##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39906606 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772688486713204 34.0 31.0 34.0 30.0 34.0 2 32.145208414867454 34.0 31.0 34.0 30.0 34.0 3 32.20299438644319 34.0 31.0 34.0 30.0 34.0 4 35.487345954702334 37.0 37.0 37.0 33.0 37.0 5 35.45402099592233 37.0 37.0 37.0 33.0 37.0 6 35.51744012006433 37.0 37.0 37.0 35.0 37.0 7 35.55961709697888 37.0 37.0 37.0 35.0 37.0 8 35.561365779891176 37.0 37.0 37.0 35.0 37.0 9 37.28424399208492 39.0 38.0 39.0 35.0 39.0 10-11 37.23039904721539 39.0 38.0 39.0 35.0 39.0 12-13 37.17629675147017 39.0 38.0 39.0 34.5 39.0 14-15 38.56959427970396 41.0 39.0 41.0 34.5 41.0 16-17 38.33874525686299 40.5 39.0 41.0 34.0 41.0 18-19 37.79515328865602 40.0 37.0 41.0 33.0 41.0 20-21 37.39418457435343 40.0 37.0 41.0 32.5 41.0 22-23 36.89803876080066 40.0 37.0 41.0 31.5 41.0 24-25 36.29563273308685 40.0 36.5 41.0 28.0 41.0 26-27 35.85947955584095 40.0 37.0 41.0 24.0 41.0 28-29 35.41008917170255 40.0 37.0 41.0 17.0 41.0 30-31 35.04824966823789 40.0 36.5 41.0 11.0 41.0 32-33 34.83162067202608 40.0 36.0 41.0 8.5 41.0 34-35 34.635680744185564 40.0 36.0 41.0 7.0 41.0 36-37 34.49481777528262 40.0 36.0 41.0 4.0 41.0 38-39 34.294035190564685 40.0 35.5 41.0 2.0 41.0 40-41 34.18901389409062 40.0 35.0 41.0 2.0 41.0 42-43 34.07864482386701 40.0 35.0 41.0 2.0 41.0 44-45 33.80298442318046 40.0 35.0 41.0 2.0 41.0 46-47 33.730218901602406 40.0 35.0 41.0 2.0 41.0 48-49 33.66682570048678 40.0 35.0 41.0 2.0 41.0 50-51 33.15259769272285 39.0 34.0 40.5 2.0 41.0 52-53 33.156418025125966 39.5 34.5 40.5 2.0 41.0 54-55 33.394720876538585 40.0 35.0 41.0 2.0 41.0 56-57 33.278417362779486 40.0 35.0 41.0 2.0 41.0 58-59 33.11296450517491 40.0 34.0 41.0 2.0 41.0 60-61 33.06277126147987 39.5 34.0 41.0 2.0 41.0 62-63 32.87415482038237 39.0 34.0 41.0 2.0 41.0 64-65 32.60759867677046 39.0 34.0 41.0 2.0 41.0 66-67 32.27188126447035 38.5 33.5 41.0 2.0 41.0 68-69 31.96126276687123 38.0 33.0 40.5 2.0 41.0 70-71 31.619876405926377 37.0 33.0 40.0 2.0 41.0 72-73 31.196210359257314 37.0 32.0 39.5 2.0 41.0 74-75 30.877676041906447 36.0 32.0 39.0 2.0 41.0 76-77 30.533095861371926 36.0 31.5 39.0 2.0 41.0 78-79 30.159444629292704 35.0 31.0 38.5 2.0 40.0 80-81 29.781336929529914 35.0 31.0 37.0 2.0 39.0 82-83 29.409414559083274 35.0 31.0 37.0 2.0 39.0 84-85 29.04457127975253 35.0 30.0 36.0 2.0 38.5 86-87 28.688880795826133 35.0 30.0 36.0 2.0 37.0 88-89 28.378935570216118 35.0 29.5 36.0 2.0 37.0 90-91 28.111037192689352 35.0 29.0 35.0 2.0 36.5 92-93 27.82965745069876 35.0 29.0 35.0 2.0 36.0 94-95 27.537106751198035 35.0 29.0 35.0 2.0 36.0 96-97 27.24531607624061 34.0 27.5 35.0 2.0 36.0 98-99 26.9353021276728 34.0 27.0 35.0 2.0 35.5 100-101 25.227056818612937 32.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3016454984166401 1101 2 0.2382924056931799 1101 3 0.25541038636765734 1101 4 0.2844850457562771 1101 5 0.2947939011059191 1101 6 0.2651338040765978 1101 7 0.27292578591805494 1101 8 0.23937681284817813 1101 9 0.24428887588027948 1101 10-11 0.22835368596759054 1101 12-13 0.23981497668250995 1101 14-15 0.2505237311104125 1101 16-17 0.27687335097638055 1101 18-19 0.23867182452982405 1101 20-21 0.2905650358462353 1101 22-23 0.33817130522204764 1101 24-25 0.3318870844010604 1101 26-27 0.27270092682783087 1101 28-29 0.3402412025134893 1101 30-31 0.23491808500765643 1101 32-33 0.1809938972420042 1101 34-35 0.14791569545132432 1101 36-37 0.14793876311318144 1101 38-39 0.22663708649673708 1101 40-41 0.25945164847843927 1101 42-43 0.22045305817100314 1101 44-45 0.13424705408414894 1101 46-47 0.1090993358886223 1101 48-49 0.16353850402870762 1101 50-51 0.15853583346956412 1101 52-53 0.11591250732539038 1101 54-55 0.20230841472056937 1101 56-57 0.17333884872299166 1101 58-59 0.1668431754518096 1101 60-61 0.2713943312799785 1101 62-63 0.26735513092622654 1101 64-65 0.2811105604958186 1101 66-67 0.24862420650262607 1101 68-69 0.2397259122673816 1101 70-71 0.23985733500682827 1101 72-73 0.25512012725813804 1101 74-75 0.23300207988174293 1101 76-77 0.22273717681128957 1101 78-79 0.24192307282843473 1101 80-81 0.2715085903401331 1101 82-83 0.2844067954841556 1101 84-85 0.2197137245770584 1101 86-87 0.2363500252253239 1101 88-89 0.18373365693855348 1101 90-91 0.16839594633784216 1101 92-93 0.15132767005813363 1101 94-95 0.15306044983904243 1101 96-97 0.16952624165260488 1101 98-99 0.1631470464298097 1101 100-101 0.49506145215129393 1102 1 0.1661821686906393 1102 2 0.17855400902973173 1102 3 0.13912837440727088 1102 4 0.17337482338962218 1102 5 0.2198660705010056 1102 6 0.18189998085693304 1102 7 0.13575322616073038 1102 8 0.1470285341166715 1102 9 0.14878408689590117 1102 10-11 0.16427837012537339 1102 12-13 0.2012596844611707 1102 14-15 0.20852365167646525 1102 16-17 0.2183714927835112 1102 18-19 0.2713734970137551 1102 20-21 0.2950609737532446 1102 22-23 0.31754824804868775 1102 24-25 0.31216808335122437 1102 26-27 0.25824754583699416 1102 28-29 0.2349217727613535 1102 30-31 0.2364228534102395 1102 32-33 0.26124671044900794 1102 34-35 0.226493366420506 1102 36-37 0.1807464404647945 1102 38-39 0.24478216885826498 1102 40-41 0.22166400559660104 1102 42-43 0.17234685184577359 1102 44-45 0.10485947119622097 1102 46-47 0.04145793178533097 1102 48-49 0.1285749774192695 1102 50-51 0.175029426892074 1102 52-53 0.16725094036021915 1102 54-55 0.22807889325742536 1102 56-57 0.19605098124412734 1102 58-59 0.14259490476904801 1102 60-61 0.25964649459336187 1102 62-63 0.2814836466178221 1102 64-65 0.30544702713098815 1102 66-67 0.35170775619375405 1102 68-69 0.29938518007629966 1102 70-71 0.28597221238458914 1102 72-73 0.3420176087868967 1102 74-75 0.3377324922161087 1102 76-77 0.2925573392112142 1102 78-79 0.28858866223017543 1102 80-81 0.24355736525835425 1102 82-83 0.19035109061864475 1102 84-85 0.19700849417230515 1102 86-87 0.2775880776441433 1102 88-89 0.2882849814252815 1102 90-91 0.31067840898459664 1102 92-93 0.2743132771446497 1102 94-95 0.22471594825216457 1102 96-97 0.1853971271326138 1102 98-99 0.24412476963644636 1102 100-101 0.5173288295239438 1103 1 0.1865699573756281 1103 2 0.09675972743510641 1103 3 0.1498022212060306 1103 4 0.2317460800368707 1103 5 0.18510217903481418 1103 6 0.16470634915764037 1103 7 0.22395179140566057 1103 8 0.15276628290192917 1103 9 0.20107853513280816 1103 10-11 0.14118011647619966 1103 12-13 0.18330228584905228 1103 14-15 0.16717875547633554 1103 16-17 0.20660443834448472 1103 18-19 0.21712722734980616 1103 20-21 0.20693342598776354 1103 22-23 0.18969278546416746 1103 24-25 0.24066850683854568 1103 26-27 0.1641350555333574 1103 28-29 0.18202307788682504 1103 30-31 0.18370043982383777 1103 32-33 0.15680164386583328 1103 34-35 0.13606981013089126 1103 36-37 0.1278376868971094 1103 38-39 0.1769668472481527 1103 40-41 0.13502408533428678 1103 42-43 0.14385452484116001 1103 44-45 0.056367101351590065 1103 46-47 0.12167615718713165 1103 48-49 0.10576501661856241 1103 50-51 0.035027351444533394 1103 52-53 0.06515316223463685 1103 54-55 0.14480793957387306 1103 56-57 0.16424254355918322 1103 58-59 0.1944976832614813 1103 60-61 0.20484578392342456 1103 62-63 0.2106266430039696 1103 64-65 0.22459752945242428 1103 66-67 0.23342205853504083 1103 68-69 0.24091745068953685 1103 70-71 0.22379320580767015 1103 72-73 0.30128743946582404 1103 74-75 0.260114274956738 1103 76-77 0.170492367730656 1103 78-79 0.21094631319360246 1103 80-81 0.20698084596942934 1103 82-83 0.20199199226546583 1103 84-85 0.18177149185931185 1103 86-87 0.24840644285742997 1103 88-89 0.25625572803605223 1103 90-91 0.20500383948748535 1103 92-93 0.17310161734170393 1103 94-95 0.12605573624044197 1103 96-97 0.20776082321757627 1103 98-99 0.2065375481221814 1103 100-101 0.4290916844185446 1104 1 0.059374392259719855 1104 2 0.056907875462954394 1104 3 0.05576711802719103 1104 4 0.1878045139715141 1104 5 0.2397767053345916 1104 6 0.19549077950400573 1104 7 0.1853801413445666 1104 8 0.1230143431635966 1104 9 0.15599334257528596 1104 10-11 0.11904532659220024 1104 12-13 0.08292744279586373 1104 14-15 0.11656397530571638 1104 16-17 0.10539384741780111 1104 18-19 0.1323208742278723 1104 20-21 0.0867936754075842 1104 22-23 0.12752280061468468 1104 24-25 0.12962643726555712 1104 26-27 0.07669002301378214 1104 28-29 0.026193310312280005 1104 30-31 0.01351897639606392 1104 32-33 0.030904384555057618 1104 34-35 0.017484292008880686 1104 36-37 -0.004886533732921805 1104 38-39 0.08104482977351779 1104 40-41 0.04501596620657722 1104 42-43 0.004612677505889451 1104 44-45 -0.04500096955076316 1104 46-47 0.0388187225883172 1104 48-49 -0.024248950546081005 1104 50-51 -0.056836918347322296 1104 52-53 -0.05180413507233084 1104 54-55 0.02067251757396349 1104 56-57 0.005102755035807149 1104 58-59 0.03169945978012123 1104 60-61 0.045956853355669125 1104 62-63 -0.005950683832331549 1104 64-65 -0.01687680797921587 1104 66-67 0.0338984252836525 1104 68-69 0.08820664342202633 1104 70-71 0.10392317890982739 1104 72-73 0.11724199496548948 1104 74-75 0.12572945125850055 1104 76-77 0.07505392516386067 1104 78-79 0.05218674682944169 1104 80-81 0.10036104244462507 1104 82-83 0.10077371206803676 1104 84-85 0.1197388278896021 1104 86-87 0.1185328744637566 1104 88-89 0.08762776470298306 1104 90-91 0.13134517934137335 1104 92-93 0.09758247347184934 1104 94-95 0.013448495689566187 1104 96-97 0.08366999571360623 1104 98-99 0.10789993343913906 1104 100-101 0.16055879998232925 1105 1 0.137128530510644 1105 2 0.04618982925934745 1105 3 0.061617458469868325 1105 4 0.144261729099604 1105 5 0.18221510256066153 1105 6 0.11200123681045682 1105 7 0.15145440696024792 1105 8 0.10429603451833458 1105 9 0.1250972101252117 1105 10-11 0.03106080207673756 1105 12-13 0.03261406977686221 1105 14-15 0.13856711353682982 1105 16-17 0.1094081360834025 1105 18-19 0.08056194683688744 1105 20-21 0.130096858550786 1105 22-23 0.13170622421888822 1105 24-25 0.15862465511456492 1105 26-27 0.10476468908259307 1105 28-29 0.14917688029574094 1105 30-31 0.15746285824150874 1105 32-33 0.16435831495648756 1105 34-35 0.1057975879794597 1105 36-37 0.08842594029309936 1105 38-39 0.14983052331470503 1105 40-41 0.17504980787907698 1105 42-43 0.12186784696586983 1105 44-45 0.08811866138180591 1105 46-47 0.08512418909192121 1105 48-49 0.055406553866113484 1105 50-51 -0.022137019061581498 1105 52-53 -0.008328413177082439 1105 54-55 0.037113317450938155 1105 56-57 0.05196056984961217 1105 58-59 0.07870416378663947 1105 60-61 0.09630334034411447 1105 62-63 0.19543367468484263 1105 64-65 0.19225495144521432 1105 66-67 0.1104027125203828 1105 68-69 0.05549482265740835 1105 70-71 0.12678504261491597 1105 72-73 0.20708416128172757 1105 74-75 0.13292644874022486 1105 76-77 0.11590062876767604 1105 78-79 0.2035943215188638 1105 80-81 0.1698951876655599 1105 82-83 0.08711100753784606 1105 84-85 0.18639312623562532 1105 86-87 0.24719028740094373 1105 88-89 0.20112184338114503 1105 90-91 0.2102224571944511 1105 92-93 0.2358514205543223 1105 94-95 0.26411078674980004 1105 96-97 0.20340399231861284 1105 98-99 0.2731348583408888 1105 100-101 0.4334286490677215 1106 1 0.13107407021019313 1106 2 0.10429674394887911 1106 3 0.12212713903315375 1106 4 0.15946995941370545 1106 5 0.16044815050025818 1106 6 0.14989665475258107 1106 7 0.09515552325910903 1106 8 0.08475348634944879 1106 9 0.05608797852442393 1106 10-11 0.03973312702705556 1106 12-13 0.06393767151075735 1106 14-15 0.032216738985873405 1106 16-17 0.03876711126451937 1106 18-19 -0.0031494587739331337 1106 20-21 -0.004509413326076128 1106 22-23 0.025594909185485903 1106 24-25 0.07478558371565214 1106 26-27 -0.07198874553744616 1106 28-29 -0.0030774614407818035 1106 30-31 -5.396492588616297E-4 1106 32-33 -0.1002234361407659 1106 34-35 -0.06489606195892605 1106 36-37 -0.050299333145318315 1106 38-39 0.0906446177123712 1106 40-41 -0.025771315809898 1106 42-43 -0.04745247788636675 1106 44-45 -0.12341833456012097 1106 46-47 -0.1650630320956381 1106 48-49 -0.07960751814029265 1106 50-51 -0.16647955981573404 1106 52-53 -0.13252459774561487 1106 54-55 0.005904484723878056 1106 56-57 -0.04174291057984192 1106 58-59 0.013614066983485884 1106 60-61 0.07982820895445286 1106 62-63 0.08776035864651277 1106 64-65 0.05431238632640856 1106 66-67 0.09271968670272202 1106 68-69 0.08379335812595912 1106 70-71 0.049264114587035834 1106 72-73 0.15187961063215738 1106 74-75 0.14293412733379185 1106 76-77 0.03970425205902117 1106 78-79 0.10662428739141916 1106 80-81 0.07066306138634104 1106 82-83 0.08156900353795393 1106 84-85 0.11233412605201565 1106 86-87 0.11111378827324359 1106 88-89 0.11080768625064508 1106 90-91 0.1501290294894666 1106 92-93 0.17147020518872935 1106 94-95 0.15294807893737428 1106 96-97 0.11299700633921361 1106 98-99 0.11159638099113423 1106 100-101 0.22475540992990162 1107 1 0.18477614794158725 1107 2 0.058995409873105586 1107 3 0.12740111748213678 1107 4 0.18174506349290453 1107 5 0.1778520765774516 1107 6 0.19062855625381303 1107 7 0.14202611995404624 1107 8 0.1031474774051162 1107 9 0.12709885655374364 1107 10-11 0.09028887253401052 1107 12-13 0.11821693196503702 1107 14-15 0.08608549767540552 1107 16-17 0.15192112774228406 1107 18-19 0.04997344625711975 1107 20-21 0.010355526306625507 1107 22-23 0.0312729456001648 1107 24-25 0.05887576907397829 1107 26-27 -7.971018150811915E-4 1107 28-29 -0.0783032265820296 1107 30-31 -0.03499598456244968 1107 32-33 0.0058507035803927465 1107 34-35 0.003538793861402212 1107 36-37 -0.015793198852755097 1107 38-39 -0.03019604373591278 1107 40-41 -0.001853504701088582 1107 42-43 -0.009834477949574705 1107 44-45 -0.05559035459102546 1107 46-47 0.016004056889919127 1107 48-49 -0.03802180998512483 1107 50-51 -0.13497216829977532 1107 52-53 -0.09733936690938094 1107 54-55 0.01744638745014271 1107 56-57 0.001140934298135221 1107 58-59 0.016560749731432622 1107 60-61 -0.015602504831491615 1107 62-63 0.013182346263171496 1107 64-65 -0.0669358923381651 1107 66-67 0.0035364959871770907 1107 68-69 -0.028274867384830316 1107 70-71 0.01894487678515233 1107 72-73 0.020310872088515453 1107 74-75 -0.05761548580035125 1107 76-77 -0.020970397554485487 1107 78-79 -0.009680423246862802 1107 80-81 -0.06687661108187726 1107 82-83 -0.01531812467155902 1107 84-85 -0.07722177424919252 1107 86-87 0.04134560620789429 1107 88-89 -0.032880919753406346 1107 90-91 0.01574703644894271 1107 92-93 0.03211634240259542 1107 94-95 0.11386390042970262 1107 96-97 0.05124327602572265 1107 98-99 0.09743041568978938 1107 100-101 0.3395698876853537 1108 1 0.04115030042557777 1108 2 0.009599112149231814 1108 3 0.06149065937187714 1108 4 0.06389148235772524 1108 5 0.061097369497638 1108 6 0.018510269594401052 1108 7 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52-53 -0.29988627875944474 2316 54-55 -0.4009866631641543 2316 56-57 -0.36097369257944223 2316 58-59 -0.4067498961061702 2316 60-61 -0.40695989465006477 2316 62-63 -0.34778067851793537 2316 64-65 -0.3531607451833523 2316 66-67 -0.337577262699142 2316 68-69 -0.38524212130376156 2316 70-71 -0.35356885715014386 2316 72-73 -0.27247932834523425 2316 74-75 -0.3355646976688291 2316 76-77 -0.3856108083565708 2316 78-79 -0.33927627243359026 2316 80-81 -0.32544776703519673 2316 82-83 -0.34267187582906544 2316 84-85 -0.37427530436730194 2316 86-87 -0.3755299440871731 2316 88-89 -0.4376648024681238 2316 90-91 -0.47664181484135426 2316 92-93 -0.46204661754198995 2316 94-95 -0.4241032894296417 2316 96-97 -0.42679677974533803 2316 98-99 -0.44807578668878634 2316 100-101 -0.7111957904020265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 273976.0 3 68322.0 4 65140.0 5 74086.0 6 103855.0 7 183889.0 8 377565.0 9 744847.0 10 971556.0 11 756234.0 12 504294.0 13 375071.0 14 305441.0 15 250170.0 16 214643.0 17 191147.0 18 181280.0 19 177431.0 20 183864.0 21 196020.0 22 211422.0 23 234883.0 24 267810.0 25 288584.0 26 272753.0 27 302580.0 28 341780.0 29 389266.0 30 452595.0 31 536777.0 32 663100.0 33 845168.0 34 1140093.0 35 1680008.0 36 2807581.0 37 6107243.0 38 1.1644749E7 39 5503892.0 40 17491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.998702335788014 16.50899141323813 8.48887011343078 46.003436137543076 2 11.601616831977331 16.16477534602974 60.95509695805939 11.278510863933535 3 17.134003080572192 20.65052925167363 41.60216800854659 20.613299659207584 4 18.4938060408032 27.478836366404696 37.584268545667186 16.443089047124918 5 19.18162763301679 30.30215065483303 36.661190620501706 13.85503109164847 6 14.776577190978092 33.070510711848705 38.47076062274981 13.682151474423392 7 12.599375605782868 17.659440495363548 55.5829648834879 14.158219015365692 8 12.708712103597684 20.584750807604294 48.96011086666148 17.74642622213654 9 14.077236072302442 20.445316110250882 47.81167121231208 17.665776605134596 10-11 17.84167562345391 30.252353203306185 36.7234978417737 15.182473331466204 12-13 16.539029669461314 24.01802991653789 42.92010946435765 16.522830949643144 14-15 17.017024673788406 25.48145362529837 41.49781154164594 16.003710159267285 16-17 17.973691293821815 25.83474208176352 39.750966975458304 16.440599648956365 18-19 18.022395123773123 26.847304138015566 38.66853482977079 16.46176590844052 20-21 18.357433561539832 27.112047946347236 37.61777011971132 16.912748372401616 22-23 18.9146364884085 28.0089346478428 36.440066865987895 16.636361997760808 24-25 18.342524798600433 28.57940216365682 35.89275331686766 17.18531972087509 26-27 18.404447833223564 29.161546232426833 34.85202541400364 17.58198052034596 28-29 18.635895963528913 29.720901776151464 33.74049167793397 17.90271058238566 30-31 18.74876589202726 29.675948162152704 33.55065048819088 18.024635457629156 32-33 18.848417267410852 30.09946018453894 32.85632038012739 18.195802167922817 34-35 19.191359473889964 30.544200676691684 32.219291076885185 18.045148772533175 36-37 18.91072539455777 30.0836075020159 32.737503310071546 18.268163793354788 38-39 19.16302430321392 29.566902760861208 32.87575600774342 18.394316928181453 40-41 19.277769720961977 29.367163831089126 33.14200984781926 18.213056600129633 42-43 19.59189153465467 29.22390952146805 33.29778573258232 17.88641321129496 44-45 19.74023567069517 29.17226495448236 33.11010893973981 17.977390435082665 46-47 18.918755729145158 29.466345603592302 32.99135767869506 18.623540988567473 48-49 19.20216637524976 29.578045657352252 32.28301532522475 18.936772642173235 50-51 18.914535663821532 29.71551136469367 32.71998875661313 18.649964214871677 52-53 19.145637995256937 30.280543013032464 31.996628046440968 18.577190945269635 54-55 18.96545714468551 30.45371394449064 31.30893363151771 19.271895279306136 56-57 19.2117447805369 30.53234549222546 31.487264480900567 18.768645246337073 58-59 19.11499660988109 30.70464408914938 31.190184445861323 18.990174855108204 60-61 19.182612494933473 31.309683075440763 30.37559169652419 19.132112733101575 62-63 19.31548592193474 31.148652172692003 30.75643898970123 18.77942291567203 64-65 19.76110238628244 30.708214976300503 30.683985518456474 18.84669711896058 66-67 19.52697484377182 31.376586761417542 30.22153586185143 18.874902532959208 68-69 19.820747703818316 30.883103932841944 30.589973128997055 18.706175234342687 70-71 19.71920320904168 30.919517078464757 30.296205676532857 19.065074035960706 72-73 19.325995131320052 31.223747683050785 30.387699908074854 19.06255727755431 74-75 19.156394830877552 30.603433503959838 31.02757872188934 19.212592943273275 76-77 19.364868273905714 30.98509134473938 30.532045578192097 19.117994803162812 78-79 19.46811890558321 31.11798500207465 30.241451453685723 19.172444638656415 80-81 19.416728481146343 30.786599343990034 30.510578342872485 19.286093831991135 82-83 19.67931961910738 31.041946803687026 30.091392026098834 19.187341551106762 84-85 19.45416331822163 30.947961724835626 30.263097517400013 19.33477743954273 86-87 19.79301706518848 31.109354848683974 29.955301023637855 19.142327062489695 88-89 19.515607432141007 31.169233279874724 30.10294320465532 19.212216083328947 90-91 19.695818652766583 31.099780739055433 30.169701393253707 19.034699214924274 92-93 19.889440017317746 30.823383664327846 30.097249450244146 19.189926868110266 94-95 19.65048319199675 30.82891277156048 30.211292150002823 19.309311886439946 96-97 19.420513192590544 31.141932246026066 29.976011647454182 19.461542913929204 98-99 19.48934801969311 31.026839148186852 29.973459755455067 19.51035307666497 100-101 19.870709386227155 31.45725007434907 29.31631419077026 19.355726348653516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11479.0 1 19900.5 2 26539.5 3 29435.0 4 40007.0 5 52448.5 6 65339.5 7 79203.5 8 93934.0 9 108294.0 10 122238.0 11 134751.0 12 147032.0 13 158452.5 14 168856.0 15 179677.0 16 191002.5 17 202688.5 18 219354.5 19 243420.0 20 278466.0 21 322705.0 22 369832.0 23 433764.0 24 509991.0 25 585582.0 26 678270.0 27 786731.0 28 915396.5 29 1037944.5 30 1182375.5 31 1312206.0 32 1422307.0 33 1495706.5 34 1458380.0 35 1461542.0 36 1521736.0 37 1569195.5 38 1597442.5 39 1507028.5 40 1408739.0 41 1356112.0 42 1270345.0 43 1210711.5 44 1133525.0 45 1068645.0 46 1042077.5 47 1008103.0 48 965761.0 49 913964.0 50 829146.5 51 727335.5 52 658876.5 53 607259.5 54 542329.0 55 464320.5 56 400169.0 57 334505.5 58 268946.0 59 219317.5 60 168143.0 61 125115.5 62 95945.0 63 71366.0 64 49239.5 65 33721.0 66 23052.5 67 17436.5 68 13551.0 69 10035.0 70 10204.5 71 14910.0 72 14887.5 73 21727.5 74 23545.5 75 12459.0 76 8323.0 77 9337.0 78 8635.5 79 4168.0 80 2335.5 81 1281.5 82 812.5 83 479.0 84 235.5 85 153.0 86 128.5 87 84.5 88 48.0 89 31.0 90 26.0 91 23.5 92 19.5 93 13.0 94 7.5 95 6.0 96 4.0 97 2.5 98 3.0 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6611411654501513 2 0.31535881553044126 3 0.31006645867102806 4 0.33109054676310984 5 0.3128855408049484 6 0.10925258840603985 7 0.09974539052506745 8 0.09384912362629887 9 0.12106516900986268 10-11 0.09954617538760376 12-13 0.12157886842093261 14-15 0.1147266194474168 16-17 0.10678933708368986 18-19 0.132411661367544 20-21 0.12459591276692385 22-23 0.11147653097835482 24-25 0.1388629742153467 26-27 0.1160171326020559 28-29 0.13667661940481732 30-31 0.11865328762861967 32-33 0.12442050321192437 34-35 0.14282221845676377 36-37 0.1277282262490576 38-39 0.1305635964130851 40-41 0.1185881355081913 42-43 0.10931774052646821 44-45 0.42071605889009955 46-47 0.08588678275471484 48-49 0.07990907570541078 50-51 0.08144015053547776 52-53 0.2803307803224358 54-55 0.32264457668988433 56-57 0.2544791706916895 58-59 0.2702672835670365 60-61 0.06818419987908769 62-63 0.06546785762738129 64-65 0.08950397836388291 66-67 0.09200982914958993 68-69 0.08724244802978233 70-71 0.10429476262651853 72-73 0.14230726612030098 74-75 0.11931859101222489 76-77 0.10132407652006287 78-79 0.09328029549794337 80-81 0.09179557890741197 82-83 0.09639256217379148 84-85 0.09204365813519697 86-87 0.11545331617527184 88-89 0.11413774451277565 90-91 0.15211641902095108 92-93 0.14649955448478882 94-95 0.12331166423924901 96-97 0.10119502520459896 98-99 0.13606268596231913 100-101 0.1186069293890841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.9906606E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.79274477741075 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9063991850913 20.87326269893232 2 8.234495494524745 4.412494723110803 3 3.5060639126471433 2.8181122675453514 4 1.9778867357087175 2.119720580338789 5 1.3312933076475302 1.783450090783762 6 0.9050280777017561 1.4548911781352287 7 0.708736349584233 1.3292294490218883 8 0.5385327934828565 1.1543017352041778 9 0.45915657291163947 1.107185838380293 >10 3.599692222715504 19.328071177372998 >50 0.45194461075121095 8.488359832490417 >100 0.3344074337329497 17.488703171564705 >500 0.02942511636002465 5.348624365174837 >1k 0.015485936671915066 7.8110787701037365 >5k 9.650438667031557E-4 1.7068513899234208 >10k+ 4.8720660189097495E-4 2.77566273191716 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 109180 0.27358878878349113 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77636 0.19454423159914927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.3317997025354649 2.505850785707008E-6 0.0 0.0 0.0 2 0.3336014092503883 2.505850785707008E-6 0.0 0.0 0.0 3 0.3338319475226733 2.505850785707008E-6 0.0 0.0 0.0 4 0.3468799125638497 5.011701571414016E-6 0.0 0.0 0.0 5 0.3474938460063479 5.011701571414016E-6 0.0 0.0 0.0 6 0.34822304858498865 1.0023403142828032E-5 0.0 0.0 0.0 7 0.34929304687048557 1.0023403142828032E-5 0.0 2.505850785707008E-6 0.0 8 0.3558633876306093 1.0023403142828032E-5 0.0 7.517552357121024E-6 0.0 9 0.356362051936965 1.0023403142828032E-5 0.0 7.517552357121024E-6 0.0 10-11 0.3599742358445617 1.1276328535681536E-5 0.0 1.0023403142828032E-5 0.0 12-13 0.36328947643405207 1.3782179321388543E-5 0.0 1.0023403142828032E-5 0.0 14-15 0.3663754316766502 1.5035104714242047E-5 0.0 1.1276328535681536E-5 0.0 16-17 0.3708358460752087 1.5035104714242047E-5 0.0 1.252925392853504E-5 0.0 18-19 0.37582750084033706 1.7540955499949055E-5 0.0 1.252925392853504E-5 0.0 20-21 0.38341646994485074 1.7540955499949055E-5 0.0 1.252925392853504E-5 0.0 22-23 0.3903990231592233 1.7540955499949055E-5 0.0 1.252925392853504E-5 0.0 24-25 0.3952716500120306 1.7540955499949055E-5 0.0 1.3782179321388543E-5 0.0 26-27 0.3998385630689816 1.7540955499949055E-5 0.0 1.6288030107095552E-5 0.0 28-29 0.4040985094046835 1.7540955499949055E-5 0.0 1.8793880892802557E-5 2.505850785707008E-6 30-31 0.4083321443071355 2.129973167850957E-5 0.0 2.0046806285656063E-5 2.505850785707008E-6 32-33 0.4127900528549083 2.2552657071363072E-5 0.0 2.0046806285656063E-5 2.505850785707008E-6 34-35 0.41828788947874945 2.3805582464216575E-5 0.0 2.129973167850957E-5 2.505850785707008E-6 36-37 0.42278839748987923 2.505850785707008E-5 0.0 2.3805582464216575E-5 2.505850785707008E-6 38-39 0.42749062648925845 2.6311433249923587E-5 0.0 2.505850785707008E-5 2.505850785707008E-6 40-41 0.4325737448080651 2.8817284035630592E-5 0.0 2.505850785707008E-5 2.505850785707008E-6 42-43 0.43773078572505014 3.0070209428484095E-5 0.0 2.505850785707008E-5 2.505850785707008E-6 44-45 0.44313340001903445 3.2576060214191104E-5 0.0 2.505850785707008E-5 2.505850785707008E-6 46-47 0.4502437516234781 3.38289856070446E-5 0.0 2.756435864277709E-5 2.505850785707008E-6 48-49 0.45622396452356784 3.6334836392751615E-5 0.0 2.8817284035630592E-5 2.505850785707008E-6 50-51 0.4620325266448367 3.758776178560512E-5 0.0 3.13231348213376E-5 2.505850785707008E-6 52-53 0.46824202489181865 3.758776178560512E-5 0.0 3.2576060214191104E-5 2.505850785707008E-6 54-55 0.4750992855669059 3.758776178560512E-5 0.0 3.2576060214191104E-5 2.505850785707008E-6 56-57 0.48262936717795546 3.758776178560512E-5 0.0 3.5081910999898116E-5 2.505850785707008E-6 58-59 0.4901845072968621 3.758776178560512E-5 0.0 3.758776178560512E-5 2.505850785707008E-6 60-61 0.49761184902569766 3.758776178560512E-5 0.0 4.009361257131213E-5 2.505850785707008E-6 62-63 0.5056581358986028 4.009361257131213E-5 0.0 4.259946335701914E-5 2.505850785707008E-6 64-65 0.5139374668945789 4.009361257131213E-5 0.0 4.385238874987264E-5 2.505850785707008E-6 66-67 0.5233193722362659 4.009361257131213E-5 0.0 4.5105314142726144E-5 2.505850785707008E-6 68-69 0.533521943710272 4.009361257131213E-5 0.0 4.5105314142726144E-5 2.505850785707008E-6 70-71 0.5440076763230629 4.5105314142726144E-5 0.0 4.5105314142726144E-5 2.505850785707008E-6 72-73 0.5554143090996012 4.761116492843315E-5 0.0 4.5105314142726144E-5 2.505850785707008E-6 74-75 0.5677894532048153 5.136994110699366E-5 0.0 4.5105314142726144E-5 2.505850785707008E-6 76-77 0.5812508836256334 5.262286649984717E-5 0.0 5.387579189270067E-5 2.505850785707008E-6 78-79 0.5962133186670898 5.262286649984717E-5 0.0 5.512871728555418E-5 2.505850785707008E-6 80-81 0.6123610211301859 5.262286649984717E-5 0.0 6.01404188569682E-5 2.505850785707008E-6 82-83 0.6305823151184543 5.512871728555418E-5 0.0 6.515212042838221E-5 2.505850785707008E-6 84-85 0.6508233248400027 5.512871728555418E-5 0.0 6.515212042838221E-5 2.505850785707008E-6 86-87 0.6740914023106852 5.7634568071261185E-5 0.0 6.765797121408922E-5 2.505850785707008E-6 88-89 0.7006872496247865 5.8887493464114684E-5 0.0 7.016382199979622E-5 2.505850785707008E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGGC 12210 0.0 68.92264 9 CGCGTGT 21295 0.0 65.498215 1 AAGACGG 14300 0.0 59.280735 8 GTTTTTT 726955 0.0 50.328262 1 GCGTGTT 30980 0.0 45.15678 2 GAAGACG 20730 0.0 40.983936 7 CGGCATA 10665 0.0 39.16601 12-13 GACGGCA 11775 0.0 35.83502 10-11 TACGAGA 11660 0.0 35.457092 16-17 AGATCGG 37765 0.0 34.798607 1 ACGGCAT 12215 0.0 34.097584 10-11 ATACGAG 12545 0.0 33.504055 16-17 GATCGGA 44230 0.0 30.070793 2 CGAGATT 13840 0.0 29.703148 18-19 ATCGGAA 44885 0.0 29.675121 3 CATACGA 14460 0.0 29.132582 14-15 TCGGAAG 46135 0.0 28.73678 4 ACGAGAT 14865 0.0 28.69226 18-19 CGCCGTA 33200 0.0 28.503572 46-47 CGGAAGA 46820 0.0 28.323011 5 >>END_MODULE