##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115613493 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.206081940626085 39.0 37.0 39.0 34.0 39.0 2 36.874072864488234 39.0 37.0 39.0 33.0 39.0 3 36.82699653404642 39.0 37.0 39.0 33.0 39.0 4 36.735431737193515 38.0 37.0 39.0 33.0 39.0 5 36.72019912070298 38.0 36.0 39.0 33.0 39.0 6 36.82276970041897 39.0 37.0 39.0 33.0 39.0 7 36.8368809166591 39.0 36.0 39.0 33.0 39.0 8 36.7510153161794 38.0 36.0 39.0 33.0 39.0 9 36.734209708550196 38.0 36.0 39.0 33.0 39.0 10-11 36.69329914199548 38.0 36.0 39.0 33.0 39.0 12-13 36.616710594497825 38.0 36.0 39.0 32.5 39.0 14-15 36.53790270396899 38.0 36.0 39.0 32.0 39.0 16-17 36.422226703244746 38.0 36.0 39.0 31.5 39.0 18-19 36.238202637818404 38.0 36.0 39.0 31.0 39.0 20-21 36.17178599127699 38.0 36.0 39.0 31.0 39.0 22-23 35.991916276588924 38.0 36.0 39.0 31.0 39.0 24-25 35.891556524462075 38.0 35.0 39.0 30.0 39.0 26-27 35.732016997358606 38.0 35.5 39.0 30.0 39.0 28-29 35.55251334288464 38.0 35.0 39.0 30.0 39.0 30-31 35.52420028949389 37.5 35.5 39.0 29.5 39.0 32-33 35.52155421426459 38.0 35.0 39.0 30.0 39.0 34-35 35.37391240311371 38.0 35.0 39.0 29.5 39.0 36-37 35.20971441456232 37.5 35.0 39.0 29.0 39.0 38-39 34.973397832552294 37.0 35.0 39.0 28.5 39.0 40-41 34.77914437720517 37.0 35.0 39.0 28.0 39.0 42-43 34.46998733962653 37.0 34.0 39.0 27.0 39.0 44-45 34.1637564959654 37.0 34.0 39.0 26.5 39.0 46-47 33.769426406829524 37.0 33.5 39.0 25.0 39.0 48-49 33.421042909757944 37.0 33.0 39.0 24.0 39.0 50-51 33.185425208111305 36.5 33.0 39.0 23.5 39.0 52-53 32.91909866437476 36.0 33.0 39.0 22.5 39.0 54-55 32.571105056915805 36.0 32.0 38.5 21.5 39.0 56-57 32.11189708194354 36.0 32.0 38.0 19.0 39.0 58-59 31.72714741868408 36.0 31.0 37.0 17.5 39.0 60-61 31.256156761910134 36.0 31.0 37.0 8.0 39.0 62-63 30.64631657223608 35.0 30.0 37.0 2.0 39.0 64-65 29.900625037771327 35.0 29.5 37.0 2.0 39.0 66-67 27.191947310163876 33.0 24.5 36.0 2.0 37.5 68-69 23.562078005030088 30.0 11.0 35.5 2.0 37.0 70-71 19.96102429411072 26.0 2.0 33.5 2.0 36.0 72-73 18.387675290634114 22.0 2.0 33.0 2.0 36.0 74-75 17.685229997332577 20.5 2.0 32.5 2.0 36.0 76-77 18.40807627877829 22.0 2.0 35.0 2.0 37.0 78-79 17.879304792737297 20.5 2.0 33.5 2.0 36.5 80-81 17.103303249388027 11.5 2.0 33.5 2.0 37.0 82-83 16.30722438686287 2.0 2.0 33.0 2.0 36.0 84-85 15.572438780999377 2.0 2.0 32.0 2.0 36.0 86-87 14.539820412657196 2.0 2.0 31.5 2.0 36.0 88-89 13.876862231815796 2.0 2.0 30.5 2.0 35.0 90-91 12.896150356775397 2.0 2.0 29.5 2.0 35.0 92-93 11.970561550285485 2.0 2.0 28.0 2.0 34.0 94-95 11.237345843361034 2.0 2.0 27.0 2.0 34.0 96-97 9.789076249949476 2.0 2.0 21.0 2.0 33.5 98-99 9.038789542497431 2.0 2.0 12.5 2.0 32.5 100-101 8.499513763501636 2.0 2.0 2.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.41171986632898694 1 2 0.4995703247654433 1 3 0.4872418894855812 1 4 0.5036340292688664 1 5 0.549293420087615 1 6 0.5256476008328335 1 7 0.5219297278291606 1 8 0.5330871523715786 1 9 0.5363793736184377 1 10-11 0.5716059900660895 1 12-13 0.5976547080917527 1 14-15 0.6025837418947617 1 16-17 0.6454786254249072 1 18-19 0.7456087482308007 1 20-21 0.7129051930225074 1 22-23 0.7329895554203318 1 24-25 0.7317171266532725 1 26-27 0.700730245832176 1 28-29 0.7522479743953951 1 30-31 0.7346462922853263 1 32-33 0.7113572445136 1 34-35 0.7347463681699651 1 36-37 0.7170200650079792 1 38-39 0.755606319880691 1 40-41 0.7632267458460333 1 42-43 0.8204752592733797 1 44-45 0.9186370676254683 1 46-47 0.9454193450424313 1 48-49 0.8712071665809162 1 50-51 0.8332466508058403 1 52-53 0.8280648952431306 1 54-55 0.8320875762179725 1 56-57 0.8446674249607113 1 58-59 0.8346528979421137 1 60-61 0.7177910776025733 1 62-63 0.6831761606818922 1 64-65 0.7192959872208036 1 66-67 0.2842659351998904 1 68-69 -0.5637311153944609 1 70-71 -0.9471181368082426 1 72-73 -1.0920973089038597 1 74-75 -1.1013316288625852 1 76-77 -0.9749811918846198 1 78-79 -1.075423917456547 1 80-81 -1.232052914051092 1 82-83 -1.289618914622725 1 84-85 -1.3656020637281099 1 86-87 -1.390796460506058 1 88-89 -1.4274985913686749 1 90-91 -1.3137768506428458 1 92-93 -1.3801318488603105 1 94-95 -1.1736788785557692 1 96-97 -1.1531556345846603 1 98-99 -1.0136980579559998 1 100-101 -0.8855195818666877 2 1 0.3497852454720842 2 2 0.4136387311512948 2 3 0.4029223156842008 2 4 0.395609853125805 2 5 0.4182227456003105 2 6 0.43809897199468395 2 7 0.40974227378545436 2 8 0.41348939129265716 2 9 0.4397516409448272 2 10-11 0.46454706235541465 2 12-13 0.49958920078919533 2 14-15 0.49655976639599686 2 16-17 0.5274763832131768 2 18-19 0.6221083356690684 2 20-21 0.5723114993501781 2 22-23 0.6149162517614428 2 24-25 0.5978798029056023 2 26-27 0.5776300474990705 2 28-29 0.5832648500785851 2 30-31 0.5879106502963865 2 32-33 0.5829667704294366 2 34-35 0.5769031909979319 2 36-37 0.5522602478096488 2 38-39 0.6226316603913418 2 40-41 0.6434540964846391 2 42-43 0.714423566066074 2 44-45 0.7405191640276456 2 46-47 0.73393203877378 2 48-49 0.6981809754338784 2 50-51 0.665840157936465 2 52-53 0.6180205676214925 2 54-55 0.6235100791178354 2 56-57 0.6843528536936603 2 58-59 0.6813703794190573 2 60-61 0.5867511185455498 2 62-63 0.5644682891975705 2 64-65 0.5580078077824986 2 66-67 0.22779393457273045 2 68-69 -0.4388766119864016 2 70-71 -0.8383033538487794 2 72-73 -0.9201563494203668 2 74-75 -0.8931353406654097 2 76-77 -0.6961903034786872 2 78-79 -0.7765366548405552 2 80-81 -0.892085863980352 2 82-83 -0.9027697281134053 2 84-85 -0.9852830167694702 2 86-87 -0.8734875194776421 2 88-89 -0.913685617814723 2 90-91 -0.813076326631311 2 92-93 -0.8262394248049993 2 94-95 -0.6986296364333402 2 96-97 -0.7376966456542373 2 98-99 -0.6660593583764847 2 100-101 -0.5406239810692997 3 1 0.2666855050219823 3 2 0.32672599227500143 3 3 0.3361323022096059 3 4 0.31638062593854954 3 5 0.33706929808388253 3 6 0.3183243243579028 3 7 0.33406457729061145 3 8 0.3456582103112069 3 9 0.36644965310188127 3 10-11 0.35037234066850687 3 12-13 0.3777269532704466 3 14-15 0.38742558154265083 3 16-17 0.4224483771236578 3 18-19 0.4860524489561868 3 20-21 0.4283172682619778 3 22-23 0.42955239213721086 3 24-25 0.42595750042089975 3 26-27 0.3920309619885529 3 28-29 0.40064365491559073 3 30-31 0.42539666683270383 3 32-33 0.3930687276581324 3 34-35 0.4053316234203166 3 36-37 0.37297202417226316 3 38-39 0.39796477499380956 3 40-41 0.4448496373348405 3 42-43 0.43434293100150967 3 44-45 0.5185117972121986 3 46-47 0.5361328877090301 3 48-49 0.4821856790010841 3 50-51 0.45608445161813194 3 52-53 0.40110002207077144 3 54-55 0.3557199864964815 3 56-57 0.37254034904513134 3 58-59 0.3918326688587612 3 60-61 0.36859236806648354 3 62-63 0.4269130036467885 3 64-65 0.44664465775845485 3 66-67 0.29324360288668316 3 68-69 -0.05699254685644206 3 70-71 -0.40337974792769415 3 72-73 -0.4685340967274776 3 74-75 -0.40327332985717845 3 76-77 -0.32064089819266073 3 78-79 -0.3138060976236332 3 80-81 -0.41697338937533246 3 82-83 -0.34420073041197696 3 84-85 -0.3765893200938084 3 86-87 -0.22107118675553217 3 88-89 -0.21202011933695175 3 90-91 -0.16750182559212057 3 92-93 -0.16703323793057834 3 94-95 -0.08114867992215125 3 96-97 -0.14676818552927173 3 98-99 -0.15056989236867224 3 100-101 -0.070431366861758 4 1 0.20143145152332664 4 2 0.24291799670523062 4 3 0.23037796809580868 4 4 0.23133791404873705 4 5 0.24981902426224423 4 6 0.2662056213349686 4 7 0.2365498819727847 4 8 0.2524561225193551 4 9 0.21113590857684983 4 10-11 0.24357821922767897 4 12-13 0.22943238241914088 4 14-15 0.23478371733701664 4 16-17 0.25266217823658366 4 18-19 0.2989305548050254 4 20-21 0.2557056564714628 4 22-23 0.2388601287500336 4 24-25 0.2541703762106735 4 26-27 0.2348986174107779 4 28-29 0.20526791200726535 4 30-31 0.2534462750222275 4 32-33 0.24532907508884705 4 34-35 0.2270951970909465 4 36-37 0.285418566057416 4 38-39 0.29122536196193494 4 40-41 0.32220132593423756 4 42-43 0.34767761789852614 4 44-45 0.3986864874624416 4 46-47 0.39437630659829637 4 48-49 0.3431515561422174 4 50-51 0.30334019351249 4 52-53 0.21534473518676123 4 54-55 0.19045588403213998 4 56-57 0.21727671789792424 4 58-59 0.177166506783486 4 60-61 0.11871838153238912 4 62-63 0.23328340597225505 4 64-65 0.18223328670485017 4 66-67 0.3611224588918063 4 68-69 0.26833480173164403 4 70-71 0.01090581659399703 4 72-73 -0.023380459223524497 4 74-75 0.03680117943570238 4 76-77 0.06870823307803064 4 78-79 0.12028483239171806 4 80-81 0.0784416304126303 4 82-83 0.17206233127659587 4 84-85 0.17687614289343578 4 86-87 0.25008247401305184 4 88-89 0.26037182184832375 4 90-91 0.21422357081704568 4 92-93 0.19203973247704553 4 94-95 0.23805035380237882 4 96-97 0.21155718590248895 4 98-99 0.15574756506917176 4 100-101 0.1571925718961964 5 1 0.07914558799861027 5 2 0.05842815037227922 5 3 0.08023874994536584 5 4 0.07405155897471616 5 5 0.08201212470826391 5 6 0.08012169893802934 5 7 0.11391065853936055 5 8 0.09895683107184539 5 9 0.11134137830268998 5 10-11 0.11217243797191401 5 12-13 0.1109317085230046 5 14-15 0.11682672253445503 5 16-17 0.08085177244442576 5 18-19 0.15445685588325375 5 20-21 0.06860081607286617 5 22-23 0.08309133356677023 5 24-25 0.07445531734271071 5 26-27 0.09253106071251693 5 28-29 0.09705830023579409 5 30-31 0.07369946491320434 5 32-33 0.0903149045326046 5 34-35 0.07595989798250713 5 36-37 0.08750419527680009 5 38-39 0.12193029334627425 5 40-41 0.11657426802862147 5 42-43 0.16437893734536146 5 44-45 0.1732221840640591 5 46-47 0.25156907195252387 5 48-49 0.13511761723497528 5 50-51 0.11884333475396858 5 52-53 0.09584222494577688 5 54-55 0.07557601667714664 5 56-57 0.1390860951347932 5 58-59 0.1406495304915829 5 60-61 0.10523923061098373 5 62-63 0.10790393079306781 5 64-65 0.13286570687214194 5 66-67 0.32602806615948765 5 68-69 0.5480235169723144 5 70-71 0.462588509359243 5 72-73 0.4521416584877649 5 74-75 0.5121456391790211 5 76-77 0.4768351176290544 5 78-79 0.5416221727546038 5 80-81 0.5768707025181037 5 82-83 0.6444964822236621 5 84-85 0.6117669105497807 5 86-87 0.7040771513678674 5 88-89 0.6506631731614796 5 90-91 0.5865463535713733 5 92-93 0.6180974077984516 5 94-95 0.5785438954128654 5 96-97 0.5225876037410302 5 98-99 0.42604987697917984 5 100-101 0.38572402102280634 6 1 -0.052310824391909705 6 2 -0.07427053166654218 6 3 -0.07001405270749927 6 4 -0.10126662990316504 6 5 -0.07096734678508909 6 6 -0.05503214955189151 6 7 -0.08487522776292167 6 8 -0.1331053005959717 6 9 -0.08851594460418966 6 10-11 -0.09780852001714635 6 12-13 -0.15908444821668155 6 14-15 -0.1465823055886588 6 16-17 -0.13669298342426117 6 18-19 -0.092063497710825 6 20-21 -0.14164215010848835 6 22-23 -0.20055609701107358 6 24-25 -0.20816790997356804 6 26-27 -0.19017008275582725 6 28-29 -0.2591011531140168 6 30-31 -0.22196714286867092 6 32-33 -0.29159157627073284 6 34-35 -0.2699966980389945 6 36-37 -0.2775194673799817 6 38-39 -0.25291158973428907 6 40-41 -0.3050953557755207 6 42-43 -0.2514174506427125 6 44-45 -0.22304511240704983 6 46-47 -0.1428972459680793 6 48-49 -0.3045328704402408 6 50-51 -0.1948716238221877 6 52-53 -0.367059763005976 6 54-55 -0.35995725728164274 6 56-57 -0.3318483354310118 6 58-59 -0.32352956725526383 6 60-61 -0.3782819341659902 6 62-63 -0.38105060023682213 6 64-65 -0.38154211307398356 6 66-67 0.04789600262520821 6 68-69 0.431208767222774 6 70-71 0.8303331867342081 6 72-73 0.8511749282282821 6 74-75 0.9703126404621933 6 76-77 0.9463439517597898 6 78-79 0.9401162226632351 6 80-81 1.0129098978474076 6 82-83 1.0555095495344347 6 84-85 1.018872931829092 6 86-87 1.0853612589373576 6 88-89 1.0108729613642868 6 90-91 0.9000012180421475 6 92-93 0.9127909484237389 6 94-95 0.8464093410891333 6 96-97 0.8129155026430102 6 98-99 0.6837454074958185 6 100-101 0.5964926502614158 7 1 -0.23203210331971746 7 2 -0.31135137538851865 7 3 -0.31857738328012175 7 4 -0.3456264297666394 7 5 -0.353218287904447 7 6 -0.4367278845486382 7 7 -0.36736642108760975 7 8 -0.4046763517378409 7 9 -0.32611900271495387 7 10-11 -0.39193836891208633 7 12-13 -0.3777698805457277 7 14-15 -0.38916212360565083 7 16-17 -0.4606729054455698 7 18-19 -0.4102592750924572 7 20-21 -0.44550389973413473 7 22-23 -0.4446204569680816 7 24-25 -0.4196417697855921 7 26-27 -0.39411737975329686 7 28-29 -0.3708976795585386 7 30-31 -0.401014240159391 7 32-33 -0.3444890284640536 7 34-35 -0.3364823210155663 7 36-37 -0.4537663167984718 7 38-39 -0.43418405516043634 7 40-41 -0.5436875235144427 7 42-43 -0.5385393360674442 7 44-45 -0.6924137125813559 7 46-47 -0.4265570970408632 7 48-49 -0.533186264721131 7 50-51 -0.47832118777872523 7 52-53 -0.4875402060705767 7 54-55 -0.7301027037721575 7 56-57 -0.5822448816417207 7 58-59 -0.5868351365539368 7 60-61 -0.5157636183027492 7 62-63 -0.5540541174221758 7 64-65 -0.503410182515907 7 66-67 -0.12221684447457193 7 68-69 0.6239216240323131 7 70-71 1.3108206942216825 7 72-73 1.532120057816158 7 74-75 1.6434712797336495 7 76-77 1.6219935887847718 7 78-79 1.6080303522940866 7 80-81 1.7524365024992967 7 82-83 1.544962037312171 7 84-85 1.6174282855361053 7 86-87 1.637406146961787 7 88-89 1.5546054358713661 7 90-91 1.3854369541386689 7 92-93 1.4379712314970696 7 94-95 1.3161596023352828 7 96-97 1.3303046881674359 7 98-99 1.2059184553344018 7 100-101 1.082716079473947 8 1 -0.5901431908412178 8 2 -0.8341748276287149 8 3 -0.7151705952324576 8 4 -0.9504724888944409 8 5 -0.8307394582993339 8 6 -0.7957811937701678 8 7 -0.7596964675289541 8 8 -0.7477788060661723 8 9 -0.8046586871941557 8 10-11 -0.9443439139674155 8 12-13 -0.9072091363009136 8 14-15 -0.8527153140696839 8 16-17 -1.0029570291501173 8 18-19 -1.0631754278221308 8 20-21 -1.0660119460954292 8 22-23 -1.034232930798666 8 24-25 -1.103047943743448 8 26-27 -1.081883181697492 8 28-29 -1.1315460857205082 8 30-31 -1.5165109763894193 8 32-33 -1.3630767424191177 8 34-35 -1.4176653798788266 8 36-37 -1.1666485120608527 8 38-39 -1.57190542791367 8 40-41 -1.3158963848953604 8 42-43 -1.4136522022153883 8 44-45 -1.4474971354626547 8 46-47 -2.0220361858243017 8 48-49 -1.5570283381764867 8 50-51 -1.453564131085102 8 52-53 -1.4107265949293328 8 54-55 -1.460100071998795 8 56-57 -2.4122079096724605 8 58-59 -1.7464442110474359 8 60-61 -1.467041466327899 8 62-63 -1.3075676616169218 8 64-65 -1.0758580658938968 8 66-67 -0.030410039219205487 8 68-69 1.1709798845212127 8 70-71 2.310929087330308 8 72-73 2.258574767745163 8 74-75 2.47978575316861 8 76-77 2.1978096616769243 8 78-79 2.1468765341264415 8 80-81 2.658113439366101 8 82-83 2.4836762847218132 8 84-85 2.7029758645126467 8 86-87 2.8091285549151443 8 88-89 2.752391998383123 8 90-91 2.510967154015285 8 92-93 2.6238012730022326 8 94-95 2.3777781681725827 8 96-97 2.2596140629687564 8 98-99 2.176606218533946 8 100-101 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0.2859454127756891 65 60-61 0.23394363409082786 65 62-63 0.23194577858521015 65 64-65 0.18975650757724694 65 66-67 0.10073289375321082 65 68-69 0.12272431665843797 65 70-71 -0.2337482680657743 65 72-73 -0.2516153450467087 65 74-75 -0.25967778235130723 65 76-77 -0.26899007341955183 65 78-79 -0.24980640018691247 65 80-81 -0.25607798643388335 65 82-83 -0.17265256839172238 65 84-85 -0.18566287364697054 65 86-87 -0.10724962997081455 65 88-89 -0.09662621888811174 65 90-91 -0.08383968541889608 65 92-93 -0.11936338640616384 65 94-95 -0.10082397034699397 65 96-97 -0.16897198346910258 65 98-99 -0.16904579431253275 65 100-101 -0.108713364446416 66 1 -0.11023240544219703 66 2 -0.1453572224944395 66 3 -0.13084191527607913 66 4 -0.08384828442471814 66 5 -0.10429557273706536 66 6 -0.16697403821601142 66 7 -0.1671155159516573 66 8 -0.14125322415177521 66 9 -0.1565583144625151 66 10-11 -0.16775811128716356 66 12-13 -0.19217359482446028 66 14-15 -0.2070630839173404 66 16-17 -0.17316414037313876 66 18-19 -0.11126852569933021 66 20-21 -0.2173508845615686 66 22-23 -0.20749160567520164 66 24-25 -0.19915165039035543 66 26-27 -0.2206112092019481 66 28-29 -0.2031596724918998 66 30-31 -0.2397788321304617 66 32-33 -0.2041601308989911 66 34-35 -0.18813389531778313 66 36-37 -0.16132185072442695 66 38-39 -0.14776666661096272 66 40-41 -0.12753972113370793 66 42-43 -0.15105046173557923 66 44-45 -0.046499775930513465 66 46-47 -0.13994155704970268 66 48-49 -0.12314407850364262 66 50-51 -0.14836596562663829 66 52-53 -0.10705327077679527 66 54-55 -0.09685959987923809 66 56-57 -0.069205741161106 66 58-59 -0.13075466419516957 66 60-61 -0.14213992803739472 66 62-63 -0.13591117804128672 66 64-65 -0.12560156962400626 66 66-67 -0.045316509067099275 66 68-69 0.16152646650589375 66 70-71 0.10052821787886046 66 72-73 0.06839419923557699 66 74-75 0.1500824921244721 66 76-77 0.06242079806490608 66 78-79 0.08511553896602209 66 80-81 0.14316913823714117 66 82-83 0.18448038476701 66 84-85 0.24742691079566725 66 86-87 0.330793762019038 66 88-89 0.3060314099229 66 90-91 0.27810066334884986 66 92-93 0.2423519876861029 66 94-95 0.19213142379195602 66 96-97 0.13205839848245304 66 98-99 0.09666724312172015 66 100-101 0.09707696258093002 67 1 -0.3116204689659412 67 2 -0.3789140242459581 67 3 -0.3715689601662575 67 4 -0.2980563217182848 67 5 -0.36311867805009257 67 6 -0.385156821570817 67 7 -0.44150713242538586 67 8 -0.40538044499037795 67 9 -0.3831624041076296 67 10-11 -0.3967135451841912 67 12-13 -0.5581486717778503 67 14-15 -0.5103817289704509 67 16-17 -0.5535559024442023 67 18-19 -0.4614128145681917 67 20-21 -0.4787145435074507 67 22-23 -0.5482011643078408 67 24-25 -0.5236796346089889 67 26-27 -0.5175503217337933 67 28-29 -0.5394289608267968 67 30-31 -0.512886825023493 67 32-33 -0.48472458423580633 67 34-35 -0.5271964267677589 67 36-37 -0.530790283455282 67 38-39 -0.587865361803992 67 40-41 -0.6255973193822442 67 42-43 -0.76150422272773 67 44-45 -0.741418751121202 67 46-47 -0.8816697987364179 67 48-49 -0.7675052920516094 67 50-51 -0.7860685470231417 67 52-53 -0.66882733419218 67 54-55 -0.6707825761852817 67 56-57 -0.5519173746612225 67 58-59 -0.6835857603545143 67 60-61 -0.5680066476631005 67 62-63 -0.6293609368151678 67 64-65 -0.7240080943521399 67 66-67 -0.510587023703664 67 68-69 -0.04085850592128537 67 70-71 0.3067704459320737 67 72-73 0.3295116843276631 67 74-75 0.33278076767958353 67 76-77 0.2297091075510096 67 78-79 0.3210736871609967 67 80-81 0.43064448172127 67 82-83 0.4991850092690804 67 84-85 0.5002770252740465 67 86-87 0.6311821998101905 67 88-89 0.5809258281483896 67 90-91 0.5359636567141877 67 92-93 0.5596086238388267 67 94-95 0.5500242742776305 67 96-97 0.5675608171181068 67 98-99 0.5151159758925221 67 100-101 0.4518819688616773 68 1 -0.9801604812560569 68 2 -1.1549284963669493 68 3 -1.2647955151326826 68 4 -1.2754205474404259 68 5 -1.2480732856521115 68 6 -1.1595529862743774 68 7 -1.125362428952748 68 8 -1.179768561801339 68 9 -1.2078182124617243 68 10-11 -1.263061415101042 68 12-13 -1.2954831192461569 68 14-15 -1.3191701473639483 68 16-17 -1.2376245095813871 68 18-19 -1.1905346695309262 68 20-21 -1.2617808609566197 68 22-23 -1.4080597966159871 68 24-25 -1.2813361789407836 68 26-27 -1.5172768789313693 68 28-29 -1.4691671047167034 68 30-31 -1.5355612613339744 68 32-33 -1.5222204010811282 68 34-35 -1.3839234384731327 68 36-37 -1.4623033457257648 68 38-39 -1.6218451164193723 68 40-41 -1.4770981090325606 68 42-43 -1.6191795046078994 68 44-45 -1.7963545071933282 68 46-47 -1.6370400535143546 68 48-49 -1.4157664850513 68 50-51 -1.430481105493719 68 52-53 -1.4832691533625066 68 54-55 -1.1722956681079104 68 56-57 -1.1888587780025475 68 58-59 -1.2273019814794566 68 60-61 -0.8972749717995292 68 62-63 -0.6414052928327365 68 64-65 -0.6101813685778019 68 66-67 -0.18244177063764866 68 68-69 0.9485620493166849 68 70-71 1.6531628358835917 68 72-73 1.9253745872754635 68 74-75 1.7054974536865615 68 76-77 1.8159971445211731 68 78-79 1.997648290138116 68 80-81 2.2541817418544667 68 82-83 2.304588055982748 68 84-85 2.360817669038605 68 86-87 2.5597987360969974 68 88-89 2.587728228175136 68 90-91 2.546000890696437 68 92-93 2.5267119493880763 68 94-95 2.2466640152610644 68 96-97 1.9060134385374816 68 98-99 1.5914273673426091 68 100-101 1.4668472852694379 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 231914.0 3 48841.0 4 85609.0 5 115678.0 6 183430.0 7 245353.0 8 325284.0 9 382876.0 10 476411.0 11 575992.0 12 737091.0 13 760095.0 14 917370.0 15 1024494.0 16 1208422.0 17 1426875.0 18 1690407.0 19 2048416.0 20 2454106.0 21 3044967.0 22 3812977.0 23 4982801.0 24 6428036.0 25 8592621.0 26 1.1443253E7 27 5680079.0 28 4558901.0 29 4661176.0 30 4963967.0 31 5232853.0 32 5407032.0 33 5774373.0 34 6113715.0 35 6848049.0 36 6952535.0 37 5129940.0 38 1047552.0 39 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.685655893850765 16.507486675310002 11.181025433113959 34.62583199772527 2 23.089944501973562 25.912113338029062 32.48699289197652 18.51094926802086 3 28.01680552061172 27.895702428041186 20.8131961981001 23.274295853246993 4 28.85142307156048 33.380885328349294 20.08672100358502 17.680970596505215 5 26.29338380792231 34.49848402375636 23.238070417794972 15.970061750526352 6 22.159152305864506 38.16319692027643 21.9069187711507 17.77073200270837 7 19.483431916406435 20.885347014689255 39.520821670190536 20.110399398713778 8 23.683688892610487 27.103285427073807 26.7969794840469 22.416046196268805 9 22.45876612343163 26.47716646706626 28.528284324045117 22.53578308545699 10-11 25.406657767878354 35.62048635620758 19.70690133892936 19.26595453698471 12-13 23.42817892371784 29.136260505510375 26.146729257630852 21.288831313140932 14-15 23.844911856438763 30.563880636319844 25.125180674196912 20.466026833044477 16-17 23.966503736577284 31.00403700718582 24.085146030857945 20.94431322537895 18-19 23.856522525446056 31.35235218608956 24.087973018858623 20.703152269605763 20-21 23.945615793723807 31.27246600704917 23.742685197047432 21.039233002179586 22-23 23.884624343512915 31.391803221545032 23.89110196242704 20.83247047251501 24-25 24.069109114455358 31.171548139773847 23.80874564119962 20.950597104571177 26-27 23.726000342324397 31.524867058021776 23.637328748548907 21.111803851104916 28-29 23.949406917070604 31.702479598189505 23.358548461409125 20.989565023330766 30-31 23.981509906599 31.706467596150755 23.17549109931618 21.13653139793406 32-33 24.369899399722588 31.849828411818397 22.759285281797688 21.02098690666132 34-35 25.158515073210765 31.996583058337336 22.2981595450159 20.546742323436 36-37 25.580877494203186 32.4227040030496 21.72975052824816 20.26666797449905 38-39 26.488501046342343 32.750221891611794 21.002288167708866 19.758988894336994 40-41 27.395272274751886 32.80961066201149 20.267056028066712 19.528061035169916 42-43 28.64045435009212 32.593551458786756 19.71639352673027 19.04960066439085 44-45 29.402021274260342 32.018029263477914 19.53524744208252 19.044702020179216 46-47 30.251286267708238 31.853104930395325 19.061276629696273 18.834332172200163 48-49 30.63757273229857 31.509596082339435 18.69011996041456 19.162711224947437 50-51 31.361306911919417 31.017584473219806 18.480984186482484 19.140124428378297 52-53 31.91795737160707 30.904889845573514 18.272280375688055 18.904872407131364 54-55 32.30157757968489 30.763281115138817 17.83973955513389 19.095401750042402 56-57 32.4317929471907 30.84948416823852 17.404478380910522 19.314244503660262 58-59 33.11005817332286 30.592262390273444 17.06026886619246 19.237410570211235 60-61 33.33786138700309 30.66796285284473 16.2874060226493 19.70676973750288 62-63 33.25588844268215 30.565693839472313 16.005797640998644 20.172620076846897 64-65 33.31941988605862 30.302554421383938 15.493466749037873 20.88455894351956 66-67 33.116451799421945 30.198540582067086 15.103605716048884 21.581401902462083 68-69 35.15262616373141 30.796248233859234 14.262925711599289 19.788199890810063 70-71 33.971229091934504 27.593165866010665 16.542184168120368 21.893420873934463 72-73 31.08352736016844 28.513596620160925 15.259556309405815 25.143319710264823 74-75 35.766572582877565 29.054009121882146 14.580669840775693 20.598748454464598 76-77 27.997862731879124 29.395131163498654 17.984103183390165 24.622902921232058 78-79 32.113519583943294 25.56198636747115 18.53091302257108 23.793581026014476 80-81 28.52442601071912 26.725472552758532 20.244037560480287 24.506063876042063 82-83 29.24826565089039 25.75327695933116 21.74847757549711 23.249979814281343 84-85 28.480955023095543 22.1002849276401 21.920631068385735 27.498128980878622 86-87 26.68441655001132 22.714855856593434 24.340976915814316 26.25975067758093 88-89 27.089740456512644 22.705814559412662 26.46260975346813 23.741835230606565 90-91 25.70158752646786 19.53470388823043 27.490632997193593 27.27307558810812 92-93 24.934886015723873 21.60926653810789 27.341058547852352 26.114788898315886 94-95 22.76447650386362 24.736502253903573 28.29755072451277 24.201470517720036 96-97 20.98840888211635 26.78857563796642 29.507366449894334 22.715649030022895 98-99 20.1809793266013 28.93719190935004 26.80033277243405 24.081495991614613 100-101 19.041879907827074 32.86913374883124 29.065254858940698 19.023731484400987 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 336.0 1 230.5 2 103.0 3 86.5 4 93.5 5 144.0 6 235.0 7 329.5 8 457.5 9 633.5 10 894.0 11 1172.5 12 1453.5 13 1935.0 14 2411.0 15 2946.5 16 3759.5 17 4837.5 18 6181.5 19 7965.5 20 10375.5 21 13592.5 22 17745.5 23 23585.5 24 31393.0 25 41599.0 26 54831.0 27 72189.0 28 93056.5 29 120283.0 30 159217.5 31 204003.5 32 261855.0 33 334215.0 34 418370.5 35 526684.5 36 646389.0 37 804933.0 38 1013469.0 39 1242997.0 40 1546065.0 41 1931567.0 42 2411713.5 43 3054453.0 44 3902859.5 45 4894680.0 46 5995657.0 47 7122049.0 48 8055882.5 49 8669151.5 50 8875810.0 51 8691019.0 52 8213879.5 53 7490564.5 54 6556293.5 55 5495416.5 56 4418684.5 57 3434324.5 58 2599719.0 59 1910470.5 60 1362863.5 61 951114.0 62 646699.0 63 430815.0 64 283313.5 65 186387.5 66 124023.5 67 81561.5 68 52207.5 69 33296.0 70 21298.5 71 13510.5 72 8945.5 73 6123.0 74 4226.0 75 3008.5 76 2128.5 77 1495.0 78 1042.0 79 744.5 80 554.5 81 345.0 82 228.5 83 148.5 84 90.0 85 63.5 86 44.5 87 26.5 88 19.5 89 16.0 90 12.0 91 9.0 92 5.0 93 4.5 94 5.0 95 4.5 96 2.5 97 5.0 98 9.5 99 9.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023300913501506263 2 0.0029719714462740085 3 0.0132242349947856 4 0.17193235395110845 5 0.001317320288904341 6 0.0 7 8.649509447828897E-7 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.3247547239144485E-7 18-19 0.0 20-21 6.443884538632527E-5 22-23 1.9893871730006462E-5 24-25 1.0163173601198954E-4 26-27 8.065667560100446E-4 28-29 0.0016248103497746582 30-31 0.0037556170022473067 32-33 0.0021878934148283195 34-35 6.482807331147758E-4 36-37 0.0020304723428778335 38-39 0.002102263271294813 40-41 8.104590352615677E-4 42-43 0.0017536880405473086 44-45 0.0014453330287322087 46-47 0.003969259885608681 48-49 0.006363444100767719 50-51 0.006043844726670441 52-53 0.005912804658535834 54-55 0.004851077373814837 56-57 0.004588997237545621 58-59 0.003955853145964546 60-61 0.0039670975082467235 62-63 0.0038083790098790635 64-65 0.0030403025709118572 66-67 0.0017216848555903419 68-69 0.0564808642188503 70-71 0.22575133163738942 72-73 0.2546355034874692 74-75 0.19188633977177733 76-77 0.18415151594805634 78-79 0.160095932747227 80-81 0.13370930675020778 82-83 0.12282606148747707 84-85 0.11559809891739886 86-87 0.11009787585952446 88-89 0.09560908258346627 90-91 0.09470996607636446 92-93 0.09371829981817088 94-95 0.09398773203747074 96-97 0.10802458844487987 98-99 0.12212069399200663 100-101 0.1335726445009321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.15613493E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.450476119740774 #Duplication Level Percentage of deduplicated Percentage of total 1 65.59147223845342 13.413768366711315 2 14.56610249946389 5.957674626459654 3 5.7526391778144825 3.5293263039414082 4 2.9299768526260634 2.396776866240902 5 1.8674166937838736 1.909478025091619 6 1.2696044986728288 1.5578409888974485 7 0.9588528231461789 1.372629773346787 8 0.7144626123459044 1.1688880473782022 9 0.6230467022236036 1.1467441534776341 >10 4.517942678413123 18.694210350761402 >50 0.623418682545754 8.94714012812387 >100 0.5092817447876763 20.445584139936145 >500 0.04950076722348502 6.930018235649212 >1k 0.024639963641153312 9.080271792727059 >5k 0.0012141924164044884 1.6878788555365898 >10k+ 4.278724422856002E-4 1.7617693457209946 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACTTAAGAGGTACAACATGGAGAAGACATGATCACAGAATGGAAA 191528 0.16566232455237728 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.930220385262471E-5 0.0 0.0 0.0 0.0 2 1.2887769077265057E-4 0.0 0.0 0.0 0.0 3 2.0326347202397906E-4 0.0 0.0 0.0 0.0 4 2.9494827217096535E-4 0.0 0.0 8.649509447828897E-7 0.0 5 3.849031704283859E-4 0.0 0.0 8.649509447828897E-7 0.0 6 4.8177767624406955E-4 0.0 0.0 8.649509447828897E-7 0.0 7 5.700026726119243E-4 0.0 0.0 2.594852834348669E-6 0.0 8 6.469833066976015E-4 0.0 0.0 3.4598037791315588E-6 0.0 9 7.395330577893706E-4 0.0 0.0 3.4598037791315588E-6 0.0 10-11 0.0012623959039106275 0.0 0.0 3.4598037791315588E-6 0.0 12-13 0.0015041496929774452 0.0 0.0 3.4598037791315588E-6 0.0 14-15 0.0024348369095638345 0.0 0.0 3.892279251523003E-6 0.0 16-17 0.0034844548810578713 8.649509447828897E-7 4.3247547239144485E-7 4.757230196305893E-6 0.0 18-19 0.00472046978115262 1.2974264171743345E-6 8.649509447828897E-7 1.2974264171743344E-5 0.0 20-21 0.06209136852218451 1.7299018895657794E-6 8.649509447828897E-7 2.421862645392091E-5 0.0 22-23 0.0928481591677193 1.7299018895657794E-6 8.649509447828897E-7 2.767843023305247E-5 0.0 24-25 0.18171408418565815 1.7299018895657794E-6 8.649509447828897E-7 3.027328306740114E-5 0.0 26-27 0.5630307355215018 2.162377361957224E-6 8.649509447828897E-7 3.243566042935836E-5 0.0 28-29 1.6396260945078445 2.594852834348669E-6 8.649509447828897E-7 3.416556231892414E-5 0.0 30-31 3.3313075317255576 2.594852834348669E-6 8.649509447828897E-7 4.281507176675304E-5 0.0 32-33 5.8662205630271895 2.594852834348669E-6 1.2974264171743345E-6 4.930220385262471E-5 0.0 34-35 8.971848121568302 2.594852834348669E-6 1.7299018895657794E-6 5.795171330045361E-5 0.0 36-37 12.862418230024414 2.594852834348669E-6 1.7299018895657794E-6 6.66012227482825E-5 0.0 38-39 17.151130448069758 2.594852834348669E-6 2.162377361957224E-6 7.092597747219695E-5 8.649509447828897E-7 40-41 21.60480092059843 2.594852834348669E-6 2.594852834348669E-6 7.179092841697984E-5 8.649509447828897E-7 42-43 26.102835159560485 2.594852834348669E-6 2.594852834348669E-6 7.438578125132851E-5 1.7299018895657794E-6 44-45 30.531105915120133 2.594852834348669E-6 2.594852834348669E-6 7.52507321961114E-5 1.7299018895657794E-6 46-47 34.92222962245419 2.594852834348669E-6 2.594852834348669E-6 7.91430114476344E-5 1.7299018895657794E-6 48-49 39.19413973592165 2.594852834348669E-6 2.594852834348669E-6 8.087291333720018E-5 1.7299018895657794E-6 50-51 43.25738475871497 3.4598037791315588E-6 2.594852834348669E-6 8.173786428198308E-5 1.7299018895657794E-6 52-53 47.08434637469175 3.4598037791315588E-6 2.594852834348669E-6 8.260281522676596E-5 1.7299018895657794E-6 54-55 50.724709528497684 3.4598037791315588E-6 2.594852834348669E-6 8.303529069915741E-5 1.7299018895657794E-6 56-57 54.12757877663985 3.4598037791315588E-6 2.594852834348669E-6 8.303529069915741E-5 1.7299018895657794E-6 58-59 57.24645997850787 3.4598037791315588E-6 2.594852834348669E-6 8.433271711633174E-5 1.7299018895657794E-6 60-61 60.17780554385637 3.892279251523003E-6 2.594852834348669E-6 8.476519258872319E-5 1.7299018895657794E-6 62-63 62.42784438664093 4.324754723914448E-6 2.594852834348669E-6 8.476519258872319E-5 1.7299018895657794E-6 64-65 64.73280372213995 4.324754723914448E-6 3.027328306740114E-6 8.606261900589751E-5 1.7299018895657794E-6 66-67 66.62610565706203 4.324754723914448E-6 3.4598037791315588E-6 8.822499636785475E-5 1.7299018895657794E-6 68-69 68.64912601507507 4.324754723914448E-6 3.4598037791315588E-6 8.822499636785475E-5 6.9196075582631175E-6 70-71 70.25288302637824 4.324754723914448E-6 3.4598037791315588E-6 8.952242278502907E-5 7.352083030654562E-6 72-73 71.80195135182015 5.189705668697338E-6 3.4598037791315588E-6 9.557707939850932E-5 7.784558503046006E-6 74-75 73.33231338317924 5.189705668697338E-6 3.892279251523003E-6 9.600955487090075E-5 7.784558503046006E-6 76-77 74.70053257538028 5.189705668697338E-6 4.324754723914448E-6 9.773945676046653E-5 9.514460392611786E-6 78-79 75.97214972131323 5.189705668697338E-6 4.324754723914448E-6 1.0206421148438098E-4 1.081188680978612E-5 80-81 77.11313159615375 5.189705668697338E-6 4.324754723914448E-6 1.0336163790155531E-4 1.2109313226960455E-5 82-83 78.169247079145 5.189705668697338E-6 4.324754723914448E-6 1.05956490735904E-4 1.2109313226960455E-5 84-85 79.12919818104622 5.189705668697338E-6 4.324754723914448E-6 1.0768639262546977E-4 1.2109313226960455E-5 86-87 79.99684690782588 5.189705668697338E-6 4.324754723914448E-6 1.1071372093220988E-4 1.427169058891768E-5 88-89 80.77352095918424 5.189705668697338E-6 4.324754723914448E-6 1.1071372093220988E-4 1.5136641533700568E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCGA 129480 0.0 45.57303 66-67 ATGCGCG 142640 0.0 44.063656 64-65 AATGCGC 146535 0.0 43.48329 64-65 ATAGTCG 19305 0.0 42.866558 66-67 GCGCGAG 133780 0.0 42.69603 66-67 ATGGCCG 236115 0.0 41.02385 66-67 TGACCGA 125295 0.0 39.64544 66-67 ATGACCG 127910 0.0 39.34833 66-67 ATGCACG 67965 0.0 39.303764 64-65 CGATAGT 22020 0.0 38.791607 64-65 CCGGATA 62625 0.0 38.30141 66-67 ATGTCCG 64710 0.0 37.740192 66-67 ATCGTCG 16335 0.0 36.735405 66-67 GGCCGAG 256025 0.0 36.109135 68-69 ACCGGAT 134710 0.0 36.04867 64-65 ATGATCG 22530 0.0 35.88567 66-67 CGATCAT 24860 0.0 35.824028 64-65 CGGATCT 67815 0.0 35.68942 66-67 TGCACGA 69830 0.0 35.681774 66-67 ATACTCG 24970 0.0 35.561657 66-67 >>END_MODULE