##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111879077 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.134546953761514 39.0 37.0 39.0 33.0 39.0 2 36.807740360603795 39.0 37.0 39.0 33.0 39.0 3 36.78449452170579 39.0 37.0 39.0 33.0 39.0 4 36.73623686580825 39.0 37.0 39.0 33.0 39.0 5 36.69061847015416 39.0 37.0 39.0 33.0 39.0 6 36.7654803587627 39.0 37.0 39.0 33.0 39.0 7 36.75584174688892 39.0 37.0 39.0 33.0 39.0 8 36.69079829823766 39.0 36.0 39.0 33.0 39.0 9 36.649508781700085 39.0 36.0 39.0 32.0 39.0 10-11 36.643308314922905 39.0 36.5 39.0 32.5 39.0 12-13 36.56064799765912 39.0 36.0 39.0 32.0 39.0 14-15 36.47426451328339 39.0 36.0 39.0 32.0 39.0 16-17 36.34512826737031 39.0 36.0 39.0 31.5 39.0 18-19 36.21822463730193 39.0 36.0 39.0 31.0 39.0 20-21 36.097923135350854 39.0 36.0 39.0 31.0 39.0 22-23 35.91924068161556 38.0 36.0 39.0 30.5 39.0 24-25 35.759890046286316 38.0 36.0 39.0 30.0 39.0 26-27 35.53789595082198 38.0 35.5 39.0 29.5 39.0 28-29 35.3191007868254 38.0 35.0 39.0 29.0 39.0 30-31 35.18973347447262 38.0 35.0 39.0 28.0 39.0 32-33 35.07669322298753 38.0 35.0 39.0 28.0 39.0 34-35 34.915753555063745 37.0 35.0 39.0 28.0 39.0 36-37 35.23135766484738 38.0 35.0 39.0 29.0 39.0 38-39 35.060797802255735 38.0 35.0 39.0 28.0 39.0 40-41 35.03685848695373 37.5 35.0 39.0 28.5 39.0 42-43 34.920528826851154 37.0 35.0 39.0 28.0 39.0 44-45 34.76651860919446 37.0 34.5 39.0 28.0 39.0 46-47 34.42727147275268 37.0 34.0 39.0 27.0 39.0 48-49 34.20161621462072 37.0 34.0 39.0 27.0 39.0 50-51 34.304231853825534 37.5 34.0 39.0 26.5 39.0 52-53 34.398929846373335 37.0 34.5 39.0 26.0 39.0 54-55 34.253324274385996 37.0 34.0 39.0 26.0 39.0 56-57 34.62713588082247 38.0 35.0 39.0 27.0 39.0 58-59 34.415091366905 37.5 34.5 39.0 26.5 39.0 60-61 34.28096597096524 37.0 34.5 39.0 26.0 39.0 62-63 34.23661138623802 37.0 34.0 39.0 26.0 39.0 64-65 34.03503959904853 37.0 34.0 39.0 25.5 39.0 66-67 33.92718372622971 37.0 34.0 39.0 25.0 39.0 68-69 33.67491649488671 37.0 33.5 39.0 24.5 39.0 70-71 33.39945228990403 37.0 33.0 39.0 23.5 39.0 72-73 33.01673101039259 37.0 33.0 39.0 22.0 39.0 74-75 32.737290311216995 36.5 32.5 39.0 21.0 39.0 76-77 32.33579616052785 36.0 32.0 39.0 19.0 39.0 78-79 31.88502873061779 36.0 31.0 38.0 17.5 39.0 80-81 31.388429661428113 36.0 30.5 38.0 12.5 39.0 82-83 30.847275299741703 35.0 30.0 37.0 8.5 39.0 84-85 30.320631318758554 35.0 29.0 37.0 2.0 39.0 86-87 29.576898962975893 34.5 27.5 37.0 2.0 39.0 88-89 28.96552016602711 34.0 27.0 37.0 2.0 39.0 90-91 28.075609968609236 33.5 25.5 36.5 2.0 39.0 92-93 27.042353558208205 33.0 23.0 36.0 2.0 38.0 94-95 26.024159910614923 32.0 20.0 36.0 2.0 37.0 96-97 24.479546287283007 31.5 2.0 36.0 2.0 37.0 98-99 23.382839550061718 30.5 2.0 35.0 2.0 37.0 100-101 22.53228095544621 29.5 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.29735458779522617 1 2 0.4617854211708874 1 3 0.46000336916377904 1 4 0.4629260662168022 1 5 0.4406486637560576 1 6 0.4595188532160748 1 7 0.4602146955019748 1 8 0.4903798788687439 1 9 0.5051139022781328 1 10-11 0.4488723172262965 1 12-13 0.47619259350724263 1 14-15 0.4634344704956277 1 16-17 0.2670661255203015 1 18-19 0.265199693566359 1 20-21 0.2003890956372274 1 22-23 0.0034351202359133026 1 24-25 0.045274433340424025 1 26-27 -0.45555192292036395 1 28-29 -0.5066871992380868 1 30-31 -0.506453871103794 1 32-33 -0.28830177917867417 1 34-35 -0.06993629260385603 1 36-37 0.11616943616999009 1 38-39 -0.016847815917017783 1 40-41 0.20799873777021105 1 42-43 0.10439612453016878 1 44-45 0.14376288974885654 1 46-47 0.1833432410602498 1 48-49 -0.06822192650105308 1 50-51 0.08145226688866103 1 52-53 -0.05991386263686138 1 54-55 0.03304513872286918 1 56-57 -0.34388118364841347 1 58-59 -0.6394326833404165 1 60-61 -0.39808003466442443 1 62-63 -0.19863688710695016 1 64-65 -0.1907056807787697 1 66-67 -0.12261400613885343 1 68-69 -0.0786598755976442 1 70-71 -0.16229101984369265 1 72-73 -0.058527282143003845 1 74-75 -0.1695361500778816 1 76-77 -0.2949902260137307 1 78-79 -0.34284732781257077 1 80-81 -0.4968123716172812 1 82-83 -0.6913608248639598 1 84-85 -0.8354871154432928 1 86-87 -1.1333895714326623 1 88-89 -1.1702955083960696 1 90-91 -1.395674844608756 1 92-93 -1.7240874472481806 1 94-95 -1.802413006246109 1 96-97 -1.8631266639718689 1 98-99 -1.8949436790094722 1 100-101 -1.9339908529260015 2 1 0.14148534426622916 2 2 0.13349915485785147 2 3 0.11945136525231703 2 4 0.19726833361524854 2 5 0.21425061528869094 2 6 0.23901674114596005 2 7 0.23335221964355668 2 8 0.21557215264231644 2 9 0.2038587078202596 2 10-11 0.17604052123045477 2 12-13 0.11891428321999342 2 14-15 0.16841729481021162 2 16-17 0.21395600565342932 2 18-19 0.23336025963721596 2 20-21 0.2262328404178362 2 22-23 0.20580454419940253 2 24-25 0.17674643714964589 2 26-27 0.37086595914744436 2 28-29 0.40687845560526625 2 30-31 0.4229789199430769 2 32-33 0.3547106988620641 2 34-35 0.3362480249078388 2 36-37 0.2203111134260709 2 38-39 0.2377804576150382 2 40-41 0.16680789787561423 2 42-43 0.14043657953862976 2 44-45 0.14619965517609756 2 46-47 0.17275380960361275 2 48-49 0.12955061716137095 2 50-51 0.19115920924527074 2 52-53 0.19341234078197544 2 54-55 0.24403000254423546 2 56-57 0.15719265602581345 2 58-59 0.17324449640430117 2 60-61 0.13577718573737485 2 62-63 0.11683187726031719 2 64-65 0.060355796315981536 2 66-67 -0.019667590671481605 2 68-69 -0.03774565577192135 2 70-71 -0.08658615152018001 2 72-73 -0.055959115374470514 2 74-75 -0.15693271574254197 2 76-77 -0.24315155411392197 2 78-79 -0.19085364263953153 2 80-81 -0.15119125482319618 2 82-83 -0.25215018302545644 2 84-85 -0.34576187573200556 2 86-87 -0.36644223649554064 2 88-89 -0.4558602226546782 2 90-91 -0.5243054102857521 2 92-93 -0.6940994535352267 2 94-95 -0.7725761665726516 2 96-97 -0.9064018540780552 2 98-99 -0.9553268293417858 2 100-101 -0.9399938281871485 3 1 0.1621070732813834 3 2 0.22106990038947316 3 3 0.20849136829880166 3 4 0.2041336182162894 3 5 0.21002078900377796 3 6 0.17657064379589826 3 7 0.2058085437309103 3 8 0.20576533720962686 3 9 0.20664797645370925 3 10-11 0.17031373015791473 3 12-13 0.12403993266696745 3 14-15 0.1857992338972565 3 16-17 0.25039731466853254 3 18-19 0.2121409705674111 3 20-21 0.2859285096375359 3 22-23 0.305643926872726 3 24-25 0.34353954020407684 3 26-27 0.42428098300240435 3 28-29 0.45744725350424176 3 30-31 0.39124979348750344 3 32-33 0.35822395238797355 3 34-35 0.29612622118032306 3 36-37 0.3032585485323196 3 38-39 0.31232775085607045 3 40-41 0.2959147200927248 3 42-43 0.2863748408898985 3 44-45 0.2541302695230456 3 46-47 0.22709139530750377 3 48-49 0.21230196274325408 3 50-51 0.24971362884085835 3 52-53 0.2763584896592306 3 54-55 0.259601245604415 3 56-57 0.2857978262886931 3 58-59 0.30796134476575077 3 60-61 0.23464301478432503 3 62-63 0.21579554286154945 3 64-65 0.2178593424012476 3 66-67 0.16304971002156776 3 68-69 0.07994744674504517 3 70-71 0.11781715121782099 3 72-73 0.21048591846169273 3 74-75 0.1541668867926802 3 76-77 0.10894932760389509 3 78-79 0.10307808944926222 3 80-81 0.20000478474709382 3 82-83 0.16682469959963342 3 84-85 0.08157664533790054 3 86-87 0.1924210730933673 3 88-89 0.14199046797381243 3 90-91 0.17441170888972124 3 92-93 0.21707657895166932 3 94-95 0.16600145686279433 3 96-97 0.11591181619098734 3 98-99 0.059268504398914956 3 100-101 0.022285802964304935 4 1 0.08970023888554124 4 2 0.10904908714903172 4 3 0.03154174902964968 4 4 -0.03748863853888906 4 5 0.07195227877548405 4 6 0.023192454407350738 4 7 0.038839041915203154 4 8 0.03386815648137542 4 9 0.03579203647784368 4 10-11 0.07056237672424714 4 12-13 0.07061192302236208 4 14-15 0.08728407990941633 4 16-17 0.060644808691449725 4 18-19 0.04353034165462333 4 20-21 0.08379508571775318 4 22-23 0.19468134526017877 4 24-25 0.21270214332809445 4 26-27 0.3231625053780576 4 28-29 0.3493052381766262 4 30-31 0.25938094620630636 4 32-33 0.22071138892154352 4 34-35 0.1594724662918381 4 36-37 0.008659483667358359 4 38-39 0.15222709532872614 4 40-41 0.04964423664540618 4 42-43 0.08451928124102892 4 44-45 0.10214806554841971 4 46-47 0.1296122043402974 4 48-49 0.13059254800000986 4 50-51 0.13022131960709515 4 52-53 0.14093273155823738 4 54-55 0.19114266626277754 4 56-57 0.16488214492951414 4 58-59 0.1804683448681672 4 60-61 0.13361052384156125 4 62-63 0.16866219399140903 4 64-65 0.14057420260532894 4 66-67 0.004030818833406613 4 68-69 0.18597828455241228 4 70-71 0.22165488678329126 4 72-73 0.30047713206465687 4 74-75 0.29041944782684226 4 76-77 0.3238401379447211 4 78-79 0.3114170012944797 4 80-81 0.4282912121156315 4 82-83 0.324182494880386 4 84-85 0.450734237486099 4 86-87 0.5469850491575201 4 88-89 0.5330478962996423 4 90-91 0.5410402664213514 4 92-93 0.4866665674253845 4 94-95 0.5470730770858836 4 96-97 0.5273207740469203 4 98-99 0.46471294856568335 4 100-101 0.5157912037210401 5 1 0.04049235777025473 5 2 -0.05743366988873788 5 3 -0.03105313552389788 5 4 -0.10474464010709994 5 5 -0.023840985749451704 5 6 -0.016433341483455877 5 7 -0.026251703051677566 5 8 -0.013588903173584299 5 9 -0.08592207351540537 5 10-11 -0.055937209781205866 5 12-13 -0.05915122518212712 5 14-15 -0.061498801001420134 5 16-17 -0.14237033127565724 5 18-19 -0.22115888770802172 5 20-21 -0.06493507158720746 5 22-23 -0.09414674158090719 5 24-25 -0.287051485528103 5 26-27 -0.3739577052248322 5 28-29 -0.23461352347453612 5 30-31 -0.32988935176976497 5 32-33 -0.3353966665776795 5 34-35 -0.5591797202287268 5 36-37 -0.43495303655092954 5 38-39 -0.3977856984220054 5 40-41 -0.16887733760822954 5 42-43 -0.03694885212654242 5 44-45 -0.10568295591141919 5 46-47 -0.1156767498797251 5 48-49 -0.008910387153164834 5 50-51 0.015229797559015879 5 52-53 0.06664529825962973 5 54-55 0.11459824929807638 5 56-57 -0.03224641113973803 5 58-59 0.1354335124900672 5 60-61 0.13266108736108606 5 62-63 0.1179109844516617 5 64-65 0.1562469113632119 5 66-67 0.2703843411963902 5 68-69 0.2073456343579565 5 70-71 0.31159353820652314 5 72-73 0.42144885046309355 5 74-75 0.44489603107174247 5 76-77 0.4721435698747527 5 78-79 0.49876001068749787 5 80-81 0.5956010503973381 5 82-83 0.6134284716129486 5 84-85 0.6805223529969773 5 86-87 0.9028696967885672 5 88-89 0.9216834373237575 5 90-91 0.9900279163234735 5 92-93 1.0480031493222945 5 94-95 1.0914506659391314 5 96-97 1.1560668747281468 5 98-99 1.2461163796605703 5 100-101 1.238908860549735 6 1 -0.04555287188906476 6 2 -0.11304953117774375 6 3 -0.12092228964569074 6 4 -0.14102077742423802 6 5 -0.15102588008116413 6 6 -0.13903475494962692 6 7 -0.10913449493504146 6 8 -0.1829579916589381 6 9 -0.1104440314652777 6 10-11 -0.12772095988820809 6 12-13 -0.18292353392330796 6 14-15 -0.18299016259302903 6 16-17 -0.03146150997587682 6 18-19 -0.06608036785149096 6 20-21 -0.04498427403068206 6 22-23 -0.030266779631062946 6 24-25 0.004962814132468907 6 26-27 0.21978933211623541 6 28-29 0.2480896266488628 6 30-31 0.19649487785403608 6 32-33 0.16636050684272874 6 34-35 0.1047968941559887 6 36-37 0.07601993638069615 6 38-39 0.12303766371892522 6 40-41 -0.01171732088123889 6 42-43 0.06020065403592412 6 44-45 0.03340289955750819 6 46-47 0.0252668156559821 6 48-49 0.018736026728134902 6 50-51 0.020603426009593306 6 52-53 0.0010082793256458444 6 54-55 0.059336755851013834 6 56-57 0.17119923428050043 6 58-59 0.17505443331978654 6 60-61 0.17294780856482106 6 62-63 0.20782856949349338 6 64-65 0.1544814360626603 6 66-67 0.08107498467976626 6 68-69 0.07237147090383189 6 70-71 0.13304635309494017 6 72-73 0.2709710149360731 6 74-75 0.34695661467248584 6 76-77 0.31852260357030815 6 78-79 0.47814669364807827 6 80-81 0.5120240379296277 6 82-83 0.6232812543813182 6 84-85 0.6654637153121392 6 86-87 0.6746465792618679 6 88-89 0.8747829642604259 6 90-91 1.0011776647110473 6 92-93 1.1161153200589986 6 94-95 1.1931152411717 6 96-97 1.354866778336941 6 98-99 1.4665789314693072 6 100-101 1.521124078253962 7 1 -0.14549345816271853 7 2 -0.20662786704968994 7 3 -0.2781449536403926 7 4 -0.2438485571055793 7 5 -0.21796145058618066 7 6 -0.24896582614209706 7 7 -0.21489708946999997 7 8 -0.28749371074661667 7 9 -0.24501368439926097 7 10-11 -0.20890859013093888 7 12-13 -0.25443033853326114 7 14-15 -0.20134653560502613 7 16-17 -0.17153099756098555 7 18-19 -0.16514811410441155 7 20-21 -0.10851011325450344 7 22-23 -0.022297874331329126 7 24-25 0.031322975359266536 7 26-27 0.12209831230408241 7 28-29 0.14510126388295674 7 30-31 0.184649840777368 7 32-33 0.10776419656318836 7 34-35 0.079321474084729 7 36-37 0.005952226639372782 7 38-39 0.04687170458156231 7 40-41 -0.02796009580620762 7 42-43 -0.05532292851880527 7 44-45 -0.05988995048411283 7 46-47 0.061185441086884396 7 48-49 0.041988419870136795 7 50-51 0.03810694862382036 7 52-53 0.011138048522113309 7 54-55 0.03294555105057384 7 56-57 0.1253820164442132 7 58-59 0.1637271910981397 7 60-61 0.2184401491173844 7 62-63 0.27455243629010795 7 64-65 0.2792251205716738 7 66-67 0.37481306777279144 7 68-69 0.34732814020066627 7 70-71 0.48593646184588835 7 72-73 0.6269363535296435 7 74-75 0.6777668022576293 7 76-77 0.8415353829448904 7 78-79 0.9508027453388124 7 80-81 1.1725873573949386 7 82-83 1.2547245476012847 7 84-85 1.4031207622311008 7 86-87 1.7700512322329196 7 88-89 1.8497267691336887 7 90-91 2.015600342603907 7 92-93 2.3444813080034343 7 94-95 2.413083333473704 7 96-97 2.622068695189 7 98-99 2.7720568756572277 7 100-101 2.6347830106085155 8 1 -0.43657780292191006 8 2 -0.6430614961905121 8 3 -0.6044035831319476 8 4 -0.5546298775860237 8 5 -0.7382657362823082 8 6 -0.6800974859381697 8 7 -0.5794224811080824 8 8 -0.5994828193722554 8 9 -0.597052735134973 8 10-11 -0.5441176624603301 8 12-13 -0.4977853161170529 8 14-15 -0.4586531293621121 8 16-17 -0.3427488834065997 8 18-19 -0.30292142460563554 8 20-21 -0.28875578381261136 8 22-23 -0.25569570139883524 8 24-25 -0.5809164494326211 8 26-27 -0.5508167078122739 8 28-29 -0.5376526761211693 8 30-31 -0.32915962224535633 8 32-33 -0.13416437928236036 8 34-35 -0.20455550780543064 8 36-37 -0.13257214599230593 8 38-39 -0.31675140326883877 8 40-41 -0.20554052260321498 8 42-43 -0.2803552227612087 8 44-45 -0.29464328642998794 8 46-47 -0.2701897619248115 8 48-49 -0.2661562006550824 8 50-51 -0.45120828377990563 8 52-53 -0.6227248289717409 8 54-55 -0.6486454557473849 8 56-57 -0.30683103981741766 8 58-59 -0.5336630199306427 8 60-61 -0.12605161838521894 8 62-63 0.02968324209128781 8 64-65 0.2049481274537115 8 66-67 0.15812276912851786 8 68-69 0.007873922280367651 8 70-71 0.041021000150550435 8 72-73 0.18765490257377593 8 74-75 0.2588801653712167 8 76-77 0.5803039144878923 8 78-79 0.8786602599661677 8 80-81 1.2229365496577316 8 82-83 1.4091897755300167 8 84-85 1.639687456883106 8 86-87 1.8023405735015103 8 88-89 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-0.10572966479182355 65 28-29 -0.6168906949862389 65 30-31 -0.2560291080796304 65 32-33 -0.5406308530478512 65 34-35 -0.6843172161734472 65 36-37 -0.10343035719916571 65 38-39 -0.12344726723547694 65 40-41 0.04505016138738682 65 42-43 0.10713977072431646 65 44-45 0.1383650525141178 65 46-47 0.07684411324544982 65 48-49 0.13988807892089028 65 50-51 0.13862035386738825 65 52-53 0.09101595699110021 65 54-55 -0.1362386794935304 65 56-57 0.021237168208280366 65 58-59 0.057497330123126744 65 60-61 0.09151104787778763 65 62-63 -0.010742397628220601 65 64-65 0.06928235637813174 65 66-67 0.0752491086122049 65 68-69 0.09967581247124002 65 70-71 0.13743419934797174 65 72-73 0.14614212200958576 65 74-75 0.09418667316354146 65 76-77 0.12795488086531037 65 78-79 0.05434442016793284 65 80-81 0.076871540329158 65 82-83 0.09832263659284024 65 84-85 0.1255477809909813 65 86-87 0.09204934190924874 65 88-89 0.07902762461713664 65 90-91 0.11905166230739539 65 92-93 0.09948187113202422 65 94-95 0.11321346741998539 65 96-97 0.008628749350442888 65 98-99 0.025423671365221168 65 100-101 -0.008496306219264227 66 1 -0.08867469356614066 66 2 -0.17995978787605793 66 3 -0.1699533190781608 66 4 -0.11498400671032982 66 5 -0.15170112138211778 66 6 -0.20553728603743338 66 7 -0.0916465901091641 66 8 -0.1359770270471259 66 9 -0.11692337604589653 66 10-11 -0.09889623880879839 66 12-13 -0.09513835744618149 66 14-15 -0.08818223950315485 66 16-17 -0.05974893917871782 66 18-19 -0.019188790467929095 66 20-21 -0.07342434639355844 66 22-23 0.016753677126637 66 24-25 0.050134719302761255 66 26-27 0.1394051451345817 66 28-29 0.14321482425469867 66 30-31 0.1831508980775709 66 32-33 0.04484986764988719 66 34-35 0.025390723761454126 66 36-37 0.004351611790156085 66 38-39 0.06917042432014853 66 40-41 0.095396570661471 66 42-43 0.04926619459090631 66 44-45 0.09977872687422007 66 46-47 0.04838485321797492 66 48-49 0.0905344224338549 66 50-51 0.09650958353150685 66 52-53 0.1123258364020927 66 54-55 0.12629178309969546 66 56-57 0.1542471199514992 66 58-59 0.12821837784544954 66 60-61 0.08617707278076381 66 62-63 0.07636814562039973 66 64-65 0.07296772206255042 66 66-67 0.03833564844712356 66 68-69 0.056117971930660815 66 70-71 -0.003332191901620263 66 72-73 0.050011924869629354 66 74-75 0.04671985142935853 66 76-77 0.1492825227677983 66 78-79 0.10647381938208866 66 80-81 0.19868777902271262 66 82-83 0.25415811205499494 66 84-85 0.30099445288454163 66 86-87 0.3470484547645398 66 88-89 0.41162315558982954 66 90-91 0.4643972930922402 66 92-93 0.5265105773010355 66 94-95 0.5410397947608736 66 96-97 0.550199766166223 66 98-99 0.6189091778053495 66 100-101 0.5569909598324223 67 1 -0.06112441422745718 67 2 -0.09548249219653115 67 3 -0.06623574692395806 67 4 -0.07530259640179793 67 5 -0.06522762272643234 67 6 -0.08546426566148568 67 7 -0.053091771162264934 67 8 -0.05947434863160339 67 9 -0.07751345994257974 67 10-11 -0.11560550703746486 67 12-13 -0.11946435293076263 67 14-15 -0.17189229064618416 67 16-17 -0.11381349595500723 67 18-19 -0.1968141301896864 67 20-21 -0.2346421782063146 67 22-23 -0.1394087589127082 67 24-25 -0.10247197706094369 67 26-27 0.07736125614133016 67 28-29 0.04521277119388856 67 30-31 0.11129756722012019 67 32-33 -0.015206785851454185 67 34-35 -0.07378870711154661 67 36-37 -0.07627466989642784 67 38-39 -0.11394588379586423 67 40-41 -0.07355356119330025 67 42-43 -0.009455077925657918 67 44-45 0.019966619868654334 67 46-47 -0.05496292655717383 67 48-49 0.06706481891510663 67 50-51 0.011128341574888623 67 52-53 0.026217602094163794 67 54-55 0.04173712374060301 67 56-57 0.04108508196595295 67 58-59 0.06345184512539959 67 60-61 0.10155547178110425 67 62-63 0.08075004911729167 67 64-65 0.10664800168125055 67 66-67 0.21461701856767235 67 68-69 0.2948781501750446 67 70-71 0.32531094326296284 67 72-73 0.3533254132247663 67 74-75 0.3853271090787942 67 76-77 0.4720976466112674 67 78-79 0.5359144877810067 67 80-81 0.652329866248536 67 82-83 0.7831000987210643 67 84-85 0.8241378840540143 67 86-87 0.9924015693743193 67 88-89 1.1025296676219725 67 90-91 1.18560903784336 67 92-93 1.4225923063525698 67 94-95 1.50750164554114 67 96-97 1.532345358838473 67 98-99 1.547289251595732 67 100-101 1.47780037904419 68 1 -0.450217589953553 68 2 -0.5782884997078952 68 3 -0.5755736665167319 68 4 -0.5396391479879625 68 5 -0.5211309980460754 68 6 -0.5248816348779073 68 7 -0.5225760408267277 68 8 -0.6974371110528992 68 9 -0.5494606486030165 68 10-11 -0.574100527485605 68 12-13 -0.5834079911621615 68 14-15 -0.6560717412009751 68 16-17 -0.4764544132606119 68 18-19 -0.49193591927260627 68 20-21 -0.4025366998891897 68 22-23 -0.366722125570206 68 24-25 -0.3871057885523186 68 26-27 -0.35956835683357724 68 28-29 -0.3205707896131855 68 30-31 -0.54991448471214 68 32-33 -0.3880668034382708 68 34-35 -0.20207525634258872 68 36-37 -0.3212877101642775 68 38-39 -0.24631273843733226 68 40-41 -0.3484972064148053 68 42-43 -0.4369595573739957 68 44-45 -0.3097464217922976 68 46-47 -0.2949709615212228 68 48-49 -0.4602128498112208 68 50-51 -0.468251161213594 68 52-53 -0.5953112645331942 68 54-55 -0.5695859859969659 68 56-57 -0.2942602055601711 68 58-59 -0.3411747283275943 68 60-61 -0.25441113360666634 68 62-63 -0.12412633039344456 68 64-65 -0.1258240462241531 68 66-67 0.08221283304217764 68 68-69 0.14621503879875775 68 70-71 0.16696479931829344 68 72-73 0.06354016532191764 68 74-75 0.21639600401583436 68 76-77 0.47585083503489756 68 78-79 0.5117417993731763 68 80-81 0.5914117087233848 68 82-83 0.9552082408924889 68 84-85 1.1171985248729221 68 86-87 1.185629526514763 68 88-89 1.2678328494035114 68 90-91 1.8239621113806948 68 92-93 2.15889505059112 68 94-95 2.3786341125224446 68 96-97 2.655766792177225 68 98-99 2.6170508879086363 68 100-101 2.7662187536509464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 712373.0 3 95626.0 4 141701.0 5 158866.0 6 196262.0 7 204840.0 8 253314.0 9 260238.0 10 337765.0 11 341113.0 12 394432.0 13 317431.0 14 354454.0 15 355262.0 16 392606.0 17 445447.0 18 505841.0 19 599427.0 20 696085.0 21 824586.0 22 947464.0 23 1102287.0 24 1253139.0 25 1443121.0 26 1667383.0 27 1935147.0 28 2273295.0 29 2687112.0 30 3253737.0 31 4002249.0 32 5034209.0 33 6457754.0 34 8347719.0 35 1.09424E7 36 1.4985667E7 37 2.1539154E7 38 1.6419402E7 39 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.705965023272995 11.288476327131146 13.581224128843154 40.42433452075271 2 21.64385864608468 17.81474121507666 38.019848073616714 22.521552065221943 3 25.962718759793376 18.772433493549663 25.20463174180403 30.060216004852936 4 29.275229029775435 23.337459697999805 24.008626661577612 23.378684610647145 5 24.321638879801668 25.497819965840517 31.035348748827786 19.14519240553003 6 20.67715109999131 30.252072757035403 27.974646808409364 21.096129334563923 7 15.730994862957068 16.016540080465738 47.31547718543322 20.936987871143977 8 20.325207241613303 21.175281738303113 33.7048348985866 24.794676121496988 9 19.25157138129356 21.289147048264294 34.60022732964932 24.859054240792826 10-11 22.772860646828942 28.572768578682922 25.831192526433927 22.823178248054212 12-13 20.54157668654261 22.913594558189985 32.93931954376093 23.605509211506472 14-15 21.11452819335086 24.02651145597648 32.20595809781898 22.653002252853682 16-17 21.64818560386843 24.259326989123593 31.040627906288954 23.05185950071902 18-19 21.717191630970166 24.628051860733137 30.842148155896922 22.81260835239978 20-21 21.70525645336979 24.36396232966231 30.663389367714842 23.26739184925306 22-23 21.41939430017898 24.56957144945113 30.44476141664732 23.56627283372257 24-25 21.03757445071786 24.343549672217513 31.236888834622466 23.38198704244216 26-27 20.695589078285124 24.581159356587527 31.203718463195294 23.51953310193206 28-29 20.988133144965204 25.439855993178707 30.096982735831197 23.475028126024892 30-31 20.888298212859517 25.266211616165236 30.56442871848451 23.28106145249074 32-33 21.442427907137553 25.96683750551017 29.506431092592607 23.08430349475967 34-35 22.339023532363058 26.29115679753362 28.818475988039125 22.551343682064196 36-37 23.09771132770941 26.40996118750004 28.48039153692631 22.01193594786424 38-39 24.157187296277684 27.409388030741106 27.02865188554061 21.404772787440603 40-41 24.71472763757874 27.309565148162218 26.78778343849279 21.187923775766254 42-43 25.891400984035624 27.430444606279597 25.83973204135523 20.83842236832955 44-45 26.46046616190581 27.427096194351027 25.36287535940362 20.749562284339543 46-47 27.28163979005537 27.204728988077942 25.137439051659072 20.37619217020762 48-49 28.60130066914116 26.49010640303569 24.459701759681877 20.44889116814127 50-51 29.52165915433229 26.371844882577992 24.655744387907504 19.450751575182217 52-53 30.52940187915403 26.41210663532191 24.153717800076638 18.90477368544743 54-55 31.84238127104187 26.669628924721287 23.279209832110006 18.20877997212683 56-57 32.645285381628106 27.53460359415657 22.77967402414508 17.040437000070245 58-59 33.691557441624575 27.305675854659338 22.643829457221667 16.358937246494413 60-61 34.39260324093964 28.155325586873637 21.925030587886152 15.52704058430057 62-63 34.53394451066341 28.80798257183076 22.056094163286055 14.601978754219777 64-65 34.80625212846561 28.73929707015113 22.397713441640548 14.056737359742716 66-67 35.02177708093677 28.983327302921175 22.034188828030977 13.960706788111073 68-69 35.1190285069873 28.26314195069487 22.81605720562302 13.801772336694809 70-71 35.6301645246711 27.927852071639613 22.903842707566266 13.53814069612302 72-73 35.90577750525039 27.30405966520102 23.057293415190554 13.732869414358037 74-75 36.04735274654545 26.045032624880676 23.493291776949192 14.414322851624675 76-77 36.56502783228 24.821652196044248 23.458564682393924 15.154755289281832 78-79 36.62470106564381 23.720059442710255 23.93595419754343 15.719285294102503 80-81 36.704002082281065 22.848146208999324 24.03460081877253 16.413250889947072 82-83 36.05056068094063 22.401850288980324 24.433968043865526 17.113620986213522 84-85 35.149093263742486 22.20655853664986 24.992295567060527 17.65205263254712 86-87 34.24658911558795 22.484952165687115 25.138252298518076 18.13020642020685 88-89 32.815772257394954 23.87233423018551 25.53154398901115 17.780349523408383 90-91 31.409413606545193 25.61346675077426 25.402254925350785 17.574864717329756 92-93 29.810913991250743 27.711697587543995 25.28803323337978 17.189355187825488 94-95 28.165785898331404 30.393893021670287 24.73842427593083 16.70189680406748 96-97 26.700388826767696 33.07583809411633 23.952432921962643 16.271340157153332 98-99 25.130106087734987 36.08549718341745 23.04783965527571 15.73655707357185 100-101 23.704897829557307 38.91249287526628 22.249728965992702 15.132880329183706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1477.0 1 1043.0 2 538.0 3 524.0 4 592.0 5 759.0 6 1012.5 7 1230.0 8 1455.0 9 1731.0 10 2053.5 11 2379.0 12 2815.0 13 3356.5 14 4049.0 15 4966.5 16 6083.5 17 7630.0 18 10228.0 19 12943.0 20 15910.0 21 19824.0 22 24758.0 23 30286.0 24 37387.5 25 48156.5 26 60381.5 27 76468.5 28 97715.5 29 127539.0 30 170741.5 31 228055.5 32 319145.5 33 415575.0 34 499283.5 35 651288.0 36 862983.0 37 1123786.5 38 1525058.0 39 1818462.5 40 2117280.5 41 2765729.0 42 3610695.5 43 4591999.5 44 5675098.5 45 6707599.5 46 7612072.5 47 8326624.5 48 8632154.0 49 8401595.0 50 7843184.5 51 7114540.0 52 6271062.0 53 5400445.5 54 4502308.0 55 3644206.5 56 2860912.5 57 2170071.5 58 1617130.5 59 1180307.0 60 844575.0 61 597399.5 62 409489.0 63 267077.5 64 171505.5 65 109544.0 66 69645.0 67 44828.0 68 29658.0 69 20029.0 70 14079.5 71 9747.0 72 6778.0 73 4842.0 74 3658.0 75 2789.0 76 2040.5 77 1550.5 78 1153.5 79 825.0 80 619.5 81 499.5 82 411.5 83 337.5 84 267.0 85 216.0 86 198.0 87 172.5 88 146.5 89 139.0 90 119.5 91 94.5 92 94.0 93 104.5 94 114.5 95 109.0 96 86.0 97 65.0 98 33.0 99 21.5 100 39.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040937055639098635 2 0.03567333684742501 3 0.01871842399986907 4 0.004448552967593753 5 0.024891159944052808 6 0.024102808785238728 7 0.004806081837804222 8 0.002050428070657036 9 0.01034599168171543 10-11 0.0077588233946549266 12-13 0.0028714037388778246 14-15 0.005233328837705731 16-17 0.039823800119480786 18-19 0.02983310275253701 20-21 0.031050041644515892 22-23 0.0031386565693601494 24-25 0.0027324143905835046 26-27 0.03255389745483868 28-29 0.04611809587953608 30-31 0.0380996171428908 32-33 0.05437120293725698 34-35 0.047917359918870264 36-37 0.06371432613803205 38-39 0.09357245591148379 40-41 0.10193505618570665 42-43 0.09555316585244979 44-45 0.08749848731769569 46-47 0.07855624336264411 48-49 0.07432980520566862 50-51 0.0019476385204715268 52-53 0.028024453580359803 54-55 0.023544616836622633 56-57 0.007863400589191489 58-59 0.005736997633614728 60-61 0.0016647438019174936 62-63 0.0051023838889911475 64-65 0.0011096802309157412 66-67 0.0027587821447615265 68-69 0.0067546141804512745 70-71 0.03473348282985924 72-73 0.1468643685717929 74-75 0.037781416448403486 76-77 0.006014976330203368 78-79 0.09586734434714723 80-81 0.18701798907404288 82-83 0.0549848118607557 84-85 0.0510283080007891 86-87 0.07775671942663595 88-89 0.04019071412253428 90-91 0.01643694289683852 92-93 0.01828492024473888 94-95 0.021529494741898882 96-97 0.020872535442887145 98-99 0.019486664159733817 100-101 0.019900950738090197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.11879077E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.469177725418156 #Duplication Level Percentage of deduplicated Percentage of total 1 49.44479042749238 7.64870250718934 2 15.580879301168164 4.820467820561187 3 10.440249875582733 4.845062424694923 4 6.328885239265851 3.9161060227991618 5 3.889328091871451 3.0082353742810475 6 2.5528967099546938 2.3694727752554665 7 1.7161704211109434 1.858342167588963 8 1.2461958578281118 1.54221001643384 9 0.9128016016406957 1.270826118344364 >10 6.370085599514969 19.494855576117544 >50 0.7883601506711801 8.524184200001587 >100 0.6314842486538017 19.330053045678614 >500 0.0626264498563742 6.616407804926533 >1k 0.0325057891066437 9.19721934076353 >5k 0.0017968442792519223 1.9177654865085492 >10k+ 9.433920026745927E-4 3.6400893188548835 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 453787 0.40560488356549457 No Hit CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA 141331 0.12632478188928925 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 118810 0.10619501267426439 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 117687 0.10519125037114847 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 112175 0.10026450253964823 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.7540531372099184E-5 0.0 0.0 0.0 0.0 2 8.491310667498625E-5 2.681466526578513E-6 0.0 8.93822175526171E-7 0.0 3 1.9842852296680997E-4 2.681466526578513E-6 0.0 8.93822175526171E-7 0.0 4 2.922798513970579E-4 2.681466526578513E-6 0.0 1.787644351052342E-6 0.0 5 3.932817572315152E-4 2.681466526578513E-6 0.0 1.787644351052342E-6 0.0 6 4.585307760449257E-4 2.681466526578513E-6 0.0 2.681466526578513E-6 0.0 7 5.193106839807053E-4 2.681466526578513E-6 0.0 8.044399579735539E-6 0.0 8 6.256755228683197E-4 3.575288702104684E-6 0.0 8.044399579735539E-6 0.0 9 7.409785835111957E-4 3.575288702104684E-6 0.0 8.044399579735539E-6 8.93822175526171E-7 10-11 0.003662436364218486 3.575288702104684E-6 0.0 8.491310667498624E-6 8.93822175526171E-7 12-13 0.00417146809318064 5.809844140920111E-6 0.0 8.938221755261709E-6 8.93822175526171E-7 14-15 0.005441142493515566 8.044399579735539E-6 0.0 8.938221755261709E-6 8.93822175526171E-7 16-17 0.006930250237942167 1.0725866106314052E-5 0.0 8.938221755261709E-6 1.787644351052342E-6 18-19 0.008569967018944927 1.0725866106314052E-5 0.0 1.8323354598286505E-5 1.787644351052342E-6 20-21 0.0818070746150328 1.0725866106314052E-5 0.0 2.9496131792363644E-5 1.787644351052342E-6 22-23 0.11928414461266962 1.1619688281840222E-5 0.0 3.619979810880993E-5 1.787644351052342E-6 24-25 0.2167080802784957 1.2513510457366394E-5 0.0 3.888126463538844E-5 1.787644351052342E-6 26-27 0.5987558334969103 1.4301154808418737E-5 0.0 5.318241944380718E-5 1.787644351052342E-6 28-29 1.614563284250191 1.6088799159471077E-5 0.0 6.658975207669974E-5 1.787644351052342E-6 30-31 3.2518166913372015 1.6088799159471077E-5 0.0 7.150577404209367E-5 1.787644351052342E-6 32-33 5.663855271169246 1.6088799159471077E-5 0.0 7.642179600748762E-5 1.787644351052342E-6 34-35 8.591065691398223 1.6088799159471077E-5 0.0 8.401928449946008E-5 1.787644351052342E-6 36-37 12.25271236372463 1.6088799159471077E-5 0.0 8.759457320156475E-5 1.787644351052342E-6 38-39 16.335317996947722 1.6088799159471077E-5 0.0 8.982912864038018E-5 2.2345554388154277E-6 40-41 20.618531291601556 1.8770265686049592E-5 0.0 9.385132843024796E-5 2.681466526578513E-6 42-43 24.9652953429353 2.0110998949338846E-5 0.0 1.0591792779985127E-4 2.681466526578513E-6 44-45 29.27998637314464 2.1451732212628103E-5 0.0 1.1977217152050691E-4 2.681466526578513E-6 46-47 33.57245430260387 2.1451732212628103E-5 0.0 1.2960421545129479E-4 4.022199789867769E-6 48-49 37.73478485168411 2.1451732212628103E-5 8.93822175526171E-7 1.2960421545129479E-4 5.362933053157026E-6 50-51 41.76072260588993 2.1451732212628103E-5 8.93822175526171E-7 1.3317950415339948E-4 6.256755228683197E-6 52-53 45.54486179752806 2.1451732212628103E-5 8.93822175526171E-7 1.3317950415339948E-4 6.256755228683197E-6 54-55 49.139265333767455 2.1451732212628103E-5 8.93822175526171E-7 1.3452023741668875E-4 6.256755228683197E-6 56-57 52.54465676365922 2.1451732212628103E-5 8.93822175526171E-7 1.385424372065565E-4 6.256755228683197E-6 58-59 55.72486086920435 2.1451732212628103E-5 8.93822175526171E-7 1.416708148208981E-4 6.256755228683197E-6 60-61 58.74423016557421 2.3239376563680447E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 62-63 61.59079905530504 2.592084309025896E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 64-65 64.21854150620138 3.08368650556529E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 66-67 66.66360055866389 3.441215375775758E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 68-69 68.96725470840272 3.9328175723151525E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 70-71 71.06232338688315 4.1562731161966956E-5 8.93822175526171E-7 1.4569301461076587E-4 6.256755228683197E-6 72-73 73.01199267133747 4.647875312736089E-5 8.93822175526171E-7 1.4658683678629204E-4 6.256755228683197E-6 74-75 74.8333975797816 5.3182419443807175E-5 8.93822175526171E-7 1.4658683678629204E-4 6.256755228683197E-6 76-77 76.49344345234454 5.8098441409201113E-5 8.93822175526171E-7 1.4658683678629204E-4 6.256755228683197E-6 78-79 78.0517209665575 6.390828555012123E-5 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 80-81 79.47925240748992 7.195268512985676E-5 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 82-83 80.795603095653 8.178472906064465E-5 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 84-85 82.00783735461098 9.161677299143253E-5 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 86-87 83.09325254801664 9.87673503956419E-5 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 88-89 84.08974092626809 1.1172777194077136E-4 8.93822175526171E-7 1.4926830331287057E-4 6.256755228683197E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCATT 31645 0.0 66.83006 1 GGCATTC 49290 0.0 42.146824 2 CACGCGT 23325 0.0 38.930546 1 ACGCGTC 23885 0.0 38.9165 2 CGCGTGT 124125 0.0 34.546547 1 GTCGGAC 7110 0.0 33.26473 1 TGAACCG 24110 0.0 33.15332 12-13 GCGTGTT 134185 0.0 31.964693 2 CGGGGCT 16250 0.0 30.950443 1 GCATTCC 77820 0.0 30.432564 3 CGCGTCT 40715 0.0 29.543941 3 GCGGATT 14800 0.0 29.490221 1 ATTCCTG 72535 0.0 29.206835 5 CGGGTGT 15200 0.0 28.901632 1 GAACCGA 14865 0.0 27.389593 14-15 GGCGGAT 14940 0.0 26.861506 1 GAACCGC 15905 0.0 26.5987 14-15 CGGAGAA 32605 0.0 25.989649 2 ACCGCTC 11290 0.0 25.65401 16-17 GCGGGCT 9450 0.0 25.02775 1 >>END_MODULE