FastQCFastQC Report
Fri 27 May 2016
SRR1233080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110212931
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT5808600.5270343459062894No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA3247540.2946605239996748No Hit
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1712200.15535382141320603Illumina Single End PCR Primer 1 (100% over 31bp)
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA1665630.1511283644203238No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1629380.14783927668160826No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1569410.14239799139358703No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1443930.13101275747761396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT667400.082.023061
GGCATTC822800.064.3144452
GCATTCC1080900.051.2989043
ATTCCTG1168200.046.3758355
CTGCTGA1159250.044.9487729
TCCTGCT1196400.044.853167
TTCCTGC1213200.044.6008036
CCTGCTG1247100.041.9807558
TGAACCG545100.039.7051312-13
CGGGATT108550.039.688961
GAACCGA359600.038.53158614-15
CCAACGA151800.038.5052388
AACCGAG378700.036.61956414-15
CACGCGT373350.036.3758741
CAACGAA164500.035.8208479
ACGCGTC416450.034.234162
ACCGCTC165100.033.88103516-17
GAACCGC230450.033.58629614-15
CATTCCT1616150.033.1897964
TCGTCGC109450.031.83613886-87
Produced by FastQC (version 0.11.5)