FastQCFastQC Report
Fri 27 May 2016
SRR1233078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112483704
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1344510.11952931421959576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAT155350.031.9722391
GCGAATT76750.031.6824052
GGCGAAT88700.030.763161
CGGGGCT173500.029.5882471
CGGGGGT131700.029.4332751
GCGGATT183400.028.5882741
CGGCGGT28450.027.953371
CGGGCAT122750.026.10921
CGGATTT182550.025.417882
CGCGTGT87250.024.1244181
CGGGTGT162050.023.4505921
GTTTATT1088800.023.2813591
CGAATTT105000.022.6607573
TATACGC130750.022.0843587
CGGGCGT55400.021.9194871
ACTTAAG588600.021.9064946
CCGGCCT199450.021.846761
TACGCTA133950.021.5922379
CCGGGCT114300.020.8316561
CCGGGTT126750.020.7767281
Produced by FastQC (version 0.11.5)