##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42768573 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.799472898008545 31.0 30.0 34.0 23.0 34.0 2 30.736303172892864 33.0 31.0 34.0 27.0 34.0 3 30.913091699365324 34.0 31.0 34.0 27.0 34.0 4 34.29075847351746 37.0 35.0 37.0 31.0 37.0 5 34.13438358114029 37.0 35.0 37.0 30.0 37.0 6 34.128666579546625 37.0 35.0 37.0 30.0 37.0 7 34.20402635364991 37.0 35.0 37.0 31.0 37.0 8 34.16028488956131 37.0 35.0 37.0 31.0 37.0 9 35.75345331255265 39.0 37.0 39.0 31.0 39.0 10-11 35.64414261612142 39.0 37.0 39.0 30.0 39.0 12-13 35.58492828367221 39.0 37.0 39.0 30.0 39.0 14-15 36.84070628215723 40.0 38.0 41.0 30.0 41.0 16-17 36.608126520845104 40.0 37.5 41.0 30.0 41.0 18-19 36.15829274921097 40.0 36.5 41.0 28.5 41.0 20-21 35.70980632203931 40.0 35.5 41.0 27.0 41.0 22-23 35.353817954131884 40.0 35.0 41.0 24.5 41.0 24-25 34.88696398638318 40.0 35.0 41.0 17.5 41.0 26-27 34.33726391806432 40.0 35.0 41.0 7.5 41.0 28-29 33.85862290752604 40.0 35.0 41.0 2.0 41.0 30-31 33.42511108565628 40.0 34.0 41.0 2.0 41.0 32-33 33.22661258770546 40.0 34.0 41.0 2.0 41.0 34-35 33.2090438696657 40.0 34.0 41.0 2.0 41.0 36-37 33.11472715023716 40.0 34.0 41.0 2.0 41.0 38-39 33.002900763137454 40.0 34.0 41.0 2.0 41.0 40-41 32.78358606914475 40.0 33.0 41.0 2.0 41.0 42-43 32.762551207869386 40.0 33.0 41.0 2.0 41.0 44-45 32.67094172162349 40.0 33.0 41.0 2.0 41.0 46-47 32.55059356317547 40.0 33.0 41.0 2.0 41.0 48-49 32.4468334260299 39.5 33.0 41.0 2.0 41.0 50-51 32.31152428209377 39.0 33.0 41.0 2.0 41.0 52-53 32.14882545882464 39.0 32.5 41.0 2.0 41.0 54-55 31.998005673464952 39.0 32.0 41.0 2.0 41.0 56-57 31.851024267281492 39.0 32.0 41.0 2.0 41.0 58-59 31.774404736393706 39.0 32.0 41.0 2.0 41.0 60-61 31.671273518992557 39.0 32.0 41.0 2.0 41.0 62-63 31.48294970000519 38.0 31.5 41.0 2.0 41.0 64-65 31.06979852940148 38.0 31.0 40.0 2.0 41.0 66-67 30.897706570663466 37.5 31.0 40.0 2.0 41.0 68-69 30.54232999543847 37.0 30.0 40.0 2.0 41.0 70-71 30.2813280676912 36.5 30.0 40.0 2.0 41.0 72-73 29.975251524057164 36.0 29.0 39.0 2.0 41.0 74-75 29.670372553697312 35.5 29.0 39.0 2.0 41.0 76-77 28.590522858922604 34.5 28.0 37.5 2.0 39.0 78-79 28.99981811410916 35.0 29.0 37.0 2.0 39.5 80-81 28.828451278933247 35.0 29.0 37.0 2.0 39.0 82-83 28.524552093426173 35.0 29.0 37.0 2.0 39.0 84-85 28.22083289989591 35.0 29.0 36.0 2.0 38.5 86-87 27.911300384046015 35.0 28.5 36.0 2.0 37.0 88-89 27.63656275134548 35.0 27.5 35.5 2.0 37.0 90-91 27.379365205848696 35.0 27.0 35.0 2.0 36.5 92-93 27.14178359422934 34.5 26.0 35.0 2.0 36.0 94-95 26.91725481465094 34.0 26.0 35.0 2.0 36.0 96-97 26.679118894146878 34.0 25.5 35.0 2.0 36.0 98-99 26.41537033512902 34.0 25.0 35.0 2.0 35.5 100-101 25.397720997144326 33.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.221367708407989 1101 2 0.6267479323525791 1101 3 0.5470208727811219 1101 4 0.40870058652804175 1101 5 0.42165583084602076 1101 6 0.39456171090520087 1101 7 0.36343783277803254 1101 8 0.3895285602006382 1101 9 0.4956124812959928 1101 10-11 0.39227215965494366 1101 12-13 0.47653245785327414 1101 14-15 0.5785464031193968 1101 16-17 0.6053619661952041 1101 18-19 0.5510358261656023 1101 20-21 0.5084369745629047 1101 22-23 0.4984934038205253 1101 24-25 0.5281955679920713 1101 26-27 0.4902470016084379 1101 28-29 0.4481347359974066 1101 30-31 0.5776393649490146 1101 32-33 0.48694726375196495 1101 34-35 0.45262754186078524 1101 36-37 0.4336674533688907 1101 38-39 0.4361674740203014 1101 40-41 0.49962513297191435 1101 42-43 0.4459848510869051 1101 44-45 0.40733203847263866 1101 46-47 0.41177334337316296 1101 48-49 0.388369405399537 1101 50-51 0.3449500702820245 1101 52-53 0.3346980768145258 1101 54-55 0.33422366138652393 1101 56-57 0.3338064513175816 1101 58-59 0.38068401581182343 1101 60-61 0.3519363288843067 1101 62-63 0.37611734551240517 1101 64-65 0.21096710009458874 1101 66-67 0.28914722686772976 1101 68-69 0.21183684062864216 1101 70-71 0.2736630671565301 1101 72-73 0.29544647516804545 1101 74-75 0.3221900413680707 1101 76-77 0.3173960990886009 1101 78-79 0.3037811759679947 1101 80-81 0.27164768217922486 1101 82-83 0.30672136088173474 1101 84-85 0.2974425624770163 1101 86-87 0.365079070052186 1101 88-89 0.3160921418408442 1101 90-91 0.34416904438923623 1101 92-93 0.39201702526869653 1101 94-95 0.4465706515388099 1101 96-97 0.44557778782541035 1101 98-99 0.3917618607988693 1101 100-101 0.4730705329076237 1102 1 1.264881503941723 1102 2 0.6488367093045646 1102 3 0.564906136025197 1102 4 0.45648210358761077 1102 5 0.4723928191312723 1102 6 0.4771596986117075 1102 7 0.4388902563076371 1102 8 0.4352995455169548 1102 9 0.5018236088092252 1102 10-11 0.5411006994204968 1102 12-13 0.518845484575408 1102 14-15 0.6178504854257412 1102 16-17 0.6372593651343479 1102 18-19 0.6140780619241042 1102 20-21 0.49455589229322783 1102 22-23 0.519688671044733 1102 24-25 0.5552910836887932 1102 26-27 0.549944696031865 1102 28-29 0.5547180856046126 1102 30-31 0.6059130079728519 1102 32-33 0.49277410345393236 1102 34-35 0.45410287478903655 1102 36-37 0.46421926764316623 1102 38-39 0.47221696570166927 1102 40-41 0.5635206612315997 1102 42-43 0.42017941002122683 1102 44-45 0.43433419993076683 1102 46-47 0.4524664025862748 1102 48-49 0.4698763754545823 1102 50-51 0.47361918332324393 1102 52-53 0.4367274283785392 1102 54-55 0.4700958591152826 1102 56-57 0.419935192259576 1102 58-59 0.49281212753390236 1102 60-61 0.4299022318479615 1102 62-63 0.47192897783593324 1102 64-65 0.38505239653548173 1102 66-67 0.46856292574908665 1102 68-69 0.3756039031553371 1102 70-71 0.38430264162204253 1102 72-73 0.4247611545109038 1102 74-75 0.35120074496644804 1102 76-77 0.28403088288888867 1102 78-79 0.3955913486018616 1102 80-81 0.4379008882470501 1102 82-83 0.43129105787044963 1102 84-85 0.40482776384454766 1102 86-87 0.4008898873745501 1102 88-89 0.40053090344003905 1102 90-91 0.4214471994899327 1102 92-93 0.4062806485419195 1102 94-95 0.38695763554229856 1102 96-97 0.3790435186373138 1102 98-99 0.413785501598003 1102 100-101 0.4537225291315856 1103 1 1.2457032885873964 1103 2 0.675166422462226 1103 3 0.5858136458697025 1103 4 0.46118100724417843 1103 5 0.43188722376462607 1103 6 0.4299160034926004 1103 7 0.4146434921459772 1103 8 0.41912043712392233 1103 9 0.506319726557841 1103 10-11 0.531020565155174 1103 12-13 0.5441636254929705 1103 14-15 0.6634917630134325 1103 16-17 0.7083010115698869 1103 18-19 0.6541039875698615 1103 20-21 0.6419098311227103 1103 22-23 0.647783031878113 1103 24-25 0.6259327934953589 1103 26-27 0.6011153121269288 1103 28-29 0.533227917920037 1103 30-31 0.5888431883987906 1103 32-33 0.6195590066437191 1103 34-35 0.5451711570946074 1103 36-37 0.5562069624055184 1103 38-39 0.5754456900294684 1103 40-41 0.6934475007024119 1103 42-43 0.5762280778847995 1103 44-45 0.5602788984146017 1103 46-47 0.5500986610191632 1103 48-49 0.561079662617324 1103 50-51 0.5761686458974111 1103 52-53 0.5964186495989807 1103 54-55 0.5757219944448693 1103 56-57 0.5520045172544421 1103 58-59 0.5163432250817053 1103 60-61 0.5327212823039105 1103 62-63 0.5667164009244949 1103 64-65 0.5456996247203492 1103 66-67 0.586574630183712 1103 68-69 0.5630085830839029 1103 70-71 0.600005976538359 1103 72-73 0.5390507284684674 1103 74-75 0.5411728028759022 1103 76-77 0.4920163041338874 1103 78-79 0.5540486769267048 1103 80-81 0.5238909394469893 1103 82-83 0.5397650884257068 1103 84-85 0.5173698297156619 1103 86-87 0.521655829214172 1103 88-89 0.5093863369315237 1103 90-91 0.5091245661036474 1103 92-93 0.4658979784489965 1103 94-95 0.4712061867568984 1103 96-97 0.5313097144690637 1103 98-99 0.5424178634443919 1103 100-101 0.5682261022995085 1104 1 1.202440858260566 1104 2 0.5888787981698691 1104 3 0.4980970356609298 1104 4 0.4200615070116882 1104 5 0.4131392084056529 1104 6 0.39370241199780764 1104 7 0.3681441361982465 1104 8 0.3771972183567769 1104 9 0.4805252802250095 1104 10-11 0.44785221070107895 1104 12-13 0.4872340305527274 1104 14-15 0.5350893763262476 1104 16-17 0.5462900618869853 1104 18-19 0.4968411658569991 1104 20-21 0.4470430613880225 1104 22-23 0.4961186169506675 1104 24-25 0.5391666206011578 1104 26-27 0.49628997693638865 1104 28-29 0.49735797941349347 1104 30-31 0.554332508869912 1104 32-33 0.5181980919078555 1104 34-35 0.5144410872655172 1104 36-37 0.5230700472689591 1104 38-39 0.5176231309916872 1104 40-41 0.6079191667770161 1104 42-43 0.5196508608617947 1104 44-45 0.5188504057500367 1104 46-47 0.5291270662057102 1104 48-49 0.41050690107639554 1104 50-51 0.48442991862643225 1104 52-53 0.5086373883303352 1104 54-55 0.5003122309291328 1104 56-57 0.5086584192759211 1104 58-59 0.5336475898955939 1104 60-61 0.5274436057510599 1104 62-63 0.5494975255773404 1104 64-65 0.5380458061006017 1104 66-67 0.5551893529433904 1104 68-69 0.4882163305165115 1104 70-71 0.4886162681208326 1104 72-73 0.5155260495809983 1104 74-75 0.4674916532135107 1104 76-77 0.400044490567808 1104 78-79 0.5219450791550777 1104 80-81 0.5149275711806034 1104 82-83 0.4812988280699493 1104 84-85 0.4593838876087908 1104 86-87 0.4779568397684706 1104 88-89 0.4986822636916486 1104 90-91 0.528546512782853 1104 92-93 0.4384151655377728 1104 94-95 0.49269353442659636 1104 96-97 0.4777426260649058 1104 98-99 0.48574008311204864 1104 100-101 0.5590667944043766 1105 1 1.1774641629544327 1105 2 0.5709670694309068 1105 3 0.5143545496077522 1105 4 0.3977363163274532 1105 5 0.38684334011667687 1105 6 0.3536244963288979 1105 7 0.35409274568363713 1105 8 0.3258660470620143 1105 9 0.3631584675546833 1105 10-11 0.38414334681009876 1105 12-13 0.4277918602920465 1105 14-15 0.5243623623666096 1105 16-17 0.5248478954865021 1105 18-19 0.5035378094347465 1105 20-21 0.5015695011059833 1105 22-23 0.4867465365496315 1105 24-25 0.46854073942901664 1105 26-27 0.44882452098485004 1105 28-29 0.44892991974955265 1105 30-31 0.48723925643066224 1105 32-33 0.4979134402862755 1105 34-35 0.40883308590822054 1105 36-37 0.4040297928254688 1105 38-39 0.43428399636599124 1105 40-41 0.49714720184122285 1105 42-43 0.44424677467402773 1105 44-45 0.41144361686206565 1105 46-47 0.4468679807719269 1105 48-49 0.4480275088845005 1105 50-51 0.45702352887450104 1105 52-53 0.42330198979376377 1105 54-55 0.39224102933616933 1105 56-57 0.4468729170195651 1105 58-59 0.45793721677380006 1105 60-61 0.42743124828879075 1105 62-63 0.42985541996809573 1105 64-65 0.4070438228087099 1105 66-67 0.40488853924706447 1105 68-69 0.37362213121038224 1105 70-71 0.4025510510339849 1105 72-73 0.4275202553907711 1105 74-75 0.43941725120829034 1105 76-77 0.33031305531062927 1105 78-79 0.3995080121547794 1105 80-81 0.4462712353342049 1105 82-83 0.43814487410733705 1105 84-85 0.45185945089946244 1105 86-87 0.4419586021145179 1105 88-89 0.49032752002353774 1105 90-91 0.5118654150897086 1105 92-93 0.5250821739373457 1105 94-95 0.564503276329404 1105 96-97 0.540887158916231 1105 98-99 0.6322523326628442 1105 100-101 0.6311906800774985 1106 1 1.123351249542381 1106 2 0.5207821585715848 1106 3 0.5030974527042318 1106 4 0.4720970846856076 1106 5 0.304836551714331 1106 6 0.2635337037537937 1106 7 0.3210746043843997 1106 8 0.3297254583204605 1106 9 0.35524423082641476 1106 10-11 0.37857614953235696 1106 12-13 0.39657517352866023 1106 14-15 0.4655159290884541 1106 16-17 0.5123680337429448 1106 18-19 0.5007678032802971 1106 20-21 0.5002329050068468 1106 22-23 0.5155224829854461 1106 24-25 0.43580097600767687 1106 26-27 0.450002149413649 1106 28-29 0.42904710407317026 1106 30-31 0.49392572970189264 1106 32-33 0.5029202488038962 1106 34-35 0.4635227883766504 1106 36-37 0.49173540803406723 1106 38-39 0.5122435640136231 1106 40-41 0.6098794253547339 1106 42-43 0.5116279182407553 1106 44-45 0.4932293162403809 1106 46-47 0.4909067263574869 1106 48-49 0.5020448574552887 1106 50-51 0.5295727591681612 1106 52-53 0.5343167126689323 1106 54-55 0.5677288635049322 1106 56-57 0.5909578837399927 1106 58-59 0.634608275578735 1106 60-61 0.6024367925036529 1106 62-63 0.611295146445844 1106 64-65 0.5690581167736255 1106 66-67 0.6475589422300025 1106 68-69 0.563977135966816 1106 70-71 0.6064094409953711 1106 72-73 0.577890498658725 1106 74-75 0.580375230266533 1106 76-77 0.4395418372687807 1106 78-79 0.56363103213614 1106 80-81 0.5566757947011318 1106 82-83 0.5790428179848064 1106 84-85 0.588943842647339 1106 86-87 0.5417727575471964 1106 88-89 0.5844678331054922 1106 90-91 0.5852198218329185 1106 92-93 0.5908926126771554 1106 94-95 0.5965479601597785 1106 96-97 0.6323995348662841 1106 98-99 0.6487383950041803 1106 100-101 0.6058475624508155 1107 1 1.1090906444013235 1107 2 0.5269713859640639 1107 3 0.4626548132583359 1107 4 0.3695145245398095 1107 5 0.32812505850594675 1107 6 0.3271926862161152 1107 7 0.2849998571613952 1107 8 0.31999193942950654 1107 9 0.37562943044544284 1107 10-11 0.3577018239985108 1107 12-13 0.3779448579499558 1107 14-15 0.411688364962707 1107 16-17 0.440266647899314 1107 18-19 0.41484372312802975 1107 20-21 0.35899260307316183 1107 22-23 0.3886646867703547 1107 24-25 0.31573510963580986 1107 26-27 0.3161169741339478 1107 28-29 0.3453788897518777 1107 30-31 0.3014701414012748 1107 32-33 0.30819639838751556 1107 34-35 0.2591756816778812 1107 36-37 0.26061638447541924 1107 38-39 0.2274000445252966 1107 40-41 0.2735889426814353 1107 42-43 0.26371768849880795 1107 44-45 0.29603260661008335 1107 46-47 0.26630453483380023 1107 48-49 0.3182956152568579 1107 50-51 0.2285242081486416 1107 52-53 0.1996725022896797 1107 54-55 0.16513058373340783 1107 56-57 0.19747515646975344 1107 58-59 0.25325377455797593 1107 60-61 0.19548021538358284 1107 62-63 0.2564695351350288 1107 64-65 0.2384576264434699 1107 66-67 0.26102989618497574 1107 68-69 0.2554704528544214 1107 70-71 0.290795671979712 1107 72-73 0.2937787661516609 1107 74-75 0.2769471852730163 1107 76-77 0.11209453050922136 1107 78-79 0.21357308843890266 1107 80-81 0.24091646391070398 1107 82-83 0.3067402124248524 1107 84-85 0.261866020483275 1107 86-87 0.26987541688725614 1107 88-89 0.25670713097322917 1107 90-91 0.22823898104676843 1107 92-93 0.2556957504353612 1107 94-95 0.2512806322821497 1107 96-97 0.2520379447046892 1107 98-99 0.2316437279085619 1107 100-101 0.2772355923973997 1108 1 1.0925300947337533 1108 2 0.5336011867150461 1108 3 0.4301888065148276 1108 4 0.3963433147068969 1108 5 0.30002894151594717 1108 6 0.2793666614870247 1108 7 0.2755895859085058 1108 8 0.256239241230233 1108 9 0.30449441568291036 1108 10-11 0.3078274842397022 1108 12-13 0.3386848239098441 1108 14-15 0.338253615497095 1108 16-17 0.2945972223419133 1108 18-19 0.2725532088649629 1108 20-21 0.2799836985973414 1108 22-23 0.23661838927863954 1108 24-25 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-3.8964330517449675 2316 50-51 -3.857300752587971 2316 52-53 -3.8366185210983588 2316 54-55 -3.7982569066207503 2316 56-57 -3.835714705300081 2316 58-59 -3.9008717261193837 2316 60-61 -3.8683030043057727 2316 62-63 -3.8404831794919296 2316 64-65 -3.7394050632906186 2316 66-67 -3.7908459881029763 2316 68-69 -3.690883500357746 2316 70-71 -3.6519272829279217 2316 72-73 -3.624459088572273 2316 74-75 -3.65551482279313 2316 76-77 -3.5274194325844412 2316 78-79 -3.6654787926883863 2316 80-81 -3.681390411406664 2316 82-83 -3.698087160081517 2316 84-85 -3.6447064250835446 2316 86-87 -3.5962987084094102 2316 88-89 -3.6055446208559694 2316 90-91 -3.6498276930118045 2316 92-93 -3.6561411108381385 2316 94-95 -3.6130958015952075 2316 96-97 -3.61995133151083 2316 98-99 -3.569686869025219 2316 100-101 -3.4175359097066575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1364544.0 3 270304.0 4 174645.0 5 184781.0 6 457869.0 7 436941.0 8 353945.0 9 563405.0 10 828410.0 11 719628.0 12 477980.0 13 325655.0 14 271066.0 15 243684.0 16 217227.0 17 193206.0 18 177736.0 19 170182.0 20 167984.0 21 175764.0 22 192511.0 23 218177.0 24 249540.0 25 282877.0 26 328293.0 27 389732.0 28 423884.0 29 471957.0 30 561876.0 31 683347.0 32 845847.0 33 1086333.0 34 1455782.0 35 2097594.0 36 3315878.0 37 6394605.0 38 1.0951075E7 39 5023007.0 40 21302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.577554186367355 17.018186755497286 10.364520197548254 45.03973886058711 2 12.776648573943541 16.144259590903236 59.61147349455502 11.467618340598202 3 17.884790191222727 21.00883221513044 40.369759655943035 20.73661793770379 4 19.565966294173844 28.08120876820908 36.71118013228405 15.641644805333021 5 19.4564107299817 30.664140191800826 36.5184127510306 13.361036327186868 6 15.531065823358317 33.496259467443565 37.75010848645841 13.222566222739712 7 12.650703721274908 18.476438072338162 55.048797527505464 13.824060678881464 8 12.717011004663714 21.204270496431445 48.869957795841565 17.208760703063277 9 14.167303070111373 20.161783301501064 48.366049863839244 17.304863764548315 10-11 17.711202195361256 30.454118915260842 36.8150559799533 15.019622909424605 12-13 16.470929309452515 24.587849065311502 42.715456070920986 16.225765554315 14-15 16.70662056229162 26.303445856773433 40.961963445596105 16.027970135338848 16-17 17.810359320973763 26.39822201264651 39.31533686249063 16.476081803889087 18-19 18.18086585460414 27.075722445405642 38.439572989508505 16.303838710481706 20-21 18.35340858293704 27.095148579949914 37.391602511873124 17.159840325239927 22-23 18.779013506531278 28.64055865258538 36.05754796961801 16.522879871265342 24-25 18.452455398464615 28.754537162574884 35.49155256775484 17.301454871205664 26-27 18.468947729480277 28.728446254401675 34.827370181962955 17.975235834155086 28-29 19.02585404353203 29.875674979946915 33.27119346561673 17.82727751090432 30-31 18.98553845256079 29.541186782699697 33.362644687598056 18.110630077141458 32-33 18.90960940035481 30.324754193869698 32.17300824977554 18.59262815599995 34-35 19.08637206352214 30.470847955095742 31.95826829322034 18.48451168816178 36-37 19.034472974494577 29.924762723824337 32.75841694120661 18.282347360474475 38-39 19.638346898871813 29.685417200209052 32.564158244752115 18.11207765616702 40-41 19.183958556186898 29.904399952616252 32.92151627817975 17.990125213017105 42-43 19.26681601556949 29.588259336568505 32.91439362709254 18.230531020769465 44-45 19.630018903959595 28.960588231052615 33.03392459672637 18.375468268261415 46-47 19.12897419714231 29.405968774734692 32.997725345957484 18.46733168216551 48-49 19.1933927820994 29.67655399571284 32.734578885779484 18.39547433640827 50-51 18.867189026975723 29.721236117936336 32.421269846014184 18.99030500907376 52-53 19.390158878761106 30.21228292179523 31.67015513791939 18.72740306152427 54-55 18.956934291032553 30.309794228318452 31.45011499273249 19.283156487916504 56-57 19.312556462964377 30.209548696736544 31.443270036668043 19.034624803631036 58-59 19.296227691490557 30.294960749701705 31.252965621855033 19.15584593695271 60-61 19.60846085647785 30.70711929603471 30.37125353524011 19.313166312247333 62-63 19.568864154613095 30.528378780644367 31.216441233902653 18.686315830839884 64-65 20.130153477558288 30.82209158314903 30.3540961493909 18.69365878990178 66-67 19.72313937086489 31.557781711394 29.96367277375323 18.755406143987884 68-69 19.952929070562003 31.27532652847849 30.259138156098135 18.512606244861367 70-71 19.877521268897333 31.222220423016832 30.116994792040856 18.78326351604498 72-73 19.47025161919879 31.650883337776996 30.0131906895536 18.865674353470613 74-75 19.384732886282244 31.057821033133852 30.761254525110477 18.79619155547342 76-77 19.31264425456352 31.191992941993174 30.62116120304213 18.87420160040118 78-79 19.411510050970215 31.2778340694014 30.16924428464109 19.141411594987296 80-81 19.416296140901583 30.84075393434954 30.503157881073818 19.23979204367506 82-83 19.524786239069417 31.121160752836634 30.155225189361147 19.1988278187328 84-85 19.456143760446654 30.874172190076877 30.442845713326182 19.22683833615029 86-87 19.68066592102895 31.292137323141738 29.903978262249097 19.123218493580215 88-89 19.501840324811933 31.186410118496894 30.11824032246374 19.193509234227434 90-91 19.61019751187468 31.193656534767065 30.11701988622089 19.079126067137363 92-93 19.641241845095394 31.144085281988787 29.86647332884515 19.34819954407067 94-95 19.301733074180348 31.036295837017512 30.149416891167334 19.512554197634802 96-97 19.349364250150487 31.53755651561967 29.747112073586923 19.36596716064292 98-99 19.257966298852175 31.447788823529898 29.919049599760267 19.375195277857657 100-101 19.616771494843764 31.775242229393324 29.167165908789254 19.440820366973657 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17671.0 1 21823.0 2 23225.5 3 25192.5 4 35367.0 5 47746.5 6 60027.5 7 72619.5 8 87170.5 9 101715.0 10 116469.0 11 130926.5 12 144767.0 13 157532.5 14 169740.5 15 181466.5 16 192576.0 17 206636.0 18 229161.0 19 256240.0 20 284386.0 21 331512.5 22 394160.5 23 464349.0 24 561604.0 25 668850.5 26 781582.5 27 902390.5 28 1083885.5 29 1243839.0 30 1370682.5 31 1510055.5 32 1580786.0 33 1612189.5 34 1584599.5 35 1597444.0 36 1671354.5 37 1678125.0 38 1626422.5 39 1521954.0 40 1430555.5 41 1379711.0 42 1314503.0 43 1236849.5 44 1136642.5 45 1061693.5 46 1034661.0 47 1002982.0 48 966037.0 49 949200.5 50 885473.0 51 781394.5 52 705751.0 53 653352.5 54 588847.5 55 511638.0 56 455426.0 57 396076.0 58 336619.5 59 281660.5 60 220783.5 61 166113.5 62 127507.0 63 96133.5 64 67324.0 65 47468.5 66 34961.0 67 27500.0 68 21679.5 69 16562.5 70 13847.0 71 13132.0 72 11752.0 73 12484.0 74 11564.5 75 7203.0 76 5324.5 77 4896.0 78 4106.0 79 2626.5 80 1934.5 81 1452.0 82 1103.5 83 812.0 84 587.5 85 403.5 86 290.5 87 211.5 88 128.0 89 83.5 90 50.5 91 24.5 92 19.0 93 12.5 94 4.0 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050857436838025905 2 0.006885897268538747 3 0.006151713315288775 4 0.0038907073191336073 5 0.03861480250930982 6 0.11985436128532977 7 0.1349121468233228 8 0.14357972616949366 9 0.14598336025847763 10-11 0.15684764605075788 12-13 0.16264629638215894 14-15 0.1655830321951588 16-17 0.1751426216628738 18-19 0.17330131636610838 20-21 0.17632924063190045 22-23 0.1488896999205468 24-25 0.16901429000214713 26-27 0.17476968427260828 28-29 0.17685415877681962 30-31 0.19050086146198986 32-33 0.1994233008428876 34-35 0.19719502916311937 36-37 0.19068791469848667 38-39 0.21953970734539122 40-41 0.2435409757533879 42-43 0.25424042088100535 44-45 0.2869992880052369 46-47 0.2710705358348056 48-49 0.2654390643335236 50-51 0.2600870036042587 52-53 0.2565540355999252 54-55 0.25772428741075837 56-57 0.27291301021429915 58-59 0.2644488512628186 60-61 0.2721940243365146 62-63 0.28051789336062255 64-65 0.3766924839881845 66-67 0.1827054178309854 68-69 0.3157470322893401 70-71 0.006501269050992186 72-73 0.0038942145673179227 74-75 0.01518755372081271 76-77 0.033419398865610975 78-79 0.01666878153732181 80-81 0.02355818605404487 82-83 0.028165541085506875 84-85 0.022593692803358205 86-87 0.019424309527465412 88-89 0.02867058482404826 90-91 0.020551305277358684 92-93 0.020753556589320855 94-95 0.030705957853679147 96-97 0.03278341786152183 98-99 0.017275535473208332 100-101 0.028287125689229798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.2768573E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.145648939888307 #Duplication Level Percentage of deduplicated Percentage of total 1 77.95832388525396 22.721459399017213 2 8.744367749847392 5.097205452766662 3 3.46759249109738 3.03195700236351 4 1.9895511978867748 2.3194704304616875 5 1.2881685825847355 1.877225464170411 6 0.8723003492217191 1.525425584913492 7 0.6700721936275817 1.3670782243895254 8 0.4968788030065483 1.158548412648062 9 0.4385078760516015 1.1502536951498432 >10 3.3321175272498844 19.36795177854516 >50 0.40921078410894507 8.334049326346104 >100 0.2944474127067271 16.484879980466385 >500 0.0249194237265349 4.909490878663012 >1k 0.01237495389200757 6.740751582478427 >5k 8.183593501629128E-4 1.5540601733864925 >10k+ 3.484103879380767E-4 2.360192614233984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG 132845 0.3106135900302308 TruSeq Adapter, Index 8 (100% over 49bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC 72036 0.16843208680355082 TruSeq Adapter, Index 8 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66136 0.15463691061191123 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.38489944473948196 0.0 0.0 9.352661824840404E-6 0.0 2 0.38636079814961327 0.0 0.0 1.8705323649680808E-5 0.0 3 0.386669435989833 0.0 0.0 1.8705323649680808E-5 0.0 4 0.3883225189673735 2.338165456210101E-6 0.0 2.104348910589091E-5 0.0 5 0.3887714467349659 2.338165456210101E-6 0.0 2.104348910589091E-5 0.0 6 0.38929285763170074 2.338165456210101E-6 0.0 2.104348910589091E-5 0.0 7 0.38968800759380023 2.338165456210101E-6 0.0 2.338165456210101E-5 0.0 8 0.39137850121864015 2.338165456210101E-6 0.0 3.039615093073131E-5 0.0 9 0.39183911981351355 7.0144963686303025E-6 0.0 3.507248184315151E-5 0.0 10-11 0.3936944541030163 7.0144963686303025E-6 0.0 4.676330912420202E-5 1.1690827281050505E-6 12-13 0.3965949483514449 7.0144963686303025E-6 0.0 7.131404641440807E-5 3.5072481843151517E-6 14-15 0.399044176666825 7.0144963686303025E-6 0.0 2.0926580833080402E-4 4.676330912420202E-6 16-17 0.40198441972800913 7.0144963686303025E-6 0.0 3.2500499841320405E-4 4.676330912420202E-6 18-19 0.4048369815845855 7.0144963686303025E-6 0.0 3.6124656298446055E-4 4.676330912420202E-6 20-21 0.40906204656395717 9.352661824840404E-6 0.0 3.822900520903515E-4 4.676330912420202E-6 22-23 0.4123647052708539 9.352661824840404E-6 0.0 4.126862030210828E-4 4.676330912420202E-6 24-25 0.41512841684009427 9.352661824840404E-6 0.0 4.1853161666160804E-4 4.676330912420202E-6 26-27 0.41733681411348467 9.352661824840404E-6 0.0 4.1853161666160804E-4 4.676330912420202E-6 28-29 0.41935465090219404 9.352661824840404E-6 0.0 4.243770303021333E-4 4.676330912420202E-6 30-31 0.4214777051364328 9.352661824840404E-6 0.0 4.2554611303023833E-4 4.676330912420202E-6 32-33 0.42338214090051585 1.0521744552945454E-5 0.0 4.2554611303023833E-4 4.676330912420202E-6 34-35 0.42605817126514833 1.4028992737260605E-5 0.0 4.2554611303023833E-4 4.676330912420202E-6 36-37 0.4279275345473883 1.4028992737260605E-5 0.0 4.2788427848644843E-4 4.676330912420202E-6 38-39 0.42986353554513035 1.4028992737260605E-5 0.0 4.3022244394265853E-4 4.676330912420202E-6 40-41 0.4318895559129363 1.4028992737260605E-5 0.0 4.325606093988686E-4 4.676330912420202E-6 42-43 0.43390622361891756 1.4028992737260605E-5 0.0 4.348987748550787E-4 4.676330912420202E-6 44-45 0.436159046035976 1.4028992737260605E-5 0.0 4.4658960213612926E-4 4.676330912420202E-6 46-47 0.438362766978454 1.4028992737260605E-5 0.0 4.5126593304854946E-4 4.676330912420202E-6 48-49 0.4406681981182772 1.4028992737260605E-5 0.0 4.5828042941717975E-4 4.676330912420202E-6 50-51 0.4431980931418965 1.6367158193470707E-5 0.0 4.6061859487338985E-4 4.676330912420202E-6 52-53 0.445874123506529 1.6367158193470707E-5 0.0 4.6529492578581004E-4 4.676330912420202E-6 54-55 0.4487325307767458 1.6367158193470707E-5 0.0 4.886765803479111E-4 4.676330912420202E-6 56-57 0.4519440010308504 1.6367158193470707E-5 0.0 5.085509867256969E-4 4.676330912420202E-6 58-59 0.45548164536609625 1.6367158193470707E-5 0.0 5.249181449191677E-4 4.676330912420202E-6 60-61 0.45947172471711883 1.6367158193470707E-5 0.0 5.459616340250586E-4 4.676330912420202E-6 62-63 0.46383591054113493 1.6367158193470707E-5 0.0 5.56483378578004E-4 4.676330912420202E-6 64-65 0.46856601925904795 1.7536240921575756E-5 0.0 5.646669576747393E-4 4.676330912420202E-6 66-67 0.47381286254278343 1.8705323649680808E-5 2.338165456210101E-6 5.775268676838949E-4 4.676330912420202E-6 68-69 0.4799996483399154 1.8705323649680808E-5 2.338165456210101E-6 5.822031985963151E-4 7.0144963686303025E-6 70-71 0.48658742951278733 1.8705323649680808E-5 2.338165456210101E-6 5.868795295087353E-4 7.0144963686303025E-6 72-73 0.49408826429630937 1.8705323649680808E-5 4.676330912420202E-6 5.974012740616807E-4 7.0144963686303025E-6 74-75 0.5027359692361024 1.8705323649680808E-5 4.676330912420202E-6 6.079230186146262E-4 8.183579096735354E-6 76-77 0.5125504187385443 1.8705323649680808E-5 4.676330912420202E-6 6.72222568660404E-4 1.0521744552945454E-5 78-79 0.5237268496192286 1.8705323649680808E-5 4.676330912420202E-6 7.18985877784606E-4 1.1690827281050504E-5 80-81 0.5363494358345788 1.8705323649680808E-5 7.0144963686303025E-6 7.365221187061817E-4 1.1690827281050504E-5 82-83 0.5505947088765388 1.8705323649680808E-5 7.0144963686303025E-6 8.125124960330101E-4 1.1690827281050504E-5 84-85 0.5676563022105039 2.2212571833995957E-5 7.0144963686303025E-6 8.768120460787878E-4 1.1690827281050504E-5 86-87 0.5871986890935079 2.338165456210101E-5 7.0144963686303025E-6 9.095463624657292E-4 1.1690827281050504E-5 88-89 0.6097362659259171 2.338165456210101E-5 7.0144963686303025E-6 9.142226933781494E-4 1.1690827281050504E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 34605 0.0 77.76317 1 GCGTGTT 50220 0.0 54.63109 2 AGATCGG 36005 0.0 44.83313 1 GTTTTTT 720385 0.0 44.363285 1 TATGCCG 33930 0.0 38.374233 46-47 GATCGGA 45115 0.0 37.70167 2 TGCCGTC 34720 0.0 36.42409 48-49 ATCGGAA 46840 0.0 36.332584 3 CTCGTAT 34120 0.0 36.222477 42-43 CGTGTTT 78345 0.0 35.55132 3 TCGGAAG 47875 0.0 35.488514 4 CGGAAGA 48700 0.0 35.085346 5 CCGTCTT 37290 0.0 34.08697 50-51 CGTATGC 40250 0.0 32.588707 44-45 CGTCTGA 42355 0.0 32.21753 16-17 ATCTCGT 37640 0.0 32.039124 40-41 CACACGT 45335 0.0 30.161987 12-13 CACGTCT 45670 0.0 29.873655 14-15 AGAGCAC 63595 0.0 26.982857 9 GAATCTC 44485 0.0 26.788128 38-39 >>END_MODULE