##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233076_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36150104 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870266790933712 33.0 31.0 34.0 30.0 34.0 2 32.164987962413605 34.0 31.0 34.0 30.0 34.0 3 30.716921091015394 34.0 31.0 34.0 25.0 34.0 4 34.17231718060894 37.0 35.0 37.0 28.0 37.0 5 35.09874242685443 37.0 35.0 37.0 32.0 37.0 6 35.51532194762151 37.0 35.0 37.0 33.0 37.0 7 35.75814019788159 37.0 35.0 37.0 35.0 37.0 8 35.840889752350364 37.0 35.0 37.0 35.0 37.0 9 37.638604082577466 39.0 37.0 39.0 35.0 39.0 10-11 37.610290111475194 39.0 37.0 39.0 35.0 39.0 12-13 37.57155303066348 39.0 37.0 39.0 35.0 39.0 14-15 38.97051441401109 40.0 38.0 41.0 35.5 41.0 16-17 38.874940940695495 40.0 38.0 41.0 35.0 41.0 18-19 38.72817826471537 40.0 38.0 41.0 34.5 41.0 20-21 38.63643892974692 40.0 38.0 41.0 34.0 41.0 22-23 38.472328807131504 40.0 38.0 41.0 34.0 41.0 24-25 38.28115260747244 40.0 38.0 41.0 34.0 41.0 26-27 37.93185693186388 40.0 38.0 41.0 33.0 41.0 28-29 37.827549956702754 40.0 38.0 41.0 33.0 41.0 30-31 37.69387728455774 40.0 38.0 41.0 33.0 41.0 32-33 37.58648919792873 40.0 38.0 41.0 33.0 41.0 34-35 37.48144938946787 40.0 38.0 41.0 32.0 41.0 36-37 37.37920019538533 40.0 38.0 41.0 32.0 41.0 38-39 37.23430613090352 40.0 38.0 41.0 31.0 41.0 40-41 37.13659144659722 40.0 37.5 41.0 31.0 41.0 42-43 36.999486350025435 40.0 37.0 41.0 31.0 41.0 44-45 37.07711067719197 40.0 37.5 41.0 31.0 41.0 46-47 37.06109968590962 40.0 37.5 41.0 31.0 41.0 48-49 36.98663472724726 40.0 37.0 41.0 31.0 41.0 50-51 36.89548819831887 40.0 37.0 41.0 31.0 41.0 52-53 36.76856127440187 40.0 37.0 41.0 31.0 41.0 54-55 36.59169875417233 40.0 37.0 41.0 30.0 41.0 56-57 36.44014068396595 40.0 36.0 41.0 30.0 41.0 58-59 36.28742507905371 40.0 36.0 41.0 29.5 41.0 60-61 36.05311795230243 39.0 36.0 41.0 29.0 41.0 62-63 35.83717600093211 39.0 35.0 41.0 28.5 41.0 64-65 35.562103292980844 39.0 35.0 41.0 28.0 41.0 66-67 35.27698238433837 38.0 35.0 40.0 28.0 41.0 68-69 34.9159558157841 38.0 34.5 40.0 28.0 41.0 70-71 34.4558960881551 37.0 34.0 40.0 26.0 41.0 72-73 34.034750508601576 37.0 34.0 39.0 26.0 41.0 74-75 33.61118660958762 36.0 34.0 39.0 26.0 41.0 76-77 32.41987218072734 35.0 31.5 37.5 24.0 39.0 78-79 32.81455505632847 35.0 33.0 37.5 25.0 39.0 80-81 32.60993355648438 35.0 33.0 37.0 25.0 39.0 82-83 32.23820524831685 35.0 33.0 37.0 24.0 39.0 84-85 31.890064023052325 35.0 33.0 36.0 23.0 37.5 86-87 31.58211064897628 35.0 33.0 36.0 22.0 37.0 88-89 31.24823775057466 35.0 32.0 35.5 20.0 37.0 90-91 30.961297843015885 35.0 32.0 35.0 19.5 36.0 92-93 30.711635048131534 35.0 32.0 35.0 18.0 36.0 94-95 30.47643691426171 35.0 32.0 35.0 11.5 36.0 96-97 30.221455752935043 34.5 32.0 35.0 2.0 36.0 98-99 29.89317051480682 34.0 31.5 35.0 2.0 35.0 100-101 28.611010261547243 33.5 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5886205300606022 1101 2 0.46842260922628753 1101 3 2.022266687465894 1101 4 2.018055381765535 1101 5 1.0363180040756603 1101 6 0.6387038303047632 1101 7 0.4244923408056991 1101 8 0.3186975130218528 1101 9 0.34086011315274334 1101 10-11 0.33362416297429576 1101 12-13 0.3120056829622584 1101 14-15 0.3605008299609551 1101 16-17 0.3692451326678352 1101 18-19 0.36610424114346074 1101 20-21 0.3609201395768551 1101 22-23 0.37295117971772385 1101 24-25 0.3553333980771427 1101 26-27 0.39002648785683647 1101 28-29 0.3125082616033552 1101 30-31 0.2512171359428308 1101 32-33 0.2531631536335439 1101 34-35 0.26326419148950464 1101 36-37 0.22913778446668687 1101 38-39 0.23263052904010095 1101 40-41 0.19514041664889703 1101 42-43 0.12681307032772082 1101 44-45 0.2162620449685022 1101 46-47 0.2888995445118425 1101 48-49 0.33036749147534294 1101 50-51 0.3085289125167492 1101 52-53 0.21513687733170883 1101 54-55 0.21273291533407956 1101 56-57 0.21564383423773137 1101 58-59 0.1939701984866602 1101 60-61 0.09547308791911746 1101 62-63 0.18088349095018685 1101 64-65 0.010872364740237117 1101 66-67 0.01615107354778189 1101 68-69 0.07416305566341919 1101 70-71 0.13513380181811385 1101 72-73 0.043330652516765156 1101 74-75 0.06354759462016801 1101 76-77 0.06366428762939336 1101 78-79 0.11879799925839052 1101 80-81 0.13820228990951477 1101 82-83 0.13064088740623703 1101 84-85 0.06557455572408699 1101 86-87 0.02754751274362377 1101 88-89 -0.007140814439438259 1101 90-91 -0.011503659631738827 1101 92-93 -0.03462577244262377 1101 94-95 -0.12268951748681545 1101 96-97 -0.20605085368278608 1101 98-99 -0.2739551204431372 1101 100-101 -0.33416737556778386 1102 1 0.5748631144289931 1102 2 0.44965512812051855 1102 3 1.9795041592653035 1102 4 2.0123391251987712 1102 5 1.055594911518277 1102 6 0.6279650178382923 1102 7 0.4345297765484517 1102 8 0.30630171292790465 1102 9 0.3573372829855046 1102 10-11 0.3447431988691605 1102 12-13 0.30364837472995276 1102 14-15 0.36232535181018477 1102 16-17 0.35376448210146094 1102 18-19 0.2804133797757089 1102 20-21 0.35185683307155813 1102 22-23 0.3608129881469182 1102 24-25 0.36234600686638885 1102 26-27 0.3377471766362561 1102 28-29 0.3151116185663625 1102 30-31 0.28983829690104557 1102 32-33 0.33487257172751583 1102 34-35 0.3195964698149609 1102 36-37 0.2827217820015804 1102 38-39 0.27405184997492427 1102 40-41 0.2596303997178353 1102 42-43 0.23983036443136996 1102 44-45 0.32720026320718887 1102 46-47 0.3373953601473545 1102 48-49 0.3761353290004976 1102 50-51 0.3648838792824378 1102 52-53 0.2770472554463339 1102 54-55 0.26243520017999344 1102 56-57 0.2686573856822392 1102 58-59 0.2738255504829681 1102 60-61 0.28728028633469904 1102 62-63 0.312031588065075 1102 64-65 0.2839893105868896 1102 66-67 0.2413557156730448 1102 68-69 0.30800465413109634 1102 70-71 0.26665759110066745 1102 72-73 0.28116129226886244 1102 74-75 0.24505945035856058 1102 76-77 0.21511058112101722 1102 78-79 0.2729872863045628 1102 80-81 0.3271927137258217 1102 82-83 0.3012339550009173 1102 84-85 0.3000139776054844 1102 86-87 0.26076428234060955 1102 88-89 0.2762280009149478 1102 90-91 0.23428976566718518 1102 92-93 0.2358011700437359 1102 94-95 0.22757532376093437 1102 96-97 0.11733004674749381 1102 98-99 0.10558163602522086 1102 100-101 -0.0010059914130415848 1103 1 0.5679753400432404 1103 2 0.4565134167157723 1103 3 1.9785787136959883 1103 4 1.9829227475465387 1103 5 1.0103907588375733 1103 6 0.5889287072174625 1103 7 0.40002982237871976 1103 8 0.31637701408708097 1103 9 0.2806528732230902 1103 10-11 0.2797615622501013 1103 12-13 0.27241099076231023 1103 14-15 0.32927953774915864 1103 16-17 0.3257464974585744 1103 18-19 0.2448744227645392 1103 20-21 0.2904285093180121 1103 22-23 0.3038782009176515 1103 24-25 0.30105012528927233 1103 26-27 0.2643777174297739 1103 28-29 0.24526979186852316 1103 30-31 0.24161491457062567 1103 32-33 0.23925468820873874 1103 34-35 0.28224050226803854 1103 36-37 0.2529405625520269 1103 38-39 0.1613517491639982 1103 40-41 0.2361104143215016 1103 42-43 0.19659681864876433 1103 44-45 0.2858983514676794 1103 46-47 0.2942837314959519 1103 48-49 0.3810716505536291 1103 50-51 0.38076429483396623 1103 52-53 0.25134665546185175 1103 54-55 0.2529391958639806 1103 56-57 0.26011743794870057 1103 58-59 0.22608571003480193 1103 60-61 0.27190012147831766 1103 62-63 0.28653048262204805 1103 64-65 0.22783568365814233 1103 66-67 0.23601152916172197 1103 68-69 0.22744971240019396 1103 70-71 0.19036295301355466 1103 72-73 0.1878578068593626 1103 74-75 0.22924175085145038 1103 76-77 0.1763996026071979 1103 78-79 0.20224391535283814 1103 80-81 0.1908409992697102 1103 82-83 0.16020546572444516 1103 84-85 0.12848283757856294 1103 86-87 0.2137430755031886 1103 88-89 0.19607651148493233 1103 90-91 0.22709175896848421 1103 92-93 0.09478096280256665 1103 94-95 0.04795993927938724 1103 96-97 0.0756128108645413 1103 98-99 0.001688795699696044 1103 100-101 -0.05726459219740221 1104 1 0.48751532806666376 1104 2 0.3793632348629927 1104 3 1.8858537109211468 1104 4 1.9238124589140568 1104 5 0.9437371876329905 1104 6 0.5146141454850977 1104 7 0.3073776809464803 1104 8 0.26452316338985327 1104 9 0.24401900988046776 1104 10-11 0.22030992045247189 1104 12-13 0.21096943051814776 1104 14-15 0.20963065988956942 1104 16-17 0.21849618249920155 1104 18-19 0.21341043212209598 1104 20-21 0.20531711073303427 1104 22-23 0.20873921825091912 1104 24-25 0.19689378070469132 1104 26-27 0.18977126346282347 1104 28-29 0.19185714780913088 1104 30-31 0.23566401043472496 1104 32-33 0.2017003216909572 1104 34-35 0.17907837751984346 1104 36-37 0.1964556645920723 1104 38-39 0.14162837530084005 1104 40-41 0.12714075388854695 1104 42-43 0.11666268288547599 1104 44-45 0.24196454276398782 1104 46-47 0.25649498706575713 1104 48-49 0.2728615814447153 1104 50-51 0.26089031500647053 1104 52-53 0.20777062007352498 1104 54-55 0.19297512827706242 1104 56-57 0.17950727539703593 1104 58-59 0.21497467380701352 1104 60-61 0.21373135877841776 1104 62-63 0.2386381544132874 1104 64-65 0.22297058824437954 1104 66-67 0.24758413906734944 1104 68-69 0.17938240267687888 1104 70-71 0.17017263841847807 1104 72-73 0.18927916091775643 1104 74-75 0.1921283390244355 1104 76-77 0.15430706642374048 1104 78-79 0.23389201215756117 1104 80-81 0.28019883512638444 1104 82-83 0.24146706488834013 1104 84-85 0.15427021108945027 1104 86-87 0.17224661712512557 1104 88-89 0.19207048947599503 1104 90-91 0.2183465654317125 1104 92-93 0.16613150966017542 1104 94-95 0.14099110209013688 1104 96-97 0.10918943522357694 1104 98-99 0.009117698845216182 1104 100-101 -0.09021149267115547 1105 1 0.468635867124064 1105 2 0.39838805367609353 1105 3 1.8899042364802057 1105 4 1.8803839011553407 1105 5 0.9617280630057863 1105 6 0.5159740431763495 1105 7 0.34560683234473544 1105 8 0.25537250639768416 1105 9 0.19447230443618935 1105 10-11 0.15699410474523035 1105 12-13 0.17349355436874703 1105 14-15 0.18779818664928882 1105 16-17 0.16709780777855343 1105 18-19 0.12105361092865508 1105 20-21 0.11239580887394851 1105 22-23 0.23231747878343612 1105 24-25 0.18432185969361115 1105 26-27 0.176533972120815 1105 28-29 0.1727512067638557 1105 30-31 0.22085879151467225 1105 32-33 0.19937675835836188 1105 34-35 0.15520948643946753 1105 36-37 0.2127914624113174 1105 38-39 0.23265680978480674 1105 40-41 0.2150351943591957 1105 42-43 0.1531978916132033 1105 44-45 0.24382433160329242 1105 46-47 0.22681616533604654 1105 48-49 0.1878403168008873 1105 50-51 0.19455694728917905 1105 52-53 0.22657715043340687 1105 54-55 0.23845477544769977 1105 56-57 0.2072507563768866 1105 58-59 0.20248284353560564 1105 60-61 0.28783239320916465 1105 62-63 0.2578235387302712 1105 64-65 0.20255016925506197 1105 66-67 0.2302506887808491 1105 68-69 0.23579729764770718 1105 70-71 0.22460420530163816 1105 72-73 0.2382660883585217 1105 74-75 0.28511592771086924 1105 76-77 0.19269725123288595 1105 78-79 0.21852909132562104 1105 80-81 0.2753461072346326 1105 82-83 0.26922436550849227 1105 84-85 0.2306178781238124 1105 86-87 0.2565952844372106 1105 88-89 0.2993368702328638 1105 90-91 0.2880826814188211 1105 92-93 0.2741391835764091 1105 94-95 0.28003187923247097 1105 96-97 0.18522025974250766 1105 98-99 0.11032419354873824 1105 100-101 -7.469620270192934E-4 1106 1 0.4379643690245132 1106 2 0.3927565551755805 1106 3 1.870499247724787 1106 4 1.9250412672258292 1106 5 0.9397788197949666 1106 6 0.5049966701127886 1106 7 0.35015460977599133 1106 8 0.22337530372492864 1106 9 0.21350893896999423 1106 10-11 0.1846477183716857 1106 12-13 0.14159899016664923 1106 14-15 0.15444246685352425 1106 16-17 0.12672623295186014 1106 18-19 0.13903579760075502 1106 20-21 0.10911638369926635 1106 22-23 0.14481301834086935 1106 24-25 0.15184990653872177 1106 26-27 0.19614911852634265 1106 28-29 0.20318669798800926 1106 30-31 0.21493977913666384 1106 32-33 0.2129190463581594 1106 34-35 0.18841918873184937 1106 36-37 0.20305247500967738 1106 38-39 0.19772540402929195 1106 40-41 0.21584906635410306 1106 42-43 0.18518430199202385 1106 44-45 0.20765524952699366 1106 46-47 0.12725877922875384 1106 48-49 0.18266127485351547 1106 50-51 0.14689367577460644 1106 52-53 0.17540594403995868 1106 54-55 0.21702794847936246 1106 56-57 0.23703238480194244 1106 58-59 0.1866762588794728 1106 60-61 0.24187989295914036 1106 62-63 0.23412776388953915 1106 64-65 0.27291598585173915 1106 66-67 0.2351058220055009 1106 68-69 0.24268581858245142 1106 70-71 0.22368527350378997 1106 72-73 0.19740586339979416 1106 74-75 0.24538756329398836 1106 76-77 0.22474845219232975 1106 78-79 0.22861019346325406 1106 80-81 0.19533713915009088 1106 82-83 0.2208568777924711 1106 84-85 0.18028207186040035 1106 86-87 0.26889570634006077 1106 88-89 0.27979823817234717 1106 90-91 0.23737014015424762 1106 92-93 0.21642742611660637 1106 94-95 0.13112518524306793 1106 96-97 0.06346438537812205 1106 98-99 -0.005139303420460095 1106 100-101 -0.0738993111191455 1107 1 0.3975594941367504 1107 2 0.34385447776172384 1107 3 1.7995437634277351 1107 4 1.8440664361850594 1107 5 0.8690035635778983 1107 6 0.46320730653153674 1107 7 0.29885481559280436 1107 8 0.2139728382094077 1107 9 0.21084065096134452 1107 10-11 0.0672337378230381 1107 12-13 0.07259463319272186 1107 14-15 0.037359504525973364 1107 16-17 0.05059319860585987 1107 18-19 0.07028110511583918 1107 20-21 0.011748326366408435 1107 22-23 -0.009201745280336127 1107 24-25 0.007661758990764156 1107 26-27 0.010887413628871911 1107 28-29 0.03238029135982856 1107 30-31 -0.014827012068373335 1107 32-33 -0.052996918889256506 1107 34-35 -0.0019867028535074382 1107 36-37 -0.10937397865568244 1107 38-39 0.005585728975574966 1107 40-41 -0.0487291902907927 1107 42-43 -0.02268816440901844 1107 44-45 0.035116600141122944 1107 46-47 -0.028884032372126 1107 48-49 0.06377365558712 1107 50-51 0.035805578948064465 1107 52-53 0.035321359756316895 1107 54-55 0.07346871482165085 1107 56-57 -7.578649443402696E-4 1107 58-59 0.022436543519141594 1107 60-61 0.10259719442057502 1107 62-63 0.11921116001014553 1107 64-65 0.09898496030007209 1107 66-67 0.09838164304850494 1107 68-69 0.02060178701572113 1107 70-71 -0.061401336210408886 1107 72-73 -0.021349828637731605 1107 74-75 0.04091197038152217 1107 76-77 -0.01383228656005997 1107 78-79 0.02172816867397387 1107 80-81 0.0634724879194053 1107 82-83 0.0837053000824497 1107 84-85 -0.002846854600239368 1107 86-87 0.08976078045647284 1107 88-89 -0.0017617931261675324 1107 90-91 0.04525242081216874 1107 92-93 0.0277030803482603 1107 94-95 0.03229289867183738 1107 96-97 -0.0016260404356245317 1107 98-99 -0.13320491281327307 1107 100-101 -0.29445733617076897 1108 1 0.37409109805236795 1108 2 0.28370118459383775 1108 3 1.76868851022369 1108 4 1.8332788511001255 1108 5 0.8762082012960946 1108 6 0.4245337041529069 1108 7 0.2685997195564198 1108 8 0.13937157860942762 1108 9 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-0.9494915123914929 2316 82-83 -0.9479458256433659 2316 84-85 -0.8824806816274737 2316 86-87 -0.9355131899377191 2316 88-89 -0.9335297482153493 2316 90-91 -0.9234709947678041 2316 92-93 -0.9810690871652064 2316 94-95 -1.076600277747783 2316 96-97 -1.1654881489963138 2316 98-99 -1.274944100308037 2316 100-101 -1.103583104828786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23333.0 3 2424.0 4 6709.0 5 11293.0 6 19593.0 7 39140.0 8 93976.0 9 186120.0 10 280887.0 11 239995.0 12 161668.0 13 119387.0 14 102042.0 15 93597.0 16 91059.0 17 89942.0 18 93785.0 19 98611.0 20 106679.0 21 118171.0 22 133765.0 23 155754.0 24 185858.0 25 225773.0 26 280873.0 27 361220.0 28 392615.0 29 462097.0 30 577455.0 31 721868.0 32 909928.0 33 1169366.0 34 1559934.0 35 2224600.0 36 3526239.0 37 6716632.0 38 1.0760001E7 39 3800161.0 40 7554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.446581643725306 17.483271245569348 10.58107457747086 48.489072533234484 2 15.78596616558664 19.626081095799677 53.36315764539615 11.224795093217532 3 19.70306627162623 23.426718774028014 35.919611080861486 20.950603873484262 4 20.344674527077434 31.297052423417647 31.34088632220809 17.01738672729683 5 19.45242259883955 32.79528324455166 32.10139589086659 15.650898265742194 6 15.903891606699158 34.656586966681125 34.001337675915764 15.438183750703951 7 13.975838077810232 17.61593825566864 50.517542079547 17.890681586974132 8 13.81648860539931 22.419642278207554 43.60446653210182 20.15940258429132 9 17.019478007587473 21.321783749225176 41.51063023221178 20.14810801097557 10-11 20.452304922829544 32.809566744261645 29.521356287107775 17.21677204580103 12-13 18.256130051520735 26.790799827297867 36.71805895772803 18.23501116345336 14-15 18.354670570242344 27.545999591038523 35.91439045375914 18.18493938496 16-17 19.175748372380088 28.047983489064336 34.04475226715477 18.731515871400802 18-19 20.645982097312913 27.95767752148099 32.73886846909209 18.657471912114 20-21 19.73119302493629 28.783363318740907 32.589097435267014 18.896346221055794 22-23 19.869922089297447 29.55437140651103 33.16891840753764 17.406788096653887 24-25 19.295893310098343 29.290345465530216 32.956530063125314 18.457231161246124 26-27 19.549259831906813 28.915893350422213 32.43832356717372 19.096523250497256 28-29 19.779769427499044 29.85994219463379 30.540340450442354 19.819947927424813 30-31 19.560294627217132 29.051587592049565 31.455176665512006 19.93294111522129 32-33 19.814445866969148 29.947325737012704 30.790880487094885 19.44734790892327 34-35 18.897859720760497 30.654804642585304 30.183200179367457 20.26413545728674 36-37 19.30459798970995 29.700043003149645 30.621013062362312 20.374345944778092 38-39 20.344380408719882 28.823162087841258 31.278209274767477 19.554248228671383 40-41 21.019251431588508 29.429512763011843 30.298096030058996 19.25313977534065 42-43 20.29503043370816 29.62652619958932 30.49711209267959 19.581331274022933 44-45 19.983162000063263 29.391570125651374 30.703567838241668 19.92170003604369 46-47 20.103710450584213 29.204075100255217 30.664301746323343 20.027912702837227 48-49 20.575234507210098 29.929098180440484 29.69883856773063 19.79682874461879 50-51 20.215150225077615 29.443835260112287 30.22061109921666 20.120403415593437 52-53 20.178432893823366 29.73099318817577 30.596901781793534 19.493672136207326 54-55 20.02491478682556 29.785926711496575 30.364525037426958 19.824633464250912 56-57 20.461103057723022 28.87478694871131 30.705201754028614 19.95890823953705 58-59 20.328208249190848 28.976743478381145 29.83742703809234 20.857621234335667 60-61 21.092472524811136 29.34196836568681 29.49298581123429 20.07257329826776 62-63 19.43806669215785 31.01387761921745 30.230989824895758 19.317065863728942 64-65 19.577753172111336 30.683523624443527 30.076665509475266 19.66205769396987 66-67 19.57336985094497 30.02148903543551 30.40767533760768 19.997465776011833 68-69 20.751836853304642 30.67307776758639 28.775739338512203 19.79934604059677 70-71 20.08647116627774 31.20632654641402 28.800370385216173 19.90683190209207 72-73 19.60348665228146 31.241256024273195 29.534814543756934 19.62044277968841 74-75 20.09028196721239 30.02652243195087 30.21724598879631 19.665949612040432 76-77 20.384607825250065 30.296772245380282 29.444819112692 19.873800816677655 78-79 20.20280715751598 31.01482237005141 28.73938188006378 20.042988592368825 80-81 20.143878473988202 30.56717641831587 30.148965054571754 19.139980053124173 82-83 19.86031554147778 30.85183019186286 29.797623444777006 19.490230821882353 84-85 19.90692636606118 30.60918264649481 29.501231297565432 19.982659689878574 86-87 20.619660618440978 30.632743992519963 28.814833693929597 19.93276169510946 88-89 20.03518083233576 30.463581975429264 29.44699954551614 20.054237646718835 90-91 20.10807458646357 30.681382048567805 29.161525256897132 20.049018108071486 92-93 19.639420089207555 31.112985972829765 29.163996399520446 20.083597538442234 94-95 19.426720501727797 30.9637764139968 29.023994925403233 20.58550815887217 96-97 19.751453103549903 30.267177581744793 29.53623507847391 20.44513423623139 98-99 20.266382536359533 30.622435690322124 29.068812572475633 20.042369200842707 100-101 20.569772399981034 30.9359444198274 28.345577094811315 20.148706085380258 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1814.0 1 5680.0 2 7893.5 3 6805.0 4 8434.0 5 10998.0 6 13359.5 7 15699.5 8 18640.5 9 21562.5 10 24932.5 11 28594.5 12 32279.0 13 36624.5 14 41089.5 15 45580.5 16 50922.5 17 57843.0 18 67202.5 19 80285.0 20 96930.0 21 119005.5 22 148014.0 23 184976.5 24 232909.5 25 293007.5 26 365108.5 27 452901.5 28 564513.0 29 677961.5 30 817572.0 31 980216.0 32 1196583.5 33 1437358.0 34 1635644.0 35 1829545.0 36 1967779.5 37 1954655.0 38 1782599.0 39 1567401.5 40 1434473.0 41 1350323.0 42 1282380.5 43 1251535.5 44 1206065.0 45 1147323.5 46 1090825.5 47 1026028.0 48 959531.0 49 888565.5 50 812123.5 51 737288.5 52 659505.0 53 585513.5 54 515055.5 55 451886.5 56 390314.5 57 321045.0 58 259721.5 59 210259.5 60 163012.5 61 123088.0 62 92826.0 63 69479.0 64 52503.5 65 38789.0 66 27985.5 67 20341.5 68 14650.0 69 10629.0 70 9079.0 71 9664.0 72 8859.5 73 10808.5 74 10776.0 75 6205.0 76 4502.0 77 4591.5 78 4223.5 79 2759.5 80 1801.5 81 1182.0 82 875.5 83 647.5 84 385.0 85 220.0 86 142.5 87 82.5 88 52.5 89 44.0 90 33.5 91 29.5 92 22.5 93 16.5 94 8.5 95 2.5 96 3.0 97 2.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09114773224442176 2 0.03512023091275201 3 0.002470255687231218 4 0.0 5 0.0 6 0.0064591792045743494 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.9156094267391315E-6 18-19 0.0 20-21 8.243406436673045E-4 22-23 0.0 24-25 7.88379474648261E-5 26-27 7.607170369413045E-4 28-29 4.785601723303479E-4 30-31 0.0022959823296773915 32-33 0.0021051115094993917 34-35 0.002348540961320609 36-37 0.004395561351635392 38-39 0.00423788545670574 40-41 0.0018326364980858702 42-43 3.8312476224134793E-4 44-45 0.0025380286596132615 46-47 0.0016957074314364353 48-49 7.081584052980872E-4 50-51 0.0031244723390007395 52-53 0.005544935638359436 54-55 0.010229569464032523 56-57 0.011539385889456915 58-59 0.003053933122848001 60-61 8.492368376035655E-4 62-63 9.723346853995219E-4 64-65 0.002206079407129783 66-67 0.002686022701345479 68-69 8.478537157182176E-4 70-71 0.0019723318085060003 72-73 0.002572606706746957 74-75 3.8312476224134793E-4 76-77 9.557372227753481E-4 78-79 1.3416282287873917E-4 80-81 2.2683198919704352E-4 82-83 0.010846441824897655 84-85 6.403854329160437E-4 86-87 7.053921615273915E-5 88-89 8.45087471947522E-4 90-91 0.002240657454263479 92-93 0.001313965791080435 94-95 0.003925299910617131 96-97 1.7980584509521744E-4 98-99 6.638985049669567E-5 100-101 0.002240657454263479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.6150104E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.754097696000656 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33357349379811 35.221532549178825 2 12.049053784343942 11.26685275555166 3 4.460599683770878 6.256539401933199 4 2.298134714907204 4.297888599173681 5 1.4169273138851948 3.3123579030760095 6 0.8756765669872544 2.4564880657812336 7 0.6546284249062178 2.1424592932851034 8 0.46171910620016204 1.7269808159513982 9 0.3470342034094117 1.460274394505166 >10 1.911739603628253 15.926725860391475 >50 0.1193959068088959 3.818450132734853 >100 0.06300230116499207 5.471540907139546 >500 0.0053626746075482725 1.7193210415546785 >1k 0.002842042292399964 2.5581210690878637 >5k 1.9087956601150896E-4 0.5907173108965933 >10k+ 1.1929972382922714E-4 1.7737498997588321 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG 196914 0.5447121258627637 TruSeq Adapter, Index 5 (100% over 49bp) CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 64657 0.1788570234818688 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 44053 0.12186133683045558 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 38403 0.10623205952602516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.6226593428334258 0.0 0.0 8.298731312086959E-6 2.766243770695653E-6 2 0.6355389738297849 5.532487541391306E-6 0.0 1.6597462624173918E-5 2.766243770695653E-6 3 0.6372236162861384 5.532487541391306E-6 0.0 1.6597462624173918E-5 2.766243770695653E-6 4 0.6435002234018469 5.532487541391306E-6 0.0 1.6597462624173918E-5 2.766243770695653E-6 5 0.6454282953100218 5.532487541391306E-6 0.0 1.936370639486957E-5 5.532487541391306E-6 6 0.64888056753585 5.532487541391306E-6 0.0 3.042868147765218E-5 8.298731312086959E-6 7 0.6500257924569179 5.532487541391306E-6 0.0 3.872741278973914E-5 8.298731312086959E-6 8 0.6523079435677419 5.532487541391306E-6 0.0 3.872741278973914E-5 8.298731312086959E-6 9 0.655187603333036 8.298731312086959E-6 0.0 4.425990033113045E-5 8.298731312086959E-6 10-11 0.6648003004362035 8.298731312086959E-6 0.0 4.5643022216478274E-5 9.681853197434785E-6 12-13 0.6789537866889677 8.298731312086959E-6 0.0 5.3941753528565226E-5 1.1064975082782612E-5 14-15 0.6972372748913807 8.298731312086959E-6 0.0 5.532487541391305E-5 1.1064975082782612E-5 16-17 0.7194737254421177 8.298731312086959E-6 0.0 5.670799729926088E-5 1.1064975082782612E-5 18-19 0.7419217936413129 8.298731312086959E-6 0.0 6.224048484065219E-5 1.1064975082782612E-5 20-21 0.7638816198149804 8.298731312086959E-6 0.0 6.77729723820435E-5 1.1064975082782612E-5 22-23 0.7838870947646512 8.298731312086959E-6 0.0 7.468858180878263E-5 1.1064975082782612E-5 24-25 0.8003808232474241 8.298731312086959E-6 0.0 8.298731312086959E-5 1.1064975082782612E-5 26-27 0.8141401197628644 8.298731312086959E-6 0.0 1.1341599459852176E-4 1.6597462624173918E-5 28-29 0.8265716192683705 8.298731312086959E-6 0.0 1.4384467607617395E-4 2.0746828280217397E-5 30-31 0.8379671604817513 8.298731312086959E-6 0.0 1.770396013245218E-4 2.2129950165565224E-5 32-33 0.8475508120253263 9.681853197434785E-6 0.0 1.8533833263660875E-4 2.6279315821608703E-5 34-35 0.8573599124362132 1.2448096968130438E-5 0.0 1.881045764073044E-4 2.7662437706956526E-5 36-37 0.8670708111932403 1.3831218853478263E-5 0.0 1.9502018583404353E-4 2.7662437706956526E-5 38-39 0.8762699548526887 1.5214340738826091E-5 0.0 2.0608516091682612E-4 2.7662437706956526E-5 40-41 0.8848771223452081 1.7980584509521744E-5 0.0 2.102345265728696E-4 2.7662437706956526E-5 42-43 0.8938203884558673 1.936370639486957E-5 0.0 2.102345265728696E-4 2.7662437706956526E-5 44-45 0.9026239592561062 1.936370639486957E-5 0.0 2.1715013599960873E-4 2.7662437706956526E-5 46-47 0.9113431596213388 2.2129950165565224E-5 0.0 2.2268262354100004E-4 2.7662437706956526E-5 48-49 0.9207373234666213 2.2129950165565224E-5 0.0 2.254488673116957E-4 2.7662437706956526E-5 50-51 0.9302974619381454 2.2129950165565224E-5 0.0 2.30981354853087E-4 2.9045559592304353E-5 52-53 0.9393665921403711 2.351307205091305E-5 0.0 2.3513072050913047E-4 3.042868147765218E-5 54-55 0.9488091652516408 2.4896193936260877E-5 0.0 2.3789696427982613E-4 3.3194925248347835E-5 56-57 0.958460589767598 2.4896193936260877E-5 0.0 2.4896193936260877E-4 3.3194925248347835E-5 58-59 0.9681258455024085 2.4896193936260877E-5 0.0 2.7385813329886964E-4 3.596116901904349E-5 60-61 0.9778159974311553 2.4896193936260877E-5 0.0 2.8907247403769573E-4 4.011053467508697E-5 62-63 0.9886389261840021 2.4896193936260877E-5 0.0 2.9045559592304356E-4 4.425990033113045E-5 64-65 1.0001409677825546 2.4896193936260877E-5 0.0 3.02903692891174E-4 4.5643022216478274E-5 66-67 1.0121575307224564 2.7662437706956526E-5 0.0 3.139686679739566E-4 4.979238787252175E-5 68-69 1.0256913783705852 2.7662437706956526E-5 0.0 3.195011555153479E-4 4.979238787252175E-5 70-71 1.0407051664360356 2.7662437706956526E-5 0.0 3.236505211713914E-4 5.25586316432174E-5 72-73 1.0563787036407972 3.042868147765218E-5 0.0 3.3194925248347835E-4 5.3941753528565226E-5 74-75 1.0737493313988806 3.042868147765218E-5 0.0 3.388648619102175E-4 5.670799729926088E-5 76-77 1.0935832992347685 3.3194925248347835E-5 0.0 3.4716359322230445E-4 5.8091119184608705E-5 78-79 1.115763041788206 3.3194925248347835E-5 0.0 3.554623245343914E-4 5.8091119184608705E-5 80-81 1.1412719587196762 3.3194925248347835E-5 0.0 3.6791042150252184E-4 5.8091119184608705E-5 82-83 1.1703451807496874 3.596116901904349E-5 0.0 3.8865724978273924E-4 5.8091119184608705E-5 84-85 1.2066507471181827 3.596116901904349E-5 0.0 4.052547124069131E-4 5.8091119184608705E-5 86-87 1.251996398129311 4.425990033113045E-5 0.0 4.149365656043479E-4 5.8091119184608705E-5 88-89 1.3038994299988738 4.425990033113045E-5 0.0 4.2738466257247833E-4 5.947424106995653E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 19195 0.0 56.688747 1 GCGTGTT 22365 0.0 45.784233 2 CGGAAGA 66730 0.0 35.82528 5 AGATCGG 59605 0.0 35.50637 1 TCGGAAG 63410 0.0 35.318943 4 GATCGGA 60985 0.0 35.09239 2 ATCGGAA 61205 0.0 35.024197 3 GTTCGGA 2805 0.0 34.726303 2 GTTTTTT 360675 0.0 31.270588 1 CGTGTTT 34735 0.0 29.195795 3 CTGGAAT 95880 0.0 28.838717 1 GAAGAGC 84170 0.0 28.439745 7 AGAGCAC 85250 0.0 28.163023 9 TATGCCG 50665 0.0 27.512033 46-47 TGCCGTC 51035 0.0 27.201958 48-49 CCGTCTT 52520 0.0 26.37502 50-51 CGTATGC 53310 0.0 26.244318 44-45 CTCGTAT 52350 0.0 26.185 42-43 ATCTCGT 52960 0.0 25.426245 40-41 AGTTCGG 3720 0.0 25.31344 1 >>END_MODULE