##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36150104 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.881478930185096 33.0 31.0 34.0 30.0 34.0 2 32.095346945613215 34.0 31.0 34.0 30.0 34.0 3 32.09411353284074 34.0 31.0 34.0 30.0 34.0 4 35.4905291558774 37.0 35.0 37.0 33.0 37.0 5 35.46506881418654 37.0 35.0 37.0 33.0 37.0 6 35.45610643333142 37.0 35.0 37.0 33.0 37.0 7 35.514241591116864 37.0 35.0 37.0 33.0 37.0 8 35.48072461976873 37.0 35.0 37.0 33.0 37.0 9 37.10681446448951 39.0 37.0 39.0 33.0 39.0 10-11 37.03277722243897 39.0 37.0 39.0 33.0 39.0 12-13 36.93539372666812 39.0 37.0 39.0 33.0 39.0 14-15 38.20240114661911 40.0 38.0 41.0 33.0 41.0 16-17 38.01435062261508 40.0 38.0 41.0 33.0 41.0 18-19 37.74760501380577 40.0 38.0 41.0 32.5 41.0 20-21 37.54411739728329 40.0 37.5 41.0 32.0 41.0 22-23 37.286730779529705 40.0 37.0 41.0 32.0 41.0 24-25 37.01392752563035 40.0 37.0 41.0 31.0 41.0 26-27 36.68753337473109 40.0 37.0 41.0 30.0 41.0 28-29 36.56752763422202 40.0 37.0 41.0 30.0 41.0 30-31 36.402210488799696 40.0 37.0 41.0 29.0 41.0 32-33 36.26747650573841 40.0 37.0 41.0 28.0 41.0 34-35 36.15814487006732 40.0 37.0 41.0 27.0 41.0 36-37 36.02907055260478 40.0 37.0 41.0 27.0 41.0 38-39 35.904053968973365 40.0 36.0 41.0 26.5 41.0 40-41 35.76343422137873 40.0 36.0 41.0 26.0 41.0 42-43 35.66022599824332 40.0 36.0 41.0 25.0 41.0 44-45 35.72297969045954 40.0 36.0 41.0 26.0 41.0 46-47 35.62370994838632 40.0 36.0 41.0 25.0 41.0 48-49 35.53128494457443 40.0 36.0 41.0 24.5 41.0 50-51 34.780690188332514 39.0 35.0 40.5 23.5 40.5 52-53 34.820162453751166 39.0 35.0 40.0 24.0 41.0 54-55 35.182172643265424 39.0 35.0 41.0 24.0 41.0 56-57 35.114541593020036 39.0 35.0 41.0 23.5 41.0 58-59 34.95019365642766 39.0 35.0 41.0 23.0 41.0 60-61 34.730148037748386 39.0 35.0 41.0 22.0 41.0 62-63 34.49636352360148 39.0 34.5 41.0 22.0 41.0 64-65 34.20201179780838 38.5 34.0 41.0 20.0 41.0 66-67 33.81485650774338 38.0 34.0 40.0 17.5 41.0 68-69 33.46722410535804 37.5 34.0 40.0 13.5 41.0 70-71 33.13015900313869 37.0 33.5 40.0 12.5 41.0 72-73 32.76138197278769 36.5 33.0 39.0 9.0 41.0 74-75 32.35523561702617 36.0 32.5 39.0 7.5 41.0 76-77 31.952877355484233 35.5 32.0 38.5 6.5 40.0 78-79 31.557873664761793 35.0 32.0 37.5 2.0 39.5 80-81 31.15496723052304 35.0 32.0 37.0 2.0 39.0 82-83 30.765630093346342 35.0 31.0 36.5 2.0 39.0 84-85 30.39635331339572 35.0 31.0 36.0 2.0 37.5 86-87 30.021587794048948 35.0 31.0 36.0 2.0 37.0 88-89 29.63476525544712 35.0 30.5 35.0 2.0 37.0 90-91 29.326586529322295 34.0 30.0 35.0 2.0 36.0 92-93 29.0254101205352 34.0 30.0 35.0 2.0 36.0 94-95 28.702561906875843 34.0 29.5 35.0 2.0 36.0 96-97 28.321217181007277 34.0 29.0 35.0 2.0 35.5 98-99 27.942311078828432 34.0 29.0 35.0 2.0 35.0 100-101 26.523981576927135 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3232630130673755 1101 2 0.34112069389765054 1101 3 0.368929813133974 1101 4 0.38897482293553054 1101 5 0.361799417361226 1101 6 0.39760449533014963 1101 7 0.35618887611882144 1101 8 0.3745510170410853 1101 9 0.4591934416332677 1101 10-11 0.4398879928278774 1101 12-13 0.4633786620344438 1101 14-15 0.5169328857376598 1101 16-17 0.47469994965847917 1101 18-19 0.47336703773753896 1101 20-21 0.48271637396959477 1101 22-23 0.4673499727732988 1101 24-25 0.48168733957170673 1101 26-27 0.3870976121409271 1101 28-29 0.3375175256180256 1101 30-31 0.28494944301098 1101 32-33 0.2672910242766662 1101 34-35 0.199868076008201 1101 36-37 0.1369808135267334 1101 38-39 0.10052506114022464 1101 40-41 0.08637140151865452 1101 42-43 0.07870704500987813 1101 44-45 0.18511345301814686 1101 46-47 0.12376344789458216 1101 48-49 0.09860891361264379 1101 50-51 0.060114497805471956 1101 52-53 0.09085942634974487 1101 54-55 0.11931018898475543 1101 56-57 0.11329695045305499 1101 58-59 0.08310441014035774 1101 60-61 0.020980000083469008 1101 62-63 -0.0010592230098254163 1101 64-65 -0.10286556976937078 1101 66-67 -0.08956418726194215 1101 68-69 -0.03407185224631348 1101 70-71 -0.12488849499126786 1101 72-73 -0.16397302338709352 1101 74-75 -0.24577318528123726 1101 76-77 -0.24680790842587186 1101 78-79 -0.287185905559479 1101 80-81 -0.4214983666966674 1101 82-83 -0.469566760764657 1101 84-85 -0.5163588594261377 1101 86-87 -0.5198659211739987 1101 88-89 -0.5323022774332813 1101 90-91 -0.6114427429460996 1101 92-93 -0.7021164221107163 1101 94-95 -0.7683519166936428 1101 96-97 -0.8982325120127719 1101 98-99 -1.1373724555918514 1101 100-101 -1.2373109327761647 1102 1 0.33354273429588943 1102 2 0.3309909526552275 1102 3 0.3585270270877814 1102 4 0.27698526651057875 1102 5 0.344803345485289 1102 6 0.33681524385644224 1102 7 0.33651828307264964 1102 8 0.3033840521911415 1102 9 0.386625048588094 1102 10-11 0.3890855849052173 1102 12-13 0.3871705142987878 1102 14-15 0.44998948437331876 1102 16-17 0.4239969157573711 1102 18-19 0.49621198321995763 1102 20-21 0.4619117522024112 1102 22-23 0.450198570932379 1102 24-25 0.44688236477324494 1102 26-27 0.41040944734311324 1102 28-29 0.39641992553990235 1102 30-31 0.37032279782246036 1102 32-33 0.31100202905562213 1102 34-35 0.2837867277917283 1102 36-37 0.3017956697790609 1102 38-39 0.26246268160446107 1102 40-41 0.3069446452684801 1102 42-43 0.2996974891732336 1102 44-45 0.3680077442206411 1102 46-47 0.41410225278415425 1102 48-49 0.30361472726146843 1102 50-51 0.32531926560015023 1102 52-53 0.31971609169547577 1102 54-55 0.33622453840279576 1102 56-57 0.26911948536052677 1102 58-59 0.2652702824346278 1102 60-61 0.28955545964275586 1102 62-63 0.20782406741793835 1102 64-65 0.2833537388241396 1102 66-67 0.31207020711085676 1102 68-69 0.2975911736036281 1102 70-71 0.27840602109275636 1102 72-73 0.19269716272707882 1102 74-75 0.132477294554171 1102 76-77 0.15040482327148297 1102 78-79 0.1063800717896477 1102 80-81 0.015370956527728907 1102 82-83 0.0327734234523156 1102 84-85 0.00496714502375184 1102 86-87 -0.05806588453765826 1102 88-89 -0.052279859185343724 1102 90-91 -0.047280423129631544 1102 92-93 -0.16777624662265467 1102 94-95 -0.22674814862392978 1102 96-97 -0.30004107145930803 1102 98-99 -0.33867622922670293 1102 100-101 -0.49518792660625266 1103 1 0.2781278145535069 1103 2 0.30221140542236213 1103 3 0.3485602717465355 1103 4 0.3144666383875503 1103 5 0.34311581189162865 1103 6 0.32322801076236374 1103 7 0.3161969383780061 1103 8 0.3239274632945808 1103 9 0.3964432576852417 1103 10-11 0.4132358526190316 1103 12-13 0.423350836822749 1103 14-15 0.4727742215276436 1103 16-17 0.44141579074562287 1103 18-19 0.4292868694654217 1103 20-21 0.39132906453298233 1103 22-23 0.4104909326474697 1103 24-25 0.3822542443624144 1103 26-27 0.3988414590203533 1103 28-29 0.3242435909715766 1103 30-31 0.3271184552854649 1103 32-33 0.33487351219910266 1103 34-35 0.37906260806325776 1103 36-37 0.3773919416469482 1103 38-39 0.35142356547738274 1103 40-41 0.3249019878753643 1103 42-43 0.37681479142330687 1103 44-45 0.3763548089632778 1103 46-47 0.38040793473832224 1103 48-49 0.3077982535431758 1103 50-51 0.11533908798840287 1103 52-53 0.29359229200034775 1103 54-55 0.36779849877292037 1103 56-57 0.3698139977665207 1103 58-59 0.3622752536970779 1103 60-61 0.32963885835027895 1103 62-63 0.37913503904379553 1103 64-65 0.34018585369946663 1103 66-67 0.2443240962082598 1103 68-69 0.2755600178895108 1103 70-71 0.19637034533444364 1103 72-73 0.20462926318735697 1103 74-75 0.19951290120796017 1103 76-77 0.17595715360844721 1103 78-79 0.10147564608540804 1103 80-81 0.052182733186956654 1103 82-83 0.016173370016435484 1103 84-85 -0.06783947037497384 1103 86-87 -0.07834596974641883 1103 88-89 -0.10982980464907044 1103 90-91 -0.1643697260529393 1103 92-93 -0.20762133457265364 1103 94-95 -0.23679976905097888 1103 96-97 -0.21400894636041912 1103 98-99 -0.33747793649541435 1103 100-101 -0.528524866005565 1104 1 0.19156832594283202 1104 2 0.19367429123297342 1104 3 0.22527388304884255 1104 4 0.21199042155935643 1104 5 0.19158068766893166 1104 6 0.21389191937216623 1104 7 0.21642889638513196 1104 8 0.27324747229643265 1104 9 0.2702665328327285 1104 10-11 0.28366603476379737 1104 12-13 0.2659551007880623 1104 14-15 0.23816725140916617 1104 16-17 0.2748252507151605 1104 18-19 0.3071187189937916 1104 20-21 0.2590129014923974 1104 22-23 0.316976833803416 1104 24-25 0.3222058262460479 1104 26-27 0.3074422015142986 1104 28-29 0.32441656019215515 1104 30-31 0.2703971488510817 1104 32-33 0.21758465608612454 1104 34-35 0.19280420041352642 1104 36-37 0.24900322231552963 1104 38-39 0.20844718423175124 1104 40-41 0.1650908957570394 1104 42-43 0.18492619086360662 1104 44-45 0.24785606689668072 1104 46-47 0.23537633713716133 1104 48-49 0.20515658642896284 1104 50-51 0.08870182567703466 1104 52-53 0.22272012166262556 1104 54-55 0.26853371857508535 1104 56-57 0.3027959395106521 1104 58-59 0.27894246723366933 1104 60-61 0.2104597359047773 1104 62-63 0.17693367543527216 1104 64-65 0.20058671770468095 1104 66-67 0.12856128543400303 1104 68-69 0.24611614275032423 1104 70-71 0.17604417232296754 1104 72-73 0.17574160090469348 1104 74-75 0.15414207618478315 1104 76-77 0.1384491588501966 1104 78-79 0.1558137402093145 1104 80-81 0.14242582381703528 1104 82-83 0.07160356783891686 1104 84-85 0.002907404684481918 1104 86-87 0.0031404901536866703 1104 88-89 0.04467846331180425 1104 90-91 -0.005938880705546268 1104 92-93 -0.04109330212640927 1104 94-95 -0.04175547699446014 1104 96-97 -0.037558095121706714 1104 98-99 -0.10930747695766385 1104 100-101 -0.2344009566488765 1105 1 0.1588047269403603 1105 2 0.1983524780115502 1105 3 0.2177931875095993 1105 4 0.173110095631003 1105 5 0.21921729973472992 1105 6 0.22610441570797235 1105 7 0.18959541722340134 1105 8 0.16048894315168383 1105 9 0.21601027642245185 1105 10-11 0.22543432238068561 1105 12-13 0.18660385473162222 1105 14-15 0.2612649373583764 1105 16-17 0.24696352425849 1105 18-19 0.25116942951374455 1105 20-21 0.12704049681855167 1105 22-23 0.20986376795766404 1105 24-25 0.27352214187196466 1105 26-27 0.27534541850505434 1105 28-29 0.2390459289079132 1105 30-31 0.25395042823428327 1105 32-33 0.17759201425818105 1105 34-35 0.16618382471858695 1105 36-37 0.19883173780166885 1105 38-39 0.13652780230918182 1105 40-41 0.12476594164751731 1105 42-43 0.15755840251949138 1105 44-45 0.21025441943827872 1105 46-47 0.1727286900283147 1105 48-49 0.18000978230637088 1105 50-51 0.05851841406914815 1105 52-53 0.08985707760862738 1105 54-55 0.1408596335795167 1105 56-57 0.1254741099898169 1105 58-59 0.2120628711563768 1105 60-61 0.14488044818396872 1105 62-63 0.17764738042995276 1105 64-65 0.2047437412599109 1105 66-67 0.19941112235241576 1105 68-69 0.17654252156437167 1105 70-71 0.15661210008711635 1105 72-73 0.17363040706709398 1105 74-75 0.199827441231065 1105 76-77 0.17553683338747916 1105 78-79 0.2258236709262853 1105 80-81 0.1498857676145633 1105 82-83 0.13251423400636853 1105 84-85 0.04262369302657021 1105 86-87 0.11471888964803512 1105 88-89 0.03027223538920154 1105 90-91 -0.031947108199030794 1105 92-93 -0.0494609173816265 1105 94-95 0.0267320638514299 1105 96-97 0.022457497689575945 1105 98-99 9.143971836778064E-4 1105 100-101 -0.04842712794917148 1106 1 0.03719988823832665 1106 2 0.09460908949426994 1106 3 0.07254139946310545 1106 4 0.12756742274124377 1106 5 0.11475158104529015 1106 6 0.10803098395825117 1106 7 0.12280323301199303 1106 8 0.18618849255119585 1106 9 0.1567680860216143 1106 10-11 0.13762702972847762 1106 12-13 0.012061969937036565 1106 14-15 0.07438248538924341 1106 16-17 0.1022929700882571 1106 18-19 0.04949571408254627 1106 20-21 0.08686616423356952 1106 22-23 0.10580062244957134 1106 24-25 0.1067683551058849 1106 26-27 0.16909199081040072 1106 28-29 0.14941556696334146 1106 30-31 0.1332358114228427 1106 32-33 0.1674489490865696 1106 34-35 0.07592885109225733 1106 36-37 0.07599050769262305 1106 38-39 0.11052971356381391 1106 40-41 0.07141808886213852 1106 42-43 0.02531200118989574 1106 44-45 0.12820035337882274 1106 46-47 0.1517958897032301 1106 48-49 0.0802161782394748 1106 50-51 0.051060130212356114 1106 52-53 0.07689386478086391 1106 54-55 0.14533917313043077 1106 56-57 0.1710810126143656 1106 58-59 0.13513756973046043 1106 60-61 0.13417487058754318 1106 62-63 0.12417517940819778 1106 64-65 0.09024893673697676 1106 66-67 0.1607941997616109 1106 68-69 0.14973608337614053 1106 70-71 0.17267371742406823 1106 72-73 0.18520055736794205 1106 74-75 0.09212272981133651 1106 76-77 0.08196333737243222 1106 78-79 0.11866818344391206 1106 80-81 0.06261830442505811 1106 82-83 0.12233913926011653 1106 84-85 0.014525598639362869 1106 86-87 0.08421591529231875 1106 88-89 0.05380331790998483 1106 90-91 0.1007044177309453 1106 92-93 0.05211777677358498 1106 94-95 0.08084382426248737 1106 96-97 0.007046792881698849 1106 98-99 0.002590858673197971 1106 100-101 -0.1338242708909867 1107 1 -0.06260812876840305 1107 2 -0.02422909532578643 1107 3 0.015974168751689888 1107 4 0.08737633178593995 1107 5 0.06869008159211631 1107 6 0.09280935006981395 1107 7 0.0730741641401238 1107 8 0.09922342359666914 1107 9 0.017072528456438363 1107 10-11 0.10018143298622562 1107 12-13 0.06082945999970235 1107 14-15 0.06662317261459805 1107 16-17 0.07084098492518365 1107 18-19 0.07220855448752417 1107 20-21 0.0233372186877574 1107 22-23 -0.048780641082750265 1107 24-25 0.017857990461024542 1107 26-27 0.04601978731616185 1107 28-29 0.07503875107760649 1107 30-31 0.05634567559064152 1107 32-33 0.04397818548302013 1107 34-35 0.020169086039146578 1107 36-37 -0.014480698959161487 1107 38-39 -0.05820335551043598 1107 40-41 -0.027575074556473567 1107 42-43 -0.053318078615426145 1107 44-45 -0.04509629448768493 1107 46-47 -0.10130911457836334 1107 48-49 -0.10210945580801223 1107 50-51 -0.12709125413652345 1107 52-53 -0.10829215436062611 1107 54-55 -0.005636019264279923 1107 56-57 0.030510505172159696 1107 58-59 0.017899536470814326 1107 60-61 0.020758323509483034 1107 62-63 -0.031749903355091647 1107 64-65 -0.03546215345496506 1107 66-67 -0.025724721152130314 1107 68-69 0.062254390050526354 1107 70-71 0.0020299979978020133 1107 72-73 0.01192370962089484 1107 74-75 0.034545649365391284 1107 76-77 0.00952103687819772 1107 78-79 0.03607850992871775 1107 80-81 0.040251954031305104 1107 82-83 -0.028084739004082593 1107 84-85 -7.10907744046807E-4 1107 86-87 -0.0396114694498273 1107 88-89 -0.03299251835502304 1107 90-91 -0.10028590994459918 1107 92-93 -0.13394697661066246 1107 94-95 -0.15535244845585439 1107 96-97 -0.19847071010750383 1107 98-99 -0.1826203854168007 1107 100-101 -0.25533125417265623 1108 1 -0.05963580619373232 1108 2 -0.03603500929531833 1108 3 -0.05493556880129091 1108 4 -0.023112681952071057 1108 5 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-1.1313329471634432 2316 84-85 -1.1009589379127966 2316 86-87 -1.067713794965929 2316 88-89 -1.03714364116259 2316 90-91 -1.05870062346332 2316 92-93 -1.2087099232371408 2316 94-95 -1.0514922836781757 2316 96-97 -1.2573508574808834 2316 98-99 -1.2851799271121216 2316 100-101 -1.3061447945208506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 188999.0 3 45419.0 4 40509.0 5 47922.0 6 75565.0 7 130217.0 8 209984.0 9 322750.0 10 374943.0 11 308261.0 12 216325.0 13 170323.0 14 147954.0 15 135564.0 16 133520.0 17 136053.0 18 142792.0 19 153847.0 20 170076.0 21 191282.0 22 217142.0 23 248751.0 24 289718.0 25 302784.0 26 316257.0 27 365285.0 28 430148.0 29 508602.0 30 610750.0 31 746080.0 32 928274.0 33 1185733.0 34 1577330.0 35 2228308.0 36 3448822.0 37 6245053.0 38 9566912.0 39 3583396.0 40 8454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.550175567959638 16.45261380160898 10.340407319436757 48.656803310994626 2 15.004454758968327 18.63441388716337 55.446545879923335 10.914585473944971 3 18.271632081611717 22.800800241127934 37.50104010765778 21.426527569602566 4 19.120039046084074 30.34874256516662 33.27017537764207 17.261043011107244 5 19.016885815874833 31.540354628025412 33.81841999680001 15.624339559299747 6 15.697232848901347 33.30857360742309 35.56922823790493 15.424965305770627 7 13.736729498758843 17.077184618887955 51.54146444502622 17.644621437326986 8 13.565421554527202 21.811992574073923 44.76033595919945 19.862249912199424 9 16.632848414488656 20.892565067032727 42.55613759783374 19.918448920644877 10-11 20.076292173322656 31.81947277385426 31.026578789372223 17.077656263450862 12-13 18.008985534315475 25.939486923744397 38.08540633797347 17.96612120396666 14-15 18.385921061954917 26.40613053062158 37.665249440841556 17.542698966581945 16-17 19.21281609590943 26.943037286974338 35.32877111501533 18.515375502100905 18-19 20.35377685220491 27.342872927834456 33.86502013936115 18.438330080599492 20-21 19.576878672326917 28.612606481021462 33.4709548830067 18.33955996364492 22-23 20.132883988383547 28.538339751387714 34.04585502713907 17.282921233089677 24-25 19.535929965789308 28.869157610168976 33.56068629844052 18.034226125601187 26-27 19.387256811211387 29.292513238689438 32.74628200239756 18.573947947701615 28-29 20.010438974117477 29.45483918939763 31.185445552245163 19.349276284239735 30-31 19.505905155902177 28.71694228044268 32.0041472079859 19.77300535566924 32-33 20.00307661632177 29.214299079194905 31.85369674178531 18.92892756269802 34-35 19.15464306026953 30.22995152853463 30.934771108014107 19.680634303181726 36-37 19.1169685154986 29.28100566460334 31.69917159851048 19.902854221387578 38-39 19.7647398192824 28.52088337007274 31.896310727072873 19.818066083571985 40-41 20.955076643762908 28.655144394605337 30.862938872872952 19.526840088758803 42-43 20.57031288208742 29.22777068635819 31.106138726461204 19.095777705093187 44-45 20.58637369342008 29.080260737285847 30.93688886759496 19.396476701699115 46-47 19.697514563166955 28.89243001901184 31.137048734354956 20.27300668346625 48-49 20.788463015210137 29.282312714784997 29.78645234326297 20.142771926741897 50-51 20.177941950042523 29.15073632983186 30.709502246521893 19.96181947360373 52-53 19.714716450055025 29.83472191393972 31.354049493190946 19.096512142814305 54-55 19.833514171909435 29.589592605321414 30.83939675526245 19.737496467506705 56-57 20.263114595742245 28.986283137664003 30.946913735019958 19.803688531573798 58-59 20.098534156360934 29.1645993604887 30.299316704593714 20.437549778556654 60-61 20.519282600127514 29.92364420307062 29.90000250068437 19.657070696117497 62-63 19.255254167198995 31.492682390582605 30.018948914693617 19.233114527524776 64-65 19.125294909248396 30.64014698270301 30.58772804637021 19.646830061678383 66-67 19.47453871778626 29.63370174536704 30.884918062753012 20.006841474093683 68-69 20.635983509203736 30.137271527628247 29.32531674044423 19.901428222723787 70-71 20.049330978411568 30.558912085010874 29.42264841063804 19.96910852593951 72-73 19.55993127986575 30.599633959559284 30.149336499834135 19.691098260740826 74-75 19.95875032614014 29.5511929924185 30.77952970757705 19.710526973864305 76-77 20.35222360632766 29.78698207894506 29.925604086782158 19.935190227945128 78-79 20.185105138286737 30.559109871440484 29.204709895163788 20.051075095108995 80-81 20.12785910657408 30.10226886207575 30.610658824107396 19.15921320724278 82-83 19.827371727616608 30.36777293918712 30.243830003919214 19.561025329277058 84-85 19.86615723152553 30.12135317784978 29.9496524270027 20.062837163621992 86-87 20.574337766773784 30.185647598690174 29.25477060868207 19.98524402585398 88-89 20.05351630523663 29.992323120287566 29.812611327480553 20.14154924699525 90-91 20.08164208877518 30.218159261727155 29.594953585749018 20.10524506374864 92-93 19.678911573809028 30.592716967010663 29.61475712490343 20.113614334276882 94-95 19.49315692148493 30.45348085305647 29.46270362043772 20.590658605020888 96-97 19.831846126915707 29.834748193255546 29.871297189075857 20.46210849075289 98-99 20.354764401231044 30.144394605337787 29.38618793461839 20.114653058812777 100-101 20.722927934038584 30.39855569986742 28.64635188878018 20.232164477313813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1875.0 1 7432.0 2 10180.0 3 8446.5 4 10627.0 5 13641.5 6 16493.0 7 19234.0 8 23108.0 9 27021.0 10 31013.5 11 35367.0 12 40190.5 13 44987.5 14 50252.5 15 56157.5 16 62943.0 17 71107.0 18 81387.5 19 95498.0 20 113642.0 21 138343.0 22 169385.0 23 208208.0 24 257833.0 25 319482.0 26 392773.5 27 480713.5 28 591062.5 29 702698.5 30 840774.5 31 999151.5 32 1208556.0 33 1440647.5 34 1632649.0 35 1817848.0 36 1947007.5 37 1932061.5 38 1762198.5 39 1547440.5 40 1408856.0 41 1323065.5 42 1253100.0 43 1217097.0 44 1170143.5 45 1112342.0 46 1058454.0 47 995764.0 48 930762.0 49 863207.5 50 791703.5 51 720679.5 52 644538.0 53 571300.0 54 504368.0 55 444207.0 56 384064.5 57 317851.0 58 259356.5 59 211840.0 60 166146.0 61 127788.5 62 98389.5 63 74457.0 64 57441.0 65 43944.5 66 32502.5 67 24354.5 68 18422.0 69 13972.0 70 11637.0 71 11540.0 72 10601.5 73 11526.5 74 11066.0 75 7588.0 76 6636.5 77 6353.5 78 5874.5 79 4895.5 80 3725.5 81 2739.0 82 2162.0 83 1652.0 84 1061.5 85 665.5 86 494.5 87 332.5 88 243.0 89 192.5 90 136.5 91 106.0 92 83.5 93 58.5 94 42.5 95 31.0 96 17.0 97 10.5 98 6.0 99 4.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.3941753528565226E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.005526733754348E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.457804713369566E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.6150104E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07583044969866 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43010903750094 37.225167937885736 2 10.938034113213128 10.517101469597085 3 4.153419896015846 5.990373321217885 4 2.080958456493873 4.001752237090642 5 1.2366986496112584 2.9727657298041072 6 0.8346339081065527 2.407543095222 7 0.5736773237375491 1.9306009624190303 8 0.4367716096061883 1.6798526278935262 9 0.3245224022789092 1.4041515590180766 >10 1.8017843781327054 15.58467773509019 >50 0.11750273600783885 3.8714060560243078 >100 0.06349183901375237 5.632442016273364 >500 0.005193888317732507 1.7141839079324754 >1k 0.002867800951120936 2.648629467576622 >5k 2.3031794249799071E-4 0.745429772094097 >10k+ 1.0364306980580694E-4 1.673922104860986 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 191323 0.5292460569408044 Illumina Single End PCR Primer 1 (100% over 50bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 60386 0.16704239633722767 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 42977 0.11888485853318706 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37924 0.10490702875986194 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 37525 0.10380329749535436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.6673784396304918 0.0 0.0 0.0 0.0 2 0.6801391221447108 1.1064975082782612E-5 0.0 1.936370639486957E-5 0.0 3 0.681419893010543 1.1064975082782612E-5 0.0 2.4896193936260877E-5 0.0 4 0.6864295604792727 1.1064975082782612E-5 0.0 3.3194925248347835E-5 0.0 5 0.6879869557221744 1.1064975082782612E-5 0.0 3.872741278973914E-5 2.766243770695653E-6 6 0.6905374324787558 1.3831218853478263E-5 0.0 4.70261441018261E-5 2.766243770695653E-6 7 0.6914779553607924 1.3831218853478263E-5 0.0 4.70261441018261E-5 2.766243770695653E-6 8 0.6934668846319225 1.6597462624173918E-5 0.0 5.532487541391305E-5 2.766243770695653E-6 9 0.6959399065629244 1.6597462624173918E-5 0.0 6.085736295530436E-5 2.766243770695653E-6 10-11 0.7044820673268326 1.6597462624173918E-5 0.0 7.053921615273915E-5 2.766243770695653E-6 12-13 0.7174156400767201 1.936370639486957E-5 0.0 7.468858180878263E-5 2.766243770695653E-6 14-15 0.7344377211196957 2.0746828280217397E-5 0.0 7.883794746482611E-5 2.766243770695653E-6 16-17 0.755291049785085 2.2129950165565224E-5 0.0 9.128604443295655E-5 2.766243770695653E-6 18-19 0.7767543352019126 2.2129950165565224E-5 0.0 9.681853197434785E-5 5.532487541391306E-6 20-21 0.7977501254214925 2.2129950165565224E-5 0.0 1.0788350705713045E-4 9.681853197434785E-6 22-23 0.8170239288938145 2.2129950165565224E-5 0.0 1.1479911648386958E-4 1.3831218853478263E-5 24-25 0.8330142010103208 2.2129950165565224E-5 0.0 1.2309784779595656E-4 1.3831218853478263E-5 26-27 0.8468315886449456 2.2129950165565224E-5 0.0 1.4937716361756526E-4 1.3831218853478263E-5 28-29 0.8594345952642349 2.2129950165565224E-5 2.766243770695653E-6 1.7150711378313048E-4 1.6597462624173918E-5 30-31 0.8710154748102523 2.2129950165565224E-5 2.766243770695653E-6 2.0885140468752177E-4 1.936370639486957E-5 32-33 0.8809338418500815 2.4896193936260877E-5 2.766243770695653E-6 2.2268262354100004E-4 2.0746828280217397E-5 34-35 0.891362580865604 2.4896193936260877E-5 2.766243770695653E-6 2.3374759862378265E-4 2.2129950165565224E-5 36-37 0.9018452616346553 2.4896193936260877E-5 2.766243770695653E-6 2.5864379256004355E-4 2.2129950165565224E-5 38-39 0.9118037392091596 2.4896193936260877E-5 2.766243770695653E-6 2.71091889528174E-4 2.2129950165565224E-5 40-41 0.9214828261628238 2.4896193936260877E-5 2.766243770695653E-6 2.807737427256088E-4 2.351307205091305E-5 42-43 0.9314302387622453 2.7662437706956526E-5 2.766243770695653E-6 2.8907247403769573E-4 2.766243770695653E-5 44-45 0.9414440412121636 2.7662437706956526E-5 2.766243770695653E-6 3.0013744912047835E-4 3.042868147765218E-5 46-47 0.951347193911254 2.7662437706956526E-5 2.766243770695653E-6 3.02903692891174E-4 3.3194925248347835E-5 48-49 0.9618672189712096 2.7662437706956526E-5 2.766243770695653E-6 3.125855460886088E-4 3.3194925248347835E-5 50-51 0.9724093739813308 2.9045559592304353E-5 2.766243770695653E-6 3.181180336300001E-4 3.457804713369566E-5 52-53 0.9824923325255164 3.3194925248347835E-5 2.766243770695653E-6 3.2641676494208705E-4 3.596116901904349E-5 54-55 0.9930289550480962 3.596116901904349E-5 2.766243770695653E-6 3.3748174002486966E-4 3.7344290904391315E-5 56-57 1.0038062407787265 3.596116901904349E-5 2.766243770695653E-6 3.402479837955653E-4 4.1493656560434794E-5 58-59 1.0145226691464013 3.596116901904349E-5 2.766243770695653E-6 3.4716359322230445E-4 4.1493656560434794E-5 60-61 1.0254410333093371 3.7344290904391315E-5 2.766243770695653E-6 3.554623245343914E-4 4.1493656560434794E-5 62-63 1.0372736410384877 4.1493656560434794E-5 2.766243770695653E-6 3.596116901904349E-4 4.425990033113045E-5 64-65 1.0496525819123508 4.1493656560434794E-5 2.766243770695653E-6 3.66527299617174E-4 4.425990033113045E-5 66-67 1.0625792390528117 4.425990033113045E-5 2.766243770695653E-6 3.7344290904391315E-4 4.70261441018261E-5 68-69 1.0768074138873847 4.425990033113045E-5 2.766243770695653E-6 3.803585184706523E-4 4.979238787252175E-5 70-71 1.092479567970261 4.979238787252175E-5 2.766243770695653E-6 3.969559810948262E-4 4.979238787252175E-5 72-73 1.1087132695385884 4.979238787252175E-5 2.766243770695653E-6 4.011053467508697E-4 5.394175352856523E-5 74-75 1.1265500093720338 5.25586316432174E-5 2.766243770695653E-6 4.107871999483044E-4 5.8091119184608705E-5 76-77 1.1466854977789276 5.532487541391305E-5 2.766243770695653E-6 4.2876778445782615E-4 5.947424106995653E-5 78-79 1.168895669013843 6.085736295530436E-5 5.532487541391306E-6 4.3430027199921746E-4 6.224048484065219E-5 80-81 1.1943506441917844 6.085736295530436E-5 5.532487541391306E-6 4.370665157699131E-4 6.362360672600002E-5 82-83 1.223365775102611 6.085736295530436E-5 5.532487541391306E-6 4.508977346233914E-4 6.362360672600002E-5 84-85 1.25926885300247 6.085736295530436E-5 5.532487541391306E-6 4.6057958782082616E-4 6.77729723820435E-5 86-87 1.3038136764419819 6.085736295530436E-5 5.532487541391306E-6 4.61962709706174E-4 7.192233803808698E-5 88-89 1.3544732817366167 6.085736295530436E-5 5.532487541391306E-6 4.716445629036088E-4 7.192233803808698E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 21095 0.0 53.027954 1 AGAGCGT 52990 0.0 42.740128 9 GCGTGTT 24965 0.0 42.201073 2 AAGAGCG 55770 0.0 41.171772 8 GTTTTTT 448525 0.0 38.204384 1 ATCGGAA 60865 0.0 37.896976 3 GATCGGA 60915 0.0 37.850277 2 AGATCGG 61915 0.0 37.25429 1 TCGGAAG 62215 0.0 36.78453 4 CGGAAGA 63430 0.0 36.04248 5 CGCCGTA 37110 0.0 30.770674 46-47 CCGTATC 37520 0.0 30.434427 48-49 GTCGCCG 37750 0.0 30.091715 44-45 CGTATCA 37700 0.0 30.030828 50-51 GGTCGCC 38305 0.0 29.500713 44-45 TCGCCGT 37290 0.0 29.392927 46-47 CTGGAAT 93200 0.0 29.075907 1 GAAGAGC 78985 0.0 29.070704 7 GCCGTAT 39070 0.0 28.98387 48-49 GTGGTCG 38240 0.0 28.867672 42-43 >>END_MODULE