FastQCFastQC Report
Fri 27 May 2016
SRR1233076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36150104
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1969140.5447121258627637TruSeq Adapter, Index 5 (100% over 49bp)
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC646570.1788570234818688No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA440530.12186133683045558No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA384030.10623205952602516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT191950.056.6887471
GCGTGTT223650.045.7842332
CGGAAGA667300.035.825285
AGATCGG596050.035.506371
TCGGAAG634100.035.3189434
GATCGGA609850.035.092392
ATCGGAA612050.035.0241973
GTTCGGA28050.034.7263032
GTTTTTT3606750.031.2705881
CGTGTTT347350.029.1957953
CTGGAAT958800.028.8387171
GAAGAGC841700.028.4397457
AGAGCAC852500.028.1630239
TATGCCG506650.027.51203346-47
TGCCGTC510350.027.20195848-49
CCGTCTT525200.026.3750250-51
CGTATGC533100.026.24431844-45
CTCGTAT523500.026.18542-43
ATCTCGT529600.025.42624540-41
AGTTCGG37200.025.313441
Produced by FastQC (version 0.11.5)