FastQCFastQC Report
Fri 27 May 2016
SRR1233077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111879077
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT4537870.40560488356549457No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA1413310.12632478188928925No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1188100.10619501267426439No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1176870.10519125037114847No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1121750.10026450253964823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT316450.066.830061
GGCATTC492900.042.1468242
CACGCGT233250.038.9305461
ACGCGTC238850.038.91652
CGCGTGT1241250.034.5465471
GTCGGAC71100.033.264731
TGAACCG241100.033.1533212-13
GCGTGTT1341850.031.9646932
CGGGGCT162500.030.9504431
GCATTCC778200.030.4325643
CGCGTCT407150.029.5439413
GCGGATT148000.029.4902211
ATTCCTG725350.029.2068355
CGGGTGT152000.028.9016321
GAACCGA148650.027.38959314-15
GGCGGAT149400.026.8615061
GAACCGC159050.026.598714-15
CGGAGAA326050.025.9896492
ACCGCTC112900.025.6540116-17
GCGGGCT94500.025.027751
Produced by FastQC (version 0.11.5)