##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233080_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110212931
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.0698442817023	39.0	37.0	39.0	33.0	39.0
2	36.710295028811096	39.0	37.0	39.0	32.0	39.0
3	36.68958713202174	39.0	37.0	39.0	32.0	39.0
4	36.630072745275235	39.0	37.0	39.0	32.0	39.0
5	36.589269620277136	39.0	37.0	39.0	32.0	39.0
6	36.66793554378842	39.0	37.0	39.0	33.0	39.0
7	36.638905792279495	39.0	37.0	39.0	33.0	39.0
8	36.59733442711908	39.0	37.0	39.0	32.0	39.0
9	36.54353702833654	39.0	36.0	39.0	32.0	39.0
10-11	36.51391957355712	39.0	36.5	39.0	32.0	39.0
12-13	36.404817543596586	39.0	36.0	39.0	31.5	39.0
14-15	36.34240520742525	39.0	36.0	39.0	31.0	39.0
16-17	36.2074475226505	39.0	36.0	39.0	31.0	39.0
18-19	36.08640538286746	39.0	36.0	39.0	31.0	39.0
20-21	35.92590025121463	39.0	36.0	39.0	30.5	39.0
22-23	35.741597127110246	38.0	36.0	39.0	30.0	39.0
24-25	35.57366416468862	38.0	35.5	39.0	29.5	39.0
26-27	35.42323652113018	38.0	35.5	39.0	29.0	39.0
28-29	35.20132658934549	38.0	35.0	39.0	28.5	39.0
30-31	35.025752794833124	38.0	35.0	39.0	28.0	39.0
32-33	34.947421396496566	38.0	35.0	39.0	28.0	39.0
34-35	34.81095148898635	37.0	35.0	39.0	27.0	39.0
36-37	35.158562092863676	38.0	35.0	39.0	28.5	39.0
38-39	35.00943530392092	38.0	35.0	39.0	28.0	39.0
40-41	34.95067854605917	37.5	35.0	39.0	28.0	39.0
42-43	34.85668597271948	37.0	35.0	39.0	28.0	39.0
44-45	34.69247529130679	37.0	34.5	39.0	27.5	39.0
46-47	34.328824346391805	37.0	34.0	39.0	27.0	39.0
48-49	34.08806672603598	37.0	34.0	39.0	26.0	39.0
50-51	34.18516757802222	37.5	34.0	39.0	26.0	39.0
52-53	34.29393692469716	37.5	34.5	39.0	26.0	39.0
54-55	34.154071521789035	37.0	34.0	39.0	25.5	39.0
56-57	34.47102250188773	38.0	35.0	39.0	26.5	39.0
58-59	34.281623133677485	37.5	34.5	39.0	26.0	39.0
60-61	34.129539037483724	37.0	34.0	39.0	25.5	39.0
62-63	34.029974087160426	37.0	34.0	39.0	25.0	39.0
64-65	33.80987362090933	37.0	34.0	39.0	24.5	39.0
66-67	33.70267179901059	37.0	34.0	39.0	24.0	39.0
68-69	33.410989972673896	37.0	33.0	39.0	23.0	39.0
70-71	33.143479720179116	37.0	33.0	39.0	22.0	39.0
72-73	32.76284014259633	37.0	33.0	39.0	20.5	39.0
74-75	32.376684007251384	36.0	32.0	39.0	19.5	39.0
76-77	31.928121977810388	36.0	31.5	38.5	15.5	39.0
78-79	31.425325350434605	36.0	31.0	38.0	12.0	39.0
80-81	30.847720799658255	35.5	30.0	37.0	2.0	39.0
82-83	30.172899584713882	35.0	29.0	37.0	2.0	39.0
84-85	29.534082956200486	34.5	28.0	37.0	2.0	39.0
86-87	28.213139531694335	34.0	25.5	37.0	2.0	39.0
88-89	26.983278876777174	33.0	21.5	36.0	2.0	39.0
90-91	26.562948303225873	33.0	21.5	36.0	2.0	38.0
92-93	25.494904295758182	32.0	18.0	36.0	2.0	37.5
94-95	24.533941629771192	31.0	7.5	36.0	2.0	37.0
96-97	23.292745385747885	30.5	2.0	35.0	2.0	37.0
98-99	22.300630417859043	29.5	2.0	35.0	2.0	37.0
100-101	21.457036366268127	28.5	2.0	35.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.2544911041355178
1	2	0.12104414008016562
1	3	0.4213569484014812
1	4	0.3949868968340411
1	5	0.4302135154387372
1	6	0.398201426038824
1	7	0.34484961051122553
1	8	0.44316952514181907
1	9	0.41694921354176273
1	10-11	0.4506835165624068
1	12-13	0.4655552532965004
1	14-15	0.4765421443945428
1	16-17	0.019338529082354228
1	18-19	0.10316257445412447
1	20-21	0.18357974773618224
1	22-23	-0.13105548960272273
1	24-25	-0.09775642128981588
1	26-27	-0.7568732733435297
1	28-29	-0.6971804352725854
1	30-31	-1.2987299724096104
1	32-33	-1.056906667486878
1	34-35	-0.9412431137214696
1	36-37	-0.9376321479133622
1	38-39	-0.594896578697167
1	40-41	-0.5040821759262641
1	42-43	0.03198321924302405
1	44-45	0.024005561311689405
1	46-47	0.18388598827849023
1	48-49	0.15723933520013134
1	50-51	0.09854583981885412
1	52-53	-0.0874466301750445
1	54-55	-0.09040382489574483
1	56-57	-0.43299388711373865
1	58-59	-0.5471417079596108
1	60-61	-0.3515401749268747
1	62-63	-0.47394158110035534
1	64-65	-0.2814673582042886
1	66-67	0.04718110372543549
1	68-69	0.04633855186765601
1	70-71	0.04566010619086569
1	72-73	-0.030888053841060525
1	74-75	0.014370031221460522
1	76-77	-0.01739784198373684
1	78-79	-0.17575167933080493
1	80-81	-0.10691042840570475
1	82-83	-0.26233723818597454
1	84-85	-0.2639201271917493
1	86-87	-0.14071713355265913
1	88-89	0.39996311300652465
1	90-91	-0.6265708758186257
1	92-93	-0.8216859374068619
1	94-95	-0.859704872190779
1	96-97	-1.2715196360973522
1	98-99	-1.4367442506089425
1	100-101	-1.418975729036326
2	1	0.1621733874508564
2	2	0.20506960705009192
2	3	0.22981646876533546
2	4	0.17533507109398982
2	5	0.19964263843178287
2	6	0.20449584339765892
2	7	0.22371925240411628
2	8	0.17331640022256067
2	9	0.20434790577515116
2	10-11	0.22398802808002216
2	12-13	0.23163382719893377
2	14-15	0.17442639907050506
2	16-17	0.2329510954449816
2	18-19	0.25380330967875153
2	20-21	0.31057298217272944
2	22-23	0.3622320235649994
2	24-25	0.3716965684823279
2	26-27	0.4296217410512355
2	28-29	0.4298675271503285
2	30-31	0.4628081734288898
2	32-33	0.43363283490188564
2	34-35	0.3859167298156194
2	36-37	0.25949542723324015
2	38-39	0.25939127144823004
2	40-41	0.22566439022053686
2	42-43	0.0789984344218908
2	44-45	0.16270913058227165
2	46-47	0.17693917710604978
2	48-49	0.22169870724275853
2	50-51	0.27645486165395994
2	52-53	0.3948882894664152
2	54-55	0.39946425057286206
2	56-57	0.3475285734829825
2	58-59	0.297666837184984
2	60-61	0.27780282584519966
2	62-63	0.22965684226211636
2	64-65	0.167407155309796
2	66-67	-0.021653700334134385
2	68-69	-2.1933225033166082E-4
2	70-71	-0.029505688501714644
2	72-73	-0.02095623857064055
2	74-75	-0.011169881395744596
2	76-77	0.008877586661334647
2	78-79	0.04378095357189338
2	80-81	0.011273708738318788
2	82-83	0.02311732086319651
2	84-85	0.0654408067386214
2	86-87	0.5025196933837961
2	88-89	1.0219148158083975
2	90-91	0.38122357593322675
2	92-93	0.24081975925620824
2	94-95	0.24713757570520656
2	96-97	-0.2280765937543201
2	98-99	-0.34634961844453116
2	100-101	-0.2973905613439243
3	1	0.2602112813782611
3	2	0.32359009989847465
3	3	0.30743054021375116
3	4	0.29191212633032393
3	5	0.2887965522915934
3	6	0.28615338993170525
3	7	0.19653036368740118
3	8	0.283556329227558
3	9	0.2639264387095892
3	10-11	0.2567212774859797
3	12-13	0.24173234847147285
3	14-15	0.2838090494210732
3	16-17	0.3124867294881426
3	18-19	0.2602420726165846
3	20-21	0.34580637226211763
3	22-23	0.4003000037217248
3	24-25	0.47424429939251667
3	26-27	0.49016187189781135
3	28-29	0.5258370627717994
3	30-31	0.5803537891912143
3	32-33	0.5247920369849908
3	34-35	0.4948776302435647
3	36-37	0.42885485176257276
3	38-39	0.34569931601011916
3	40-41	0.41413235917326574
3	42-43	0.40221041205242614
3	44-45	0.3496112749930944
3	46-47	0.4157898450153894
3	48-49	0.40812858015875975
3	50-51	0.458577955599452
3	52-53	0.5102736830981769
3	54-55	0.5333191921169487
3	56-57	0.5401603143878688
3	58-59	0.5212734657594709
3	60-61	0.46880049978484095
3	62-63	0.44598065933791986
3	64-65	0.4535856734697319
3	66-67	0.43140197082910703
3	68-69	0.4304294606650174
3	70-71	0.4451962986969136
3	72-73	0.4776485880619745
3	74-75	0.4680232307811423
3	76-77	0.492398857599909
3	78-79	0.6076184661516102
3	80-81	0.7005830926731704
3	82-83	0.7444018233699872
3	84-85	0.80222410517624
3	86-87	1.2553575889722097
3	88-89	1.9096417039658355
3	90-91	1.2796931089774475
3	92-93	1.2162753938933335
3	94-95	1.2032061291386391
3	96-97	0.7870553582653734
3	98-99	0.7101319854924242
3	100-101	0.787482662879242
4	1	0.1817246784880453
4	2	0.19471018082421665
4	3	0.18797556155741546
4	4	0.1551555117643204
4	5	0.1308983095808287
4	6	0.16699360279865516
4	7	0.16627380837789474
4	8	0.14577739826331282
4	9	0.18799645504961404
4	10-11	0.13985071180945852
4	12-13	0.10893272316885572
4	14-15	0.10509980058667168
4	16-17	0.13298330151631177
4	18-19	0.17103561048487137
4	20-21	0.22970114595955238
4	22-23	0.2511552589773416
4	24-25	0.29212954504318134
4	26-27	0.3969788407112915
4	28-29	0.42407706684502244
4	30-31	0.43023902360436495
4	32-33	0.34537658777512803
4	34-35	0.37381094931836145
4	36-37	0.2790772374157555
4	38-39	0.2606003046739289
4	40-41	0.24132949419831107
4	42-43	0.20917986576875336
4	44-45	0.2225933279425405
4	46-47	0.3367929387218993
4	48-49	0.3134734045345269
4	50-51	0.2683592887415074
4	52-53	0.35920833591455903
4	54-55	0.39837415614786664
4	56-57	0.3447579475095708
4	58-59	0.40409495244713156
4	60-61	0.3833239566396358
4	62-63	0.36056165435724097
4	64-65	0.3470057973298424
4	66-67	0.38099497924347503
4	68-69	0.44249192555257366
4	70-71	0.5118846195749995
4	72-73	0.5274351230105694
4	74-75	0.5365311038190654
4	76-77	0.6349834648152495
4	78-79	0.6793795159347518
4	80-81	0.8226467081400735
4	82-83	0.8926941902249581
4	84-85	1.0914620599602607
4	86-87	1.6677933930266882
4	88-89	2.454048375932924
4	90-91	1.9005970226927857
4	92-93	1.9431754388293712
4	94-95	1.9661222477015095
4	96-97	1.6635093230822804
4	98-99	1.567857073700452
4	100-101	1.5944843636941357
5	1	0.1090153610608553
5	2	0.07913227415116353
5	3	0.09980946378617261
5	4	0.06990034483139596
5	5	0.07346951951357283
5	6	0.061277234122918856
5	7	0.05228629913770533
5	8	0.054787369149664755
5	9	0.001603462521366339
5	10-11	0.0728591136090273
5	12-13	0.018027960106230978
5	14-15	0.03343127385645772
5	16-17	0.022546096789490377
5	18-19	0.004675272531081021
5	20-21	-0.1881776900200265
5	22-23	-0.08954311791310943
5	24-25	0.1389841091560129
5	26-27	0.18043028746187417
5	28-29	0.24991227971199947
5	30-31	0.10320281141175514
5	32-33	0.1918947112522318
5	34-35	-0.1986449520556306
5	36-37	0.07155962079753664
5	38-39	0.036047950463192535
5	40-41	-0.05113977426390193
5	42-43	0.003116222282621095
5	44-45	0.01663781281850163
5	46-47	0.094710096700787
5	48-49	0.13922344628145567
5	50-51	0.05869779156236632
5	52-53	0.20044272925802176
5	54-55	0.24259554764600466
5	56-57	0.3179249766274026
5	58-59	0.3645310007715281
5	60-61	0.32632878337421545
5	62-63	0.28349563114872467
5	64-65	0.4053126021625104
5	66-67	0.4662259509652742
5	68-69	0.4983911614975085
5	70-71	0.5781363556405239
5	72-73	0.6450416536060573
5	74-75	0.7006981844008777
5	76-77	0.840113945999903
5	78-79	0.9444582192487125
5	80-81	1.1378385082747542
5	82-83	1.2722457894306558
5	84-85	1.4197970679979939
5	86-87	2.132849757410721
5	88-89	2.8055039376278934
5	90-91	2.4222762550217958
5	92-93	2.5465367015856906
5	94-95	2.595820198255101
5	96-97	2.3582536514998402
5	98-99	2.2599896114747473
5	100-101	2.195284787684738
6	1	-0.03344916593307801
6	2	-0.08404207428176136
6	3	-0.14462237430647207
6	4	-0.22262279428627352
6	5	-0.1608048003117588
6	6	-0.1498213101851178
6	7	-0.20167530685407087
6	8	-0.1653426566763656
6	9	-0.13161410350606673
6	10-11	-0.2159080670095932
6	12-13	-0.35611643834344875
6	14-15	-0.2584089857427898
6	16-17	-0.12419714579689156
6	18-19	-0.12439196934470687
6	20-21	-0.07019293200583832
6	22-23	0.004797866939846074
6	24-25	0.02403992861336235
6	26-27	0.12100201653915832
6	28-29	0.1266409676731044
6	30-31	0.22363807259849722
6	32-33	0.15969394367240852
6	34-35	0.1421718532159275
6	36-37	0.019554080361849913
6	38-39	0.03309470517856283
6	40-41	0.09438717939505636
6	42-43	0.03324933461654922
6	44-45	0.03936743316727842
6	46-47	-0.026795042030272498
6	48-49	0.022323105757656947
6	50-51	0.03287354675664744
6	52-53	0.07662395946837819
6	54-55	0.10620795619739454
6	56-57	0.22412483898342117
6	58-59	0.2373784323371666
6	60-61	0.24990751176246562
6	62-63	0.25165875557059536
6	64-65	0.29588615110269245
6	66-67	0.29262385561104765
6	68-69	0.2864074464408972
6	70-71	0.31926246069464526
6	72-73	0.32536630544942113
6	74-75	0.40548850545123116
6	76-77	0.5316085837042124
6	78-79	0.7184826924510013
6	80-81	0.9607124861969822
6	82-83	1.134941867798947
6	84-85	1.3371954035092521
6	86-87	2.0030151784747865
6	88-89	2.8186058955994078
6	90-91	2.4355375038476836
6	92-93	2.561573615988941
6	94-95	2.763495752438807
6	96-97	2.541804650511267
6	98-99	2.5515817999884334
6	100-101	2.6560347056723543
7	1	-0.03323035512810435
7	2	-0.13298614479605675
7	3	-0.185164445376806
7	4	-0.18249466142742676
7	5	-0.19746153286702395
7	6	-0.17140248453122808
7	7	-0.1165343279691271
7	8	-0.1363134274379334
7	9	-0.1565982506639827
7	10-11	-0.17250808651269267
7	12-13	-0.19208000889092602
7	14-15	-0.20251575596162041
7	16-17	-0.15805280280290646
7	18-19	-0.18475193192097805
7	20-21	-0.22142529569292435
7	22-23	-0.14321390584458982
7	24-25	-0.09018843008708188
7	26-27	0.09084831249731451
7	28-29	0.10235467479285631
7	30-31	0.1953603491629181
7	32-33	0.09097053704084601
7	34-35	0.009276880567696821
7	36-37	-0.07267891884971078
7	38-39	-0.0788601636367261
7	40-41	-0.09800375102268788
7	42-43	-0.17002607818887583
7	44-45	-0.12201527618876185
7	46-47	-0.046638677847582244
7	48-49	-0.03620549186843647
7	50-51	-0.07541894503388846
7	52-53	-0.0306413567937156
7	54-55	0.019623045331243816
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65	3	0.10182212714568095
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65	5	0.07530546505955016
65	6	0.04528248657496192
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65	8	0.08415552830062012
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65	18-19	-0.03474269807081498
65	20-21	0.11993833524826414
65	22-23	0.059524378029919944
65	24-25	0.012731293946714572
65	26-27	0.02991682928707462
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65	44-45	0.0067115835982747285
65	46-47	0.03639020594989262
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65	84-85	0.10313781776519448
65	86-87	0.5162236845482475
65	88-89	1.0468744095788303
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65	100-101	-0.318516590995106
66	1	-0.0999146320112132
66	2	-0.14906121599298672
66	3	-0.12827839895135185
66	4	-0.09778542134333179
66	5	-0.08942335941726043
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66	18-19	-0.1332712526681732
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66	78-79	0.1305136560012521
66	80-81	0.13399483690523084
66	82-83	0.27065345883594105
66	84-85	0.35594030243676045
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66	88-89	1.44836482327646
66	90-91	0.8318695823832236
66	92-93	0.8666622685604111
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66	96-97	0.5665140992850759
66	98-99	0.5402860017340352
66	100-101	0.5230940577868353
67	1	-0.2923566502749537
67	2	-0.19039680305331785
67	3	-0.20878237748753037
67	4	-0.20568742882146296
67	5	-0.218220932237692
67	6	-0.17397923713858177
67	7	-0.1588749261938176
67	8	-0.1706103589916168
67	9	-0.1665751114026648
67	10-11	-0.18928729179376802
67	12-13	-0.12738393445367535
67	14-15	-0.11566197269092271
67	16-17	-0.08740415365561915
67	18-19	-0.08035903112720888
67	20-21	-0.010585500791492564
67	22-23	0.050608291116262194
67	24-25	0.06503603188556895
67	26-27	0.21555440186351404
67	28-29	0.22943048150029455
67	30-31	0.28506522115649346
67	32-33	0.11848434952027276
67	34-35	0.19657452013031218
67	36-37	0.0746462030149857
67	38-39	0.08402543249056293
67	40-41	0.0858039692386896
67	42-43	0.027158726430585034
67	44-45	0.06618741192757227
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67	48-49	0.24143274007291637
67	50-51	0.12188701264665269
67	52-53	0.06712759015502456
67	54-55	0.12740130223316726
67	56-57	0.198382458727707
67	58-59	0.19210259017115305
67	60-61	0.24975101303500225
67	62-63	0.29224447765072625
67	64-65	0.28443632621406323
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67	70-71	0.3927430069725659
67	72-73	0.5833428593306849
67	74-75	0.6337604631746387
67	76-77	0.7732722702049557
67	78-79	0.9153720369758851
67	80-81	1.1232055935376266
67	82-83	1.324670098393998
67	84-85	1.4756462174773333
67	86-87	2.2505182831841175
67	88-89	2.9048458707533023
67	90-91	2.4733999119073076
67	92-93	2.6334660684131848
67	94-95	2.60718656411764
67	96-97	2.5788028364880056
67	98-99	2.5310162948195227
67	100-101	2.5618592365483153
68	1	-0.5214243831402996
68	2	-0.7371436703826646
68	3	-0.6407113176922081
68	4	-0.6625551584979661
68	5	-0.6063719439978073
68	6	-0.6463192302530345
68	7	-0.6571684057433629
68	8	-0.8108743003048104
68	9	-0.7369937571373342
68	10-11	-0.8100213840165651
68	12-13	-0.7311405578891055
68	14-15	-0.771369415612547
68	16-17	-0.6247179911656104
68	18-19	-0.6764868250465028
68	20-21	-0.5643106193942629
68	22-23	-0.6539577877102829
68	24-25	-0.5018654607443551
68	26-27	-0.6318269473225939
68	28-29	-0.46406587971048197
68	30-31	-0.7393658158966687
68	32-33	-0.4826427485198437
68	34-35	-0.4269005131006196
68	36-37	-0.36961013194275694
68	38-39	-0.4040229492666114
68	40-41	-0.3858373024899606
68	42-43	-0.5262606459504582
68	44-45	-0.40738475695495424
68	46-47	-0.5487103059519143
68	48-49	-0.41263268880585
68	50-51	-0.5344961343410972
68	52-53	-0.663308298478654
68	54-55	-0.6505247501816314
68	56-57	-0.44177144440696026
68	58-59	-0.34074991134389876
68	60-61	-0.2511106115701196
68	62-63	-0.15827841420395572
68	64-65	-0.04913664444895005
68	66-67	0.008538777417577137
68	68-69	0.1402748199705215
68	70-71	0.24366674488240392
68	72-73	0.4108990665810168
68	74-75	0.29063617471592806
68	76-77	0.49957647600801636
68	78-79	0.268143480960525
68	80-81	0.8310545296336542
68	82-83	1.1482861807446234
68	84-85	1.4677242634284262
68	86-87	2.4534123983346277
68	88-89	3.1093411158385216
68	90-91	2.7864143204101453
68	92-93	2.8984123798283363
68	94-95	3.1822223844985693
68	96-97	3.000630810505914
68	98-99	3.2385687342754004
68	100-101	3.0942251229315048
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1012360.0
3	115381.0
4	174846.0
5	189918.0
6	233831.0
7	244444.0
8	283988.0
9	262893.0
10	314975.0
11	314048.0
12	355344.0
13	312760.0
14	350835.0
15	369285.0
16	410948.0
17	477112.0
18	545000.0
19	644735.0
20	748856.0
21	886021.0
22	1017181.0
23	1178725.0
24	1348707.0
25	1563340.0
26	1826764.0
27	2140626.0
28	2527755.0
29	2998432.0
30	3577078.0
31	4312956.0
32	5099330.0
33	6264462.0
34	7989308.0
35	1.0656776E7
36	1.4611746E7
37	2.0122323E7
38	1.4729697E7
39	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.507105492550565	12.158674962549062	10.941250412581997	37.39296913231838
2	21.862403368107515	18.741535173164074	39.509818641076535	19.88624281765188
3	25.21277466872025	20.958291257580125	23.8503278723691	29.97860620133052
4	30.240842169813064	25.780302810614103	22.314197469993267	21.664657549579562
5	23.412967627143125	27.675500153350047	30.610538981916452	18.300993237590372
6	19.764838483044944	33.10513260724902	27.263996973623893	19.86603193608214
7	14.947423143238952	17.27571080807624	46.93377318535964	20.84309286332517
8	19.449215898559615	21.48468732896816	34.81596745982266	24.250129312649566
9	19.069973290465146	24.052882902819082	32.48344033972431	24.39370346699146
10-11	22.431594882908048	30.59060518775185	24.55267663881361	22.425123290526493
12-13	20.424642874813216	24.68067077820881	32.577831710199455	22.316854636778515
14-15	20.99973969918041	25.508944538540888	31.56695808166902	21.92435768060968
16-17	21.478323699979228	26.05233793606733	30.345797893261484	22.123540470691957
18-19	21.754482212616896	26.31852931938451	30.04844251205571	21.87854595594288
20-21	22.143457372346532	25.956582893662734	29.85853635199937	22.041423381991365
22-23	21.55805519164189	26.156689966491925	29.973875567644786	22.311379274221395
24-25	21.100285790838534	25.70962112282375	30.62823571455708	22.561857371780636
26-27	20.86883605209719	25.87069614751555	30.440352184114968	22.820115616272297
28-29	20.884925948387618	26.936223360917783	29.59693919258101	22.581911498113588
30-31	20.610347098148992	26.71970833893168	30.140222696988804	22.529721865930522
32-33	20.939238884557344	27.328803520600264	29.009072565731575	22.722885029110813
34-35	21.953111084457642	27.702633751370108	28.265078041565584	22.07917712260667
36-37	22.89244318750784	27.473587139577965	28.262671048384835	21.371298624529363
38-39	23.778977889250903	28.475891798882724	27.009474661362265	20.7356556505041
40-41	24.45717125787423	28.180100354993005	26.714802696956564	20.6479256901762
42-43	25.76872665853237	28.10685586037536	25.755960351334036	20.36845712975823
44-45	26.056007259770197	28.796065123256266	25.123268515274376	20.024659101699164
46-47	26.771164531325265	28.3031311115127	25.027501169172268	19.898203187989772
48-49	28.18491299176783	27.725504542831374	23.98029907927634	20.109283386124453
50-51	28.913640873461887	27.308659837259874	24.502872808227124	19.274826481051115
52-53	29.72804570896902	27.41242325343048	24.18843945516194	18.67109158243856
54-55	31.05048669059802	27.589271659341925	23.214660042911454	18.145581607148596
56-57	31.907434128036705	28.158078493861893	22.914139388821518	17.020347989279884
58-59	33.12139350321483	27.619971858311708	22.666893355711345	16.591741282762115
60-61	34.01774277373664	28.589609482782492	21.635939606738777	15.756708136742093
62-63	34.14796830333547	29.15913493863784	21.986247320374318	14.706649437652372
64-65	34.50511219958391	28.85642339010111	22.513786063814962	14.124678346500014
66-67	34.646887243836794	29.383470758259705	21.79356873783004	14.17607326007346
68-69	34.784552096089094	28.561312850387427	22.590709270667176	14.06342578285631
70-71	35.51081135206689	28.273135693835695	22.84912678408231	13.366926170015109
72-73	35.493786236616444	28.163868143392172	22.876625172202473	13.465720447788906
74-75	35.56641760394338	26.736207893104357	23.38997523136197	14.30739927159029
76-77	36.04238225626592	25.631116407067488	23.38598547733242	14.940515859334175
78-79	36.24086236494433	24.760117292451053	23.723199213388295	15.275821129216327
80-81	36.44661602047166	23.74696787177612	23.893120269018485	15.913295838733735
82-83	35.790040415615714	23.19726059248768	24.291633020486948	16.72106597140966
84-85	35.073377114476735	22.797846543873877	24.876402898271092	17.252373443378303
86-87	34.47416894686264	22.76626518327674	24.825902005542517	17.933663864318103
88-89	32.68793018773636	23.743196203654428	25.26971436995751	18.2991592386517
90-91	31.82825172750951	25.246264195271152	25.401624444449418	17.523859632769927
92-93	30.431782675191066	27.01877415705939	25.253768758201993	17.295674409547544
94-95	28.76977878480716	29.41831884693764	24.967228500639727	16.84467386761547
96-97	27.412965268954792	31.92553274560941	24.182012099186633	16.479489886249166
98-99	25.80604244777838	34.82760645268031	23.31877581159541	16.047575287945904
100-101	24.453051109570396	37.48342240686312	22.63950744529942	15.424019038267064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	998.0
1	789.0
2	510.5
3	446.5
4	466.0
5	654.0
6	845.0
7	978.5
8	1204.0
9	1449.5
10	1710.0
11	1940.5
12	2298.5
13	2748.5
14	3372.5
15	4185.0
16	5298.0
17	6919.0
18	9681.0
19	12304.5
20	14374.0
21	17305.5
22	21259.5
23	27623.0
24	36166.0
25	46425.5
26	59507.5
27	77614.5
28	104570.5
29	145417.5
30	209247.0
31	301947.5
32	456535.0
33	608065.0
34	703805.0
35	872806.5
36	1127720.0
37	1452180.0
38	1931998.5
39	2254720.5
40	2557741.5
41	3215190.0
42	4042833.0
43	4962968.5
44	5958160.5
45	6802128.0
46	7460560.0
47	7949080.0
48	7996711.5
49	7561748.5
50	6959560.0
51	6321437.0
52	5629275.0
53	4951832.0
54	4211444.0
55	3429390.0
56	2682696.0
57	2011133.0
58	1470965.0
59	1060147.0
60	750641.5
61	527536.0
62	365388.5
63	247002.0
64	166289.5
65	113185.0
66	78111.5
67	55179.0
68	39411.0
69	28205.5
70	20464.5
71	14904.0
72	10952.5
73	8231.5
74	6235.5
75	4678.5
76	3478.5
77	2653.5
78	2011.5
79	1467.0
80	1108.0
81	834.0
82	591.5
83	429.5
84	311.0
85	224.5
86	175.0
87	127.5
88	90.0
89	70.5
90	53.5
91	42.0
92	27.5
93	30.5
94	31.0
95	22.0
96	18.0
97	20.5
98	24.5
99	26.5
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09757929403038923
2	0.007597112175521401
3	0.009892668583507683
4	0.0018137617626737465
5	9.372765887153478E-4
6	0.00293250526111133
7	0.04080464932014193
8	0.0012357896552084254
9	8.873731885417329E-4
10-11	4.4368659427086645E-4
12-13	9.799213125000732E-5
14-15	5.6708409288198676E-5
16-17	0.0022941954061633657
18-19	2.1050161527779349E-4
20-21	0.0019131148957466707
22-23	0.0015719571054688673
24-25	0.003003277355903002
26-27	0.0032037982911460724
28-29	0.00502663339930593
30-31	0.005043419088455237
32-33	0.0012562046825521772
34-35	5.4440072916670735E-5
36-37	4.5366727430558945E-7
38-39	1.9054025520834755E-5
40-41	4.332522469618379E-4
42-43	8.624214884549255E-4
44-45	6.895742569444959E-5
46-47	3.1212308472224553E-4
48-49	1.0207513671875763E-4
50-51	3.629338194444715E-5
52-53	4.6546262343753476E-4
54-55	0.0012067549496528678
56-57	8.0435207734381E-4
58-59	2.56322009982658E-4
60-61	4.990340017361483E-6
62-63	8.619678211806199E-6
64-65	0.0
66-67	1.9099392248265315E-4
68-69	4.2100323055558697E-4
70-71	0.003466017975694703
72-73	0.07871580876476282
74-75	0.015905574637153964
76-77	0.008107034191840883
78-79	0.016127417934289397
80-81	0.00983414550512226
82-83	0.012762114093490536
84-85	0.020271214817796653
86-87	0.025864024975435957
88-89	0.13670265243195465
90-91	0.020489882444011946
92-93	0.01872148740876876
94-95	0.02091723701640781
96-97	0.02008430390078275
98-99	0.02174018945199815
100-101	0.021694822724567592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.10212931E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.05340643256077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98407546718764	16.781792671887743
2	14.886580222985751	7.459190894347663
3	5.437851339701949	4.08710099200194
4	2.8765078498412646	2.8826528107409874
5	1.7451540763240954	2.186102718079387
6	1.2201740921779876	1.8341710469889594
7	0.8832052986797839	1.5489110917851097
8	0.6873962399470093	1.377729390368519
9	0.5575348274186354	1.257133196846403
>10	3.9118216081929567	19.256367398533047
>50	0.45322435838924585	7.879280697465875
>100	0.31459801185372727	15.15262991745922
>500	0.026245766919283386	4.4975923067985155
>1k	0.013884118005146452	6.499376386357432
>5k	0.0010298137829192328	1.76525701016998
>10k+	7.16908592360955E-4	5.534711470169381
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT	580860	0.5270343459062894	No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA	324754	0.2946605239996748	No Hit
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT	171220	0.15535382141320603	Illumina Single End PCR Primer 1 (100% over 31bp)
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA	166563	0.1511283644203238	No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT	162938	0.14783927668160826	No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT	156941	0.14239799139358703	No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC	144393	0.13101275747761396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7220036458335364E-5	1.8146690972223578E-6	0.0	1.8146690972223578E-6	0.0
2	6.623542204861606E-5	5.444007291667073E-6	0.0	1.8146690972223578E-6	0.0
3	1.2158282951389796E-4	5.444007291667073E-6	0.0	1.8146690972223578E-6	1.8146690972223578E-6
4	1.8600358246529167E-4	5.444007291667073E-6	0.0	3.6293381944447156E-6	1.8146690972223578E-6
5	2.531463390625189E-4	5.444007291667073E-6	0.0	4.536672743055894E-6	1.8146690972223578E-6
6	3.084937465278008E-4	5.444007291667073E-6	0.0	5.444007291667073E-6	1.8146690972223578E-6
7	3.7472916857641685E-4	5.444007291667073E-6	0.0	6.351341840278252E-6	1.8146690972223578E-6
8	4.2553990329864287E-4	5.444007291667073E-6	0.0	6.351341840278252E-6	1.8146690972223578E-6
9	4.999413362847595E-4	5.444007291667073E-6	0.0	1.2702683680556504E-5	1.8146690972223578E-6
10-11	0.0010670254291667463	7.258676388889431E-6	0.0	1.678568914930681E-5	2.7220036458335367E-6
12-13	0.001491657997916778	1.1341681857639736E-5	9.073345486111789E-7	2.222969644097388E-5	3.1756709201391263E-6
14-15	0.006207075647049074	1.2702683680556504E-5	9.073345486111789E-7	2.7673703732640955E-5	3.6293381944447156E-6
16-17	0.011383419246875848	2.0415027343751524E-5	1.3610018229167683E-6	2.9942040104168903E-5	3.6293381944447156E-6
18-19	0.014115857240018416	2.99420401041689E-5	1.8146690972223578E-6	4.173738923611423E-5	4.083005468750305E-6
20-21	0.257505174234047	3.221037647569685E-5	1.8146690972223578E-6	5.1264401996531606E-5	5.897674565972663E-6
22-23	0.2841032328593094	3.266404375000244E-5	2.7220036458335367E-6	5.398640564236514E-5	6.351341840278252E-6
24-25	0.35087987996617204	3.538604739583598E-5	2.7220036458335367E-6	6.169874930556016E-5	6.351341840278252E-6
26-27	0.6328685696599431	3.7200716493058334E-5	2.7220036458335367E-6	6.668908932292164E-5	6.351341840278252E-6
28-29	1.4799769729379577	4.083005468750305E-5	2.7220036458335367E-6	7.167942934028312E-5	6.351341840278252E-6
30-31	2.798052344692657	4.355205833333659E-5	2.7220036458335367E-6	7.394776571181108E-5	6.351341840278252E-6
32-33	4.804614079268067	4.5366727430558944E-5	2.7220036458335367E-6	8.075277482639493E-5	6.351341840278252E-6
34-35	7.374911842241088	4.672772925347571E-5	2.7220036458335367E-6	8.619678211806199E-5	6.351341840278252E-6
36-37	10.71716348783066	4.8088731076392476E-5	2.7220036458335367E-6	8.801145121528434E-5	7.258676388889431E-6
38-39	14.572082290416539	4.899606562500366E-5	2.7220036458335367E-6	9.02797875868123E-5	7.258676388889431E-6
40-41	18.63659446639705	5.0357067447920424E-5	2.7220036458335367E-6	9.254812395834025E-5	7.258676388889431E-6
42-43	22.78991382599198	6.532808750000488E-5	2.7220036458335367E-6	9.572379487847937E-5	7.258676388889431E-6
44-45	26.981177009075278	9.753846397570173E-5	2.7220036458335367E-6	9.708479670139613E-5	7.71234366319502E-6
46-47	31.214945186422817	1.5243220416667806E-4	2.7220036458335367E-6	9.708479670139613E-5	8.16601093750061E-6
48-49	35.34684237732503	1.5515420781251157E-4	2.7220036458335367E-6	9.753846397570173E-5	8.16601093750061E-6
50-51	39.381931508563184	1.5787621145834513E-4	2.7220036458335367E-6	9.84457985243129E-5	8.16601093750061E-6
52-53	43.19800142144845	1.587835460069563E-4	2.7220036458335367E-6	1.0207513671875763E-4	8.16601093750061E-6
54-55	46.803782489007574	1.5969088055556746E-4	2.7220036458335367E-6	1.0343613854167439E-4	8.16601093750061E-6
56-57	50.24563723833821	1.619592169270954E-4	2.7220036458335367E-6	1.0343613854167439E-4	8.16601093750061E-6
58-59	53.468399275217536	1.637738860243178E-4	2.7220036458335367E-6	1.0661180946181352E-4	8.16601093750061E-6
60-61	56.523709999147016	1.6513488784723456E-4	2.7220036458335367E-6	1.0888014583334146E-4	8.16601093750061E-6
62-63	59.42108734954159	1.6694955694445692E-4	2.7220036458335367E-6	1.0888014583334146E-4	8.16601093750061E-6
64-65	62.08582412167226	1.6921789331598487E-4	2.7220036458335367E-6	1.0888014583334146E-4	8.16601093750061E-6
66-67	64.57906150776446	1.7375456605904076E-4	2.7220036458335367E-6	1.0888014583334146E-4	8.16601093750061E-6
68-69	66.9634632074162	1.7738390425348546E-4	2.7220036458335367E-6	1.0933381310764705E-4	8.16601093750061E-6
70-71	69.12727100960595	1.8373524609376372E-4	2.7220036458335367E-6	1.1024114765625822E-4	8.16601093750061E-6
72-73	71.12422407131156	1.9144758975695873E-4	2.7220036458335367E-6	1.11602149479175E-4	8.16601093750061E-6
74-75	73.0054379916636	1.9870626614584816E-4	2.7220036458335367E-6	1.1432415312500853E-4	8.16601093750061E-6
76-77	74.6767727282382	2.0233560434029286E-4	2.7220036458335367E-6	1.1523148767361972E-4	8.16601093750061E-6
78-79	76.18639322821384	2.0868694618057115E-4	2.7220036458335367E-6	1.2294383133681474E-4	9.073345486111788E-6
80-81	77.56687688489112	2.1322361892362705E-4	2.7220036458335367E-6	1.2657316953125944E-4	9.073345486111788E-6
82-83	78.80600734590753	2.2002862803821086E-4	2.7220036458335367E-6	1.283878386284818E-4	9.073345486111788E-6
84-85	79.96940712882412	2.2864830625001707E-4	2.7220036458335367E-6	1.3156350954862092E-4	9.073345486111788E-6
86-87	81.01576801364624	2.4180465720487917E-4	2.7220036458335367E-6	1.3473918046876007E-4	9.073345486111788E-6
88-89	81.99533365100325	2.48609666319463E-4	2.7220036458335367E-6	1.3519284774306565E-4	9.073345486111788E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATT	66740	0.0	82.02306	1
GGCATTC	82280	0.0	64.314445	2
GCATTCC	108090	0.0	51.298904	3
ATTCCTG	116820	0.0	46.375835	5
CTGCTGA	115925	0.0	44.948772	9
TCCTGCT	119640	0.0	44.85316	7
TTCCTGC	121320	0.0	44.600803	6
CCTGCTG	124710	0.0	41.980755	8
TGAACCG	54510	0.0	39.70513	12-13
CGGGATT	10855	0.0	39.68896	1
GAACCGA	35960	0.0	38.531586	14-15
CCAACGA	15180	0.0	38.505238	8
AACCGAG	37870	0.0	36.619564	14-15
CACGCGT	37335	0.0	36.375874	1
CAACGAA	16450	0.0	35.820847	9
ACGCGTC	41645	0.0	34.23416	2
ACCGCTC	16510	0.0	33.881035	16-17
GAACCGC	23045	0.0	33.586296	14-15
CATTCCT	161615	0.0	33.189796	4
TCGTCGC	10945	0.0	31.836138	86-87
>>END_MODULE