##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115613493 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.004079437336955 39.0 37.0 39.0 33.0 39.0 2 36.74515650175884 39.0 37.0 39.0 32.0 39.0 3 36.665539289605235 39.0 37.0 39.0 32.0 39.0 4 36.59027374079944 39.0 36.0 39.0 32.0 39.0 5 36.573689647107194 39.0 36.0 39.0 32.0 39.0 6 36.64451490104187 39.0 37.0 39.0 33.0 39.0 7 36.68414658140291 39.0 37.0 39.0 33.0 39.0 8 36.58210031765064 39.0 36.0 39.0 32.0 39.0 9 36.557408649525016 39.0 36.0 39.0 32.0 39.0 10-11 36.50877674805656 39.0 36.0 39.0 31.5 39.0 12-13 36.422903752246285 39.0 36.0 39.0 31.5 39.0 14-15 36.34794481990091 39.0 36.0 39.0 31.0 39.0 16-17 36.21561679223721 39.0 36.0 39.0 31.0 39.0 18-19 36.08567913435502 39.0 36.0 39.0 31.0 39.0 20-21 35.964563906913526 38.5 36.0 39.0 31.0 39.0 22-23 35.74909807888946 38.0 36.0 39.0 30.0 39.0 24-25 35.64574832973864 38.0 36.0 39.0 30.0 39.0 26-27 35.4510203060814 38.0 35.5 39.0 29.0 39.0 28-29 35.248713461152846 38.0 35.0 39.0 28.5 39.0 30-31 35.35398860840577 38.0 35.5 39.0 29.0 39.0 32-33 35.10014935280954 38.0 35.0 39.0 28.0 39.0 34-35 35.01714811522908 38.0 35.0 39.0 28.0 39.0 36-37 34.95238754268933 38.0 35.0 39.0 28.0 39.0 38-39 34.768543633570516 38.0 35.0 39.0 27.0 39.0 40-41 34.64572165897626 37.5 35.0 39.0 27.0 39.0 42-43 34.5054765796238 37.0 34.0 39.0 27.0 39.0 44-45 34.35886314324921 37.0 34.0 39.0 26.0 39.0 46-47 34.14435400286712 37.0 34.0 39.0 26.0 39.0 48-49 33.86050488933848 37.0 33.5 39.0 25.0 39.0 50-51 33.94460215815813 37.5 34.0 39.0 25.0 39.0 52-53 34.165963262609836 38.0 34.0 39.0 25.0 39.0 54-55 34.10915731955266 37.5 34.0 39.0 25.0 39.0 56-57 34.14936021351764 38.0 34.5 39.0 25.0 39.0 58-59 33.96676571738906 37.5 34.0 39.0 24.5 39.0 60-61 33.7300119070012 37.0 34.0 39.0 23.5 39.0 62-63 33.431082412673064 37.0 33.0 39.0 22.0 39.0 64-65 33.23435614474515 37.0 33.0 39.0 22.0 39.0 66-67 32.970403874053005 37.0 33.0 39.0 20.0 39.0 68-69 32.7120597766214 37.0 33.0 39.0 20.0 39.0 70-71 32.43854376495657 37.0 32.0 39.0 17.5 39.0 72-73 32.082870461322365 36.0 32.0 39.0 16.5 39.0 74-75 31.717804054237856 36.0 31.0 38.5 14.0 39.0 76-77 31.111744418101786 36.0 30.5 38.0 4.0 39.0 78-79 30.588728073461112 35.0 29.5 37.0 2.0 39.0 80-81 30.155945815943817 35.0 29.0 37.0 2.0 39.0 82-83 29.611108112614502 34.5 28.0 37.0 2.0 39.0 84-85 29.071598904982483 34.0 27.0 37.0 2.0 39.0 86-87 28.33822379624842 34.0 26.0 37.0 2.0 39.0 88-89 27.646871243653194 33.0 24.5 36.0 2.0 38.5 90-91 26.60669429821656 33.0 21.5 36.0 2.0 38.0 92-93 25.526205985316956 32.0 18.0 36.0 2.0 37.0 94-95 24.46989066838418 31.0 7.5 35.5 2.0 37.0 96-97 22.789426105307623 30.5 2.0 35.0 2.0 37.0 98-99 21.709707512253782 29.0 2.0 34.5 2.0 37.0 100-101 20.714932516570535 27.5 2.0 34.5 2.0 36.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.5301326344897106 1 2 0.6646931009780417 1 3 0.6657338846349887 1 4 0.6847643171744835 1 5 0.6624297272197808 1 6 0.6406628165770911 1 7 0.6269658522513097 1 8 0.6258572283245059 1 9 0.6260485498645707 1 10-11 0.5941262934772951 1 12-13 0.6065136090210288 1 14-15 0.608352229990011 1 16-17 0.6216649990801315 1 18-19 0.6422991398355506 1 20-21 0.644976395342816 1 22-23 0.6159938070601072 1 24-25 0.6139439207703035 1 26-27 0.6505395076853091 1 28-29 0.6259649299258854 1 30-31 0.7129247203324027 1 32-33 0.8670262675330704 1 34-35 0.79042728767363 1 36-37 0.8570173997706192 1 38-39 0.8171728207878388 1 40-41 0.837798196751848 1 42-43 0.7856029199692216 1 44-45 0.7854260121538914 1 46-47 -0.1895215181064387 1 48-49 0.40416979188917423 1 50-51 0.5396666746985446 1 52-53 0.6074064829615651 1 54-55 0.6306394088118026 1 56-57 0.7749525790637932 1 58-59 0.7261792160915661 1 60-61 0.7315050026182703 1 62-63 0.7269053196761277 1 64-65 0.6328087825287554 1 66-67 0.6175301244884821 1 68-69 0.6082797580025954 1 70-71 0.5564962643482758 1 72-73 0.5058576676984714 1 74-75 0.4939490999266809 1 76-77 0.2971281919603541 1 78-79 0.16229085242202146 1 80-81 0.029250724685454088 1 82-83 -0.10938252346671717 1 84-85 -0.21786503272075208 1 86-87 -0.521158934216043 1 88-89 -0.6076779357366924 1 90-91 -0.8802106596784931 1 92-93 -1.1519207727021978 1 94-95 -1.2748552620121671 1 96-97 -1.5695766882238011 1 98-99 -1.6551020817302877 1 100-101 -1.6181224598179362 2 1 0.42309635909360566 2 2 0.5411911985278195 2 3 0.5616886608339087 2 4 0.5566755202870368 2 5 0.5104353838674172 2 6 0.5474326771399092 2 7 0.5236652638646646 2 8 0.5389813086016417 2 9 0.5005035428996294 2 10-11 0.5341610299388933 2 12-13 0.573261594182064 2 14-15 0.5708021572341977 2 16-17 0.6012479264677992 2 18-19 0.57658954231065 2 20-21 0.5863729827748401 2 22-23 0.5836194652021547 2 24-25 0.5604296513841334 2 26-27 0.49318445918743237 2 28-29 0.5395225172816964 2 30-31 0.5766459450348762 2 32-33 0.6880065376487181 2 34-35 0.6767614591405859 2 36-37 0.732185226888717 2 38-39 0.7642522632975002 2 40-41 0.7679026876433355 2 42-43 0.6829910568307582 2 44-45 0.7206811120687249 2 46-47 0.5737772193392416 2 48-49 0.6250129111717513 2 50-51 0.6166863074156836 2 52-53 0.6294169245264243 2 54-55 0.6107573054529212 2 56-57 0.7059762394736708 2 58-59 0.6899772240367525 2 60-61 0.6600253848941477 2 62-63 0.66426095254252 2 64-65 0.680128266236629 2 66-67 0.6247919024518254 2 68-69 0.5226346331966312 2 70-71 0.5429361299753239 2 72-73 0.5688638174292393 2 74-75 0.5656170808923804 2 76-77 0.48563074994286737 2 78-79 0.4050712235256704 2 80-81 0.3544388967169141 2 82-83 0.24761832750718327 2 84-85 0.23752015758608636 2 86-87 0.12610240429590647 2 88-89 0.068073935521344 2 90-91 -0.1042085082702009 2 92-93 -0.2909972893780868 2 94-95 -0.432175446272403 2 96-97 -0.6262610282240075 2 98-99 -0.7228747536783153 2 100-101 -0.7578618851081096 3 1 0.4036591787936956 3 2 0.504844783803371 3 3 0.5193031620635011 3 4 0.4935464082657006 3 5 0.4912791294955312 3 6 0.4701902334813681 3 7 0.4717369428087679 3 8 0.46541195556977044 3 9 0.4081051533710678 3 10-11 0.4255422570089138 3 12-13 0.38612901311648073 3 14-15 0.3998862946306332 3 16-17 0.4059142752705398 3 18-19 0.3838619623070514 3 20-21 0.46181471453593304 3 22-23 0.4896334442473247 3 24-25 0.46018928281245763 3 26-27 0.47048815598720495 3 28-29 0.4473399484952125 3 30-31 0.4581529348262521 3 32-33 0.5251550034859278 3 34-35 0.5214798058504044 3 36-37 0.5036127142203952 3 38-39 0.6002872932657297 3 40-41 0.5325745479393191 3 42-43 0.5940438523052478 3 44-45 0.6173081568348593 3 46-47 0.5351428775166056 3 48-49 0.5362151257604921 3 50-51 0.6026286618487333 3 52-53 0.6023666655657891 3 54-55 0.5220432222163609 3 56-57 0.6314619988179189 3 58-59 0.5740071412223031 3 60-61 0.5732854265895 3 62-63 0.660209072667449 3 64-65 0.6120713050460722 3 66-67 0.6336142907070936 3 68-69 0.6443392626724886 3 70-71 0.6156988398420324 3 72-73 0.6296071917901429 3 74-75 0.6254994360323778 3 76-77 0.5709967432726302 3 78-79 0.5617478006366241 3 80-81 0.562408541131802 3 82-83 0.524305314515626 3 84-85 0.4535894372029645 3 86-87 0.33755916197032576 3 88-89 0.4130027470004869 3 90-91 0.4079370679770733 3 92-93 0.3646204470519798 3 94-95 0.25399807409284136 3 96-97 0.05046979126829143 3 98-99 -0.09162370141111253 3 100-101 -0.0629872970400065 4 1 0.34130465511837826 4 2 0.39226117117424764 4 3 0.39768217199767975 4 4 0.3911414427349271 4 5 0.3514366018812467 4 6 0.3650493771885195 4 7 0.4042738811633413 4 8 0.371338710016893 4 9 0.41073610797712945 4 10-11 0.3689108814024493 4 12-13 0.3982133430497541 4 14-15 0.40683662690253186 4 16-17 0.38226279567009414 4 18-19 0.4057341715451699 4 20-21 0.4233053943327789 4 22-23 0.43453478295544556 4 24-25 0.42448366434756934 4 26-27 0.405558012015355 4 28-29 0.44112084141661967 4 30-31 0.46995944793903277 4 32-33 0.5997793725989311 4 34-35 0.5011396450040237 4 36-37 0.6275401814243864 4 38-39 0.610308268163422 4 40-41 0.6674648297975878 4 42-43 0.6647965293420128 4 44-45 0.6098770149357762 4 46-47 0.4820208893101636 4 48-49 0.5330411079483639 4 50-51 0.591407467442501 4 52-53 0.5611213308676071 4 54-55 0.5208759574274353 4 56-57 0.6345155858057154 4 58-59 0.5503771132666344 4 60-61 0.6220422989113885 4 62-63 0.6416209515576696 4 64-65 0.592044688415065 4 66-67 0.6467493973482874 4 68-69 0.6596030624164797 4 70-71 0.6538258431312514 4 72-73 0.6795372492744889 4 74-75 0.666096133748816 4 76-77 0.7334280484312501 4 78-79 0.7343151575852715 4 80-81 0.6856605971875496 4 82-83 0.726655610164773 4 84-85 0.8045223593653539 4 86-87 0.8125020577034014 4 88-89 0.9176353407891007 4 90-91 0.8741290413751805 4 92-93 0.8483225540446355 4 94-95 0.82899256783422 4 96-97 0.6806427866522782 4 98-99 0.6354968598453254 4 100-101 0.5892473645846614 5 1 0.25040454782271837 5 2 0.2585454995016647 5 3 0.2623955724342366 5 4 0.2752165011828893 5 5 0.27041699295949684 5 6 0.2531872608481791 5 7 0.2693626331832135 5 8 0.2630077555703565 5 9 0.2571351026216533 5 10-11 0.24281509032179116 5 12-13 0.2708535894198789 5 14-15 0.2625353461698765 5 16-17 0.22753867398004246 5 18-19 0.24014457923886567 5 20-21 0.2678882678719958 5 22-23 0.2188826040347962 5 24-25 0.2071306504516386 5 26-27 0.25857385501220875 5 28-29 0.24132710187078033 5 30-31 0.2940844685831223 5 32-33 0.5012900308946797 5 34-35 0.40371704353876936 5 36-37 0.48708238109450974 5 38-39 0.5119337605840215 5 40-41 0.4948370648860134 5 42-43 0.4517038793819097 5 44-45 0.4612093280884011 5 46-47 0.3828190528154565 5 48-49 0.36918007865043023 5 50-51 0.39527713444181956 5 52-53 0.3981749606930123 5 54-55 0.3857010552271589 5 56-57 0.5912716135141167 5 58-59 0.5268689517207434 5 60-61 0.6233727344035316 5 62-63 0.7061038525630536 5 64-65 0.6679073887396783 5 66-67 0.6607179335500177 5 68-69 0.6770708974788917 5 70-71 0.6875054726255954 5 72-73 0.7428157270771578 5 74-75 0.6868163241310015 5 76-77 0.7270600194013355 5 78-79 0.722184169437714 5 80-81 0.7451165214482742 5 82-83 0.7612591101890551 5 84-85 0.8799921644660422 5 86-87 0.9325302033717158 5 88-89 1.0350626198602662 5 90-91 1.0559085269088477 5 92-93 1.033150885588654 5 94-95 0.9755878634273856 5 96-97 0.8167321156611003 5 98-99 0.8545318661858197 5 100-101 0.8240053608215483 6 1 0.07106284524434159 6 2 0.03439407306472475 6 3 0.05447822051088025 6 4 0.027498485812998297 6 5 0.01779088135871376 6 6 0.047230097129400406 6 7 0.06621474682761885 6 8 0.03382515891693316 6 9 0.024840261836480693 6 10-11 0.026367485490055742 6 12-13 0.05017085789507547 6 14-15 0.05704548459560499 6 16-17 0.06736011419149435 6 18-19 0.0716473368326902 6 20-21 -0.0012724293539250198 6 22-23 0.04446889230703732 6 24-25 -0.003949605926727884 6 26-27 -0.005704048670359896 6 28-29 -0.02066354650898461 6 30-31 0.046931593808025696 6 32-33 0.24410988930677036 6 34-35 0.13033788980844463 6 36-37 0.20022289444506214 6 38-39 0.24437596804887818 6 40-41 0.2790482021656615 6 42-43 0.27642332917008616 6 44-45 0.21784251744264793 6 46-47 0.13704118915262598 6 48-49 0.17492304604756725 6 50-51 0.27998167401044327 6 52-53 0.2806974653118459 6 54-55 0.24233856993188851 6 56-57 0.3947882472641311 6 58-59 0.35341526684269553 6 60-61 0.4089630260719517 6 62-63 0.4486393168666254 6 64-65 0.4106260161484627 6 66-67 0.4792288513916816 6 68-69 0.45475995872730834 6 70-71 0.48933003581684176 6 72-73 0.5360867344731872 6 74-75 0.5094942991797708 6 76-77 0.6363777979247374 6 78-79 0.675369253541831 6 80-81 0.7581309381983168 6 82-83 0.7765921834405525 6 84-85 0.9167612184051421 6 86-87 0.9748845013329905 6 88-89 1.0351176542198175 6 90-91 1.1243999184019806 6 92-93 1.1881134123588275 6 94-95 1.1781844026924766 6 96-97 1.0730951706001548 6 98-99 1.0748784436175391 6 100-101 1.100238135093928 7 1 -0.18270398576716929 7 2 -0.28008180095783786 7 3 -0.2989318924248252 7 4 -0.37840549817715896 7 5 -0.2757419334679625 7 6 -0.3017212827292113 7 7 -0.21963222669371874 7 8 -0.20105223433084518 7 9 -0.24882273064972793 7 10-11 -0.22621051539157122 7 12-13 -0.16975244803897738 7 14-15 -0.17873711579410667 7 16-17 -0.20947814840904044 7 18-19 -0.21761631132495296 7 20-21 -0.1262167241690051 7 22-23 -0.12200020298719494 7 24-25 -0.17002324944828473 7 26-27 -0.15221044310190024 7 28-29 -0.14609353370137512 7 30-31 -0.17310829915800952 7 32-33 -0.40336550737250576 7 34-35 -0.38281263543432686 7 36-37 -0.5283726538005169 7 38-39 -0.4070219852381243 7 40-41 -0.39692679758985605 7 42-43 -0.232514085801931 7 44-45 -0.22604486889524367 7 46-47 -0.039728920145471136 7 48-49 -0.019111239061814445 7 50-51 -0.10142653780629729 7 52-53 -0.15123736503423402 7 54-55 -0.13476572509558338 7 56-57 0.11177783149267384 7 58-59 0.086756031347619 7 60-61 0.011326330949877672 7 62-63 0.03625653841110932 7 64-65 -0.01922952872856598 7 66-67 0.18004535213948714 7 68-69 0.25018783810136114 7 70-71 0.21792808834275235 7 72-73 0.374175129918747 7 74-75 0.41006133492103913 7 76-77 0.36149770450220586 7 78-79 0.4461955670106441 7 80-81 0.5481528299126168 7 82-83 0.661757380174425 7 84-85 0.7206427150160586 7 86-87 0.8287409879409857 7 88-89 0.8309077193328811 7 90-91 0.934248591884927 7 92-93 1.1450543197201064 7 94-95 1.2091609554179321 7 96-97 1.2285864260098727 7 98-99 1.1910855510734741 7 100-101 0.6761220667553332 8 1 -0.9721884951945583 8 2 -1.2256118686063857 8 3 -1.3899990790572545 8 4 -1.1231407414652281 8 5 -1.0173818956428278 8 6 -1.0235705306818446 8 7 -1.0672680567263768 8 8 -1.0259026801162676 8 9 -1.166394103494099 8 10-11 -1.088330950927201 8 12-13 -1.122519725034131 8 14-15 -1.1684560670948656 8 16-17 -1.0604139522291973 8 18-19 -1.06890187599398 8 20-21 -1.1520485448376192 8 22-23 -1.2011452301997707 8 24-25 -1.0817382495009014 8 26-27 -0.9561286908594226 8 28-29 -0.9257985684809285 8 30-31 -1.2472737495504091 8 32-33 -2.203824437753184 8 34-35 -1.9284009741494756 8 36-37 -2.2086105459082717 8 38-39 -2.026416296341459 8 40-41 -2.46745162071079 8 42-43 -2.3667272249540616 8 44-45 -2.5052473294729367 8 46-47 -1.2757617995913293 8 48-49 -1.294619805330882 8 50-51 -1.321583939822645 8 52-53 -1.0728001114698174 8 54-55 -1.2320476824961872 8 56-57 -2.284079144077033 8 58-59 -1.913335181214947 8 60-61 -2.5536358968732813 8 62-63 -2.5976547510798405 8 64-65 -2.501491733510303 8 66-67 -1.9093966035670675 8 68-69 -1.4559599351049997 8 70-71 -1.5948036233032425 8 72-73 -1.169132292329202 8 74-75 -1.6191537503510105 8 76-77 -1.384796825835064 8 78-79 -1.2454587373267039 8 80-81 -0.9457532810968097 8 82-83 -0.8881292527811695 8 84-85 -0.8410416422388174 8 86-87 -0.4568293627642106 8 88-89 -0.12968062988156603 8 90-91 0.3457468929639269 8 92-93 0.33583388637724454 8 94-95 0.7021560243518614 8 96-97 1.3311820179695744 8 98-99 1.409871641523953 8 100-101 1.4323576980013186 21 1 0.4178095036140377 21 2 0.5371708957960166 21 3 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0.28702429745708713 65 66-67 0.3295046609545409 65 68-69 0.2768929145998982 65 70-71 0.30798397325022364 65 72-73 0.19289470445156098 65 74-75 0.12901635329170347 65 76-77 0.12361812744562428 65 78-79 0.13488181994909 65 80-81 0.14882686618184238 65 82-83 0.31140728892998126 65 84-85 0.2873592960313225 65 86-87 0.3640194417010285 65 88-89 0.3994121713412575 65 90-91 0.4377092842812047 65 92-93 0.3812369432316238 65 94-95 0.3862147451337492 65 96-97 0.408686039168888 65 98-99 0.36764364992593457 65 100-101 0.3801656418486026 66 1 -0.097118237962448 66 2 -0.11904860013549268 66 3 -0.12414868072987417 66 4 -0.12234092130007213 66 5 -0.12848218597176242 66 6 -0.1343957315921358 66 7 -0.15186617199735508 66 8 -0.21397225563753608 66 9 -0.17460745067081263 66 10-11 -0.1872256405376831 66 12-13 -0.19410110505837253 66 14-15 -0.17267529382212388 66 16-17 -0.1462328480551207 66 18-19 -0.14163509744224 66 20-21 -0.18207919574232534 66 22-23 -0.17452887479672086 66 24-25 -0.17418558294413344 66 26-27 -0.22899790419590005 66 28-29 -0.21840899096318367 66 30-31 -0.11907939571971582 66 32-33 0.06552794660177597 66 34-35 -0.03850496664563963 66 36-37 0.1070877006808928 66 38-39 0.09836821419529684 66 40-41 0.13480379742179593 66 42-43 0.1154313318265281 66 44-45 0.140229617288135 66 46-47 -0.050241315589062197 66 48-49 -0.09599277446223198 66 50-51 -0.0986243135627447 66 52-53 -0.05156999687176267 66 54-55 -0.04854073368501588 66 56-57 0.14339102189774167 66 58-59 0.12512943758923 66 60-61 0.18123570394467237 66 62-63 0.18270001759285748 66 64-65 0.13162113873533343 66 66-67 0.1702824860856822 66 68-69 0.1909644954989531 66 70-71 0.2559230043352727 66 72-73 0.24080014834349583 66 74-75 0.2751751728496963 66 76-77 0.35413882461224944 66 78-79 0.3478261530138731 66 80-81 0.3839240671378228 66 82-83 0.40803733815186405 66 84-85 0.4198456033414253 66 86-87 0.5431300566915702 66 88-89 0.5080728179254486 66 90-91 0.6745598560263524 66 92-93 0.7136180670271521 66 94-95 0.7361174221306257 66 96-97 0.8696049002748296 66 98-99 0.811504578313258 66 100-101 0.8478094472848703 67 1 -0.44866225313788277 67 2 -0.5883260243438571 67 3 -0.5203674672083949 67 4 -0.748443444683133 67 5 -0.7101656707586486 67 6 -0.6123378577120988 67 7 -0.6167291092477711 67 8 -0.5560571346758181 67 9 -0.5733740145102786 67 10-11 -0.5635414711869942 67 12-13 -0.4929217937207824 67 14-15 -0.48161578305573016 67 16-17 -0.5264845703252448 67 18-19 -0.49063836813697037 67 20-21 -0.6020455075070359 67 22-23 -0.5499611589100795 67 24-25 -0.5974261098181799 67 26-27 -0.5235502927746083 67 28-29 -0.4639727551171191 67 30-31 -0.520158954888835 67 32-33 -0.3953002100318628 67 34-35 -0.47365345945200943 67 36-37 -0.46231562940857884 67 38-39 -0.6000953262792521 67 40-41 -0.5745603228796767 67 42-43 -0.5363210856243583 67 44-45 -0.4214701900096003 67 46-47 -0.3406586371098541 67 48-49 -0.43558621913642526 67 50-51 -0.5975014447864879 67 52-53 -0.5427997088157781 67 54-55 -0.6006098637261772 67 56-57 -0.5817136333537505 67 58-59 -0.5511200547460788 67 60-61 -0.5348919033811157 67 62-63 -0.5210232099435572 67 64-65 -0.5868611581736474 67 66-67 -0.6026908640118052 67 68-69 -0.587247372645848 67 70-71 -0.49711228709832866 67 72-73 -0.5749082020232059 67 74-75 -0.3648026245713538 67 76-77 -0.277890082535869 67 78-79 -0.31168336172895295 67 80-81 -0.356576094190892 67 82-83 0.16956874952517254 67 84-85 0.05501598517576767 67 86-87 0.30063609173768313 67 88-89 0.37752422690195786 67 90-91 0.358621036240951 67 92-93 0.6546187052643226 67 94-95 0.8068985249607508 67 96-97 1.0398416403962862 67 98-99 1.0520812566277336 67 100-101 1.068662927884656 68 1 -1.4168227615592883 68 2 -1.6077284807398797 68 3 -1.8004735660790985 68 4 -1.6110857890446653 68 5 -1.5605550715863288 68 6 -1.6268996278297863 68 7 -1.6479206411391658 68 8 -1.8905118093506559 68 9 -1.6525035341665628 68 10-11 -1.6032807572516177 68 12-13 -1.5984674664464293 68 14-15 -1.6314875882768547 68 16-17 -1.4932347670724724 68 18-19 -1.5770771616643557 68 20-21 -1.51315931488152 68 22-23 -1.4873898478598733 68 24-25 -1.5095413480182174 68 26-27 -1.3873817792674217 68 28-29 -1.3733413617964558 68 30-31 -1.764668594051571 68 32-33 -2.37019292894837 68 34-35 -1.8756193209661873 68 36-37 -2.380817088456432 68 38-39 -2.7107749644673262 68 40-41 -2.9446429100184552 68 42-43 -3.17065681291232 68 44-45 -3.082465953854552 68 46-47 -2.1634338976843637 68 48-49 -1.9413721962572765 68 50-51 -1.9995866912102187 68 52-53 -1.8531648229711948 68 54-55 -1.955918612908519 68 56-57 -2.453522277140806 68 58-59 -2.440330029600755 68 60-61 -2.6025301778241534 68 62-63 -2.85186034210874 68 64-65 -2.7673099217166808 68 66-67 -2.599290143470533 68 68-69 -2.5881687510161626 68 70-71 -2.804954409121134 68 72-73 -2.883390829700616 68 74-75 -2.5522295756481164 68 76-77 -1.869731959561122 68 78-79 -1.555072085384868 68 80-81 -1.550127541605626 68 82-83 -0.8862209031594155 68 84-85 -1.177466306562028 68 86-87 -0.39159500854458784 68 88-89 -0.6284420505728754 68 90-91 -0.6845267277524094 68 92-93 0.0021656888063823487 68 94-95 0.28795539936856684 68 96-97 0.9204059904375974 68 98-99 0.944739211088276 68 100-101 1.0466673722593818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 851247.0 3 111196.0 4 164333.0 5 176457.0 6 210109.0 7 246082.0 8 343942.0 9 381461.0 10 518454.0 11 511832.0 12 602431.0 13 450932.0 14 504495.0 15 497704.0 16 540101.0 17 593834.0 18 648156.0 19 740414.0 20 840456.0 21 980657.0 22 1114606.0 23 1289202.0 24 1456990.0 25 1667402.0 26 1906974.0 27 2195247.0 28 2542820.0 29 2968717.0 30 3549224.0 31 4309558.0 32 5354140.0 33 6800583.0 34 8700886.0 35 1.1281671E7 36 1.5130088E7 37 2.1274436E7 38 1.4156605E7 39 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.08788159826532 11.065858233520183 12.883338945656236 41.962921222558265 2 21.199893550988897 18.72973155564258 37.80781288537799 22.26256200799053 3 25.979971385219823 18.74166212008642 25.973297055915257 29.305069438778503 4 29.500400608797605 23.367725273210645 24.428221733119674 22.70365238487207 5 24.972288904103337 25.929276607155483 30.39521691306057 18.703217575680608 6 21.673605342730077 29.26735242740331 27.865936403260456 21.193105826606157 7 16.186123591165018 15.50072812713244 47.1974043119038 21.115743969798743 8 20.92177035595933 20.990972105866152 33.050995203126426 25.036262335048093 9 19.679093782300484 20.176990298493163 35.269273968650815 24.87464195055554 10-11 23.1861025642635 27.964847575977185 26.207528509942595 22.641521349816724 12-13 20.69826052096159 22.27710097849862 33.2830133668038 23.741625133735994 14-15 21.08711239433942 23.615955243599615 32.419761570774014 22.877170791286954 16-17 21.402906914044653 23.91780083247989 31.191640321165252 23.487651932310204 18-19 21.46996824946113 24.046265905704114 31.315571312212402 23.168194532622348 20-21 21.331704166115646 24.133563162280115 31.10283206294025 23.431900608663987 22-23 21.325963824105063 24.142247506034515 30.993093680024586 23.53869498983584 24-25 21.38435150235482 24.028782641345526 31.15246671084663 23.434399145453018 26-27 20.936326311906846 24.566152127388172 31.046296985251097 23.45122457545388 28-29 21.39083534728183 24.82475209605248 30.34112934499963 23.443283211666056 30-31 21.2402860547921 25.035343756191146 30.248003949420095 23.476366239596658 32-33 21.569347792120407 25.414190699857496 29.581470601262676 23.43499090675942 34-35 22.45943395901863 25.959100762985273 28.713499812852422 22.867965465143676 36-37 23.170798723744703 26.454106509434443 27.985959715015984 22.389135051804868 38-39 24.027540368834092 27.118477335699858 27.007331154330593 21.84665114113546 40-41 24.971065798412525 27.387177994773502 26.224592802774303 21.417163404039663 42-43 26.067036198771138 27.479144469320072 25.32488165007058 21.128937681838213 44-45 27.012678040273503 27.112581029660426 24.997649673710562 20.877091256355506 46-47 27.841332799460528 27.09036519108871 24.52665310906922 20.541648900381542 48-49 29.091225395782967 26.46054527307557 24.146785944935463 20.301443386206 50-51 30.086756011855513 25.921890212346128 24.16459775943797 19.826756016360388 52-53 31.318581513936692 26.081081185888383 23.44135001145618 19.15898728871874 54-55 32.4617091451271 26.43839611290577 22.83244376430549 18.26745097766164 56-57 33.262126033613704 27.153867198906855 22.42029937043499 17.163707397044448 58-59 34.27901016699593 27.422718677623077 22.02633760430481 16.27193355107618 60-61 34.96535537184618 28.149937473208453 21.538436845833107 15.346270309112258 62-63 35.23383582114685 28.774914488877855 21.434179488057104 14.55707020191819 64-65 35.443533549008585 28.914860720254932 21.629874129209632 14.01173160152685 66-67 35.66604456853101 28.797114462462854 21.86115869651674 13.675682272489404 68-69 35.6669681489133 28.291790035107805 22.46970983062469 13.571531985354204 70-71 35.98440979584451 27.79661126218941 22.723194393102872 13.49578454886321 72-73 36.19931312851363 27.038917444563292 23.04886930925098 13.712900117672099 74-75 36.40657131640682 25.900475468138666 23.356174111106313 14.336779104348201 76-77 37.04455174862817 24.514530637377412 23.427547357223048 15.01337025677136 78-79 37.164323443478985 23.40634199024682 23.813157194418878 15.616177371855319 80-81 37.082773586765896 22.54011782895754 23.97145384998705 16.405654734289513 82-83 36.501813907526326 21.89717431327706 24.352730818893907 17.24828096030271 84-85 35.43587162122902 21.703154151899646 24.920499881809892 17.94047434506144 86-87 34.40773393772602 22.10385380989438 25.228780674165723 18.25963157821387 88-89 32.963991700158005 23.422957833261325 25.605580359579434 18.007470107001236 90-91 31.355571967221984 25.362906648975546 25.470183527816598 17.81133785598587 92-93 29.621605629040126 27.68493332556965 25.36132772537941 17.33213332001081 94-95 28.005243433460446 30.540502560234756 24.67457868086401 16.779675325440785 96-97 26.451797535219978 33.581055712245686 23.79003187609951 16.17711487643482 98-99 25.003647204942286 36.62712604187392 22.84169114540146 15.527535607782337 100-101 23.684319128332255 39.93710570699638 21.527142874838756 14.851432289832612 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31696.0 1 18927.0 2 5642.5 3 5048.0 4 4634.5 5 4312.0 6 4300.0 7 4362.0 8 4630.0 9 5075.0 10 5518.5 11 5973.5 12 6459.5 13 6906.5 14 7746.5 15 8904.0 16 10122.0 17 11904.5 18 14804.0 19 17627.5 20 20275.5 21 23843.0 22 28043.5 23 33343.5 24 40566.0 25 49793.5 26 60440.0 27 73567.5 28 90498.5 29 113216.5 30 144948.0 31 184667.5 32 238828.0 33 314136.5 34 407785.0 35 540933.0 36 707412.5 37 917580.0 38 1211102.5 39 1526719.0 40 1937280.0 41 2580484.5 42 3414610.5 43 4474084.0 44 5711708.5 45 6866851.5 46 7882149.0 47 8664376.5 48 9017338.0 49 8852372.5 50 8362814.5 51 7714594.5 52 6916466.0 53 6045370.0 54 5063318.0 55 4074115.5 56 3170314.5 57 2377973.0 58 1745276.0 59 1243839.5 60 864770.5 61 594784.0 62 396567.0 63 258385.0 64 168098.5 65 109318.5 66 71594.5 67 47224.0 68 30612.0 69 19701.5 70 12679.5 71 8266.0 72 5733.5 73 4067.5 74 2956.0 75 2147.5 76 1449.5 77 943.0 78 677.0 79 483.0 80 354.5 81 249.5 82 167.0 83 113.0 84 76.0 85 61.0 86 45.0 87 30.5 88 23.5 89 13.0 90 9.0 91 10.5 92 12.5 93 9.5 94 7.0 95 13.5 96 11.5 97 6.0 98 9.0 99 11.5 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12230492854324539 2 0.09865976456571553 3 0.08326709755235923 4 0.07617795960891866 5 0.07808258158933058 6 0.07079363997764516 7 0.07403201631491231 8 0.08444343083726395 9 0.09112604183665655 10-11 0.08844599133424677 12-13 0.08366065023223543 14-15 0.07598464307275968 16-17 0.06920602251849618 18-19 0.08624036642505041 20-21 0.12266604556269223 22-23 0.16948497525284528 24-25 0.14814317564127225 26-27 0.0984236329577898 28-29 0.07105917991769352 30-31 0.0609392538637337 32-33 0.056245165086396966 34-35 0.06024426577960066 36-37 0.07653734672647595 38-39 0.06263239533814621 40-41 0.07799608649485229 42-43 0.06576654508656701 44-45 0.08903415797669914 46-47 0.14511109010433582 48-49 0.11440965631926715 50-51 0.15827823833676574 52-53 0.18604619099260325 54-55 0.12366290152655453 56-57 0.08127900780577575 58-59 0.06664836257477316 60-61 0.060393037342103315 62-63 0.06602127313980558 64-65 0.05989915035263228 66-67 0.04672378508622692 68-69 0.04757446434042089 70-71 0.04704511436221376 72-73 0.0759483151330788 74-75 0.1015677296420756 76-77 0.11043520672798979 78-79 0.1744013564229912 80-81 0.1720473924267646 82-83 0.10713974362836698 84-85 0.06309081933888115 86-87 0.04629606684403178 88-89 0.041239563620831005 90-91 0.040351691476011364 92-93 0.04305379822751312 94-95 0.02666687010312888 96-97 0.027873044195628617 98-99 0.03346322215176044 100-101 0.18289257984792484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.15613493E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.516906377918747 #Duplication Level Percentage of deduplicated Percentage of total 1 67.1194706592644 14.442033663108564 2 14.010620634157508 6.029304249634074 3 5.463907095049134 3.526991322654547 4 2.8879430098346996 2.4855839746951247 5 1.705392580820884 1.8347386249560103 6 1.215935946421022 1.5697907952412296 7 0.9178507482536437 1.3824516033353424 8 0.680447518137123 1.1712900434274918 9 0.5947400762011983 1.1517269884935701 >10 4.27837572519398 18.620363647148316 >50 0.5915871521851775 8.919429438128038 >100 0.4626737084282303 19.586648642929845 >500 0.04576182363114077 6.724222963187354 >1k 0.023712015147841322 9.077758862713074 >5k 0.0011464528967272487 1.6370619437463634 >10k+ 4.3485437711139873E-4 1.8406032366010163 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.01671547974076E-5 0.0 0.0 1.7299018895657794E-6 0.0 2 1.3147254360699922E-4 0.0 0.0 6.054656613480228E-6 0.0 3 2.0672327580311062E-4 0.0 0.0 6.054656613480228E-6 0.0 4 2.9754312500531404E-4 0.0 0.0 6.9196075582631175E-6 0.0 5 3.874980232627346E-4 0.0 0.0 6.9196075582631175E-6 0.0 6 4.835075781336353E-4 0.0 0.0 6.9196075582631175E-6 0.0 7 5.72597525446273E-4 0.0 0.0 1.2109313226960455E-5 0.0 8 6.469833066976015E-4 0.0 0.0 1.2109313226960455E-5 0.0 9 7.403980087341536E-4 0.0 0.0 1.2974264171743345E-5 0.0 10-11 0.0012623959039106275 0.0 0.0 1.4704166061309125E-5 0.0 12-13 0.0015093393986461423 0.0 0.0 1.5569117006092013E-5 0.0 14-15 0.002440459090704923 4.3247547239144485E-7 0.0 1.6866543423266348E-5 4.3247547239144485E-7 16-17 0.0034965641942848315 8.649509447828897E-7 4.3247547239144485E-7 2.0326347202397907E-5 8.649509447828897E-7 18-19 0.0047304167170176235 1.2974264171743345E-6 8.649509447828897E-7 3.416556231892414E-5 8.649509447828897E-7 20-21 0.05890661914349392 1.7299018895657794E-6 1.7299018895657794E-6 4.800477743545038E-5 8.649509447828897E-7 22-23 0.08934208051304185 1.7299018895657794E-6 1.7299018895657794E-6 6.184399255197661E-5 1.2974264171743345E-6 24-25 0.1776786555527736 1.7299018895657794E-6 1.7299018895657794E-6 6.746617369306539E-5 1.7299018895657794E-6 26-27 0.5576312792486946 2.594852834348669E-6 1.7299018895657794E-6 7.352083030654562E-5 1.7299018895657794E-6 28-29 1.575809148850818 2.594852834348669E-6 1.7299018895657794E-6 7.784558503046007E-5 1.7299018895657794E-6 30-31 3.2455143449389596 3.4598037791315588E-6 1.7299018895657794E-6 8.563014353350608E-5 1.7299018895657794E-6 32-33 5.758051095299058 4.324754723914448E-6 1.7299018895657794E-6 8.86574718402462E-5 1.7299018895657794E-6 34-35 8.855912691782438 4.324754723914448E-6 1.7299018895657794E-6 1.0163173601198953E-4 1.7299018895657794E-6 36-37 12.74241017871504 4.324754723914448E-6 1.7299018895657794E-6 1.0725391715307832E-4 1.7299018895657794E-6 38-39 17.030417461740388 4.324754723914448E-6 1.7299018895657794E-6 1.1417352471134144E-4 1.7299018895657794E-6 40-41 21.48299117647107 4.324754723914448E-6 1.7299018895657794E-6 1.1503847565612433E-4 1.7299018895657794E-6 42-43 25.984883961597802 4.324754723914448E-6 1.7299018895657794E-6 1.2195808321438745E-4 2.594852834348669E-6 44-45 30.425958585993072 4.324754723914448E-6 1.7299018895657794E-6 1.271477888830848E-4 2.594852834348669E-6 46-47 34.83188030656595 4.324754723914448E-6 1.7299018895657794E-6 1.2887769077265057E-4 2.594852834348669E-6 48-49 39.123137642766316 4.324754723914448E-6 1.7299018895657794E-6 1.3147254360699922E-4 2.594852834348669E-6 50-51 43.20516550780107 5.189705668697338E-6 1.7299018895657794E-6 1.340673964413479E-4 2.594852834348669E-6 52-53 47.0513450363445 5.189705668697338E-6 1.7299018895657794E-6 1.3622977380330512E-4 2.594852834348669E-6 54-55 50.712008156349015 5.189705668697338E-6 1.7299018895657794E-6 1.3666224927569656E-4 2.594852834348669E-6 56-57 54.137251523055355 5.189705668697338E-6 1.7299018895657794E-6 1.3666224927569656E-4 2.594852834348669E-6 58-59 57.28850308155641 6.054656613480228E-6 1.7299018895657794E-6 1.3666224927569656E-4 2.594852834348669E-6 60-61 60.25458421189644 6.487132085871673E-6 1.7299018895657794E-6 1.3752720022047946E-4 2.594852834348669E-6 62-63 63.01430707573207 1.0379411337394676E-5 1.7299018895657794E-6 1.3925710211004524E-4 2.594852834348669E-6 64-65 65.58322911323162 1.8163969840440682E-5 1.7299018895657794E-6 1.40987003999611E-4 2.594852834348669E-6 66-67 67.96583250019097 2.8543381177835357E-5 1.7299018895657794E-6 1.4401433230635114E-4 2.594852834348669E-6 68-69 70.17351556016044 4.022021893240437E-5 1.7299018895657794E-6 1.4444680777874258E-4 2.594852834348669E-6 70-71 72.16644816708376 4.886972838023327E-5 1.7299018895657794E-6 1.4444680777874258E-4 2.594852834348669E-6 72-73 73.99305762693287 6.876360011023972E-5 1.7299018895657794E-6 1.4444680777874258E-4 2.594852834348669E-6 74-75 75.69439797135098 8.433271711633174E-5 1.7299018895657794E-6 1.474741360854827E-4 2.594852834348669E-6 76-77 77.25083308398959 9.903688317764088E-5 1.7299018895657794E-6 1.4963651344743992E-4 2.594852834348669E-6 78-79 78.67053848117884 1.1374104923894999E-4 1.7299018895657794E-6 1.505014643922228E-4 2.594852834348669E-6 80-81 79.9669023061175 1.3709472474808803E-4 1.7299018895657794E-6 1.5093393986461426E-4 2.594852834348669E-6 82-83 81.15774946787569 1.5871849836766026E-4 2.594852834348669E-6 1.5179889080939714E-4 2.594852834348669E-6 84-85 82.25237170197772 1.7644999273570946E-4 2.594852834348669E-6 1.5352879269896292E-4 2.594852834348669E-6 86-87 83.24335810872871 1.9331653615897585E-4 2.594852834348669E-6 1.5396126817135436E-4 2.594852834348669E-6 88-89 84.14205468214683 2.11912981471808E-4 2.594852834348669E-6 1.5396126817135436E-4 2.594852834348669E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCATT 22450 0.0 66.302475 1 ACGCGTC 28020 0.0 48.619045 2 CGCGTGT 127170 0.0 47.80116 1 CACGCGT 29085 0.0 46.58861 1 GCGTGTT 139985 0.0 43.245277 2 CGCGCGT 5140 0.0 41.765953 1 CGCGTCT 50840 0.0 35.021717 3 CGTGTTT 178180 0.0 34.185783 3 CCCGCTC 30090 0.0 32.807743 2 CCCCGCT 30210 0.0 32.355022 1 CGGGCGT 9570 0.0 30.96847 1 CCAACGA 10280 0.0 30.761633 8 CCGTACT 9385 0.0 30.357153 2 GGCATTC 49110 0.0 30.340801 2 GCGTCTC 59965 0.0 29.89022 4 CAACGAA 11325 0.0 28.510115 9 CCCGTAC 9270 0.0 27.871878 1 TGAACCG 21805 0.0 27.240967 12-13 CGGGTGT 16340 0.0 26.74132 1 CCGGAAT 10905 0.0 26.654642 1 >>END_MODULE