##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233078_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 112483704 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.16070256719142 39.0 37.0 39.0 33.0 39.0 2 36.86898531541956 39.0 37.0 39.0 33.0 39.0 3 36.81205842937035 39.0 37.0 39.0 33.0 39.0 4 36.74627889209623 39.0 37.0 39.0 33.0 39.0 5 36.746518606819706 39.0 37.0 39.0 33.0 39.0 6 36.810865678818686 39.0 37.0 39.0 33.0 39.0 7 36.812721956595595 39.0 37.0 39.0 33.0 39.0 8 36.74411410740884 39.0 37.0 39.0 33.0 39.0 9 36.72802800839489 39.0 37.0 39.0 33.0 39.0 10-11 36.688797250133234 39.0 36.5 39.0 32.5 39.0 12-13 36.60764417039468 39.0 36.0 39.0 32.0 39.0 14-15 36.522151084213945 39.0 36.0 39.0 32.0 39.0 16-17 36.39572947828958 39.0 36.0 39.0 31.5 39.0 18-19 36.27007735271591 39.0 36.0 39.0 31.0 39.0 20-21 36.084822949109146 39.0 36.0 39.0 31.0 39.0 22-23 35.858424714570205 38.0 36.0 39.0 30.0 39.0 24-25 35.638459274065156 38.0 35.5 39.0 29.5 39.0 26-27 35.30880670501391 38.0 35.5 39.0 28.5 39.0 28-29 35.06659664230118 38.0 35.0 39.0 28.0 39.0 30-31 34.8352745700835 38.0 35.0 39.0 27.5 39.0 32-33 34.660990453337135 38.0 35.0 39.0 27.0 39.0 34-35 34.520204339999324 37.0 34.5 39.0 26.5 39.0 36-37 34.845644098810965 38.0 35.0 39.0 27.5 39.0 38-39 34.65870056608378 38.0 35.0 39.0 27.0 39.0 40-41 34.61775948452053 37.5 35.0 39.0 27.0 39.0 42-43 34.499636569578115 37.5 35.0 39.0 27.0 39.0 44-45 34.32597001339856 37.0 34.0 39.0 26.5 39.0 46-47 34.00094682159471 37.0 34.0 39.0 25.5 39.0 48-49 33.78985159041349 37.0 33.5 39.0 25.0 39.0 50-51 33.80955800939841 37.5 34.0 39.0 24.5 39.0 52-53 33.899012242697836 37.0 34.0 39.0 24.0 39.0 54-55 33.76079668393565 37.0 34.0 39.0 24.0 39.0 56-57 34.07059628388482 38.0 35.0 39.0 25.0 39.0 58-59 33.89211139864313 37.5 34.0 39.0 24.0 39.0 60-61 33.755243497315846 37.0 34.0 39.0 23.5 39.0 62-63 33.69852759738424 37.0 34.0 39.0 23.0 39.0 64-65 33.52042603433472 37.0 34.0 39.0 23.0 39.0 66-67 33.43013167489577 37.0 34.0 39.0 22.0 39.0 68-69 33.19953279632399 37.0 33.0 39.0 21.5 39.0 70-71 32.93231121727642 37.0 33.0 39.0 20.0 39.0 72-73 32.591620053692395 37.0 33.0 39.0 18.5 39.0 74-75 32.27549804903295 36.5 32.0 39.0 17.0 39.0 76-77 31.933552121469965 36.0 32.0 39.0 13.0 39.0 78-79 31.53933130615969 36.0 31.0 38.5 10.0 39.0 80-81 31.07652653401243 36.0 30.5 38.0 2.0 39.0 82-83 30.586806249730184 35.5 29.5 37.0 2.0 39.0 84-85 30.093203105224916 35.0 29.0 37.0 2.0 39.0 86-87 29.39571511620919 34.5 27.5 37.0 2.0 39.0 88-89 28.763334709354876 34.0 27.0 37.0 2.0 39.0 90-91 27.973743556666662 34.0 25.0 37.0 2.0 39.0 92-93 27.026907675444257 33.0 23.0 36.0 2.0 38.5 94-95 26.108950550739333 32.5 20.0 36.0 2.0 37.5 96-97 24.737848399800207 32.0 2.0 36.0 2.0 37.0 98-99 23.72516790076543 31.0 2.0 35.5 2.0 37.0 100-101 22.919552884744974 30.0 2.0 35.5 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.23007728750074108 1 2 0.3754024742887552 1 3 0.2412469488872233 1 4 0.4287221519869888 1 5 0.4012001923434525 1 6 0.3933379021804413 1 7 0.3947285806587004 1 8 0.4459850426284575 1 9 0.4395467472900023 1 10-11 0.47747675178275273 1 12-13 0.38759971812555705 1 14-15 0.48346464223770624 1 16-17 0.3844665950093784 1 18-19 0.2839819591507151 1 20-21 0.22311153537524575 1 22-23 -0.040263060775956205 1 24-25 -0.023962634299330432 1 26-27 -0.46237177554788644 1 28-29 -0.4069765684903217 1 30-31 -0.19519171142037806 1 32-33 -0.3779211867200871 1 34-35 -0.15084286858643026 1 36-37 -0.16651753997562935 1 38-39 -0.10963036762886702 1 40-41 0.06825980360249417 1 42-43 0.08818859744258134 1 44-45 0.21890664154088313 1 46-47 -0.0671605706186611 1 48-49 0.08929354995119354 1 50-51 0.08077397754907878 1 52-53 -0.024303515152823252 1 54-55 -0.013815351923859964 1 56-57 -0.3309548531577917 1 58-59 -0.41186996333428993 1 60-61 -0.2835273244317946 1 62-63 -0.15023880419274604 1 64-65 -0.15270474517998167 1 66-67 0.09552289944913639 1 68-69 0.07562756235697776 1 70-71 0.04666214871895846 1 72-73 0.06148462816628353 1 74-75 -0.018700661933664264 1 76-77 -0.14157820042716907 1 78-79 -0.1653488838648478 1 80-81 -0.27272816450595627 1 82-83 -0.3538037497686197 1 84-85 -0.47965630441297336 1 86-87 -0.6565335438166748 1 88-89 -0.9230298815373779 1 90-91 -1.0383683315610135 1 92-93 -1.1038280534902292 1 94-95 -1.29945044875128 1 96-97 -1.3769584199581786 1 98-99 -1.5749134437689563 1 100-101 -1.5232517481742072 2 1 0.15013718220006922 2 2 0.20144762507018754 2 3 0.15572185344745293 2 4 0.14372227279566374 2 5 0.19111322413412068 2 6 0.19479877334244833 2 7 0.18223364148365562 2 8 0.21156319027021198 2 9 0.18687757033305274 2 10-11 0.1592429449239816 2 12-13 0.16017030865637594 2 14-15 0.17593847971829746 2 16-17 0.23945809924591543 2 18-19 0.21732771201599377 2 20-21 0.22505111061701655 2 22-23 0.2389999010391648 2 24-25 0.28872001741569164 2 26-27 0.3128029294702017 2 28-29 0.3505722592632523 2 30-31 0.378774357231336 2 32-33 0.27244649191331405 2 34-35 0.2508559759424429 2 36-37 0.15649945838016066 2 38-39 0.11193383818180536 2 40-41 0.13754560033781615 2 42-43 0.09407673864436816 2 44-45 0.002720657800750814 2 46-47 0.06529212723906852 2 48-49 0.07379924823598571 2 50-51 0.14206474830484694 2 52-53 0.16701873624534613 2 54-55 0.2036484838327155 2 56-57 0.12235613160220993 2 58-59 0.1454434160323288 2 60-61 0.11472768127923416 2 62-63 0.0800931653579795 2 64-65 0.03668947237610354 2 66-67 -0.22556086327871583 2 68-69 -0.24727716363224062 2 70-71 -0.22055270962164997 2 72-73 -0.23870860802919935 2 74-75 -0.257915346339864 2 76-77 -0.2393454138924369 2 78-79 -0.2745090292631218 2 80-81 -0.39446257578302024 2 82-83 -0.3770218655038171 2 84-85 -0.43314649797737204 2 86-87 -0.4812673552415667 2 88-89 -0.5021457126474438 2 90-91 -0.5138391773402482 2 92-93 -0.6158580966001814 2 94-95 -0.7448403900909142 2 96-97 -0.9001693391219874 2 98-99 -0.9761255650117953 2 100-101 -0.9543048461608308 3 1 0.0684466901467573 3 2 0.1745387016740878 3 3 0.2016531640542496 3 4 0.18452464537827495 3 5 0.1283940428571313 3 6 0.1290597236410278 3 7 0.10080275399035088 3 8 0.16340693724822586 3 9 0.14970219219890168 3 10-11 0.15270870049970853 3 12-13 0.06782422171995961 3 14-15 0.14627962232686542 3 16-17 0.19430463297946687 3 18-19 0.22104084093835752 3 20-21 0.24583236762345706 3 22-23 0.3465343002757635 3 24-25 0.3779054245676363 3 26-27 0.5008456238909886 3 28-29 0.5258733491600083 3 30-31 0.4974556042332239 3 32-33 0.49398222430851035 3 34-35 0.45729554649541626 3 36-37 0.3548278701352814 3 38-39 0.40282953129862165 3 40-41 0.3543011110457499 3 42-43 0.3275484178221433 3 44-45 0.3004781519082087 3 46-47 0.36326088651106403 3 48-49 0.3739685538316735 3 50-51 0.330068560807959 3 52-53 0.3452135500764584 3 54-55 0.3596204565291288 3 56-57 0.3454826259741566 3 58-59 0.35434639853786365 3 60-61 0.3640584789460135 3 62-63 0.33092534115021266 3 64-65 0.29920474653579987 3 66-67 0.2698812865576272 3 68-69 0.23916137445397112 3 70-71 0.22769059407246317 3 72-73 0.18964536677537325 3 74-75 0.1642881314561606 3 76-77 0.13603486784651864 3 78-79 0.1829205031795631 3 80-81 0.24851461983515222 3 82-83 0.2086447845013346 3 84-85 0.17821560758945765 3 86-87 0.1255211071007416 3 88-89 0.22035016687965125 3 90-91 0.257944161595713 3 92-93 0.3924462533395108 3 94-95 0.24990325158042026 3 96-97 0.29221276626184434 3 98-99 0.26698467247991786 3 100-101 0.18517905391859912 4 1 0.1197288968607495 4 2 0.08930072837988234 4 3 0.04589761657010882 4 4 -0.020080727151864153 4 5 0.1265144933604958 4 6 0.088453289050058 4 7 0.10288780988185664 4 8 0.06528483463672075 4 9 0.09012534744594802 4 10-11 0.11205263333848592 4 12-13 0.1426554663215711 4 14-15 0.127016747228069 4 16-17 0.204352929804557 4 18-19 0.1110591432018424 4 20-21 0.05787388171718533 4 22-23 0.03273461357598251 4 24-25 0.12713616187796362 4 26-27 0.25840997091696494 4 28-29 0.21024149210671794 4 30-31 0.128197448886894 4 32-33 0.12282462008252537 4 34-35 0.1674660604538687 4 36-37 0.17725529384073013 4 38-39 0.14893785306681906 4 40-41 0.024009787008800743 4 42-43 0.10550267887791165 4 44-45 0.048250589790981735 4 46-47 0.1961928926964518 4 48-49 0.17336976625778533 4 50-51 0.1958511767404616 4 52-53 0.1924023725575026 4 54-55 0.253848943026064 4 56-57 0.23830799104531053 4 58-59 0.25614539844165307 4 60-61 0.2555034840931114 4 62-63 0.22744652746535365 4 64-65 0.1771426625153012 4 66-67 0.21069119934414715 4 68-69 0.17604954316211519 4 70-71 0.09615126027028253 4 72-73 0.22671872976853535 4 74-75 0.23386012734491857 4 76-77 0.2575442539426973 4 78-79 0.3196210529098842 4 80-81 0.3425167858113234 4 82-83 0.3549195147955011 4 84-85 0.43914989110454883 4 86-87 0.49150003975430323 4 88-89 0.5529642653962021 4 90-91 0.6470413507801958 4 92-93 0.7680966006974721 4 94-95 0.6772972923083458 4 96-97 0.711855823560505 4 98-99 0.7213741376761433 4 100-101 0.7050514424540282 5 1 0.06504235982374951 5 2 -0.05444305594884469 5 3 0.023923300762099586 5 4 -0.023056773989672763 5 5 0.04538845194530694 5 6 0.03548768458695406 5 7 0.02305822285084247 5 8 0.0195087236480731 5 9 0.010918504870709 5 10-11 0.020254434389151754 5 12-13 -0.01630969955381545 5 14-15 -0.026048551955177857 5 16-17 -0.007415224617737692 5 18-19 -0.25388004222943295 5 20-21 -0.25024135613279697 5 22-23 -0.3067991991932928 5 24-25 -0.24279361749211148 5 26-27 -0.02329415119074696 5 28-29 -0.19781943418951187 5 30-31 -0.5145819777654026 5 32-33 -0.4900242929599443 5 34-35 -0.451503347523726 5 36-37 -0.0613482797888949 5 38-39 -0.29968358075331736 5 40-41 -0.12605044255730746 5 42-43 -0.04877394381074396 5 44-45 -0.06971274914338466 5 46-47 0.061142927308630135 5 48-49 0.09427031710493594 5 50-51 0.128188510836452 5 52-53 0.15601178172223484 5 54-55 0.22163605794516883 5 56-57 0.13185943873241257 5 58-59 0.2149521816101796 5 60-61 0.22310793481521785 5 62-63 0.26907114831849555 5 64-65 0.24326853063204368 5 66-67 0.22883116046671859 5 68-69 0.30563730082280927 5 70-71 0.3109294146143995 5 72-73 0.44142134744463135 5 74-75 0.47240753872534924 5 76-77 0.5355200355091512 5 78-79 0.5854924785561018 5 80-81 0.6456426804427657 5 82-83 0.7188617515983502 5 84-85 0.8041605042354618 5 86-87 0.8594590768505661 5 88-89 0.8558564063816512 5 90-91 0.9771434858213901 5 92-93 1.0652769970023819 5 94-95 1.02257282127092 5 96-97 1.10930299777797 5 98-99 1.1591057726486227 5 100-101 1.1202552242015251 6 1 -0.20499400809575263 6 2 -0.2331060380730392 6 3 -0.27010027902788636 6 4 -0.23413430311629213 6 5 -0.22821866178250616 6 6 -0.19475353191295142 6 7 -0.291961435946007 6 8 -0.21417490575218778 6 9 -0.23300276045926438 6 10-11 -0.26204966488567294 6 12-13 -0.3123879737013482 6 14-15 -0.25246192253115396 6 16-17 -0.1611114677437513 6 18-19 -0.13242554750836888 6 20-21 -0.10979337316233995 6 22-23 -0.04570923870090837 6 24-25 0.026134639517316316 6 26-27 0.13463110956553948 6 28-29 0.1568836023360518 6 30-31 0.14944431330994945 6 32-33 0.07927038023120758 6 34-35 0.12004482840645636 6 36-37 1.8924415432763908E-4 6 38-39 -0.0452868413878349 6 40-41 -0.12476128124331609 6 42-43 -0.04002025860928882 6 44-45 -0.0669152623142466 6 46-47 -0.014132441841745447 6 48-49 -0.045091453836093365 6 50-51 -0.06121329844449974 6 52-53 -0.10066633430721339 6 54-55 -0.055646521304687724 6 56-57 0.06785213561660441 6 58-59 0.04646846707679941 6 60-61 0.0652399457422348 6 62-63 0.11320427229032504 6 64-65 0.12202072357167282 6 66-67 -0.02493096452192134 6 68-69 0.03949395086483065 6 70-71 0.016212972539733528 6 72-73 0.09590093069986239 6 74-75 0.09984197448042664 6 76-77 0.18526059334261902 6 78-79 0.24447209623430055 6 80-81 0.301070888334781 6 82-83 0.3876562063676161 6 84-85 0.5260073715157247 6 86-87 0.48402381072765266 6 88-89 0.6088064245872928 6 90-91 0.8328289153775223 6 92-93 1.0223972378430268 6 94-95 0.981290884292676 6 96-97 1.2238953605907845 6 98-99 1.34726845886712 6 100-101 1.4222451290461358 7 1 -0.09648573091961765 7 2 -0.18929782287492714 7 3 -0.16626379926039192 7 4 -0.16617251049457593 7 5 -0.2030303388293433 7 6 -0.24798230773362917 7 7 -0.2099965765787104 7 8 -0.2744601201441199 7 9 -0.26380482990828114 7 10-11 -0.3076818899268403 7 12-13 -0.3039477960296679 7 14-15 -0.27462142241350307 7 16-17 -0.17612151979343338 7 18-19 -0.24664445975545135 7 20-21 -0.3332692136950399 7 22-23 -0.21868097494200356 7 24-25 -0.18972697103176017 7 26-27 0.10469339087755003 7 28-29 0.08271289165645612 7 30-31 0.1024021682748355 7 32-33 0.012811700225967115 7 34-35 -0.02005159347383767 7 36-37 -0.07016350653269399 7 38-39 -0.18998965976923188 7 40-41 -0.16811342497349813 7 42-43 -0.11553523655994269 7 44-45 -0.06517519984517861 7 46-47 0.046520607498322875 7 48-49 0.08206195574062747 7 50-51 0.023168629885688574 7 52-53 -0.007957369906556266 7 54-55 0.04905970520017178 7 56-57 0.11184817053700868 7 58-59 0.1758605624283902 7 60-61 0.2349393017922239 7 62-63 0.28861175386676763 7 64-65 0.3374589791251097 7 66-67 0.426281834582241 7 68-69 0.4872528723542402 7 70-71 0.5193491237452506 7 72-73 0.6520167641548795 7 74-75 0.7148029917905347 7 76-77 0.8195976310874471 7 78-79 0.9274026204228498 7 80-81 1.0763280936216084 7 82-83 1.1686086341764117 7 84-85 1.1877489928135958 7 86-87 1.4176774117756068 7 88-89 1.5761794588697349 7 90-91 1.8748928844661776 7 92-93 2.048489578029457 7 94-95 2.1975794524596317 7 96-97 2.2058629288425955 7 98-99 2.418024409596015 7 100-101 2.4908028221861187 8 1 -0.45005372185770653 8 2 -0.6080379996499303 8 3 -0.6736135897050914 8 4 -0.5896418303152444 8 5 -0.4851537076901664 8 6 -0.6477372405090023 8 7 -0.5602385763073698 8 8 -0.5863502066595174 8 9 -0.570572296266576 8 10-11 -0.6941914548994959 8 12-13 -0.622797552537719 8 14-15 -0.6329289097625264 8 16-17 -0.6462814041461371 8 18-19 -0.5348918700251417 8 20-21 -0.45704265755158957 8 22-23 -0.2777279930494956 8 24-25 -0.441552577386787 8 26-27 -0.35588729506572037 8 28-29 -0.5042390150998983 8 30-31 -0.31920551270263786 8 32-33 -0.17938323855327098 8 34-35 -0.08832717848292759 8 36-37 -0.13727207538813957 8 38-39 -0.07806007050388075 8 40-41 -0.3572892200276101 8 42-43 -0.23049430778962687 8 44-45 -0.23452243406239148 8 46-47 -0.4438760387081686 8 48-49 -0.392356867442345 8 50-51 -0.6322980417613735 8 52-53 -0.6278007844515869 8 54-55 -0.5357286539650445 8 56-57 -0.591269816628575 8 58-59 -0.6196491302126717 8 60-61 -0.6827608092601594 8 62-63 -0.47174116723428483 8 64-65 -0.18496008835979438 8 66-67 0.03168317241063079 8 68-69 0.15919945282186632 8 70-71 0.12386595954401258 8 72-73 0.43107830838643224 8 74-75 0.14856639696842677 8 76-77 0.620329193886306 8 78-79 0.4579913200068795 8 80-81 0.371294147834508 8 82-83 1.0623458300571293 8 84-85 1.1791144847628132 8 86-87 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-0.4726308801556911 65 26-27 -1.1298102876647746 65 28-29 -1.0084666254415353 65 30-31 -0.7325049064521068 65 32-33 -0.83857044247533 65 34-35 -0.8106609036804997 65 36-37 -0.48664540657525635 65 38-39 -0.17687792487431153 65 40-41 -0.061479425331263826 65 42-43 0.014194843190111328 65 44-45 0.07742006216449226 65 46-47 0.15047948184231785 65 48-49 0.12631873785942105 65 50-51 0.12156474534467776 65 52-53 0.09140721679073494 65 54-55 -0.158258963460149 65 56-57 -0.08404995334844756 65 58-59 -0.03900658114419997 65 60-61 -0.07313236233524378 65 62-63 -0.024705065694568873 65 64-65 0.036407331668755205 65 66-67 0.0790487915608864 65 68-69 0.08050106908238064 65 70-71 0.10326483885732785 65 72-73 0.05153265497604309 65 74-75 0.08165477906234031 65 76-77 0.09756235742501929 65 78-79 0.022936575136728976 65 80-81 0.05733600651766224 65 82-83 0.018666242406432332 65 84-85 0.037943510021854365 65 86-87 0.02139122917635561 65 88-89 0.041779281387331224 65 90-91 0.001084491065693527 65 92-93 -0.08061714301271294 65 94-95 -0.007455057571966961 65 96-97 -0.05727301853427136 65 98-99 -0.08501269306730563 65 100-101 -0.17503611004643105 66 1 -0.018847025778917725 66 2 -0.05614769281797294 66 3 -0.03245359027619088 66 4 -0.07220903042378524 66 5 -0.04706579991419346 66 6 -0.07838322516519725 66 7 -0.08888641190008428 66 8 -0.09410233405004931 66 9 -0.16975646663383515 66 10-11 -0.15410906395334223 66 12-13 -0.15452487706397733 66 14-15 -0.18137594038094562 66 16-17 -0.09989229537317357 66 18-19 -0.08020646050328395 66 20-21 -0.03690366089839614 66 22-23 0.037986446847220634 66 24-25 0.06582288484280241 66 26-27 0.1633548764852577 66 28-29 0.1958872904786375 66 30-31 0.19335805265701822 66 32-33 0.16638788267196958 66 34-35 0.15795796847638144 66 36-37 -0.05519881646230829 66 38-39 -0.09140081871836259 66 40-41 -0.01723719563736381 66 42-43 -0.010077785654118543 66 44-45 -0.012593238793272121 66 46-47 -0.05865882212747664 66 48-49 -0.05042179497483801 66 50-51 -0.023819422335442653 66 52-53 -0.05215625901448817 66 54-55 -0.0582902596428454 66 56-57 0.052741875858401954 66 58-59 0.016308979844581017 66 60-61 0.021397201831064194 66 62-63 0.04628862340185691 66 64-65 0.03427921504788145 66 66-67 -0.03256416981197674 66 68-69 -0.03167861151311513 66 70-71 -0.052142484314060766 66 72-73 -0.07439091563831823 66 74-75 -0.09667612220341937 66 76-77 -0.13398729905729567 66 78-79 -0.09773530157618993 66 80-81 -0.05748578410864624 66 82-83 -0.11353974981856041 66 84-85 -0.1507993737910951 66 86-87 -0.1151536125017607 66 88-89 0.07981535008099527 66 90-91 0.18471688612691395 66 92-93 0.23573028477234104 66 94-95 0.29721508641108585 66 96-97 0.34299686724686396 66 98-99 0.3328614433518382 66 100-101 0.3268827828971972 67 1 -0.08412519447845312 67 2 -0.1279519924760777 67 3 -0.09491568239464954 67 4 -0.10959518317454808 67 5 -0.18394169151688544 67 6 -0.14680251563968483 67 7 -0.16470505483879805 67 8 -0.1926670399136441 67 9 -0.17201426061878777 67 10-11 -0.1698111464135863 67 12-13 -0.16780767174095956 67 14-15 -0.16507977695984266 67 16-17 -0.15828610947088606 67 18-19 -0.15046892253050714 67 20-21 -0.14602760013280403 67 22-23 -0.17084403964519623 67 24-25 -0.11430481586656782 67 26-27 -0.19662093844171835 67 28-29 -0.11526356098765689 67 30-31 -0.0014827414441498377 67 32-33 -0.02846874648849962 67 34-35 -0.1453135728156738 67 36-37 -0.15971713110236152 67 38-39 -0.1218243608427727 67 40-41 -0.11295242218788104 67 42-43 -0.08717772383418776 67 44-45 -0.11181628010035638 67 46-47 -0.0777888726375835 67 48-49 -0.0601102947178731 67 50-51 -0.05254534657747456 67 52-53 -0.0816624728521731 67 54-55 -0.07389409257037727 67 56-57 0.02087782037791186 67 58-59 0.02975872437190219 67 60-61 -0.031921535096856246 67 62-63 0.050798963436292865 67 64-65 0.05149640716201276 67 66-67 0.05322995917465789 67 68-69 0.11820313943586314 67 70-71 0.1252060494665912 67 72-73 0.12719736963288142 67 74-75 0.1742578504277219 67 76-77 0.2315739111259063 67 78-79 0.2390908694046523 67 80-81 0.369375950675348 67 82-83 0.4649953349483944 67 84-85 0.46649899332309985 67 86-87 0.7052821148230244 67 88-89 0.7939944391717653 67 90-91 1.0309942064848734 67 92-93 1.126856757281729 67 94-95 1.237726365094538 67 96-97 1.3185186752519407 67 98-99 1.3293297538046502 67 100-101 1.2752590012975382 68 1 -0.37952444104308114 68 2 -0.5770829551487751 68 3 -0.4620515821895381 68 4 -0.4592612957241471 68 5 -0.3984753049471905 68 6 -0.4467439325078857 68 7 -0.4799055349092143 68 8 -0.48895405035145956 68 9 -0.44847115727582576 68 10-11 -0.4614532475258386 68 12-13 -0.439499532496356 68 14-15 -0.5275033196106307 68 16-17 -0.4134355612851337 68 18-19 -0.5152625379023377 68 20-21 -0.35059093101002503 68 22-23 -0.24348010493466177 68 24-25 -0.26248866818497163 68 26-27 -0.2192358148134801 68 28-29 -0.3065069216683085 68 30-31 -0.26130642619408206 68 32-33 -0.0520204399091142 68 34-35 -0.04072605424326525 68 36-37 -0.12381902594505334 68 38-39 -0.13953177924210536 68 40-41 -0.14755689978996855 68 42-43 -0.2640268196216482 68 44-45 -0.21045744340097627 68 46-47 -0.26795553723679433 68 48-49 -0.36240407382469186 68 50-51 -0.43495084456056077 68 52-53 -0.4902979626934254 68 54-55 -0.6146145235237199 68 56-57 -0.2529573225617767 68 58-59 -0.2468625984440891 68 60-61 -0.3271875678363898 68 62-63 -0.009370157022033254 68 64-65 -0.0625547922823344 68 66-67 0.135612681080012 68 68-69 0.1955323315387858 68 70-71 0.3123773776732506 68 72-73 0.2855543154449265 68 74-75 0.37878263787972344 68 76-77 0.4743203002697669 68 78-79 0.675813944202698 68 80-81 0.8736349713674123 68 82-83 1.0466149529609545 68 84-85 1.1404589154729585 68 86-87 1.516049307366174 68 88-89 1.5741100767531755 68 90-91 1.8408756371257589 68 92-93 2.030302636242528 68 94-95 2.3207860767922135 68 96-97 2.455267353144613 68 98-99 2.4020639548351 68 100-101 2.3735574916349087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 585973.0 3 91192.0 4 137568.0 5 161570.0 6 214391.0 7 263153.0 8 409675.0 9 472459.0 10 681143.0 11 653929.0 12 743316.0 13 467878.0 14 514381.0 15 437211.0 16 461243.0 17 489514.0 18 535720.0 19 611964.0 20 692357.0 21 809558.0 22 917560.0 23 1060794.0 24 1201495.0 25 1382588.0 26 1595363.0 27 1847183.0 28 2164023.0 29 2551396.0 30 3074625.0 31 3754847.0 32 4703718.0 33 6017612.0 34 7832891.0 35 1.0411303E7 36 1.4537474E7 37 2.1588061E7 38 1.8408322E7 39 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.74145848289459 11.12944555946741 12.781452313021447 39.347643644616554 2 21.928572739952916 17.487229478113285 38.94896285505265 21.635234926881147 3 26.499561500673984 17.85903245268404 27.0181186141677 28.623287432474275 4 29.10525628995792 22.798300251986465 26.16684583147683 21.929597626578786 5 24.830818981573298 24.721288718760764 32.13183694456129 18.316055355104645 6 21.316169936042964 28.310245056445364 30.235423755327396 20.138161252184272 7 15.9534330283692 15.289699242202639 48.63619420949566 20.12067351993249 8 20.35279087505151 20.55637293496569 35.4627756135093 23.628060576473498 9 19.382257490824607 20.002698714670906 36.811855874234425 23.80318792027007 10-11 22.48923867449875 27.662173817844653 28.234705485550933 21.613882022105663 12-13 20.2609983185596 21.943831817429764 34.99383086906443 22.801338994946203 14-15 20.540526981309736 23.023098178989514 34.18582606623699 22.25054877346376 16-17 20.951911943784648 23.493404255114847 32.928697456056014 22.625986345044485 18-19 21.08647590966376 23.725537202378113 32.74897921369844 22.439007674259678 20-21 21.038263216210193 23.69603311651032 32.44111821964201 22.82458544763748 22-23 20.93500287356842 24.08851711800991 32.08342905115815 22.893050957263522 24-25 20.907354259731672 23.950606260807035 32.253385480009285 22.888653999452004 26-27 20.515774832624086 24.22583160204229 32.10153616094689 23.156857404386734 28-29 20.809563152885342 24.6658136056528 31.425949613174332 23.09867362828753 30-31 20.70461597244192 24.898941941230827 31.28233952122163 23.11410256510562 32-33 21.046986193992133 25.380789172045816 30.541210124020523 23.03101450994153 34-35 21.518867301880455 25.90239604840893 29.829872067512998 22.748864582197616 36-37 22.070586899040215 26.205639684693406 29.44045619825342 22.283317218012964 38-39 22.825301739101427 26.876985186496487 28.50211453322766 21.795598541174417 40-41 23.47327144950268 27.079693647342896 27.869543571740742 21.577491331413682 42-43 24.588833782498142 27.23244237156796 27.020428284414173 21.158295561519726 44-45 25.2595811512213 27.284679862437944 26.54013548906653 20.91560349727423 46-47 26.089463319784628 27.200496765320146 25.982197553561036 20.727842361334183 48-49 27.32839237777156 26.822319227933946 25.210567065575045 20.63872132871945 50-51 28.16439861331897 26.558061811564244 25.179808052243203 20.097731522873584 52-53 29.367986583176766 26.567377772360086 24.547197421950706 19.51743822251244 54-55 30.513377349720354 26.706480460538707 23.89542437193709 18.884717817803846 56-57 31.38138656949378 27.25778852784302 23.409236424940225 17.95158847772297 58-59 32.47013265205933 27.311171533745227 22.98699119286143 17.23170462133401 60-61 33.219284669787555 27.900572043601812 22.453566728385294 16.426576558225335 62-63 33.700241571552986 28.394086898211107 22.311143043631944 15.594528486603965 64-65 34.13489387420899 28.619420682169448 22.28715956036392 14.95852588325764 66-67 34.53016225215058 28.748989503011202 22.179146130188933 14.541702114649285 68-69 34.68107095806435 28.39860757402218 22.669661703374963 14.250659764538506 70-71 35.15011843211737 28.141604959665766 22.773794513525743 13.93448209469112 72-73 35.401477413859546 27.67763478128226 22.954924169681146 13.96596363517705 74-75 35.561729150384274 26.81842186173087 23.282107841321285 14.337741146563571 76-77 36.11250249092549 25.7166028347839 23.383062848208358 14.787831826082254 78-79 36.296954989149455 24.72522799249433 23.709868688619366 15.26794832973685 80-81 36.42836141358289 23.836516757104288 23.871791005796155 15.86333082351666 82-83 36.07512272947302 23.206664288173947 24.198818600938136 16.5193943814149 84-85 35.434226354039986 22.777782213795124 24.633872468099543 17.154118964065347 86-87 34.73967546689236 22.728151269919213 24.856240590964866 17.675932672223567 88-89 33.64691447391119 23.456051795745754 25.284041457523017 17.612992272820044 90-91 32.427725272880366 24.67223638159637 25.298788298625567 17.601250046897697 92-93 31.052946510415897 26.237892452275126 25.367576365036143 17.341584672272838 94-95 29.54802308642215 28.398003921747982 24.99977736691173 17.05419562491814 96-97 28.11949575701358 30.77512572108006 24.426011112608883 16.679367409297473 98-99 26.635487552024355 33.40821799016155 23.727193549382587 16.2291009084315 100-101 25.218627429434427 36.16409714145735 22.913195783527303 15.704079645580924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1246.0 1 1141.0 2 1081.5 3 1438.0 4 2083.0 5 3366.5 6 4583.0 7 5402.5 8 6683.0 9 8013.0 10 9347.0 11 11078.5 12 12986.0 13 14823.0 14 17209.0 15 20109.5 16 23349.5 17 27727.0 18 33686.5 19 39867.5 20 46553.0 21 55533.0 22 65427.5 23 77248.5 24 92545.5 25 111290.0 26 134235.0 27 164437.5 28 198816.0 29 240910.5 30 297367.0 31 365868.5 32 458128.0 33 572721.0 34 697065.0 35 852139.5 36 1059981.0 37 1329395.0 38 1681883.0 39 2014475.0 40 2382043.5 41 2923825.5 42 3604239.0 43 4434082.5 44 5390229.5 45 6338227.0 46 7190685.0 47 7852711.0 48 8164515.0 49 8064202.5 50 7663631.0 51 7083528.0 52 6356881.5 53 5517424.0 54 4616145.5 55 3721150.5 56 2880467.5 57 2140244.5 58 1543077.5 59 1095462.0 60 768804.5 61 537033.0 62 376094.5 63 274166.0 64 202353.0 65 141351.5 66 95744.0 67 66267.0 68 49474.0 69 38231.5 70 30250.5 71 25427.0 72 22626.0 73 22985.5 74 21979.0 75 16672.5 76 12959.0 77 10894.0 78 9423.5 79 8189.5 80 6897.0 81 5719.0 82 4660.0 83 3564.5 84 2510.5 85 1573.5 86 992.0 87 724.5 88 573.0 89 433.5 90 329.5 91 290.5 92 250.5 93 198.5 94 187.0 95 150.0 96 104.0 97 73.5 98 43.0 99 40.0 100 50.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0648671740041562 2 0.006133332878156288 3 0.001071266287603758 4 6.027539775895004E-4 5 5.654152356149296E-4 6 6.0453201292162286E-5 7 1.8047058621042565E-4 8 0.0020269602786195592 9 4.6851231001425773E-4 10-11 5.165192639815631E-4 12-13 6.049765217546535E-4 14-15 0.00376143374510498 16-17 0.00833187356632566 18-19 0.0016975792333438805 20-21 0.0013890901032206407 22-23 0.002202541267666648 24-25 0.0038289990877256315 26-27 0.006176894743793287 28-29 0.007777126542703465 30-31 0.006023983705230759 32-33 0.0013459727464166721 34-35 0.0 36-37 6.027539775895004E-4 38-39 0.002745286552797017 40-41 0.008942628702909712 42-43 0.0015926751487486577 44-45 0.003294254961589814 46-47 5.778614829397865E-6 48-49 5.689713062791744E-5 50-51 0.0032173549334755195 52-53 0.02584018748173513 54-55 0.035942984238854725 56-57 0.0033484850392195478 58-59 0.0023790112743797983 60-61 0.0012948542306181524 62-63 6.000869245913168E-4 64-65 9.596945705130762E-4 66-67 0.0028101848424194847 68-69 0.0032124653363121824 70-71 0.04416284157925667 72-73 0.17623930662880732 74-75 0.04911022489088731 76-77 0.00482736592671237 78-79 0.02248058972168982 80-81 0.01835154717166853 82-83 0.014329631250407614 84-85 0.07386092122286443 86-87 0.0794857359960337 88-89 0.04588486879841724 90-91 0.016147672377502788 92-93 0.014635008818699639 94-95 0.02034294674364564 96-97 0.018192857518276602 98-99 0.02240102264057734 100-101 0.02189961667691882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.12483704E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.197414067675677 #Duplication Level Percentage of deduplicated Percentage of total 1 55.889025385859725 14.08258914486345 2 17.78801124908174 8.96423769767171 3 9.150122325101835 6.916782629864228 4 4.8127965976915075 4.850801147821345 5 2.7312783071186284 3.441057521926417 6 1.7515268888171565 2.6480368960916176 7 1.1352655862035197 2.0024029936646848 8 0.8709786749937002 1.7557128254345429 9 0.6876562970007151 1.5594444406594423 >10 4.444145735369986 21.44599857984825 >50 0.4305695097620734 7.539678753218524 >100 0.2746598884484705 13.172896583730385 >500 0.021446234269506894 3.6833943927829402 >1k 0.011563204303991924 5.322945377291703 >5k 6.789725367190867E-4 1.1531091099671418 >10k+ 2.7514344075022624E-4 1.4609119051638324 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 156244 0.1389036762160677 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9558388653346622E-5 0.0 0.0 1.7780353321224202E-6 0.0 2 7.645551928126407E-5 6.22312366242847E-6 0.0 5.33410599636726E-6 0.0 3 1.4490987956797724E-4 6.22312366242847E-6 0.0 5.33410599636726E-6 8.890176660612101E-7 4 2.2225441651530252E-4 6.22312366242847E-6 0.0 7.112141328489681E-6 8.890176660612101E-7 5 2.83596635473526E-4 6.22312366242847E-6 0.0 8.890176660612101E-6 8.890176660612101E-7 6 3.5560706642448405E-4 6.22312366242847E-6 0.0 8.890176660612101E-6 8.890176660612101E-7 7 4.240614267111972E-4 6.22312366242847E-6 0.0 8.890176660612101E-6 8.890176660612101E-7 8 4.987389106603389E-4 6.22312366242847E-6 0.0 8.890176660612101E-6 8.890176660612101E-7 9 5.751944299416029E-4 6.22312366242847E-6 0.0 8.890176660612101E-6 8.890176660612101E-7 10-11 0.0019260567735216116 6.22312366242847E-6 4.4450883303060505E-7 8.890176660612101E-6 8.890176660612101E-7 12-13 0.002259882907127596 6.667632495459076E-6 8.890176660612101E-7 9.334685493642707E-6 8.890176660612101E-7 14-15 0.003316924912074375 7.112141328489681E-6 8.890176660612101E-7 1.155722965879573E-5 8.890176660612101E-7 16-17 0.0044873166694439585 7.556650161520286E-6 8.890176660612101E-7 1.689133565516299E-5 8.890176660612101E-7 18-19 0.005479460384768268 8.001158994550891E-6 8.890176660612101E-7 2.889307414698933E-5 8.890176660612101E-7 20-21 0.05937704540739519 8.001158994550891E-6 8.890176660612101E-7 3.378267131032598E-5 8.890176660612101E-7 22-23 0.07949996027869068 8.001158994550891E-6 8.890176660612101E-7 3.956128613972385E-5 8.890176660612101E-7 24-25 0.13514668755929304 8.001158994550891E-6 8.890176660612101E-7 4.533990096912172E-5 8.890176660612101E-7 26-27 0.37903979406652544 8.001158994550891E-6 8.890176660612101E-7 5.556360412882563E-5 8.890176660612101E-7 28-29 1.0560960901500898 8.001158994550891E-6 8.890176660612101E-7 6.712083378762135E-5 1.333526499091815E-6 30-31 2.226852344762758 8.001158994550891E-6 8.890176660612101E-7 7.823355461338647E-5 1.7780353321224202E-6 32-33 4.044056017216502 8.001158994550891E-6 8.890176660612101E-7 9.156881960430463E-5 1.7780353321224202E-6 34-35 6.346090363453892 8.001158994550891E-6 8.890176660612101E-7 9.77919432667331E-5 1.7780353321224202E-6 36-37 9.353820709887007 8.001158994550891E-6 8.890176660612101E-7 1.0445957576219218E-4 1.7780353321224202E-6 38-39 12.829354374745696 8.001158994550891E-6 8.890176660612101E-7 1.0490408459522279E-4 1.7780353321224202E-6 40-41 16.60221733096556 8.001158994550891E-6 8.890176660612101E-7 1.0890466409249822E-4 1.7780353321224202E-6 42-43 20.56660314102032 8.001158994550891E-6 8.890176660612101E-7 1.1823934958614095E-4 1.7780353321224202E-6 44-45 24.594315901972784 8.001158994550891E-6 8.890176660612101E-7 1.2179542025038578E-4 1.7780353321224202E-6 46-47 28.693382998838658 1.066821199273452E-5 8.890176660612101E-7 1.240179644155388E-4 1.7780353321224202E-6 48-49 32.76467096069312 1.3779773823948758E-5 8.890176660612101E-7 1.3157461457705908E-4 1.7780353321224202E-6 50-51 36.754879622385126 1.4224282656979362E-5 8.890176660612101E-7 1.3157461457705908E-4 1.7780353321224202E-6 52-53 40.58271409696822 1.4668791490009966E-5 8.890176660612101E-7 1.342416675752427E-4 1.7780353321224202E-6 54-55 44.250942785454505 1.5113300323040571E-5 8.890176660612101E-7 1.404647912376712E-4 1.7780353321224202E-6 56-57 47.74915929155391 1.5113300323040571E-5 8.890176660612101E-7 1.4313184423585482E-4 1.7780353321224202E-6 58-59 51.038682456616115 1.866937098728541E-5 8.890176660612101E-7 1.4535438840100785E-4 1.7780353321224202E-6 60-61 54.16626305264627 2.266995048456086E-5 8.890176660612101E-7 1.4668791490009966E-4 1.7780353321224202E-6 62-63 57.11911344953577 2.6670529981836304E-5 8.890176660612101E-7 1.493549678982833E-4 1.7780353321224202E-6 64-65 59.88134956864508 3.1115618312142354E-5 8.890176660612101E-7 1.5157751206343632E-4 1.7780353321224202E-6 66-67 62.46126816734271 4.2228339137907484E-5 8.890176660612101E-7 1.5646710922677298E-4 1.7780353321224202E-6 68-69 64.87165331966665 5.6008112961856235E-5 8.890176660612101E-7 1.582451445588954E-4 1.7780353321224202E-6 70-71 67.08832996822366 6.889886911974377E-5 8.890176660612101E-7 1.6269023288920144E-4 1.7780353321224202E-6 72-73 69.15738434431356 8.667922244096798E-5 8.890176660612101E-7 1.671353212195075E-4 1.7780353321224202E-6 74-75 71.09208770365528 1.053485934282534E-4 8.890176660612101E-7 1.671353212195075E-4 1.7780353321224202E-6 76-77 72.86945049391332 1.1868385841917154E-4 8.890176660612101E-7 1.671353212195075E-4 1.7780353321224202E-6 78-79 74.52000424879323 1.3557519407433454E-4 8.890176660612101E-7 1.680243388855687E-4 1.7780353321224202E-6 80-81 76.04393388396954 1.6135670639010963E-4 8.890176660612101E-7 1.6980237421769113E-4 1.7780353321224202E-6 82-83 77.45415327005946 1.8180411270951746E-4 8.890176660612101E-7 1.7291393604890534E-4 1.7780353321224202E-6 84-85 78.7528951749313 2.0002897486377227E-4 8.890176660612101E-7 1.7424746254799718E-4 1.7780353321224202E-6 86-87 79.93785926537413 2.2625499601257798E-4 8.890176660612101E-7 1.7513648021405838E-4 1.7780353321224202E-6 88-89 81.02466558178062 2.52036508328353E-4 8.890176660612101E-7 1.773590243792114E-4 1.7780353321224202E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 121480 0.0 61.042057 1 CGGCATT 20965 0.0 54.776752 1 GCGTGTT 146805 0.0 52.25242 2 CGTGTTT 215300 0.0 36.004593 3 CACGCGT 7105 0.0 33.035233 1 CGGGTGT 21945 0.0 30.83109 1 GGCGGAT 16970 0.0 29.81826 1 CGGGGCT 16845 0.0 28.318954 1 GCGGATT 18580 0.0 28.231756 1 ACGCGTC 8240 0.0 27.948265 2 CGGGCGT 6495 0.0 27.8715 1 TGAACCG 18245 0.0 23.907207 12-13 GGCATTC 49250 0.0 23.129396 2 CGCGTCT 16325 0.0 23.122566 3 CCCCGCT 23250 0.0 23.031143 1 GTGTTTT 357360 0.0 22.78796 4 CGCGCGT 6795 0.0 22.23577 1 GAACCGA 13150 0.0 22.19165 14-15 CGGATTT 21585 0.0 22.174242 2 GCGGGCT 9195 0.0 22.064327 1 >>END_MODULE