##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57259584 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.219298938671997 31.0 31.0 34.0 26.0 34.0 2 31.17473260022287 33.0 31.0 34.0 27.0 34.0 3 31.357381883878165 34.0 31.0 34.0 28.0 34.0 4 34.78597163751661 37.0 35.0 37.0 32.0 37.0 5 34.595761540985 37.0 35.0 37.0 32.0 37.0 6 34.59304391732919 37.0 35.0 37.0 31.0 37.0 7 34.65577446388713 37.0 35.0 37.0 32.0 37.0 8 34.59893505338774 37.0 35.0 37.0 32.0 37.0 9 36.19110369366288 39.0 37.0 39.0 32.0 39.0 10-11 36.07751043912579 39.0 37.0 39.0 31.5 39.0 12-13 36.01848057436114 39.0 37.0 39.0 31.0 39.0 14-15 37.29409961308835 40.0 38.0 41.0 31.0 41.0 16-17 37.0869222347127 40.0 37.5 41.0 31.0 41.0 18-19 36.65521417340371 40.0 36.5 41.0 30.5 41.0 20-21 36.245339199460474 40.0 36.0 41.0 29.5 41.0 22-23 35.91205064465714 40.0 35.5 41.0 27.5 41.0 24-25 35.45855410860128 40.0 35.0 41.0 25.0 41.0 26-27 34.93801318919816 40.0 35.0 41.0 17.5 41.0 28-29 34.48691104706594 40.0 35.0 41.0 9.0 41.0 30-31 34.07406754823786 40.0 35.0 41.0 4.5 41.0 32-33 33.89599111478002 40.0 34.5 41.0 2.0 41.0 34-35 33.88029264236359 40.0 35.0 41.0 2.0 41.0 36-37 33.79136391385589 40.0 35.0 41.0 2.0 41.0 38-39 33.683851056270335 40.0 35.0 41.0 2.0 41.0 40-41 33.468082504755884 40.0 34.0 41.0 2.0 41.0 42-43 33.46079850143515 40.0 34.0 41.0 2.0 41.0 44-45 33.36447303738707 40.0 34.0 41.0 2.0 41.0 46-47 33.23817693995122 40.0 34.0 41.0 2.0 41.0 48-49 33.14192443312197 40.0 34.0 41.0 2.0 41.0 50-51 32.99454207526202 39.0 33.0 41.0 2.0 41.0 52-53 32.823732634872094 39.0 33.0 41.0 2.0 41.0 54-55 32.670068586247496 39.0 33.0 41.0 2.0 41.0 56-57 32.51344179342972 39.0 33.0 41.0 2.0 41.0 58-59 32.44158955119199 39.0 33.0 41.0 2.0 41.0 60-61 32.32878909843285 39.0 33.0 41.0 2.0 41.0 62-63 32.13428324942075 38.5 32.5 41.0 2.0 41.0 64-65 31.70339128729961 38.0 32.0 40.0 2.0 41.0 66-67 31.5195380741851 37.5 31.5 40.0 2.0 41.0 68-69 31.130934403924414 37.0 31.0 40.0 2.0 41.0 70-71 30.84350949877666 36.5 31.0 39.5 2.0 41.0 72-73 30.50319829428031 36.0 31.0 39.0 2.0 41.0 74-75 30.176182706811144 36.0 30.0 39.0 2.0 41.0 76-77 29.052473503824267 34.5 28.5 37.0 2.0 39.0 78-79 29.469668344080183 35.0 30.0 37.0 2.0 39.0 80-81 29.288470974570824 35.0 30.0 37.0 2.0 39.0 82-83 28.978560191775056 35.0 30.0 36.5 2.0 39.0 84-85 28.67542911244343 35.0 29.0 36.0 2.0 38.0 86-87 28.359937447327596 35.0 29.0 36.0 2.0 37.0 88-89 28.083220234362862 35.0 29.0 35.5 2.0 37.0 90-91 27.82695778055251 35.0 29.0 35.0 2.0 36.0 92-93 27.58599915780038 34.5 29.0 35.0 2.0 36.0 94-95 27.36288136497813 34.0 28.0 35.0 2.0 36.0 96-97 27.11683973289083 34.0 27.0 35.0 2.0 36.0 98-99 26.836561840896366 34.0 26.5 35.0 2.0 35.5 100-101 25.778047479352978 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5027485911895546 1101 2 0.8873647236477566 1101 3 0.8132760970839286 1101 4 0.6963421439714494 1101 5 0.7211255459589907 1101 6 0.6735196107729848 1101 7 0.6863998976524357 1101 8 0.7027284911658356 1101 9 0.8002782464761538 1101 10-11 0.7073751929941423 1101 12-13 0.814675460414982 1101 14-15 0.9173817043812775 1101 16-17 0.9447920497134277 1101 18-19 0.9179697068475008 1101 20-21 0.8811691478024031 1101 22-23 0.9207911002428943 1101 24-25 0.9487423358054698 1101 26-27 0.914208579545857 1101 28-29 0.90973670128691 1101 30-31 1.0416563988212957 1101 32-33 0.9209930510565982 1101 34-35 0.9024398965339486 1101 36-37 0.9137420251127608 1101 38-39 0.9440498429784014 1101 40-41 1.0133214744775998 1101 42-43 0.959540990567298 1101 44-45 0.9071880599959385 1101 46-47 0.940078757401281 1101 48-49 0.9416900643348569 1101 50-51 0.8496636789874756 1101 52-53 0.8405919830468136 1101 54-55 0.8272405470730178 1101 56-57 0.8365548427882459 1101 58-59 0.8930025332394251 1101 60-61 0.8649416821435949 1101 62-63 0.8785841798592955 1101 64-65 0.7281689434864482 1101 66-67 0.7689962524010845 1101 68-69 0.6828112946453615 1101 70-71 0.7493189249127781 1101 72-73 0.7503626013851452 1101 74-75 0.7813894420630803 1101 76-77 0.7526618696806331 1101 78-79 0.7700526422180474 1101 80-81 0.7058670212919012 1101 82-83 0.7483534470161217 1101 84-85 0.7591562551047275 1101 86-87 0.8257592351074088 1101 88-89 0.7630039326759004 1101 90-91 0.7775120520981602 1101 92-93 0.8374460922956999 1101 94-95 0.8954466804038681 1101 96-97 0.8851805691820296 1101 98-99 0.8368047585617582 1101 100-101 0.8430258444629253 1102 1 1.4693752627696384 1102 2 0.858662118347624 1102 3 0.7749492724868645 1102 4 0.6970904310504267 1102 5 0.7025401803853129 1102 6 0.6959328771382687 1102 7 0.6569768574842101 1102 8 0.6348762398329342 1102 9 0.7172013028146438 1102 10-11 0.7619718292915607 1102 12-13 0.7574580292036828 1102 14-15 0.8832800868443158 1102 16-17 0.8925087271056427 1102 18-19 0.9026263456967456 1102 20-21 0.7961323280956094 1102 22-23 0.8320536948969846 1102 24-25 0.8671983363183244 1102 26-27 0.8857530305863719 1102 28-29 0.8746769160341614 1102 30-31 0.9760014614441062 1102 32-33 0.8760586296814168 1102 34-35 0.8273886422322363 1102 36-37 0.8124286421746163 1102 38-39 0.8545836245778915 1102 40-41 0.9190870562895768 1102 42-43 0.8038581205570026 1102 44-45 0.8366156918918151 1102 46-47 0.8480005608068382 1102 48-49 0.8703115131094208 1102 50-51 0.8400480053361292 1102 52-53 0.814171022623924 1102 54-55 0.8172230267098612 1102 56-57 0.7984543140031661 1102 58-59 0.8943484888502695 1102 60-61 0.8376276388557358 1102 62-63 0.8803131459673068 1102 64-65 0.7688938282024971 1102 66-67 0.82956066959051 1102 68-69 0.7624371909611263 1102 70-71 0.7769191500966599 1102 72-73 0.7722670722580105 1102 74-75 0.700030866653119 1102 76-77 0.6327123477886225 1102 78-79 0.7289167583148064 1102 80-81 0.8015678793785419 1102 82-83 0.7824047020755742 1102 84-85 0.7615459091971317 1102 86-87 0.7396406949189434 1102 88-89 0.7350526651403264 1102 90-91 0.7440378894561945 1102 92-93 0.7428249841161438 1102 94-95 0.7264834193702789 1102 96-97 0.7600621746787652 1102 98-99 0.7929152022298602 1102 100-101 0.8226405463493016 1103 1 1.4125627810551435 1103 2 0.8301798743702165 1103 3 0.7308110135066954 1103 4 0.6507071661147705 1103 5 0.6008199439381343 1103 6 0.6117219390992616 1103 7 0.5825797774430228 1103 8 0.6229677342740487 1103 9 0.691069762337662 1103 10-11 0.7118300753086828 1103 12-13 0.7564790306678475 1103 14-15 0.898462672094837 1103 16-17 0.9368926599923171 1103 18-19 0.891655992617693 1103 20-21 0.9077278968508296 1103 22-23 0.916320707985534 1103 24-25 0.8391989081751916 1103 26-27 0.8134632255555161 1103 28-29 0.7994617016237555 1103 30-31 0.8435361324540835 1103 32-33 0.8544694458763971 1103 34-35 0.7893481072750603 1103 36-37 0.7642331149354575 1103 38-39 0.8007613101728737 1103 40-41 0.9014402080182151 1103 42-43 0.8178172405783073 1103 44-45 0.8281650521503465 1103 46-47 0.8519551465356585 1103 48-49 0.8492767992735608 1103 50-51 0.8340423916743873 1103 52-53 0.8510500525654052 1103 54-55 0.8365182098148196 1103 56-57 0.8434126539837123 1103 58-59 0.7751028890062912 1103 60-61 0.7949232442438472 1103 62-63 0.8158436102017106 1103 64-65 0.8050527132639154 1103 66-67 0.8279689047638392 1103 68-69 0.7934563269188608 1103 70-71 0.8622195527082717 1103 72-73 0.8219237257557914 1103 74-75 0.815391431334298 1103 76-77 0.740940410628653 1103 78-79 0.7694124659780073 1103 80-81 0.7434207813046108 1103 82-83 0.778539558767001 1103 84-85 0.7638988309516712 1103 86-87 0.7602876187014829 1103 88-89 0.7550124311413775 1103 90-91 0.7279188237160135 1103 92-93 0.7146943198344466 1103 94-95 0.6784975427980413 1103 96-97 0.779729496318911 1103 98-99 0.7940561665142845 1103 100-101 0.8401860188986916 1104 1 1.375249235139698 1104 2 0.7380051718791378 1104 3 0.6463971889831157 1104 4 0.5819322493998413 1104 5 0.5941968162074716 1104 6 0.5498501517930734 1104 7 0.5394266780149977 1104 8 0.543953999821774 1104 9 0.6652283148947049 1104 10-11 0.6308125976993963 1104 12-13 0.6925805753532686 1104 14-15 0.8052674432939355 1104 16-17 0.7753986837418267 1104 18-19 0.7387898074683363 1104 20-21 0.7120050287046809 1104 22-23 0.7379940698279341 1104 24-25 0.772424330478998 1104 26-27 0.7708508716886513 1104 28-29 0.7388182904414364 1104 30-31 0.7431323204815143 1104 32-33 0.7029435008507079 1104 34-35 0.726200448029438 1104 36-37 0.7269363367580013 1104 38-39 0.7359079657151142 1104 40-41 0.8188533862614307 1104 42-43 0.7140183223124197 1104 44-45 0.7222972767142295 1104 46-47 0.7488083476378833 1104 48-49 0.682336159649239 1104 50-51 0.7099876526865927 1104 52-53 0.7340574218386919 1104 54-55 0.7205208963015579 1104 56-57 0.7201412504414151 1104 58-59 0.7415652050462853 1104 60-61 0.7135750593167955 1104 62-63 0.7579845407437915 1104 64-65 0.7386911803359233 1104 66-67 0.7670500718913011 1104 68-69 0.6611452361451917 1104 70-71 0.6601322368455342 1104 72-73 0.7107305634914027 1104 74-75 0.669297939072397 1104 76-77 0.5729355742227931 1104 78-79 0.6939044830438306 1104 80-81 0.7055836711063215 1104 82-83 0.7000507254174622 1104 84-85 0.6729987252854137 1104 86-87 0.6922715354551308 1104 88-89 0.721262722104747 1104 90-91 0.7327767275421095 1104 92-93 0.6581996674704307 1104 94-95 0.7315181440586933 1104 96-97 0.7136916807657023 1104 98-99 0.7222431184914271 1104 100-101 0.8146091816917505 1105 1 1.2597781779712882 1105 2 0.6592699120253833 1105 3 0.5715956548017118 1105 4 0.46877090707039315 1105 5 0.42421155120210585 1105 6 0.4175572078829646 1105 7 0.41737348773754945 1105 8 0.39547098421533633 1105 9 0.40408677527390324 1105 10-11 0.4471828584603301 1105 12-13 0.4932908375622418 1105 14-15 0.5885349715650108 1105 16-17 0.5986209672466956 1105 18-19 0.5675347480388311 1105 20-21 0.5944763404559765 1105 22-23 0.5963875802134311 1105 24-25 0.6087555897701336 1105 26-27 0.5938729453032465 1105 28-29 0.5716384044061442 1105 30-31 0.6393173350251402 1105 32-33 0.6648053800271185 1105 34-35 0.5153661616899825 1105 36-37 0.5554526579647572 1105 38-39 0.5834039767498282 1105 40-41 0.6674011794020842 1105 42-43 0.5641648186714434 1105 44-45 0.527236215018057 1105 46-47 0.5937447722657438 1105 48-49 0.5894629021140929 1105 50-51 0.5884561777228896 1105 52-53 0.5733185070385005 1105 54-55 0.503214328486834 1105 56-57 0.5338046800774947 1105 58-59 0.5552986863015548 1105 60-61 0.5261731246234831 1105 62-63 0.5110090590896448 1105 64-65 0.47076290818046473 1105 66-67 0.485468202869459 1105 68-69 0.46132598401981184 1105 70-71 0.4894005134421846 1105 72-73 0.5006825571619125 1105 74-75 0.5001864774479508 1105 76-77 0.3986852794358917 1105 78-79 0.4936315621140679 1105 80-81 0.5546461386269641 1105 82-83 0.5402365399403841 1105 84-85 0.5753675470163238 1105 86-87 0.5471595913382501 1105 88-89 0.5693042780075181 1105 90-91 0.5861160188393519 1105 92-93 0.60329826671747 1105 94-95 0.6563917568569018 1105 96-97 0.6497489112712209 1105 98-99 0.7076777672573193 1105 100-101 0.6757700828549282 1106 1 1.1587607192332712 1106 2 0.5366683174644216 1106 3 0.5140221671642671 1106 4 0.48106932899899135 1106 5 0.31356236237150625 1106 6 0.2634740705902985 1106 7 0.340123347280354 1106 8 0.33240643376096557 1106 9 0.3749810795877835 1106 10-11 0.3710265885151003 1106 12-13 0.41677156920849256 1106 14-15 0.47135404618180843 1106 16-17 0.5045311563512485 1106 18-19 0.4710657296208254 1106 20-21 0.4383271167159677 1106 22-23 0.4579105974515656 1106 24-25 0.3590312112085243 1106 26-27 0.38721735544176994 1106 28-29 0.3731035239518974 1106 30-31 0.4439768892204867 1106 32-33 0.4118196296870451 1106 34-35 0.3146888457033441 1106 36-37 0.3766416857984467 1106 38-39 0.39042081752353397 1106 40-41 0.4821381421421975 1106 42-43 0.38588204224431166 1106 44-45 0.34997225603679993 1106 46-47 0.3911345996144817 1106 48-49 0.3873139022120924 1106 50-51 0.40265055248249837 1106 52-53 0.4236969143830649 1106 54-55 0.41881102151032934 1106 56-57 0.43224578231072286 1106 58-59 0.45736914124826455 1106 60-61 0.43002957178792656 1106 62-63 0.46967031017430827 1106 64-65 0.46732135597504154 1106 66-67 0.5399963313121994 1106 68-69 0.45391074805488074 1106 70-71 0.4781971918384045 1106 72-73 0.45381421675344313 1106 74-75 0.44536236479360625 1106 76-77 0.3300901230177793 1106 78-79 0.45673208793042264 1106 80-81 0.4558422897408114 1106 82-83 0.49703555437619684 1106 84-85 0.4795152622419998 1106 86-87 0.42906202590538456 1106 88-89 0.4597052472816934 1106 90-91 0.4576601421935145 1106 92-93 0.45487942620021826 1106 94-95 0.46995925274960015 1106 96-97 0.49838130350517673 1106 98-99 0.5420513010561407 1106 100-101 0.47081755775668555 1107 1 1.2051133055971697 1107 2 0.6060709280560985 1107 3 0.5292738067634915 1107 4 0.4754855933958595 1107 5 0.40719733993895346 1107 6 0.40491871988901096 1107 7 0.36172655702658574 1107 8 0.40789324960919515 1107 9 0.4547445678375155 1107 10-11 0.4356761750372087 1107 12-13 0.45541680704040033 1107 14-15 0.4867065797107202 1107 16-17 0.4928580281935453 1107 18-19 0.4641044311268061 1107 20-21 0.41889333656071415 1107 22-23 0.41060079993414433 1107 24-25 0.34639908021421917 1107 26-27 0.3316656405410754 1107 28-29 0.3260724922942728 1107 30-31 0.3021179230450812 1107 32-33 0.3075231203856248 1107 34-35 0.27089354540177624 1107 36-37 0.2505089156310376 1107 38-39 0.24094237217544645 1107 40-41 0.3064352929448333 1107 42-43 0.2991472492545526 1107 44-45 0.3166484027692178 1107 46-47 0.2885917421440425 1107 48-49 0.2871405259402593 1107 50-51 0.20507421403426918 1107 52-53 0.18618908488894448 1107 54-55 0.16927651579351988 1107 56-57 0.20257619077090538 1107 58-59 0.2774279888464406 1107 60-61 0.2220080732646892 1107 62-63 0.27473951695500887 1107 64-65 0.27483785342445444 1107 66-67 0.2748638368494163 1107 68-69 0.2806288292548622 1107 70-71 0.29950289529205065 1107 72-73 0.2988846024687639 1107 74-75 0.2947452154454915 1107 76-77 0.15550906054141578 1107 78-79 0.24139293373229265 1107 80-81 0.26805463654288175 1107 82-83 0.3205984888320721 1107 84-85 0.298464335621059 1107 86-87 0.3102900173049896 1107 88-89 0.2895544359171218 1107 90-91 0.2510372293335337 1107 92-93 0.2716873864485727 1107 94-95 0.2713353769297022 1107 96-97 0.28554556865015357 1107 98-99 0.2935842057306708 1107 100-101 0.3050714633379066 1108 1 1.0773298077827924 1108 2 0.5313705850947059 1108 3 0.42543573443612104 1108 4 0.3903286714912966 1108 5 0.30794220964709496 1108 6 0.2980368025025655 1108 7 0.30359188630007594 1108 8 0.2862319705828611 1108 9 0.33779545489227303 1108 10-11 0.3087376835096336 1108 12-13 0.327527473867562 1108 14-15 0.300197329120202 1108 16-17 0.30206982990406317 1108 18-19 0.3175573491032537 1108 20-21 0.3204722719832489 1108 22-23 0.29011306710511064 1108 24-25 0.36802894131083974 1108 26-27 0.34785128449828306 1108 28-29 0.33311582340476775 1108 30-31 0.37531316573446105 1108 32-33 0.35559318083188884 1108 34-35 0.29522604234018246 1108 36-37 0.28641450757560705 1108 38-39 0.3072557533746334 1108 40-41 0.40309331719535635 1108 42-43 0.3295156816544278 1108 44-45 0.3363226968296047 1108 46-47 0.1802070913331022 1108 48-49 0.3054336278009302 1108 50-51 0.24196953233649765 1108 52-53 0.28034813446763707 1108 54-55 0.2874872977464733 1108 56-57 0.26288005991455776 1108 58-59 0.3595598906429842 1108 60-61 0.3362633603176306 1108 62-63 0.3570466084178321 1108 64-65 0.2995013914927718 1108 66-67 0.3202358966708978 1108 68-69 0.16185706517157072 1108 70-71 0.2395510357372963 1108 72-73 0.28518995922347656 1108 74-75 0.2792729835073473 1108 76-77 0.18482563890707127 1108 78-79 0.24518120740380311 1108 80-81 0.25600554932671926 1108 82-83 0.3057721369090487 1108 84-85 0.2748214656481842 1108 86-87 0.261294423148545 1108 88-89 0.2661119365365465 1108 90-91 0.26704308154027245 1108 92-93 0.25141759140509734 1108 94-95 0.29202593763881524 1108 96-97 0.3132239986211829 1108 98-99 0.3471877961463825 1108 100-101 0.24912655913288262 1109 1 1.0368665462964834 1109 2 0.4413658905066953 1109 3 0.36602713965045197 1109 4 0.35337571211837115 1109 5 0.26048994607485554 1109 6 0.24365323496186875 1109 7 0.22418384494104515 1109 8 0.26494617001150544 1109 9 0.22862938119014586 1109 10-11 0.25047911166476666 1109 12-13 0.22536204804517723 1109 14-15 0.1991808917922171 1109 16-17 0.1886139568184717 1109 18-19 0.18946026824430362 1109 20-21 0.18915934504676102 1109 22-23 0.1750983911514794 1109 24-25 0.18842494332815818 1109 26-27 0.13838938060970207 1109 28-29 0.1146468390163875 1109 30-31 0.23034087224954902 1109 32-33 0.18335821535653452 1109 34-35 0.10149731627267755 1109 36-37 0.12522878523497383 1109 38-39 0.16365051353387372 1109 40-41 0.2768683875519784 1109 42-43 0.10052067747171378 1109 44-45 0.16991796004771942 1109 46-47 0.13059608183568372 1109 48-49 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-4.059520169080507 2316 84-85 -4.027964295647589 2316 86-87 -3.9759748670147985 2316 88-89 -3.9705633762415253 2316 90-91 -4.042705357732217 2316 92-93 -4.0354605505619325 2316 94-95 -3.988067805548564 2316 96-97 -3.9444534730131195 2316 98-99 -3.8904969899259214 2316 100-101 -3.7155750626799424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1119810.0 3 364507.0 4 204776.0 5 208116.0 6 546311.0 7 534786.0 8 433500.0 9 714690.0 10 1058008.0 11 906475.0 12 598933.0 13 425351.0 14 366583.0 15 331014.0 16 294617.0 17 263592.0 18 245224.0 19 235733.0 20 234319.0 21 243948.0 22 268195.0 23 305344.0 24 349677.0 25 400415.0 26 465109.0 27 558715.0 28 601664.0 29 664715.0 30 790379.0 31 958255.0 32 1185330.0 33 1514457.0 34 2031581.0 35 2921040.0 36 4644259.0 37 9035427.0 38 1.5261063E7 39 5950248.0 40 23418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.854501012741345 17.972593857565926 11.05687372752085 43.11603140217188 2 13.501891392163662 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32.94290910327785 18.34747153667935 46-47 19.161486587175578 29.395532516304556 33.049577431653724 18.393403464866143 48-49 18.943123455314424 30.097387534352176 32.56215051787122 18.39733849246218 50-51 18.645549700536122 29.606219973754367 32.616316830381514 19.131913495328 52-53 19.144904609730318 29.993362208544738 31.888242911142257 18.973490270582694 54-55 18.848785966935313 30.307034486941664 31.44897688031168 19.395202665811347 56-57 19.073999057177566 30.341594898759194 31.554218975409604 19.030187068653635 58-59 19.324388832363766 30.002069891554967 31.370317152722283 19.303224123358987 60-61 19.6469515385366 30.77720543800192 30.13968281287575 19.436160210585733 62-63 19.569083886976564 30.44076504872189 31.164650000683043 18.825501063618503 64-65 20.23270204046039 30.437237579131637 30.485229445997376 18.8448309344106 66-67 19.616024619753528 32.16998636092978 29.325950997608963 18.888038021707736 68-69 19.739256132641408 31.375047691386467 30.471519864803952 18.414176311168173 70-71 19.970812889827634 31.34380258095981 29.94310667885013 18.74227785036243 72-73 19.18815490243544 32.33009718657328 29.614470330612598 18.867277580378676 74-75 19.18492096563787 30.95372015859527 30.768141897497 19.09321697826986 76-77 19.387168248135303 31.450045195487615 30.181570127552 18.98121642882508 78-79 19.276929141173223 31.5020438641242 29.92682005470782 19.294206939994755 80-81 19.332342886398287 30.879968861153316 30.317435925179637 19.47025232726876 82-83 19.65223085277432 30.921498211404803 30.19183913681276 19.23443179900812 84-85 19.451270358669785 30.963280614251136 30.193115438010693 19.392333589068382 86-87 19.822016646491658 30.944829916892264 29.915657288831987 19.31749614778409 88-89 19.622516269722457 31.1487849826176 29.878175338369473 19.35052340929047 90-91 19.61524381294197 31.259862398171727 30.09134341375806 19.033550375128243 92-93 19.740868885846222 31.14241187180261 29.847942382902758 19.26877685944841 94-95 19.44278230989748 31.23275349487112 30.00339692424316 19.321067270988245 96-97 19.356177773282887 31.47710025954883 29.607639905588528 19.559082061579755 98-99 19.153894925040312 31.61939132064953 29.845370524140723 19.38134323016943 100-101 19.40697014096203 31.966878991306586 29.05737841880867 19.568772448922708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17757.0 1 23856.5 2 27337.5 3 30243.0 4 42950.5 5 58975.5 6 75715.0 7 93289.0 8 113171.0 9 133208.0 10 153124.0 11 171889.5 12 190086.5 13 207834.0 14 224840.0 15 240254.0 16 254021.0 17 272394.5 18 298517.5 19 325772.0 20 362644.0 21 420395.5 22 495260.5 23 589096.0 24 706778.0 25 825862.5 26 949948.0 27 1095481.0 28 1400562.0 29 1623333.0 30 1744848.5 31 1924812.0 32 2096349.0 33 2157306.5 34 2034325.0 35 2053922.5 36 2257557.5 37 2409552.0 38 2464513.5 39 2274746.0 40 2113380.0 41 1995142.5 42 1768629.5 43 1669538.0 44 1526702.5 45 1422864.0 46 1404933.0 47 1370695.0 48 1322865.5 49 1287956.5 50 1195170.0 51 1056755.0 52 950983.5 53 867515.0 54 777138.0 55 675711.0 56 603161.0 57 515903.0 58 424168.0 59 349060.5 60 269694.0 61 201195.0 62 157405.5 63 121034.0 64 84874.5 65 60482.0 66 43677.5 67 32624.0 68 24646.0 69 17439.0 70 14256.5 71 14855.0 72 13978.5 73 16976.5 74 16677.0 75 9779.0 76 6525.0 77 6262.0 78 5439.5 79 2930.0 80 1987.5 81 1381.0 82 998.0 83 671.0 84 490.5 85 380.5 86 323.0 87 253.5 88 153.0 89 106.0 90 74.0 91 40.5 92 22.5 93 15.5 94 8.5 95 2.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05112681223810498 2 0.007228484230692281 3 0.006285410665924503 4 0.00395741610696997 5 0.03819447937309499 6 0.1201964722621806 7 0.13576941110854038 8 0.14460461326439256 9 0.14712471540135533 10-11 0.1580503972924428 12-13 0.1639437338559777 14-15 0.16685765652785742 16-17 0.1763416583676193 18-19 0.17446424340072048 20-21 0.17743055904842062 22-23 0.1502473367602531 24-25 0.17015579435575362 26-27 0.1761146221390641 28-29 0.1780418104329923 30-31 0.19164390017224014 32-33 0.20049394700457482 34-35 0.19823668296297786 36-37 0.19204819930232114 38-39 0.22128697267517697 40-41 0.24517380356797563 42-43 0.2562750019280615 44-45 0.2891708050131835 46-47 0.2732372278499264 48-49 0.26773159930746265 50-51 0.26244602126344474 52-53 0.25841525499032614 54-55 0.2599844246161481 56-57 0.27529627179966937 58-59 0.2667282738205014 60-61 0.2744344073474233 62-63 0.2829989124615366 64-65 0.3873491291868275 66-67 0.18534539126236055 68-69 0.32642395725403806 70-71 0.00668883657974183 72-73 0.00409276462783942 74-75 0.015848001969417033 76-77 0.034770598403229755 78-79 0.01714036203965436 80-81 0.02453213771165365 82-83 0.02926060377944765 84-85 0.023555008712602594 86-87 0.019848205673307022 88-89 0.029523441874813483 90-91 0.021239239181339492 92-93 0.021430473543084073 94-95 0.031602569798620965 96-97 0.033698288831438244 98-99 0.018008339005746183 100-101 0.028957597735952814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.7259584E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.85257225373697 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1455181768762 17.07680582265903 2 7.808308782526646 3.4126326369930493 3 3.360726850940359 2.2032157900574436 4 1.943567456816699 1.698877931203949 5 1.3336493848146138 1.4571834771406604 6 0.9220392492075553 1.2089357588453695 7 0.6965104768229399 1.0654381864180658 8 0.5468489081821516 0.9560044222342113 9 0.46344121800599625 0.9114644431652309 >10 3.7160020378548806 16.729154089722083 >50 0.5509220773650734 8.463371194847873 >100 0.44163375307168734 19.156071456867096 >500 0.043511693505673084 6.520023358896725 >1k 0.02432941945527551 10.011386658997512 >5k 0.0018497194689695172 2.7471528917762207 >10k+ 0.0011407950851849706 6.38228188017539 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG 231044 0.40350275684853043 TruSeq Adapter, Index 7 (100% over 49bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 117530 0.20525821493917942 TruSeq Adapter, Index 7 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91497 0.15979333695473583 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 86635 0.15130218200677112 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 83802 0.14635453865679499 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 77255 0.13492064490024935 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 75318 0.1315378050947768 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 74110 0.12942811460174072 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 70327 0.12282136035078424 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 61552 0.10749641492330787 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 57719 0.10080233904598399 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.4906672042884559 0.0 0.0 1.7464325273477363E-6 0.0 2 0.49228964010636195 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 3 0.49259002650106576 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 4 0.4972530013490842 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 5 0.4978450419758551 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 6 0.4985663186096497 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 7 0.4994011133577219 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 8 0.5057197062416661 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 9 0.5062314109721789 3.4928650546954726E-6 0.0 3.4928650546954726E-6 0.0 10-11 0.5093601448449223 5.239297582043209E-6 0.0 3.4928650546954726E-6 0.0 12-13 0.5122854193282298 5.239297582043209E-6 0.0 5.239297582043209E-6 0.0 14-15 0.5146081745896023 6.1125138457170766E-6 0.0 5.239297582043209E-6 0.0 16-17 0.517080249832063 7.858946373064814E-6 0.0 5.239297582043209E-6 0.0 18-19 0.519382921119371 8.732162636738682E-6 0.0 5.239297582043209E-6 0.0 20-21 0.52253435861497 1.0478595164086417E-5 0.0 6.985730109390945E-6 0.0 22-23 0.5252142593281851 1.0478595164086417E-5 0.0 6.985730109390945E-6 0.0 24-25 0.5281430266765472 1.0478595164086417E-5 0.0 8.732162636738682E-6 0.0 26-27 0.530403783583199 1.0478595164086417E-5 1.7464325273477363E-6 1.0478595164086417E-5 0.0 28-29 0.5323536754999827 1.0478595164086417E-5 1.7464325273477363E-6 1.2225027691434153E-5 0.0 30-31 0.5344939285622474 1.0478595164086417E-5 1.7464325273477363E-6 1.2225027691434153E-5 0.0 32-33 0.5367660372803267 1.1351811427760285E-5 1.7464325273477363E-6 1.3098243955108023E-5 0.0 34-35 0.5395131756458447 1.2225027691434153E-5 1.7464325273477363E-6 1.397146021878189E-5 0.0 36-37 0.5416438233292089 1.2225027691434153E-5 1.7464325273477363E-6 1.397146021878189E-5 0.0 38-39 0.5439700714556361 1.2225027691434153E-5 1.7464325273477363E-6 1.92107578008251E-5 0.0 40-41 0.5465154968642454 1.2225027691434153E-5 1.7464325273477363E-6 1.92107578008251E-5 0.0 42-43 0.5493447175585489 1.397146021878189E-5 3.4928650546954726E-6 2.6196487910216045E-5 0.0 44-45 0.5522926956647118 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 0.0 46-47 0.5557017319580946 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 0.0 48-49 0.5592129345543272 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 0.0 50-51 0.5629572858929608 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 0.0 52-53 0.5673320993739668 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 0.0 54-55 0.5719671313015477 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 8.732162636738682E-7 56-57 0.577093783985577 1.397146021878189E-5 3.4928650546954726E-6 2.9689352964911517E-5 1.7464325273477363E-6 58-59 0.5825330480920015 1.397146021878189E-5 3.4928650546954726E-6 3.230900175593312E-5 1.7464325273477363E-6 60-61 0.5886595333979374 1.397146021878189E-5 3.4928650546954726E-6 3.405543428328086E-5 1.7464325273477363E-6 62-63 0.5953876647095445 1.397146021878189E-5 3.4928650546954726E-6 3.84215156016502E-5 1.7464325273477363E-6 64-65 0.6031121357780036 1.397146021878189E-5 3.4928650546954726E-6 3.84215156016502E-5 1.7464325273477363E-6 66-67 0.6114932654767453 1.397146021878189E-5 3.4928650546954726E-6 4.016794812899793E-5 1.7464325273477363E-6 68-69 0.6208724813648664 1.397146021878189E-5 3.4928650546954726E-6 4.366081318369341E-5 1.7464325273477363E-6 70-71 0.6313894980445545 1.397146021878189E-5 3.4928650546954726E-6 4.366081318369341E-5 1.7464325273477363E-6 72-73 0.6429735849984519 1.397146021878189E-5 3.4928650546954726E-6 4.366081318369341E-5 2.6196487910216044E-6 74-75 0.655742626422155 1.5717892746129624E-5 3.4928650546954726E-6 4.802689450206274E-5 3.4928650546954726E-6 76-77 0.6700834571204708 1.5717892746129624E-5 3.4928650546954726E-6 5.413940834777982E-5 3.4928650546954726E-6 78-79 0.6865593015834695 1.5717892746129624E-5 3.4928650546954726E-6 5.588584087512756E-5 5.239297582043209E-6 80-81 0.7051465829720314 1.5717892746129624E-5 3.4928650546954726E-6 6.199835472084464E-5 5.239297582043209E-6 82-83 0.726230389658437 1.5717892746129624E-5 3.4928650546954726E-6 6.636443603921398E-5 5.239297582043209E-6 84-85 0.7505468429529631 1.5717892746129624E-5 5.239297582043209E-6 7.073051735758331E-5 5.239297582043209E-6 86-87 0.7783832310063586 1.5717892746129624E-5 5.239297582043209E-6 7.160373362125718E-5 5.239297582043209E-6 88-89 0.810389226718797 1.5717892746129624E-5 5.239297582043209E-6 7.335016614860492E-5 5.239297582043209E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 46400 0.0 70.008965 1 GCGTGTT 62580 0.0 52.439926 2 GTTTTTT 898590 0.0 45.84919 1 AGATCGG 62000 0.0 44.506405 1 TATGCCG 56565 0.0 38.043518 46-47 GATCGGA 76645 0.0 36.977833 2 TTCGTAT 14460 0.0 36.642452 1 TGCCGTC 57170 0.0 36.57012 48-49 CCGTCTT 57800 0.0 36.46494 50-51 ATCGGAA 79975 0.0 35.44887 3 TCGGAAG 79990 0.0 35.400692 4 CTCGTAT 59505 0.0 35.378174 42-43 CGTATGC 62035 0.0 34.708687 44-45 CGGAAGA 82105 0.0 34.573177 5 CGTGTTT 98350 0.0 33.979195 3 ATCTCGT 63415 0.0 32.240765 40-41 CGTCTGA 69995 0.0 31.695898 16-17 CACGTCT 70710 0.0 31.281284 14-15 CACACGT 72815 0.0 30.500391 12-13 AGAGCAC 98040 0.0 29.035349 9 >>END_MODULE