FastQCFastQC Report
Fri 27 May 2016
SRR1233081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113110132
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCCATGGCTTCGTCCAGACAGCGCAGCTGCAGTATGGCTGGACAGA1218940.10776576584668825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT137300.033.1673742
CGGCTTA152500.032.7262232
GGCGGAT129350.031.2742251
CGGGGCT169450.030.8468151
CGAGCTA223600.029.94380812-13
CGGCGGT47850.029.1763521
GCGGATT212850.027.556861
GCGCGTC37650.027.1166782
GTTTATT654500.026.1864191
CGGGTGT111600.025.9588031
GCGGTAC193950.025.5007461
ACTCGTC182100.025.4316542-43
CCAAACG324600.025.1470537
CGGGGAT107150.025.080271
CGGGCTA31900.024.794921
CGGATTT216400.024.488942
CCCGAAA209350.024.4670051
TCGTCTA146000.024.23160744-45
GGCGAAT98050.024.2006511
CTGGAAT1196100.023.7144851
Produced by FastQC (version 0.11.5)