FastQCFastQC Report
Fri 27 May 2016
SRR1233084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39906606
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1137240.2849753747537438TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC735460.1842953018856076TruSeq Adapter, Index 4 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT681920.1708789767789323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT209150.067.923441
GTTTTTT6762950.049.731761
GCGTGTT299450.047.3840832
AGATCGG362250.037.0669631
CGTGTTT491900.029.6935753
GATCGGA454850.029.5984462
ATCGGAA458650.029.4036753
TCGGAAG477900.028.167784
CTAACGA115600.028.04492894-95
CGGAAGA482000.027.7919185
CCCGAAA127950.027.015131
CTCGTAT389250.026.74535242-43
TCGTCTA127050.025.79176144-45
CTCCGTT56150.025.283511
TCGTACC29400.024.542972
ACTCGTC134450.024.5142942-43
ATCTCGT418300.024.32486540-41
CCGAAAC142550.023.943772
CGAGCTA139300.023.77486612-13
CGTCTGA471900.023.60957716-17
Produced by FastQC (version 0.11.5)