FastQCFastQC Report
Fri 27 May 2016
SRR1233081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113110132
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCCATGGCTTCGTCCAGACAGCGCAGCTGCAGTATGGCTGGACAGA1534780.1356889938029601No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT1167370.10320649258901052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT270950.048.4465561
CACGCGT353350.045.4342081
ACGCGTC373100.043.6281052
CGCGTGT1539750.039.1440661
GCGTGTT1593350.036.6934932
CGGCTTA229650.035.9261632
CCCCGCT661950.035.8408051
CCGGCTT255700.034.1805761
CCCGCTC816650.032.640682
CGCTCTA824250.032.531534
CGCGTCT635900.031.443883
CGGTACT231650.031.3510912
GCGGATT188000.031.0939331
GGCATTC399550.031.0156152
GCGGTAC251400.030.228151
CCGCTCT907400.030.1014563
CGTGTTT1990300.029.3657633
CGAAACC100100.029.182232
CCGAAAC103000.029.1613921
TCCCACG541700.028.695859
Produced by FastQC (version 0.11.5)