FastQCFastQC Report
Fri 27 May 2016
SRR1233085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42768573
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1328450.3106135900302308TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC720360.16843208680355082TruSeq Adapter, Index 8 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT661360.15463691061191123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT346050.077.763171
GCGTGTT502200.054.631092
AGATCGG360050.044.833131
GTTTTTT7203850.044.3632851
TATGCCG339300.038.37423346-47
GATCGGA451150.037.701672
TGCCGTC347200.036.4240948-49
ATCGGAA468400.036.3325843
CTCGTAT341200.036.22247742-43
CGTGTTT783450.035.551323
TCGGAAG478750.035.4885144
CGGAAGA487000.035.0853465
CCGTCTT372900.034.0869750-51
CGTATGC402500.032.58870744-45
CGTCTGA423550.032.2175316-17
ATCTCGT376400.032.03912440-41
CACACGT453350.030.16198712-13
CACGTCT456700.029.87365514-15
AGAGCAC635950.026.9828579
GAATCTC444850.026.78812838-39
Produced by FastQC (version 0.11.5)