FastQCFastQC Report
Fri 27 May 2016
SRR1233083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64137862
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2195420.3422970350960561TruSeq Adapter, Index 6 (100% over 49bp)
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1835650.28620380267742634No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1719990.2681707725149928No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1408870.21966276331443663No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1299380.2025917234347475No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1108020.17275599239650363No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1083230.16889088070943184No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT948240.14784403009878938No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC946710.14760548145493219TruSeq Adapter, Index 6 (100% over 50bp)
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA916200.14284854085095633No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT875580.13651530822776722No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA750860.11706969589974794No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT732270.11417125191980985No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT668290.10419586483877495No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC667830.10412414433147149No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT660300.10295011080974292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT371650.052.6154981
GTTTTTT8632900.047.5471571
GCGTGTT448500.044.2112772
TATGCCG517100.037.1316246-47
AGATCGG693850.037.063741
TGCCGTC505200.036.83000648-49
ACGCCAA556350.035.7090832-33
CCGTCTT536450.034.88693250-51
TCACGCC565600.034.40725330-31
CTCGTAT554500.034.1838942-43
ATCAATA585350.033.8135074
ATCGGAA783700.033.423913
CGGAAGA805100.033.342285
GATCGGA791150.033.149662
AGTCACG594500.032.397328-29
CGTCTGA622300.031.95960616-17
CATCAAT614850.031.9038413
ATCTCGT582850.031.88489740-41
TCGTACC145550.031.3682212
TCGGAAG859800.030.7045944
Produced by FastQC (version 0.11.5)