FastQCFastQC Report
Fri 27 May 2016
SRR1233084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39906606
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1091800.27358878878349113Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT776360.19454423159914927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC122100.068.922649
CGCGTGT212950.065.4982151
AAGACGG143000.059.2807358
GTTTTTT7269550.050.3282621
GCGTGTT309800.045.156782
GAAGACG207300.040.9839367
CGGCATA106650.039.1660112-13
GACGGCA117750.035.8350210-11
TACGAGA116600.035.45709216-17
AGATCGG377650.034.7986071
ACGGCAT122150.034.09758410-11
ATACGAG125450.033.50405516-17
GATCGGA442300.030.0707932
CGAGATT138400.029.70314818-19
ATCGGAA448850.029.6751213
CATACGA144600.029.13258214-15
TCGGAAG461350.028.736784
ACGAGAT148650.028.6922618-19
CGCCGTA332000.028.50357246-47
CGGAAGA468200.028.3230115
Produced by FastQC (version 0.11.5)