FastQCFastQC Report
Fri 27 May 2016
SRR1233082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57259584
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2196250.38356024381874654Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1102010.1924586109462479No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT945360.1651007454053456No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT819020.14303631685483428No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG818080.14287215219726362No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT790240.1380100840411275No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT756250.13207395988067255No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA714790.12483325062228885No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT657020.11474410991180098No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT594100.10375555644972902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT438650.066.946751
GCGTGTT596700.048.9364972
GTTTTTT9604550.048.3040921
AGATCGG649550.043.087621
CGCCGTA448950.039.73108746-47
GTCGCCG447100.038.87496644-45
AGAGCGT702700.038.8488739
AAGAGCG718650.038.8059438
CCGTATC466400.038.717848-49
GATCGGA733350.038.087192
ATCGGAA750050.037.4104463
TCGGAAG750600.037.2931334
CGGAAGA773900.036.2120555
TCGGTGG497500.036.06947338-39
TGGTCGC472250.034.88143542-43
TCTCGGT510700.034.3569136-37
CGTGTTT917550.032.539393
GATCTCG585900.032.2042134-35
AGCGTCG584700.030.828610-11
GGTGGTC539650.030.75656940-41