FastQCFastQC Report
Fri 27 May 2016
SRR1233074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94447685
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT7574850.037.0644721
CGCGTGT1201750.027.5618551
GCGTGTT1202100.026.6448252
CGTGTTT1314500.024.2003573
CTGGAAT1611800.017.7043671
CCCGTCT241500.016.3737771
CTTTATT2472100.016.2198721
CGCGTTT87800.015.4555751
GGAAAAC2253300.015.2413221
CTTTTAT1276500.015.1534041
CGGAGTC147050.015.0494921
CTCGCGT88000.014.9230151
ACCCGGT82450.014.8490756
CGGGAAT244100.014.685041
CGCCGTA149500.014.44766946-47
CCCGGTA82500.014.1388267
CCGGTAA84200.013.7921198
TCCGATC101150.013.6080531
TATGGCG1606400.013.376377
CGCGCGT57650.013.2457271
Produced by FastQC (version 0.11.5)