##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64137862 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.996334723474256 31.0 30.0 34.0 25.0 34.0 2 30.893790971704046 33.0 31.0 34.0 27.0 34.0 3 30.76966960638632 34.0 31.0 34.0 26.0 34.0 4 34.10466574330152 37.0 35.0 37.0 30.0 37.0 5 33.98894632627449 37.0 35.0 37.0 29.0 37.0 6 34.154578523368926 37.0 35.0 37.0 30.0 37.0 7 34.279398633524764 37.0 35.0 37.0 31.0 37.0 8 34.259159932708705 37.0 35.0 37.0 31.0 37.0 9 35.810052804691246 39.0 37.0 39.0 31.0 39.0 10-11 35.62658438006555 39.0 37.0 39.0 30.0 39.0 12-13 35.57991519891948 39.0 37.0 39.0 30.0 39.0 14-15 36.8185079898672 40.0 37.0 41.0 30.0 41.0 16-17 36.62963571345736 40.0 37.0 41.0 30.0 41.0 18-19 36.23061513961909 40.0 36.5 41.0 28.5 41.0 20-21 35.86702228240785 40.0 36.0 41.0 27.0 41.0 22-23 35.58407008484318 40.0 35.5 41.0 26.0 41.0 24-25 35.21764376866818 40.0 35.0 41.0 22.5 41.0 26-27 34.76685911357632 40.0 35.0 41.0 16.0 41.0 28-29 34.41457343869679 40.0 35.0 41.0 9.0 41.0 30-31 34.14159010320613 40.0 35.0 41.0 4.5 41.0 32-33 33.897562831140206 40.0 34.5 41.0 2.0 41.0 34-35 33.73970631418927 40.0 34.0 41.0 2.0 41.0 36-37 33.796558333983754 40.0 35.0 41.0 2.0 41.0 38-39 33.76223965027085 40.0 35.0 41.0 2.0 41.0 40-41 33.66263625999881 40.0 34.5 41.0 2.0 41.0 42-43 33.551669324431174 40.0 34.0 41.0 2.0 41.0 44-45 33.45734528849745 40.0 34.0 41.0 2.0 41.0 46-47 33.332340560400965 40.0 34.0 41.0 2.0 41.0 48-49 33.245367884261555 40.0 34.0 41.0 2.0 41.0 50-51 33.14839834386747 39.0 33.0 41.0 2.0 41.0 52-53 32.88779991606206 39.0 33.0 41.0 2.0 41.0 54-55 32.771386478395556 39.0 33.0 41.0 2.0 41.0 56-57 32.65965869738533 39.0 33.0 41.0 2.0 41.0 58-59 32.46432887800344 39.0 33.0 41.0 2.0 41.0 60-61 32.410108236847684 39.0 33.0 41.0 2.0 41.0 62-63 32.26411030040259 38.5 32.5 41.0 2.0 41.0 64-65 31.898984791541693 38.0 32.0 40.0 2.0 41.0 66-67 31.699467500179537 37.5 32.0 40.0 2.0 41.0 68-69 31.235616662744384 37.0 31.0 40.0 2.0 41.0 70-71 30.917582534946362 36.5 31.0 39.0 2.0 41.0 72-73 30.604723626428335 36.0 31.0 39.0 2.0 41.0 74-75 30.267148568188944 35.5 30.5 39.0 2.0 40.5 76-77 29.16244963076568 34.5 28.5 37.0 2.0 39.0 78-79 29.57599034093154 35.0 30.0 37.0 2.0 39.0 80-81 29.389994836435303 35.0 30.0 37.0 2.0 39.0 82-83 29.09375144123139 35.0 30.0 36.5 2.0 39.0 84-85 28.787845110895653 35.0 30.0 36.0 2.0 37.5 86-87 28.46883242693684 35.0 29.0 36.0 2.0 37.0 88-89 28.214191813877427 35.0 29.0 35.0 2.0 37.0 90-91 27.963362732608704 35.0 29.0 35.0 2.0 36.0 92-93 27.724448010131674 34.5 29.0 35.0 2.0 36.0 94-95 27.483956870904116 34.0 29.0 35.0 2.0 36.0 96-97 27.255587869455333 34.0 27.5 35.0 2.0 36.0 98-99 26.97131956939881 34.0 27.0 35.0 2.0 35.0 100-101 25.980486206415797 33.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2024827507515887 1101 2 0.6762087589051724 1101 3 0.8802638394938853 1101 4 1.0942675725482331 1101 5 1.0517745027066852 1101 6 0.8639640020253694 1101 7 0.746859384105484 1101 8 0.7651674166400397 1101 9 0.9011997017971964 1101 10-11 0.95488748763416 1101 12-13 0.9263932958552417 1101 14-15 1.0101350999072665 1101 16-17 0.9791218186630175 1101 18-19 0.9582842899713313 1101 20-21 0.9696761009590347 1101 22-23 0.8756797416458539 1101 24-25 0.9448693306465117 1101 26-27 0.8795453100231398 1101 28-29 0.8725861040675085 1101 30-31 0.9206711989090053 1101 32-33 0.9405529652608706 1101 34-35 0.9266515053738971 1101 36-37 0.8519070468936434 1101 38-39 0.887017043952973 1101 40-41 0.8570991267598913 1101 42-43 0.8796533083895568 1101 44-45 0.8946381577218929 1101 46-47 0.8717024670711453 1101 48-49 0.8626147965556683 1101 50-51 0.8632235487653688 1101 52-53 0.9905999494909992 1101 54-55 0.9616065255719519 1101 56-57 0.8848673013804884 1101 58-59 0.8273280841188608 1101 60-61 0.7504901831085604 1101 62-63 0.7827729775745027 1101 64-65 0.7757193090605838 1101 66-67 0.8277204485602354 1101 68-69 0.6696244204028368 1101 70-71 0.8436726183524925 1101 72-73 0.9259090676884796 1101 74-75 0.8345649713278149 1101 76-77 0.7459058473126312 1101 78-79 0.7241146073822833 1101 80-81 0.7162057980799617 1101 82-83 0.7981923530704016 1101 84-85 0.9357023001063673 1101 86-87 0.940351535162602 1101 88-89 0.9453484424137812 1101 90-91 0.9250089248556002 1101 92-93 0.9117541699796412 1101 94-95 0.8716833350453008 1101 96-97 0.9020034364672007 1101 98-99 0.9753637529614991 1101 100-101 1.0248030067303944 1102 1 1.465068432518386 1102 2 0.9254495950569073 1102 3 1.066111537580209 1102 4 1.0992743642718992 1102 5 1.0025234804886693 1102 6 0.7790761196895062 1102 7 0.6943732163511029 1102 8 0.6714994044355791 1102 9 0.7934985103976118 1102 10-11 0.8435344613585585 1102 12-13 0.8022915724548056 1102 14-15 0.8808690210902554 1102 16-17 0.770811397374402 1102 18-19 0.8304266139521275 1102 20-21 0.8384358401020364 1102 22-23 0.8619148299811101 1102 24-25 0.8586001087261934 1102 26-27 0.8406692840301844 1102 28-29 0.8978299952852637 1102 30-31 0.8980107738651029 1102 32-33 0.8446408368772822 1102 34-35 0.8619407499874185 1102 36-37 0.8346839344368178 1102 38-39 0.8540703283731617 1102 40-41 0.8772830312918387 1102 42-43 0.8299982416746445 1102 44-45 0.8978475964675141 1102 46-47 0.8823968345239521 1102 48-49 0.8619888937531286 1102 50-51 0.8361124090882726 1102 52-53 0.9703153344314899 1102 54-55 0.89866337761012 1102 56-57 0.8289659807523364 1102 58-59 0.7906976784209476 1102 60-61 0.8488656486395314 1102 62-63 0.8110972592248302 1102 64-65 0.8331209493725638 1102 66-67 0.8358849244546889 1102 68-69 0.6903868387230112 1102 70-71 0.8019537592159303 1102 72-73 0.8198636209819483 1102 74-75 0.8264383768041128 1102 76-77 0.7512191395018704 1102 78-79 0.8177556034902871 1102 80-81 0.8281048000089264 1102 82-83 0.799248057901842 1102 84-85 0.7737212507080322 1102 86-87 0.6966975119610233 1102 88-89 0.7101563400695383 1102 90-91 0.7292649335561485 1102 92-93 0.7394953902167423 1102 94-95 0.7589664338436073 1102 96-97 0.7584909772728246 1102 98-99 0.7664462578041267 1102 100-101 0.7282571931639517 1103 1 1.4434189875968855 1103 2 0.8893260663871523 1103 3 1.0807490835404003 1103 4 1.0507352010954207 1103 5 0.9825909684947973 1103 6 0.7968302998306172 1103 7 0.7020132903110508 1103 8 0.6273358980365131 1103 9 0.7085919442328361 1103 10-11 0.7703112519306998 1103 12-13 0.7629347751135427 1103 14-15 0.7897558733419885 1103 16-17 0.7620678522053907 1103 18-19 0.7557292823827382 1103 20-21 0.735092201011966 1103 22-23 0.7813304455423378 1103 24-25 0.8102468647747045 1103 26-27 0.7862226313513148 1103 28-29 0.7970860810259381 1103 30-31 0.773719743810311 1103 32-33 0.7721791499826693 1103 34-35 0.7871469090119021 1103 36-37 0.7486735673491864 1103 38-39 0.747993073834273 1103 40-41 0.6952748905632546 1103 42-43 0.7378849169904456 1103 44-45 0.7230527321249554 1103 46-47 0.7690845198537843 1103 48-49 0.7486406323495132 1103 50-51 0.742813692074705 1103 52-53 0.8621273656074777 1103 54-55 0.7678209812094821 1103 56-57 0.7071622928607866 1103 58-59 0.7389151859049221 1103 60-61 0.773407535419814 1103 62-63 0.7539062042514004 1103 64-65 0.7889115754156464 1103 66-67 0.736997490776389 1103 68-69 0.6040805544372212 1103 70-71 0.7230323897671447 1103 72-73 0.6408630508184103 1103 74-75 0.6661393538605438 1103 76-77 0.624520548508233 1103 78-79 0.7368759741119639 1103 80-81 0.7817930705235128 1103 82-83 0.7245468936077657 1103 84-85 0.6798821283074794 1103 86-87 0.6573121791662935 1103 88-89 0.6195845027993059 1103 90-91 0.5786874830721125 1103 92-93 0.5468958317624235 1103 94-95 0.5639566558355966 1103 96-97 0.5784330336954788 1103 98-99 0.5767662255176731 1103 100-101 0.5230752198043511 1104 1 1.3005310659425895 1104 2 0.8061919251970231 1104 3 0.941051792240895 1104 4 0.9794956821204366 1104 5 0.8767430407172441 1104 6 0.6770852764013569 1104 7 0.5473849335670167 1104 8 0.550756485230437 1104 9 0.5495883196614173 1104 10-11 0.6219258467485886 1104 12-13 0.5945331801951639 1104 14-15 0.6727875442603377 1104 16-17 0.5641474204711301 1104 18-19 0.5783926932667711 1104 20-21 0.6044723752433754 1104 22-23 0.6509552803022629 1104 24-25 0.6218766309059802 1104 26-27 0.6571720215776651 1104 28-29 0.6676205223341753 1104 30-31 0.6681236228282614 1104 32-33 0.6216040048085247 1104 34-35 0.5984309202388189 1104 36-37 0.5918926345879569 1104 38-39 0.6104019774730531 1104 40-41 0.5488724138215204 1104 42-43 0.5950468725959581 1104 44-45 0.5967285531618387 1104 46-47 0.6182303799394262 1104 48-49 0.5713323688101966 1104 50-51 0.5457716285392564 1104 52-53 0.5200948789288162 1104 54-55 0.5109238860379506 1104 56-57 0.5779236158942496 1104 58-59 0.6097622845161723 1104 60-61 0.5949225160608975 1104 62-63 0.6213360169599511 1104 64-65 0.6297201423166854 1104 66-67 0.601348644253207 1104 68-69 0.48036188126567936 1104 70-71 0.5751935560344812 1104 72-73 0.583590012031955 1104 74-75 0.5701527449528072 1104 76-77 0.5005385746589148 1104 78-79 0.5985355609854643 1104 80-81 0.6360475926218534 1104 82-83 0.5856027076467747 1104 84-85 0.5889815727667695 1104 86-87 0.5660757672481189 1104 88-89 0.5644567394448785 1104 90-91 0.5230158104977285 1104 92-93 0.5726330005621207 1104 94-95 0.5812254477251955 1104 96-97 0.5374232993785419 1104 98-99 0.548451155158503 1104 100-101 0.6168554754939528 1105 1 1.15015153824492 1105 2 0.6655877499573464 1105 3 0.8325048644419688 1105 4 0.8823875975819249 1105 5 0.7445034969652724 1105 6 0.49569086497391623 1105 7 0.43073737217125796 1105 8 0.42629243784809745 1105 9 0.4786437584998282 1105 10-11 0.4811344503966879 1105 12-13 0.4379981918260327 1105 14-15 0.47519219410703784 1105 16-17 0.4069425437660428 1105 18-19 0.4162548845916376 1105 20-21 0.3711265227749365 1105 22-23 0.3749514840944954 1105 24-25 0.3730338649553744 1105 26-27 0.32419766636250813 1105 28-29 0.3519002338022261 1105 30-31 0.3961776293190624 1105 32-33 0.43972685571487347 1105 34-35 0.42368450023877813 1105 36-37 0.35879408039023986 1105 38-39 0.3744060174150192 1105 40-41 0.3605339664897187 1105 42-43 0.29061540222377147 1105 44-45 0.3183830711417741 1105 46-47 0.33451491142803746 1105 48-49 0.38844002385528853 1105 50-51 0.39261421531581675 1105 52-53 0.49784329004849326 1105 54-55 0.4776617102300591 1105 56-57 0.40059197929740975 1105 58-59 0.42064619003600257 1105 60-61 0.3586673600739587 1105 62-63 0.33430114930550303 1105 64-65 0.3517320324842146 1105 66-67 0.286973344346265 1105 68-69 0.18744073508163694 1105 70-71 0.23680402665499045 1105 72-73 0.32316634009665535 1105 74-75 0.28126443949662416 1105 76-77 0.2212390155352182 1105 78-79 0.31469772436318166 1105 80-81 0.34013781833853685 1105 82-83 0.29699152138117313 1105 84-85 0.3470170668433674 1105 86-87 0.34890776182263394 1105 88-89 0.22970774783473047 1105 90-91 0.31556362016702977 1105 92-93 0.39578731586712124 1105 94-95 0.4341284112608861 1105 96-97 0.35113106167097996 1105 98-99 0.26273180663019247 1105 100-101 0.24968964175516817 1106 1 0.851637786962101 1106 2 0.34133293024708067 1106 3 0.48996800495412174 1106 4 0.5798632345472328 1106 5 0.3825181934738495 1106 6 0.21817984230581544 1106 7 0.057559522252752515 1106 8 -0.022545079686068448 1106 9 -0.06925585433127424 1106 10-11 -0.022831722996400572 1106 12-13 -0.09104643409078506 1106 14-15 -0.1223744413145198 1106 16-17 -0.1333199503141742 1106 18-19 -0.14313564423303404 1106 20-21 -0.08805692827215239 1106 22-23 -0.002417990747062504 1106 24-25 0.006358709872436918 1106 26-27 0.01494140068892591 1106 28-29 -0.054956781444815306 1106 30-31 -0.13025470635611924 1106 32-33 -0.020329465236947897 1106 34-35 -0.0982135859209805 1106 36-37 -0.06662281065860753 1106 38-39 -0.10479860054208956 1106 40-41 -0.06674050680227595 1106 42-43 -0.06344046896190747 1106 44-45 -0.04675968658710161 1106 46-47 -0.06187131072299934 1106 48-49 -0.015083229485050254 1106 50-51 3.2507957314464875E-5 1106 52-53 0.04840416454644725 1106 54-55 0.022642002375633297 1106 56-57 -0.013597979762835166 1106 58-59 -0.10136198727944645 1106 60-61 -0.09352512021532533 1106 62-63 -0.05226301606997197 1106 64-65 -0.004561735834450076 1106 66-67 -0.06169039161538237 1106 68-69 -0.14005630418290238 1106 70-71 -0.05015524749918043 1106 72-73 -0.016991943480935134 1106 74-75 -0.08581408414077885 1106 76-77 -0.18073614664118764 1106 78-79 -0.003837165881012794 1106 80-81 0.012651748407609631 1106 82-83 -0.14537230048114935 1106 84-85 -0.06278076532154486 1106 86-87 -0.04764478186689658 1106 88-89 -0.005235820057301055 1106 90-91 -0.0596167679414954 1106 92-93 0.03446141020994631 1106 94-95 0.014956101029067526 1106 96-97 -0.039327008418535314 1106 98-99 -0.0918918641399955 1106 100-101 -0.22108729966369012 1107 1 0.9051268306992668 1107 2 0.3871800726257071 1107 3 0.48841043703372833 1107 4 0.6489918101523031 1107 5 0.4797797257460559 1107 6 0.005849166659650962 1107 7 0.12720744067661371 1107 8 0.12884940714523907 1107 9 0.09818667348766752 1107 10-11 0.12290908955851165 1107 12-13 0.032610409037921784 1107 14-15 0.03011779262829606 1107 16-17 0.06193163917242828 1107 18-19 -0.06693905091556474 1107 20-21 -0.08304028811704711 1107 22-23 -0.11794283385520998 1107 24-25 -0.1021912626577901 1107 26-27 -0.1322903530113635 1107 28-29 -0.15767379862622022 1107 30-31 -0.21119000624595685 1107 32-33 -0.24346538856004685 1107 34-35 -0.20176770852058468 1107 36-37 -0.18312940041117542 1107 38-39 -0.24154299097062193 1107 40-41 -0.1557191441115151 1107 42-43 -0.14287625258040038 1107 44-45 -0.2006746100480825 1107 46-47 -0.27443745344250203 1107 48-49 -0.29786630008520376 1107 50-51 -0.2623818014257111 1107 52-53 -0.09440133604087464 1107 54-55 -0.074502347852345 1107 56-57 -0.0929833884336162 1107 58-59 -0.05410169491648986 1107 60-61 -0.08465999980867878 1107 62-63 -0.060776913282239775 1107 64-65 -0.0528647828820894 1107 66-67 -0.09735975482953663 1107 68-69 -0.21025138708682434 1107 70-71 -0.13524398153728967 1107 72-73 -0.17299162087071096 1107 74-75 -0.12688099936042008 1107 76-77 -0.2543921333957826 1107 78-79 -0.08983828583056308 1107 80-81 -0.05617757919700139 1107 82-83 -0.049382428981981974 1107 84-85 -0.05793065042069756 1107 86-87 -0.048805554097679504 1107 88-89 -0.0432063061087149 1107 90-91 -0.07054038719695654 1107 92-93 -0.13314996892492914 1107 94-95 -0.11615784586991396 1107 96-97 -0.05525279032620389 1107 98-99 -0.0637491609304206 1107 100-101 -0.12249132682455155 1108 1 0.8876455829858045 1108 2 0.37392406210889817 1108 3 0.5405078418772788 1108 4 0.6518919767727525 1108 5 0.4713925431551971 1108 6 0.26107332383952553 1108 7 0.2538813409159246 1108 8 0.16229099136312186 1108 9 0.14975821040464865 1108 10-11 0.19748910301600375 1108 12-13 0.15829065982752866 1108 14-15 -0.013176597716473282 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0.28151988780962967 2312 78-79 0.2289776365373477 2312 80-81 0.2324105565246697 2312 82-83 0.19653667208565295 2312 84-85 0.1961053782959965 2312 86-87 0.22670860577166962 2312 88-89 0.22564894932914825 2312 90-91 0.22942890067067978 2312 92-93 0.21818813400996717 2312 94-95 0.1650158636818304 2312 96-97 0.1524533961673633 2312 98-99 0.07845615094839431 2312 100-101 0.04828417389456163 2313 1 -0.658323395433051 2313 2 -0.1649342207832305 2313 3 0.26071245298289014 2313 4 0.4279811812410017 2313 5 0.28670444337085854 2313 6 0.04882254050872348 2313 7 -0.13582494810570012 2313 8 -0.11433681317237898 2313 9 -0.12291763340930828 2313 10-11 -0.3609361987773312 2313 12-13 -0.5154757719392578 2313 14-15 -0.24816189993829596 2313 16-17 -0.16729062391367933 2313 18-19 -0.06973363601050409 2313 20-21 -0.08632858635617424 2313 22-23 -0.0877117307269657 2313 24-25 -0.285525871145758 2313 26-27 -0.2194710287863586 2313 28-29 -0.07860700699470158 2313 30-31 -0.08439129558349379 2313 32-33 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50-51 -0.36473764625233684 2314 52-53 -0.3036502988326788 2314 54-55 -0.36813808089348754 2314 56-57 -0.3140908389210608 2314 58-59 -0.34785908455643977 2314 60-61 -0.34599819529298514 2314 62-63 -0.32390327610927017 2314 64-65 -0.2843236991585627 2314 66-67 -0.2841258862986429 2314 68-69 -0.1262541208166148 2314 70-71 -0.1503626836796279 2314 72-73 -0.18255936366686853 2314 74-75 -0.2547059354523995 2314 76-77 -0.24805019844920295 2314 78-79 -0.2893893274956092 2314 80-81 -0.2528798955185856 2314 82-83 -0.2480429677708642 2314 84-85 -0.26263686389293284 2314 86-87 -0.26663196613418805 2314 88-89 -0.25059596872982937 2314 90-91 -0.20842918181196524 2314 92-93 -0.24440450992185347 2314 94-95 -0.26232030998469824 2314 96-97 -0.29330733533212694 2314 98-99 -0.3414944728660352 2314 100-101 -0.34492167948682706 2315 1 -5.21931486088754 2315 2 -2.5538119014624776 2315 3 -3.6135852182437063 2315 4 -1.5987070284542 2315 5 -1.1462157595749716 2315 6 -1.086803094821967 2315 7 -1.0416488208676498 2315 8 -1.0226988003528632 2315 9 -1.0908506553926003 2315 10-11 -1.118188062571413 2315 12-13 -1.151472624431598 2315 14-15 -1.2641001208833558 2315 16-17 -1.3106000253371803 2315 18-19 -1.3410901720441117 2315 20-21 -1.481361254594205 2315 22-23 -1.3236984259854978 2315 24-25 -1.2120041236021635 2315 26-27 -1.163522503011606 2315 28-29 -1.1278994875602564 2315 30-31 -1.22966427033451 2315 32-33 -1.2689631662579686 2315 34-35 -1.2554374838431173 2315 36-37 -1.161645766952354 2315 38-39 -1.1322656334233372 2315 40-41 -1.1065859904001059 2315 42-43 -1.0989791894825132 2315 44-45 -1.1241574068966074 2315 46-47 -1.1157155539546224 2315 48-49 -1.0814287513431964 2315 50-51 -1.1098073097859356 2315 52-53 -1.005254129759983 2315 54-55 -1.0903547984234834 2315 56-57 -1.1535883273280412 2315 58-59 -1.188293168361099 2315 60-61 -1.260426545629059 2315 62-63 -1.2790655557601482 2315 64-65 -1.1605580186998452 2315 66-67 -1.161199294031988 2315 68-69 -1.1139715053680384 2315 70-71 -1.1514491317614635 2315 72-73 -1.175735249078901 2315 74-75 -1.3451585934856922 2315 76-77 -1.3133608935957923 2315 78-79 -1.3716584578473174 2315 80-81 -1.3628490158618476 2315 82-83 -1.3432664601754745 2315 84-85 -1.315126188012254 2315 86-87 -1.435458918285935 2315 88-89 -1.550358865104002 2315 90-91 -1.6137612807015351 2315 92-93 -1.5590234488029573 2315 94-95 -1.6512243598582863 2315 96-97 -1.703317809079465 2315 98-99 -1.5612050588432567 2315 100-101 -1.6811774741399788 2316 1 -9.077208132764483 2316 2 -5.767594691696271 2316 3 -8.685731763807855 2316 4 -10.706395284653738 2316 5 -7.044020741504074 2316 6 -5.5002737749290915 2316 7 -4.496101021677518 2316 8 -4.235290102334197 2316 9 -4.545214158321315 2316 10-11 -4.517862968297742 2316 12-13 -4.557333104240431 2316 14-15 -4.98507447533472 2316 16-17 -5.02536194221323 2316 18-19 -5.070613123755564 2316 20-21 -4.873463542356326 2316 22-23 -4.931270392236485 2316 24-25 -4.817463003262251 2316 26-27 -4.805108928870322 2316 28-29 -4.708618910243686 2316 30-31 -4.656528820633248 2316 32-33 -4.644460454321909 2316 34-35 -4.626332955816984 2316 36-37 -4.634422781195202 2316 38-39 -4.696947037495097 2316 40-41 -4.629219189418734 2316 42-43 -4.6060004088389235 2316 44-45 -4.658622835862655 2316 46-47 -4.634869529066371 2316 48-49 -4.6121248544468685 2316 50-51 -4.631004909738817 2316 52-53 -4.992859852004123 2316 54-55 -4.6306258997903775 2316 56-57 -4.5733427649847584 2316 58-59 -4.596043437687602 2316 60-61 -4.632521462769322 2316 62-63 -4.7042281100621 2316 64-65 -4.6712832841151055 2316 66-67 -4.6887399395050195 2316 68-69 -4.568054662789923 2316 70-71 -4.507774998107227 2316 72-73 -4.444431993034929 2316 74-75 -4.489419683712178 2316 76-77 -4.359631635716557 2316 78-79 -4.472297508990874 2316 80-81 -4.569390941350935 2316 82-83 -4.518649092296467 2316 84-85 -4.48691552151757 2316 86-87 -4.5252548253350575 2316 88-89 -4.5433096141057305 2316 90-91 -4.4888795118402065 2316 92-93 -4.544081673572229 2316 94-95 -4.593772861797614 2316 96-97 -4.630949090359916 2316 98-99 -4.785454623057486 2316 100-101 -4.951084009774352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1649536.0 3 505914.0 4 280680.0 5 270417.0 6 612578.0 7 565762.0 8 471002.0 9 744565.0 10 1027011.0 11 817816.0 12 525828.0 13 389517.0 14 335130.0 15 306920.0 16 286046.0 17 269206.0 18 257529.0 19 254083.0 20 257609.0 21 274304.0 22 304888.0 23 349561.0 24 401469.0 25 460963.0 26 538248.0 27 639420.0 28 688903.0 29 792641.0 30 941687.0 31 1143870.0 32 1403847.0 33 1785106.0 34 2381485.0 35 3398913.0 36 5365467.0 37 1.0347172E7 38 1.7259814E7 39 5813875.0 40 19080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.599911691823184 19.062881740763235 10.540446822212315 44.796759745201264 2 12.613347689580795 17.08627334147351 59.13450002324049 11.165878945705202 3 15.801769541152341 24.25488515165246 39.26202331550716 20.681321991688044 4 18.402086821245568 30.746577112904717 33.523162767463475 17.328173298386236 5 18.40278229479735 33.04540667698216 33.96609719307542 14.585713835145064 6 14.463312603689351 36.24348062089218 34.799145831539704 14.494060943878765 7 12.416094591912788 19.714304522394023 52.541145704977886 15.328455180715302 8 12.596906769839746 21.440196906569867 46.07263905934387 19.890257264246515 9 14.152224350463142 23.01814918808254 43.322658136884144 19.506968324570177 10-11 19.2270981317301 32.071093124024046 32.804338359635295 15.897470384610566 12-13 17.713866973393515 25.12604440800303 39.317441070031464 17.842647548572 14-15 17.98697374289623 27.39034035758542 38.025354272903314 16.597331626615038 16-17 18.909497282148386 26.878879752860275 36.96377131760091 17.24785164739043 18-19 18.69691106050759 28.12545293995312 36.02388627888483 17.153749720654464 20-21 19.63640494396511 27.361628285283196 35.14715030832761 17.854816462424086 22-23 19.608635383214754 29.429688416581396 33.82010898443987 17.141567215763974 24-25 18.391859271476005 29.68970990916618 33.39890190903251 18.519528910325313 26-27 18.560154833365683 29.567926763301582 33.40882359736072 18.46309480597201 28-29 18.46382709265029 30.69292386601483 32.24441018502183 18.598838856313048 30-31 18.353260750264088 30.840272052362174 32.20268522625866 18.603781971115076 32-33 18.4436632819611 30.272191945295525 32.11642558991844 19.16771918282494 34-35 19.65519466345601 30.57356930450351 30.74203931452675 19.029196717513727 36-37 19.112962047142478 30.89212800957128 31.044540458797748 18.9503694844885 38-39 19.26044339349548 30.41888392923337 32.041555072332024 18.27911760493913 40-41 19.49699165963135 30.166013357606996 31.92592611135553 18.411068871406123 42-43 19.535675408018687 29.807749348300224 32.02861994210701 18.62795530157408 44-45 19.613894102435925 29.560900505599662 31.75319239315319 19.07201299881122 46-47 19.34830857514898 29.598292661386367 32.36488542685531 18.688513336609347 48-49 19.040111573079006 30.061429925791654 31.682353045092082 19.216105456037255 50-51 18.400859179130816 30.0012488887413 32.50322088523722 19.094671046890667 52-53 20.038618687618595 29.851626444883983 31.007190217348025 19.102564650149397 54-55 19.00056818375631 30.54615224026224 30.501972166569217 19.951307409412227 56-57 19.91190393294898 30.191818914439633 30.826935607972345 19.06934154463904 58-59 19.605845249245352 30.54250040914172 30.752974271381532 19.098680070231396 60-61 19.76280643371116 31.007706443382947 29.68474743329831 19.544739689607585 62-63 19.90165564314186 30.53388921993773 31.01850320103904 18.545951935881373 64-65 20.420357430640507 30.1174493489916 30.37356847493435 19.088624745433545 66-67 20.187141449137886 31.927866112090197 28.756146513703705 19.128845925068212 68-69 20.00043094482179 31.118751776541853 30.21461373024797 18.666203548388392 70-71 19.91549798904408 31.044782377990078 29.603870235563107 19.43584939740274 72-73 19.287037722570442 31.90861037882498 29.35717227334872 19.447179625255863 74-75 19.28870449173683 30.46483366435923 31.031623305782936 19.214838538121004 76-77 19.21228457643898 31.154646174748457 30.243165287288964 19.389903961523597 78-79 19.396963788667083 31.679102892882284 29.765254171714457 19.158679146736176 80-81 19.61745889247323 30.777310477820187 30.23025821831699 19.374972411389596 82-83 20.120736364000013 31.314931680291586 29.266421588924267 19.297910366784134 84-85 19.500100404886183 30.827074634725026 29.997091061730185 19.675733898658603 86-87 19.84556550051341 31.33024334706104 29.630352241033464 19.193838911392092 88-89 19.686049422986716 30.71127540792392 30.340100468382413 19.262574700706953 90-91 19.6121401427129 31.396092712126293 30.132341483914328 18.859425661246483 92-93 20.035337995644024 30.764395449539407 29.965987730766535 19.234278824050037 94-95 19.49968553398805 30.90224433440746 30.23498664347853 19.36308348812596 96-97 19.377034477037657 31.218261534498552 29.73088379023501 19.67382019822878 98-99 19.865953349630892 30.71829124595838 30.113140498748315 19.30261490566241 100-101 20.21613421629212 31.740273275385483 28.821035227389853 19.222557280932545 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21087.0 1 28886.0 2 30530.5 3 27838.5 4 35805.5 5 46342.5 6 57065.5 7 68519.5 8 81797.5 9 95295.0 10 108528.5 11 122455.0 12 138776.5 13 154797.5 14 171636.5 15 189562.0 16 205603.5 17 223307.5 18 249445.0 19 276975.5 20 319745.0 21 374019.5 22 421554.0 23 489618.5 24 623967.5 25 756340.0 26 856072.0 27 1031680.5 28 1314882.5 29 1567207.5 30 1811801.5 31 1999046.5 32 2399609.5 33 2740864.5 34 2646225.0 35 2758488.0 36 2931984.0 37 3196188.0 38 3395926.0 39 3096993.0 40 2724528.5 41 2449929.0 42 2162051.0 43 1935814.5 44 1780587.0 45 1671479.0 46 1596481.5 47 1509666.0 48 1423617.5 49 1395703.5 50 1243578.5 51 994070.5 52 895094.0 53 832131.0 54 741381.0 55 655945.5 56 583372.5 57 492776.0 58 406031.5 59 336120.5 60 261783.0 61 193742.5 62 153319.0 63 118984.5 64 80988.0 65 59232.5 66 46835.5 67 38794.5 68 31138.0 69 23206.5 70 21061.5 71 26297.5 72 26313.5 73 37888.5 74 40827.0 75 22731.0 76 14447.0 77 17118.0 78 15838.0 79 7757.5 80 4831.5 81 3062.0 82 2094.0 83 1303.5 84 727.5 85 416.5 86 298.0 87 209.0 88 121.0 89 67.5 90 48.0 91 34.5 92 21.5 93 12.0 94 8.5 95 8.5 96 5.5 97 3.5 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06881582675768019 2 0.00640963055488192 3 0.005803124525728656 4 0.009752429851808906 5 0.04440434886962712 6 0.1791359992635863 7 0.1916465503636526 8 0.20180591613733553 9 0.21142737810624246 10-11 0.22652454489362306 12-13 0.24469088165115327 14-15 0.24719798112384847 16-17 0.24865110096747534 18-19 0.26023630160918054 20-21 0.2580145250242361 22-23 0.23002715619051972 24-25 0.24678246992392733 26-27 0.25336672432267854 28-29 0.2605949041456979 30-31 0.27816642843504824 32-33 0.29424897262711996 34-35 0.28834216519409395 36-37 0.28038352759560337 38-39 0.3155982966816075 40-41 0.3464755342172148 42-43 0.3364471363264338 44-45 0.37662231397735085 46-47 0.3600275918146445 48-49 0.3563238512690055 50-51 0.34463184943707664 52-53 0.3443816072322461 54-55 0.35698102939571014 56-57 0.3485374988021896 58-59 0.35811764352232384 60-61 0.3752572856263902 62-63 0.3793328814109831 64-65 0.27353343957739035 66-67 0.15898253671131102 68-69 0.39753975584655443 70-71 0.05439922521895101 72-73 0.023456503741892735 74-75 0.02964239749681709 76-77 0.0331598206376134 78-79 0.03610192057851882 80-81 0.05860422974498277 82-83 0.06253092751984779 84-85 0.03113059802336411 86-87 0.008971299978786321 88-89 0.013303374534062268 90-91 0.0038269127212254127 92-93 0.005007182808806443 94-95 0.013276089558457688 96-97 0.009202052915327923 98-99 6.470437071943557E-5 100-101 0.004159009852869745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.4137862E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.598077980764312 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04918952734546 15.688102586538985 2 7.758424868933761 3.0410043117853003 3 3.0604035254268536 1.7993408083176448 4 1.7202413196012418 1.3485369410911219 5 1.1098721999847199 1.0875680961991494 6 0.7774919196335636 0.914240836223563 7 0.6032808516095541 0.8276201621902162 8 0.46151129623224535 0.7235787498050531 9 0.36158045903886793 0.6377653829307861 >10 3.0971693728963747 12.57867545387966 >50 0.4831309926324961 6.669717919887974 >100 0.4279288575024405 16.954455652641006 >500 0.049772899142648555 6.726705400683686 >1k 0.03387911292412445 12.862660250061941 >5k 0.003522254780542656 4.7263152634079475 >10k+ 0.002600542315164633 13.413712184355841 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG 219542 0.3422970350960561 TruSeq Adapter, Index 6 (100% over 49bp) CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 183565 0.28620380267742634 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 171999 0.2681707725149928 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 140887 0.21966276331443663 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 129938 0.2025917234347475 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 110802 0.17275599239650363 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 108323 0.16889088070943184 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94824 0.14784403009878938 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 94671 0.14760548145493219 TruSeq Adapter, Index 6 (100% over 50bp) CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 91620 0.14284854085095633 No Hit AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT 87558 0.13651530822776722 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 75086 0.11706969589974794 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 73227 0.11417125191980985 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 66829 0.10419586483877495 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 66783 0.10412414433147149 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 66030 0.10295011080974292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.4106965710830835 0.0 0.0 1.5591414631189298E-6 0.0 2 0.41183786263408656 0.0 0.0 1.5591414631189298E-6 0.0 3 0.41207485213648065 0.0 0.0 1.5591414631189298E-6 0.0 4 0.41581523250650293 0.0 0.0 1.5591414631189298E-6 0.0 5 0.4163297491893322 0.0 0.0 1.5591414631189298E-6 0.0 6 0.41708437365748174 0.0 0.0 3.1182829262378595E-6 0.0 7 0.4176176000378684 0.0 0.0 3.1182829262378595E-6 0.0 8 0.4216588947102727 0.0 0.0 3.1182829262378595E-6 0.0 9 0.42209077689555663 0.0 0.0 6.236565852475719E-6 0.0 10-11 0.4242805910805072 0.0 0.0 7.795707315594649E-6 0.0 12-13 0.4269685509629242 0.0 0.0 9.354848778713577E-6 0.0 14-15 0.42922073080639955 7.795707315594649E-7 0.0 9.354848778713577E-6 0.0 16-17 0.43185412073760737 1.5591414631189298E-6 0.0 1.0134419510273042E-5 0.0 18-19 0.43417802108838616 1.5591414631189298E-6 0.0 1.5591414631189297E-5 0.0 20-21 0.43654947525379 2.3387121946783947E-6 0.0 1.8709697557427155E-5 0.0 22-23 0.43881334865823873 3.1182829262378595E-6 0.0 1.8709697557427155E-5 0.0 24-25 0.4406624904334978 3.1182829262378595E-6 0.0 2.104840975210555E-5 0.0 26-27 0.44239547616975444 3.1182829262378595E-6 0.0 2.9623687799259662E-5 0.0 28-29 0.44401074672554564 3.1182829262378595E-6 0.0 3.430111218861645E-5 0.0 30-31 0.4458053185495956 3.897853657797324E-6 0.0 3.5860253651735384E-5 0.0 32-33 0.44758585810047735 4.677424389356789E-6 0.0 3.7419395114854316E-5 7.795707315594649E-7 34-35 0.4508156196413282 6.236565852475719E-6 0.0 3.897853657797324E-5 1.5591414631189298E-6 36-37 0.452759089475106 6.236565852475719E-6 1.5591414631189298E-6 4.599467316200843E-5 1.5591414631189298E-6 38-39 0.45477817766984496 6.236565852475719E-6 1.5591414631189298E-6 4.677424389356789E-5 1.5591414631189298E-6 40-41 0.45679570672312086 6.236565852475719E-6 1.5591414631189298E-6 4.677424389356789E-5 1.5591414631189298E-6 42-43 0.45904320914220687 1.0913990241832508E-5 1.5591414631189298E-6 4.833338535668682E-5 1.5591414631189298E-6 44-45 0.46142713643931565 1.1693560973391974E-5 1.5591414631189298E-6 5.145166828292468E-5 1.5591414631189298E-6 46-47 0.46400673599004594 1.2473131704951438E-5 1.5591414631189298E-6 5.5349521940722E-5 1.5591414631189298E-6 48-49 0.4668217659017072 1.3252702436510902E-5 1.5591414631189298E-6 5.612909267228147E-5 1.5591414631189298E-6 50-51 0.46993459183282416 1.4032273168070367E-5 1.5591414631189298E-6 5.690866340384093E-5 1.5591414631189298E-6 52-53 0.4734278482809421 1.4032273168070367E-5 1.5591414631189298E-6 5.76882341354004E-5 1.5591414631189298E-6 54-55 0.47726099756802 1.5591414631189297E-5 1.5591414631189298E-6 5.76882341354004E-5 1.5591414631189298E-6 56-57 0.4815299268940396 1.5591414631189297E-5 1.5591414631189298E-6 6.158608779319772E-5 1.5591414631189298E-6 58-59 0.4860982113809781 1.5591414631189297E-5 1.5591414631189298E-6 6.314522925631665E-5 1.5591414631189298E-6 60-61 0.49134004497998385 1.5591414631189297E-5 1.5591414631189298E-6 6.392479998787612E-5 1.5591414631189298E-6 62-63 0.4974129009788322 1.5591414631189297E-5 1.5591414631189298E-6 6.392479998787612E-5 1.5591414631189298E-6 64-65 0.5041070748507332 1.5591414631189297E-5 1.5591414631189298E-6 6.62635121825545E-5 1.5591414631189298E-6 66-67 0.5118630240590183 1.7930126825867692E-5 2.3387121946783947E-6 7.016136584035184E-5 1.5591414631189298E-6 68-69 0.520759485247575 1.8709697557427155E-5 3.1182829262378595E-6 7.172050730347077E-5 2.3387121946783947E-6 70-71 0.5309664048358831 2.0268839020546087E-5 3.1182829262378595E-6 7.172050730347077E-5 3.1182829262378595E-6 72-73 0.5419700457118449 2.0268839020546087E-5 3.1182829262378595E-6 7.172050730347077E-5 3.1182829262378595E-6 74-75 0.5552726406751756 2.0268839020546087E-5 4.677424389356789E-6 7.717750242438702E-5 3.1182829262378595E-6 76-77 0.5701647803601562 2.1827980483665015E-5 4.677424389356789E-6 9.432805851869524E-5 3.1182829262378595E-6 78-79 0.5869614113423363 2.260755121522448E-5 4.677424389356789E-6 9.510762925025471E-5 3.1182829262378595E-6 80-81 0.6071328040214374 2.3387121946783944E-5 6.236565852475719E-6 9.978505363961149E-5 3.1182829262378595E-6 82-83 0.6302158933829132 2.3387121946783944E-5 6.236565852475719E-6 1.0602161949208722E-4 3.1182829262378595E-6 84-85 0.6582126170654083 2.3387121946783944E-5 6.236565852475719E-6 1.0758076095520615E-4 3.1182829262378595E-6 86-87 0.6904470872446606 2.3387121946783944E-5 6.236565852475719E-6 1.0836033168676562E-4 3.1182829262378595E-6 88-89 0.7286055154130333 2.3387121946783944E-5 7.795707315594649E-6 1.10699043881444E-4 3.1182829262378595E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 37165 0.0 52.615498 1 GTTTTTT 863290 0.0 47.547157 1 GCGTGTT 44850 0.0 44.211277 2 TATGCCG 51710 0.0 37.13162 46-47 AGATCGG 69385 0.0 37.06374 1 TGCCGTC 50520 0.0 36.830006 48-49 ACGCCAA 55635 0.0 35.70908 32-33 CCGTCTT 53645 0.0 34.886932 50-51 TCACGCC 56560 0.0 34.407253 30-31 CTCGTAT 55450 0.0 34.18389 42-43 ATCAATA 58535 0.0 33.813507 4 ATCGGAA 78370 0.0 33.42391 3 CGGAAGA 80510 0.0 33.34228 5 GATCGGA 79115 0.0 33.14966 2 AGTCACG 59450 0.0 32.3973 28-29 CGTCTGA 62230 0.0 31.959606 16-17 CATCAAT 61485 0.0 31.903841 3 ATCTCGT 58285 0.0 31.884897 40-41 TCGTACC 14555 0.0 31.368221 2 TCGGAAG 85980 0.0 30.704594 4 >>END_MODULE