FastQCFastQC Report
Fri 27 May 2016
SRR1233077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111879077
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT3903570.3489097429718695No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG1453820.1299456555223458Illumina Paired End PCR Primer 2 (96% over 33bp)
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1434690.12823577370056424No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA1399720.1251100775527492No Hit
GGAAAACTTAAGAGGTACAACATGGAGAAGACATGATCACAGAATGGAAA1302660.1164346395170922No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1290010.1153039544650516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCT158800.032.1891331
GTTTATT755900.031.6057851
GGCGAAT117950.030.8571221
GCGAATT118850.029.4881462
ACTTAAG591900.029.2116476
GCGGATT151600.029.1300051
CGGATTT135800.028.3253652
GGCGGAT141200.027.699281
ATCGTCC45900.026.2787516
CGGCGGT32450.025.1039141
TAAGAGG644750.024.9464239
CCGGGCT120000.024.7721331
CGGGTGT107550.024.5391431
GTCGGGT105850.024.303171
CGGCCGT43950.023.8464551
GCGCGTC33950.023.779322
CGGGCAT155050.023.6887761
TCGGGTA82350.023.5857662
CGAATTT146850.023.2832183
GTCGGAC46950.023.1344551
Produced by FastQC (version 0.11.5)