##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94447685 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.40386100516916 31.0 30.0 33.0 27.0 34.0 2 30.02647384104756 31.0 30.0 34.0 25.0 34.0 3 26.62310278965546 31.0 23.0 31.0 16.0 34.0 4 29.107622479047528 33.0 19.0 35.0 16.0 37.0 5 32.70291580995342 35.0 32.0 37.0 22.0 37.0 6 34.354306217246084 35.0 33.0 37.0 31.0 37.0 7 35.069551942961866 35.0 35.0 37.0 32.0 37.0 8 35.292289112221226 37.0 35.0 37.0 33.0 37.0 9 37.140406405937846 39.0 37.0 39.0 33.0 39.0 10-11 37.176515644613204 39.0 37.0 39.0 33.0 39.0 12-13 37.13062865966487 39.0 37.0 39.0 33.0 39.0 14-15 38.51199601133686 40.0 38.0 41.0 34.0 41.0 16-17 38.46316892256279 40.0 38.0 41.0 34.0 41.0 18-19 38.31607371848235 40.0 38.0 41.0 34.0 41.0 20-21 38.216280054932 40.0 38.0 41.0 33.5 41.0 22-23 38.106786677725346 40.0 37.0 41.0 33.0 41.0 24-25 37.92784962913596 40.0 37.0 41.0 33.0 41.0 26-27 37.59355528407075 40.0 37.0 41.0 32.5 41.0 28-29 37.29173848993758 40.0 37.0 40.5 32.0 41.0 30-31 36.76967929918028 39.0 36.0 40.0 31.0 41.0 32-33 36.79802114260397 39.0 36.0 40.0 31.0 41.0 34-35 36.624523253269786 39.0 36.0 40.0 30.5 41.0 36-37 36.43529984879989 39.0 36.0 40.0 30.0 41.0 38-39 36.23832622790066 38.0 35.5 40.0 30.0 41.0 40-41 36.02178193674096 38.0 35.0 40.0 30.0 41.0 42-43 35.761425931191425 38.0 35.0 40.0 29.5 41.0 44-45 35.46048800984376 38.0 35.0 40.0 29.0 40.0 46-47 35.099181028100375 38.0 34.0 40.0 27.5 40.0 48-49 34.84685704578148 38.0 34.0 39.5 27.0 40.0 50-51 34.68530809410522 37.5 34.0 39.0 27.0 40.0 52-53 34.33482311398104 37.0 33.0 39.0 26.5 40.0 54-55 33.973179517316915 37.0 33.0 39.0 26.0 40.0 56-57 33.55215001299396 36.0 32.5 38.5 25.0 40.0 58-59 33.06862323306284 36.0 31.5 38.0 24.0 40.0 60-61 32.565004891332165 35.0 31.0 38.0 23.0 39.5 62-63 32.00169304837911 34.5 31.0 38.0 22.0 39.0 64-65 31.326685021448647 34.0 29.5 37.0 20.5 39.0 66-67 30.695792755534452 34.0 29.0 36.5 18.5 38.5 68-69 30.308420889299722 33.5 29.0 36.0 18.0 38.0 70-71 29.768412365003968 33.0 28.0 36.0 15.5 38.0 72-73 29.153752847409656 32.0 28.0 35.0 10.5 38.0 74-75 28.49507928648542 32.0 26.5 34.5 8.0 37.0 76-77 26.533110970374764 29.5 25.0 32.5 7.0 35.0 78-79 27.25719040122582 31.0 26.0 34.0 4.0 36.0 80-81 26.93148140158226 31.0 26.0 34.0 2.0 35.0 82-83 26.42638830162963 30.5 25.0 34.0 2.0 35.0 84-85 25.75353614013938 30.0 24.5 33.5 2.0 34.5 86-87 24.866795792824355 29.5 22.0 33.0 2.0 34.0 88-89 23.93714530430259 29.0 19.5 32.0 2.0 34.0 90-91 22.951508181486926 29.0 16.5 32.0 2.0 34.0 92-93 21.83597425389516 27.5 4.5 31.5 2.0 34.0 94-95 20.532237036831553 26.5 2.0 31.0 2.0 34.0 96-97 18.49496965965868 24.5 2.0 31.0 2.0 33.5 98-99 16.563470481039317 20.5 2.0 30.0 2.0 33.0 100-101 13.402799608058153 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4190724626697495 1101 2 2.444805321601944 1101 3 4.383423746701972 1101 4 2.604396465533778 1101 5 1.7827124384229975 1101 6 1.0834764137523365 1101 7 0.7430509447721647 1101 8 0.6771458333962102 1101 9 0.27931810575568505 1101 10-11 0.5080615467088947 1101 12-13 0.46456372049476613 1101 14-15 0.5931725437789623 1101 16-17 0.4532479953356443 1101 18-19 0.5730882439209637 1101 20-21 0.5636807558567938 1101 22-23 0.6201374240231203 1101 24-25 0.6631396807372312 1101 26-27 0.6908542715166632 1101 28-29 0.6662731000210513 1101 30-31 0.46162689038924043 1101 32-33 0.5539047637719463 1101 34-35 0.5402219729757505 1101 36-37 0.7168326078812157 1101 38-39 0.37063038661028713 1101 40-41 0.5216009414986189 1101 42-43 0.5658628304178066 1101 44-45 0.4517864816823973 1101 46-47 0.6274873496590629 1101 48-49 0.3859672188144856 1101 50-51 0.5420299666807296 1101 52-53 0.6914925616986878 1101 54-55 0.7355727087133701 1101 56-57 0.658040283081128 1101 58-59 0.44739557409855735 1101 60-61 0.5317244284449103 1101 62-63 0.40469823155801166 1101 64-65 0.3986003344394966 1101 66-67 0.45196207689177115 1101 68-69 0.726268100493229 1101 70-71 0.7894854218310066 1101 72-73 0.366672392643995 1101 74-75 0.7298559006436847 1101 76-77 0.8123196315030299 1101 78-79 0.43240980901132176 1101 80-81 0.40490277765241345 1101 82-83 0.42528472241537685 1101 84-85 0.48997086250660615 1101 86-87 0.7100558136713033 1101 88-89 0.3527384375940734 1101 90-91 0.5521779188378915 1101 92-93 0.729907476830153 1101 94-95 1.2811217283266032 1101 96-97 1.7319573389796012 1101 98-99 1.6566838056355628 1101 100-101 1.489205765378749 1102 1 1.5079726655528454 1102 2 2.533993424662075 1102 3 2.523688459173112 1102 4 4.067191656680762 1102 5 2.3664490521942554 1102 6 1.233573034828197 1102 7 0.6677434004786775 1102 8 0.5444698161898245 1102 9 0.5847961491340641 1102 10-11 0.5962942262388182 1102 12-13 0.50399587001246 1102 14-15 0.5370301147011816 1102 16-17 0.5055736280400964 1102 18-19 0.4775891726313546 1102 20-21 0.4938990945845916 1102 22-23 0.5103899843288318 1102 24-25 0.5567381495130164 1102 26-27 0.5563362221383983 1102 28-29 0.5928742387563375 1102 30-31 0.3727325286901646 1102 32-33 0.5147031261566326 1102 34-35 0.5258252918430983 1102 36-37 0.37522304728148015 1102 38-39 0.45896673384748965 1102 40-41 0.4828829723608621 1102 42-43 0.5256527019261199 1102 44-45 0.5772311800281784 1102 46-47 0.574111376242179 1102 48-49 0.6151407848069326 1102 50-51 0.6374981811734628 1102 52-53 0.6530652818854961 1102 54-55 0.6406505782438927 1102 56-57 0.6700715058633477 1102 58-59 0.698745066525511 1102 60-61 0.7094031297969181 1102 62-63 0.8053965428849317 1102 64-65 0.8109956483256617 1102 66-67 0.9027146219356581 1102 68-69 0.7385872788841716 1102 70-71 0.811643048669648 1102 72-73 0.8281392229160609 1102 74-75 0.6814758244687198 1102 76-77 0.7981469301133828 1102 78-79 1.0487057686633996 1102 80-81 0.6070982597980432 1102 82-83 0.882802286624667 1102 84-85 0.9283144078519463 1102 86-87 1.2457657903745343 1102 88-89 1.0917929055818654 1102 90-91 1.2772755507464808 1102 92-93 1.5274153972188351 1102 94-95 1.5330360686817741 1102 96-97 1.7551116118732715 1102 98-99 1.704215955714382 1102 100-101 1.4240886262535426 1103 1 1.0267692493191554 1103 2 2.010708366771606 1103 3 3.040560032005203 1103 4 0.5405715056722151 1103 5 0.728201476723747 1103 6 0.5413938063161083 1103 7 0.4172680355773082 1103 8 0.4214814889361165 1103 9 0.4465526592160458 1103 10-11 0.46309842251823596 1103 12-13 0.47317691340394674 1103 14-15 0.49909315355987616 1103 16-17 0.4580029949763684 1103 18-19 0.43309971960679405 1103 20-21 0.4713505736257986 1103 22-23 0.46215333019662097 1103 24-25 0.5016343722620817 1103 26-27 0.526185340207789 1103 28-29 0.5401426412872254 1103 30-31 0.3737695415048563 1103 32-33 0.5443879380524876 1103 34-35 0.4971859955427007 1103 36-37 0.5512096951355119 1103 38-39 0.4991619101003124 1103 40-41 0.5408176547143029 1103 42-43 0.560658715553167 1103 44-45 0.563018440267598 1103 46-47 0.5780326607408668 1103 48-49 0.5338742676230979 1103 50-51 0.6032113553242482 1103 52-53 0.5691501601361608 1103 54-55 0.4983737781151589 1103 56-57 0.6371824143612912 1103 58-59 0.7413315254249468 1103 60-61 0.7206809253228101 1103 62-63 0.8156786137259324 1103 64-65 0.7485191339331188 1103 66-67 0.8372967883443465 1103 68-69 0.887159388183612 1103 70-71 0.8370643870323384 1103 72-73 0.8204166217945357 1103 74-75 0.7994898088476177 1103 76-77 0.7704044909442835 1103 78-79 0.9702384767588619 1103 80-81 1.088189633167179 1103 82-83 0.7422932459087228 1103 84-85 0.7199025244095942 1103 86-87 0.7961643266671814 1103 88-89 0.9607096703126281 1103 90-91 1.1873222308594578 1103 92-93 1.2806462361661595 1103 94-95 1.282062971616643 1103 96-97 1.6937838213036294 1103 98-99 1.6792898797314493 1103 100-101 1.295137473600093 1104 1 0.4240221069510888 1104 2 0.08189369292256288 1104 3 -1.1520944243274265 1104 4 0.17373180802524146 1104 5 0.190529749779202 1104 6 0.1810641422085837 1104 7 0.16919134391311985 1104 8 0.25113863799867175 1104 9 0.28903523799019837 1104 10-11 0.3351566808867332 1104 12-13 0.33982081342362846 1104 14-15 0.38236547441945845 1104 16-17 0.31705264154515334 1104 18-19 0.3646662684526447 1104 20-21 0.3528225257643385 1104 22-23 0.3865225125770664 1104 24-25 0.38128382802310057 1104 26-27 0.39412337799183206 1104 28-29 0.42386882924007807 1104 30-31 0.20323612064800045 1104 32-33 0.3653781994993821 1104 34-35 0.3328712123031963 1104 36-37 0.3868257031960951 1104 38-39 0.4075723268498024 1104 40-41 0.4003465414482932 1104 42-43 0.37885773413658796 1104 44-45 0.36801259922879126 1104 46-47 0.36425750821615566 1104 48-49 0.35685295550880625 1104 50-51 0.28622101858162097 1104 52-53 0.3454271877316728 1104 54-55 0.3381884483362967 1104 56-57 0.4346609685297125 1104 58-59 0.5091115054231281 1104 60-61 0.4925348851037654 1104 62-63 0.5471174444951323 1104 64-65 0.5872556514175322 1104 66-67 0.6656281072749373 1104 68-69 0.651455197302738 1104 70-71 0.5799246089419121 1104 72-73 0.5236264911712674 1104 74-75 0.5839267307341025 1104 76-77 0.573351642976899 1104 78-79 0.729781816580104 1104 80-81 0.8345355557284471 1104 82-83 0.829698981791843 1104 84-85 0.7197665746568269 1104 86-87 0.7082451660023352 1104 88-89 0.6557759254568545 1104 90-91 0.8697141019194419 1104 92-93 0.9220347432505065 1104 94-95 0.8700232662538951 1104 96-97 0.8974635875514565 1104 98-99 0.6721559335480691 1104 100-101 0.7575258321223437 1105 1 0.6635292863380045 1105 2 1.9932415036357618 1105 3 -0.5279768319811424 1105 4 1.0975607463562547 1105 5 0.794895136665339 1105 6 0.3964832141345198 1105 7 0.26170679982998735 1105 8 0.2911877829478726 1105 9 0.28390860580734767 1105 10-11 0.32968495175218493 1105 12-13 0.32866391009950746 1105 14-15 0.3556385481687627 1105 16-17 0.3417085557851891 1105 18-19 0.33855877845007853 1105 20-21 0.3561173949900507 1105 22-23 0.32551529311466965 1105 24-25 0.3471478575246181 1105 26-27 0.33883041765106725 1105 28-29 0.33495729332079094 1105 30-31 0.19529313795965209 1105 32-33 0.3579206363709275 1105 34-35 0.3776896138903183 1105 36-37 0.3753911747749896 1105 38-39 0.39206962375563137 1105 40-41 0.36771590134456744 1105 42-43 0.3116681819963887 1105 44-45 0.24309070205261207 1105 46-47 0.21450005824384277 1105 48-49 0.14823430005416327 1105 50-51 0.3211965968696404 1105 52-53 0.3258308240390164 1105 54-55 0.3784895675514832 1105 56-57 0.4019888240438547 1105 58-59 0.46709283094480014 1105 60-61 0.3375095965496655 1105 62-63 0.4301196872565747 1105 64-65 0.2694266988634162 1105 66-67 0.38159707483110594 1105 68-69 0.3629641622618749 1105 70-71 0.33002303895279184 1105 72-73 0.30707187182123263 1105 74-75 0.3536756512525656 1105 76-77 0.24524536902687188 1105 78-79 0.36288044435168487 1105 80-81 0.5218772104597882 1105 82-83 0.42613981075056984 1105 84-85 0.3482846653179408 1105 86-87 0.45556719101697496 1105 88-89 0.34001500802446216 1105 90-91 0.5798898990348462 1105 92-93 0.6570617254076225 1105 94-95 0.7691562998874026 1105 96-97 1.0474032593140379 1105 98-99 0.9145705766259589 1105 100-101 0.9531170599000163 1106 1 0.7216003350514733 1106 2 1.9743149395784414 1106 3 1.4410201253335053 1106 4 0.7561297730001542 1106 5 0.8447080461932259 1106 6 0.5091908640672358 1106 7 0.3647970651853001 1106 8 0.35711053523965575 1106 9 0.33775039519461814 1106 10-11 0.3593252898685009 1106 12-13 0.29913454457270916 1106 14-15 0.261098324608362 1106 16-17 0.27679556209739786 1106 18-19 0.25257632595432966 1106 20-21 0.32729970782294515 1106 22-23 0.3089123421746294 1106 24-25 0.32624070658827264 1106 26-27 0.3581822826851777 1106 28-29 0.38363881107573405 1106 30-31 0.1829479764992996 1106 32-33 0.2677038589922134 1106 34-35 0.20322620047419093 1106 36-37 0.18801643701645077 1106 38-39 0.2942385470499147 1106 40-41 0.28199625507584614 1106 42-43 0.3190478229687983 1106 44-45 0.3188236032703742 1106 46-47 0.3376672625468302 1106 48-49 0.2578042214434859 1106 50-51 0.3280138944404172 1106 52-53 0.17220309938018374 1106 54-55 0.2175520137640774 1106 56-57 0.1881503596225258 1106 58-59 0.3166069296320515 1106 60-61 0.37572712480826453 1106 62-63 0.42329772425872747 1106 64-65 0.44269322124576505 1106 66-67 0.5105854235763374 1106 68-69 0.5264658782455385 1106 70-71 0.4877947489581125 1106 72-73 0.3867761192022776 1106 74-75 0.3058472177471856 1106 76-77 0.3188775155185013 1106 78-79 0.4470380692459237 1106 80-81 0.5445088006645733 1106 82-83 0.5483198187564682 1106 84-85 0.49057648163031686 1106 86-87 0.46434514197472154 1106 88-89 0.43757133242045043 1106 90-91 0.6199274369550594 1106 92-93 0.6005326843908065 1106 94-95 0.6229105290678767 1106 96-97 0.9263716124964958 1106 98-99 1.0134789210357162 1106 100-101 1.1076507794347012 1107 1 0.6312350354830265 1107 2 1.6420699165436723 1107 3 0.20107372205968588 1107 4 0.517715903445108 1107 5 0.45885046670463225 1107 6 0.2749102395864753 1107 7 0.28035248013637926 1107 8 0.3100395803021314 1107 9 0.2824804583007534 1107 10-11 0.24971137677747635 1107 12-13 0.20704389748922125 1107 14-15 0.1648216911818352 1107 16-17 0.15760900979061176 1107 18-19 0.10815999894314388 1107 20-21 0.1760463979938649 1107 22-23 0.17022338851730012 1107 24-25 0.22854717562627513 1107 26-27 0.2418616452356872 1107 28-29 0.1877727428635083 1107 30-31 0.10751903728414902 1107 32-33 0.23634820531062672 1107 34-35 0.2488248835082416 1107 36-37 0.1681513308406153 1107 38-39 0.1821440761384494 1107 40-41 0.15564961432217217 1107 42-43 0.11628586575723432 1107 44-45 0.15056534870988258 1107 46-47 0.17876218692870793 1107 48-49 0.17015418134635496 1107 50-51 0.1580675682803161 1107 52-53 0.15405204649223947 1107 54-55 0.05174746847785627 1107 56-57 0.07171594574769102 1107 58-59 0.2136420431613999 1107 60-61 0.22237035814271167 1107 62-63 0.2351775559366054 1107 64-65 0.22290342517947437 1107 66-67 0.1659154641996139 1107 68-69 0.19311838714055085 1107 70-71 0.2449349784220196 1107 72-73 0.25174585306229247 1107 74-75 0.20292975151286896 1107 76-77 0.12025905991313479 1107 78-79 0.22625002486041623 1107 80-81 0.29588798285472606 1107 82-83 0.22947159326263034 1107 84-85 0.24413113772376605 1107 86-87 0.3264090203741432 1107 88-89 0.18102334326486513 1107 90-91 0.3117857948037859 1107 92-93 0.34307103554152363 1107 94-95 0.15380773463255437 1107 96-97 0.5062112045171112 1107 98-99 0.7547783062788049 1107 100-101 0.8471657920777922 1108 1 0.787011146424156 1108 2 1.6580193541080597 1108 3 2.543423891134008 1108 4 2.066547798519778 1108 5 1.0709517827742374 1108 6 0.5483029697371009 1108 7 0.3872032867425119 1108 8 0.3388808536707799 1108 9 0.2195239602996466 1108 10-11 0.09482528063916362 1108 12-13 0.12519873578963825 1108 14-15 0.08512212144743359 1108 16-17 0.09015864171668397 1108 18-19 0.04126606255425003 1108 20-21 0.07452004794620137 1108 22-23 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56-57 -0.14076575037906025 2311 58-59 -0.14410344430681477 2311 60-61 -0.14928462646288665 2311 62-63 -0.18677649325143975 2311 64-65 -0.09280235019917527 2311 66-67 -0.1386051160404307 2311 68-69 -0.324524984223288 2311 70-71 -0.2657460118295525 2311 72-73 -0.26409154725549655 2311 74-75 -0.2369042837395341 2311 76-77 -0.0033258559970548163 2311 78-79 -0.0941172891451707 2311 80-81 -0.18293156870018734 2311 82-83 -0.11638152893777232 2311 84-85 -0.374427637509374 2311 86-87 -0.30952406866485305 2311 88-89 -0.39394268824642964 2311 90-91 -0.35642799452350715 2311 92-93 -0.45141815698115195 2311 94-95 -0.5357622134239719 2311 96-97 -0.918254721113474 2311 98-99 -0.8540319717918798 2311 100-101 -0.5195877547524574 2312 1 0.5668158376223751 2312 2 1.7602916463055145 2312 3 1.0146133664525507 2312 4 0.6046923210027018 2312 5 0.20890091962903057 2312 6 -0.15093545991314983 2312 7 -0.13505984787870062 2312 8 -0.09987553820329254 2312 9 -0.26054325322410676 2312 10-11 -0.2936354180045484 2312 12-13 -0.3136128798763309 2312 14-15 -0.39042944189426265 2312 16-17 -0.3717235909199843 2312 18-19 -0.29716944448378513 2312 20-21 -0.359469230472115 2312 22-23 -0.35323776559661724 2312 24-25 -0.3575495279667109 2312 26-27 -0.3788619848201762 2312 28-29 -0.3827526160059662 2312 30-31 -0.11569383857238336 2312 32-33 -0.26757044152873277 2312 34-35 -0.34888593990137196 2312 36-37 -0.3465879125432778 2312 38-39 -0.4217991981614091 2312 40-41 -0.38555468113867164 2312 42-43 -0.4241165915085148 2312 44-45 -0.4327722620818335 2312 46-47 -0.3843553175832639 2312 48-49 -0.45360874835979814 2312 50-51 -0.4941513870508274 2312 52-53 -0.4627722811862327 2312 54-55 -0.3519319115104693 2312 56-57 -0.4902529750868254 2312 58-59 -0.4407360518286296 2312 60-61 -0.6132834915952827 2312 62-63 -0.6156565709925026 2312 64-65 -0.65642085215406 2312 66-67 -0.8495733232504143 2312 68-69 -0.8322496862314068 2312 70-71 -0.7032202461498294 2312 72-73 -0.721863579647259 2312 74-75 -0.6097639986988526 2312 76-77 -0.44946469309577 2312 78-79 -0.639724514356363 2312 80-81 -0.674996098192711 2312 82-83 -0.5603883424052363 2312 84-85 -0.7358971875972742 2312 86-87 -0.6511021965606574 2312 88-89 -0.6305492258681404 2312 90-91 -0.7172281848253306 2312 92-93 -0.9469536565685921 2312 94-95 -1.3170323600121492 2312 96-97 -1.7277451596985003 2312 98-99 -1.6876434451130802 2312 100-101 -1.2662327650780227 2313 1 -0.5296893781061947 2313 2 -4.8884644372544805 2313 3 -2.6502581394779092 2313 4 -1.6941451227209647 2313 5 -0.9369380695539142 2313 6 -0.5045099901329664 2313 7 -0.3537974880149761 2313 8 -0.30570227995404764 2313 9 -0.3449214414014605 2313 10-11 -0.3083697385905211 2313 12-13 -0.29852829344136467 2313 14-15 -0.3124894722360594 2313 16-17 -0.3823624153992924 2313 18-19 -0.3555371072025437 2313 20-21 -0.37049451366040387 2313 22-23 -0.3836742603077994 2313 24-25 -0.3757059371865239 2313 26-27 -0.3922908910768328 2313 28-29 -0.36898682933510685 2313 30-31 -0.27474433645528507 2313 32-33 -0.3631110788658489 2313 34-35 -0.342823969760758 2313 36-37 -0.42809338182614454 2313 38-39 -0.34246535491377017 2313 40-41 -0.3939750025104729 2313 42-43 -0.3609714944789886 2313 44-45 -0.3967608837670511 2313 46-47 -0.31549410194128313 2313 48-49 -0.3996104706845287 2313 50-51 -0.447097286059396 2313 52-53 -0.4511352935462867 2313 54-55 -0.40197185072621266 2313 56-57 -0.4722195391951516 2313 58-59 -0.42363818431277167 2313 60-61 -0.4167790006895302 2313 62-63 -0.45447744026275316 2313 64-65 -0.431013195492838 2313 66-67 -0.4467935879190428 2313 68-69 -0.44387914135435835 2313 70-71 -0.4908084698311832 2313 72-73 -0.5899440614187021 2313 74-75 -0.5588833142986935 2313 76-77 -0.35013259485138093 2313 78-79 -0.3667347869205635 2313 80-81 -0.4873451155100419 2313 82-83 -0.41820540540063433 2313 84-85 -0.5931581053970625 2313 86-87 -0.6527961845862436 2313 88-89 -0.6081777990390727 2313 90-91 -0.5462379073051551 2313 92-93 -0.7488824131778706 2313 94-95 -0.8921745152891951 2313 96-97 -1.3447559430844471 2313 98-99 -1.315693296135377 2313 100-101 -1.0831409054902466 2314 1 -2.7128732137410374 2314 2 -6.371397845864983 2314 3 -3.13847494572331 2314 4 -3.5152396115854287 2314 5 -2.0667130725182297 2314 6 -1.0856772803256192 2314 7 -0.6249232959348134 2314 8 -0.5304845287871558 2314 9 -0.7482256040497148 2314 10-11 -0.7170019560581977 2314 12-13 -0.5689289373508473 2314 14-15 -0.7385489680465085 2314 16-17 -0.9277263553210844 2314 18-19 -0.8254932683396632 2314 20-21 -0.9809020196498395 2314 22-23 -0.7810300126343392 2314 24-25 -0.8028791892963483 2314 26-27 -0.732257628467508 2314 28-29 -0.7549091497276308 2314 30-31 -0.8842524079633023 2314 32-33 -0.9479985932485775 2314 34-35 -1.1376876326366627 2314 36-37 -1.0289363735464505 2314 38-39 -0.9446418069293685 2314 40-41 -0.9635469803567815 2314 42-43 -0.9037776162105615 2314 44-45 -0.787365261506153 2314 46-47 -0.7819145545537509 2314 48-49 -0.8863583141788851 2314 50-51 -1.0018441284949162 2314 52-53 -1.0788078631017868 2314 54-55 -1.1065820709055032 2314 56-57 -1.117933811470806 2314 58-59 -0.9267454958112751 2314 60-61 -0.9311474779157862 2314 62-63 -0.9449613124301699 2314 64-65 -1.0016299769086707 2314 66-67 -0.9321958375975683 2314 68-69 -1.014004848111675 2314 70-71 -0.97496640864318 2314 72-73 -0.8545948027332848 2314 74-75 -0.9287687927865313 2314 76-77 -0.7815242004503347 2314 78-79 -0.8962120270456175 2314 80-81 -0.9173397857030139 2314 82-83 -0.9573163927630404 2314 84-85 -1.1030438256777622 2314 86-87 -1.226026848199247 2314 88-89 -1.0679912760470565 2314 90-91 -1.1151014316747698 2314 92-93 -1.272860596072185 2314 94-95 -1.6079178998407855 2314 96-97 -1.931975222581066 2314 98-99 -1.7903139941164845 2314 100-101 -1.4251317447024192 2315 1 -6.3455342460695014 2315 2 -5.3228945688612725 2315 3 -3.01420531734329 2315 4 -2.9322788374027944 2315 5 -1.7364692696868111 2315 6 -1.3119081504765262 2315 7 -0.9164902756789601 2315 8 -0.7912899710577577 2315 9 -0.6916855670358188 2315 10-11 -0.7875251796531231 2315 12-13 -0.8620837415020546 2315 14-15 -1.01411686316397 2315 16-17 -1.0164074159228704 2315 18-19 -1.0366508193480897 2315 20-21 -1.1908655184622248 2315 22-23 -0.9813668476307029 2315 24-25 -0.9116609584934849 2315 26-27 -0.8005762482749645 2315 28-29 -0.833642036931586 2315 30-31 -0.7533248794934195 2315 32-33 -0.8073814398902854 2315 34-35 -0.9330929942019637 2315 36-37 -0.9390584807047233 2315 38-39 -0.8782410825835356 2315 40-41 -0.9496956547027509 2315 42-43 -0.8176816225696584 2315 44-45 -0.7603672921680555 2315 46-47 -0.9027089723163897 2315 48-49 -0.9111665816179837 2315 50-51 -1.0025216551345082 2315 52-53 -0.8715600043746576 2315 54-55 -0.8961539115797521 2315 56-57 -0.9484339928642527 2315 58-59 -0.9015229748678522 2315 60-61 -0.8725689745178258 2315 62-63 -0.9112131308960052 2315 64-65 -0.9289175327309707 2315 66-67 -0.9096088123557422 2315 68-69 -0.9734921227480697 2315 70-71 -0.9924487863360731 2315 72-73 -1.0062256521428417 2315 74-75 -1.0189475352341404 2315 76-77 -0.7488030567957153 2315 78-79 -0.9631477649642086 2315 80-81 -1.1373371458847252 2315 82-83 -1.088977731285894 2315 84-85 -1.423372346395059 2315 86-87 -1.4224672210739975 2315 88-89 -1.5034558400235234 2315 90-91 -1.5156767256207075 2315 92-93 -1.7034416942089052 2315 94-95 -1.8532345798569203 2315 96-97 -2.277724335528138 2315 98-99 -2.304409823182656 2315 100-101 -1.8617337720108793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14347.0 3 1398.0 4 4484.0 5 11308.0 6 32960.0 7 91061.0 8 221893.0 9 408295.0 10 530590.0 11 458108.0 12 345504.0 13 311257.0 14 320091.0 15 364288.0 16 432970.0 17 523405.0 18 624201.0 19 731507.0 20 840247.0 21 956182.0 22 1087961.0 23 1246835.0 24 1443190.0 25 1700663.0 26 2033817.0 27 2452684.0 28 2976181.0 29 3648857.0 30 4529085.0 31 5759764.0 32 7550476.0 33 1.0236049E7 34 1.4020501E7 35 1.6578872E7 36 1.040355E7 37 1534221.0 38 20830.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.771791447971445 16.712115372903863 14.726845260508597 41.7892479186161 2 19.832871499179678 21.939393220702023 43.06067004183321 15.167065238285089 3 23.422568733107692 24.647517135697615 31.43276740735627 20.497146723838423 4 23.9843030562369 31.250089401344244 26.59360470296334 18.172002839455516 5 21.62451755475204 34.53422747957521 28.040903955867062 15.800351009805691 6 17.325176366154448 35.804199965303546 29.210759374356293 17.659864294185716 7 15.218319308459382 17.22896922708628 47.911140634808675 19.641570829645666 8 17.286227040720632 22.097762338374466 37.11895153663152 23.497059084273378 9 18.902658122324546 21.70533772214745 36.759541538789435 22.632462616738568 10-11 21.928968931318963 33.152997873902365 25.83990491667424 19.078128278104433 12-13 19.9033771976518 26.67629280696504 33.17787090281779 20.242459092565372 14-15 20.673311897480602 27.303698338397602 32.160337757352124 19.862652006769675 16-17 21.575188151258327 27.536935464761758 30.453810638087898 20.43406574589201 18-19 22.105433817673774 27.895324273961826 29.390781256311367 20.60846065205304 20-21 21.36755390034176 28.62164064688298 29.565010513492208 20.445794939283054 22-23 21.514649617933994 28.791267356103013 30.169053375950927 19.52502965001207 24-25 21.58412473787491 28.815307656530305 29.939454471485423 19.661113134109367 26-27 21.74859958134805 28.68320797136455 29.34024956584499 20.227942881442413 28-29 21.880746984042393 28.76323714743955 28.46413440378231 20.891881464735746 30-31 21.391062406868087 28.647352331410183 28.856053186774112 21.10553207494762 32-33 21.63769020883118 28.639705208430133 28.67484611482923 21.04775846790946 34-35 21.205776800947415 29.225423266581394 28.113236850006825 21.45556308246437 36-37 21.405108244585396 28.526145522526374 28.616537146719732 21.4522090861685 38-39 21.664153176358525 28.068969442786322 29.00920903490252 21.25766834595263 40-41 21.86004347274367 28.34037806220449 28.645194426946514 21.154384038105327 42-43 21.76028330269485 28.749439689736345 28.6031248075654 20.887152200003406 44-45 21.629910251373552 28.52553770905025 28.759560385201606 21.084991654374587 46-47 21.61999841499556 28.320195989981123 28.683586580232223 21.376219014791097 48-49 22.104689490271785 28.371436525945555 28.19021344991145 21.333660533871214 50-51 21.71186901124794 28.36270613819829 28.506108810589975 21.4193160399638 52-53 21.307082172260664 28.778899453193603 28.801594600090286 21.11242377445545 54-55 21.50369425083149 28.628399016094647 28.614311234620587 21.253595498453272 56-57 21.656388921284865 28.078245401590163 28.61114462469902 21.65422105242595 58-59 21.78970082750043 28.350297839486483 28.230244076390015 21.629757256623073 60-61 22.154751066688398 28.40524995398246 28.087925606646685 21.352073372682455 62-63 21.224175055217074 29.17693800541538 28.112595348419607 21.486291590947943 64-65 21.132173329605695 28.935324354429646 28.376004133928745 21.556498182035906 66-67 21.421143355710626 28.064153187026236 28.733617981213623 21.78108547604952 68-69 21.911691641780315 28.807435036655477 27.79122325761611 21.489650063948098 70-71 21.42334828005578 29.21451171619506 27.834031612315325 21.52810839143384 72-73 21.512501868097665 28.975471447500272 28.18904349005484 21.32298319434722 74-75 21.628311482700713 28.56006264208593 28.47257240873612 21.339053466477235 76-77 21.752825386879522 28.48505445104345 28.225111605435327 21.5370085566417 78-79 21.504771662746418 29.01950746595854 27.79153718802107 21.68418368327397 80-81 21.454190221814333 28.894250822558543 28.55079666590028 21.100762289726845 82-83 21.41038290139139 28.866935171571434 28.454296682867348 21.268385244169828 84-85 21.419551998548194 28.77900501213979 28.209509317247957 21.591933672064066 86-87 21.594353000817332 28.87933356968993 27.941185112160237 21.5851283173325 88-89 21.23592494617523 28.710172197444546 28.46294485672147 21.59095799965875 90-91 21.2742434794364 29.0439147335608 27.840537697152573 21.841304089850226 92-93 21.00088790551416 29.08476201386252 28.125240560105798 21.78910952051752 94-95 20.916787421938597 29.02419251024307 27.962404104566087 22.096615963252255 96-97 21.026758228782437 28.892355147154504 28.106883582231113 21.974003041831946 98-99 21.336860188791288 28.87598039062577 27.955844020951915 21.831315399631023 100-101 21.181521962901503 29.238708674816056 27.56818662901996 22.011582733262482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27945.0 1 23649.5 2 16832.5 3 13864.0 4 14078.5 5 16512.5 6 19255.0 7 21937.5 8 25852.5 9 29999.0 10 34571.0 11 40337.5 12 48009.5 13 56534.0 14 67930.0 15 80266.0 16 89763.5 17 101864.5 18 122834.5 19 144625.5 20 166058.0 21 200010.0 22 248260.5 23 312284.5 24 400052.0 25 513678.0 26 653379.0 27 833236.0 28 1056097.0 29 1284857.0 30 1515099.0 31 1755100.0 32 2071218.0 33 2483048.5 34 2944195.5 35 3418002.0 36 3823722.0 37 3955500.0 38 3772182.0 39 3536087.0 40 3403942.5 41 3359290.5 42 3370041.5 43 3434009.5 44 3465244.0 45 3463347.5 46 3489605.5 47 3389447.5 48 3212886.5 49 3056898.0 50 2893134.0 51 2752889.0 52 2566039.5 53 2315890.5 54 2087943.0 55 1915501.5 56 1725196.0 57 1499319.5 58 1293136.5 59 1135621.0 60 981450.0 61 814934.0 62 654455.5 63 525841.5 64 435463.5 65 342450.0 66 255192.0 67 187537.0 68 133149.0 69 95237.5 70 67727.5 71 49849.0 72 37307.0 73 31254.5 74 25700.5 75 16663.0 76 11215.5 77 9378.0 78 7565.5 79 4669.5 80 2589.0 81 1588.0 82 1060.0 83 612.0 84 323.0 85 159.0 86 76.0 87 37.0 88 22.5 89 15.5 90 9.5 91 5.0 92 2.0 93 3.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15395189410942153 2 0.0 3 1.2176052806376355E-4 4 0.0 5 0.0032081252176800307 6 0.0 7 1.5881808008316986E-5 8 7.856201028114136E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0034511168802072805 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.3727911382899435E-4 26-27 0.0053299347675911804 28-29 0.014731965108514836 30-31 0.16697338849544063 32-33 0.07463920370308706 34-35 0.01159742560127334 36-37 3.4728220178186474E-4 38-39 3.1763616016633968E-6 40-41 0.0 42-43 1.3764233607208053E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.1763616016633968E-6 52-53 1.9058169609980383E-4 54-55 6.273314163285209E-4 56-57 9.529084804990191E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.223453121164378E-5 92-93 1.434656656751301E-4 94-95 0.0019513448106218802 96-97 0.001039199637344208 98-99 0.0 100-101 0.09199060834577365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9.4447685E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.071051542334615 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39394303152241 28.32003799643742 2 10.541456727321542 7.815657713396537 3 4.126070536044389 4.588733205270292 4 2.1990774225665985 3.26088449910203 5 1.3584078285949275 2.5178803314676705 6 0.9459534284174875 2.104049298090769 7 0.6469134492544796 1.6787233274519597 8 0.4921018890725653 1.459418759511144 9 0.37051445829616003 1.2361824522609426 >10 2.5027480138338647 17.581300518681793 >50 0.2362587944803029 6.090926418945632 >100 0.16233290054867827 11.73578119009312 >500 0.015472458222293284 3.9478954598059315 >1k 0.008184032754857249 5.560522800739911 >5k 4.1156335220799366E-4 0.9878945544172516 >10k+ 1.534657174511397E-4 1.1141114743276994 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.029811212418811534 0.0 0.0 3.176361601663397E-6 0.0 2 0.029912855990064765 2.1175744011089315E-6 0.0 1.0587872005544656E-5 1.0587872005544657E-6 3 0.030249550319841084 2.1175744011089315E-6 0.0 1.1646659206099123E-5 2.1175744011089315E-6 4 0.03196796194634098 2.1175744011089315E-6 0.0 1.482302080776252E-5 2.1175744011089315E-6 5 0.03247723858980768 2.1175744011089315E-6 0.0 1.694059520887145E-5 2.1175744011089315E-6 6 0.033044748529304876 2.1175744011089315E-6 0.0 1.7999382409425918E-5 2.1175744011089315E-6 7 0.033360267115070105 2.1175744011089315E-6 0.0 2.223453121164378E-5 2.1175744011089315E-6 8 0.03603158722206902 2.1175744011089315E-6 0.0 3.282240321718844E-5 2.1175744011089315E-6 9 0.03659592079996455 2.1175744011089315E-6 1.0587872005544657E-6 3.917512642051523E-5 2.1175744011089315E-6 10-11 0.038781257581908965 3.176361601663397E-6 1.5881808008316986E-6 4.2351488022178626E-5 2.1175744011089315E-6 12-13 0.04169662813863569 4.764542402495096E-6 2.1175744011089315E-6 4.658663682439649E-5 2.1175744011089315E-6 14-15 0.045097452626816636 5.293936002772328E-6 2.1175744011089315E-6 5.7174508829941145E-5 2.1175744011089315E-6 16-17 0.04864597792947492 5.293936002772328E-6 2.1175744011089315E-6 6.77623808354858E-5 2.1175744011089315E-6 18-19 0.052195032625733495 5.293936002772328E-6 2.1175744011089315E-6 7.358571043853537E-5 2.1175744011089315E-6 20-21 0.055563564104297525 5.293936002772328E-6 2.1175744011089315E-6 8.04678272421394E-5 2.1175744011089315E-6 22-23 0.058697574217938746 5.293936002772328E-6 2.1175744011089315E-6 8.682055044546618E-5 2.1175744011089315E-6 24-25 0.061490654853001425 5.293936002772328E-6 3.176361601663397E-6 8.999691204712959E-5 2.1175744011089315E-6 26-27 0.06413021134398371 5.293936002772328E-6 3.176361601663397E-6 9.740842245101083E-5 2.1175744011089315E-6 28-29 0.06670465242213189 6.882116803604027E-6 3.176361601663397E-6 1.016435712532287E-4 2.1175744011089315E-6 30-31 0.06921927202344874 7.41151040388126E-6 3.176361601663397E-6 1.1170204965849613E-4 3.70575520194063E-6 32-33 0.07155760355587329 8.470297604435726E-6 4.235148802217863E-6 1.1434901765988229E-4 5.823329603049561E-6 34-35 0.07391075810910558 8.470297604435726E-6 4.235148802217863E-6 1.2652507046625864E-4 6.352723203326794E-6 36-37 0.07643702436962854 9.529084804990192E-6 4.235148802217863E-6 1.4452445287568459E-4 6.352723203326794E-6 38-39 0.07904111148939225 1.0587872005544656E-5 4.235148802217863E-6 1.5564171848150645E-4 6.352723203326794E-6 40-41 0.08176007702041611 1.0587872005544656E-5 4.235148802217863E-6 1.6993534568899175E-4 6.352723203326794E-6 42-43 0.08477603236119552 1.2705446406653587E-5 5.293936002772328E-6 1.7840564329342745E-4 6.882116803604027E-6 44-45 0.08794074730365281 1.482302080776252E-5 5.293936002772328E-6 1.9958138730451677E-4 7.41151040388126E-6 46-47 0.09150462502071915 1.5881808008316986E-5 7.41151040388126E-6 2.1916895051477442E-4 7.41151040388126E-6 48-49 0.09546131278919118 1.5881808008316986E-5 1.0587872005544656E-5 2.3240379052170523E-4 7.41151040388126E-6 50-51 0.09959270044575472 1.5881808008316986E-5 1.0587872005544656E-5 2.4775620492974496E-4 7.41151040388126E-6 52-53 0.10405125334728957 1.5881808008316986E-5 1.0587872005544656E-5 2.562265025341807E-4 7.41151040388126E-6 54-55 0.10872950459293947 1.5881808008316986E-5 1.0587872005544656E-5 2.68402555340557E-4 9.529084804990192E-6 56-57 0.11373862683876265 1.641120160859422E-5 1.1646659206099123E-5 2.7740224654527004E-4 1.1646659206099123E-5 58-59 0.11924167331364448 1.694059520887145E-5 1.2705446406653587E-5 2.906370865522008E-4 1.1646659206099123E-5 60-61 0.12544351934089226 1.7469988809148685E-5 1.3764233607208053E-5 3.033425329588544E-4 1.1646659206099123E-5 62-63 0.13263639018785903 1.9587563210257614E-5 1.482302080776252E-5 3.1710676656606247E-4 1.1646659206099123E-5 64-65 0.14061170477603552 2.3293318412198245E-5 1.5881808008316986E-5 3.2928281937243884E-4 1.2176052806376356E-5 66-67 0.14952192846230167 2.4881499213029944E-5 1.5881808008316986E-5 3.4622341458131025E-4 1.2705446406653589E-5 68-69 0.15999386326938558 2.5410892813307178E-5 1.694059520887145E-5 3.5575249938630046E-4 1.2705446406653589E-5 70-71 0.1725246097879477 2.6469680013861644E-5 1.694059520887145E-5 3.790458177984987E-4 1.2705446406653589E-5 72-73 0.18688599937626846 3.0704828816079505E-5 1.7469988809148685E-5 3.928100514057068E-4 1.2705446406653589E-5 74-75 0.2042379334125553 3.0704828816079505E-5 1.7999382409425918E-5 4.0339792341125144E-4 1.2705446406653589E-5 76-77 0.22492451773698846 3.5998764818851836E-5 1.7999382409425918E-5 4.1345640181651884E-4 1.2705446406653589E-5 78-79 0.2503788208255184 4.129270082162416E-5 1.8528776009703148E-5 4.2457366742234073E-4 1.3764233607208053E-5 80-81 0.2823065488582383 4.182209442190139E-5 1.905816960998038E-5 4.3357335862705365E-4 1.3764233607208053E-5 82-83 0.3224351131528528 4.288088162245586E-5 1.905816960998038E-5 4.4839637943481624E-4 1.3764233607208053E-5 84-85 0.3735782406948355 4.446906242328756E-5 1.905816960998038E-5 4.6216061304202424E-4 1.3764233607208053E-5 86-87 0.43927969224444197 4.5527849623842024E-5 1.905816960998038E-5 4.7963060185117294E-4 1.3764233607208053E-5 88-89 0.5196591107553351 4.5527849623842024E-5 2.0116956810534847E-5 5.039827074639256E-4 1.4293627207485286E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGTT 4455 0.0 29.040085 1 TTCGACT 15645 0.0 26.530462 2 CCGATAG 5220 0.0 26.51546 1 GCGTGTT 116540 0.0 25.13089 2 GCGGGTT 7365 0.0 24.540731 1 CGCGTGT 123495 0.0 24.526205 1 ATTCGAC 16590 0.0 24.455656 1 CGTGTTT 124095 0.0 23.842003 3 GTTTTTT 577990 0.0 23.240839 1 CGATAGT 6270 0.0 22.344128 2 CGCGTTT 10780 0.0 22.017017 1 CGGGTTT 16880 0.0 19.921576 1 TCGACTC 23935 0.0 18.055807 3 GTCGTTT 12620 0.0 18.053143 1 CGGGAAT 22825 0.0 17.33761 1 GGTAAAC 26495 0.0 16.946686 1 CGCACTA 7075 0.0 16.781181 2 CTGGAAT 165555 0.0 16.646114 1 GTAAACT 44180 0.0 16.102564 2 CGTCTCA 25190 0.0 15.69096 1 >>END_MODULE