FastQCFastQC Report
Fri 27 May 2016
SRR1233075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37709170
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1354920.35930782883844964No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA671210.17799649263030715No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT522570.1385790246775519No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA446060.11828953010633754No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA429450.11388476596010998No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT396330.10510175641627753No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT380350.10086406038637287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT251400.070.9537351
GCGTGTT311800.056.5447772
CGTGTTT472750.037.3617173
TTCGACT42700.035.9960672
CTGGAAT1533200.034.0897371
TCGACTC47250.033.232463
CCGATAG26500.032.3271681
ATTCGAC51100.030.3625131
CGATAGT30050.028.6625292
GTTTTTT3630150.026.4069981
TGCCGTC97950.026.40133948-49
TATGCCG100300.026.04576946-47
CGTCTCA48400.025.36971
CGGCGAG31450.025.2718371
CTCGTAT110100.023.03733442-43
CCGTCTT113100.022.969850-51
GAATATG2603350.022.4623344
CGTATGC120500.021.64032744-45
GGAATAT2792300.021.5258583
CGCGTTT14950.021.3292031
Produced by FastQC (version 0.11.5)