FastQCFastQC Report
Fri 27 May 2016
SRR1233079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115613493
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTTAAGAGGTACAACATGGAGAAGACATGATCACAGAATGGAAA1915280.16566232455237728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA1294800.045.5730366-67
ATGCGCG1426400.044.06365664-65
AATGCGC1465350.043.4832964-65
ATAGTCG193050.042.86655866-67
GCGCGAG1337800.042.6960366-67
ATGGCCG2361150.041.0238566-67
TGACCGA1252950.039.6454466-67
ATGACCG1279100.039.3483366-67
ATGCACG679650.039.30376464-65
CGATAGT220200.038.79160764-65
CCGGATA626250.038.3014166-67
ATGTCCG647100.037.74019266-67
ATCGTCG163350.036.73540566-67
GGCCGAG2560250.036.10913568-69
ACCGGAT1347100.036.0486764-65
ATGATCG225300.035.8856766-67
CGATCAT248600.035.82402864-65
CGGATCT678150.035.6894266-67
TGCACGA698300.035.68177466-67
ATACTCG249700.035.56165766-67
Produced by FastQC (version 0.11.5)