FastQCFastQC Report
Fri 27 May 2016
SRR1233073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44097160
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1405290.31868038667342746No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA935930.2122426931802411No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA883300.20030768421367726No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG758410.17198613244027505No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT446600.10127636337578201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT137200.056.518681
AGACGGC72900.040.70889
CTGGAAT1865200.035.017131
GCGTGTT221500.033.5689242
AAGACGG90000.030.8641348
CCCGAAA91500.026.5127811
CGAGCTA86700.026.04893912-13
CCGAAAC96750.025.4681951
GTTTTTT3476100.025.0388661
CATACGA83850.024.7279814-15
ACGGCAT68550.024.34881610-11
CGGCGAG53050.023.987471
ACTCGTC98300.023.89985342-43
GAAGACG136100.023.3756057
TCGTCTA101500.023.0526844-45
CTAACGA103600.022.70624494-95
CTCTACG44200.021.700956
TCTACGC50000.021.4627137
CGAAACC112750.021.2922822
CCCGTAT60300.021.2614821
Produced by FastQC (version 0.11.5)