80-81 0.6621063446149371 1105 82-83 0.6515623548722509 1105 84-85 0.6328505935407698 1105 86-87 0.6694898085188505 1105 88-89 0.6949974985873837 1105 90-91 0.7359090887443109 1105 92-93 0.6880974925309751 1105 94-95 0.7100259043744117 1105 96-97 0.689978441198491 1105 98-99 0.6561401009958061 1105 100-101 0.7816528454977245 1106 1 0.415896707123828 1106 2 0.43760998467734424 1106 3 0.4926426092331795 1106 4 0.5601945093045231 1106 5 0.5476513604011046 1106 6 0.5446213581730177 1106 7 0.4744863391620626 1106 8 0.4724720901710029 1106 9 0.5340075024170474 1106 10-11 0.49994615795623787 1106 12-13 0.5204998294706584 1106 14-15 0.5871918976478554 1106 16-17 0.5793077689900485 1106 18-19 0.5989735881843856 1106 20-21 0.551997649892769 1106 22-23 0.5308403515989113 1106 24-25 0.5805535309156511 1106 26-27 0.5558461841556905 1106 28-29 0.48808511736085336 1106 30-31 0.5537098195682475 1106 32-33 0.5978496529756754 1106 34-35 0.5831071116320174 1106 36-37 0.4791672657234969 1106 38-39 0.5604812060204907 1106 40-41 0.5516847341253595 1106 42-43 0.5044685360134764 1106 44-45 0.4826280933804483 1106 46-47 0.513558866351616 1106 48-49 0.5405881042531391 1106 50-51 0.4686608314257583 1106 52-53 0.5569519873326136 1106 54-55 0.6902297870998204 1106 56-57 0.5903038128158116 1106 58-59 0.7338033732919023 1106 60-61 0.6984548931755192 1106 62-63 0.6141273255418405 1106 64-65 0.5748179494701482 1106 66-67 0.6164125769590676 1106 68-69 0.6521379099904365 1106 70-71 0.6937170392904584 1106 72-73 0.617707478147075 1106 74-75 0.6374343765642934 1106 76-77 0.6174672473034022 1106 78-79 0.6621897231864189 1106 80-81 0.7071870505116493 1106 82-83 0.6421835967137284 1106 84-85 0.5995494554430536 1106 86-87 0.6502900300164818 1106 88-89 0.6581815215697873 1106 90-91 0.664713756625023 1106 92-93 0.6669327354654477 1106 94-95 0.6405320285625962 1106 96-97 0.575466848104778 1106 98-99 0.6656436606194873 1106 100-101 0.6207255188132379 1107 1 0.3890425574491019 1107 2 0.4221743909443241 1107 3 0.42598141429463965 1107 4 0.48562374901330685 1107 5 0.5023466857302097 1107 6 0.5086786590136683 1107 7 0.4684417600788464 1107 8 0.4244675670802991 1107 9 0.4778313191028474 1107 10-11 0.45652870999938244 1107 12-13 0.4724308931155434 1107 14-15 0.5415657615643426 1107 16-17 0.5044901516181142 1107 18-19 0.4770635796405571 1107 20-21 0.53043730827536 1107 22-23 0.5312186431175974 1107 24-25 0.487037356513099 1107 26-27 0.48600059092632364 1107 28-29 0.548040949002413 1107 30-31 0.5159574127487545 1107 32-33 0.5126477806196235 1107 34-35 0.5030548557567798 1107 36-37 0.4260505834675996 1107 38-39 0.42113100542799486 1107 40-41 0.4550479506111813 1107 42-43 0.4648968301459959 1107 44-45 0.49665228353696733 1107 46-47 0.5038004956204176 1107 48-49 0.4813386167892233 1107 50-51 0.431172888560333 1107 52-53 0.4407675056375133 1107 54-55 0.5672795644781665 1107 56-57 0.4897073428495844 1107 58-59 0.5627813675800688 1107 60-61 0.5221754593905281 1107 62-63 0.517573187993893 1107 64-65 0.45224098384735 1107 66-67 0.49230960270351787 1107 68-69 0.54001555885824 1107 70-71 0.545091720856206 1107 72-73 0.5475460995427 1107 74-75 0.6048602435910659 1107 76-77 0.5768962781563118 1107 78-79 0.507496448622252 1107 80-81 0.46747994674211313 1107 82-83 0.5323490861918181 1107 84-85 0.5488231154356953 1107 86-87 0.4914383710763026 1107 88-89 0.5131309667276973 1107 90-91 0.5452808137689047 1107 92-93 0.5337795110329573 1107 94-95 0.5444286722971654 1107 96-97 0.5624563117961401 1107 98-99 0.45361303815961307 1107 100-101 0.3665026793347117 1108 1 0.23488033137931907 1108 2 0.32958818738574536 1108 3 0.39865017106913214 1108 4 0.36325048740808086 1108 5 0.368517222681632 1108 6 0.3561583415745062 1108 7 0.3687429510364666 1108 8 0.33685924815601 1108 9 0.29597725294602384 1108 10-11 0.3568540063294918 1108 12-13 0.29609306000137536 1108 14-15 0.32601249597825444 1108 16-17 0.376602496750138 1108 18-19 0.35196405229442007 1108 20-21 0.3411526100541593 1108 22-23 0.2924836465800098 1108 24-25 0.2865886741323749 1108 26-27 0.3378660108821805 1108 28-29 0.324318150565702 1108 30-31 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96-97 -3.0701349797613915 2316 98-99 -3.068119578859509 2316 100-101 -2.826023978436048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 432891.0 3 126471.0 4 123939.0 5 144534.0 6 199345.0 7 321177.0 8 553411.0 9 923813.0 10 1058040.0 11 802575.0 12 556355.0 13 423642.0 14 351546.0 15 301612.0 16 274251.0 17 261480.0 18 258463.0 19 262750.0 20 277416.0 21 296824.0 22 323839.0 23 357457.0 24 400513.0 25 415225.0 26 429740.0 27 480260.0 28 541642.0 29 620796.0 30 721274.0 31 852321.0 32 1032982.0 33 1286976.0 34 1677291.0 35 2343607.0 36 3541072.0 37 6241707.0 38 9513831.0 39 4024743.0 40 12762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.834906077220158 16.80435881053262 8.579938332515976 45.78079677973125 2 12.221335710938463 15.482470687877473 61.15475696801699 11.141436633167077 3 17.17747423996109 20.19192013880308 41.72283849276603 20.9077671284698 4 18.652639175271272 27.206904771084368 38.30921061086476 15.831245442779604 5 18.973024118767984 30.293423355001757 37.42153453690143 13.312017989328822 6 15.146412362545325 33.06764749615139 38.587756248521224 13.198183892782062 7 12.31072386619908 18.143676934531587 55.852962909898096 13.692636289371235 8 12.367705237212057 21.007745117542044 49.66450681761385 16.96004282763205 9 13.739873562374994 20.0381388922729 49.04834511262208 17.173642432730027 10-11 17.327800302416723 30.21007664391456 37.582714322603586 14.879408731065125 12-13 16.240575054146912 24.246788060553175 43.466471040468996 16.046165844830917 14-15 16.522640275188998 25.720977797076785 42.138336482306535 15.618045445427683 16-17 17.56655441040951 25.96945391809558 40.291614691030844 16.172376980464065 18-19 17.87212511413409 26.916693174096824 39.251454748832444 15.959726962936644 20-21 17.93667561348818 27.298946770447813 38.063982228528076 16.700395387535927 22-23 18.867729740280843 28.01568318343495 36.79596175215274 16.32062532413147 24-25 18.27923635966376 28.883085808102894 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50-51 18.533544816060136 29.83797302199497 33.105653980709796 18.522828181235095 52-53 18.541841279972097 30.69943599489814 32.495416384255584 18.263306340874173 54-55 18.469814865310763 30.57631880882586 31.900620884686376 19.053245441177 56-57 18.800518139358903 30.58793666194706 31.961201853399245 18.65034334529479 58-59 18.707547897019076 30.90474010009156 31.714212576636164 18.673499426253205 60-61 18.89725751455351 31.603585977584903 30.80300432858012 18.69615217928147 62-63 18.94905981065437 31.46823273429057 31.16536034883745 18.417347106217612 64-65 19.398189480610682 31.18315631579469 30.98444348464755 18.434210718947078 66-67 19.307600365384506 31.505834671059453 30.63944897943971 18.547115984116335 68-69 19.577734397161382 31.09493003121586 31.00391599054217 18.32341958108059 70-71 19.474431575806232 31.01685284129531 30.886104878450855 18.622610704447602 72-73 19.039107140068598 31.43999883126898 30.818389383296086 18.702504645366343 74-75 18.96547831311229 30.942684738761944 31.47588716968607 18.615949778439695 76-77 19.0092912149565 31.088739047659974 31.21882890733939 18.683140830044135 78-79 19.069091932778868 31.210572098245926 30.769381460806805 18.950954508168405 80-81 19.060635252038217 30.802038121460072 31.104632150503246 19.03269447599846 82-83 19.16429742369615 31.122631406047642 30.728163730036517 18.984907440219693 84-85 19.072298312498972 30.909469391208162 30.978603862335547 19.03962843395732 86-87 19.305466633862853 31.29095609618384 30.486856899135994 18.91672037081731 88-89 19.159890005511993 31.157268119284282 30.67902917916553 19.003812696038196 90-91 19.256591089247628 31.14194435408727 30.733011753634866 18.868452803030237 92-93 19.280928746896237 31.10861716566815 30.471143165381154 19.13931092205446 94-95 19.030664687011573 30.99446255086513 30.68180990924443 19.29306285287887 96-97 19.086089849976208 31.521967959246776 30.233819472983175 19.158122717793844 98-99 18.970641417074198 31.36839623481782 30.494777835604285 19.16618451250369 100-101 19.528138546073475 31.687925852860836 29.455422815792442 19.328512785273244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18800.0 1 27616.0 2 33252.0 3 35432.5 4 46772.5 5 59637.0 6 72557.5 7 85920.5 8 101298.0 9 116913.0 10 132632.0 11 147889.5 12 161815.0 13 175013.0 14 187867.0 15 200147.0 16 212170.0 17 227786.5 18 252324.0 19 281170.0 20 311673.0 21 361531.5 22 427645.5 23 501216.0 24 601796.5 25 711925.0 26 826601.0 27 948218.0 28 1127997.5 29 1285195.0 30 1403979.0 31 1534341.0 32 1603360.0 33 1633662.5 34 1603004.5 35 1612859.5 36 1681665.5 37 1675598.5 38 1612913.5 39 1505495.5 40 1407158.5 41 1347135.5 42 1282452.5 43 1207750.0 44 1105078.5 45 1027768.0 46 999660.5 47 968480.0 48 928201.5 49 911046.0 50 851281.5 51 746742.5 52 669882.5 53 617492.5 54 556216.5 55 482829.5 56 426875.0 57 368232.0 58 311756.5 59 261230.5 60 203692.5 61 150323.0 62 112264.5 63 82011.0 64 55486.5 65 37164.5 66 25018.5 67 17826.5 68 13126.0 69 9050.0 70 6971.5 71 6747.5 72 5833.5 73 6677.5 74 6514.5 75 3662.5 76 2675.0 77 2480.0 78 2160.5 79 1279.5 80 762.5 81 479.0 82 293.5 83 175.0 84 103.0 85 65.0 86 47.5 87 34.0 88 21.5 89 14.5 90 11.0 91 12.5 92 8.0 93 3.5 94 4.5 95 4.0 96 2.5 97 1.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6214493057788016 2 0.27939908118982604 3 0.27911850133508076 4 0.2979968492285211 5 0.2705982264126512 6 0.20948793404914398 7 0.09258901390046378 8 0.10464225682722685 9 0.10091054475911553 10-11 0.08529276859436016 12-13 0.09386448315682638 14-15 0.09312913012084832 16-17 0.0893541619917971 18-19 0.10452301038896014 20-21 0.11005627894108135 22-23 0.08667579346170844 24-25 0.10320779231984195 26-27 0.08872636456680469 28-29 0.09596766298468738 30-31 0.10801506049780993 32-33 0.10396769609311023 34-35 0.1068728666724513 36-37 0.10642861523577136 38-39 0.10689274107882907 40-41 0.10158978182414458 42-43 0.09101192130025007 44-45 0.36048315196300795 46-47 0.08421955064995973 48-49 0.0701133984526442 50-51 0.07312963189115522 52-53 0.22238642378832701 54-55 0.28771944296574964 56-57 0.21426363699345313 58-59 0.25096114382867063 60-61 0.07194768925304101 62-63 0.06461403329963802 64-65 0.10077142391447104 66-67 0.09710050414822118 68-69 0.07811460064379515 70-71 0.1055775230097109 72-73 0.1299552360561574 74-75 0.11864903699265346 76-77 0.1070797943153259 78-79 0.09933695940708613 80-81 0.09515748865411058 82-83 0.10236371459015012 84-85 0.08903850965520875 86-87 0.11682176068862526 88-89 0.12514679879546134 90-91 0.15102678314752283 92-93 0.15754675752216468 94-95 0.11223194189808483 96-97 0.09715077470552969 98-99 0.11418197188856405 100-101 0.1066904897668669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.2768573E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.21922811949752 #Duplication Level Percentage of deduplicated Percentage of total 1 78.14939278202121 22.834649350980904 2 8.798525197008772 5.141722296930923 3 3.453899847133852 3.0276086260590485 4 1.9173939447735246 2.2409908426912333 5 1.2276657410203078 1.7935722670682168 6 0.900741928970382 1.579139033962909 7 0.6226468592495932 1.273528243281177 8 0.5353727644585828 1.2514543146945076 9 0.42665426955808616 1.1219857587337776 >10 3.2327259227279805 18.97449359370641 >50 0.403544117291235 8.225727801843629 >100 0.29230986173317797 16.481023896889347 >500 0.02511002311076266 4.986402494051655 >1k 0.012784115995301314 6.994057078137969 >5k 8.700882168305741E-4 1.686951681033294 >10k+ 3.6253673031592384E-4 2.386692719935016 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 127051 0.2970662593769495 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82413 0.19269522974264303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.3788482725388102 0.0 0.0 9.352661824840404E-6 0.0 2 0.38006411857603944 0.0 0.0 1.6367158193470707E-5 0.0 3 0.3802348046543428 0.0 0.0 1.8705323649680808E-5 0.0 4 0.3839735312188228 0.0 0.0 2.104348910589091E-5 0.0 5 0.3842564492390242 0.0 0.0 2.104348910589091E-5 0.0 6 0.38463990837384265 0.0 0.0 2.104348910589091E-5 0.0 7 0.38499530952318656 0.0 0.0 2.571982001831111E-5 0.0 8 0.386435619444212 0.0 0.0 3.039615093073131E-5 0.0 9 0.3867863442626435 0.0 0.0 3.039615093073131E-5 0.0 10-11 0.3885902389121096 0.0 0.0 4.091789548367677E-5 0.0 12-13 0.3911201339357289 0.0 0.0 6.663771550198788E-5 0.0 14-15 0.39323500459087096 2.338165456210101E-6 0.0 1.8822231922491312E-4 0.0 16-17 0.3960326195592263 2.338165456210101E-6 0.0 2.852561856576323E-4 0.0 18-19 0.398755413232983 2.338165456210101E-6 0.0 3.0513059203541817E-4 0.0 20-21 0.40314999520792993 2.338165456210101E-6 0.0 3.2734316386941415E-4 0.0 22-23 0.40698575563884254 2.338165456210101E-6 0.0 3.48386652975305E-4 0.0 24-25 0.409950549437317 2.338165456210101E-6 0.0 3.5657023207204036E-4 0.0 26-27 0.41251668602550756 2.338165456210101E-6 0.0 3.6475381116877575E-4 0.0 28-29 0.4149343491072288 2.338165456210101E-6 0.0 3.659228938968808E-4 0.0 30-31 0.4174396933935579 7.0144963686303025E-6 0.0 3.6943014208119595E-4 0.0 32-33 0.41987255455074457 7.0144963686303025E-6 0.0 3.7176830753740605E-4 0.0 34-35 0.42314598618943866 7.0144963686303025E-6 0.0 3.7410647299361614E-4 0.0 36-37 0.42576005516948157 7.0144963686303025E-6 0.0 3.752755557217212E-4 0.0 38-39 0.4286126170260579 7.0144963686303025E-6 0.0 3.8112096936224643E-4 0.0 40-41 0.4317866766328631 7.0144963686303025E-6 0.0 3.822900520903515E-4 0.0 42-43 0.4348321371395768 1.0521744552945454E-5 0.0 3.8345913481845653E-4 0.0 44-45 0.4382481968710997 1.1690827281050504E-5 0.0 3.95149962099507E-4 0.0 46-47 0.44251301066322696 1.1690827281050504E-5 0.0 3.9631904482761207E-4 0.0 48-49 0.4461827613467487 1.1690827281050504E-5 0.0 4.0917895483676765E-4 0.0 50-51 0.4498688791884639 1.4028992737260605E-5 1.1690827281050505E-6 4.17362533933503E-4 0.0 52-53 0.45380634981672174 1.4028992737260605E-5 2.338165456210101E-6 4.2086978211781814E-4 0.0 54-55 0.4581997627089405 1.4028992737260605E-5 2.338165456210101E-6 4.5360409850475955E-4 0.0 56-57 0.46270423846032926 1.4028992737260605E-5 2.338165456210101E-6 4.816620839792808E-4 0.0 58-59 0.46756411536106196 1.4028992737260605E-5 2.338165456210101E-6 4.991983249008565E-4 0.0 60-61 0.47311959648501717 1.5198075465365655E-5 2.338165456210101E-6 5.237490621910626E-4 0.0 62-63 0.47909828555654643 1.6367158193470707E-5 2.338165456210101E-6 5.366089722002182E-4 0.0 64-65 0.48513776692993704 1.6367158193470707E-5 2.338165456210101E-6 5.412853031126384E-4 0.0 66-67 0.4920002825439137 1.6367158193470707E-5 2.338165456210101E-6 5.447925512969535E-4 0.0 68-69 0.4997080449703103 1.6367158193470707E-5 2.338165456210101E-6 5.494688822093737E-4 0.0 70-71 0.5079547545343633 1.6367158193470707E-5 2.338165456210101E-6 5.57652461306109E-4 0.0 72-73 0.5168958992389108 1.6367158193470707E-5 2.338165456210101E-6 5.693432885871595E-4 0.0 74-75 0.5271265421925582 1.8705323649680808E-5 2.338165456210101E-6 5.775268676838949E-4 0.0 76-77 0.5384362485042463 1.8705323649680808E-5 2.338165456210101E-6 6.231210940799919E-4 0.0 78-79 0.5509407573640579 2.104348910589091E-5 2.338165456210101E-6 6.698844032041939E-4 0.0 80-81 0.5649896245076964 2.338165456210101E-5 2.338165456210101E-6 6.768988995728242E-4 0.0 82-83 0.5805746663560647 2.571982001831111E-5 2.338165456210101E-6 7.376912014342867E-4 0.0 84-85 0.5991361928301886 2.571982001831111E-5 2.338165456210101E-6 7.914690069271191E-4 0.0 86-87 0.6202276143279319 2.571982001831111E-5 2.338165456210101E-6 8.125124960330101E-4 0.0 88-89 0.6442195300741037 2.571982001831111E-5 2.338165456210101E-6 8.288796542264807E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 34130 0.0 73.82552 1 GCGTGTT 48850 0.0 51.426384 2 GTTTTTT 802785 0.0 46.989525 1 AGATCGG 37660 0.0 42.564785 1 CGCCGTA 26720 0.0 39.97528 46-47 AGAGCGT 40110 0.0 39.289116 9 GTCGCCG 27020 0.0 38.840744 44-45 CCGTATC 27700 0.0 38.7846 48-49 AAGAGCG 42210 0.0 38.38002 8 GATCGGA 42625 0.0 37.781967 2 ATCGGAA 43550 0.0 37.307014 3 CGGAAGA 44630 0.0 36.491646 5 TCGGAAG 44415 0.0 36.461266 4 TCCGATC 3260 0.0 35.84352 1 TCGGTGG 31290 0.0 34.42444 38-39 CGTGTTT 75940 0.0 33.807274 3 CCGATCT 3600 0.0 33.80205 2 TCTCGGT 31360 0.0 33.570957 36-37 TGGTCGC 31195 0.0 31.937496 42-43 CTCGTAT 3185 0.0 31.296738 1 >>END_MODULE