##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233080_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110212931 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.20996353867043 39.0 37.0 39.0 34.0 39.0 2 36.94328684535211 39.0 37.0 39.0 33.0 39.0 3 36.91381211883386 39.0 37.0 39.0 33.0 39.0 4 36.88274077385711 39.0 37.0 39.0 33.0 39.0 5 36.842549528058555 39.0 37.0 39.0 33.0 39.0 6 36.922331972098625 39.0 37.0 39.0 33.0 39.0 7 36.871211173941106 39.0 37.0 39.0 33.0 39.0 8 36.83747306384584 39.0 37.0 39.0 33.0 39.0 9 36.82172223511595 39.0 37.0 39.0 33.0 39.0 10-11 36.792857822645146 39.0 36.0 39.0 33.0 39.0 12-13 36.48592480949445 38.0 36.0 39.0 31.5 39.0 14-15 36.58374443830007 38.0 36.0 39.0 32.0 39.0 16-17 36.50088146190396 38.0 36.0 39.0 32.0 39.0 18-19 36.4162171678385 38.0 36.0 39.0 31.0 39.0 20-21 36.42228734938553 38.0 36.0 39.0 31.5 39.0 22-23 36.33523032338193 38.0 36.0 39.0 31.0 39.0 24-25 36.21505562718407 38.0 36.0 39.0 31.0 39.0 26-27 35.996713148840946 38.0 36.0 39.0 31.0 39.0 28-29 35.82283505825646 38.0 35.5 39.0 30.5 39.0 30-31 35.62254793858989 38.0 35.0 39.0 30.0 39.0 32-33 35.45384863687184 38.0 35.0 39.0 29.5 39.0 34-35 35.28025237800817 37.5 35.0 39.0 29.0 39.0 36-37 35.041767916507 37.0 35.0 39.0 28.5 39.0 38-39 35.05951216831353 37.0 35.0 39.0 29.0 39.0 40-41 35.11324046903353 37.0 35.0 39.0 28.5 39.0 42-43 35.088254703071094 37.0 35.0 39.0 29.0 39.0 44-45 34.93557530467999 37.0 35.0 39.0 28.5 39.0 46-47 34.80841489915552 37.0 35.0 39.0 28.0 39.0 48-49 34.6463491656891 37.0 34.0 39.0 28.0 39.0 50-51 34.42545073499588 37.0 34.0 39.0 27.0 39.0 52-53 34.183764153772486 37.0 34.0 39.0 27.0 39.0 54-55 33.932733868587526 37.0 33.5 39.0 26.0 39.0 56-57 33.5848957732555 36.5 33.0 39.0 25.0 39.0 58-59 33.21762750325549 36.0 33.0 39.0 24.5 39.0 60-61 32.94173059874436 36.0 33.0 39.0 23.5 39.0 62-63 32.54250387370607 36.0 32.0 37.5 22.0 39.0 64-65 32.155468372399966 36.0 31.5 37.0 20.5 39.0 66-67 31.53037787371792 35.5 31.0 37.0 17.0 39.0 68-69 30.95511385592313 35.0 30.0 37.0 13.5 39.0 70-71 30.127247564081205 34.5 29.5 37.0 2.0 39.0 72-73 29.24812025006394 34.0 27.5 36.5 2.0 39.0 74-75 28.589843831482895 34.0 26.5 36.0 2.0 38.0 76-77 29.022167276360705 35.0 27.5 37.0 2.0 39.0 78-79 28.273422004356277 34.0 25.5 37.0 2.0 39.0 80-81 27.51954834138292 34.0 23.5 37.0 2.0 39.0 82-83 26.626886903134807 33.0 20.5 37.0 2.0 39.0 84-85 25.64032200087302 33.0 11.5 36.5 2.0 39.0 86-87 23.869682650940476 31.5 2.0 36.0 2.0 38.0 88-89 22.80621503478571 30.5 2.0 35.5 2.0 37.0 90-91 21.15447811654696 29.0 2.0 35.0 2.0 37.0 92-93 19.60522485787081 26.0 2.0 34.0 2.0 37.0 94-95 18.44475356979663 22.5 2.0 34.0 2.0 37.0 96-97 16.671180820878448 7.5 2.0 33.0 2.0 36.0 98-99 15.729273432533972 2.0 2.0 32.0 2.0 36.0 100-101 15.016856175433716 2.0 2.0 31.5 2.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 0.3141969768732409 1 2 0.39620007793907774 1 3 0.4274390520307918 1 4 0.4214805513288695 1 5 0.4477908513078859 1 6 0.44571281943357377 1 7 0.44539363710212854 1 8 0.4612213956233191 1 9 0.44796075954909753 1 10-11 0.49923462059527424 1 12-13 0.7634509579476969 1 14-15 0.5413105972560643 1 16-17 -0.010801822554071805 1 18-19 0.09102743105909639 1 20-21 0.43498886755450883 1 22-23 0.42478092906993936 1 24-25 0.4787975789331185 1 26-27 0.6198834796284487 1 28-29 0.5629909524282724 1 30-31 0.6465388951897353 1 32-33 0.4768326761670636 1 34-35 0.44218503185724245 1 36-37 0.5402227323154918 1 38-39 0.2974028607207586 1 40-41 0.09404885835124333 1 42-43 -0.055811592244737085 1 44-45 -0.05399648994090711 1 46-47 -0.2047474310829287 1 48-49 0.11363790613972213 1 50-51 0.3673170518638287 1 52-53 0.658388637092834 1 54-55 0.6397045270824293 1 56-57 0.704482600030552 1 58-59 0.7001534994692094 1 60-61 0.7139405170372513 1 62-63 0.5510402683835025 1 64-65 0.5569760559877324 1 66-67 0.41176727106442 1 68-69 0.4104521179108822 1 70-71 0.21952712975613053 1 72-73 0.2354967761836413 1 74-75 0.33370753314311585 1 76-77 0.38093090987326406 1 78-79 0.30579725383566725 1 80-81 0.21023461129970755 1 82-83 -0.11154693293300255 1 84-85 -0.11746809330611185 1 86-87 -0.4487809452675222 1 88-89 -0.604553103504351 1 90-91 -0.9966253847221758 1 92-93 -1.0886028706000133 1 94-95 -1.203662219132422 1 96-97 -1.3830231084445401 1 98-99 -1.3309717784971813 1 100-101 -1.243450048583556 2 1 0.23791476003654566 2 2 0.298184110048787 2 3 0.3175231420819742 2 4 0.3153005851108617 2 5 0.3552781225746102 2 6 0.3285467871714687 2 7 0.33963242113456715 2 8 0.354588655084882 2 9 0.33444236953138784 2 10-11 0.36033295228301654 2 12-13 0.6145188239754944 2 14-15 0.4879401441547131 2 16-17 0.4214852391854649 2 18-19 0.4407403131142047 2 20-21 0.43390470284143845 2 22-23 0.43300687081305966 2 24-25 0.4187595891253153 2 26-27 0.4346761724234156 2 28-29 0.4919511695847092 2 30-31 0.5809648344762053 2 32-33 0.5718686290793684 2 34-35 0.5878890460625215 2 36-37 0.6481675618511034 2 38-39 0.6937600111680169 2 40-41 0.6260092686644612 2 42-43 0.5926114591208318 2 44-45 0.6207464573149224 2 46-47 0.5925537357791271 2 48-49 0.46527222550376024 2 50-51 0.47376281754080907 2 52-53 0.4354784841899928 2 54-55 0.39399361531111765 2 56-57 0.3058380042665121 2 58-59 0.43112472275593916 2 60-61 0.4795659223955653 2 62-63 0.5429404463604257 2 64-65 0.4022787888406256 2 66-67 0.5364892031050168 2 68-69 0.5518074207532067 2 70-71 0.505363813968426 2 72-73 0.507899110029701 2 74-75 0.3895427856706526 2 76-77 0.27980926281497887 2 78-79 0.24224366385405105 2 80-81 0.17023896656211335 2 82-83 0.12438783834441125 2 84-85 0.060200985474963886 2 86-87 -0.030166038346106205 2 88-89 -0.005889464012533097 2 90-91 -0.20434615928797584 2 92-93 -0.24827732590348361 2 94-95 -0.34645667761782306 2 96-97 -0.5105430792968555 2 98-99 -0.5200022898914991 2 100-101 -0.5114207242882838 3 1 0.15363521703120142 3 2 0.17886113318282781 3 3 0.18772036790169722 3 4 0.21139228625496997 3 5 0.1978486756093787 3 6 0.19810766787070833 3 7 0.2504938381960429 3 8 0.258039897491777 3 9 0.23348517801795765 3 10-11 0.247467295149562 3 12-13 0.4943545265801177 3 14-15 0.3317142161345217 3 16-17 0.387236654907575 3 18-19 0.422102447123784 3 20-21 0.32935802978364137 3 22-23 0.18869458503599645 3 24-25 0.23179267572366058 3 26-27 0.2671995777603442 3 28-29 0.2662071432511368 3 30-31 0.32118802343234165 3 32-33 0.36527529227636535 3 34-35 0.39154947391521233 3 36-37 0.393278461026469 3 38-39 0.4314767642656747 3 40-41 0.32829669949344975 3 42-43 0.3963319696944083 3 44-45 0.39751874405786225 3 46-47 0.33768746172606967 3 48-49 0.23646472120837814 3 50-51 -0.11627122477501217 3 52-53 -0.13240758229371608 3 54-55 0.03219977039634614 3 56-57 0.21571846599125166 3 58-59 0.24725587983394348 3 60-61 0.3017335909249965 3 62-63 0.3581441287200491 3 64-65 0.23595804457493585 3 66-67 0.20947339637228168 3 68-69 0.38708106724894265 3 70-71 0.30521181718566126 3 72-73 0.43915364807228485 3 74-75 0.3715838506602225 3 76-77 0.4566642823961722 3 78-79 0.5998577151029387 3 80-81 0.7601575193911607 3 82-83 0.8717018257667419 3 84-85 0.8841966958138201 3 86-87 0.8823741531595743 3 88-89 0.904127601349515 3 90-91 0.7640008746042817 3 92-93 0.6475303745095324 3 94-95 0.5257183925411084 3 96-97 0.4178606988876403 3 98-99 0.35075291004581644 3 100-101 0.3735506805784663 4 1 0.06684055217951368 4 2 0.07017614826699514 4 3 0.08332294947818752 4 4 0.10213264069101058 4 5 0.11101579323064925 4 6 0.11148600536400721 4 7 0.13315088250869422 4 8 0.1444640426320376 4 9 0.13634768997909674 4 10-11 0.16666299145002483 4 12-13 0.4134138306825932 4 14-15 0.2533699560817908 4 16-17 0.22754559303457 4 18-19 0.22800132646555227 4 20-21 0.19514759742749987 4 22-23 0.1560251093623748 4 24-25 0.14189779816658188 4 26-27 0.19704418196839413 4 28-29 0.2036737653931695 4 30-31 0.24310546765744334 4 32-33 0.2299911429179673 4 34-35 0.2436360936291706 4 36-37 0.20284620049881852 4 38-39 0.2264411875270511 4 40-41 0.19356833412236085 4 42-43 0.22890705628685026 4 44-45 0.22911380384790903 4 46-47 0.11528504246492588 4 48-49 0.14578587942026644 4 50-51 0.1738263440298411 4 52-53 0.19229159731288803 4 54-55 0.1696395500895207 4 56-57 0.22371699156990843 4 58-59 0.2154114645799865 4 60-61 0.23485085551409668 4 62-63 0.2547490749334216 4 64-65 0.2799463855423525 4 66-67 0.22697386043502732 4 68-69 0.3184682506546572 4 70-71 0.3154587410321348 4 72-73 0.3673584804224035 4 74-75 0.323975782588235 4 76-77 0.40262366446908615 4 78-79 0.5736926095491448 4 80-81 0.7625948813024763 4 82-83 0.916917214280673 4 84-85 1.0126983263988407 4 86-87 0.9740199598067676 4 88-89 1.046140190052931 4 90-91 1.0766990824561802 4 92-93 1.1018326774732863 4 94-95 1.0858303274818404 4 96-97 1.0129307758368888 4 98-99 0.9367096824761472 4 100-101 0.9645714440943767 5 1 -0.018943087760099786 5 2 -0.034998972089475444 5 3 -0.00360292233812487 5 4 -0.04324531411513988 5 5 -0.03911675632296863 5 6 0.01402644181090551 5 7 -0.012761969439104348 5 8 0.01600584116912529 5 9 -0.014656701617155932 5 10-11 0.05624444344559265 5 12-13 0.2477535620106508 5 14-15 0.07288993029265356 5 16-17 -0.26481513398492496 5 18-19 -0.19991608978178022 5 20-21 -0.07956650349541405 5 22-23 -0.05479805017655082 5 24-25 -0.09449085954869219 5 26-27 -0.06608080138476424 5 28-29 -0.005780842645343398 5 30-31 0.019797920271479086 5 32-33 -0.19887045132227854 5 34-35 -0.04459560052142564 5 36-37 -0.11285680269769216 5 38-39 -0.0933587104047362 5 40-41 -0.37226466599796737 5 42-43 -0.12240306230446407 5 44-45 -0.1402062145936256 5 46-47 -0.061833302360078335 5 48-49 -0.039453639535445006 5 50-51 -0.05254277385042627 5 52-53 -0.07442391107640844 5 54-55 -0.1685138247216642 5 56-57 -0.0345365900661605 5 58-59 -0.010376067793771426 5 60-61 0.07319041971032192 5 62-63 0.06763196966247875 5 64-65 0.10818842386721172 5 66-67 0.028882740735465262 5 68-69 0.1666940955874665 5 70-71 0.21552084525800907 5 72-73 0.28237851829080896 5 74-75 0.31208165682597055 5 76-77 0.3878882344916228 5 78-79 0.5613507038983094 5 80-81 0.6447442523823632 5 82-83 0.8212200433113885 5 84-85 0.8480604134432461 5 86-87 1.455086736747539 5 88-89 1.6088904649366995 5 90-91 1.7888461503372177 5 92-93 1.9281891153321666 5 94-95 1.8744085909085264 5 96-97 1.8286327743692645 5 98-99 1.7064768107059596 5 100-101 1.6557565778556143 6 1 -0.1733956010038895 6 2 -0.23637650232783614 6 3 -0.22347190728751798 6 4 -0.22947930682524742 6 5 -0.20507098775896537 6 6 -0.20333399178370115 6 7 -0.19741243023680255 6 8 -0.22482476793115325 6 9 -0.22988146004908572 6 10-11 -0.23026547812596476 6 12-13 -0.08774988713273757 6 14-15 -0.19817516180989259 6 16-17 -0.12318141641316771 6 18-19 -0.11620658119898053 6 20-21 -0.17758819457073827 6 22-23 -0.2103286729504532 6 24-25 -0.21471946963433908 6 26-27 -0.25823609737284414 6 28-29 -0.2675057730094892 6 30-31 -0.23964910408271578 6 32-33 -0.25494790491062247 6 34-35 -0.2717996624432999 6 36-37 -0.21306650589912834 6 38-39 -0.1793842359261646 6 40-41 -0.12901493491337845 6 42-43 -0.14894145246640278 6 44-45 -0.1704353415866322 6 46-47 -0.1313163817499614 6 48-49 -0.1911535831739144 6 50-51 -0.1392350036497092 6 52-53 -0.13199861104256172 6 54-55 -0.19390672752031435 6 56-57 -0.2397769673401271 6 58-59 -0.26736813064319165 6 60-61 -0.2326130832201656 6 62-63 -0.15749437268400612 6 64-65 -0.15853395859679154 6 66-67 -0.18505771711270214 6 68-69 -0.011851824067779404 6 70-71 0.1703771979732167 6 72-73 0.25719787636197466 6 74-75 0.28294008582238916 6 76-77 0.4592485764657823 6 78-79 0.7141843738619684 6 80-81 1.0249141269213986 6 82-83 1.3412187375397124 6 84-85 1.4347012274904465 6 86-87 1.8449120812019544 6 88-89 2.0830173071402385 6 90-91 2.30967926330381 6 92-93 2.420032395121904 6 94-95 2.478285081414054 6 96-97 2.50223220294313 6 98-99 2.376936951969567 6 100-101 2.237843616788046 7 1 -0.2703872860902905 7 2 -0.350412399457376 7 3 -0.3630684921747971 7 4 -0.35350428210252005 7 5 -0.3441912710752959 7 6 -0.3547569893344047 7 7 -0.45148632621296514 7 8 -0.4005331855881522 7 9 -0.4134777849602642 7 10-11 -0.36333843506297114 7 12-13 -0.16023630596036043 7 14-15 -0.34211625211431596 7 16-17 -0.3463317952863534 7 18-19 -0.36608672960831967 7 20-21 -0.4016354308016048 7 22-23 -0.44874769044309915 7 24-25 -0.48138743816979 7 26-27 -0.5112762933440607 7 28-29 -0.5873930105107306 7 30-31 -0.5281411648102079 7 32-33 -0.5451123497628814 7 34-35 -0.5896278603725946 7 36-37 -0.5164392769501802 7 38-39 -0.48082119021577796 7 40-41 -0.42249489121610395 7 42-43 -0.3637186591611794 7 44-45 -0.417223416315295 7 46-47 -0.36572810630163843 7 48-49 -0.41342608972326644 7 50-51 -0.34887092837301026 7 52-53 -0.3896493565959176 7 54-55 -0.4783702261201057 7 56-57 -0.42108618183278423 7 58-59 -0.37393634699739664 7 60-61 -0.5509169225354498 7 62-63 -0.5495912969677548 7 64-65 -0.5520420777264512 7 66-67 -0.32241946088684514 7 68-69 -0.2922988842130394 7 70-71 -0.141836618509803 7 72-73 0.0019831319046019757 7 74-75 0.10296888608687738 7 76-77 0.686648950724333 7 78-79 0.8256626861156171 7 80-81 1.2345212436063377 7 82-83 1.522096060737777 7 84-85 1.745770676671647 7 86-87 2.3922167535343632 7 88-89 2.5371913220947455 7 90-91 2.8755924484456123 7 92-93 3.027490038712827 7 94-95 2.9855744192613187 7 96-97 3.155506497692617 7 98-99 2.9948497625250514 7 100-101 2.8706383192746276 8 1 -0.9248054124081833 8 2 -0.8919004410197431 8 3 -1.2171023415922093 8 4 -0.9663499488278973 8 5 -1.045749963139258 8 6 -1.0433563454299986 8 7 -1.0366913515405258 8 8 -1.1633831563068 8 9 -1.142811333552693 8 10-11 -0.9889678403865574 8 12-13 -0.846530129406581 8 14-15 -1.0940802309291797 8 16-17 -1.112206587700335 8 18-19 -1.2623509496702994 8 20-21 -0.953378204178108 8 22-23 -1.0133675704086755 8 24-25 -0.918216324071004 8 26-27 -1.0316436320503541 8 28-29 -0.9951308589571468 8 30-31 -1.1292463409335198 8 32-33 -1.121285283504477 8 34-35 -1.2917206162046355 8 36-37 -1.5246202087320953 8 38-39 -1.3825651755833874 8 40-41 -1.4556297789801107 8 42-43 -1.8685940491114366 8 44-45 -1.3902891538753863 8 46-47 -1.6423323930029952 8 48-49 -1.299303885561578 8 50-51 -1.2951837866308864 8 52-53 -1.0125419845358365 8 54-55 -0.8858227330168802 8 56-57 -1.0211943994155632 8 58-59 -1.1271986041742394 8 60-61 -1.0349676534748724 8 62-63 -1.1068661057280238 8 64-65 -1.1583993173589455 8 66-67 -1.151677520733518 8 68-69 -0.9679534483123824 8 70-71 -0.7429720664054678 8 72-73 -0.7799311219614999 8 74-75 -0.3616548171523739 8 76-77 0.1459549625737786 8 78-79 0.2808364200949178 8 80-81 0.6294767871591951 8 82-83 1.212032975929727 8 84-85 1.339452145656395 8 86-87 2.169368375587865 8 88-89 2.3404908645965428 8 90-91 2.9114224789039334 8 92-93 3.049847103506597 8 94-95 2.9802284609688847 8 96-97 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0.10113378793433014 65 54-55 0.10591939046505416 65 56-57 -0.009623720137568625 65 58-59 0.08918940173541046 65 60-61 0.016407512640356003 65 62-63 0.033587208949839464 65 64-65 -0.040574290907585464 65 66-67 -0.02111158483208797 65 68-69 -0.056895180504952236 65 70-71 -0.0367302946614565 65 72-73 -0.1500425182809586 65 74-75 -0.1988603052763196 65 76-77 -0.460197711350407 65 78-79 -0.5650843322384453 65 80-81 -0.627770767790981 65 82-83 -0.8331877855500949 65 84-85 -0.889719328181588 65 86-87 -1.1445369984461387 65 88-89 -1.2162110357582456 65 90-91 -1.214530701057388 65 92-93 -1.3511460503987607 65 94-95 -1.337524511973772 65 96-97 -1.3991688103664934 65 98-99 -1.3691515374559007 65 100-101 -1.298058117878357 66 1 -0.11055786563050418 66 2 -0.15267180775919087 66 3 -0.13363401994923407 66 4 -0.1402742775057888 66 5 -0.13274143801070437 66 6 -0.1335240916927205 66 7 -0.1468238789390668 66 8 -0.14244695753291126 66 9 -0.15801494084494294 66 10-11 -0.11339527007008599 66 12-13 0.09098831556205056 66 14-15 -0.09139658893241176 66 16-17 -0.08164366476440676 66 18-19 -0.08635912071925844 66 20-21 -0.20060294103446097 66 22-23 -0.2318715521958623 66 24-25 -0.21564308857411874 66 26-27 -0.2552770460538625 66 28-29 -0.2816813448791464 66 30-31 -0.26516567611684394 66 32-33 -0.26672998668435355 66 34-35 -0.2745596286398424 66 36-37 -0.2456766684379872 66 38-39 -0.2212260125602441 66 40-41 -0.1972828928345436 66 42-43 -0.15819198359313447 66 44-45 -0.1800937706460175 66 46-47 -0.29588718079927645 66 48-49 -0.3607709410702 66 50-51 -0.3002454329954034 66 52-53 -0.2762067249233269 66 54-55 -0.310132108382291 66 56-57 -0.409516585275135 66 58-59 -0.3939304409833042 66 60-61 -0.35520375667070425 66 62-63 -0.38656198030340505 66 64-65 -0.42581114428826794 66 66-67 -0.358947784950562 66 68-69 -0.35700842378589215 66 70-71 -0.28086624939929905 66 72-73 -0.17173983434021878 66 74-75 -0.27788372043466936 66 76-77 -0.27938826437301145 66 78-79 -0.26849912697688794 66 80-81 -0.27349458595588416 66 82-83 -0.29132006676547917 66 84-85 -0.3204328834407626 66 86-87 -0.3653570356145579 66 88-89 -0.35691116938997425 66 90-91 -0.4228519071005046 66 92-93 -0.5286268701009185 66 94-95 -0.5830822108547586 66 96-97 -0.6569195652376578 66 98-99 -0.6847414144943205 66 100-101 -0.7014079339080581 67 1 -0.23180427688713223 67 2 -0.2543444110426165 67 3 -0.2861370714084899 67 4 -0.2576265104836821 67 5 -0.25869811163834555 67 6 -0.3116297306390763 67 7 -0.2771158315604296 67 8 -0.29421082297369594 67 9 -0.2903622391898608 67 10-11 -0.25583520322180675 67 12-13 -0.06172875368948638 67 14-15 -0.2738926124466303 67 16-17 -0.22484845553973543 67 18-19 -0.2275734481091618 67 20-21 -0.2694799436910671 67 22-23 -0.30166757975017333 67 24-25 -0.308660112870875 67 26-27 -0.3379525353303805 67 28-29 -0.35393198174335083 67 30-31 -0.39404877630470025 67 32-33 -0.37106839824219406 67 34-35 -0.4046671533898447 67 36-37 -0.34962714152707974 67 38-39 -0.3192610854055502 67 40-41 -0.2863164142242596 67 42-43 -0.18879992277841495 67 44-45 -0.20160273284532337 67 46-47 -0.2044911127166884 67 48-49 -0.2306879738643488 67 50-51 -0.16846285854472143 67 52-53 -0.1762103200159828 67 54-55 -0.17952373119776155 67 56-57 -0.1765611491183776 67 58-59 -0.1490175820169526 67 60-61 -0.18760456156527283 67 62-63 -0.2642784086530199 67 64-65 -0.1372688687906276 67 66-67 -0.09310923098940549 67 68-69 -0.027584102800243926 67 70-71 0.024985992325628814 67 72-73 0.17089698833380496 67 74-75 0.03639607314080706 67 76-77 0.28135771933558473 67 78-79 0.3596730012169367 67 80-81 0.588529420765191 67 82-83 0.7188350354434618 67 84-85 0.7419595159626056 67 86-87 0.868490438278755 67 88-89 0.9016344455966703 67 90-91 0.9339268591616694 67 92-93 0.9796233703178139 67 94-95 1.022449886839265 67 96-97 0.9156244815763905 67 98-99 0.8487120152236702 67 100-101 0.7114090608067727 68 1 -0.5585557623533575 68 2 -0.7249599397814563 68 3 -0.730661868481036 68 4 -0.7267484689381334 68 5 -0.8709717424934169 68 6 -0.7629453012863294 68 7 -0.879471524368256 68 8 -0.9070695750074407 68 9 -0.8205997731663146 68 10-11 -0.807064170342052 68 12-13 -0.6283568677112825 68 14-15 -0.8044653667131669 68 16-17 -0.7789670967815283 68 18-19 -0.8422532098629603 68 20-21 -0.9058061932457946 68 22-23 -1.1193867757565528 68 24-25 -0.9509792307893932 68 26-27 -1.1113988000663397 68 28-29 -1.0598367415018828 68 30-31 -1.062016485987236 68 32-33 -0.959049202158468 68 34-35 -1.0512935191121713 68 36-37 -1.0103367967359063 68 38-39 -1.0474544039167384 68 40-41 -1.0871094961933352 68 42-43 -1.0967625237566594 68 44-45 -1.0921718835577394 68 46-47 -0.8978529866036524 68 48-49 -0.9833780630199129 68 50-51 -1.194557954907829 68 52-53 -0.9865043679736587 68 54-55 -0.9475522670929308 68 56-57 -0.9615998426810606 68 58-59 -1.228472848149302 68 60-61 -1.0298618992637678 68 62-63 -1.1202734819013784 68 64-65 -0.815242497803851 68 66-67 -0.7330808102361317 68 68-69 -1.0340955814315897 68 70-71 -0.7141573360010227 68 72-73 -0.6855017042687379 68 74-75 -0.5664528951475525 68 76-77 -0.4494797088303706 68 78-79 -0.23285330852211317 68 80-81 -0.12099258376646205 68 82-83 0.21192784502889594 68 84-85 0.5468481630337862 68 86-87 1.2963977292814306 68 88-89 1.30526716021037 68 90-91 1.640109142646505 68 92-93 1.7342172453675495 68 94-95 1.8181519616305835 68 96-97 1.9354548095666964 68 98-99 1.8836428925686395 68 100-101 1.5852580192716328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 282990.0 3 35362.0 4 65077.0 5 86554.0 6 137422.0 7 187607.0 8 248772.0 9 282768.0 10 330254.0 11 370381.0 12 445224.0 13 450001.0 14 537001.0 15 595990.0 16 695420.0 17 802552.0 18 920470.0 19 1080636.0 20 1251631.0 21 1474455.0 22 1713246.0 23 2030376.0 24 2352391.0 25 2688748.0 26 3054039.0 27 3473281.0 28 4065863.0 29 4794299.0 30 5490420.0 31 6157928.0 32 6678452.0 33 7207254.0 34 7974164.0 35 9688420.0 36 1.2053818E7 37 1.3122614E7 38 7387029.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72360866478311 16.078841675846967 10.294866856470817 32.90268280289911 2 23.264894388844446 23.84584709030195 35.42079286504049 17.46846565581311 3 26.39631006637506 27.155123930058622 21.40530769479309 25.043258308773225 4 28.83821707670779 32.91418166634351 20.41862964717479 17.828971609773916 5 24.054110608129804 33.02550500976195 26.296536162618473 16.62384821948978 6 20.21653848085771 38.12126105569031 23.80828045871352 17.853920004738463 7 16.66700071700298 21.379231807200554 41.53241510290657 20.421352372889892 8 21.59821405312875 25.407378549458304 30.952772510523246 22.041634886889703 9 20.823166385076902 27.306908297357595 29.053205199669357 22.816720117896146 10-11 24.026269204291463 35.25014229047225 20.71205056691578 20.011537938320505 12-13 21.9325171521057 29.059560634182134 28.327647509996268 20.680274703715902 14-15 22.32877799452441 29.906684846810762 27.146115893704998 20.61842126495983 16-17 23.32522776405008 30.12824237836868 25.940970914163685 20.605558943417556 18-19 23.24072072813307 30.83348314182843 25.846545629024238 20.079250501014258 20-21 23.507420830682744 30.527750867999327 25.54327722216189 20.42155107915604 22-23 22.91717430144381 30.90434506274041 25.690816171107905 20.487664464707866 24-25 22.81479362809662 30.22490104651217 25.94778280742039 21.012522517970822 26-27 22.674556432207094 30.23842918663806 25.836320227614983 21.250694153539865 28-29 22.466188507715497 31.21790245406764 25.225725957599256 21.090183080617603 30-31 22.191618459600893 30.85703503702154 25.86418959138766 21.08715691198991 32-33 22.843474017774717 31.300613493926623 24.611748231446782 21.24416425685188 34-35 23.527391581657245 31.70393480211937 24.22940723256381 20.539266383659573 36-37 24.202317933592973 31.300085798195028 24.171753849237952 20.325842418974045 38-39 25.254732595943974 32.12612818685112 23.058277828306547 19.560861388898367 40-41 25.832130244406653 31.630366639877856 22.687916807331884 19.849586308383603 42-43 27.4147718566589 31.450914418860553 22.056484812066735 19.077828912413818 44-45 27.364309718851278 31.98105635137305 21.545867709486927 19.108766220288746 46-47 28.11749082808358 31.48138390100729 21.386941848422836 19.01418342248629 48-49 28.792896213918574 31.00773602440731 20.282831575455837 19.916536186218277 50-51 29.690593376856107 30.908389453382018 20.459991151040953 18.941026018720926 52-53 29.943339688862107 31.05129423444557 20.326278278464912 18.67908779822741 54-55 30.597424132761997 30.495448248185802 19.64005078760699 19.26707683144521 56-57 30.985938214780383 30.758060723188713 18.944494431981987 19.311506630048918 58-59 31.68927508200484 30.09554393774172 18.886661452722358 19.32851952753108 60-61 32.1142718070601 30.481434781240974 17.6885640534592 19.715729358239724 62-63 31.87531714236676 30.37093720689113 17.63798625970772 20.115759391034395 64-65 32.10318571812079 30.1666381083536 17.455289484184604 20.27488668934101 66-67 32.014553196827364 30.247606914878112 16.360280316220578 21.377559572073952 68-69 31.963300797856654 29.59292743247494 16.724364483146267 21.719407286522134 70-71 31.734687767056062 29.609087936457524 16.829321340381902 21.82690295610451 72-73 31.127938394086303 29.792278353011074 17.03481295899061 22.044970293912012 74-75 30.87786306329139 28.750304040051816 17.636978351223203 22.734854545433596 76-77 30.494378140331047 28.39790644097679 17.83239013288037 23.27532528581179 78-79 29.7755438572562 27.33435099212246 19.231406881656437 23.658698268964905 80-81 29.287999075388964 26.626835580692422 20.253243311363715 23.8319220325549 82-83 28.598977826619716 25.82171283531236 21.145930065270974 24.433379272796955 84-85 28.17177160112282 24.433732686682216 22.215062065575424 25.179433646619543 86-87 27.344832570235862 23.244392106261444 23.993416237598346 25.417359085904344 88-89 26.614356430053483 22.583830455336656 25.335359408296394 25.46645370631347 90-91 25.497452945538363 22.323308565257978 26.647775397258073 25.53146309194559 92-93 24.776151160256447 22.66209047410703 27.539080498150003 25.022677867486525 94-95 23.59845885113531 23.46762886254819 28.374099144768305 24.559813141548194 96-97 22.49744157664027 24.652552923881434 28.644294034030565 24.20571146544773 98-99 21.427014755697538 26.890740942216613 28.321520696474465 23.360723605611383 100-101 19.901138848343056 29.678448422372682 28.68835111039525 21.732061618889016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 91.5 2 52.0 3 52.5 4 60.5 5 71.5 6 99.0 7 157.0 8 213.0 9 272.5 10 380.5 11 534.5 12 738.0 13 981.0 14 1283.0 15 1712.0 16 2316.0 17 2983.0 18 3881.5 19 5168.0 20 7077.5 21 9958.5 22 13928.5 23 20159.5 24 28737.5 25 39653.5 26 53259.5 27 71594.0 28 99821.0 29 144006.0 30 210784.5 31 295034.0 32 444059.0 33 588820.0 34 662861.5 35 795664.0 36 996672.5 37 1260407.0 38 1625168.0 39 1808710.0 40 2006121.5 41 2466618.5 42 2969033.0 43 3510311.5 44 4291987.0 45 5342100.0 46 6425149.0 47 7264491.5 48 7773405.5 49 7965677.0 50 7796061.5 51 7385384.5 52 6835804.5 53 6187803.0 54 5426182.5 55 4566153.0 56 3663771.5 57 2788436.0 58 2028043.5 59 1425732.5 60 975497.0 61 662021.5 62 447389.0 63 290873.5 64 181579.0 65 114565.5 66 73725.5 67 48549.0 68 32601.5 69 21736.0 70 14523.5 71 9788.5 72 6622.0 73 4726.0 74 3471.0 75 2415.0 76 1720.5 77 1310.5 78 1013.0 79 771.0 80 587.5 81 431.0 82 319.5 83 238.5 84 156.0 85 111.5 86 79.0 87 52.0 88 41.5 89 24.0 90 15.0 91 15.5 92 11.5 93 13.0 94 18.0 95 12.0 96 10.5 97 13.5 98 14.5 99 23.5 100 52.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43694963524742847 2 0.0 3 0.0 4 9.073345486111789E-7 5 2.8036637552085426E-4 6 2.7220036458335363E-6 7 0.0 8 1.9961360069445933E-5 9 0.0 10-11 0.0 12-13 7.322189807292213E-4 14-15 1.4971020052084452E-5 16-17 2.4044365538196238E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006912981925868572 26-27 0.015124813258074046 28-29 0.009423576621875703 30-31 2.4180465720487915E-4 32-33 9.43627930555626E-5 34-35 1.3247084409723212E-4 36-37 2.1730662439237733E-4 38-39 6.269681730903246E-4 40-41 0.001350113808333434 42-43 0.0012480386716146766 44-45 2.4135098993057356E-4 46-47 4.083005468750305E-6 48-49 3.4297245937502563E-4 50-51 0.012274875440886334 52-53 0.023763545495401082 54-55 0.02056337654244945 56-57 0.028653171377866722 58-59 0.004771218723871884 60-61 8.637824902778423E-4 62-63 0.001973452643229314 64-65 0.0025010676832467145 66-67 0.0031470898818578737 68-69 0.004515350381163532 70-71 0.0055224917301219395 72-73 0.004658709239844097 74-75 0.0011795349131945325 76-77 0.004540302081250339 78-79 0.04442309950000332 80-81 0.051118774801479513 82-83 0.06464259624852912 84-85 0.060215257318580885 86-87 0.0052026563017364994 88-89 0.0016390998620660947 90-91 0.0015451907362848375 92-93 0.0017702097043404098 94-95 0.004553004764930896 96-97 0.00224746767690989 98-99 0.002777804720573124 100-101 0.002361338162760593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.10212931E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.747521681572586 #Duplication Level Percentage of deduplicated Percentage of total 1 64.83961093219736 15.397800664370878 2 15.804117262885548 7.5061723471698025 3 5.725376525321735 4.078905095107339 4 3.082300420145205 2.927879842260742 5 1.8779189938444427 2.229796101127894 6 1.3297304055700254 1.8946680982152293 7 0.9657658172011694 1.6054181278314508 8 0.707059549509967 1.3432729585720709 9 0.6051707559941819 1.2934175084122959 >10 4.188304126991214 19.69740459967129 >50 0.4929347346429219 8.113258007033352 >100 0.33770333120960794 15.406129200022287 >500 0.027719704774706443 4.485224170192904 >1k 0.014427672543333873 6.368182321177099 >5k 0.0010665949453493814 1.7987071585832928 >10k+ 7.931722236166455E-4 5.8537638002520715 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 504357 0.4576205309338883 No Hit CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG 382539 0.34709085089117175 Illumina Paired End PCR Primer 2 (96% over 33bp) GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 326071 0.2958554836001957 No Hit CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 302028 0.2740404390479371 Illumina Paired End PCR Primer 2 (100% over 31bp) CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 161170 0.14623510919966368 No Hit CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA 160432 0.14556549630278864 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 115870 0.10513285414757728 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.7220036458335364E-5 3.6293381944447156E-6 0.0 0.0 0.0 2 6.532808750000488E-5 6.351341840278252E-6 0.0 0.0 0.0 3 1.2249016406250916E-4 7.258676388889431E-6 0.0 0.0 9.073345486111789E-7 4 1.8509624791668048E-4 7.258676388889431E-6 0.0 0.0 1.8146690972223578E-6 5 2.504243354166854E-4 8.16601093750061E-6 0.0 0.0 1.8146690972223578E-6 6 3.039570737847449E-4 9.980680034722967E-6 0.0 0.0 1.8146690972223578E-6 7 3.6565582309030506E-4 1.1795349131945325E-5 0.0 5.444007291667073E-6 1.8146690972223578E-6 8 4.1918856145836464E-4 1.1795349131945325E-5 0.0 6.351341840278252E-6 1.8146690972223578E-6 9 4.8814598715281424E-4 1.1795349131945325E-5 0.0 8.16601093750061E-6 1.8146690972223578E-6 10-11 0.00104298106362855 1.2249016406250914E-5 0.0 8.619678211806199E-6 1.8146690972223578E-6 12-13 0.0014662526305556651 1.4063685503473271E-5 0.0 9.527012760417377E-6 1.8146690972223578E-6 14-15 0.006192558294271296 1.8600358246529167E-5 0.0 1.1795349131945325E-5 1.8146690972223578E-6 16-17 0.011372531232292517 3.1756709201391257E-5 0.0 1.4063685503473271E-5 1.8146690972223578E-6 18-19 0.014120847580035775 4.355205833333659E-5 9.073345486111789E-7 2.0868694618057115E-5 1.8146690972223578E-6 20-21 0.3223156273740692 4.763506380208689E-5 9.073345486111789E-7 2.449803281250183E-5 1.8146690972223578E-6 22-23 0.3500977575852692 4.990340017361484E-5 9.073345486111789E-7 2.6766369184029777E-5 2.7220036458335367E-6 24-25 0.4176374730475138 4.990340017361484E-5 9.073345486111789E-7 3.039570737847449E-5 2.7220036458335367E-6 26-27 0.7019888619058683 5.081073472222602E-5 9.073345486111789E-7 3.4478712847224795E-5 2.7220036458335367E-6 28-29 1.852117516047187 5.6254742013893085E-5 9.073345486111789E-7 3.674704921875274E-5 2.7220036458335367E-6 30-31 3.2031495469438154 5.9884080208337806E-5 9.073345486111789E-7 4.582039470486453E-5 2.7220036458335367E-6 32-33 5.234895712917751 6.079141475694898E-5 9.073345486111789E-7 5.1718069270837196E-5 3.6293381944447156E-6 34-35 7.810236441311954 6.623542204861606E-5 9.073345486111789E-7 5.6254742013893085E-5 4.083005468750305E-6 36-37 11.158357634096493 6.8503758420144E-5 9.073345486111789E-7 5.806941111111545E-5 4.536672743055894E-6 38-39 15.023467618332372 6.986476024306077E-5 9.073345486111789E-7 5.8976745659726624E-5 4.536672743055894E-6 40-41 19.09789786826375 7.077209479167195E-5 9.073345486111789E-7 5.8976745659726624E-5 4.536672743055894E-6 42-43 23.261766806655384 7.621610208333902E-5 9.073345486111789E-7 5.9430412934032215E-5 4.536672743055894E-6 44-45 27.465295338166808 8.846511848958994E-5 9.073345486111789E-7 6.169874930556016E-5 4.536672743055894E-6 46-47 31.710950051768425 1.3428551319445448E-4 9.073345486111789E-7 6.169874930556016E-5 4.536672743055894E-6 48-49 35.851138465775854 1.3564651501737123E-4 9.073345486111789E-7 6.169874930556016E-5 4.536672743055894E-6 50-51 39.89442763299708 1.37007516840288E-4 9.073345486111789E-7 6.215241657986576E-5 4.536672743055894E-6 52-53 43.715283735626265 1.3791485138889918E-4 9.073345486111789E-7 6.305975112847693E-5 4.990340017361483E-6 54-55 47.32406581220492 1.3791485138889918E-4 9.073345486111789E-7 6.44207529513937E-5 5.444007291667073E-6 56-57 50.76876868468365 1.3791485138889918E-4 9.073345486111789E-7 6.532808750000488E-5 5.444007291667073E-6 58-59 53.997291388612105 1.3836851866320477E-4 9.073345486111789E-7 7.122576206597754E-5 5.444007291667073E-6 60-61 57.05838546295443 1.3882218593751038E-4 9.073345486111789E-7 7.167942934028312E-5 5.444007291667073E-6 62-63 59.96054854942565 1.3927585321181596E-4 9.073345486111789E-7 7.167942934028312E-5 5.444007291667073E-6 64-65 62.62932159929582 1.415441895833439E-4 9.073345486111789E-7 7.25867638888943E-5 5.444007291667073E-6 66-67 65.12777933471345 1.4381252595487183E-4 9.073345486111789E-7 7.349409843750549E-5 5.444007291667073E-6 68-69 67.51620279475192 1.4562719505209422E-4 9.073345486111789E-7 7.349409843750549E-5 5.444007291667073E-6 70-71 69.68903131702396 1.4880286597223334E-4 9.073345486111789E-7 7.440143298611667E-5 6.351341840278252E-6 72-73 71.69493069737888 1.506175350694557E-4 9.073345486111789E-7 7.71234366319502E-5 6.351341840278252E-6 74-75 73.5850528283292 1.5243220416667806E-4 9.073345486111789E-7 7.71234366319502E-5 6.351341840278252E-6 76-77 75.2937729239775 1.5333953871528923E-4 9.073345486111789E-7 7.71234366319502E-5 6.351341840278252E-6 78-79 76.93109304932649 1.5333953871528923E-4 9.073345486111789E-7 7.71234366319502E-5 6.351341840278252E-6 80-81 78.43762498249865 1.5333953871528923E-4 9.073345486111789E-7 7.803077118056138E-5 7.258676388889431E-6 82-83 79.7843394619457 1.5333953871528923E-4 9.073345486111789E-7 7.893810572917256E-5 7.258676388889431E-6 84-85 81.0415698862051 1.5333953871528923E-4 9.073345486111789E-7 8.483578029514523E-5 7.71234366319502E-6 86-87 82.17393474455369 1.5333953871528923E-4 9.073345486111789E-7 8.710411666667316E-5 8.16601093750061E-6 88-89 83.22326261334979 1.5333953871528923E-4 9.073345486111789E-7 8.98261203125067E-5 8.16601093750061E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGGAT 15915 0.0 36.50223 1 CGCGCGT 3075 0.0 36.295227 1 GCGCGTC 4320 0.0 32.098145 2 GTTTATT 87265 0.0 29.159033 1 GCGGATT 18530 0.0 28.699759 1 GCGAATT 6745 0.0 27.598465 2 CGGGCAT 12620 0.0 24.565907 1 CGGGGCT 17735 0.0 24.526886 1 CGGCGGT 3700 0.0 24.363453 1 CGGATTT 20285 0.0 23.948671 2 GGCGAAT 9515 0.0 23.81023 1 CTTCGAC 6005 0.0 23.40804 8 CCCGCGT 6880 0.0 23.362549 1 CGGGCGT 4860 0.0 22.768293 1 CCCGGGT 7205 0.0 22.639711 1 CGAATTT 7995 0.0 21.442211 3 CGGGGAT 10345 0.0 21.162207 1 GTCGGGT 11190 0.0 21.141235 1 GGAGAAT 124270 0.0 20.986546 1 CGGGTGT 12915 0.0 20.7549 1 >>END_MODULE