FastQCFastQC Report
Fri 27 May 2016
SRR1233079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115613493
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT224500.066.3024751
ACGCGTC280200.048.6190452
CGCGTGT1271700.047.801161
CACGCGT290850.046.588611
GCGTGTT1399850.043.2452772
CGCGCGT51400.041.7659531
CGCGTCT508400.035.0217173
CGTGTTT1781800.034.1857833
CCCGCTC300900.032.8077432
CCCCGCT302100.032.3550221
CGGGCGT95700.030.968471
CCAACGA102800.030.7616338
CCGTACT93850.030.3571532
GGCATTC491100.030.3408012
GCGTCTC599650.029.890224
CAACGAA113250.028.5101159
CCCGTAC92700.027.8718781
TGAACCG218050.027.24096712-13
CGGGTGT163400.026.741321
CCGGAAT109050.026.6546421
Produced by FastQC (version 0.11.5)