FastQCFastQC Report
Fri 27 May 2016
SRR1233074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94447685
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT44550.029.0400851
TTCGACT156450.026.5304622
CCGATAG52200.026.515461
GCGTGTT1165400.025.130892
GCGGGTT73650.024.5407311
CGCGTGT1234950.024.5262051
ATTCGAC165900.024.4556561
CGTGTTT1240950.023.8420033
GTTTTTT5779900.023.2408391
CGATAGT62700.022.3441282
CGCGTTT107800.022.0170171
CGGGTTT168800.019.9215761
TCGACTC239350.018.0558073
GTCGTTT126200.018.0531431
CGGGAAT228250.017.337611
GGTAAAC264950.016.9466861
CGCACTA70750.016.7811812
CTGGAAT1655550.016.6461141
GTAAACT441800.016.1025642
CGTCTCA251900.015.690961
Produced by FastQC (version 0.11.5)