##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233082_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57259584 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0305937430492 33.0 31.0 34.0 27.0 34.0 2 31.34727026658105 33.0 31.0 34.0 28.0 34.0 3 31.285240511003362 34.0 31.0 34.0 27.0 34.0 4 34.61986126200288 37.0 35.0 37.0 31.0 37.0 5 34.576932727977905 37.0 35.0 37.0 30.0 37.0 6 34.62965331707614 37.0 35.0 37.0 31.0 37.0 7 34.749456405411536 37.0 35.0 37.0 32.0 37.0 8 34.7297484557345 37.0 35.0 37.0 32.0 37.0 9 36.27934006645944 39.0 37.0 39.0 32.0 39.0 10-11 36.176626082369026 39.0 37.0 39.0 31.5 39.0 12-13 36.10401038715196 39.0 37.0 39.0 30.5 39.0 14-15 37.311334692896125 40.0 38.0 41.0 31.0 41.0 16-17 37.04485033457456 40.0 37.0 41.0 31.0 41.0 18-19 36.56986677549037 40.0 36.0 41.0 29.5 41.0 20-21 36.168850152666145 40.0 35.0 41.0 28.0 41.0 22-23 35.634822591446 39.0 35.0 41.0 26.0 41.0 24-25 35.14671627722618 39.0 35.0 41.0 22.5 41.0 26-27 34.751185426006586 40.0 35.0 41.0 17.0 41.0 28-29 34.34185957411077 40.0 35.0 41.0 10.5 41.0 30-31 34.011508108406794 40.0 34.0 41.0 8.5 41.0 32-33 33.7867849511446 40.0 34.0 41.0 7.0 41.0 34-35 33.59666848959294 39.5 34.0 41.0 2.0 41.0 36-37 33.44402322238317 39.0 34.0 41.0 2.0 41.0 38-39 33.291533413864826 39.0 34.0 41.0 2.0 41.0 40-41 33.15443424283348 39.0 33.0 41.0 2.0 41.0 42-43 32.98262919618836 39.0 33.0 41.0 2.0 41.0 44-45 32.68447277751791 39.0 33.0 41.0 2.0 41.0 46-47 32.571127586257 39.0 33.0 41.0 2.0 41.0 48-49 32.58133974567471 39.0 33.0 41.0 2.0 41.0 50-51 31.932854620459693 38.0 31.5 40.0 2.0 40.5 52-53 31.925539967248103 38.0 31.5 40.0 2.0 40.5 54-55 32.208499410334525 39.0 32.0 41.0 2.0 41.0 56-57 32.114292351477786 39.0 32.0 41.0 2.0 41.0 58-59 31.95210645435356 38.5 31.0 41.0 2.0 41.0 60-61 31.84926856087533 38.0 31.0 41.0 2.0 41.0 62-63 31.63703263195206 38.0 31.0 40.5 2.0 41.0 64-65 31.329996564417932 38.0 31.0 40.0 2.0 41.0 66-67 30.969853876339723 37.0 30.5 40.0 2.0 41.0 68-69 30.654545770014675 37.0 30.0 40.0 2.0 41.0 70-71 30.316187924103673 36.0 29.0 39.0 2.0 41.0 72-73 29.96526126525823 36.0 29.0 39.0 2.0 41.0 74-75 29.58113677528639 35.0 29.0 39.0 2.0 40.5 76-77 29.199861948001576 35.0 28.0 38.0 2.0 40.0 78-79 28.803802896996245 35.0 28.0 37.0 2.0 39.0 80-81 28.412077801682948 35.0 27.0 37.0 2.0 39.0 82-83 28.040458816466426 35.0 26.0 36.0 2.0 39.0 84-85 27.67160664317785 34.5 26.0 36.0 2.0 37.5 86-87 27.31710168379847 34.0 26.0 35.5 2.0 37.0 88-89 26.9866773918581 34.0 25.0 35.0 2.0 37.0 90-91 26.687440376793518 34.0 25.0 35.0 2.0 36.0 92-93 26.360311707818205 34.0 23.5 35.0 2.0 36.0 94-95 26.042679580068203 34.0 21.0 35.0 2.0 36.0 96-97 25.678500991205247 34.0 18.5 35.0 2.0 35.5 98-99 25.237344258386507 34.0 2.0 35.0 2.0 35.0 100-101 23.12193058370805 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7095966063358006 1101 2 0.7160127254036048 1101 3 0.775674167078023 1101 4 0.8501840374722605 1101 5 0.8572554383396422 1101 6 0.7497950259153896 1101 7 0.7273453819156828 1101 8 0.6778678660056485 1101 9 0.8528944580792057 1101 10-11 0.8258637359175012 1101 12-13 0.7531084561413977 1101 14-15 0.9430281404660974 1101 16-17 1.0067037838409405 1101 18-19 0.9656846988770198 1101 20-21 1.0025551456046387 1101 22-23 1.031962275892937 1101 24-25 1.005969244433004 1101 26-27 0.8948484706221436 1101 28-29 0.9832574804644665 1101 30-31 1.0080023022875224 1101 32-33 0.8848612650155374 1101 34-35 0.8990826936559984 1101 36-37 0.9119077895241929 1101 38-39 0.9377120928400231 1101 40-41 0.9806615127432821 1101 42-43 1.005892342068286 1101 44-45 0.9842874498806324 1101 46-47 0.9454399673214695 1101 48-49 0.93659485366166 1101 50-51 0.9624463845338838 1101 52-53 1.0214706429728224 1101 54-55 1.0688215175462972 1101 56-57 1.031226395491025 1101 58-59 1.0919564316429344 1101 60-61 1.0909262590491373 1101 62-63 1.10827993737524 1101 64-65 1.0385283254729814 1101 66-67 1.1123844773857208 1101 68-69 1.0408971389526585 1101 70-71 0.9551849660423244 1101 72-73 0.871252099143387 1101 74-75 0.9718537620655283 1101 76-77 1.0393685646878552 1101 78-79 0.9587157327913545 1101 80-81 1.0011776919073583 1101 82-83 1.0437294607692635 1101 84-85 0.9979651525546771 1101 86-87 0.9927202463205127 1101 88-89 1.039264049613326 1101 90-91 0.9899732996743893 1101 92-93 0.8842496298739242 1101 94-95 1.0131116403328946 1101 96-97 1.059321018836215 1101 98-99 1.0948179570784617 1101 100-101 1.293215470439197 1102 1 0.6561020539614049 1102 2 0.6902946626978768 1102 3 0.7421030694984196 1102 4 0.7339513851484867 1102 5 0.7809520314615099 1102 6 0.7099536868517262 1102 7 0.661908646844374 1102 8 0.6408146078133825 1102 9 0.8083580745593224 1102 10-11 0.7917735933038799 1102 12-13 0.7993344311185595 1102 14-15 0.9477394799456036 1102 16-17 1.0059755460082371 1102 18-19 0.9354388963024078 1102 20-21 0.9400617253330878 1102 22-23 0.9089729603801686 1102 24-25 0.9444365035539448 1102 26-27 0.9910714317547118 1102 28-29 1.0070820309443604 1102 30-31 0.9974714537336169 1102 32-33 0.9234917148824806 1102 34-35 0.9589742854277432 1102 36-37 0.9218143295296741 1102 38-39 0.9502284512361072 1102 40-41 1.0257497703915845 1102 42-43 1.000014464716969 1102 44-45 1.0469169890465508 1102 46-47 1.0356107684960207 1102 48-49 1.0295457755407824 1102 50-51 1.0412342074513319 1102 52-53 1.04707641914014 1102 54-55 1.1727479673716346 1102 56-57 1.0953032817098887 1102 58-59 1.1605350119844786 1102 60-61 1.110928326666258 1102 62-63 1.0868801062886924 1102 64-65 1.0881676492010968 1102 66-67 1.1469187928279716 1102 68-69 1.100932677074276 1102 70-71 1.0321000621721375 1102 72-73 1.0567022134825557 1102 74-75 1.0334213030322275 1102 76-77 1.0368730471589345 1102 78-79 1.0444514006363903 1102 80-81 0.9511046791255922 1102 82-83 1.0363996004700233 1102 84-85 1.072872500278045 1102 86-87 0.9819658669131677 1102 88-89 1.042799127082258 1102 90-91 0.9602416753546272 1102 92-93 0.9831881731954688 1102 94-95 1.0513481002488625 1102 96-97 1.0649853821075865 1102 98-99 1.0625667027985237 1102 100-101 1.0631770724097507 1103 1 0.5593474110348247 1103 2 0.6414432927856275 1103 3 0.5906754886744423 1103 4 0.6724974828531955 1103 5 0.7188918607000119 1103 6 0.6658942528523895 1103 7 0.6290092764482722 1103 8 0.6338480102536295 1103 9 0.69759614380785 1103 10-11 0.72780202087921 1103 12-13 0.7854703127339278 1103 14-15 0.9045595067421104 1103 16-17 0.9277011509321653 1103 18-19 0.8756310019374212 1103 20-21 0.8447194915795748 1103 22-23 0.9159441393137158 1103 24-25 0.9490426463492625 1103 26-27 0.9742372733213855 1103 28-29 0.8240931668214415 1103 30-31 0.881167524429948 1103 32-33 0.9139396078519226 1103 34-35 0.9260122546345144 1103 36-37 0.9555509354246325 1103 38-39 0.9780787100897683 1103 40-41 0.9470531827062345 1103 42-43 0.9006053472519326 1103 44-45 0.9793249874853771 1103 46-47 0.9408607995364875 1103 48-49 0.8908086635217245 1103 50-51 0.9255939103634248 1103 52-53 0.9161080253679827 1103 54-55 0.9814401100982764 1103 56-57 0.9279309325763663 1103 58-59 1.0787138851150146 1103 60-61 1.0305190989223014 1103 62-63 1.0114445181407952 1103 64-65 0.9817911555238368 1103 66-67 1.0090648477490483 1103 68-69 0.9963721035562969 1103 70-71 1.026611039149799 1103 72-73 0.9885534995538556 1103 74-75 0.9369134718105201 1103 76-77 0.9993192062081775 1103 78-79 0.975694845846391 1103 80-81 0.8945354538733312 1103 82-83 0.9752348679700411 1103 84-85 0.9043088219286339 1103 86-87 0.8827592956658314 1103 88-89 0.9456669222155369 1103 90-91 0.9446817605524842 1103 92-93 0.9579026506928052 1103 94-95 0.9496235932852635 1103 96-97 1.012748379975104 1103 98-99 1.0603368896201921 1103 100-101 1.1589042079591962 1104 1 0.4622328417147692 1104 2 0.5713659601313097 1104 3 0.6617041342487724 1104 4 0.6242959597692277 1104 5 0.6179794090728237 1104 6 0.5998307625387653 1104 7 0.5831590777408024 1104 8 0.522284269864997 1104 9 0.6171503326183725 1104 10-11 0.6033107580741515 1104 12-13 0.6552698156592172 1104 14-15 0.7862212085103053 1104 16-17 0.7429978297832847 1104 18-19 0.7461186773587158 1104 20-21 0.8017467938492118 1104 22-23 0.8200049018095683 1104 24-25 0.7753674304511335 1104 26-27 0.7889286770735637 1104 28-29 0.6867327816192415 1104 30-31 0.7053256005915998 1104 32-33 0.7723192134350185 1104 34-35 0.7953682871082322 1104 36-37 0.7620633064011031 1104 38-39 0.7381219523805314 1104 40-41 0.7314507473147458 1104 42-43 0.7571082853697177 1104 44-45 0.7705016424003688 1104 46-47 0.8062820812691243 1104 48-49 0.823701508952901 1104 50-51 0.716915447349713 1104 52-53 0.7842027721096443 1104 54-55 0.9123549338908816 1104 56-57 0.8601075422587101 1104 58-59 0.9896115388972291 1104 60-61 0.9081445348058743 1104 62-63 0.8961806465667514 1104 64-65 0.7980168739899796 1104 66-67 0.9080514121802636 1104 68-69 0.9737415472462843 1104 70-71 0.9663893724130368 1104 72-73 0.8676690446451154 1104 74-75 0.909758678929105 1104 76-77 0.9031541523066267 1104 78-79 0.932335114229538 1104 80-81 0.9352445269044054 1104 82-83 0.9132745857167741 1104 84-85 0.9302599138977712 1104 86-87 0.9343822360194203 1104 88-89 0.9819049620371452 1104 90-91 0.9626694877333897 1104 92-93 0.8951593963269708 1104 94-95 0.9193729892270248 1104 96-97 0.9667840288069627 1104 98-99 1.0069312501213155 1104 100-101 1.0628270664455997 1105 1 0.4493999532911346 1105 2 0.47596660522596324 1105 3 0.42531128382027106 1105 4 0.514629625515937 1105 5 0.5045656428710359 1105 6 0.5520869659545724 1105 7 0.5254724797894497 1105 8 0.529554111726469 1105 9 0.5823241323627286 1105 10-11 0.5464674097170175 1105 12-13 0.4906765162285467 1105 14-15 0.5330318582665754 1105 16-17 0.5375537991941428 1105 18-19 0.4996727024163121 1105 20-21 0.5443493765115477 1105 22-23 0.5794056505537313 1105 24-25 0.6059279408070779 1105 26-27 0.6256538943485026 1105 28-29 0.6227893299010248 1105 30-31 0.559720562797132 1105 32-33 0.601068049341535 1105 34-35 0.6505227080758544 1105 36-37 0.5843345389790215 1105 38-39 0.6292103969687801 1105 40-41 0.6434764910796176 1105 42-43 0.6528827420593544 1105 44-45 0.6647658735209561 1105 46-47 0.6068854299981226 1105 48-49 0.6768252331568476 1105 50-51 0.6048835266922623 1105 52-53 0.6625711276808275 1105 54-55 0.7418447626013744 1105 56-57 0.6912070298768427 1105 58-59 0.8514220867537006 1105 60-61 0.8056573289801428 1105 62-63 0.7491204888774625 1105 64-65 0.7479835884407748 1105 66-67 0.7776417531628503 1105 68-69 0.7756000061641224 1105 70-71 0.7806683500783258 1105 72-73 0.7222143398152916 1105 74-75 0.7216377958431046 1105 76-77 0.7059788404045477 1105 78-79 0.7150341684538581 1105 80-81 0.6693658236470341 1105 82-83 0.6685872022640069 1105 84-85 0.6532252851550986 1105 86-87 0.7088901336261806 1105 88-89 0.7306542426540865 1105 90-91 0.7354012885229473 1105 92-93 0.6884291075615572 1105 94-95 0.721706286393605 1105 96-97 0.7722908117476095 1105 98-99 0.7243944680493009 1105 100-101 0.805316282542762 1106 1 0.3656222862308809 1106 2 0.4114742098136226 1106 3 0.452206205655024 1106 4 0.49517844861482274 1106 5 0.5079334645807236 1106 6 0.47189275353714777 1106 7 0.3651847878853687 1106 8 0.40053318616240574 1106 9 0.47314102803184 1106 10-11 0.43509414180501693 1106 12-13 0.4373783959766726 1106 14-15 0.4749242061683816 1106 16-17 0.46885595142045844 1106 18-19 0.507711317979755 1106 20-21 0.5090186209424985 1106 22-23 0.5010924012848932 1106 24-25 0.5419318841018921 1106 26-27 0.5256251120382487 1106 28-29 0.511079983775133 1106 30-31 0.5464505197536127 1106 32-33 0.5611829461094686 1106 34-35 0.526963272579664 1106 36-37 0.48450205104055755 1106 38-39 0.5273889095902078 1106 40-41 0.5316072589387275 1106 42-43 0.5003299940130219 1106 44-45 0.4956275294054038 1106 46-47 0.49835821660027335 1106 48-49 0.5226650995455131 1106 50-51 0.4478730882728712 1106 52-53 0.5428962080472033 1106 54-55 0.6793445864181393 1106 56-57 0.6028887836092665 1106 58-59 0.7504516159008645 1106 60-61 0.697687861139773 1106 62-63 0.6252001423615319 1106 64-65 0.5436482671529888 1106 66-67 0.5855129155950856 1106 68-69 0.6111321396992153 1106 70-71 0.6521695303264217 1106 72-73 0.596531330974468 1106 74-75 0.6303321717549508 1106 76-77 0.6407188979834402 1106 78-79 0.6609724837316975 1106 80-81 0.6955936458899643 1106 82-83 0.6673393623902761 1106 84-85 0.6332438254708457 1106 86-87 0.6488285223576185 1106 88-89 0.6756286039665049 1106 90-91 0.6708995742567687 1106 92-93 0.6653644968941101 1106 94-95 0.6879876242641778 1106 96-97 0.6147710135702127 1106 98-99 0.6640630410486388 1106 100-101 0.6667872216118838 1107 1 0.3386641291545196 1107 2 0.3810736895403295 1107 3 0.3932938923198783 1107 4 0.4425712396817616 1107 5 0.45790499541612917 1107 6 0.4457071837926918 1107 7 0.4212109715776009 1107 8 0.40843170119902084 1107 9 0.4423330160857404 1107 10-11 0.410652569902588 1107 12-13 0.41133140451403705 1107 14-15 0.46885575234517063 1107 16-17 0.4344451205081512 1107 18-19 0.41320490116874 1107 20-21 0.4657841514176937 1107 22-23 0.4729831325349778 1107 24-25 0.4439519016291982 1107 26-27 0.45738797340633397 1107 28-29 0.5384781653405497 1107 30-31 0.4769399146599014 1107 32-33 0.46614439227250415 1107 34-35 0.4748119897699894 1107 36-37 0.4183661006893331 1107 38-39 0.48635048920323953 1107 40-41 0.47657388760764974 1107 42-43 0.4661196706755888 1107 44-45 0.457326216047349 1107 46-47 0.5333636913901003 1107 48-49 0.4862070275365511 1107 50-51 0.43930246141703577 1107 52-53 0.44646852584417474 1107 54-55 0.5782652854737904 1107 56-57 0.5321133128477626 1107 58-59 0.5938335763473219 1107 60-61 0.5067236450418484 1107 62-63 0.5532644638268742 1107 64-65 0.4851705585549979 1107 66-67 0.5085987023177232 1107 68-69 0.5741063991928428 1107 70-71 0.5845048111918416 1107 72-73 0.5537060053332077 1107 74-75 0.640423862441672 1107 76-77 0.6011804399782754 1107 78-79 0.5617038466175188 1107 80-81 0.5049903520097274 1107 82-83 0.5560465077003443 1107 84-85 0.6076775665380829 1107 86-87 0.5425078027759263 1107 88-89 0.5327023418386077 1107 90-91 0.5766028254920847 1107 92-93 0.5565428809880935 1107 94-95 0.5961804048284982 1107 96-97 0.615050466063046 1107 98-99 0.5260622855663364 1107 100-101 0.4409089029847877 1108 1 0.18498654086943844 1108 2 0.29156036312609857 1108 3 0.34464926897777204 1108 4 0.3152523851739204 1108 5 0.2772007572673587 1108 6 0.29598628288155027 1108 7 0.25855589967039094 1108 8 0.25289863699080684 1108 9 0.2549606772910167 1108 10-11 0.2927658957349095 1108 12-13 0.24698492139965822 1108 14-15 0.25726837360598154 1108 16-17 0.2937184990931385 1108 18-19 0.29042211928662454 1108 20-21 0.32398145558677527 1108 22-23 0.2720910030043484 1108 24-25 0.2940612181402429 1108 26-27 0.2841889881834234 1108 28-29 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0.25797860316885135 2311 82-83 0.2917003632134545 2311 84-85 0.3158766251824794 2311 86-87 0.25611187354550324 2311 88-89 0.2623842056763408 2311 90-91 0.3167429613892452 2311 92-93 0.268164963723617 2311 94-95 0.28715181025931 2311 96-97 0.3853622923949658 2311 98-99 0.5245141513823022 2311 100-101 0.8436251117638847 2312 1 0.09300829625968632 2312 2 0.11355909403923548 2312 3 0.06560458800027291 2312 4 -0.023209518286435582 2312 5 0.029586525266097397 2312 6 -0.011296290725340441 2312 7 -0.0399828030061542 2312 8 -0.0018537692568898478 2312 9 0.009502672753939123 2312 10-11 0.0012615213778701673 2312 12-13 0.01703503469722989 2312 14-15 -0.013912895727536068 2312 16-17 -0.026045294006408426 2312 18-19 0.011971150188337276 2312 20-21 0.05517191998970361 2312 22-23 0.08434655040696981 2312 24-25 0.04453325207280301 2312 26-27 0.10580822784941546 2312 28-29 0.09306013054865048 2312 30-31 0.08083376334212033 2312 32-33 0.07565710609772935 2312 34-35 0.10143868836902925 2312 36-37 0.12209790786003794 2312 38-39 0.0472682056618865 2312 40-41 0.05988329876422682 2312 42-43 0.06809625063589664 2312 44-45 0.20658788886181156 2312 46-47 0.12900027916425216 2312 48-49 0.10698933360473006 2312 50-51 0.05143593636451271 2312 52-53 0.13172184147133947 2312 54-55 0.08550059791090803 2312 56-57 0.11961759728623633 2312 58-59 0.04381636764700403 2312 60-61 0.054843887944802105 2312 62-63 0.09891272806739337 2312 64-65 0.04321162192224648 2312 66-67 0.0510727673007203 2312 68-69 0.03316140201253859 2312 70-71 0.0924004074036695 2312 72-73 0.11132417800559224 2312 74-75 0.06429795700640994 2312 76-77 0.07536322410026841 2312 78-79 0.06207840818491661 2312 80-81 0.08487485609778389 2312 82-83 0.11765513453591936 2312 84-85 0.1290037924612939 2312 86-87 0.12225144446234637 2312 88-89 0.0747254426438353 2312 90-91 0.15634901580753535 2312 92-93 0.18304790731349385 2312 94-95 0.10176529124461098 2312 96-97 0.192905357840381 2312 98-99 0.2884907995727808 2312 100-101 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-1.7155584647412496 2315 34-35 -1.6210705841980655 2315 36-37 -1.4880144227486838 2315 38-39 -1.5410716310074513 2315 40-41 -1.3891072102463724 2315 42-43 -1.358435888663891 2315 44-45 -1.2274314720213262 2315 46-47 -1.398152127762419 2315 48-49 -1.2885558696859682 2315 50-51 -1.3219337751594153 2315 52-53 -1.2654455216502356 2315 54-55 -1.2845704971793523 2315 56-57 -1.238999721574416 2315 58-59 -1.3868980092482737 2315 60-61 -1.410927945483639 2315 62-63 -1.3467378152937322 2315 64-65 -1.3638241001407216 2315 66-67 -1.2928433468359763 2315 68-69 -1.2837471284284234 2315 70-71 -1.3212214977250447 2315 72-73 -1.3430231879543904 2315 74-75 -1.373479085447471 2315 76-77 -1.390476817648313 2315 78-79 -1.2996980146521189 2315 80-81 -1.4274149706339543 2315 82-83 -1.3553454704531056 2315 84-85 -1.3150978015676884 2315 86-87 -1.351250537402155 2315 88-89 -1.446634865103423 2315 90-91 -1.2913259985354166 2315 92-93 -1.2666545235194206 2315 94-95 -1.2269787468567266 2315 96-97 -1.2857847897008767 2315 98-99 -1.2156511466371143 2315 100-101 -1.03811524897867 2316 1 -2.653252567047911 2316 2 -2.4677315519124683 2316 3 -2.925040904900044 2316 4 -2.8804955901713782 2316 5 -2.8973277519085556 2316 6 -3.014258468747606 2316 7 -2.9786966151422583 2316 8 -2.990117736840194 2316 9 -3.4069090287353063 2316 10-11 -3.328078898983726 2316 12-13 -3.3461977166508987 2316 14-15 -3.6999941731613504 2316 16-17 -3.8083480301287267 2316 18-19 -3.713130367201508 2316 20-21 -3.8468282991943354 2316 22-23 -3.725243198507407 2316 24-25 -3.8266493714684096 2316 26-27 -3.8609881226640823 2316 28-29 -3.9139160202326835 2316 30-31 -3.8573288420936933 2316 32-33 -3.764150573084926 2316 34-35 -3.8406779590457347 2316 36-37 -3.797923464986887 2316 38-39 -3.846329836046049 2316 40-41 -3.8249597133934117 2316 42-43 -3.787804531233043 2316 44-45 -3.691830730009883 2316 46-47 -3.725547236880729 2316 48-49 -3.9256870128006724 2316 50-51 -4.019095925815542 2316 52-53 -3.962565156375831 2316 54-55 -4.090660367606567 2316 56-57 -4.0788204229558715 2316 58-59 -4.172535269071346 2316 60-61 -4.071227350665584 2316 62-63 -4.051082504568164 2316 64-65 -4.04391035105926 2316 66-67 -4.038608752005711 2316 68-69 -3.896806836203929 2316 70-71 -3.8849076860745804 2316 72-73 -3.8859338301883035 2316 74-75 -3.7888618098795845 2316 76-77 -3.811398206821174 2316 78-79 -3.737296057535133 2316 80-81 -3.717595941554123 2316 82-83 -3.6306632406739716 2316 84-85 -3.5632992535813734 2316 86-87 -3.523641802345626 2316 88-89 -3.531355789394489 2316 90-91 -3.5301402941791586 2316 92-93 -3.444781098472294 2316 94-95 -3.4172759050009027 2316 96-97 -3.339873764648061 2316 98-99 -3.339241619101692 2316 100-101 -3.041033782048924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 621740.0 3 171745.0 4 161116.0 5 184083.0 6 244935.0 7 384781.0 8 668934.0 9 1139639.0 10 1314004.0 11 987283.0 12 686381.0 13 536745.0 14 453765.0 15 384482.0 16 346495.0 17 331160.0 18 326132.0 19 334123.0 20 353715.0 21 380859.0 22 421726.0 23 470496.0 24 535539.0 25 549377.0 26 565768.0 27 634366.0 28 722990.0 29 831747.0 30 972119.0 31 1161387.0 32 1410722.0 33 1771130.0 34 2321901.0 35 3256387.0 36 5009290.0 37 8912195.0 38 1.2983255E7 39 4703220.0 40 13852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.995530705557083 17.628366669798933 9.078883382642763 44.297219242001226 2 13.04911346075612 15.856763872025729 59.865556160609344 11.228566506608805 3 16.939563714304377 22.082953935049815 40.09803669160143 20.879445659044375 4 19.00940751418355 28.23344367473442 36.098318294465095 16.658830516616934 5 19.12265483022297 30.26536484746305 36.40781182587544 14.204168496438538 6 15.266362565139454 34.3485400023617 36.702586924698444 13.682510507800405 7 12.377916157340293 18.57088609075277 54.59682773992503 14.454370011981899 8 12.928293203597113 20.728005348968665 48.88560355518159 17.45809789225263 9 14.179318818814716 21.39462111689681 46.01983534756298 18.406224716725493 10-11 18.54651472087093 30.657012996396322 35.246066957108454 15.550405325624297 12-13 16.934866409409768 24.14832475203355 42.182694760921784 16.7341140776349 14-15 17.142149589475945 25.768436610296824 41.0984494164811 15.990964383746128 16-17 18.611963176711875 25.774809741458952 39.214288706540316 16.39893837528886 18-19 18.457204677887567 27.227352237389642 38.4842987199344 15.831144364788388 20-21 18.640966564207403 27.667170133605467 36.922996133920805 16.768867168266322 22-23 19.4720875869559 27.976813880865077 35.74445972412257 16.806638808056455 24-25 18.50124088497344 28.93507339080391 35.25519897765879 17.308486746563858 26-27 18.20651957073419 29.230421913212613 34.81622637101462 17.746832145038574 28-29 18.50163501646161 30.02449629330276 33.48166444732607 17.992204242909565 30-31 18.561075653275164 29.57245584578041 33.65892539853612 18.207543102408305 32-33 18.893198431221773 30.20030872268241 32.49970318488778 18.406789661208038 34-35 19.41166398134403 30.670678997215045 32.062464679306466 17.855192342134462 36-37 19.034645970264947 30.092569220875454 32.78742557890413 18.085359229955472 38-39 19.166728280948256 29.67362566831681 32.798673474384785 18.36097257635015 40-41 19.520935079257036 29.06689450935428 33.267942633502614 18.144227777886073 42-43 19.893603594705834 29.218269475329222 33.14943533366488 17.73869159630006 44-45 20.199222859877125 29.078625761195344 32.92687281519072 17.795278563736815 46-47 18.958632267027486 29.259847310684844 33.0736201477042 18.707900274583473 48-49 18.97270066498045 29.83572728923423 32.254660630986784 18.936911414798537 50-51 18.675835762953522 29.658181286106334 32.89383100707447 18.77215194386568 52-53 18.534313160711218 30.499814959460704 32.38035004957812 18.58552183024996 54-55 18.686817079922974 30.509403493197112 31.517130849194825 19.286648577685096 56-57 18.885462116872286 30.694196219198673 31.64966560632438 18.77067605760466 58-59 18.999400703756148 30.664412531879332 31.42760629629187 18.908580468072646 60-61 19.166019537652552 31.790680879051493 30.145172014123133 18.898127569172825 62-63 19.19129757028848 31.49140014426538 30.596349595328277 18.720952690117866 64-65 19.74328608113252 30.780017473477834 30.762629484551983 18.714066960837663 66-67 19.483207922529175 32.04056879221502 29.640034697060045 18.83618858819576 68-69 19.670283161740613 31.08101913942279 30.873902224376014 18.37479547446058 70-71 19.89394041034935 30.987089859529615 30.37963240897356 18.73933732114747 72-73 19.083334659475536 31.99970323560627 30.0406691381294 18.876292966788792 74-75 19.075641957127136 30.72541805828252 31.140877989537774 19.058061995052572 76-77 19.380104542955923 31.21312446514865 30.466833145191075 18.939937846704353 78-79 19.23991532997184 31.330391130097606 30.192017667554737 19.23767587237581 80-81 19.26564319075145 30.745509935713766 30.591113430075694 19.397733443459096 82-83 19.592751131576357 30.804392708662853 30.43910784107302 19.163748318687766 84-85 19.3493090327215 30.92672101955395 30.395176600124017 19.32879334760053 86-87 19.749945575019836 30.85970206610522 30.148372973949737 19.241979384925205 88-89 19.581398494005718 31.037401230886303 30.112619858965108 19.268580416142875 90-91 19.555909377333094 31.127928276367218 30.368799761817478 18.94736258448221 92-93 19.68806572557243 30.99936337034533 30.136739765390487 19.17583113869176 94-95 19.463180543245834 31.10721885855113 30.180544941818066 19.249055656384968 96-97 19.383907943578503 31.37145104938794 29.787298594630187 19.45734241240337 98-99 19.185291789178425 31.44976998444363 30.081271600826458 19.28366662555148 100-101 19.62020853232798 31.798849928784804 29.004565407410155 19.576376131477062 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17896.0 1 29201.5 2 36968.5 3 39114.0 4 51815.5 5 67912.0 6 84895.5 7 102755.5 8 123346.5 9 144159.5 10 164080.0 11 183079.0 12 201881.0 13 219196.5 14 235715.5 15 250512.5 16 263914.0 17 282493.5 18 309652.0 19 338704.0 20 375565.5 21 431960.5 22 506051.5 23 599643.5 24 717351.5 25 834667.0 26 958617.5 27 1105352.0 28 1404229.5 29 1619804.0 30 1736093.5 31 1918043.5 32 2093687.0 33 2154854.5 34 2035016.5 35 2055897.0 36 2262622.0 37 2401417.5 38 2453505.0 39 2278968.0 40 2106537.0 41 1979448.0 42 1763733.0 43 1665396.0 44 1513900.0 45 1406635.0 46 1388428.0 47 1353448.5 48 1303464.5 49 1275364.5 50 1189791.0 51 1047520.0 52 939502.0 53 857019.0 54 767306.0 55 668549.0 56 594507.0 57 505248.5 58 415992.5 59 344241.0 60 267192.0 61 199575.0 62 155852.0 63 119354.0 64 83520.5 65 58880.0 66 42114.5 67 30933.5 68 22719.0 69 15553.5 70 12345.0 71 12713.0 72 11432.5 73 13458.5 74 13501.0 75 8362.0 76 6192.5 77 5574.5 78 4883.0 79 2918.0 80 1617.5 81 968.0 82 637.0 83 392.5 84 272.5 85 203.0 86 203.5 87 157.5 88 82.5 89 64.0 90 50.5 91 40.5 92 33.0 93 28.5 94 22.0 95 10.0 96 4.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6423902765343178 2 0.2842004580403518 3 0.2839594503515778 4 0.30340423011106754 5 0.27549623832405074 6 0.2143658605692979 7 0.09452216767764153 8 0.1070004280855411 9 0.10299236543527807 10-11 0.08704307037927485 12-13 0.09565036309030817 14-15 0.09502339381299033 16-17 0.09144757321324584 18-19 0.10658652357655969 20-21 0.11206857527990423 22-23 0.08854412913653023 24-25 0.10528455812742195 26-27 0.09065119998077527 28-29 0.09785523415608469 30-31 0.11004620641323556 32-33 0.1059682864618786 34-35 0.10869097477201371 36-37 0.1082936613720421 38-39 0.10888482878254932 40-41 0.10356170243919342 42-43 0.0929695891608294 44-45 0.3742133369323815 46-47 0.0860886450030793 48-49 0.07210234010781497 50-51 0.07478573368608477 52-53 0.2297641561629229 54-55 0.2971284248240434 56-57 0.22140398365450925 58-59 0.25630905736234477 60-61 0.07355362553804094 62-63 0.066286719791747 64-65 0.10335300375217536 66-67 0.09962175065749693 68-69 0.07992286496527813 70-71 0.10798716246349256 72-73 0.13303275133818646 74-75 0.12132816053990195 76-77 0.1093563655649332 78-79 0.10170000536504072 80-81 0.09761335325104702 82-83 0.10451088851780689 84-85 0.0910511330295379 86-87 0.11958609409387255 88-89 0.12803271501238989 90-91 0.15421435824612348 92-93 0.16088922336564654 94-95 0.1146096346071952 96-97 0.0993414482368576 98-99 0.11632026526773229 100-101 0.10857483700894509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.7259584E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.28372085222812 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8167379113426 18.58430644863154 2 7.520382479070402 3.5020497268932504 3 3.087333286850422 2.156538192864515 4 1.7394935702242078 1.620075308533845 5 1.1716870656980356 1.3640617281939664 6 0.80176688239578 1.1200869766958732 7 0.6309006812252368 1.0282800742990281 8 0.4910178868760314 0.9146178729177952 9 0.41274284486589163 0.8649170265250725 >10 3.3624217518191544 16.001286291868038 >50 0.4962922617001986 8.097389499039828 >100 0.402574575595987 18.61956971896252 >500 0.04043199418044763 6.4811006811262475 >1k 0.023292116924593396 10.211574102507317 >5k 0.0018033078231944378 2.8543718509272535 >10k+ 0.0011213834081899223 6.579774500013841 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 219625 0.38356024381874654 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 110201 0.1924586109462479 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 94536 0.1651007454053456 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 81902 0.14303631685483428 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 81808 0.14287215219726362 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 79024 0.1380100840411275 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 75625 0.13207395988067255 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 71479 0.12483325062228885 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 65702 0.11474410991180098 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 59410 0.10375555644972902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.49116493755875 0.0 0.0 1.7464325273477363E-6 0.0 2 0.4927052910478707 0.0 0.0 3.4928650546954726E-6 0.0 3 0.4928415127850038 0.0 0.0 3.4928650546954726E-6 0.0 4 0.5059642067954947 0.0 0.0 3.4928650546954726E-6 0.0 5 0.5064287578477692 1.7464325273477363E-6 0.0 3.4928650546954726E-6 0.0 6 0.5070277842046494 1.7464325273477363E-6 0.0 3.4928650546954726E-6 0.0 7 0.5079341826863429 1.7464325273477363E-6 0.0 3.4928650546954726E-6 0.0 8 0.5138493496564698 1.7464325273477363E-6 0.0 3.4928650546954726E-6 0.0 9 0.514202129026994 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 10-11 0.5174758167995073 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 12-13 0.5201775479193143 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 14-15 0.5221929310558735 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 16-17 0.524472898720326 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 18-19 0.526727543113132 1.7464325273477363E-6 0.0 5.239297582043209E-6 0.0 20-21 0.5300544970777294 1.7464325273477363E-6 0.0 7.858946373064814E-6 0.0 22-23 0.532960560803236 2.6196487910216044E-6 0.0 8.732162636738682E-6 0.0 24-25 0.5361530394632277 3.4928650546954726E-6 0.0 9.60537890041255E-6 0.0 26-27 0.5388032508234779 3.4928650546954726E-6 0.0 1.1351811427760285E-5 0.0 28-29 0.541167047249243 3.4928650546954726E-6 0.0 1.2225027691434153E-5 0.0 30-31 0.5438207514745479 6.985730109390945E-6 0.0 1.2225027691434153E-5 0.0 32-33 0.5465521719473198 9.60537890041255E-6 1.7464325273477363E-6 1.4844676482455758E-5 0.0 34-35 0.5497821989066494 1.2225027691434153E-5 1.7464325273477363E-6 1.5717892746129624E-5 8.732162636738682E-7 36-37 0.5524123262928351 1.2225027691434153E-5 1.7464325273477363E-6 1.5717892746129624E-5 1.7464325273477363E-6 38-39 0.5557384070411688 1.3098243955108023E-5 1.7464325273477363E-6 1.5717892746129624E-5 1.7464325273477363E-6 40-41 0.5593369312637688 1.397146021878189E-5 1.7464325273477363E-6 1.5717892746129624E-5 1.7464325273477363E-6 42-43 0.5628498802925288 1.397146021878189E-5 1.7464325273477363E-6 1.7464325273477364E-5 1.7464325273477363E-6 44-45 0.5670683880623373 1.397146021878189E-5 1.7464325273477363E-6 1.7464325273477364E-5 1.7464325273477363E-6 46-47 0.573693479854831 1.5717892746129624E-5 1.7464325273477363E-6 1.8337541537151233E-5 1.7464325273477363E-6 48-49 0.5782918366993375 1.5717892746129624E-5 1.7464325273477363E-6 1.92107578008251E-5 1.7464325273477363E-6 50-51 0.5829059114365902 1.5717892746129624E-5 1.7464325273477363E-6 1.92107578008251E-5 1.7464325273477363E-6 52-53 0.5880639999061117 1.5717892746129624E-5 1.7464325273477363E-6 2.0957190328172835E-5 1.7464325273477363E-6 54-55 0.5936001910178041 1.6591109009803494E-5 1.7464325273477363E-6 2.3576839119194437E-5 1.7464325273477363E-6 56-57 0.5995258365830949 1.92107578008251E-5 1.7464325273477363E-6 2.4450055382868306E-5 1.7464325273477363E-6 58-59 0.6058461758995664 1.92107578008251E-5 1.7464325273477363E-6 2.4450055382868306E-5 1.7464325273477363E-6 60-61 0.6128825525522505 2.0083974064498965E-5 1.7464325273477363E-6 2.5323271646542176E-5 1.7464325273477363E-6 62-63 0.6203686355807265 2.0957190328172835E-5 1.7464325273477363E-6 2.6196487910216042E-5 1.7464325273477363E-6 64-65 0.6287820742812242 2.0957190328172835E-5 1.7464325273477363E-6 2.6196487910216042E-5 1.7464325273477363E-6 66-67 0.6378512983957411 2.0957190328172835E-5 1.7464325273477363E-6 2.6196487910216042E-5 1.7464325273477363E-6 68-69 0.6480015642446861 2.0957190328172835E-5 1.7464325273477363E-6 2.6196487910216042E-5 1.7464325273477363E-6 70-71 0.6592599415322333 2.0957190328172835E-5 1.7464325273477363E-6 2.6196487910216042E-5 1.7464325273477363E-6 72-73 0.6714046333274095 2.270362285552057E-5 1.7464325273477363E-6 3.056256922858538E-5 1.7464325273477363E-6 74-75 0.6845666220697657 2.270362285552057E-5 1.7464325273477363E-6 3.230900175593312E-5 3.4928650546954726E-6 76-77 0.6994549593654051 2.270362285552057E-5 1.7464325273477363E-6 3.754829933797633E-5 5.239297582043209E-6 78-79 0.7164267906661703 2.3576839119194437E-5 1.7464325273477363E-6 3.84215156016502E-5 5.239297582043209E-6 80-81 0.7353406549373465 2.6196487910216045E-5 1.7464325273477363E-6 3.929473186532406E-5 5.239297582043209E-6 82-83 0.7567589034527391 2.9689352964911517E-5 1.7464325273477363E-6 4.191438065634567E-5 5.239297582043209E-6 84-85 0.7813478002215315 2.9689352964911517E-5 1.7464325273477363E-6 4.453402944736728E-5 5.239297582043209E-6 86-87 0.8093291421746969 3.056256922858538E-5 2.6196487910216044E-6 4.715367823838888E-5 5.239297582043209E-6 88-89 0.8414067416207565 3.143578549225925E-5 3.4928650546954726E-6 4.715367823838888E-5 5.239297582043209E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 43865 0.0 66.94675 1 GCGTGTT 59670 0.0 48.936497 2 GTTTTTT 960455 0.0 48.304092 1 AGATCGG 64955 0.0 43.08762 1 CGCCGTA 44895 0.0 39.731087 46-47 GTCGCCG 44710 0.0 38.874966 44-45 AGAGCGT 70270 0.0 38.848873 9 AAGAGCG 71865 0.0 38.805943 8 CCGTATC 46640 0.0 38.7178 48-49 GATCGGA 73335 0.0 38.08719 2 ATCGGAA 75005 0.0 37.410446 3 TCGGAAG 75060 0.0 37.293133 4 CGGAAGA 77390 0.0 36.212055 5 TCGGTGG 49750 0.0 36.069473 38-39 TGGTCGC 47225 0.0 34.881435 42-43 TCTCGGT 51070 0.0 34.35691 36-37 CGTGTTT 91755 0.0 32.53939 3 GATCTCG 58590 0.0 32.20421 34-35 AGCGTCG 58470 0.0 30.8286 10-11 GGTGGTC 53965 0.0 30.756569 40-41 >>END_MODULE