FastQCFastQC Report
Fri 27 May 2016
SRR1233076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36150104
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1913230.5292460569408044Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC603860.16704239633722767No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA429770.11888485853318706No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379240.10490702875986194No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA375250.10380329749535436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT210950.053.0279541
AGAGCGT529900.042.7401289
GCGTGTT249650.042.2010732
AAGAGCG557700.041.1717728
GTTTTTT4485250.038.2043841
ATCGGAA608650.037.8969763
GATCGGA609150.037.8502772
AGATCGG619150.037.254291
TCGGAAG622150.036.784534
CGGAAGA634300.036.042485
CGCCGTA371100.030.77067446-47
CCGTATC375200.030.43442748-49
GTCGCCG377500.030.09171544-45
CGTATCA377000.030.03082850-51
GGTCGCC383050.029.50071344-45
TCGCCGT372900.029.39292746-47
CTGGAAT932000.029.0759071
GAAGAGC789850.029.0707047
GCCGTAT390700.028.9838748-49
GTGGTCG382400.028.86767242-43
Produced by FastQC (version 0.11.5)