##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233083_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64137862 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82067946075284 33.0 31.0 34.0 27.0 34.0 2 31.219224083272373 33.0 31.0 34.0 27.0 34.0 3 31.11736677471413 34.0 31.0 34.0 27.0 34.0 4 34.39469440063343 37.0 35.0 37.0 30.0 37.0 5 34.34888590455354 37.0 35.0 37.0 30.0 37.0 6 34.39931653786651 37.0 35.0 37.0 30.0 37.0 7 34.497876293413086 37.0 35.0 37.0 31.0 37.0 8 34.48431787451849 37.0 35.0 37.0 30.0 37.0 9 36.00667710127288 39.0 37.0 39.0 30.0 39.0 10-11 35.856492737472294 39.0 37.0 39.0 30.0 39.0 12-13 35.75971686583503 39.0 37.0 39.0 30.0 39.0 14-15 36.91168233827314 40.0 37.0 41.0 30.0 41.0 16-17 36.66494044500579 40.0 37.0 41.0 29.5 41.0 18-19 36.18878402120732 40.0 36.0 41.0 27.0 41.0 20-21 35.835090324650984 39.5 35.0 41.0 27.0 41.0 22-23 35.40024514693053 39.0 35.0 41.0 25.0 41.0 24-25 34.93618890819903 39.0 35.0 41.0 20.0 41.0 26-27 34.45080147822826 39.0 34.5 41.0 16.0 41.0 28-29 34.25506349120275 39.0 34.5 41.0 10.5 41.0 30-31 34.03434217997476 40.0 34.0 41.0 9.0 41.0 32-33 33.87756062090127 40.0 34.0 41.0 8.0 41.0 34-35 33.72643971980232 39.0 34.0 41.0 6.0 41.0 36-37 33.58470584816189 39.0 34.0 41.0 2.0 41.0 38-39 33.4489252775529 39.0 34.0 41.0 2.0 41.0 40-41 33.32065358680026 39.0 33.5 41.0 2.0 41.0 42-43 33.15742359014088 39.0 33.0 41.0 2.0 41.0 44-45 32.874892064534365 39.0 33.0 41.0 2.0 41.0 46-47 32.80651162803026 39.0 33.0 41.0 2.0 41.0 48-49 32.69104504138289 39.0 33.0 41.0 2.0 41.0 50-51 32.17179459458752 38.5 32.0 40.5 2.0 40.5 52-53 32.22061184390587 38.5 32.5 40.0 2.0 41.0 54-55 32.543513151405016 39.0 33.0 41.0 2.0 41.0 56-57 32.4019070669989 39.0 32.5 41.0 2.0 41.0 58-59 32.27703044576073 39.0 32.0 41.0 2.0 41.0 60-61 32.19184276052108 38.5 32.0 41.0 2.0 41.0 62-63 31.98149541529775 38.0 31.5 41.0 2.0 41.0 64-65 31.678650412450605 38.0 31.0 40.0 2.0 41.0 66-67 31.351322678950538 37.5 31.0 40.0 2.0 41.0 68-69 31.016217612617023 37.0 31.0 40.0 2.0 41.0 70-71 30.674139597918 36.5 30.0 39.0 2.0 41.0 72-73 30.32729777459685 36.0 30.0 39.0 2.0 41.0 74-75 29.912147905709734 35.5 29.0 39.0 2.0 40.5 76-77 29.537721595708945 35.0 29.0 37.5 2.0 40.0 78-79 29.149868879321232 35.0 29.0 37.0 2.0 39.0 80-81 28.76250033092778 35.0 28.5 37.0 2.0 39.0 82-83 28.397437156542573 35.0 28.0 36.0 2.0 39.0 84-85 28.03104217911099 35.0 27.0 36.0 2.0 37.0 86-87 27.686630628566945 34.0 26.0 35.5 2.0 37.0 88-89 27.383878916949243 34.0 26.0 35.0 2.0 36.5 90-91 27.11922021192412 34.0 26.0 35.0 2.0 36.0 92-93 26.818566410897823 34.0 25.0 35.0 2.0 36.0 94-95 26.473982325759472 34.0 24.5 35.0 2.0 36.0 96-97 26.1341054212253 34.0 23.5 35.0 2.0 35.0 98-99 25.788327228618876 34.0 19.0 35.0 2.0 35.0 100-101 24.116027261713214 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5822390287710562 1101 2 0.6006630427983737 1101 3 0.7291434264989576 1101 4 0.7656588064951251 1101 5 0.7989854683979942 1101 6 0.7961356502210464 1101 7 0.7700768084180041 1101 8 0.7260056077609818 1101 9 0.8027551639625585 1101 10-11 0.836386902263051 1101 12-13 0.8827214723498855 1101 14-15 0.8784639058557246 1101 16-17 0.9968905015833442 1101 18-19 1.0381046758096417 1101 20-21 0.9914882720958786 1101 22-23 0.900638974712642 1101 24-25 0.9966066907788971 1101 26-27 1.010185990038444 1101 28-29 1.0102966456099765 1101 30-31 1.015379878198921 1101 32-33 1.0081069860527307 1101 34-35 0.9219608194874311 1101 36-37 0.9575862910109905 1101 38-39 0.9357588150023801 1101 40-41 0.8755527847959854 1101 42-43 0.929638587290512 1101 44-45 0.7933855441953526 1101 46-47 0.8227368916989022 1101 48-49 0.8117119024443156 1101 50-51 0.8223832345406521 1101 52-53 0.7597903765812433 1101 54-55 0.7697695537380227 1101 56-57 0.8648800152004199 1101 58-59 0.9389158485240827 1101 60-61 0.9335693525396849 1101 62-63 0.9228487530930352 1101 64-65 0.9242149706272222 1101 66-67 0.9635914024575385 1101 68-69 0.8821348289400035 1101 70-71 0.8020461801122067 1101 72-73 0.8408853811243766 1101 74-75 0.809155484296717 1101 76-77 0.8257722233770508 1101 78-79 0.8594149283855081 1101 80-81 0.8114983840285674 1101 82-83 0.7942216051304776 1101 84-85 0.7234912787004788 1101 86-87 0.7531877572222889 1101 88-89 0.820821283689579 1101 90-91 0.8108319560725441 1101 92-93 0.8381803002889754 1101 94-95 0.8939746767065238 1101 96-97 0.9411039971897281 1101 98-99 0.8674029263571157 1101 100-101 0.9833676600987395 1102 1 0.4897108126528593 1102 2 0.4750951851277456 1102 3 0.6033395935793351 1102 4 0.5861628008476956 1102 5 0.6029811345676634 1102 6 0.5679686873766343 1102 7 0.5809589194985492 1102 8 0.6155651403904372 1102 9 0.7393156737563231 1102 10-11 0.769772896285275 1102 12-13 0.7645291107987546 1102 14-15 0.7636181635562238 1102 16-17 0.8421125131940315 1102 18-19 0.8906980544698158 1102 20-21 0.8403645496325822 1102 22-23 0.8708464732659209 1102 24-25 0.8704863094161439 1102 26-27 0.9085950046634892 1102 28-29 0.83988503647317 1102 30-31 0.9044238512379152 1102 32-33 0.8912846397241836 1102 34-35 0.9294149503641336 1102 36-37 0.8573101693376586 1102 38-39 0.7618808903983378 1102 40-41 0.8421544535800933 1102 42-43 0.8040154059410796 1102 44-45 0.815539659055581 1102 46-47 0.8665072924564754 1102 48-49 0.8172618397879816 1102 50-51 0.7611197053366396 1102 52-53 0.7384669036946363 1102 54-55 0.8738483121033553 1102 56-57 0.9342861293716922 1102 58-59 0.9607132505157665 1102 60-61 0.958564589234129 1102 62-63 0.9287197702555545 1102 64-65 0.952220577836048 1102 66-67 0.9435898085537602 1102 68-69 0.9662758899370161 1102 70-71 0.8937414850955605 1102 72-73 0.7989238963114502 1102 74-75 0.8587743459146466 1102 76-77 0.8619534342198172 1102 78-79 0.88678413780789 1102 80-81 0.8552529728430862 1102 82-83 0.8860160561192245 1102 84-85 0.8504228796115427 1102 86-87 0.8184615376274458 1102 88-89 0.8170802502093082 1102 90-91 0.8190356141865927 1102 92-93 0.8306460193639857 1102 94-95 0.8020610218132056 1102 96-97 0.8236205713782532 1102 98-99 0.8581717945800484 1102 100-101 0.9584511958877862 1103 1 0.354676798740055 1103 2 0.402906694877867 1103 3 0.38465609702934955 1103 4 0.5313054473177488 1103 5 0.3612983569102468 1103 6 0.49221555585054944 1103 7 0.5449361523463381 1103 8 0.5218342129893827 1103 9 0.5772948030302274 1103 10-11 0.5909149304397516 1103 12-13 0.6372705335704794 1103 14-15 0.7085638562737273 1103 16-17 0.6742388537391975 1103 18-19 0.5784350847761388 1103 20-21 0.6811430182671856 1103 22-23 0.6605478321963858 1103 24-25 0.7466024659563786 1103 26-27 0.776864396770236 1103 28-29 0.722115522780598 1103 30-31 0.6051086757095874 1103 32-33 0.7127512996768957 1103 34-35 0.7797790426879416 1103 36-37 0.7573678827468058 1103 38-39 0.6302387523440771 1103 40-41 0.7005645271058967 1103 42-43 0.7302884634607096 1103 44-45 0.6880961821633136 1103 46-47 0.6619022409719904 1103 48-49 0.6474208050496983 1103 50-51 0.6581184459867799 1103 52-53 0.6120007754908485 1103 54-55 0.7036578820879456 1103 56-57 0.7239340916785082 1103 58-59 0.7384871157154151 1103 60-61 0.7591718765247535 1103 62-63 0.8129813430191959 1103 64-65 0.7641491126378206 1103 66-67 0.8046697292648304 1103 68-69 0.7915006736247108 1103 70-71 0.8200814515937225 1103 72-73 0.7951389919443166 1103 74-75 0.7725599350023309 1103 76-77 0.736782045973591 1103 78-79 0.7153315224231953 1103 80-81 0.7533257744331934 1103 82-83 0.7841191576434277 1103 84-85 0.735951058677415 1103 86-87 0.7162773021532232 1103 88-89 0.7083282258451504 1103 90-91 0.7127063968888443 1103 92-93 0.6784487107880643 1103 94-95 0.7555234180471935 1103 96-97 0.7870521522519951 1103 98-99 0.7477880679450735 1103 100-101 0.9019893922949649 1104 1 0.2804255392831827 1104 2 0.24665958944500943 1104 3 0.29108471808438097 1104 4 0.4882663313470772 1104 5 0.4555460228157173 1104 6 0.48111535646177117 1104 7 0.5016059198805607 1104 8 0.5194596609406403 1104 9 0.5624848127930164 1104 10-11 0.502379104272201 1104 12-13 0.5332948123854067 1104 14-15 0.6135283028685805 1104 16-17 0.6297091899048297 1104 18-19 0.5846244031307108 1104 20-21 0.5886279632251856 1104 22-23 0.5864033044781962 1104 24-25 0.5384882682915304 1104 26-27 0.5907413310065479 1104 28-29 0.5540050175133899 1104 30-31 0.574444994862084 1104 32-33 0.5337344405416786 1104 34-35 0.5371028804859108 1104 36-37 0.41974051751125074 1104 38-39 0.510255946902312 1104 40-41 0.4572004084054342 1104 42-43 0.48823876574851965 1104 44-45 0.49321624461386193 1104 46-47 0.495663115779557 1104 48-49 0.5037661954415782 1104 50-51 0.4691505621773615 1104 52-53 0.40781963390289633 1104 54-55 0.5126776778003688 1104 56-57 0.46755056148677454 1104 58-59 0.507388599306104 1104 60-61 0.519730269743377 1104 62-63 0.5192005519928031 1104 64-65 0.41715484415430026 1104 66-67 0.5312061326721249 1104 68-69 0.5559735401756676 1104 70-71 0.46467325119540703 1104 72-73 0.5645494811766447 1104 74-75 0.5460108338657719 1104 76-77 0.611082624364073 1104 78-79 0.5705942785574116 1104 80-81 0.5028571676663809 1104 82-83 0.5130233814679386 1104 84-85 0.5132267987175538 1104 86-87 0.45245104666762614 1104 88-89 0.4571410097033919 1104 90-91 0.4706126798128878 1104 92-93 0.4735041173852288 1104 94-95 0.4109190288078004 1104 96-97 0.45296677260757434 1104 98-99 0.4499178765657419 1104 100-101 0.2510376525337321 1105 1 0.1472774298194821 1105 2 0.19521050944963392 1105 3 0.2809396264278057 1105 4 0.33761776239252583 1105 5 0.3602751054456732 1105 6 0.38680579551695615 1105 7 0.322500527024161 1105 8 0.3329727035568766 1105 9 0.37986250637074903 1105 10-11 0.3931913872194812 1105 12-13 0.2968028619743066 1105 14-15 0.421724572361974 1105 16-17 0.3622334232785249 1105 18-19 0.38128173611935523 1105 20-21 0.2839182592463274 1105 22-23 0.33855003554215557 1105 24-25 0.28377814039132687 1105 26-27 0.269706940626115 1105 28-29 0.3250540263735573 1105 30-31 0.2516457121040716 1105 32-33 0.21223354117939408 1105 34-35 0.08716318590975192 1105 36-37 0.14909177002851237 1105 38-39 0.10811048997687323 1105 40-41 0.22799442176724938 1105 42-43 0.29458304111557254 1105 44-45 0.186279240770034 1105 46-47 0.23184128682633087 1105 48-49 0.1706774352833591 1105 50-51 0.1817384235178423 1105 52-53 0.1415811327061718 1105 54-55 0.28522901690775626 1105 56-57 0.28235451584537685 1105 58-59 0.1690839285964003 1105 60-61 0.2402369985085926 1105 62-63 0.2803637885975476 1105 64-65 0.23222327921187613 1105 66-67 0.32553240083537816 1105 68-69 0.2685236047646278 1105 70-71 0.24610891802169732 1105 72-73 0.29336242639735133 1105 74-75 0.2936860495032292 1105 76-77 0.29251974927084845 1105 78-79 0.2365477339624924 1105 80-81 0.29944616747787123 1105 82-83 0.18660530630122096 1105 84-85 0.20269083163147883 1105 86-87 0.2765752300673654 1105 88-89 0.25749909375593205 1105 90-91 0.2120427527769415 1105 92-93 0.16309178004304314 1105 94-95 0.1415112097643565 1105 96-97 0.07488815145130445 1105 98-99 0.012314385856150523 1105 100-101 0.023533949142258592 1106 1 -0.007928630783528234 1106 2 0.04113214059802672 1106 3 0.05072396491109643 1106 4 0.060312926427144475 1106 5 0.06351919163665798 1106 6 0.08986550880093347 1106 7 0.11397245023088232 1106 8 0.15726596728081432 1106 9 0.16541618556995985 1106 10-11 -0.006621799571462361 1106 12-13 0.05154366870441862 1106 14-15 0.03420618457756319 1106 16-17 0.027843448926937242 1106 18-19 -0.05274203015594736 1106 20-21 0.024286557799840125 1106 22-23 0.026623938093322863 1106 24-25 -0.1434917657317456 1106 26-27 -0.10927258792839467 1106 28-29 -0.026238220305039306 1106 30-31 0.054866497630989386 1106 32-33 -0.011917839872616298 1106 34-35 -0.12731769288804884 1106 36-37 -0.13630873683257505 1106 38-39 -0.18721039420166363 1106 40-41 -0.14927311420746037 1106 42-43 -0.0639937478214776 1106 44-45 -0.048935998032270334 1106 46-47 -0.16618216948763376 1106 48-49 -0.11567730911482244 1106 50-51 -0.09308159152654838 1106 52-53 -0.12551781865322198 1106 54-55 -0.006239391948106743 1106 56-57 0.057248181873312376 1106 58-59 -0.07011085997832112 1106 60-61 -0.010857236140680016 1106 62-63 0.04991552941354627 1106 64-65 0.05896993200640921 1106 66-67 0.06604657930797941 1106 68-69 0.03285719944145882 1106 70-71 -0.06038788236595849 1106 72-73 0.011477664868319692 1106 74-75 0.05873144073929737 1106 76-77 -0.015144184571880714 1106 78-79 0.006213710155371643 1106 80-81 0.03450275574958539 1106 82-83 0.06642906405746274 1106 84-85 0.09656541176968858 1106 86-87 -0.01895767721065056 1106 88-89 0.015091138380277869 1106 90-91 0.05125941301903225 1106 92-93 -0.01579202555332415 1106 94-95 -0.06581376545189244 1106 96-97 -0.09534261466637162 1106 98-99 -0.2643560060634371 1106 100-101 -0.31382163860833145 1107 1 4.331514699771333E-4 1107 2 0.04795725459717559 1107 3 0.0683038188160836 1107 4 0.12296998980638563 1107 5 -0.047073718351796856 1107 6 0.12709682665692412 1107 7 0.06151115507520899 1107 8 0.1361830228629941 1107 9 0.053449957796729564 1107 10-11 0.12559629138409178 1107 12-13 0.11139774084202969 1107 14-15 0.04347704391027918 1107 16-17 0.04543942881367258 1107 18-19 0.06918989115165175 1107 20-21 0.09059591212636064 1107 22-23 -0.01736789336958111 1107 24-25 -0.017113893053981144 1107 26-27 -0.05526774818498126 1107 28-29 -0.060592681943823834 1107 30-31 -0.02612464732604991 1107 32-33 -0.014989737971525585 1107 34-35 -0.06030791818828618 1107 36-37 -0.057911501498828954 1107 38-39 -0.10728310840043065 1107 40-41 -0.08077416766759171 1107 42-43 -0.043664602124295016 1107 44-45 -0.03753743128314824 1107 46-47 -0.06766346643190246 1107 48-49 -0.07875345068180195 1107 50-51 -0.18868380607388247 1107 52-53 -0.1575142300913015 1107 54-55 -0.02107894993704207 1107 56-57 0.016104006418856898 1107 58-59 0.008210992643270743 1107 60-61 -0.14867406134065675 1107 62-63 -0.058999754770013624 1107 64-65 -0.05860824440669532 1107 66-67 -0.02632733608772142 1107 68-69 0.05135709600510552 1107 70-71 0.040021831590316026 1107 72-73 0.07504159850008207 1107 74-75 0.04082808810143135 1107 76-77 0.07643269680966469 1107 78-79 0.02290784602190854 1107 80-81 0.015607060712188314 1107 82-83 -0.01950630281330845 1107 84-85 -0.022243552909973374 1107 86-87 -0.004487373954184193 1107 88-89 -0.039698503629963966 1107 90-91 -0.01863990038781438 1107 92-93 -0.09574049894738934 1107 94-95 -0.09003366546913938 1107 96-97 -0.04706152362844662 1107 98-99 -0.09881316930553652 1107 100-101 -0.17184928040595437 1108 1 -0.005286112013138222 1108 2 0.03384994865648139 1108 3 0.0811798727342854 1108 4 0.1315058618087761 1108 5 0.0019029689642167114 1108 6 -0.023737773458861966 1108 7 0.09819092022605247 1108 8 0.02622474903029115 1108 9 0.03363164779309358 1108 10-11 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0.47694154989747517 2311 42-43 0.446824438094346 2311 44-45 0.5055173466461156 2311 46-47 0.5094159170693402 2311 48-49 0.5146259702774998 2311 50-51 0.23275751805289246 2311 52-53 0.2750748856054557 2311 54-55 0.34965762049336746 2311 56-57 0.5591325548148163 2311 58-59 0.5270872703971392 2311 60-61 0.3512515276249957 2311 62-63 0.3924924211776215 2311 64-65 0.4492042373385807 2311 66-67 0.44266442092736114 2311 68-69 0.3644339686189184 2311 70-71 0.369479390063411 2311 72-73 0.497239601372673 2311 74-75 0.4274059725100585 2311 76-77 0.4234132659392067 2311 78-79 0.43161155573127985 2311 80-81 0.4588099360562552 2311 82-83 0.3976229897988013 2311 84-85 0.40156729110898226 2311 86-87 0.4561525611541235 2311 88-89 0.48341952080989614 2311 90-91 0.4486852627240303 2311 92-93 0.41394152801037976 2311 94-95 0.3384165930057925 2311 96-97 0.35376625103045 2311 98-99 0.34034921558051323 2311 100-101 0.3248893141420339 2312 1 0.254062978039606 2312 2 0.20655259216582067 2312 3 0.1886845945387634 2312 4 0.1731984220868128 2312 5 0.19268659798864007 2312 6 0.15662475572710832 2312 7 0.15224445729806746 2312 8 0.18006381197604782 2312 9 0.19789302399217945 2312 10-11 0.23541623559142266 2312 12-13 0.1588376962051683 2312 14-15 0.23723159901204127 2312 16-17 0.18835346189802493 2312 18-19 0.2332317361538827 2312 20-21 0.2501086525893186 2312 22-23 0.19027206519046302 2312 24-25 0.21620985766278267 2312 26-27 0.20808398545276674 2312 28-29 0.23309026798632004 2312 30-31 0.25526870664969437 2312 32-33 0.04254520947201712 2312 34-35 0.23937365986424908 2312 36-37 0.2854400494526388 2312 38-39 0.21928676303646455 2312 40-41 0.24091921421454998 2312 42-43 0.2420636616371752 2312 44-45 0.35475872344962767 2312 46-47 0.24767002758600398 2312 48-49 0.31194162608721143 2312 50-51 0.2140559636756052 2312 52-53 0.2871540926159568 2312 54-55 0.35227705616872385 2312 56-57 0.47938081717823167 2312 58-59 0.3093103534883781 2312 60-61 0.23409238781517416 2312 62-63 0.23880036009362726 2312 64-65 0.31652025814802087 2312 66-67 0.24719075340851404 2312 68-69 0.21799253783784422 2312 70-71 0.19431864290308454 2312 72-73 0.2176032565576591 2312 74-75 0.27261825610396073 2312 76-77 0.26566716836445536 2312 78-79 0.22928060782653503 2312 80-81 0.3253555039293623 2312 82-83 0.33510353170778373 2312 84-85 0.2601942655543894 2312 86-87 0.2429211724137872 2312 88-89 0.2553291015008057 2312 90-91 0.2623763735336162 2312 92-93 0.23789503331755313 2312 94-95 0.23009409899163913 2312 96-97 0.2952420710417627 2312 98-99 0.32849771210966594 2312 100-101 0.3225525265571072 2313 1 -0.044241990234848316 2313 2 -0.05215679342079227 2313 3 -0.06995963246388115 2313 4 -0.05031942179351745 2313 5 -0.0574054218922484 2313 6 -0.11055754869862966 2313 7 -0.04815659219484303 2313 8 -0.014876109797334891 2313 9 -0.07999045795622095 2313 10-11 -0.4825263046678643 2313 12-13 -0.1745021712334207 2313 14-15 -0.12005528388632314 2313 16-17 -0.11189057132533975 2313 18-19 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-0.45212726014266735 2314 38-39 -0.5065208784793711 2314 40-41 -0.4178327232645245 2314 42-43 -0.3616895758879082 2314 44-45 -0.37378653393438555 2314 46-47 -0.36020977553069145 2314 48-49 -0.3449492394577831 2314 50-51 -0.3403381679135862 2314 52-53 -0.2570561946559877 2314 54-55 -0.32824103171426344 2314 56-57 -0.25979692385416087 2314 58-59 -0.3653320979548056 2314 60-61 -0.4664504109656704 2314 62-63 -0.44428063788762984 2314 64-65 -0.3704782658929524 2314 66-67 -0.47637235201942474 2314 68-69 -0.4821290433722041 2314 70-71 -0.45070927093687274 2314 72-73 -0.49612858643440205 2314 74-75 -0.47167868099570853 2314 76-77 -0.37381769178713853 2314 78-79 -0.35055130111183175 2314 80-81 -0.3624508952449972 2314 82-83 -0.35308002855993337 2314 84-85 -0.38682798249456596 2314 86-87 -0.37919694892150346 2314 88-89 -0.37357522847834446 2314 90-91 -0.45994645932283973 2314 92-93 -0.47328720350255793 2314 94-95 -0.4208107195609223 2314 96-97 -0.43750598687558906 2314 98-99 -0.48687092693611334 2314 100-101 -0.5741668302834455 2315 1 -0.9989585630094595 2315 2 -0.8829420616191221 2315 3 -1.0645357763426944 2315 4 -1.1479428435063497 2315 5 -1.0826705618093442 2315 6 -1.1757752892532665 2315 7 -1.1197334100505145 2315 8 -1.0239763958244126 2315 9 -1.2126743885097042 2315 10-11 -1.3076470695463698 2315 12-13 -1.3539198072819332 2315 14-15 -1.6609915216696933 2315 16-17 -1.6873348193372308 2315 18-19 -1.6031771666523156 2315 20-21 -1.68479047496713 2315 22-23 -1.7301198303847798 2315 24-25 -1.768294141927285 2315 26-27 -1.836742169638093 2315 28-29 -1.8887158471000376 2315 30-31 -1.8666437884638256 2315 32-33 -1.7601894840756884 2315 34-35 -1.667225413260212 2315 36-37 -1.638893804228239 2315 38-39 -1.5729054573697923 2315 40-41 -1.6160280695839475 2315 42-43 -1.7068871477360794 2315 44-45 -1.6277639019796837 2315 46-47 -1.678922646102457 2315 48-49 -1.735023686127036 2315 50-51 -1.725505835879595 2315 52-53 -1.6610144850294333 2315 54-55 -1.6704897013978126 2315 56-57 -1.5877863177185851 2315 58-59 -1.594517902418037 2315 60-61 -1.7447370627072587 2315 62-63 -1.731076673016748 2315 64-65 -1.6853326464310676 2315 66-67 -1.6683121128804927 2315 68-69 -1.7733900670749314 2315 70-71 -1.7865138546776436 2315 72-73 -1.757829690773594 2315 74-75 -1.8013226383006682 2315 76-77 -1.7778104890398048 2315 78-79 -1.8304111306372022 2315 80-81 -1.7649596569443808 2315 82-83 -1.793877262863024 2315 84-85 -1.880234330888257 2315 86-87 -1.8790452355036606 2315 88-89 -1.8851410518862082 2315 90-91 -1.8875236568850369 2315 92-93 -1.912433560994696 2315 94-95 -1.9981962012006242 2315 96-97 -2.3621013923454086 2315 98-99 -2.401885053746849 2315 100-101 -2.4274718429112667 2316 1 -2.9428022327180905 2316 2 -3.2383316520483056 2316 3 -3.1328918058685176 2316 4 -3.4280996863063073 2316 5 -3.338816971371994 2316 6 -3.43347225165385 2316 7 -3.25104461980872 2316 8 -3.352681619960819 2316 9 -3.9229071006162144 2316 10-11 -4.145244803298738 2316 12-13 -4.0687618714686735 2316 14-15 -4.669311127921269 2316 16-17 -4.395184499500083 2316 18-19 -4.470566243490147 2316 20-21 -4.545036621480762 2316 22-23 -4.58222784902765 2316 24-25 -4.448351146447919 2316 26-27 -4.395180383203307 2316 28-29 -4.617513438991232 2316 30-31 -4.608414981247407 2316 32-33 -4.550431511030492 2316 34-35 -4.65396708215269 2316 36-37 -4.5731987811200305 2316 38-39 -4.477996376339995 2316 40-41 -4.557073273026674 2316 42-43 -4.757940676994796 2316 44-45 -4.616634681997315 2316 46-47 -4.707731768242525 2316 48-49 -4.705034147655553 2316 50-51 -4.617975594383161 2316 52-53 -4.557376230692718 2316 54-55 -4.755990737680261 2316 56-57 -4.71776908884781 2316 58-59 -4.815086618778256 2316 60-61 -5.057570882100023 2316 62-63 -4.999815366048907 2316 64-65 -4.950176282212045 2316 66-67 -4.890216394913562 2316 68-69 -4.793597312928036 2316 70-71 -4.8182967383335935 2316 72-73 -4.76308008941567 2316 74-75 -4.723327253061619 2316 76-77 -4.7573450096104715 2316 78-79 -4.729566950414924 2316 80-81 -4.496125712367519 2316 82-83 -4.57809871067229 2316 84-85 -4.668801731987585 2316 86-87 -4.676035360198611 2316 88-89 -4.722405937675028 2316 90-91 -4.753432150908726 2316 92-93 -4.721997579238565 2316 94-95 -4.87236101267608 2316 96-97 -5.0030131782454745 2316 98-99 -5.0863301248966195 2316 100-101 -5.305338293565736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 894143.0 3 246872.0 4 226971.0 5 243155.0 6 306581.0 7 464866.0 8 754525.0 9 1197654.0 10 1313430.0 11 944604.0 12 627074.0 13 491037.0 14 423610.0 15 377049.0 16 351453.0 17 338593.0 18 340422.0 19 354481.0 20 379924.0 21 415740.0 22 461405.0 23 517640.0 24 586246.0 25 610645.0 26 643837.0 27 720371.0 28 819087.0 29 941906.0 30 1100991.0 31 1307824.0 32 1591157.0 33 1981650.0 34 2580230.0 35 3599885.0 36 5498816.0 37 9984785.0 38 1.5417627E7 39 5065175.0 40 16401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.167411327998174 18.509987833811255 9.133744067515346 46.18885677067522 2 12.135585609102327 16.2902766638876 60.54065666731031 11.033481059699755 3 15.131698469586158 23.39342737578494 40.123198151531945 21.351676003096962 4 17.623926244988812 29.91669148089167 34.58204427658805 17.87733799753147 5 17.993823876220713 32.60999675637423 34.69207941755032 14.70409994985474 6 14.308831582175907 35.56821298349347 35.43726919059645 14.685686243734168 7 12.321761409348358 19.520526755961217 52.90420457846571 15.253507256224715 8 12.435160363655303 21.25470028845381 46.45221177206097 19.857927575829915 9 13.863051934824641 23.050673233893598 43.61375126418718 19.472523567094584 10-11 19.073741661943664 31.767543732538943 33.31579940369176 15.842915201825628 12-13 17.698982635189832 24.87154502274518 39.664828071624555 17.764644270440424 14-15 18.0418779942247 26.916720866303628 38.74441431855116 16.296986820920516 16-17 18.900816002016725 26.45662785631532 37.58327404405403 17.059282097613927 18-19 18.531550787044893 28.014913977590407 36.43262262804215 17.020912607322554 20-21 19.427846355169905 27.599359060094802 35.410871966266534 17.56192261846876 22-23 19.939622446669265 28.859188421968152 34.129402465832065 17.07178666553052 24-25 18.468746099351353 29.81344006364531 33.562299732859216 18.15551410414412 26-27 18.501844154931582 30.11520852253482 33.428532107620704 17.954415214912885 28-29 18.54797298328833 30.633424151464013 32.573908998382315 18.24469386686535 30-31 18.373541966102096 30.92053522872738 32.38169276181345 18.32423004335708 32-33 18.527540904684102 30.10855637429475 32.6876406476485 18.676262073372655 34-35 19.5111725961499 31.017275162566825 31.005202733521553 18.46634950776172 36-37 18.894665520445283 31.12115604845855 31.665818916683676 18.318359514412496 38-39 19.03319419741287 29.884031187707144 32.557197076388675 18.52557753849131 40-41 19.698223426503304 29.84286134024118 31.888893349375575 18.570021883879946 42-43 19.701980723026065 29.82372109571215 32.302826517968505 18.171471663293286 44-45 19.982303536655404 29.62456159798193 31.723485128942535 18.669649736420133 46-47 19.100103285337372 29.52775557020688 32.44654423522401 18.92559690923173 48-49 19.055988412760403 29.8537351680899 31.43080181240962 19.659474606740076 50-51 18.32291718898923 30.063278081768154 32.76730366443625 18.84650106480637 52-53 19.473078307033436 30.301995050199633 31.495575047800774 18.72935159496616 54-55 18.777864079836856 30.77282848264397 30.616026800275808 19.833280637243362 56-57 19.683297131646082 30.551691168013313 30.909805542642992 18.85520615769761 58-59 19.24093553429461 31.14174540597056 30.87010766147651 18.74721139825832 60-61 19.269169753384872 31.8822099158098 29.72912235979073 19.119497971014603 62-63 19.53585486441719 31.440490873700416 30.609297756074948 18.414356505807447 64-65 19.92890589829602 30.449533925110174 30.59485205261767 19.026708123976135 66-67 20.01273777159338 31.907531929507137 28.993862249037587 19.085868049861897 68-69 19.830229386416782 30.97926827017804 30.525731224385066 18.664771119020106 70-71 19.793797121613625 30.807119788412557 29.970961048386712 19.428122041587105 72-73 19.122425600700293 31.74893818998311 29.671194959301772 19.457441250014824 74-75 19.112470088929538 30.377643204624967 31.327044789672115 19.18284191677338 76-77 19.112917169696146 31.04095027379469 30.451423937908167 19.394708618600998 78-79 19.29029196147935 31.639868663595333 29.963641069765018 19.106198305160298 80-81 19.513221617401513 30.733369267525774 30.424283710788664 19.32912540428405 82-83 20.075705168597576 31.256229354775165 29.44114769983083 19.22691777679643 84-85 19.36213934841251 30.803038855121073 30.116153820190007 19.718667976276407 86-87 19.77576731002131 31.353986961272824 29.76840724922679 19.10183847947908 88-89 19.619947392298513 30.629231890587917 30.488421264182104 19.26239945293147 90-91 19.523083374365292 31.364433802280047 30.34745464885657 18.765028174498095 92-93 20.003982063433806 30.687047763047104 30.16702936709731 19.14194080642178 94-95 19.5157566267479 30.755961064773864 30.446010506442388 19.282271802035847 96-97 19.391982190084367 31.104442628021705 29.93840765084313 19.565167531050797 98-99 19.858763294630148 30.54111075156897 30.333438411920525 19.266687541880362 100-101 20.32727947860944 31.686746220438078 28.809832082202174 19.176142218750307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23106.0 1 36930.0 2 43629.0 3 39686.5 4 47187.5 5 57320.5 6 67855.0 7 79403.0 8 93119.0 9 107198.0 10 121195.0 11 135196.5 12 151119.5 13 166008.5 14 182007.5 15 199990.0 16 216238.0 17 233700.5 18 260817.0 19 290106.0 20 333448.5 21 389235.5 22 437979.0 23 505959.0 24 643708.0 25 774766.0 26 873387.0 27 1051060.0 28 1330275.5 29 1570704.5 30 1802427.0 31 1993602.0 32 2402142.5 33 2731420.0 34 2637467.5 35 2782271.0 36 2953858.0 37 3184074.0 38 3395561.5 39 3108199.0 40 2722823.5 41 2436258.5 42 2143078.0 43 1920932.5 44 1757905.0 45 1646000.5 46 1572870.5 47 1485205.5 48 1399405.0 49 1391881.5 50 1243395.5 51 974807.5 52 875191.0 53 815145.0 54 725201.5 55 641354.5 56 566442.5 57 474745.0 58 392226.0 59 326325.5 60 253928.0 61 187485.0 62 147923.0 63 114506.0 64 78662.0 65 56766.0 66 44120.0 67 36029.5 68 28161.5 69 20665.0 70 18193.0 71 22521.5 72 21995.0 73 30797.0 74 33597.0 75 20264.0 76 15138.5 77 16764.0 78 15984.0 79 9522.5 80 5505.5 81 2979.0 82 1605.0 83 785.0 84 364.0 85 197.5 86 133.0 87 91.5 88 56.0 89 35.0 90 25.0 91 17.0 92 14.5 93 11.0 94 9.0 95 7.0 96 3.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6706709369264601 2 0.31226485223345923 3 0.3103907641947903 4 0.3475482235438406 5 0.3361010069216214 6 0.17687680328352695 7 0.16066640949147945 8 0.16924012839717045 9 0.1677464708755025 10-11 0.17684328174206992 12-13 0.192261631670853 14-15 0.18455245670646148 16-17 0.17038687694329444 18-19 0.1855245814087161 20-21 0.21337474579367802 22-23 0.1693274403191051 24-25 0.18407380027728396 26-27 0.1740820422108863 28-29 0.17106042605536181 30-31 0.19006480134931844 32-33 0.14475615043108236 34-35 0.17432370913766973 36-37 0.16620681868067258 38-39 0.16861647181192288 40-41 0.16602283998802453 42-43 0.13821711113476157 44-45 0.5016085506560851 46-47 0.1305866728142575 48-49 0.10828783160873058 50-51 0.11077622138386839 52-53 0.5574117827625747 54-55 0.38654624939010285 56-57 0.2788158108544373 58-59 0.2334034770289038 60-61 0.06592050106066835 62-63 0.06959072006485031 64-65 0.06806587971391999 66-67 0.07753454581944125 68-69 0.09297862158236582 70-71 0.10261879324883014 72-73 0.13560399004257423 74-75 0.10853183724770869 76-77 0.09655607166949219 78-79 0.10394796134613904 80-81 0.09728964772788966 82-83 0.08212543785759495 84-85 0.08520552181798638 86-87 0.09925962296654042 88-89 0.09292716991408288 90-91 0.11217555084701762 92-93 0.12178298054275648 94-95 0.1416838621780065 96-97 0.10521944120931252 98-99 0.12883107952678557 100-101 0.14232778760227463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.4137862E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.68650869646848 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36954388480984 16.832517772007925 2 7.35586863169104 3.043344808391128 3 2.8948783022432707 1.7965477552372 4 1.6043909201513378 1.3275698688898285 5 0.9877565040454176 1.0216616755464418 6 0.7151967087831442 0.8876953761556888 7 0.5453033140361614 0.7896295223615495 8 0.4300422878328345 0.7116858821682506 9 0.3471357397685177 0.6462923849618815 >10 2.837607881145306 12.18272407692233 >50 0.4416749421622298 6.420833224357141 >100 0.38588388104743165 16.27758620527064 >500 0.046869223383078126 6.701003051139527 >1k 0.0319979116181645 12.873817524195033 >5k 0.003373449646272122 4.791568461575873 >10k+ 0.002476417636066116 13.69552241081956 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 204520 0.3188756120370835 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 202961 0.31644491049608103 Illumina Single End PCR Primer 1 (100% over 50bp) CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 191992 0.2993426877871296 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 143511 0.22375395051366073 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 135486 0.2112418402721313 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 131654 0.20526721018545957 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115350 0.17984696777076853 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 114863 0.1790876658782296 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 88525 0.13802299802260326 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 80692 0.12581024294199267 No Hit AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT 79443 0.12386287525455714 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 76841 0.11980598916752168 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 73551 0.1146764137538604 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 72353 0.11280856228104391 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 70258 0.10954216091580975 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 65286 0.10179010956118244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.4197380324277102 0.0 0.0 1.5591414631189298E-6 0.0 2 0.4207888937738523 0.0 0.0 1.5591414631189298E-6 0.0 3 0.4209245390811437 0.0 0.0 1.5591414631189298E-6 0.0 4 0.4287670206406319 0.0 0.0 1.5591414631189298E-6 0.0 5 0.42919890282591583 0.0 0.0 3.1182829262378595E-6 0.0 6 0.42981008627945844 0.0 0.0 4.677424389356789E-6 0.0 7 0.4303885277622756 0.0 0.0 6.236565852475719E-6 0.0 8 0.4341024027274249 0.0 0.0 7.795707315594649E-6 0.0 9 0.43442982243467987 0.0 0.0 9.354848778713577E-6 0.0 10-11 0.43664302374157715 0.0 0.0 9.354848778713577E-6 0.0 12-13 0.43907840270696896 0.0 0.0 9.354848778713577E-6 0.0 14-15 0.441039023096841 0.0 0.0 9.354848778713577E-6 0.0 16-17 0.44354456342807314 0.0 0.0 9.354848778713577E-6 0.0 18-19 0.44588639390567775 0.0 0.0 1.3252702436510902E-5 0.0 20-21 0.4483358051442376 7.795707315594649E-7 0.0 1.7150556094308226E-5 0.0 22-23 0.4507134958754939 1.5591414631189298E-6 0.0 1.7150556094308226E-5 0.0 24-25 0.45277078303607937 1.5591414631189298E-6 0.0 2.3387121946783944E-5 0.0 26-27 0.4547875325186237 3.1182829262378595E-6 0.0 3.040325853081913E-5 0.0 28-29 0.4567005990938706 4.677424389356789E-6 0.0 3.3521541457056986E-5 0.0 30-31 0.4587352787032408 7.016136584035184E-6 0.0 3.741939511485431E-5 0.0 32-33 0.4608440175321092 1.0913990241832508E-5 0.0 3.741939511485431E-5 0.0 34-35 0.46447603757044476 1.2473131704951438E-5 0.0 3.741939511485431E-5 0.0 36-37 0.4668942659797422 1.2473131704951438E-5 0.0 4.0537678041092173E-5 0.0 38-39 0.46928053198904573 1.4032273168070367E-5 0.0 4.131724877265163E-5 0.0 40-41 0.4717962067397881 1.4032273168070367E-5 0.0 4.2876390235770565E-5 0.0 42-43 0.4746018818026706 1.4032273168070367E-5 1.5591414631189298E-6 4.365596096733003E-5 0.0 44-45 0.4778371003386424 1.4032273168070367E-5 3.1182829262378595E-6 4.365596096733003E-5 0.0 46-47 0.48312492861081024 1.4032273168070367E-5 3.1182829262378595E-6 4.833338535668682E-5 0.0 48-49 0.4866587227369693 1.5591414631189297E-5 3.1182829262378595E-6 4.833338535668682E-5 1.5591414631189298E-6 50-51 0.49039910310699164 1.5591414631189297E-5 3.1182829262378595E-6 4.989252681980575E-5 1.5591414631189298E-6 52-53 0.4945183548525518 1.5591414631189297E-5 3.1182829262378595E-6 4.989252681980575E-5 3.1182829262378595E-6 54-55 0.499069488783396 1.637098536274876E-5 4.677424389356789E-6 5.145166828292468E-5 3.1182829262378595E-6 56-57 0.5040743328800078 1.8709697557427155E-5 4.677424389356789E-6 5.3790380477603076E-5 3.1182829262378595E-6 58-59 0.5092803062253619 1.8709697557427155E-5 4.677424389356789E-6 5.76882341354004E-5 3.1182829262378595E-6 60-61 0.5153165223998268 1.948926828898662E-5 4.677424389356789E-6 6.002694633007879E-5 3.1182829262378595E-6 62-63 0.5220956694814679 2.0268839020546087E-5 4.677424389356789E-6 6.236565852475719E-5 3.1182829262378595E-6 64-65 0.5295280968361558 2.0268839020546087E-5 4.677424389356789E-6 6.314522925631665E-5 3.1182829262378595E-6 66-67 0.5381111705906256 2.104840975210555E-5 4.677424389356789E-6 6.548394145099504E-5 3.1182829262378595E-6 68-69 0.5475564495742 2.260755121522448E-5 4.677424389356789E-6 6.62635121825545E-5 3.1182829262378595E-6 70-71 0.55814925667463 2.416669267834341E-5 4.677424389356789E-6 6.938179510879237E-5 3.1182829262378595E-6 72-73 0.5695676291797815 2.6505404873021805E-5 4.677424389356789E-6 7.016136584035184E-5 3.1182829262378595E-6 74-75 0.5831267029137952 2.6505404873021805E-5 4.677424389356789E-6 7.32796487665897E-5 3.1182829262378595E-6 76-77 0.5983790042767563 2.6505404873021805E-5 4.677424389356789E-6 8.575278047154113E-5 3.1182829262378595E-6 78-79 0.6155630819125215 2.6505404873021805E-5 4.677424389356789E-6 9.120977559245739E-5 3.1182829262378595E-6 80-81 0.6358210069428258 2.8844117067700196E-5 4.677424389356789E-6 9.900548290805203E-5 3.1182829262378595E-6 82-83 0.6589539888311213 2.9623687799259662E-5 7.795707315594649E-6 1.0446247802896829E-4 3.1182829262378595E-6 84-85 0.6871261159282173 2.9623687799259662E-5 7.795707315594649E-6 1.0446247802896829E-4 3.1182829262378595E-6 86-87 0.7195546992196279 3.040325853081913E-5 7.795707315594649E-6 1.0913990241832507E-4 3.897853657797324E-6 88-89 0.7577294983733633 3.1182829262378594E-5 7.795707315594649E-6 1.0991947314988454E-4 4.677424389356789E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTTTT 944740 0.0 50.75116 1 CGCGTGT 35315 0.0 50.673195 1 GCGTGTT 43690 0.0 41.00312 2 AGAGCGT 65205 0.0 39.95991 9 CGCCGTA 39885 0.0 39.927967 46-47 CCGTATC 40940 0.0 39.26712 48-49 AAGAGCG 68365 0.0 39.105576 8 GTCGCCG 40995 0.0 38.081287 44-45 AGATCGG 72120 0.0 36.905666 1 TCCGATC 7030 0.0 36.01622 1 ATCGGAA 75920 0.0 35.260166 3 CGGAAGA 76765 0.0 34.667088 5 TCGGTGG 46275 0.0 34.663403 38-39 GATCGGA 77150 0.0 34.62984 2 ATCAATA 57585 0.0 34.34073 4 TGGTCGC 42795 0.0 34.206184 42-43 CCGATCT 7570 0.0 34.093906 2 GATCTCG 51215 0.0 33.4863 34-35 CCTTTAG 43240 0.0 33.427265 5 TCGTACC 14580 0.0 32.958427 2 >>END_MODULE