FastQCFastQC Report
Fri 27 May 2016
SRR1233080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110212931
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT5043570.4576205309338883No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG3825390.34709085089117175Illumina Paired End PCR Primer 2 (96% over 33bp)
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA3260710.2958554836001957No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG3020280.2740404390479371Illumina Paired End PCR Primer 2 (100% over 31bp)
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1611700.14623510919966368No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA1604320.14556549630278864No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1158700.10513285414757728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAT159150.036.502231
CGCGCGT30750.036.2952271
GCGCGTC43200.032.0981452
GTTTATT872650.029.1590331
GCGGATT185300.028.6997591
GCGAATT67450.027.5984652
CGGGCAT126200.024.5659071
CGGGGCT177350.024.5268861
CGGCGGT37000.024.3634531
CGGATTT202850.023.9486712
GGCGAAT95150.023.810231
CTTCGAC60050.023.408048
CCCGCGT68800.023.3625491
CGGGCGT48600.022.7682931
CCCGGGT72050.022.6397111
CGAATTT79950.021.4422113
CGGGGAT103450.021.1622071
GTCGGGT111900.021.1412351
GGAGAAT1242700.020.9865461
CGGGTGT129150.020.75491
Produced by FastQC (version 0.11.5)