FastQCFastQC Report
Fri 27 May 2016
SRR1233083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64137862
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2045200.3188756120370835No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2029610.31644491049608103Illumina Single End PCR Primer 1 (100% over 50bp)
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1919920.2993426877871296No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1435110.22375395051366073No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1354860.2112418402721313No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1316540.20526721018545957No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1153500.17984696777076853No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1148630.1790876658782296No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA885250.13802299802260326No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT806920.12581024294199267No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT794430.12386287525455714No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT768410.11980598916752168No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT735510.1146764137538604No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA723530.11280856228104391No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC702580.10954216091580975No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT652860.10179010956118244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT9447400.050.751161
CGCGTGT353150.050.6731951
GCGTGTT436900.041.003122
AGAGCGT652050.039.959919
CGCCGTA398850.039.92796746-47
CCGTATC409400.039.2671248-49
AAGAGCG683650.039.1055768
GTCGCCG409950.038.08128744-45
AGATCGG721200.036.9056661
TCCGATC70300.036.016221
ATCGGAA759200.035.2601663
CGGAAGA767650.034.6670885
TCGGTGG462750.034.66340338-39
GATCGGA771500.034.629842
ATCAATA575850.034.340734
TGGTCGC427950.034.20618442-43
CCGATCT75700.034.0939062
GATCTCG512150.033.486334-35
CCTTTAG432400.033.4272655
TCGTACC145800.032.9584272
Produced by FastQC (version 0.11.5)