FastQCFastQC Report
Fri 27 May 2016
SRR1233075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37709170
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1275780.3383208911784587No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA626450.16612670074679448No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT522980.1386877515469049No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA453750.12032882187542182No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT428820.11371769784378706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412620.1094216605669125No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA410530.10886741872069845No Hit
CTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTTCAGT390170.1034682015011203No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT381570.1011875891195696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT262600.068.4089661
GCGTGTT324350.054.9912832
CGTGTTT500250.035.919673
CTGGAAT1477700.034.3693541
GTTTTTT4242600.033.167851
CGGCGAG30600.031.3888741
CGGACGT14700.025.2303351
GTCGCCG57100.024.823444-45
CTGGCGA33950.023.949811
CCGTATC64300.023.48882548-49
GAATATG2461350.022.7317664
CTCGTAT29150.022.347441
GGAATAT2641100.022.035033
CGCCGTA68400.021.83782846-47
TGGAATA2690000.021.7200282
CCGAAAC43750.020.9600722
TATGGCG2202950.020.7673477
TGGCGAG2168050.020.7128149
ATGGCGA2229800.020.5413888
TGGTCGC66400.020.23478742-43
Produced by FastQC (version 0.11.5)