Basic Statistics
Measure | Value |
---|---|
Filename | SRR1233082_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 57259584 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 219625 | 0.38356024381874654 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 110201 | 0.1924586109462479 | No Hit |
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT | 94536 | 0.1651007454053456 | No Hit |
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT | 81902 | 0.14303631685483428 | No Hit |
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG | 81808 | 0.14287215219726362 | No Hit |
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT | 79024 | 0.1380100840411275 | No Hit |
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT | 75625 | 0.13207395988067255 | No Hit |
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA | 71479 | 0.12483325062228885 | No Hit |
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT | 65702 | 0.11474410991180098 | No Hit |
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT | 59410 | 0.10375555644972902 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCGTGT | 43865 | 0.0 | 66.94675 | 1 |
GCGTGTT | 59670 | 0.0 | 48.936497 | 2 |
GTTTTTT | 960455 | 0.0 | 48.304092 | 1 |
AGATCGG | 64955 | 0.0 | 43.08762 | 1 |
CGCCGTA | 44895 | 0.0 | 39.731087 | 46-47 |
GTCGCCG | 44710 | 0.0 | 38.874966 | 44-45 |
AGAGCGT | 70270 | 0.0 | 38.848873 | 9 |
AAGAGCG | 71865 | 0.0 | 38.805943 | 8 |
CCGTATC | 46640 | 0.0 | 38.7178 | 48-49 |
GATCGGA | 73335 | 0.0 | 38.08719 | 2 |
ATCGGAA | 75005 | 0.0 | 37.410446 | 3 |
TCGGAAG | 75060 | 0.0 | 37.293133 | 4 |
CGGAAGA | 77390 | 0.0 | 36.212055 | 5 |
TCGGTGG | 49750 | 0.0 | 36.069473 | 38-39 |
TGGTCGC | 47225 | 0.0 | 34.881435 | 42-43 |
TCTCGGT | 51070 | 0.0 | 34.35691 | 36-37 |
CGTGTTT | 91755 | 0.0 | 32.53939 | 3 |
GATCTCG | 58590 | 0.0 | 32.20421 | 34-35 |
AGCGTCG | 58470 | 0.0 | 30.8286 | 10-11 |
GGTGGTC | 53965 | 0.0 | 30.756569 | 40-41 |