##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37709170 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.590400796411057 31.0 31.0 34.0 30.0 34.0 2 32.04168073707271 33.0 31.0 34.0 30.0 34.0 3 30.483728573182596 31.0 31.0 34.0 25.0 34.0 4 33.65357818801103 37.0 35.0 37.0 26.0 37.0 5 34.87644045201738 37.0 35.0 37.0 32.0 37.0 6 35.40206037417424 37.0 35.0 37.0 33.0 37.0 7 35.703813343014446 37.0 35.0 37.0 33.0 37.0 8 35.77848661214235 37.0 35.0 37.0 35.0 37.0 9 37.53731736869308 39.0 37.0 39.0 35.0 39.0 10-11 37.47394705319687 39.0 37.0 39.0 34.5 39.0 12-13 37.44982647456838 39.0 37.0 39.0 34.0 39.0 14-15 38.78245184128953 40.0 38.0 41.0 34.5 41.0 16-17 38.684403475335046 40.0 38.0 41.0 34.0 41.0 18-19 38.5680140268269 40.0 38.0 41.0 34.0 41.0 20-21 38.381219634375405 40.0 38.0 41.0 34.0 41.0 22-23 38.233022047422416 40.0 38.0 41.0 33.5 41.0 24-25 38.04856308160588 40.0 38.0 41.0 33.0 41.0 26-27 37.775371587335385 40.0 38.0 41.0 33.0 41.0 28-29 37.65570363919439 40.0 38.0 41.0 33.0 41.0 30-31 37.527417522050996 40.0 38.0 41.0 32.5 41.0 32-33 37.41703048621861 40.0 38.0 41.0 32.0 41.0 34-35 37.30581898249153 40.0 38.0 41.0 32.0 41.0 36-37 37.21471876734492 40.0 37.5 41.0 31.0 41.0 38-39 37.115082405685406 40.0 37.0 41.0 31.0 41.0 40-41 37.02711188021375 40.0 37.0 41.0 31.0 41.0 42-43 36.90888301174489 40.0 37.0 41.0 30.5 41.0 44-45 36.79779598702385 40.0 37.0 41.0 30.0 41.0 46-47 36.85046283171971 40.0 37.0 41.0 30.5 41.0 48-49 36.85322587317621 40.0 37.0 41.0 31.0 41.0 50-51 36.79151537941567 40.0 37.0 41.0 30.0 41.0 52-53 36.7078757766347 40.0 37.0 41.0 30.0 41.0 54-55 36.58219092332183 40.0 37.0 41.0 30.0 41.0 56-57 36.44196340041428 40.0 36.0 41.0 30.0 41.0 58-59 36.28532077210927 40.0 36.0 41.0 29.0 41.0 60-61 36.02568856593768 39.0 36.0 41.0 28.0 41.0 62-63 35.811988357208605 39.0 35.0 41.0 28.0 41.0 64-65 35.55976289056481 39.0 35.0 40.0 28.0 41.0 66-67 35.31785519278202 38.0 35.0 40.0 28.0 41.0 68-69 35.05205118542784 38.0 34.0 40.0 28.0 41.0 70-71 34.70664480284239 37.5 34.0 40.0 26.5 41.0 72-73 34.30676787635474 37.0 34.0 39.0 26.0 41.0 74-75 33.89148945733889 36.0 33.5 39.0 26.0 40.5 76-77 33.06103514078936 35.5 32.5 38.0 26.0 39.5 78-79 33.19779507212702 35.5 33.0 37.5 26.0 39.0 80-81 32.94425652964517 35.0 33.0 37.0 26.0 39.0 82-83 32.61298522348808 35.0 33.0 37.0 26.0 39.0 84-85 32.234664433080866 35.0 33.0 36.0 25.5 38.0 86-87 31.82606311674322 35.0 32.0 36.0 24.5 37.0 88-89 31.459919364441063 35.0 32.0 35.5 23.5 37.0 90-91 31.162067648266987 35.0 32.0 35.0 22.5 36.5 92-93 30.904432767944776 34.5 32.0 35.0 20.5 36.0 94-95 30.623936657847416 34.0 31.5 35.0 19.5 36.0 96-97 30.287044557066622 34.0 31.0 35.0 14.0 36.0 98-99 29.947825502391062 34.0 31.0 35.0 2.0 35.0 100-101 28.21948242297563 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9723407509592299 1101 2 0.7687033878125185 1101 3 2.3729260841485207 1101 4 2.5593225777799944 1101 5 1.2065177287627478 1101 6 0.6995529839938612 1101 7 0.46021052074871704 1101 8 0.37993412706432395 1101 9 0.41839982514679264 1101 10-11 0.3780306455219602 1101 12-13 0.40609423153224355 1101 14-15 0.4926252759219878 1101 16-17 0.4952671142910674 1101 18-19 0.4565599782380332 1101 20-21 0.5171285761338069 1101 22-23 0.5364266648028533 1101 24-25 0.4678809696471973 1101 26-27 0.505539748123347 1101 28-29 0.40275498702864354 1101 30-31 0.4446244516766171 1101 32-33 0.414194570719701 1101 34-35 0.4444637976762351 1101 36-37 0.47668397787389694 1101 38-39 0.44102927050020213 1101 40-41 0.4322104577214887 1101 42-43 0.44141960576268247 1101 44-45 0.4192125589648228 1101 46-47 0.43246492925046454 1101 48-49 0.3985929010784375 1101 50-51 0.4812200347782678 1101 52-53 0.5004491171215761 1101 54-55 0.518410436653646 1101 56-57 0.4956178885857767 1101 58-59 0.5545496314034537 1101 60-61 0.4720942389546181 1101 62-63 0.5040795336735613 1101 64-65 0.5520157687364815 1101 66-67 0.45757592443575135 1101 68-69 0.39067101922739766 1101 70-71 0.49196666617055484 1101 72-73 0.5215644040493501 1101 74-75 0.5487200314232936 1101 76-77 0.4843482471453697 1101 78-79 0.5143164048406916 1101 80-81 0.5040459186422623 1101 82-83 0.5094688390453896 1101 84-85 0.5119769929851472 1101 86-87 0.5923704316666338 1101 88-89 0.5287063981960358 1101 90-91 0.5453928826305336 1101 92-93 0.5631667968970753 1101 94-95 0.6429656114324693 1101 96-97 0.6336916992617141 1101 98-99 0.5867478472575876 1101 100-101 1.1539476938122384 1102 1 0.9229915764251864 1102 2 0.7104578635137351 1102 3 2.2970571687302446 1102 4 2.5237085535635018 1102 5 1.1961536503517607 1102 6 0.6809191617232742 1102 7 0.4109406760316858 1102 8 0.332557523773211 1102 9 0.2738052827152657 1102 10-11 0.3256893586645049 1102 12-13 0.2897655458924291 1102 14-15 0.36791429255158903 1102 16-17 0.34886719359774077 1102 18-19 0.3395843951607702 1102 20-21 0.4492396525737661 1102 22-23 0.4429380675875265 1102 24-25 0.32875135695228863 1102 26-27 0.3888285837724581 1102 28-29 0.38342340246017415 1102 30-31 0.3796124107896759 1102 32-33 0.41535292909971133 1102 34-35 0.3922758809985041 1102 36-37 0.37589603147416284 1102 38-39 0.38752113461234927 1102 40-41 0.37249051903325636 1102 42-43 0.38804470166588345 1102 44-45 0.35876580797108915 1102 46-47 0.3476087733170772 1102 48-49 0.328719958964804 1102 50-51 0.44134267193705057 1102 52-53 0.4289142764579239 1102 54-55 0.4194540282017769 1102 56-57 0.4184388829624268 1102 58-59 0.4194421843294478 1102 60-61 0.44049232016275397 1102 62-63 0.41624717365345987 1102 64-65 0.419656127536129 1102 66-67 0.4013882829733646 1102 68-69 0.38623739228510345 1102 70-71 0.36902027298246765 1102 72-73 0.42781745421898876 1102 74-75 0.4092168142325079 1102 76-77 0.33667649533919786 1102 78-79 0.31307800180868384 1102 80-81 0.33069980319265113 1102 82-83 0.41784112513401794 1102 84-85 0.375494705939893 1102 86-87 0.3573622352919834 1102 88-89 0.39798202901854296 1102 90-91 0.4219085935786957 1102 92-93 0.4649166110470375 1102 94-95 0.4503847560648566 1102 96-97 0.5020855427665651 1102 98-99 0.5282078333848332 1102 100-101 0.9332525158732743 1103 1 0.8709938424810453 1103 2 0.678256840615628 1103 3 2.2640630614847375 1103 4 2.4715342574381083 1103 5 1.1454625553116529 1103 6 0.6177545202928698 1103 7 0.39764232581029546 1103 8 0.28185188501457503 1103 9 0.2847820102780787 1103 10-11 0.3090224719414749 1103 12-13 0.24373232789685062 1103 14-15 0.3233988569534674 1103 16-17 0.322423798741994 1103 18-19 0.286025886326982 1103 20-21 0.397522909298722 1103 22-23 0.3463056895505545 1103 24-25 0.28467988420052137 1103 26-27 0.28675893194492375 1103 28-29 0.2954659881638264 1103 30-31 0.2775832594684715 1103 32-33 0.30352446542386957 1103 34-35 0.2879948511178654 1103 36-37 0.327715249029751 1103 38-39 0.3207130139653742 1103 40-41 0.3358784726011237 1103 42-43 0.2948302793231292 1103 44-45 0.29331081544530235 1103 46-47 0.26634378448610363 1103 48-49 0.28435089851397777 1103 50-51 0.3305928987765938 1103 52-53 0.3632038743866133 1103 54-55 0.429807093923678 1103 56-57 0.40405954714672276 1103 58-59 0.3105490652902674 1103 60-61 0.33390740979584876 1103 62-63 0.3444331862478123 1103 64-65 0.23842828473893007 1103 66-67 0.27418407644259446 1103 68-69 0.2784436996041322 1103 70-71 0.23408086218920232 1103 72-73 0.31773941901540326 1103 74-75 0.34553702381284523 1103 76-77 0.30465957564484114 1103 78-79 0.30792462708848234 1103 80-81 0.3321571440601687 1103 82-83 0.34194846949668545 1103 84-85 0.3275005221095668 1103 86-87 0.4297867657414365 1103 88-89 0.39292258370762667 1103 90-91 0.4045601622478081 1103 92-93 0.4131594200024935 1103 94-95 0.45119656506460615 1103 96-97 0.4397390575886284 1103 98-99 0.4487429380955561 1103 100-101 0.870776827203855 1104 1 0.8693622226168038 1104 2 0.6641905226694398 1104 3 2.2308271627206757 1104 4 2.478429203643138 1104 5 1.1158796661997172 1104 6 0.6058218706019289 1104 7 0.3904297936618235 1104 8 0.30830875341072783 1104 9 0.23733616182060757 1104 10-11 0.24641867089101055 1104 12-13 0.24731099094258013 1104 14-15 0.32740081940118415 1104 16-17 0.32244192137073924 1104 18-19 0.32334987587312014 1104 20-21 0.3478181734507828 1104 22-23 0.3275593121356337 1104 24-25 0.28953234400548666 1104 26-27 0.3322258311190964 1104 28-29 0.2794314086045233 1104 30-31 0.2928652186297569 1104 32-33 0.3096415994329291 1104 34-35 0.32039003945923383 1104 36-37 0.3367606361739348 1104 38-39 0.30939897798556615 1104 40-41 0.24184527241002485 1104 42-43 0.2644020311187134 1104 44-45 0.30422536474550554 1104 46-47 0.27668349629025357 1104 48-49 0.32142785345147473 1104 50-51 0.3820632476033765 1104 52-53 0.37902541623152075 1104 54-55 0.3455217666142687 1104 56-57 0.36414340928188693 1104 58-59 0.3679924135426518 1104 60-61 0.36142812509495315 1104 62-63 0.3503837129649412 1104 64-65 0.37839079085858884 1104 66-67 0.3546671682580538 1104 68-69 0.27712459263168654 1104 70-71 0.30150308576459395 1104 72-73 0.36566030041559117 1104 74-75 0.3995486570362985 1104 76-77 0.33894643923648715 1104 78-79 0.38998181615552596 1104 80-81 0.4313437391352153 1104 82-83 0.44784502033459006 1104 84-85 0.41241168821496643 1104 86-87 0.47104211939408813 1104 88-89 0.4463777089582166 1104 90-91 0.45731882884417274 1104 92-93 0.502272639643575 1104 94-95 0.48187518765046633 1104 96-97 0.5573000219597972 1104 98-99 0.6043410253657981 1104 100-101 1.0145172731207168 1105 1 0.7921094225464991 1105 2 0.5574684161370591 1105 3 2.197426255322309 1105 4 2.4228055575315324 1105 5 1.0801123605135743 1105 6 0.5573127022124496 1105 7 0.28423426227898574 1105 8 0.19153049835843916 1105 9 0.1581128972278094 1105 10-11 0.19990168581193757 1105 12-13 0.2039915487182995 1105 14-15 0.28031055513137204 1105 16-17 0.23195264898769352 1105 18-19 0.2496737089231047 1105 20-21 0.28669558780448057 1105 22-23 0.2561162769831071 1105 24-25 0.24244621251475706 1105 26-27 0.25139841422181775 1105 28-29 0.24172043700491486 1105 30-31 0.23910911258985834 1105 32-33 0.2035182059770122 1105 34-35 0.2177938006449338 1105 36-37 0.20020851854791033 1105 38-39 0.2318089476731089 1105 40-41 0.21406119181442307 1105 42-43 0.24660535968234853 1105 44-45 0.20646764213550028 1105 46-47 0.21250605236989628 1105 48-49 0.23037705789835883 1105 50-51 0.24370298839427562 1105 52-53 0.26005936359428716 1105 54-55 0.2690117053122165 1105 56-57 0.2663842223943007 1105 58-59 0.27617314699178763 1105 60-61 0.26207653011743304 1105 62-63 0.268193587469014 1105 64-65 0.25614351488633247 1105 66-67 0.29004676712786903 1105 68-69 0.2223184033304264 1105 70-71 0.20068015251142413 1105 72-73 0.24368164391575675 1105 74-75 0.2831469494671879 1105 76-77 0.23530038646792661 1105 78-79 0.25587308227199657 1105 80-81 0.3374646328851938 1105 82-83 0.22134953655561418 1105 84-85 0.2167151269510441 1105 86-87 0.3233701099904742 1105 88-89 0.270575808541615 1105 90-91 0.3038410178681268 1105 92-93 0.3444335948411421 1105 94-95 0.3304002292045638 1105 96-97 0.35970752850845145 1105 98-99 0.46598644681342805 1105 100-101 0.8931563244631064 1106 1 0.9458400622549981 1106 2 0.7060651343314568 1106 3 2.3309790659761482 1106 4 2.532516780965075 1106 5 1.2063431245293685 1106 6 0.6887280675995768 1106 7 0.4389117716285327 1106 8 0.3064292972444491 1106 9 0.2773508839407981 1106 10-11 0.30810956630426745 1106 12-13 0.344937484484106 1106 14-15 0.40423002265931984 1106 16-17 0.4478624513026901 1106 18-19 0.4454791512816172 1106 20-21 0.4746179837008384 1106 22-23 0.483122673787598 1106 24-25 0.43496435032206904 1106 26-27 0.45259978982384297 1106 28-29 0.4585604348301189 1106 30-31 0.4730656134519009 1106 32-33 0.5494500028956679 1106 34-35 0.5154114136353698 1106 36-37 0.5102031006601635 1106 38-39 0.5806525679566761 1106 40-41 0.564026732575087 1106 42-43 0.47170898481015655 1106 44-45 0.4908127502427462 1106 46-47 0.5164609193805347 1106 48-49 0.5597529488641442 1106 50-51 0.5878271171094056 1106 52-53 0.5512448393936609 1106 54-55 0.5810365745511845 1106 56-57 0.6198105437249453 1106 58-59 0.6582418868534745 1106 60-61 0.6284790497286821 1106 62-63 0.6428273964137929 1106 64-65 0.670108906667565 1106 66-67 0.7029241701505811 1106 68-69 0.6344430247654387 1106 70-71 0.6195693765772745 1106 72-73 0.6747627607913174 1106 74-75 0.6817618675580164 1106 76-77 0.43943408043384125 1106 78-79 0.6554232370094084 1106 80-81 0.6681105735337951 1106 82-83 0.6393791668372231 1106 84-85 0.6213578569344733 1106 86-87 0.5805700119077493 1106 88-89 0.7175739288638141 1106 90-91 0.7857674923518978 1106 92-93 0.7581896684509566 1106 94-95 0.7605777417467792 1106 96-97 0.9240808986478726 1106 98-99 1.0093254886729142 1106 100-101 1.4194296997641445 1107 1 0.4446179449298562 1107 2 0.33906530638771315 1107 3 -0.648326916803871 1107 4 1.2403939186084827 1107 5 0.5324822683292325 1107 6 0.2587047770780373 1107 7 0.19182574754459125 1107 8 0.14942432280537332 1107 9 0.10572486035481177 1107 10-11 0.12796873174639245 1107 12-13 0.06691551591367784 1107 14-15 0.1744573526111921 1107 16-17 0.13134935617681975 1107 18-19 0.11406101181378858 1107 20-21 0.21884394330030688 1107 22-23 0.21129119678244734 1107 24-25 0.12771211653340941 1107 26-27 0.1386460490042225 1107 28-29 0.14410255306071917 1107 30-31 0.09386770282686996 1107 32-33 0.12199821637729968 1107 34-35 0.1433736573023694 1107 36-37 0.13489009706238875 1107 38-39 0.1380763426699403 1107 40-41 0.12765715241227582 1107 42-43 0.1648359473856189 1107 44-45 0.13263512564433455 1107 46-47 0.12377428629527287 1107 48-49 0.11490857114105779 1107 50-51 0.14909448753245869 1107 52-53 0.15598976804238163 1107 54-55 0.07443016268846492 1107 56-57 0.16872663161934298 1107 58-59 0.18098827301696474 1107 60-61 0.19649644310332803 1107 62-63 0.1869853473354368 1107 64-65 0.1888674676367188 1107 66-67 0.1306888134365991 1107 68-69 0.08846867965086602 1107 70-71 0.10576707571512145 1107 72-73 0.0579473482108952 1107 74-75 0.12799642466671202 1107 76-77 0.09369762395441228 1107 78-79 0.19171150901655665 1107 80-81 0.22932678215899216 1107 82-83 0.20811480314305442 1107 84-85 0.185063621604165 1107 86-87 0.28999378822311783 1107 88-89 0.290215475552138 1107 90-91 0.25064499391650585 1107 92-93 0.1881362748006019 1107 94-95 0.2617351868304212 1107 96-97 0.30295665412687356 1107 98-99 0.4113553077540608 1107 100-101 0.7096461811705481 1108 1 0.3176802648535464 1108 2 0.25518565199496734 1108 3 -1.0938397797091959 1108 4 0.8020052678765026 1108 5 0.37581011072508375 1108 6 0.13643250740420854 1108 7 0.10356389167648672 1108 8 0.04699363119117095 1108 9 0.04717599494711777 1108 10-11 0.02422033005537827 1108 12-13 0.031187081020462415 1108 14-15 0.05424492746103482 1108 16-17 0.029853912418246864 1108 18-19 0.041806340911463735 1108 20-21 0.11845837611131316 1108 22-23 0.09769686234371733 1108 24-25 0.02267944833145208 1108 26-27 0.049248432694604105 1108 28-29 0.01449178468480028 1108 30-31 -0.015214802813638073 1108 32-33 -0.0280468988543916 1108 34-35 -0.04932436264412132 1108 36-37 -0.02954904202992026 1108 38-39 -0.06980377881742328 1108 40-41 -0.018473314718427503 1108 42-43 0.02780789891239266 1108 44-45 0.004005023057743529 1108 46-47 -0.03541615487244343 1108 48-49 -0.11501613665515009 1108 50-51 -0.04562195035726546 1108 52-53 -0.11221099889329622 1108 54-55 -0.0875748144227515 1108 56-57 -0.028937697161836695 1108 58-59 -0.08564483054350802 1108 60-61 -0.06261230857320044 1108 62-63 0.023431979955475413 1108 64-65 0.06282016404046686 1108 66-67 0.06446785137492128 1108 68-69 -0.03004437116385361 1108 70-71 -0.022443916602369995 1108 72-73 0.006965761991772013 1108 74-75 -0.08683731322593502 1108 76-77 0.001652278841724808 1108 78-79 0.02717123109704289 1108 80-81 0.08785404342366832 1108 82-83 0.08462701097041503 1108 84-85 0.05866189076854056 1108 86-87 0.1054082869618469 1108 88-89 0.0722711369650817 1108 90-91 0.10447174027997264 1108 92-93 0.021072774268201755 1108 94-95 0.09208144345697278 1108 96-97 0.12152393496991465 1108 98-99 0.2006034478073211 1108 100-101 0.5354308130543828 1109 1 0.2944832114676572 1109 2 0.21247535400164352 1109 3 -0.7659434047178628 1109 4 1.1314544619544122 1109 5 0.4121643715478882 1109 6 0.13665786609155361 1109 7 0.0902103458035981 1109 8 0.061765203193068885 1109 9 0.007331639786528399 1109 10-11 0.003110866828848202 1109 12-13 0.004424653879034679 1109 14-15 0.0013296325576774848 1109 16-17 -0.05304270232645081 1109 18-19 -0.04845750892150136 1109 20-21 0.06674768806509945 1109 22-23 0.061413658686298334 1109 24-25 -0.01589772253873889 1109 26-27 -0.04778563734455332 1109 28-29 -0.11980860775229019 1109 30-31 -0.16380914652532397 1109 32-33 -0.10454877693818077 1109 34-35 -0.05508434317635391 1109 36-37 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1379.0 5 3614.0 6 10888.0 7 33410.0 8 100703.0 9 209574.0 10 307587.0 11 246829.0 12 159291.0 13 104568.0 14 80001.0 15 72901.0 16 72414.0 17 74599.0 18 79172.0 19 85025.0 20 94788.0 21 106053.0 22 122948.0 23 145590.0 24 176917.0 25 220147.0 26 278170.0 27 355162.0 28 441916.0 29 557314.0 30 701990.0 31 879201.0 32 1105054.0 33 1406788.0 34 1844994.0 35 2539421.0 36 3843111.0 37 7033360.0 38 1.0890196E7 39 3313855.0 40 5189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.149329718061818 18.002872445536667 9.804228814202382 44.04356902219914 2 17.574478210046003 18.67999257232082 50.42492753318787 13.320601684445307 3 21.452874075370715 24.75587930289118 31.321096002312732 22.47015061942537 4 22.707349591445848 32.362657086887914 27.720265402333943 17.209727919332295 5 18.900633480236564 34.55859005995087 32.41360205301559 14.12717440679698 6 14.789392322825828 38.776748853249785 31.985960999765762 14.447897824158629 7 12.784253803517817 19.91781839801831 49.9042195837246 17.393708214739277 8 13.640514062923389 23.70219722222384 44.07551343363328 18.581775281219485 9 17.304135837516448 21.850594961384726 40.639146923679306 20.206122277419524 10-11 20.632035921236135 33.63710073703558 28.120239718880054 17.610623622848234 12-13 18.02841855177401 29.40112577391653 35.120672239670085 17.449783434639375 14-15 18.01171041420429 29.16421522934607 35.60134577345511 17.222728582994534 16-17 18.791328475275375 29.09985290050139 34.48051760354311 17.628301020680116 18-19 19.327633039921057 30.31940639372333 32.07268682922483 18.280273737130784 20-21 19.323061613914565 30.30448759745749 32.27745599806879 18.094994790559156 22-23 19.64050473731554 30.285164437674496 33.85951462139663 16.214816203613346 24-25 18.231286531342192 30.927396958777386 33.638718153330636 17.202598356549785 26-27 18.83410747410991 29.945338951037282 32.89685314578683 18.32370042906598 28-29 18.764080218239844 30.99297811092529 30.793163235733644 19.449778435101216 30-31 18.490030053577573 29.726602594603385 32.2501529436307 19.533214408188336 32-33 19.172367092672683 30.796286420517877 31.71755702923188 18.31378945757756 34-35 17.53833310582404 32.55321314168808 30.19842500808658 19.7100287444013 36-37 17.997415721393708 30.56882358546772 31.954498494059912 19.47926219907866 38-39 19.447426183074302 29.32338738826657 32.106673257459654 19.122513171199472 40-41 20.597391828035462 29.696483640451383 31.01478234604474 18.691342185468415 42-43 19.733129899173065 31.533958450955033 30.446284020571124 18.286627629300778 44-45 19.276672428240495 30.931979373428682 31.273991575960718 18.51735662237011 46-47 18.84111791325857 30.340086996004572 31.369171649662135 19.449623441074717 48-49 19.587584289692852 30.738697772120243 29.863448168341815 19.810269769845085 50-51 19.117182902726313 30.250396654182527 31.162739726172706 19.469680716918457 52-53 18.724835275100734 30.794540779939684 32.03368269186694 18.446941253092632 54-55 18.819691570777998 31.498636877427273 30.988656609582517 18.693014942212216 56-57 19.550150733252124 30.024021083787204 31.0964261751278 19.329402007832872 58-59 19.89684074790458 29.363000237014347 30.24963279350415 20.490526221576925 60-61 20.918902180429065 29.926761136513473 30.225740898606606 18.92859578445086 62-63 18.62554880875781 33.01851675805982 30.163686841185992 18.192247591996384 64-65 18.31481761809972 31.55946356310537 31.537260220509705 18.5884585982852 66-67 18.794238952825054 30.31197967483028 31.88764950693672 19.006131865407948 68-69 20.813141032071073 30.648714110136567 29.576345306387935 18.96179955140443 70-71 20.065885203507012 31.581630734498322 29.225263983945233 19.127220078049433 72-73 18.565781103680315 32.84688528122384 30.041336880226073 18.545996734869767 74-75 19.10725392171473 30.32014481348372 31.983907679985368 18.588693584816188 76-77 19.378302885586187 30.931279003475186 30.208719940813328 19.48169817012529 78-79 18.883512949237545 32.25565691315932 29.093896789560738 19.766933348042397 80-81 19.304091532751624 30.78758908741797 31.87618109537232 18.032138284458085 82-83 18.805959177604457 31.84916135494456 30.96374579529872 18.381133672152256 84-85 18.619359427996518 31.803613611882593 30.54637866187405 19.030648298246835 86-87 19.950061218531197 31.22772391967259 29.693021617818687 19.129193243977525 88-89 19.791436588807148 30.286146693024545 31.042378170499973 18.88003854766833 90-91 19.91662398694726 31.103930245926676 30.315899049162237 18.66354671796383 92-93 18.631032783626587 32.34875742926345 30.26250723870322 18.75770254840674 94-95 18.152156206648975 31.998445306197482 30.577238891763308 19.272159595390235 96-97 18.711961315811372 30.301657510210887 31.588744139575482 19.397637034402262 98-99 19.176998592119634 31.108549989299682 30.47444560567098 19.240005812909697 100-101 19.83922600365634 31.779230784467984 29.160448796603745 19.221094415271935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2119.0 1 2116.0 2 2242.0 3 2923.0 4 4205.5 5 5988.5 6 7983.0 7 10075.5 8 12637.0 9 15714.0 10 19129.5 11 22746.0 12 26713.0 13 31090.5 14 35831.5 15 41256.5 16 47191.5 17 53928.5 18 63368.0 19 75660.5 20 90291.0 21 109070.0 22 133042.0 23 163247.0 24 201497.0 25 250551.5 26 309749.0 27 388544.5 28 504564.5 29 633152.5 30 792302.0 31 1017496.5 32 1436113.0 33 1862452.0 34 2193558.5 35 2612883.0 36 2941636.0 37 2889653.5 38 2506313.5 39 2045249.5 40 1710278.5 41 1473893.5 42 1251624.0 43 1122034.0 44 1029196.5 45 947430.0 46 875464.5 47 804386.0 48 739179.5 49 675918.0 50 604645.5 51 528480.0 52 457821.5 53 395081.0 54 335251.0 55 278798.5 56 229905.5 57 182032.0 58 139545.0 59 104671.0 60 74943.5 61 52360.5 62 36620.5 63 25005.0 64 16555.0 65 11030.0 66 7440.0 67 5128.5 68 3484.0 69 2429.0 70 2381.0 71 3068.5 72 3047.5 73 4149.0 74 4350.5 75 2377.5 76 1539.5 77 1468.0 78 1273.0 79 692.0 80 400.0 81 219.5 82 125.0 83 79.0 84 52.0 85 29.0 86 16.5 87 10.0 88 6.5 89 9.5 90 6.5 91 3.5 92 4.5 93 3.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014343991130008959 2 0.004147532284587542 3 0.012914630579246373 4 0.17647431645936518 5 0.002988662969776317 6 5.754568451122102E-4 7 0.0 8 3.1822498347218995E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.05953406028295E-4 22-23 1.3259374311341246E-4 24-25 7.02746838501086E-5 26-27 1.352456179756807E-4 28-29 4.1104060365157864E-5 30-31 4.1104060365157864E-5 32-33 0.0 34-35 2.320390504484718E-4 36-37 1.3259374311341247E-6 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0026850232980466025 46-47 0.008447547373755507 48-49 4.1236654108271276E-4 50-51 0.0 52-53 2.784468605381662E-5 54-55 4.2960372768745643E-4 56-57 1.458531174247537E-5 58-59 0.0012795296210444302 60-61 0.005132703795920197 62-63 0.00874720923319182 64-65 0.014286975820470194 66-67 0.013214292438682686 68-69 0.001576539605618474 70-71 6.576649658425258E-4 72-73 2.850765476938368E-4 74-75 6.642946529981965E-4 76-77 9.281562017938872E-6 78-79 0.0 80-81 2.6518748622682495E-6 82-83 1.0740093192186411E-4 84-85 7.690437100577923E-5 86-87 0.0 88-89 3.977812293402374E-5 90-91 7.955624586804749E-6 92-93 0.00338511826168542 94-95 0.020831802980548233 96-97 0.0033347326393023237 98-99 0.0 100-101 0.06125963525582769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.770917E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61072274925871 #Duplication Level Percentage of deduplicated Percentage of total 1 77.00779202927203 35.12381051779702 2 11.327078756780159 10.33272497469036 3 4.217385542923608 5.770740081750617 4 2.1699098337640743 3.9588462327481304 5 1.3105531864379931 2.9887639017390435 6 0.8260218706107247 2.260527271514989 7 0.5784489112488954 1.8468431040908748 8 0.4343395827832928 1.5848433831485975 9 0.30157986971748013 1.2379748241997395 >10 1.6449145153995248 13.39768979302461 >50 0.10912269712689565 3.428210995016687 >100 0.06108651380725688 5.354734371899987 >500 0.005609276077652528 1.7489278439253946 >1k 0.0050423017999082186 4.606719133972946 >5k 6.313069410078124E-4 1.957389852205708 >10k+ 4.8380530975517845E-4 4.401253718275387 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 135492 0.35930782883844964 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 67121 0.17799649263030715 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 52257 0.1385790246775519 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 44606 0.11828953010633754 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 42945 0.11388476596010998 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 39633 0.10510175641627753 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 38035 0.10086406038637287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.06302445797666721 0.0 0.0 1.0607499449072998E-5 0.0 2 0.06333472733555259 2.6518748622682495E-6 0.0 1.5911249173609498E-5 0.0 3 0.0634514098294924 2.6518748622682495E-6 0.0 1.8563124035877745E-5 0.0 4 0.06397117730249698 2.6518748622682495E-6 0.0 3.712624807175549E-5 0.0 5 0.06422045353955019 2.6518748622682495E-6 0.0 3.977812293402374E-5 0.0 6 0.06461823476889043 2.6518748622682495E-6 0.0 4.242999779629199E-5 0.0 7 0.06474287288741704 2.6518748622682495E-6 0.0 4.242999779629199E-5 0.0 8 0.06497358600043437 2.6518748622682495E-6 0.0 5.303749724536499E-5 0.0 9 0.06525203286097254 2.6518748622682495E-6 0.0 5.5689372107633235E-5 0.0 10-11 0.06620803374882026 2.6518748622682495E-6 0.0 6.497093412557211E-5 0.0 12-13 0.06769971335884614 2.6518748622682495E-6 0.0 8.353405816144985E-5 0.0 14-15 0.06954674420041597 2.6518748622682495E-6 0.0 9.546749504165699E-5 0.0 16-17 0.07152636878509921 2.6518748622682495E-6 0.0 1.0607499449072997E-4 0.0 18-19 0.07376322523142248 2.6518748622682495E-6 0.0 1.2066030623320535E-4 0.0 20-21 0.07603323011352411 2.6518748622682495E-6 0.0 1.246381185266077E-4 0.0 22-23 0.07821704906260202 2.6518748622682495E-6 0.0 1.352456179756807E-4 0.0 24-25 0.0801436361500399 2.6518748622682495E-6 0.0 1.4187530513135133E-4 0.0 26-27 0.08201055605307675 2.6518748622682495E-6 0.0 1.511568671492902E-4 0.0 28-29 0.083901342829874 2.6518748622682495E-6 0.0 1.723718660474362E-4 0.0 30-31 0.0856184318031927 2.6518748622682495E-6 0.0 1.8032749063424095E-4 0.0 32-33 0.08744424764586439 2.6518748622682495E-6 0.0 1.9491280237671633E-4 0.0 34-35 0.08937083473330228 2.6518748622682495E-6 0.0 1.9756467723898457E-4 0.0 36-37 0.09141012650238656 2.6518748622682495E-6 0.0 2.0552030182578932E-4 0.0 38-39 0.09359394545146446 2.6518748622682495E-6 0.0 2.1214998898145995E-4 0.0 40-41 0.09655078592289355 2.6518748622682495E-6 0.0 2.2143155099939882E-4 0.0 42-43 0.10004595699136311 3.977812293402374E-6 0.0 2.254093632928012E-4 0.0 44-45 0.10297097496444499 7.955624586804749E-6 0.0 2.3336498787960596E-4 0.0 46-47 0.10626725541824442 9.281562017938872E-6 0.0 2.413206124664107E-4 0.0 48-49 0.11007667365789275 1.0607499449072998E-5 0.0 2.5060217448434955E-4 0.0 50-51 0.11446685249237784 1.0607499449072998E-5 0.0 2.943581097117757E-4 0.0 52-53 0.1190307291303415 1.0607499449072998E-5 0.0 3.009877968674463E-4 0.0 54-55 0.1236197985794967 1.0607499449072998E-5 0.0 3.036396717297146E-4 0.0 56-57 0.12961701358051636 1.0607499449072998E-5 0.0 3.261806080589947E-4 0.0 58-59 0.13615918886573214 1.0607499449072998E-5 0.0 3.328102952146653E-4 2.6518748622682495E-6 60-61 0.14244280635187673 1.0607499449072998E-5 0.0 3.434177946637383E-4 2.6518748622682495E-6 62-63 0.15010672470383196 1.0607499449072998E-5 0.0 3.7921810530435965E-4 2.6518748622682495E-6 64-65 0.15804246022916973 1.0607499449072998E-5 0.0 4.189962282383834E-4 2.6518748622682495E-6 66-67 0.16718347287940838 1.0607499449072998E-5 0.0 4.601002886035413E-4 2.6518748622682495E-6 68-69 0.17757219265234425 1.0607499449072998E-5 0.0 4.733596629148825E-4 2.6518748622682495E-6 70-71 0.19117896257064262 1.0607499449072998E-5 0.0 4.8529309979508965E-4 2.6518748622682495E-6 72-73 0.2052590921518559 1.0607499449072998E-5 0.0 4.905968495196261E-4 2.6518748622682495E-6 74-75 0.22019710325101294 1.0607499449072998E-5 0.0 4.945746618130284E-4 2.6518748622682495E-6 76-77 0.2376172692212531 1.0607499449072998E-5 0.0 5.025302863998333E-4 2.6518748622682495E-6 78-79 0.25748909350166016 1.3259374311341247E-5 0.0 5.131377858489062E-4 2.6518748622682495E-6 80-81 0.2826408006328434 1.3259374311341247E-5 0.0 5.224193478668451E-4 2.6518748622682495E-6 82-83 0.30834807554767185 1.3259374311341247E-5 0.0 5.31700909884784E-4 2.6518748622682495E-6 84-85 0.3406585188695482 1.3259374311341247E-5 0.0 5.356787221781863E-4 2.6518748622682495E-6 86-87 0.3845377662780698 1.3259374311341247E-5 0.0 5.42308409333857E-4 2.6518748622682495E-6 88-89 0.4394938949862859 1.3259374311341247E-5 0.0 5.582196585074664E-4 2.6518748622682495E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 25140 0.0 70.953735 1 GCGTGTT 31180 0.0 56.544777 2 CGTGTTT 47275 0.0 37.361717 3 TTCGACT 4270 0.0 35.996067 2 CTGGAAT 153320 0.0 34.089737 1 TCGACTC 4725 0.0 33.23246 3 CCGATAG 2650 0.0 32.327168 1 ATTCGAC 5110 0.0 30.362513 1 CGATAGT 3005 0.0 28.662529 2 GTTTTTT 363015 0.0 26.406998 1 TGCCGTC 9795 0.0 26.401339 48-49 TATGCCG 10030 0.0 26.045769 46-47 CGTCTCA 4840 0.0 25.3697 1 CGGCGAG 3145 0.0 25.271837 1 CTCGTAT 11010 0.0 23.037334 42-43 CCGTCTT 11310 0.0 22.9698 50-51 GAATATG 260335 0.0 22.462334 4 CGTATGC 12050 0.0 21.640327 44-45 GGAATAT 279230 0.0 21.525858 3 CGCGTTT 1495 0.0 21.329203 1 >>END_MODULE