##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113110132 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.019829054748165 39.0 37.0 39.0 33.0 39.0 2 36.63856331632607 39.0 36.0 39.0 32.0 39.0 3 36.60995165313749 39.0 36.0 39.0 32.0 39.0 4 36.54248243649826 39.0 36.0 39.0 32.0 39.0 5 36.507704349597965 39.0 36.0 39.0 32.0 39.0 6 36.56685204823207 39.0 36.0 39.0 32.0 39.0 7 36.58556551768501 39.0 36.0 39.0 32.0 39.0 8 36.518219039829255 39.0 36.0 39.0 32.0 39.0 9 36.47534710683566 39.0 36.0 39.0 31.0 39.0 10-11 36.46359587397529 39.0 36.0 39.0 31.5 39.0 12-13 36.37302530510706 39.0 36.0 39.0 31.0 39.0 14-15 36.28096052438521 39.0 36.0 39.0 31.0 39.0 16-17 36.14505635534047 39.0 36.0 39.0 31.0 39.0 18-19 36.01723180289454 38.0 36.0 39.0 31.0 39.0 20-21 35.85808916304686 38.0 36.0 39.0 30.0 39.0 22-23 35.68565214829738 38.0 35.5 39.0 30.0 39.0 24-25 35.51232809983813 38.0 35.0 39.0 29.0 39.0 26-27 35.27368272808664 38.0 35.0 39.0 28.5 39.0 28-29 35.02742895304905 37.5 35.0 39.0 28.0 39.0 30-31 34.85887186039178 37.5 35.0 39.0 27.0 39.0 32-33 34.760267594772145 37.0 34.5 39.0 27.0 39.0 34-35 34.60835961627204 37.0 34.0 39.0 27.0 39.0 36-37 34.94404554315258 38.0 35.0 39.0 28.0 39.0 38-39 34.77576272742746 37.0 35.0 39.0 27.5 39.0 40-41 34.749329105194576 37.5 34.5 39.0 27.5 39.0 42-43 34.6420356003121 37.0 34.5 39.0 27.0 39.0 44-45 34.444834628077345 37.0 34.0 39.0 27.0 39.0 46-47 34.091243921455245 37.0 34.0 39.0 26.0 39.0 48-49 33.847549293815696 37.0 33.0 39.0 25.5 39.0 50-51 33.95490305855182 37.0 33.5 39.0 25.0 39.0 52-53 34.03042543969447 37.0 34.0 39.0 25.0 39.0 54-55 33.89688401212369 37.0 34.0 39.0 25.0 39.0 56-57 34.232263595979184 37.5 34.5 39.0 25.5 39.0 58-59 34.026228543345695 37.0 34.0 39.0 25.0 39.0 60-61 33.862347919459594 37.0 34.0 39.0 24.5 39.0 62-63 33.76660598362665 37.0 34.0 39.0 24.0 39.0 64-65 33.53508631304577 37.0 33.0 39.0 23.5 39.0 66-67 33.434254090517726 37.0 33.5 39.0 23.0 39.0 68-69 33.16323319735848 37.0 33.0 39.0 22.0 39.0 70-71 32.9105236743955 37.0 33.0 39.0 21.0 39.0 72-73 32.55764661736934 36.5 32.5 39.0 20.0 39.0 74-75 32.209983138380565 36.0 32.0 39.0 18.5 39.0 76-77 31.76887178418287 36.0 31.5 38.5 14.0 39.0 78-79 31.345694871083694 36.0 30.5 38.0 12.0 39.0 80-81 30.828335740957318 35.5 30.0 37.0 4.0 39.0 82-83 30.21721420588564 35.0 29.0 37.0 2.0 39.0 84-85 29.68744037890434 34.5 28.0 37.0 2.0 39.0 86-87 28.895647964587294 34.0 27.0 37.0 2.0 39.0 88-89 28.24435454199629 33.5 25.5 36.5 2.0 39.0 90-91 27.297036652737702 33.0 23.5 36.0 2.0 38.5 92-93 26.199038888929948 32.5 20.5 36.0 2.0 37.5 94-95 25.198890476053904 32.0 15.5 36.0 2.0 37.0 96-97 23.650186236189697 31.0 2.0 35.5 2.0 37.0 98-99 22.49726568261807 29.5 2.0 35.0 2.0 37.0 100-101 21.641473347409764 28.5 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 0.3451631158678907 1 2 0.37051254177351467 1 3 0.4342473733303365 1 4 0.462010159273774 1 5 0.42244969820065137 1 6 0.44213943702563085 1 7 0.2898642321647955 1 8 0.4176151433724584 1 9 0.361900661752081 1 10-11 0.3590836673192328 1 12-13 0.37829786764697815 1 14-15 0.36294899519429435 1 16-17 0.28700155467454636 1 18-19 0.2135979951507494 1 20-21 0.14321319484720618 1 22-23 0.006814186137589218 1 24-25 0.013682353646395029 1 26-27 -0.16553049041144874 1 28-29 -0.31827774243985374 1 30-31 -0.7098684281428618 1 32-33 -0.4755678979439679 1 34-35 -0.4274461469530806 1 36-37 -0.30951000373050164 1 38-39 -0.3649564324809802 1 40-41 -0.2653034402562753 1 42-43 -0.0976459693301237 1 44-45 -0.15918013567048916 1 46-47 -0.05640601432180148 1 48-49 -0.2964020421799347 1 50-51 -0.09377466852247096 1 52-53 -0.06933515700636406 1 54-55 -0.3961158817989485 1 56-57 -0.3184418604337864 1 58-59 -0.3552227284885987 1 60-61 -0.26557130346885316 1 62-63 -0.295472948528932 1 64-65 -0.3189131474037836 1 66-67 -0.19732302422431758 1 68-69 -0.20610666613909956 1 70-71 -0.25585897099193744 1 72-73 -0.22743016000384841 1 74-75 -0.22702478098088008 1 76-77 -0.28756250177714193 1 78-79 -0.5169597630602993 1 80-81 -0.4924123081367533 1 82-83 -0.6270363008843844 1 84-85 -0.7499073793413977 1 86-87 -0.9197998313571709 1 88-89 -1.0344604925423155 1 90-91 -1.1222774427017868 1 92-93 -1.3654919187711876 1 94-95 -1.5767280877276733 1 96-97 -1.4910487162490043 1 98-99 -1.5271291054028744 1 100-101 -1.5225914122649584 2 1 0.2333530364350267 2 2 0.3189418874411416 2 3 0.28058079537096603 2 4 0.27377451666261265 2 5 0.30469308392533634 2 6 0.3269041203913403 2 7 0.3017034561127616 2 8 0.31418745900962364 2 9 0.3554328146430308 2 10-11 0.24452678133798855 2 12-13 0.22165995250841775 2 14-15 0.2353014791868091 2 16-17 0.2641480352617265 2 18-19 0.2799922262201733 2 20-21 0.3250690946814814 2 22-23 0.3348377919584209 2 24-25 0.37334922035591234 2 26-27 0.48366680505832704 2 28-29 0.5258575754133048 2 30-31 0.46173229119548864 2 32-33 0.43191564473301014 2 34-35 0.4467914539874158 2 36-37 0.34559987169017603 2 38-39 0.3263042421093587 2 40-41 0.28282224379587717 2 42-43 0.2338390313087686 2 44-45 0.26146526363123 2 46-47 0.16529054044207214 2 48-49 0.20775301409732094 2 50-51 0.2525855741318068 2 52-53 0.3337932684517284 2 54-55 0.31615425199307623 2 56-57 0.3062251682447936 2 58-59 0.25669441161785755 2 60-61 0.269984362809474 2 62-63 0.19672519606390182 2 64-65 0.2113571838337336 2 66-67 0.0680918160228785 2 68-69 0.011211267888093346 2 70-71 0.0537630784540184 2 72-73 -0.02755752304393866 2 74-75 0.05135332059387565 2 76-77 0.13345193817844958 2 78-79 0.1251029465386111 2 80-81 0.15016640608356724 2 82-83 0.06694422481546525 2 84-85 -0.049177715743280004 2 86-87 -0.19716076058963594 2 88-89 -0.22009537197987328 2 90-91 -0.2607802439238682 2 92-93 -0.38229205874572614 2 94-95 -0.4373766121871441 2 96-97 -0.3217631613184082 2 98-99 -0.32412362051611865 2 100-101 -0.33267128817075076 3 1 0.011911927381554221 3 2 0.13030211236306855 3 3 0.08053389584036807 3 4 -0.1133207261160365 3 5 0.03792203888919232 3 6 -0.013240736894445604 3 7 -0.1965702004329941 3 8 0.051779058765980324 3 9 0.008985540445742402 3 10-11 0.10416480309728371 3 12-13 0.25107841825242616 3 14-15 0.22023074626056172 3 16-17 0.300296241191397 3 18-19 0.35554914740834676 3 20-21 0.3948920456874774 3 22-23 0.38382234624803857 3 24-25 0.43807589646765877 3 26-27 0.5223803550836621 3 28-29 0.5388953650969128 3 30-31 0.5215187883119725 3 32-33 0.497061219177219 3 34-35 0.4962292037907545 3 36-37 0.4274188802615768 3 38-39 0.3686432987631605 3 40-41 0.33904129909106473 3 42-43 0.3710205522141692 3 44-45 0.338474771906057 3 46-47 0.3354977651246145 3 48-49 0.3636552697459621 3 50-51 0.33828653337821635 3 52-53 0.407463104976145 3 54-55 0.4386006830134832 3 56-57 0.4651084411525517 3 58-59 0.4504298320122899 3 60-61 0.4343392368066148 3 62-63 0.3782520627480537 3 64-65 0.426829235960291 3 66-67 0.44844991210289464 3 68-69 0.3728562277467802 3 70-71 0.34034638209836743 3 72-73 0.296559264613272 3 74-75 0.38478638353616645 3 76-77 0.39745764934007255 3 78-79 0.4381758277392791 3 80-81 0.3937206063606489 3 82-83 0.3443801633140211 3 84-85 0.40406615869713747 3 86-87 0.5324914183965603 3 88-89 0.5418442641226555 3 90-91 0.5921998374089839 3 92-93 0.6169343742066786 3 94-95 0.6538820797042817 3 96-97 0.6644916398891993 3 98-99 0.5363560592371464 3 100-101 0.4674870936544586 4 1 0.1569858294741664 4 2 0.207303204640489 4 3 0.17015945919460052 4 4 0.15461697301842747 4 5 0.19869426252702027 4 6 0.1397875874231005 4 7 0.1553801087653497 4 8 0.1542903303906371 4 9 0.13731139538506199 4 10-11 0.1275045526920522 4 12-13 0.12288228680366586 4 14-15 0.17335793961713364 4 16-17 0.0514082424613207 4 18-19 0.1731228080723426 4 20-21 0.17369195046995856 4 22-23 0.20736737761335178 4 24-25 0.2582601268384721 4 26-27 0.3170142777331719 4 28-29 0.39311395137860217 4 30-31 0.32732497123959803 4 32-33 0.34778235193686413 4 34-35 0.31747132974032155 4 36-37 0.2030002966452642 4 38-39 0.26990600748640503 4 40-41 0.21348406474580628 4 42-43 0.1850829202986617 4 44-45 0.23918366484191722 4 46-47 0.19936503065503075 4 48-49 0.2548031629586518 4 50-51 0.22300656320337708 4 52-53 0.2870751509804421 4 54-55 0.2678813767115926 4 56-57 0.2510352162239755 4 58-59 0.23444308305013806 4 60-61 0.32760506251181454 4 62-63 0.2683670203556545 4 64-65 0.31068084965819764 4 66-67 0.3099309718349659 4 68-69 0.33866454763842313 4 70-71 0.3880297919297604 4 72-73 0.44069178025230826 4 74-75 0.4626390670843108 4 76-77 0.5289315092242113 4 78-79 0.4860636685883222 4 80-81 0.5910423316644504 4 82-83 0.6163799454237306 4 84-85 0.6477038533218433 4 86-87 0.7281876478480349 4 88-89 0.9146864095998026 4 90-91 1.0565242807570705 4 92-93 1.1198246438021862 4 94-95 1.0901992807257521 4 96-97 1.1108156656450134 4 98-99 1.1285628754743158 4 100-101 1.1275407745620427 5 1 0.011748316481110521 5 2 0.04179141253388963 5 3 0.008206397499876061 5 4 0.04106196570350562 5 5 0.03193700098124452 5 6 0.050584169966512604 5 7 0.023128945557736813 5 8 0.005835739500199111 5 9 0.032331459632409576 5 10-11 -0.031013327225743126 5 12-13 -0.0014914012207611904 5 14-15 -6.101071792912194E-4 5 16-17 -0.528118595639107 5 18-19 -0.1357468699360922 5 20-21 -0.20255863822616504 5 22-23 -0.11466263939927046 5 24-25 0.008982355454278945 5 26-27 -0.2783211067503828 5 28-29 -0.4010093130999337 5 30-31 -0.5998712749023767 5 32-33 -0.23142676573015564 5 34-35 -0.5244670981848074 5 36-37 -0.40495652488760214 5 38-39 -0.23933220721042403 5 40-41 -0.3138484826537109 5 42-43 -0.15406656928988127 5 44-45 -0.1943887733632934 5 46-47 -0.06007708512253629 5 48-49 -0.018700029389421502 5 50-51 0.003460602334939722 5 52-53 0.08482867373051306 5 54-55 0.10607603598320026 5 56-57 0.19880469575511484 5 58-59 0.173077381201459 5 60-61 0.2255491515987771 5 62-63 0.26160374752002014 5 64-65 0.2622510888915386 5 66-67 0.2757743681926428 5 68-69 0.32226653938263183 5 70-71 0.3515585958598848 5 72-73 0.45627626416792566 5 74-75 0.47063456902694867 5 76-77 0.5971464691329835 5 78-79 0.6385306955884928 5 80-81 0.7147426616624024 5 82-83 0.8283106426073701 5 84-85 0.9154450612896348 5 86-87 1.1047067802374038 5 88-89 1.2374099586211358 5 90-91 1.3959739493011796 5 92-93 1.435940819843033 5 94-95 1.5275124081724734 5 96-97 1.5919767212174634 5 98-99 1.5923117747825195 5 100-101 1.583386506752582 6 1 -0.06812704535470004 6 2 -0.090338339684223 6 3 -0.26895862486754396 6 4 -0.16085712874089353 6 5 -0.12187182226308124 6 6 -0.09113972657112868 6 7 -0.16399473198599424 6 8 -0.1386748762834884 6 9 -0.1383069524188869 6 10-11 -0.15903985497852346 6 12-13 -0.1494663045578335 6 14-15 -0.14210793987117398 6 16-17 -0.09227025512717546 6 18-19 -0.12230014197989902 6 20-21 -0.03691438424088034 6 22-23 -0.046247245062005504 6 24-25 0.0165415423655233 6 26-27 0.19743682222624415 6 28-29 0.2011422160819123 6 30-31 0.2177798318695423 6 32-33 0.10602904336112573 6 34-35 0.04538397688173035 6 36-37 3.047140357068656E-4 6 38-39 0.011482942020975884 6 40-41 -0.005588442692854301 6 42-43 -0.028963861642729682 6 44-45 3.662228038834314E-4 6 46-47 -0.011033727395670212 6 48-49 -0.05188115952242356 6 50-51 -0.04270317579219096 6 52-53 0.020081196315992145 6 54-55 0.05395514137659774 6 56-57 0.13012157906082678 6 58-59 0.15798107658632432 6 60-61 0.11627299601953922 6 62-63 0.1762757923304079 6 64-65 0.17380300956543948 6 66-67 0.06295416413320254 6 68-69 0.13351821320046753 6 70-71 0.14201319246104305 6 72-73 0.22951746789074434 6 74-75 0.21914134094313908 6 76-77 0.46858225640479745 6 78-79 0.49762759528633893 6 80-81 0.5807112795201057 6 82-83 0.8244387982536132 6 84-85 0.9003675738606844 6 86-87 1.1429359634088634 6 88-89 1.2409304140774964 6 90-91 1.4147876702762048 6 92-93 1.6358775323393537 6 94-95 1.6909483081586565 6 96-97 1.8833835465215323 6 98-99 1.961429242243529 6 100-101 2.0272367333390093 7 1 -0.1044434006111743 7 2 -0.1566152809743926 7 3 -0.1801609165239313 7 4 -0.18518483823619647 7 5 -0.22701344776216104 7 6 -0.20930726279277678 7 7 -0.2143775081894006 7 8 -0.29586092528990804 7 9 -0.21127056373251207 7 10-11 -0.21791522966432098 7 12-13 -0.2450318656037993 7 14-15 -0.33087890179289303 7 16-17 -0.18538901040415112 7 18-19 -0.17345666379375047 7 20-21 -0.12392827353903613 7 22-23 -0.13572418217523818 7 24-25 -0.035813729456684484 7 26-27 0.08990076241608591 7 28-29 0.18665854623368716 7 30-31 0.22725500957191258 7 32-33 0.18216989194916522 7 34-35 0.15790128543599025 7 36-37 0.022185757584125554 7 38-39 -0.00257165354013722 7 40-41 -0.02510462991197926 7 42-43 -0.06218505173051625 7 44-45 -0.00985226222206137 7 46-47 0.015726120198891635 7 48-49 0.07136129507875211 7 50-51 0.0062818732273299815 7 52-53 0.02217111241418479 7 54-55 0.06693140744143733 7 56-57 0.06124727810127695 7 58-59 0.14464913317543449 7 60-61 0.21502887216372812 7 62-63 0.28582885427065463 7 64-65 0.3334514626265559 7 66-67 0.38556985989279724 7 68-69 0.3662963272754922 7 70-71 0.44240016210657274 7 72-73 0.43524136056740304 7 74-75 0.5981712127089054 7 76-77 0.7346625701735725 7 78-79 0.8292672164960635 7 80-81 0.9921455165221005 7 82-83 1.1006558412397922 7 84-85 1.2833349495985154 7 86-87 1.6242571674246413 7 88-89 1.840124565987331 7 90-91 2.0713902303647913 7 92-93 2.2759601206508115 7 94-95 2.4080692058182827 7 96-97 2.6586821460383447 7 98-99 2.8254711405900785 7 100-101 2.8382949837871863 8 1 -0.5845331172852752 8 2 -0.9193114215111251 8 3 -0.6325672874645392 8 4 -0.6724976289329732 8 5 -0.6608824364767045 8 6 -0.6681425292386436 8 7 -0.6831819710830516 8 8 -0.9560521251474867 8 9 -0.6283492037660849 8 10-11 -0.7381989366364721 8 12-13 -0.9821222453917358 8 14-15 -0.761931917162876 8 16-17 -0.4255097678371058 8 18-19 -0.591404956827013 8 20-21 -0.33462018128412296 8 22-23 -0.4126701034282121 8 24-25 -0.3543826805635888 8 26-27 -0.6486508766586851 8 28-29 -0.2967590991591038 8 30-31 -0.09764898148986134 8 32-33 -0.15188218886168414 8 34-35 -0.07207086546531372 8 36-37 -0.27134837330675765 8 38-39 -0.29229931446584345 8 40-41 -0.39983092906732054 8 42-43 -0.35549739104758515 8 44-45 -0.408508040058301 8 46-47 -0.4741346309511485 8 48-49 -0.35992288849884346 8 50-51 -0.36647833643626626 8 52-53 -1.0308144848737726 8 54-55 -0.6343170846070834 8 56-57 -0.3598926569775358 8 58-59 -0.47158665324609217 8 60-61 -0.2993380321946688 8 62-63 -0.07801114086830552 8 64-65 0.07880515767235607 8 66-67 0.18615073921037606 8 68-69 0.43092138176259454 8 70-71 0.5435238717340667 8 72-73 0.4679522821367712 8 74-75 0.5475078422003179 8 76-77 0.9307798015079278 8 78-79 1.0759657817432284 8 80-81 1.4679234983557343 8 82-83 1.6996519900590314 8 84-85 1.915205965466516 8 86-87 2.1279022384900124 8 88-89 2.15562884763289 8 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-0.6398466210664608 65 34-35 -0.5413327467198457 65 36-37 -0.18510365050923383 65 38-39 -0.4472347705139157 65 40-41 -0.04375490862383913 65 42-43 0.03730970182112259 65 44-45 0.035065359899626 65 46-47 0.0598347122243581 65 48-49 0.08616150206361795 65 50-51 0.005730232354963505 65 52-53 -8.948919743119177E-4 65 54-55 -0.1896680256323222 65 56-57 -0.21185921605550107 65 58-59 -0.0601122814395012 65 60-61 -0.028795894166080416 65 62-63 -0.05151284865223005 65 64-65 0.012265752811210007 65 66-67 0.06382999763187058 65 68-69 0.06699213160786854 65 70-71 0.08590605261088768 65 72-73 0.05797402998944534 65 74-75 0.05444081806180634 65 76-77 -0.057866749378643334 65 78-79 -0.040403778717504224 65 80-81 -0.03470144868824221 65 82-83 -0.16333397784150705 65 84-85 -0.11398610670897114 65 86-87 -0.07684099886533247 65 88-89 -0.09252799297493652 65 90-91 -0.04320519379731991 65 92-93 -0.08374240192777194 65 94-95 -0.147376560606709 65 96-97 -0.15542550716950387 65 98-99 -0.16653430672935698 65 100-101 -0.23431014095979208 66 1 -0.2357341479599384 66 2 -0.3420316988630958 66 3 -0.29940809792469736 66 4 -0.3077047397951631 66 5 -0.32108926686172623 66 6 -0.525863144868346 66 7 -0.23476961016156395 66 8 -0.2708383128244023 66 9 -0.6815245545238113 66 10-11 -0.22983018061452753 66 12-13 -0.31846954368754865 66 14-15 -0.2947990039849131 66 16-17 -0.13562171444645799 66 18-19 -0.1781037458682846 66 20-21 -0.12537031405250332 66 22-23 -0.05581663235555823 66 24-25 -0.023589952944639947 66 26-27 0.1300691899255284 66 28-29 0.17562266379015767 66 30-31 0.18693973843378586 66 32-33 0.06574921131267075 66 34-35 0.08957055770189726 66 36-37 0.05733969207775402 66 38-39 0.04019567315133088 66 40-41 -0.008195374006078282 66 42-43 -0.0069344367304822185 66 44-45 -0.04120173831787355 66 46-47 -0.12790831194142527 66 48-49 -0.09413897805661264 66 50-51 -0.031181623561600702 66 52-53 0.03387537226092263 66 54-55 0.07522243422057073 66 56-57 0.05222742009161152 66 58-59 0.05812054853243609 66 60-61 0.002529015737472662 66 62-63 -0.037539003560198125 66 64-65 -0.0020913061388725396 66 66-67 -0.13534610125537938 66 68-69 -0.03680188041110455 66 70-71 -0.14068865643880457 66 72-73 -0.1664232142710631 66 74-75 -0.1010948307253372 66 76-77 -0.0683195301129409 66 78-79 -0.03164772339522415 66 80-81 0.006296720164268521 66 82-83 0.06283601813834849 66 84-85 0.15206226459509864 66 86-87 0.19333774917232915 66 88-89 0.21323053080599763 66 90-91 0.20464075093523704 66 92-93 0.19588288454335157 66 94-95 0.33381090914811296 66 96-97 0.40075567276225144 66 98-99 0.4033809751391111 66 100-101 0.4014831237117029 67 1 -0.09272272990418884 67 2 -0.17998147291839928 67 3 -0.3808541939743364 67 4 -0.19902605375914106 67 5 -0.1967739477889907 67 6 -0.2474060378587737 67 7 -0.25190357790755513 67 8 -0.18981214466913343 67 9 -0.19260285615484918 67 10-11 -0.2412967168835749 67 12-13 -0.22756047616449138 67 14-15 -0.2695490177147306 67 16-17 -0.21955185054107318 67 18-19 -0.3145579093549742 67 20-21 -0.36924508614266927 67 22-23 -0.3520438530033161 67 24-25 -0.33051129333086493 67 26-27 -0.15413146285043666 67 28-29 -0.16649216964611213 67 30-31 -0.02482267683766537 67 32-33 -0.05749503919001597 67 34-35 -0.07932771959358575 67 36-37 -0.1594925337750439 67 38-39 -0.26274843974967155 67 40-41 -0.2057489389201521 67 42-43 -0.17423956364373083 67 44-45 -0.14003690442739725 67 46-47 -0.19364532970683968 67 48-49 -0.12930205787333904 67 50-51 -0.11724796754216982 67 52-53 -0.045074813001065195 67 54-55 -0.021478374321397098 67 56-57 0.03338186064480908 67 58-59 -0.036381236830507646 67 60-61 -0.011143370773005756 67 62-63 0.055095461822070035 67 64-65 0.07835894923078257 67 66-67 0.13809753743127118 67 68-69 0.13819581239779666 67 70-71 0.18866385203323688 67 72-73 0.2842988817637462 67 74-75 0.28303508309032566 67 76-77 0.12901489764236374 67 78-79 0.3542994058737783 67 80-81 0.5299161242539974 67 82-83 0.5881024218365383 67 84-85 0.7472572208134949 67 86-87 1.0055853275690474 67 88-89 1.1209632305521815 67 90-91 1.2628636819669907 67 92-93 1.324798067345796 67 94-95 1.3760567400202817 67 96-97 1.3543645865583542 67 98-99 1.4989076646902326 67 100-101 1.4160318218603436 68 1 -0.6548037755688156 68 2 -0.7495413749319084 68 3 -0.7383252754406442 68 4 -0.7703881597492526 68 5 -0.6904943758579165 68 6 -0.9628349860119485 68 7 -0.7376690350268902 68 8 -0.7650322183548468 68 9 -0.6967692822332765 68 10-11 -0.796124000874741 68 12-13 -0.744651043368016 68 14-15 -0.6803598259297345 68 16-17 -0.6918747103610343 68 18-19 -0.68211779780939 68 20-21 -0.5277435047554917 68 22-23 -0.5104931726218283 68 24-25 -0.4887575928277741 68 26-27 -0.5080154925997036 68 28-29 -0.5935835652403867 68 30-31 -0.2594377135953252 68 32-33 -0.3279661054425915 68 34-35 -0.37025342549341644 68 36-37 -0.2709736219593495 68 38-39 -0.3495005323868554 68 40-41 -0.3766452637398032 68 42-43 -0.3788357368196742 68 44-45 -0.3228347240994438 68 46-47 -0.2743138350958816 68 48-49 -0.36123451931045736 68 50-51 -0.4067351640543464 68 52-53 -0.6556516410153392 68 54-55 -0.5318850296028117 68 56-57 -0.6046097063011331 68 58-59 -0.4724457282488146 68 60-61 -0.5742012117458373 68 62-63 -0.3375222982123134 68 64-65 -0.39709029194306567 68 66-67 -0.2480969349207527 68 68-69 -0.18172528302354607 68 70-71 -0.06759218235355746 68 72-73 -0.042247577671702174 68 74-75 -0.0038037184774424304 68 76-77 -0.16023313235133685 68 78-79 0.1172552527765447 68 80-81 0.11554356099091834 68 82-83 0.4998002009103999 68 84-85 0.7534052273475531 68 86-87 1.1300687541241352 68 88-89 1.2081893836856565 68 90-91 1.1432033351495718 68 92-93 1.2725626446323517 68 94-95 1.8565568006585558 68 96-97 1.9564036687645405 68 98-99 1.2342888027616752 68 100-101 1.606560617766874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 734243.0 3 102645.0 4 154664.0 5 182661.0 6 232982.0 7 255978.0 8 335143.0 9 331327.0 10 425665.0 11 417916.0 12 480438.0 13 385559.0 14 425754.0 15 428277.0 16 471491.0 17 530616.0 18 582795.0 19 676769.0 20 779360.0 21 919646.0 22 1046858.0 23 1214759.0 24 1375925.0 25 1582912.0 26 1827762.0 27 2115364.0 28 2482764.0 29 2925952.0 30 3506288.0 31 4277609.0 32 5327451.0 33 6747603.0 34 8616802.0 35 1.1155054E7 36 1.4966759E7 37 2.056189E7 38 1.4524355E7 39 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.23143848228295 11.334491557611496 13.391498194088705 42.04257176601685 2 21.176133211519087 18.42175683050216 37.63487526212313 22.767234695855624 3 25.734693083112038 19.160634273283254 26.078707216150494 29.025965427454214 4 28.681075359940138 23.54172936680644 25.14820221932809 22.62899305392533 5 24.17490994127935 25.927864730788126 31.10408470070564 18.793140627226883 6 20.814941379276224 29.662988483666027 28.615388699495647 20.906681437562106 7 15.958410167179244 15.578119486561606 47.347307261167884 21.116163085091262 8 19.888514299705292 21.732072050531894 33.97758898787841 24.401824661884405 9 19.417657146351214 20.763133185630345 35.31537060912738 24.503839058891067 10-11 22.83729157422776 28.521964276156965 26.111072643940663 22.529671505674617 12-13 20.406597650108555 22.746602103432455 33.30222826947444 23.544571976984546 14-15 20.750476819848533 24.211001728583867 32.5589204682081 22.4796009833595 16-17 21.299366352766544 24.318643129830004 31.108982339235304 23.27300817816815 18-19 21.33162154395182 24.695165352364683 31.095690040947588 22.87752306273591 20-21 21.378236783853865 24.962936853935627 30.529616656537762 23.129209705672743 22-23 20.981777797768643 24.75527367452363 31.298257836563693 22.964690691144032 24-25 21.12273463128494 24.52445716171964 31.374073788611422 22.978734418383997 26-27 20.720105066449417 24.804553957257227 30.80344994076567 23.671891035527683 28-29 21.08916108398078 25.528921780177587 30.111677799276332 23.2702393365653 30-31 20.997283304201154 25.17032918609752 30.38336995375998 23.449017555941342 32-33 21.360088108873658 25.56376315030413 29.854934083041755 23.22121465778045 34-35 22.17674421229082 26.175425240680084 28.613433747434964 23.034396799594134 36-37 23.07779497215887 26.41619304161395 28.474841483892604 22.031170502334575 38-39 23.505346739478995 27.389561739904956 27.681028357352684 21.42406316326337 40-41 24.767751532963885 27.47156883664711 26.606314302325462 21.154365328063545 42-43 25.366151431978786 27.611711418392876 25.777047985729396 21.245089163898946 44-45 26.736288175114648 27.434747997106935 25.215100062941403 20.613863764837017 46-47 27.284921791079135 27.415880980307506 24.901892949924235 20.397304278689123 48-49 28.6007541086894 26.505042282300533 24.55976730413 20.334436304880068 50-51 29.529385572638176 26.01211578464076 24.72664075752294 19.731857885198117 52-53 30.76778877775512 26.063927677142136 24.108729269275365 19.059554275827388 54-55 31.674282221301688 26.765835507954133 23.568843992229862 17.991038278514317 56-57 32.605667129030365 27.135421874108022 23.146865081585506 17.11204591527611 58-59 33.842121612738026 27.391895720824248 22.429951147825435 16.336031518612295 60-61 34.23673783468659 28.491448073847074 21.921847562401915 15.349966529064424 62-63 34.395228819785515 28.90857907002438 22.119844759788837 14.576347350401258 64-65 34.80874551538849 28.863225091099707 22.302565255604158 14.025464137907646 66-67 34.91426888719216 28.680361558860927 22.533978684468178 13.871390869478741 68-69 35.02367203172719 28.32323327412522 22.823895430064432 13.829199264083162 70-71 35.40859066089996 27.827776648809277 23.088807774384623 13.67482491590614 72-73 35.459228096505825 27.115704402617524 23.478234612118353 13.946832888758298 74-75 35.83624230591734 25.902622607842083 23.711336336956958 14.54979874928362 76-77 36.66060119685487 24.588056462012716 23.50909069584346 15.24225164528895 78-79 36.71896124191338 23.631537760651266 23.956604750579316 15.692896246856039 80-81 36.67160675800674 22.74208988874802 24.160579270763108 16.42572408248214 82-83 36.05154422462198 22.02975132824848 24.586909138111956 17.331795309017583 84-85 34.94000584536336 21.857758760503916 25.158577774082996 18.04365762004973 86-87 34.04837069197049 22.364860595453454 25.331767309563585 18.25500140301247 88-89 32.56783785656598 23.680745494842494 25.784197879867154 17.967218768724372 90-91 30.941621430763895 25.489827913975294 25.764827648384315 17.803723006876503 92-93 29.407638211928187 27.680798636706132 25.609505129041 17.302058022324676 94-95 27.857803302571725 30.523308557396195 24.891356373271357 16.727531766760716 96-97 26.448961979306524 33.45390040536254 23.94905131660319 16.148086298727748 98-99 24.9552848571992 36.30975259256047 23.131253908926222 15.603708641314112 100-101 23.58454455544187 39.097126492387915 22.080098745330996 15.238230206839221 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1174.0 1 885.0 2 582.5 3 741.5 4 922.5 5 1573.0 6 2333.0 7 2580.5 8 2964.5 9 3466.5 10 4070.0 11 4700.5 12 5432.5 13 6380.0 14 7751.0 15 9631.5 16 11650.5 17 14636.0 18 19801.0 19 24333.0 20 28347.5 21 33509.0 22 39084.5 23 45992.5 24 55078.5 25 66751.5 26 80735.5 27 100483.5 28 125338.0 29 158368.0 30 203223.5 31 259109.0 32 346790.0 33 462080.5 34 603131.0 35 830263.5 36 1045795.0 37 1240008.0 38 1523750.5 39 1846103.5 40 2317777.0 41 3016747.0 42 3800537.0 43 4718357.0 44 5743610.0 45 6705822.0 46 7538738.0 47 8123437.0 48 8304601.5 49 8032954.0 50 7482034.5 51 6840037.0 52 6165092.5 53 5472797.5 54 4703876.0 55 3871966.5 56 3089181.5 57 2388924.5 58 1769449.0 59 1256511.0 60 849868.0 61 546483.5 62 357290.5 63 242988.5 64 165327.5 65 110370.0 66 74848.0 67 52583.0 68 37172.0 69 26189.0 70 19153.5 71 14425.0 72 10977.0 73 8924.0 74 7337.5 75 5521.0 76 4272.5 77 3284.5 78 2560.5 79 2043.0 80 1616.5 81 1320.0 82 1075.5 83 854.5 84 609.0 85 404.0 86 271.5 87 176.0 88 129.0 89 109.5 90 92.5 91 69.5 92 52.5 93 47.5 94 39.0 95 28.5 96 27.0 97 23.5 98 22.5 99 35.0 100 58.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05819107345750423 2 0.038894835698715303 3 0.024887248827540932 4 0.010872589203591417 5 0.028015173742348742 6 0.023418768532601483 7 0.0026248753736756317 8 6.524614435071121E-4 9 0.018183163290800507 10-11 0.00444478307213009 12-13 0.015098558986740463 14-15 0.021148414891779987 16-17 0.04342316566300179 18-19 0.04582303908901812 20-21 0.04424493112606393 22-23 0.0025811127158794226 24-25 0.005206872183651947 26-27 0.03399474416668526 28-29 0.019598155892877927 30-31 0.019304636652709414 32-33 0.020934464120331855 34-35 0.0355609168593314 36-37 0.043867422946690576 38-39 0.042397616510605786 40-41 0.06192416078163537 42-43 0.048440399662870164 44-45 0.053520404343617954 46-47 0.055095417977233024 48-49 0.0342723497131097 50-51 0.0 52-53 0.0 54-55 8.38563250903111E-4 56-57 6.727956077356536E-4 58-59 7.404288061479762E-4 60-61 5.1277457619800144E-5 62-63 4.42047048446553E-6 64-65 0.0 66-67 2.5638728809900072E-5 68-69 6.10024926856243E-5 70-71 0.004784275205337043 72-73 0.07263319257730157 74-75 0.004591100645165899 76-77 0.009390405450150125 78-79 0.07294350960531104 80-81 0.06879047758515568 82-83 0.01080672419337288 84-85 0.012072746940123807 86-87 0.01570681572540292 88-89 0.01743300944958671 90-91 0.011754473065242289 92-93 0.010343016839552445 94-95 0.01175845148867831 96-97 0.011909189532198584 98-99 0.013804687275937403 100-101 0.013406844932335505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.13110132E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.854297495783452 #Duplication Level Percentage of deduplicated Percentage of total 1 51.022888091845864 9.10977823085883 2 17.810593845381423 6.359912821840194 3 10.476793495838747 5.611673636297824 4 5.627163377678198 4.018761960097769 5 3.360834595919921 3.000267035483772 6 2.0293490877750777 2.1739561401559806 7 1.399178248278363 1.7486941286073736 8 0.9670469277819272 1.3812754832801557 9 0.7711520077039712 1.2391539624015657 >10 5.340326850977667 18.80089317247925 >50 0.6364632787909589 7.955553125352592 >100 0.4805398150110883 16.952147847821482 >500 0.047716317318363885 5.844184008341874 >1k 0.026729732432942275 8.895707428566412 >5k 0.002075994590062309 2.5511932856194153 >10k+ 0.0011483326752827418 4.356847732795612 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGCCCATGGCTTCGTCCAGACAGCGCAGCTGCAGTATGGCTGGACAGA 153478 0.1356889938029601 No Hit GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 116737 0.10320649258901052 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.066832845708287E-5 8.840940968931059E-7 0.0 8.840940968931059E-7 0.0 2 7.5147998235914E-5 3.5363763875724237E-6 0.0 1.7681881937862119E-6 0.0 3 1.3084592634017968E-4 3.5363763875724237E-6 0.0 4.42047048446553E-6 0.0 4 1.8212338395997982E-4 3.5363763875724237E-6 0.0 8.84094096893106E-6 0.0 5 2.3163265338599375E-4 3.5363763875724237E-6 0.0 8.84094096893106E-6 8.840940968931059E-7 6 2.9263514607161804E-4 3.5363763875724237E-6 0.0 1.0609129162717271E-5 8.840940968931059E-7 7 3.5098535646656303E-4 3.5363763875724237E-6 0.0 1.6797787840969013E-5 8.840940968931059E-7 8 4.3055382518694256E-4 3.5363763875724237E-6 0.0 1.945007013164833E-5 8.840940968931059E-7 9 5.304564581358636E-4 3.5363763875724237E-6 0.0 2.0334164228541436E-5 8.840940968931059E-7 10-11 0.002603657115350197 3.5363763875724237E-6 0.0 2.5638728809900072E-5 1.326141145339659E-6 12-13 0.003025812046616655 3.5363763875724237E-6 1.326141145339659E-6 3.447966977883113E-5 2.210235242232765E-6 14-15 0.003882057179457628 3.5363763875724237E-6 3.0943293391258708E-6 4.464675189310185E-5 2.652282290679318E-6 16-17 0.004938991672293336 3.5363763875724237E-6 3.5363763875724237E-6 8.17787039626123E-5 2.652282290679318E-6 18-19 0.006267343052875228 3.5363763875724237E-6 3.5363763875724237E-6 1.7284039594260222E-4 3.5363763875724237E-6 20-21 0.05173453426789388 7.0727527751448475E-6 3.5363763875724237E-6 2.6920665250395074E-4 3.5363763875724237E-6 22-23 0.08376879977471868 7.0727527751448475E-6 3.5363763875724237E-6 3.0854883981569394E-4 3.5363763875724237E-6 24-25 0.17342036167016409 7.0727527751448475E-6 3.5363763875724237E-6 3.271148158504492E-4 3.5363763875724237E-6 26-27 0.552105270286485 7.0727527751448475E-6 3.5363763875724237E-6 3.42144415497632E-4 3.5363763875724237E-6 28-29 1.5521655478220113 7.0727527751448475E-6 3.5363763875724237E-6 3.5319559170879583E-4 3.5363763875724237E-6 30-31 3.1693495857647838 7.956846872037953E-6 3.5363763875724237E-6 3.682251913559786E-4 3.5363763875724237E-6 32-33 5.5674137132118275 7.956846872037953E-6 3.5363763875724237E-6 3.841388851000545E-4 3.5363763875724237E-6 34-35 8.60272800318189 8.398893920484506E-6 3.5363763875724237E-6 3.9386392016587865E-4 3.978423436018977E-6 36-37 12.520426110014618 8.84094096893106E-6 3.5363763875724237E-6 4.0535714342548906E-4 4.42047048446553E-6 38-39 16.759856667835912 8.84094096893106E-6 3.5363763875724237E-6 4.17292413733546E-4 4.42047048446553E-6 40-41 21.062686055392454 8.84094096893106E-6 3.5363763875724237E-6 4.217128842180115E-4 4.42047048446553E-6 42-43 25.34865267419191 8.84094096893106E-6 3.5363763875724237E-6 4.575186951421823E-4 4.42047048446553E-6 44-45 29.56727696153692 8.84094096893106E-6 3.5363763875724237E-6 4.805051416614031E-4 4.42047048446553E-6 46-47 33.788117672782846 8.84094096893106E-6 3.5363763875724237E-6 4.897881296787806E-4 4.42047048446553E-6 48-49 37.93077131233478 8.84094096893106E-6 3.5363763875724237E-6 4.986290706477117E-4 4.42047048446553E-6 50-51 41.852413362933746 8.84094096893106E-6 3.5363763875724237E-6 5.09238199810429E-4 4.42047048446553E-6 52-53 45.63173129353257 8.84094096893106E-6 3.5363763875724237E-6 5.136586702948946E-4 4.42047048446553E-6 54-55 49.26359691632223 8.84094096893106E-6 3.5363763875724237E-6 5.211734701184859E-4 4.42047048446553E-6 56-57 52.621868127605055 8.84094096893106E-6 3.5363763875724237E-6 5.295723640389705E-4 4.42047048446553E-6 58-59 55.708242388047076 8.84094096893106E-6 3.5363763875724237E-6 5.446019636861533E-4 4.42047048446553E-6 60-61 58.618202744206855 1.193527030805693E-5 3.5363763875724237E-6 5.58747469236443E-4 4.42047048446553E-6 62-63 61.31946561604225 1.3261411453396589E-5 3.5363763875724237E-6 5.622838456240154E-4 4.42047048446553E-6 64-65 63.850709678245266 1.7239834889415566E-5 3.5363763875724237E-6 5.680304572538205E-4 4.42047048446553E-6 66-67 66.26176998891665 2.2986446519220754E-5 3.5363763875724237E-6 5.888066685308085E-4 4.42047048446553E-6 68-69 68.51551769031619 2.961715224591905E-5 3.5363763875724237E-6 5.941112331121672E-4 4.42047048446553E-6 70-71 70.56937083231412 3.536376387572424E-5 3.5363763875724237E-6 5.967635154028465E-4 4.42047048446553E-6 72-73 72.47225474018543 4.5530845989994956E-5 3.5363763875724237E-6 5.967635154028465E-4 4.42047048446553E-6 74-75 74.22238177566621 5.1277457619800144E-5 3.5363763875724237E-6 6.007419388388655E-4 4.42047048446553E-6 76-77 75.86001137369374 5.967635154028465E-5 3.5363763875724237E-6 6.029521740810982E-4 4.42047048446553E-6 78-79 77.35881255978023 7.205366889678813E-5 3.5363763875724237E-6 6.042783152264379E-4 4.42047048446553E-6 80-81 78.71552346875521 8.664122149552438E-5 3.5363763875724237E-6 6.056044563717775E-4 4.42047048446553E-6 82-83 79.96257046185747 1.0211286819115374E-4 3.5363763875724237E-6 6.069305975171173E-4 4.42047048446553E-6 84-85 81.13607408750968 1.193527030805693E-4 3.5363763875724237E-6 6.188658678251742E-4 4.42047048446553E-6 86-87 82.19790425140694 1.2951978519484E-4 3.5363763875724237E-6 6.365477497630363E-4 4.42047048446553E-6 88-89 83.16716490084195 1.3703458501843142E-4 3.5363763875724237E-6 6.396420791021622E-4 4.42047048446553E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCATT 27095 0.0 48.446556 1 CACGCGT 35335 0.0 45.434208 1 ACGCGTC 37310 0.0 43.628105 2 CGCGTGT 153975 0.0 39.144066 1 GCGTGTT 159335 0.0 36.693493 2 CGGCTTA 22965 0.0 35.926163 2 CCCCGCT 66195 0.0 35.840805 1 CCGGCTT 25570 0.0 34.180576 1 CCCGCTC 81665 0.0 32.64068 2 CGCTCTA 82425 0.0 32.53153 4 CGCGTCT 63590 0.0 31.44388 3 CGGTACT 23165 0.0 31.351091 2 GCGGATT 18800 0.0 31.093933 1 GGCATTC 39955 0.0 31.015615 2 GCGGTAC 25140 0.0 30.22815 1 CCGCTCT 90740 0.0 30.101456 3 CGTGTTT 199030 0.0 29.365763 3 CGAAACC 10010 0.0 29.18223 2 CCGAAAC 10300 0.0 29.161392 1 TCCCACG 54170 0.0 28.69585 9 >>END_MODULE