FastQCFastQC Report
Fri 27 May 2016
SRR1233082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57259584
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2310440.40350275684853043TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1175300.20525821493917942TruSeq Adapter, Index 7 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT914970.15979333695473583No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT866350.15130218200677112No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT838020.14635453865679499No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG772550.13492064490024935No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA753180.1315378050947768No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT741100.12942811460174072No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT703270.12282136035078424No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT615520.10749641492330787No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT577190.10080233904598399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT464000.070.0089651
GCGTGTT625800.052.4399262
GTTTTTT8985900.045.849191
AGATCGG620000.044.5064051
TATGCCG565650.038.04351846-47
GATCGGA766450.036.9778332
TTCGTAT144600.036.6424521
TGCCGTC571700.036.5701248-49
CCGTCTT578000.036.4649450-51
ATCGGAA799750.035.448873
TCGGAAG799900.035.4006924
CTCGTAT595050.035.37817442-43
CGTATGC620350.034.70868744-45
CGGAAGA821050.034.5731775
CGTGTTT983500.033.9791953
ATCTCGT634150.032.24076540-41
CGTCTGA699950.031.69589816-17
CACGTCT707100.031.28128414-15
CACACGT728150.030.50039112-13
AGAGCAC980400.029.0353499
Produced by FastQC (version 0.11.5)