##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111879077 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.25887154038641 39.0 37.0 39.0 34.0 39.0 2 36.966262467467445 39.0 37.0 39.0 33.0 39.0 3 36.95357457230363 39.0 37.0 39.0 33.0 39.0 4 36.91460470307598 39.0 37.0 39.0 33.0 39.0 5 36.88891858662724 39.0 37.0 39.0 33.0 39.0 6 36.94060229867645 39.0 37.0 39.0 33.0 39.0 7 36.89069107175419 39.0 37.0 39.0 33.0 39.0 8 36.860424500999414 39.0 37.0 39.0 33.0 39.0 9 36.83262681010498 39.0 36.0 39.0 33.0 39.0 10-11 36.824455501183664 38.5 36.5 39.0 33.0 39.0 12-13 36.727262064380454 38.0 36.0 39.0 33.0 39.0 14-15 36.62800854175799 38.0 36.0 39.0 32.5 39.0 16-17 36.560015493334824 38.0 36.0 39.0 32.5 39.0 18-19 36.48035710913132 38.0 36.0 39.0 32.0 39.0 20-21 36.43395204717322 38.0 36.0 39.0 32.0 39.0 22-23 36.30972233530314 38.0 36.0 39.0 31.0 39.0 24-25 36.17457264596489 38.0 36.0 39.0 31.0 39.0 26-27 35.95011988702767 38.0 36.0 39.0 31.0 39.0 28-29 35.74382479934117 38.0 35.0 39.0 30.0 39.0 30-31 35.55509068509745 38.0 35.0 39.0 30.0 39.0 32-33 35.33856552105806 37.5 35.0 39.0 29.0 39.0 34-35 35.16515530870888 37.0 35.0 39.0 29.0 39.0 36-37 34.92543541005438 37.0 34.5 39.0 28.0 39.0 38-39 34.95905418490358 37.0 35.0 39.0 28.0 39.0 40-41 34.99222940496729 37.0 34.5 39.0 28.5 39.0 42-43 34.99983254241542 37.0 35.0 39.0 28.5 39.0 44-45 34.811053634273364 37.0 34.5 39.0 28.0 39.0 46-47 34.70572224599243 37.0 34.5 39.0 28.0 39.0 48-49 34.5153670645674 37.0 34.0 39.0 27.5 39.0 50-51 34.286307139448425 37.0 34.0 39.0 27.0 39.0 52-53 33.996766173714505 37.0 33.5 39.0 26.0 39.0 54-55 33.73672866464567 37.0 33.0 39.0 25.5 39.0 56-57 33.37962673306645 36.5 33.0 39.0 24.5 39.0 58-59 33.0564364148267 36.0 32.5 38.5 24.0 39.0 60-61 32.74791610499254 36.0 32.0 38.0 23.0 39.0 62-63 32.31817651659747 36.0 32.0 37.0 21.5 39.0 64-65 31.894517426167184 36.0 31.0 37.0 20.0 39.0 66-67 31.276049788111855 35.0 30.5 37.0 16.0 39.0 68-69 30.634430444040937 35.0 29.5 37.0 11.5 39.0 70-71 29.807222202950424 34.5 28.5 37.0 2.0 39.0 72-73 28.906476279742634 34.0 27.0 36.0 2.0 38.0 74-75 28.288330846705144 33.0 25.5 36.0 2.0 37.0 76-77 28.84329769720928 34.5 27.5 37.0 2.0 39.0 78-79 28.083798523829437 34.0 25.5 37.0 2.0 39.0 80-81 27.37770674493498 34.0 23.5 37.0 2.0 39.0 82-83 26.502360132985366 33.0 20.5 37.0 2.0 39.0 84-85 25.51241556095426 32.5 12.5 36.0 2.0 38.5 86-87 23.70514916296637 31.0 2.0 36.0 2.0 37.5 88-89 22.635112604656186 30.0 2.0 35.0 2.0 37.0 90-91 20.9657805185504 28.0 2.0 35.0 2.0 37.0 92-93 19.422424029293698 25.5 2.0 34.0 2.0 37.0 94-95 18.213216730416896 22.0 2.0 33.5 2.0 36.5 96-97 16.413646834072466 6.5 2.0 32.5 2.0 36.0 98-99 15.440420629319279 2.0 2.0 31.5 2.0 36.0 100-101 14.657843458969545 2.0 2.0 31.0 2.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.17810435509925782 1 2 0.3172078002315999 1 3 0.3263349751670219 1 4 0.3425534003734825 1 5 0.3673533295115874 1 6 0.3828140725596185 1 7 0.3838993328858109 1 8 0.3834085875381916 1 9 0.4164818862931199 1 10-11 0.41840657850960383 1 12-13 0.45047671362785735 1 14-15 0.20491215036807375 1 16-17 0.32873122820705447 1 18-19 0.36358711831153556 1 20-21 0.3244357805232738 1 22-23 0.44778499328918997 1 24-25 0.46602299107747314 1 26-27 0.6100514729538133 1 28-29 0.5020653738902823 1 30-31 0.5388497491441271 1 32-33 0.4674345550065766 1 34-35 0.6565256619492743 1 36-37 0.41783219615217604 1 38-39 0.4903194224603311 1 40-41 0.32645008232513817 1 42-43 0.22563950636038754 1 44-45 0.005665676570004052 1 46-47 0.42044275931424124 1 48-49 0.49045263782916493 1 50-51 0.4452686888065571 1 52-53 0.7346787833948341 1 54-55 0.707160033336379 1 56-57 0.810508765549514 1 58-59 0.7907574050238466 1 60-61 0.8134100315375719 1 62-63 0.6705194563690284 1 64-65 0.45425485962564593 1 66-67 0.42061575414349406 1 68-69 0.3889207031465425 1 70-71 0.30113725896730514 1 72-73 0.2676843780491147 1 74-75 0.28056659526113137 1 76-77 0.21175930428333345 1 78-79 0.20831659835219085 1 80-81 0.03921652358745931 1 82-83 -0.1395567595531766 1 84-85 -0.3028073156311528 1 86-87 -0.6500100127594983 1 88-89 -0.8394023787329772 1 90-91 -1.1565477676160967 1 92-93 -1.38455146038741 1 94-95 -1.4282168642306274 1 96-97 -1.6417244865034384 1 98-99 -1.5977575592950686 1 100-101 -1.5261239371962443 2 1 0.11931047864509736 2 2 0.1919984545446809 2 3 0.21604617584329588 2 4 0.16988765570119568 2 5 0.22630918147671508 2 6 0.2199115385104733 2 7 0.20844200457418793 2 8 0.2412851821737121 2 9 0.13350188653806327 2 10-11 0.24468686288496144 2 12-13 0.290909512747767 2 14-15 0.32155057403468135 2 16-17 0.3122687447171657 2 18-19 0.285457970331656 2 20-21 0.31963499979435994 2 22-23 0.311335094108216 2 24-25 0.34580391371896013 2 26-27 0.3928013639499923 2 28-29 0.4551800743467851 2 30-31 0.5093575855490684 2 32-33 0.557093745404643 2 34-35 0.5815414970310471 2 36-37 0.5803555346159612 2 38-39 0.5900213935825036 2 40-41 0.5610982389422503 2 42-43 0.5318556224864324 2 44-45 0.5384304407244542 2 46-47 0.4730087427207721 2 48-49 0.4394890622809555 2 50-51 0.4690712072277208 2 52-53 0.4232596224809839 2 54-55 0.45291434367280203 2 56-57 0.44297970467279413 2 58-59 0.5017299678614293 2 60-61 0.4526485106670748 2 62-63 0.42512979026933095 2 64-65 0.3901842555195749 2 66-67 0.33230583669510594 2 68-69 0.41434423281498667 2 70-71 0.34301001623274985 2 72-73 0.2863270900656083 2 74-75 0.1635736802609351 2 76-77 0.04256207508840504 2 78-79 -0.05098745685235784 2 80-81 -0.3134045077775305 2 82-83 -0.3183722679289609 2 84-85 -0.328069115587617 2 86-87 -0.44434968952501563 2 88-89 -0.5938342145604949 2 90-91 -0.7318241645736308 2 92-93 -0.8433651869089687 2 94-95 -0.8426579406025851 2 96-97 -0.9184050363536116 2 98-99 -0.9059773565446729 2 100-101 -0.863883332501862 3 1 0.053949803113567896 3 2 0.10224570608686889 3 3 0.12010697069741383 3 4 0.12581293500748103 3 5 0.11768273426783082 3 6 0.16720131206373878 3 7 0.16192345720096313 3 8 0.16155159531590613 3 9 0.17031097025705577 3 10-11 0.19430313928958753 3 12-13 0.23360547230252138 3 14-15 0.25449018337410934 3 16-17 0.3168965104331889 3 18-19 0.3194590842661782 3 20-21 0.26613480475607076 3 22-23 0.21505585041259678 3 24-25 0.23840134966163617 3 26-27 0.23356017913736338 3 28-29 0.2976147279660353 3 30-31 0.3309294766816393 3 32-33 0.34835617197484225 3 34-35 0.27098219916972255 3 36-37 0.3451177470227691 3 38-39 0.3846312640894425 3 40-41 0.361548457479536 3 42-43 0.35367330003480646 3 44-45 0.35889187975797654 3 46-47 0.3217378555275303 3 48-49 0.29352851400974345 3 50-51 0.25526216272158564 3 52-53 0.26312874128949915 3 54-55 0.1700094361988107 3 56-57 0.23309231315527512 3 58-59 0.1566950045442752 3 60-61 0.2963682363388145 3 62-63 0.23257813879754252 3 64-65 0.26457917811820764 3 66-67 0.22917627823816034 3 68-69 0.14736233153850264 3 70-71 0.22196974637740752 3 72-73 0.23211457713366812 3 74-75 0.12516309263351033 3 76-77 0.12661573731463704 3 78-79 0.1916342601861416 3 80-81 0.10678295641364244 3 82-83 0.06556599711676014 3 84-85 0.12300182310629282 3 86-87 0.07885457951518049 3 88-89 0.12605143173168898 3 90-91 0.09869373933257108 3 92-93 -0.02040869523951727 3 94-95 0.011897528646976241 3 96-97 -0.1280412175918535 3 98-99 -0.16651868711464246 3 100-101 -0.0675588452643634 4 1 -0.029918957004291258 4 2 -0.038110970065687866 4 3 -0.023351711748574644 4 4 -0.0033252758974455787 4 5 -0.004887595012881718 4 6 0.042331749837771326 4 7 0.052993879531591404 4 8 -0.013476444424227907 4 9 0.04850845971329676 4 10-11 0.08429997802166156 4 12-13 0.09784898626191563 4 14-15 0.1430379487548592 4 16-17 0.16650755790229965 4 18-19 0.14872939773314897 4 20-21 0.07266052207689455 4 22-23 0.06701449310998697 4 24-25 0.037984612113383776 4 26-27 0.02853209485459729 4 28-29 0.0278964625030369 4 30-31 0.013295266988286869 4 32-33 0.016237517823114445 4 34-35 0.05574277775752989 4 36-37 0.06504142652950407 4 38-39 0.03482817523664039 4 40-41 0.07445040163191408 4 42-43 0.10176130875246514 4 44-45 0.0032488497637075398 4 46-47 -0.027017101792765175 4 48-49 0.06006546168499227 4 50-51 0.07508142251023742 4 52-53 0.12534683407248792 4 54-55 0.0803590742267204 4 56-57 0.04387698792440631 4 58-59 0.06856330788679088 4 60-61 0.05681462916342639 4 62-63 0.03750586043030779 4 64-65 0.0860709328759448 4 66-67 -0.07080345116714071 4 68-69 -0.0334540514199233 4 70-71 -0.011419768345678705 4 72-73 -0.11888943062687929 4 74-75 0.004652747525550893 4 76-77 0.13320149804225068 4 78-79 0.2610137734242173 4 80-81 0.3746131588978976 4 82-83 0.46245449985248754 4 84-85 0.4829020300810285 4 86-87 0.4804534667369147 4 88-89 0.6249551357866601 4 90-91 0.6553078534720349 4 92-93 0.6833545955903801 4 94-95 0.7581482052087409 4 96-97 0.7562940009400982 4 98-99 0.7050421412943528 4 100-101 0.7590654645171906 5 1 -0.062086277626356434 5 2 -0.06265845732452391 5 3 -0.05083401960249034 5 4 -0.06481735926478649 5 5 -0.054994402699691136 5 6 -0.025168237179258313 5 7 -0.036952184786471776 5 8 -0.021226594168723523 5 9 -0.042494835715125134 5 10-11 -0.04340449288515913 5 12-13 0.004344081079793227 5 14-15 0.025700505140711982 5 16-17 -0.03817669718393546 5 18-19 0.019609133438393656 5 20-21 -0.03330574197458702 5 22-23 -0.04017750367107453 5 24-25 -0.06562854969038767 5 26-27 -0.07205670358695926 5 28-29 -0.10154997838546365 5 30-31 -0.40938389543519094 5 32-33 -0.5211117796446203 5 34-35 -0.3121613824262326 5 36-37 -0.604342432726277 5 38-39 -0.39259830679898755 5 40-41 -0.16720590994152218 5 42-43 -0.08801078401649676 5 44-45 -0.23376048888290768 5 46-47 -0.2891554310099522 5 48-49 -0.22480214662252251 5 50-51 -0.15507602968003908 5 52-53 -0.19523851087588184 5 54-55 -0.14171544116693724 5 56-57 -0.2405296664239529 5 58-59 -0.17088884218085099 5 60-61 -0.09170972595615012 5 62-63 -0.0982596292416602 5 64-65 -0.04813445032574393 5 66-67 -0.13326305935136062 5 68-69 -0.1521820857377385 5 70-71 -0.03633367888811634 5 72-73 -0.06825870845689153 5 74-75 0.02719914158189951 5 76-77 -0.00320595963820125 5 78-79 0.10183374066915007 5 80-81 0.21892318284037415 5 82-83 0.2903881706971454 5 84-85 0.49598153901170505 5 86-87 0.8695590009306251 5 88-89 0.9493681966104255 5 90-91 1.0882922896708607 5 92-93 1.1618108058644623 5 94-95 1.2107767890337016 5 96-97 1.199676418161502 5 98-99 1.075925092497286 5 100-101 1.0733846964554612 6 1 -0.20027299862223202 6 2 -0.22608567485848852 6 3 -0.2264764542062423 6 4 -0.21645520314033462 6 5 -0.228460784114624 6 6 -0.2147104980175314 6 7 -0.1979448184483985 6 8 -0.20027386219268095 6 9 -0.20064237414207042 6 10-11 -0.2179227049071173 6 12-13 -0.2701375181938843 6 14-15 -0.200351449949828 6 16-17 -0.20419709067274994 6 18-19 -0.1922105164710075 6 20-21 -0.2630360135464258 6 22-23 -0.24612841468578495 6 24-25 -0.25122845916045833 6 26-27 -0.32628289031395497 6 28-29 -0.28909080252604014 6 30-31 -0.24182493678281958 6 32-33 -0.2831469826982129 6 34-35 -0.3306879128940423 6 36-37 -0.2768336440814281 6 38-39 -0.2758630714057375 6 40-41 -0.20963773731813973 6 42-43 -0.18538665605007765 6 44-45 -0.17755190438511192 6 46-47 -0.2659785848718528 6 48-49 -0.2783982510904508 6 50-51 -0.28226244642741705 6 52-53 -0.20348300861013513 6 54-55 -0.24839380796591115 6 56-57 -0.24689215839073597 6 58-59 -0.37379348546755153 6 60-61 -0.37356807139137516 6 62-63 -0.26260001785415454 6 64-65 -0.2991712775476252 6 66-67 -0.2624680526925012 6 68-69 -0.19739883981280215 6 70-71 -0.1756846925566542 6 72-73 -0.11400339138116422 6 74-75 -0.10561685037561475 6 76-77 0.19822074531081668 6 78-79 0.37719074577028877 6 80-81 0.5957677395046233 6 82-83 0.7875052076766487 6 84-85 0.9435510795325293 6 86-87 1.2450203218197267 6 88-89 1.3873812624395967 6 90-91 1.7104986846956258 6 92-93 1.7952359778084634 6 94-95 1.8865030512614709 6 96-97 2.022220229710971 6 98-99 1.931223214975482 6 100-101 1.8627641374284014 7 1 -0.3265630005518432 7 2 -0.36742179216895465 7 3 -0.3451007256592007 7 4 -0.3602873200444705 7 5 -0.37355964582214085 7 6 -0.3876089791525743 7 7 -0.3953799455302658 7 8 -0.38022983223387286 7 9 -0.38506020421069564 7 10-11 -0.41364689164106494 7 12-13 -0.4145810806926491 7 14-15 -0.39091661088089324 7 16-17 -0.3973191392933231 7 18-19 -0.36684755790400914 7 20-21 -0.3825029060099183 7 22-23 -0.35475163635729245 7 24-25 -0.39626262929217404 7 26-27 -0.4170824929346253 7 28-29 -0.46688833525149676 7 30-31 -0.4316076089876688 7 32-33 -0.4655309713025133 7 34-35 -0.43077911047697626 7 36-37 -0.41740732255411217 7 38-39 -0.464722971089536 7 40-41 -0.3967180857627639 7 42-43 -0.40503441720367306 7 44-45 -0.35385386302760935 7 46-47 -0.2869327535952877 7 48-49 -0.3455960463100567 7 50-51 -0.2860110380145997 7 52-53 -0.23674898979847114 7 54-55 -0.22802718077800677 7 56-57 -0.29884244783508507 7 58-59 -0.4023589784057364 7 60-61 -0.33311802543573776 7 62-63 -0.34226054271641004 7 64-65 -0.20445085371723692 7 66-67 -0.1976834633613933 7 68-69 -0.11148368690474086 7 70-71 0.12447343006807188 7 72-73 0.19377319350942557 7 74-75 0.2427545284816155 7 76-77 0.4629332985599497 7 78-79 0.7601501835612865 7 80-81 1.0327847166103616 7 82-83 1.2697505493731 7 84-85 1.4373293440156552 7 86-87 1.951363922767488 7 88-89 2.1173199081077954 7 90-91 2.413182275424461 7 92-93 2.54687741942476 7 94-95 2.6204975384411178 7 96-97 2.873318240835456 7 98-99 2.794132932923226 7 100-101 2.716898961147054 8 1 -0.690195285166638 8 2 -0.6877059267319865 8 3 -0.7540210307508843 8 4 -0.7740549716432525 8 5 -0.6222145205737135 8 6 -0.7816796276299343 8 7 -0.7481981392997739 8 8 -0.7829570461378452 8 9 -0.714210016255798 8 10-11 -0.9462808326080108 8 12-13 -0.806434473772832 8 14-15 -0.8037514163922381 8 16-17 -0.8362921178602178 8 18-19 -0.7923513810257958 8 20-21 -0.8134818998057227 8 22-23 -0.8852603025429815 8 24-25 -0.8334146031639875 8 26-27 -0.9170230789269738 8 28-29 -0.8792509984425152 8 30-31 -1.088090022809105 8 32-33 -1.2281862172774396 8 34-35 -1.1550249529059897 8 36-37 -1.0894462102750992 8 38-39 -0.9933502679289603 8 40-41 -1.0345123084468213 8 42-43 -1.415192354763441 8 44-45 -1.26674267249237 8 46-47 -0.8389556034124368 8 48-49 -1.0146659801605296 8 50-51 -1.1658680586673569 8 52-53 -0.993884229288561 8 54-55 -1.01947170610012 8 56-57 -0.8043039435584802 8 58-59 -0.8365891798999385 8 60-61 -0.7586729007751245 8 62-63 -0.7433213014499316 8 64-65 -0.685495347664979 8 66-67 -0.9579977705423737 8 68-69 -0.5224002223264606 8 70-71 -0.6885833413900961 8 72-73 -0.9511906071935918 8 74-75 -0.8848445992654099 8 76-77 -0.3395267444018941 8 78-79 -0.13358904480993417 8 80-81 -0.03547981646120135 8 82-83 0.6221208720757545 8 84-85 0.8846580986354837 8 86-87 2.0208157140382177 8 88-89 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-0.4253135275058284 65 46-47 -0.09116356394361702 65 48-49 -0.06519580244020062 65 50-51 -0.11681018791309583 65 52-53 -0.06366671245496036 65 54-55 -0.02006529838952531 65 56-57 0.060003239143725295 65 58-59 0.05580563407888661 65 60-61 -0.04770311771399349 65 62-63 0.04227469363718939 65 64-65 -0.05853432957718141 65 66-67 0.014046192023016602 65 68-69 0.03012202265486863 65 70-71 -0.03966328564501964 65 72-73 -0.08454805763777884 65 74-75 -0.080173982008791 65 76-77 -0.10273234910281204 65 78-79 -0.1411198708874224 65 80-81 -0.18042434227321635 65 82-83 -0.21540820713623177 65 84-85 -0.3270967792644299 65 86-87 -0.4805867045026808 65 88-89 -0.5602399731442596 65 90-91 -0.6873040380120585 65 92-93 -0.7146335737485785 65 94-95 -0.7691948370182686 65 96-97 -0.8211362329182279 65 98-99 -0.8287418676592857 65 100-101 -0.8361585176468296 66 1 0.0022661292839174507 66 2 -0.06949528660621951 66 3 -0.05127628720307342 66 4 -0.05784683472570151 66 5 -0.07560238948280329 66 6 -0.08911936640645024 66 7 -0.11340247424040228 66 8 -0.10387092893634531 66 9 -0.10446492337644031 66 10-11 -0.11375435350413454 66 12-13 -0.12043932683733516 66 14-15 -0.14773026839078085 66 16-17 -0.1056161020619939 66 18-19 -0.11774652394834106 66 20-21 -0.1131553599170303 66 22-23 -0.11506107049930137 66 24-25 -0.11747311001089145 66 26-27 -0.15640433840472667 66 28-29 -0.17422285968547158 66 30-31 -0.14677970530495799 66 32-33 -0.12676799554947138 66 34-35 -0.17417555691179132 66 36-37 -0.12299238008085212 66 38-39 -0.12305400439829839 66 40-41 -0.043700137021936314 66 42-43 -0.060705714515030706 66 44-45 -0.1296856200232952 66 46-47 -0.13330886105169526 66 48-49 -0.14017182868579425 66 50-51 -0.2530440681771893 66 52-53 -0.14870506600686895 66 54-55 -0.1717903814797168 66 56-57 -0.19620420244020664 66 58-59 -0.12135496090741782 66 60-61 -0.18068321205802107 66 62-63 -0.12225425662718692 66 64-65 -0.11335054803957334 66 66-67 -0.14906907830117788 66 68-69 -0.058593137574636245 66 70-71 -0.11218238338461006 66 72-73 -0.07756117833529785 66 74-75 -0.08808380737596266 66 76-77 0.0027321062187297684 66 78-79 0.0363969671843698 66 80-81 0.0506329267770802 66 82-83 0.14249824808099376 66 84-85 0.10520644678889823 66 86-87 0.07018507961180376 66 88-89 0.10412262367049152 66 90-91 0.17091393418895962 66 92-93 0.1255944380217855 66 94-95 0.09135564531472085 66 96-97 -0.05824385121863784 66 98-99 -0.09713703381386019 66 100-101 -0.14637432138993667 67 1 -0.1526025544848082 67 2 -0.2513968217152822 67 3 -0.23757063165899694 67 4 -0.2425248128742794 67 5 -0.3039085007612101 67 6 -0.3258327198717055 67 7 -0.29073636751216014 67 8 -0.26725222529737636 67 9 -0.2768654587810886 67 10-11 -0.2939277890042433 67 12-13 -0.34663015410994547 67 14-15 -0.38115079150968967 67 16-17 -0.33206253382595463 67 18-19 -0.32932062919960714 67 20-21 -0.3419292225372246 67 22-23 -0.32498497040300833 67 24-25 -0.346124117543674 67 26-27 -0.3822567700421473 67 28-29 -0.396513350740733 67 30-31 -0.3952772877007149 67 32-33 -0.37298153976357185 67 34-35 -0.3996361967752904 67 36-37 -0.37843833759808376 67 38-39 -0.4015441916585445 67 40-41 -0.36977964972245303 67 42-43 -0.36010056163908644 67 44-45 -0.32630411914938406 67 46-47 -0.41090210845850805 67 48-49 -0.3142358060664847 67 50-51 -0.33281519022271056 67 52-53 -0.23125661932675712 67 54-55 -0.31229375843068397 67 56-57 -0.2582910859765377 67 58-59 -0.35281944592659187 67 60-61 -0.27368336811463223 67 62-63 -0.2917811635077783 67 64-65 -0.22100173444900761 67 66-67 -0.19014344905411562 67 68-69 -0.11375027432434237 67 70-71 -0.10861193654578472 67 72-73 0.027160881498648592 67 74-75 0.0748253543268973 67 76-77 0.23963040170745842 67 78-79 0.343656124005598 67 80-81 0.5675295957155129 67 82-83 0.7413114805411034 67 84-85 0.8030246888620702 67 86-87 0.882413875075617 67 88-89 1.010254613212453 67 90-91 1.056696521345259 67 92-93 1.0540172477810827 67 94-95 1.0729885006138247 67 96-97 1.0133126181653687 67 98-99 0.9473728921787412 67 100-101 0.759481474294013 68 1 -0.45894919830624303 68 2 -0.5943831204099155 68 3 -0.5671169452221392 68 4 -0.653716573710696 68 5 -0.6500573362448776 68 6 -0.6484012281852713 68 7 -0.6260531794100928 68 8 -0.632608269691346 68 9 -0.6541947519858695 68 10-11 -0.6194505127358667 68 12-13 -0.6734596025256465 68 14-15 -0.688859291082828 68 16-17 -0.7659074351069393 68 18-19 -0.6948664064933965 68 20-21 -0.722652310560143 68 22-23 -0.7880273880059576 68 24-25 -0.7867917394788861 68 26-27 -0.8994635261066151 68 28-29 -0.8644926298141513 68 30-31 -0.8065128586328285 68 32-33 -0.7284248883998998 68 34-35 -0.7927095796036525 68 36-37 -0.8480401277213119 68 38-39 -0.7462671157848035 68 40-41 -0.8243930451247152 68 42-43 -0.8329120442633098 68 44-45 -0.7667832059752584 68 46-47 -0.9083211858096334 68 48-49 -0.9283820298802397 68 50-51 -0.8438970515232995 68 52-53 -0.7085313902356063 68 54-55 -0.8155466656249928 68 56-57 -0.9512148997658443 68 58-59 -0.9474310752828004 68 60-61 -0.7347455191136234 68 62-63 -0.7874549658751633 68 64-65 -0.9664819272616505 68 66-67 -0.36588146827665113 68 68-69 -0.4261125562832362 68 70-71 -0.22704738053039009 68 72-73 0.055412243745298184 68 74-75 0.025166691409875597 68 76-77 0.04435573889745825 68 78-79 -0.17545070246378458 68 80-81 0.18036266945184565 68 82-83 0.4157831958523879 68 84-85 0.5287920289924379 68 86-87 1.2327174075040723 68 88-89 1.5377187171319342 68 90-91 1.9702313867213057 68 92-93 2.3532666683280006 68 94-95 2.433237246574791 68 96-97 2.477913688676434 68 98-99 2.4161374023786024 68 100-101 2.1492815293061067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 218302.0 3 33062.0 4 60137.0 5 81605.0 6 133193.0 7 186910.0 8 249888.0 9 290545.0 10 337977.0 11 382655.0 12 469372.0 13 469397.0 14 573013.0 15 630728.0 16 736707.0 17 841727.0 18 970675.0 19 1134968.0 20 1316497.0 21 1552945.0 22 1809175.0 23 2129504.0 24 2433285.0 25 2753143.0 26 3092125.0 27 3548668.0 28 4195520.0 29 4957481.0 30 5711503.0 31 6388491.0 32 6987955.0 33 7518355.0 34 8427254.0 35 1.018206E7 36 1.2315386E7 37 1.2449254E7 38 6309600.0 39 15.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.295503251850214 17.24205583352247 10.858010749362444 33.60443016526487 2 22.906591412558885 25.576571329371234 32.84928613718389 18.667551120885996 3 27.12831486023356 28.041651863481604 20.777611713561168 24.052421562723666 4 28.64853031142302 33.62838910110412 19.88040194397029 17.84267864350256 5 25.374828496584172 33.706805676888315 24.507105969256937 16.41125985727058 6 21.277925391054666 38.57573300295513 22.542005652157346 17.60433595383286 7 18.69684892019622 21.147678935535016 40.0527785905849 20.10269355368386 8 22.82833366599905 27.032372639255865 28.04727196667881 22.09202172806628 9 21.914544396893803 27.01267994908467 28.613720150730238 22.45905550329129 10-11 24.785415975434873 35.79009163523083 19.905765847110832 19.51872654222347 12-13 22.82204101695495 29.294535509457265 26.707003402079415 21.176420071508367 14-15 23.397377396414562 30.55279534231182 25.643395349937027 20.40643191133659 16-17 23.842005380487365 30.956372885634014 24.56456746398875 20.63705426988987 18-19 23.725601079100787 31.695287403917355 24.255365907246446 20.32374560973541 20-21 23.98237965441921 31.179998025904343 24.068559307116917 20.76906301255953 22-23 23.594511409738452 31.477737425054674 23.97617877842532 20.95157238678155 24-25 23.58189260925796 30.95987569008146 24.57789648955167 20.880335211108907 26-27 23.285190301912735 31.138907377327456 24.34875446325448 21.22714785750533 28-29 23.38592096211593 31.808475655120887 23.67248762695448 21.133115755808703 30-31 23.368546218972696 31.473192484472285 24.115772014978525 21.04248928157649 32-33 24.018341604882828 32.07075743966287 22.952093479760304 20.95880747569399 34-35 24.725167709120118 32.08832211588475 22.748788548043652 20.43772162695148 36-37 25.361787721289836 32.14827301622192 22.268410594575045 20.221528667913198 38-39 26.2967175483713 32.87781112907012 21.226730790006215 19.598740532552366 40-41 26.91277643366724 32.526868499182264 20.997742697251205 19.56261236989929 42-43 28.279004683460663 32.32013946451468 20.28458958881545 19.1162662632092 44-45 28.646044840271646 32.1243611078027 20.068249205777086 19.16134484614857 46-47 29.388487893942848 31.850094723251964 19.792521169977118 18.96889621282807 48-49 29.93834235902527 31.428347900273266 19.09549858349236 19.537811157209095 50-51 30.703879666296825 31.253753010152508 19.036844085219347 19.005523238331325 52-53 31.11088093703167 31.056756595645723 18.98127078622422 18.851091681098385 54-55 31.749587921874827 30.778605465202997 18.31166091580784 19.160145697114338 56-57 31.89924617665944 31.083929497723496 17.802445285761056 19.21437903985601 58-59 32.55559673160693 30.386139391096446 17.661398615734665 19.39686526156196 60-61 32.72909078613127 30.675865686359703 16.649027960743982 19.94601556676504 62-63 32.66760441064706 30.568976966802587 16.590316961151025 20.173101661399333 64-65 32.79279881388363 30.256469879339136 16.24949509611099 20.70123621066624 66-67 32.552509856085514 30.295644442253096 15.523934575741594 21.627911125919795 68-69 32.435061360718095 29.824845977854164 15.88998624001611 21.850106421411628 70-71 31.921366583940554 29.74438833354407 16.186985411457403 22.147259671057974 72-73 31.684479426979493 29.522972729620605 16.437482738238764 22.355065105161138 74-75 31.153293691284095 28.81818464147449 17.18450651915363 22.844015148087777 76-77 30.559446970601766 28.289637101249802 17.783242383982934 23.3676735441655 78-79 30.06886567097459 27.117709398117036 18.89304543414497 23.92037949676341 80-81 29.40498344571521 26.262689018838458 20.10315609607352 24.229171439372813 82-83 28.80041257133633 25.12217429114518 21.272483001614205 24.804930135904286 84-85 28.193597223574823 23.76553916425188 22.571407338607273 25.46945627356602 86-87 27.474404903271843 22.648332560158444 24.15289125285229 25.724371283717428 88-89 26.663422767069594 21.973250931919473 25.556331194247374 25.80699510676355 90-91 25.65862296451764 21.905441341419806 26.766595847978223 25.669339846084327 92-93 24.807104802443273 22.63696983688747 27.515290365199014 25.04063499547024 94-95 23.631895319967146 23.923693324055908 27.891831364486343 24.552579991490607 96-97 22.541648965466678 25.595806693264777 27.95504944369461 23.907494897573937 98-99 21.363860317348173 28.122788702904167 27.549207090788368 22.964143888959292 100-101 19.73051984207198 31.121149376500618 27.65896593364244 21.489364847784962 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 363.0 1 200.0 2 33.5 3 30.0 4 39.0 5 48.0 6 57.0 7 81.5 8 120.5 9 173.5 10 233.0 11 317.5 12 474.5 13 689.5 14 913.0 15 1230.5 16 1720.0 17 2415.5 18 3468.0 19 5327.5 20 8554.0 21 11298.0 22 14249.5 23 19994.5 24 28154.5 25 37920.5 26 50046.0 27 67293.5 28 89809.5 29 122048.5 30 169513.0 31 223696.5 32 308209.5 33 401197.0 34 470923.0 35 594388.0 36 758524.0 37 968085.5 38 1275117.0 39 1450701.0 40 1633792.0 41 2034005.5 42 2519132.5 43 3070094.0 44 3847586.5 45 4867925.5 46 6006340.0 47 7078452.5 48 7949904.0 49 8535186.5 50 8662675.5 51 8406706.0 52 7859985.5 53 7053631.5 54 6045583.0 55 4983331.5 56 3959091.5 57 3026519.5 58 2246364.5 59 1619325.5 60 1135739.5 61 786345.0 62 537690.0 63 349171.5 64 214200.5 65 129447.0 66 78665.0 67 48805.0 68 31316.5 69 21127.0 70 14907.0 71 10337.0 72 7257.5 73 5277.5 74 3895.0 75 2889.5 76 2173.5 77 1683.0 78 1279.0 79 904.5 80 659.5 81 497.0 82 377.5 83 289.5 84 225.5 85 148.5 86 99.5 87 75.5 88 51.0 89 40.0 90 41.5 91 36.5 92 29.5 93 32.0 94 32.0 95 30.0 96 27.0 97 24.5 98 17.0 99 19.5 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31320959145917876 2 0.0012683336670716366 3 8.93822175526171E-7 4 8.93822175526171E-7 5 9.832043930787882E-5 6 2.3239376563680444E-5 7 0.0 8 0.0 9 0.0 10-11 4.469110877630855E-7 12-13 1.3407332632892565E-6 14-15 1.2558201566142702E-4 16-17 1.5954725833142153E-4 18-19 0.0 20-21 0.0 22-23 6.860085197163363E-4 24-25 0.007757929572479402 26-27 0.008594547128771897 28-29 0.0055019224014513455 30-31 0.0016535710247234164 32-33 8.893530646485401E-5 34-35 5.546166599139891E-4 36-37 0.0014238587256131903 38-39 4.6523444236137195E-4 40-41 9.693501493581323E-4 42-43 0.0012893384881965016 44-45 7.615364935482977E-4 46-47 0.0 48-49 0.00398599999175896 50-51 0.021330172396756546 52-53 0.04097772454808507 54-55 0.009316755446597044 56-57 0.02497071011767464 58-59 0.012415636929146278 60-61 0.00179747639498313 62-63 0.003429148776406155 64-65 0.004524974763601241 66-67 0.004284983509472463 68-69 0.00612536336888085 70-71 0.004728766219621208 72-73 0.0038143861340579352 74-75 0.0011847612936599396 76-77 2.887045626949532E-4 78-79 0.04585173687122928 80-81 0.056050694805070654 82-83 0.04094688768302942 84-85 0.05147253762202561 86-87 0.005309750633713218 88-89 0.0012379437131037469 90-91 0.0037379643380504473 92-93 0.0015020681659717303 94-95 0.0034961854395706175 96-97 0.0041111350963326234 98-99 0.002931736735725841 100-101 0.005211877105493103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.11879077E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.55144670976775 #Duplication Level Percentage of deduplicated Percentage of total 1 46.64892823407387 6.788093932659062 2 16.5486362164183 4.81613196045087 3 10.854537633786384 4.738476778116333 4 6.625615965340886 3.856491905561773 5 4.160162481242207 3.026819132488558 6 2.703429376706477 2.360328510525897 7 1.8001045905113329 1.8335888214583773 8 1.3206663426988376 1.5374084725732806 9 0.9617585495015754 1.2595480452662133 >10 6.734200840462394 19.454946561211763 >50 0.852086768040603 8.688673531468737 >100 0.6822916781684376 19.696820164428384 >500 0.06890317140909319 6.857209561821347 >1k 0.03552368182079871 9.342822700547817 >5k 0.0023297454099697494 2.3497644414794085 >10k+ 8.247244092282022E-4 3.392875479942355 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 390357 0.3489097429718695 No Hit CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG 145382 0.1299456555223458 Illumina Paired End PCR Primer 2 (96% over 33bp) GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 143469 0.12823577370056424 No Hit CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA 139972 0.1251100775527492 No Hit GGAAAACTTAAGAGGTACAACATGGAGAAGACATGATCACAGAATGGAAA 130266 0.1164346395170922 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 129001 0.1153039544650516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.664670919657301E-5 1.787644351052342E-6 0.0 0.0 0.0 2 8.31254623239339E-5 3.575288702104684E-6 0.0 0.0 0.0 3 2.0110998949338847E-4 3.575288702104684E-6 0.0 0.0 0.0 4 2.949613179236364E-4 3.575288702104684E-6 0.0 0.0 0.0 5 3.932817572315152E-4 3.575288702104684E-6 0.0 0.0 0.0 6 4.6031842039597805E-4 5.362933053157026E-6 0.0 8.93822175526171E-7 8.93822175526171E-7 7 5.202045061562315E-4 5.362933053157026E-6 0.0 2.681466526578513E-6 8.93822175526171E-7 8 6.238878785172673E-4 1.1619688281840224E-5 0.0 2.681466526578513E-6 8.93822175526171E-7 9 7.374032948090911E-4 1.1619688281840224E-5 0.0 2.681466526578513E-6 8.93822175526171E-7 10-11 0.0036624363642184855 1.4301154808418737E-5 0.0 2.681466526578513E-6 8.93822175526171E-7 12-13 0.004163870604688667 2.0557910037101933E-5 0.0 2.681466526578513E-6 8.93822175526171E-7 14-15 0.0054326511828480676 2.4133198739206618E-5 0.0 2.681466526578513E-6 8.93822175526171E-7 16-17 0.006923099660537957 2.4133198739206618E-5 0.0 4.469110877630855E-6 8.93822175526171E-7 18-19 0.008571307752208216 2.45801098269697E-5 0.0 1.0725866106314052E-5 8.93822175526171E-7 20-21 0.09121678756788457 2.5027020914732788E-5 0.0 1.3407332632892565E-5 8.93822175526171E-7 22-23 0.1291367464534946 2.5027020914732788E-5 0.0 1.5194976983944907E-5 8.93822175526171E-7 24-25 0.2271573084214844 2.5027020914732788E-5 0.0 2.0557910037101933E-5 8.93822175526171E-7 26-27 0.6107826577797026 2.5473932002495875E-5 0.0 2.5027020914732788E-5 8.93822175526171E-7 28-29 1.7314819284753304 2.592084309025896E-5 0.0 3.0389953967889814E-5 8.93822175526171E-7 30-31 3.4074936996485947 3.128377614341599E-5 0.0 3.485906484552067E-5 8.93822175526171E-7 32-33 5.856413616998289 3.307142049446833E-5 0.0 3.485906484552067E-5 8.93822175526171E-7 34-35 8.788136051569321 3.485906484552067E-5 0.0 3.9328175723151525E-5 8.93822175526171E-7 36-37 12.4523001740531 3.485906484552067E-5 2.681466526578513E-6 4.335037551301929E-5 8.93822175526171E-7 38-39 16.54159919463762 3.485906484552067E-5 2.681466526578513E-6 4.647875312736089E-5 8.93822175526171E-7 40-41 20.830737189581928 3.485906484552067E-5 2.681466526578513E-6 4.91602196539394E-5 8.93822175526171E-7 42-43 25.182790433639347 3.664670919657301E-5 2.681466526578513E-6 5.2735508356044084E-5 8.93822175526171E-7 44-45 29.503313653543998 3.664670919657301E-5 2.681466526578513E-6 5.452315270709643E-5 8.93822175526171E-7 46-47 33.80010231939972 3.664670919657301E-5 2.681466526578513E-6 5.452315270709643E-5 8.93822175526171E-7 48-49 37.964721500160394 3.7093620284336094E-5 2.681466526578513E-6 5.452315270709643E-5 8.93822175526171E-7 50-51 41.99037189053678 3.7540531372099184E-5 2.681466526578513E-6 5.452315270709643E-5 8.93822175526171E-7 52-53 45.77033603879303 3.7540531372099184E-5 2.681466526578513E-6 5.452315270709643E-5 8.93822175526171E-7 54-55 49.361647844127276 3.843435354762536E-5 2.681466526578513E-6 5.452315270709643E-5 1.3407332632892565E-6 56-57 52.76385145723002 3.9328175723151525E-5 2.681466526578513E-6 5.452315270709643E-5 1.787644351052342E-6 58-59 55.942878845881076 3.977508681091461E-5 3.575288702104684E-6 5.7204619233674946E-5 1.787644351052342E-6 60-61 58.962837171064606 4.022199789867769E-5 3.575288702104684E-6 5.899226358472729E-5 1.787644351052342E-6 62-63 61.811132031416385 4.379728660078238E-5 3.575288702104684E-6 6.077990793577963E-5 1.787644351052342E-6 64-65 64.44149695657572 4.379728660078238E-5 3.575288702104684E-6 6.077990793577963E-5 1.787644351052342E-6 66-67 66.89081954081549 4.379728660078238E-5 3.575288702104684E-6 6.077990793577963E-5 1.787644351052342E-6 68-69 69.20099099494716 4.379728660078238E-5 3.575288702104684E-6 6.16737301113058E-5 1.787644351052342E-6 70-71 71.30718060893548 4.379728660078238E-5 3.575288702104684E-6 6.16737301113058E-5 1.787644351052342E-6 72-73 73.26629491231859 4.379728660078238E-5 3.575288702104684E-6 6.16737301113058E-5 1.787644351052342E-6 74-75 75.09513061141897 4.379728660078238E-5 3.575288702104684E-6 6.212064119906889E-5 1.787644351052342E-6 76-77 76.76355159776658 4.379728660078238E-5 3.575288702104684E-6 6.256755228683198E-5 1.787644351052342E-6 78-79 78.32999060226426 4.379728660078238E-5 3.575288702104684E-6 6.301446337459506E-5 1.787644351052342E-6 80-81 79.76623993778568 4.379728660078238E-5 3.575288702104684E-6 6.346137446235814E-5 1.787644351052342E-6 82-83 81.08999057973995 4.379728660078238E-5 3.575288702104684E-6 6.614284098893666E-5 1.787644351052342E-6 84-85 82.30863041531885 4.379728660078238E-5 3.575288702104684E-6 6.793048533998899E-5 1.787644351052342E-6 86-87 83.40017320664882 4.379728660078238E-5 3.575288702104684E-6 6.793048533998899E-5 1.787644351052342E-6 88-89 84.40295185846054 4.379728660078238E-5 3.575288702104684E-6 6.837739642775209E-5 1.787644351052342E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGCT 15880 0.0 32.189133 1 GTTTATT 75590 0.0 31.605785 1 GGCGAAT 11795 0.0 30.857122 1 GCGAATT 11885 0.0 29.488146 2 ACTTAAG 59190 0.0 29.211647 6 GCGGATT 15160 0.0 29.130005 1 CGGATTT 13580 0.0 28.325365 2 GGCGGAT 14120 0.0 27.69928 1 ATCGTCC 4590 0.0 26.278751 6 CGGCGGT 3245 0.0 25.103914 1 TAAGAGG 64475 0.0 24.946423 9 CCGGGCT 12000 0.0 24.772133 1 CGGGTGT 10755 0.0 24.539143 1 GTCGGGT 10585 0.0 24.30317 1 CGGCCGT 4395 0.0 23.846455 1 GCGCGTC 3395 0.0 23.77932 2 CGGGCAT 15505 0.0 23.688776 1 TCGGGTA 8235 0.0 23.585766 2 CGAATTT 14685 0.0 23.283218 3 GTCGGAC 4695 0.0 23.134455 1 >>END_MODULE