FastQCFastQC Report
Fri 27 May 2016
SRR1233078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112483704
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1562440.1389036762160677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT1214800.061.0420571
CGGCATT209650.054.7767521
GCGTGTT1468050.052.252422
CGTGTTT2153000.036.0045933
CACGCGT71050.033.0352331
CGGGTGT219450.030.831091
GGCGGAT169700.029.818261
CGGGGCT168450.028.3189541
GCGGATT185800.028.2317561
ACGCGTC82400.027.9482652
CGGGCGT64950.027.87151
TGAACCG182450.023.90720712-13
GGCATTC492500.023.1293962
CGCGTCT163250.023.1225663
CCCCGCT232500.023.0311431
GTGTTTT3573600.022.787964
CGCGCGT67950.022.235771
GAACCGA131500.022.1916514-15
CGGATTT215850.022.1742422
GCGGGCT91950.022.0643271
Produced by FastQC (version 0.11.5)