##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233073_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44097160
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.135614787891104	31.0	28.0	31.0	16.0	34.0
2	28.263671606062612	31.0	28.0	31.0	16.0	34.0
3	28.41834836982699	31.0	28.0	31.0	16.0	34.0
4	31.870134924788807	35.0	32.0	37.0	19.0	37.0
5	31.731082772677425	35.0	32.0	37.0	19.0	37.0
6	31.993343131394404	35.0	32.0	37.0	19.0	37.0
7	31.933361785656945	35.0	32.0	37.0	19.0	37.0
8	31.97576458438593	35.0	32.0	37.0	19.0	37.0
9	33.08620725234913	37.0	32.0	39.0	19.0	39.0
10-11	32.576028558301715	37.0	31.5	39.0	17.0	39.0
12-13	32.28875780208975	37.0	31.0	39.0	17.0	39.0
14-15	33.09725782794175	37.0	31.5	40.0	17.0	41.0
16-17	32.73994866109292	37.0	31.0	40.0	17.0	41.0
18-19	32.5994004489178	37.0	30.5	40.0	16.5	41.0
20-21	32.985477704233105	37.0	31.0	40.0	18.0	41.0
22-23	33.002671521249894	37.0	31.5	40.0	18.0	41.0
24-25	32.27163656344309	36.5	30.0	40.0	13.5	41.0
26-27	31.168067115886828	36.0	27.5	39.5	10.0	41.0
28-29	31.075862028302957	36.0	27.5	39.0	9.5	41.0
30-31	31.105372296084372	36.0	28.5	40.0	9.0	41.0
32-33	31.075238042540608	36.0	29.0	40.0	8.5	41.0
34-35	30.7980882442316	36.0	27.5	40.0	8.0	41.0
36-37	30.54803693707259	35.0	27.0	40.0	8.0	41.0
38-39	30.646455021139683	35.0	28.0	40.0	4.5	41.0
40-41	30.517373454435614	35.0	27.5	40.0	2.0	41.0
42-43	30.294448259252977	35.0	27.0	39.0	2.0	41.0
44-45	30.128032134042193	35.0	27.0	39.0	2.0	41.0
46-47	29.965522416863127	35.0	27.0	39.0	2.0	41.0
48-49	29.804577233545196	35.0	26.5	39.0	2.0	41.0
50-51	29.629246554653406	35.0	26.0	39.0	2.0	40.5
52-53	29.504792644242848	35.0	26.0	39.0	2.0	40.0
54-55	29.345738841231498	35.0	26.0	39.0	2.0	40.0
56-57	29.133861409669013	34.0	25.0	39.0	2.0	40.0
58-59	28.847846278535854	34.0	24.5	39.0	2.0	40.0
60-61	28.57009576126898	34.0	24.0	39.0	2.0	40.0
62-63	28.345256157085853	34.0	23.0	39.0	2.0	40.0
64-65	28.20066088156244	34.0	23.0	38.0	2.0	40.0
66-67	27.832530428263407	33.0	22.5	38.0	2.0	40.0
68-69	27.51874838424969	33.0	22.0	38.0	2.0	40.0
70-71	27.218478389538014	33.0	22.0	37.5	2.0	40.0
72-73	26.864054022980163	32.5	21.5	37.0	2.0	39.5
74-75	26.28423874689436	32.0	19.0	36.0	2.0	39.0
76-77	26.139391164873203	32.0	20.0	36.0	2.0	39.0
78-79	25.84742977779068	32.0	18.5	36.0	2.0	39.0
80-81	25.569679294539604	32.0	18.0	35.0	2.0	37.5
82-83	25.2623406700114	32.0	17.0	35.0	2.0	37.0
84-85	24.97523681797195	31.5	13.0	35.0	2.0	37.0
86-87	24.57369569831708	31.0	9.0	35.0	2.0	36.0
88-89	24.223101272281482	31.0	7.0	35.0	2.0	36.0
90-91	23.941229820695938	31.0	2.0	34.5	2.0	36.0
92-93	23.678506994554752	31.0	2.0	34.0	2.0	35.0
94-95	23.372512379482032	31.0	2.0	34.0	2.0	35.0
96-97	22.928770787052954	31.0	2.0	34.0	2.0	35.0
98-99	22.453281367779695	31.0	2.0	34.0	2.0	35.0
100-101	20.31212460167503	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.06149633350464967
1101	2	1.2871749445841445
1101	3	1.1808151582683628
1101	4	1.7130276629201937
1101	5	2.0731877416377493
1101	6	1.8332179414767644
1101	7	1.7895690229460506
1101	8	1.8735098900315101
1101	9	2.0536109605847486
1101	10-11	2.4288866200255796
1101	12-13	2.6036329486062257
1101	14-15	2.543808916962014
1101	16-17	2.7277497672091187
1101	18-19	2.8905861756208893
1101	20-21	2.5356141237097702
1101	22-23	2.362472111883818
1101	24-25	2.3423629112443685
1101	26-27	2.3187260707006843
1101	28-29	2.307315051958504
1101	30-31	2.1079567720835612
1101	32-33	2.1001808279340786
1101	34-35	2.218121084912589
1101	36-37	2.298857815972717
1101	38-39	2.136073997057835
1101	40-41	2.2506993082846343
1101	42-43	2.25543146437078
1101	44-45	2.208417781505908
1101	46-47	2.1867975154920387
1101	48-49	2.226819311402881
1101	50-51	2.3696906560082986
1101	52-53	2.3649678639105005
1101	54-55	2.2597552627968085
1101	56-57	2.291748258712861
1101	58-59	2.3264923296478734
1101	60-61	2.317747790063013
1101	62-63	2.3906757970473507
1101	64-65	2.425661159997052
1101	66-67	2.349117840565288
1101	68-69	2.3605020970402144
1101	70-71	2.4765581847915534
1101	72-73	2.3391156375917603
1101	74-75	2.2984250323816298
1101	76-77	2.239810937055722
1101	78-79	2.3746055475193018
1101	80-81	2.1185525334936877
1101	82-83	2.2173445256702315
1101	84-85	2.258145095829761
1101	86-87	2.1133670166249203
1101	88-89	2.059134248943174
1101	90-91	2.1939663148792086
1101	92-93	2.130432817239047
1101	94-95	2.198966333053381
1101	96-97	2.0878509776203735
1101	98-99	1.9517339054036533
1101	100-101	1.6863499790556133
1102	1	-7.302336279866537
1102	2	-8.542371492015814
1102	3	-8.377463031274527
1102	4	-8.281369115745953
1102	5	-6.784209917927161
1102	6	-6.764793857692403
1102	7	-8.025450383565026
1102	8	-9.754872373901811
1102	9	-10.7683931580418
1102	10-11	-8.90283753428654
1102	12-13	-11.014300797336396
1102	14-15	-12.14297501674902
1102	16-17	-12.801119028987372
1102	18-19	-13.47777561275749
1102	20-21	-12.61989650094111
1102	22-23	-12.707449459042596
1102	24-25	-12.90467736092998
1102	26-27	-13.649080795054793
1102	28-29	-13.753269501283341
1102	30-31	-14.651466543721153
1102	32-33	-14.415102495644906
1102	34-35	-14.670476278089165
1102	36-37	-14.408096658006592
1102	38-39	-14.843486989042884
1102	40-41	-15.142755248988873
1102	42-43	-15.426700382910104
1102	44-45	-14.866457200678736
1102	46-47	-15.059757268893478
1102	48-49	-15.916790189261746
1102	50-51	-15.762187949299546
1102	52-53	-15.954889706889688
1102	54-55	-16.39860025387755
1102	56-57	-16.748093192465628
1102	58-59	-16.407621057265228
1102	60-61	-16.180848288558607
1102	62-63	-16.26789495761717
1102	64-65	-16.601619157238076
1102	66-67	-16.388534329342413
1102	68-69	-16.554006531348705
1102	70-71	-16.32457296331385
1102	72-73	-16.185736077053857
1102	74-75	-15.973448384153805
1102	76-77	-16.11963200892508
1102	78-79	-16.035749861024645
1102	80-81	-15.618601422671256
1102	82-83	-15.277750395715103
1102	84-85	-15.268184890575078
1102	86-87	-15.353325598111457
1102	88-89	-15.262714726162422
1102	90-91	-15.098060948669156
1102	92-93	-15.158112596026562
1102	94-95	-14.981526683431362
1102	96-97	-14.762101562978673
1102	98-99	-14.797751059162193
1102	100-101	-13.44528425974967
1103	1	-1.6829119602932643
1103	2	-1.5612094337852263
1103	3	-0.5873095639174579
1103	4	-0.3169846798957323
1103	5	-0.10968087296766527
1103	6	-0.28443300596915577
1103	7	-0.3408883348678522
1103	8	-0.3978400523743275
1103	9	-0.3953867239485902
1103	10-11	-1.2239818119842276
1103	12-13	-2.0067666064330503
1103	14-15	-0.8489213790425225
1103	16-17	0.0801425604355046
1103	18-19	-1.6303536076332676
1103	20-21	-0.5350882974507485
1103	22-23	-0.1884783337341034
1103	24-25	-0.031697619660516096
1103	26-27	-1.1093121418677896
1103	28-29	-0.48951861671015706
1103	30-31	-0.37794192952350514
1103	32-33	-0.32847824567611994
1103	34-35	-0.22344740078804648
1103	36-37	-0.5036466879278407
1103	38-39	-0.520417762017729
1103	40-41	-1.3493517552557428
1103	42-43	-0.70040644534539
1103	44-45	-0.6309417170355154
1103	46-47	-0.9699841579878807
1103	48-49	-0.7543317225239079
1103	50-51	-0.725022218806842
1103	52-53	-0.8917436339818927
1103	54-55	-0.9000059083701828
1103	56-57	-0.8152742166781799
1103	58-59	-0.8337537792359662
1103	60-61	-0.7752816123606827
1103	62-63	-0.5388648357157635
1103	64-65	-0.6619787705981928
1103	66-67	-0.5932147517476842
1103	68-69	-0.7185695291306367
1103	70-71	-0.5112610434894549
1103	72-73	-0.8097875079717163
1103	74-75	-0.6868611875422417
1103	76-77	-0.7883356852765147
1103	78-79	-0.738598888540821
1103	80-81	-0.8304959462254793
1103	82-83	-0.9262975946782745
1103	84-85	-0.9949315025178542
1103	86-87	-0.989106771258875
1103	88-89	-0.9649408174185652
1103	90-91	-0.9499986174536232
1103	92-93	-0.9928834382808915
1103	94-95	-1.0400278329982449
1103	96-97	-1.1280363818992498
1103	98-99	-1.4311604413665933
1103	100-101	-1.4778387866169922
1104	1	0.9408858152279009
1104	2	1.4173874964688515
1104	3	1.3006505998552242
1104	4	1.4168013227549814
1104	5	1.733944171950018
1104	6	1.5867181984075067
1104	7	1.5027618221883365
1104	8	1.5347723831519993
1104	9	1.639990583012576
1104	10-11	2.092587537992827
1104	12-13	2.348563904430371
1104	14-15	2.0990337050467573
1104	16-17	2.4971413115666934
1104	18-19	2.6065932606751403
1104	20-21	2.241430000493999
1104	22-23	2.0296764365463247
1104	24-25	1.9919522970091244
1104	26-27	1.9155160828831512
1104	28-29	1.9009036708232827
1104	30-31	1.5745056076301225
1104	32-33	1.4575068718713275
1104	34-35	1.6365363195793634
1104	36-37	1.5859526235128172
1104	38-39	1.399435634482714
1104	40-41	1.5045168331645833
1104	42-43	1.5859091709247402
1104	44-45	1.5206745595521731
1104	46-47	1.5631518676051783
1104	48-49	1.6348254914678826
1104	50-51	1.6805896527337936
1104	52-53	1.6493693552469182
1104	54-55	1.6404800927677883
1104	56-57	1.6210531032602127
1104	58-59	1.5998561467033667
1104	60-61	1.5574338175103186
1104	62-63	1.6189398377823636
1104	64-65	1.5220754308378197
1104	66-67	1.6249402840506058
1104	68-69	1.588637835759556
1104	70-71	1.6142120989107553
1104	72-73	1.3734516614184606
1104	74-75	1.376839544779589
1104	76-77	1.5846794597558542
1104	78-79	1.784746929312366
1104	80-81	1.5889739860187824
1104	82-83	1.613855111968622
1104	84-85	1.6355165369708082
1104	86-87	1.5017746396469853
1104	88-89	1.4089333368001817
1104	90-91	1.4874250227276384
1104	92-93	1.5540126804209144
1104	94-95	1.65853553322345
1104	96-97	1.7109617990301835
1104	98-99	1.6226061895278043
1104	100-101	1.3270225769977984
1105	1	1.2353222903775958
1105	2	1.2215708821579767
1105	3	1.1794612701356328
1105	4	1.2556500490232416
1105	5	1.3931391279086007
1105	6	1.1484993699674781
1105	7	0.9577305538845344
1105	8	0.9855684942222247
1105	9	1.4322119938090978
1105	10-11	-0.56655255670087
1105	12-13	0.8015058296031441
1105	14-15	1.322074769850495
1105	16-17	1.854689859949417
1105	18-19	0.6746407296247483
1105	20-21	1.2066794981038882
1105	22-23	1.6862536117027105
1105	24-25	1.7811428295472034
1105	26-27	1.6746247620112555
1105	28-29	1.510687268317632
1105	30-31	1.3298762994971547
1105	32-33	1.392794340407935
1105	34-35	1.2429410067986773
1105	36-37	-0.1813412572636075
1105	38-39	1.1599065667393056
1105	40-41	1.110173541901414
1105	42-43	1.1551340057478505
1105	44-45	1.2949097089325043
1105	46-47	1.3207860712152666
1105	48-49	1.2442831003231305
1105	50-51	1.504951653658825
1105	52-53	1.3928095800020337
1105	54-55	1.350519908339571
1105	56-57	1.2249609114818334
1105	58-59	1.3510919200723635
1105	60-61	1.290113656268904
1105	62-63	1.41561100804255
1105	64-65	1.4407697991162145
1105	66-67	1.4344385032219122
1105	68-69	1.294186365485455
1105	70-71	1.4201533783572486
1105	72-73	1.3056649206819309
1105	74-75	1.1360879190366688
1105	76-77	1.3167785248805117
1105	78-79	1.285440879256246
1105	80-81	1.1433223636292205
1105	82-83	1.1627032277547293
1105	84-85	1.0962059462804774
1105	86-87	1.0226805817871636
1105	88-89	1.2392377341746759
1105	90-91	1.1239453785323654
1105	92-93	1.1131923212757329
1105	94-95	1.1711431762403741
1105	96-97	1.1700674515327627
1105	98-99	0.9729333702060075
1105	100-101	0.5935073508958943
1106	1	-2.798423214204483
1106	2	-2.3693110260081305
1106	3	-3.2865142587457328
1106	4	-3.4544855642715824
1106	5	-3.3789113001665854
1106	6	-3.5148417123711937
1106	7	-3.587263492010937
1106	8	-3.7477024425297145
1106	9	-4.223233193863358
1106	10-11	-3.490289436487508
1106	12-13	-3.7342163448598527
1106	14-15	-4.366096016445219
1106	16-17	-3.93414138376982
1106	18-19	-3.453498560411287
1106	20-21	-4.060404742364376
1106	22-23	-4.829114276782796
1106	24-25	-4.81919759255997
1106	26-27	-5.051260023377907
1106	28-29	-4.96434926458771
1106	30-31	-5.08142966798507
1106	32-33	-5.220971340809431
1106	34-35	-5.176368128447685
1106	36-37	-5.16691661752607
1106	38-39	-5.164918339981
1106	40-41	-5.411837679167284
1106	42-43	-5.5979080359390565
1106	44-45	-5.7966418498955505
1106	46-47	-5.572401225983185
1106	48-49	-5.687826837465828
1106	50-51	-5.009636260508071
1106	52-53	-5.11514588288474
1106	54-55	-4.609453856428985
1106	56-57	-4.728149376932219
1106	58-59	-4.312114280146449
1106	60-61	-3.73103724329318
1106	62-63	-4.850333594300945
1106	64-65	-4.583809477680017
1106	66-67	-4.2317109709166445
1106	68-69	-4.846951831709884
1106	70-71	-3.3245465201425404
1106	72-73	-2.778314397904559
1106	74-75	-3.4565044147690784
1106	76-77	-4.884531008318568
1106	78-79	-4.460126261921772
1106	80-81	-3.2409751679311114
1106	82-83	-3.4213758742085254
1106	84-85	-4.101872413254039
1106	86-87	-2.7597447470089875
1106	88-89	-2.5097109820058066
1106	90-91	-2.5995746115898974
1106	92-93	-2.605316087327008
1106	94-95	-2.6040341816462416
1106	96-97	-3.60567792998258
1106	98-99	-5.120787344763116
1106	100-101	-4.250110901000415
1107	1	0.9397962541600293
1107	2	1.3879661674757457
1107	3	1.3510805296115045
1107	4	-1.1434638505329637
1107	5	0.9884466722657486
1107	6	1.1830060535046663
1107	7	1.3999321317580318
1107	8	1.3415505436508361
1107	9	1.4737490262168436
1107	10-11	2.0928451997398483
1107	12-13	0.2700082096359253
1107	14-15	1.5471641650597192
1107	16-17	2.0898687786853287
1107	18-19	2.4539619962310795
1107	20-21	1.8942492169922573
1107	22-23	1.9359117046436438
1107	24-25	1.7898842228964718
1107	26-27	1.553186632747419
1107	28-29	1.6407617087256128
1107	30-31	1.4233140988552293
1107	32-33	1.4582397438669226
1107	34-35	1.5304100864858867
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2205	9	1.1666742067074054
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2213	60-61	-0.5160576049851109
2213	62-63	-0.5109410030705881
2213	64-65	-0.4875887888525732
2213	66-67	-0.2882071671268882
2213	68-69	-0.1752598762872175
2213	70-71	-0.2727873589090848
2213	72-73	-0.4760035022657654
2213	74-75	-0.4927361057554478
2213	76-77	-0.43566095654801984
2213	78-79	-0.36953124759426004
2213	80-81	-0.3682993415798599
2213	82-83	-0.37779758411367936
2213	84-85	-0.30977759418627926
2213	86-87	-0.28390550548004967
2213	88-89	-0.4446696555359253
2213	90-91	-0.43667710932171033
2213	92-93	-0.45436738043007097
2213	94-95	-0.37193829547203094
2213	96-97	-0.3139431026456485
2213	98-99	-0.26172250825016974
2213	100-101	0.03775533283958055
2214	1	-1.1739282886304672
2214	2	-0.4008059368911532
2214	3	-0.10797676124029465
2214	4	-4.800135836246987
2214	5	-5.957563103034509
2214	6	-2.1953000099603273
2214	7	-0.9368562634965656
2214	8	-2.623437673937964
2214	9	-3.5985103210888276
2214	10-11	-0.4237298186413341
2214	12-13	-0.9413091413130275
2214	14-15	-1.8563143391100532
2214	16-17	-6.571943734996548
2214	18-19	-5.0924702445731
2214	20-21	-4.236720491853863
2214	22-23	-0.4066884782741482
2214	24-25	-1.719059353742768
2214	26-27	0.08531292801875878
2214	28-29	0.3356256143978271
2214	30-31	0.18735751854217852
2214	32-33	0.1292158136087629
2214	34-35	-0.20779459307186698
2214	36-37	-1.0063027776623201
2214	38-39	0.08181995717884405
2214	40-41	0.12912766158577327
2214	42-43	0.32634422869408297
2214	44-45	0.3235904396642759
2214	46-47	0.18326698344616688
2214	48-49	0.292115503795209
2214	50-51	0.3521383454664857
2214	52-53	0.2788169738789499
2214	54-55	0.3624866378183995
2214	56-57	0.19821987189516577
2214	58-59	0.3615525800150685
2214	60-61	0.4382835617481682
2214	62-63	0.39742070808273056
2214	64-65	0.46460363013305184
2214	66-67	0.44790086025486886
2214	68-69	0.45363825562367666
2214	70-71	0.42354241731352715
2214	72-73	0.24014509789114769
2214	74-75	0.2235944027618615
2214	76-77	0.26164301919182265
2214	78-79	0.32797090646677773
2214	80-81	0.22047291422384774
2214	82-83	0.024774297703384462
2214	84-85	-0.020409025992918828
2214	86-87	0.08693483281559011
2214	88-89	0.03942587272327103
2214	90-91	0.1762204463137813
2214	92-93	0.17422553359485704
2214	94-95	0.22156818023009706
2214	96-97	0.2687493422551981
2214	98-99	0.32369664619476524
2214	100-101	0.5910866388502072
2215	1	-0.16094932875603618
2215	2	0.42980124593020363
2215	3	0.4469645019132962
2215	4	-1.8161105698607578
2215	5	-2.06533489435564
2215	6	-2.9064353165243624
2215	7	-0.771964509045354
2215	8	-0.1444652080242328
2215	9	0.07755312294978722
2215	10-11	0.7873636859776525
2215	12-13	-3.28675485022211
2215	14-15	-3.655873938069849
2215	16-17	-4.712054874975383
2215	18-19	-3.089151428163163
2215	20-21	-2.2790526146246535
2215	22-23	-1.7431737778513536
2215	24-25	0.16589582315513596
2215	26-27	0.4317547429608126
2215	28-29	0.2851938215294396
2215	30-31	0.16578532349295472
2215	32-33	0.20664257097836014
2215	34-35	0.4581062255407957
2215	36-37	0.43079652898930476
2215	38-39	0.4365651551349572
2215	40-41	0.33147913194597933
2215	42-43	0.5320230817861145
2215	44-45	0.6852657551890395
2215	46-47	0.4860007418804244
2215	48-49	0.29109370987469063
2215	50-51	0.4201294061878649
2215	52-53	0.5196346877541664
2215	54-55	0.6084594125418867
2215	56-57	0.5714191400170598
2215	58-59	0.4622248894247356
2215	60-61	0.3363023615054139
2215	62-63	0.5029421954085151
2215	64-65	0.46189605484497065
2215	66-67	0.5326191414544539
2215	68-69	0.478429434950538
2215	70-71	0.6301190925066535
2215	72-73	0.45095952268503225
2215	74-75	0.5669690868676369
2215	76-77	0.3549942643681874
2215	78-79	0.3137006430730338
2215	80-81	0.28185435841982454
2215	82-83	0.31207036848166325
2215	84-85	0.32116909242477476
2215	86-87	0.3463715338687585
2215	88-89	0.2938026965218299
2215	90-91	0.2321692714760104
2215	92-93	0.17881826942039325
2215	94-95	0.2367056785728998
2215	96-97	0.26914812188364223
2215	98-99	0.3141851066792114
2215	100-101	0.44554362067011155
2216	1	-2.984465437935377
2216	2	-1.4601055169513764
2216	3	-2.946825118138989
2216	4	-3.715272289582199
2216	5	-3.5337805695487923
2216	6	-1.7051231226711465
2216	7	-2.5906255099776416
2216	8	-3.588145857011604
2216	9	-5.629985958646351
2216	10-11	-2.480008755541707
2216	12-13	-3.2681535265783737
2216	14-15	-5.087511916327493
2216	16-17	-6.767523260401653
2216	18-19	-5.729324012046746
2216	20-21	-2.2338745749171665
2216	22-23	-3.171817527878183
2216	24-25	-4.9207553060312925
2216	26-27	-5.392200713943399
2216	28-29	-3.509877264343835
2216	30-31	-3.961052026040072
2216	32-33	-2.2367769506090163
2216	34-35	-3.015990938981325
2216	36-37	-2.576732517037989
2216	38-39	-2.2176150587662633
2216	40-41	-3.1715770836083514
2216	42-43	-2.960974639330704
2216	44-45	-3.400176827571439
2216	46-47	-3.02532848204115
2216	48-49	-3.4002922054594222
2216	50-51	-3.4961708714899693
2216	52-53	-2.9813099096309763
2216	54-55	-3.0324297562043085
2216	56-57	-2.781295125380229
2216	58-59	-3.496965109400577
2216	60-61	-3.293467922061847
2216	62-63	-4.066585718652995
2216	64-65	-3.5321154565495405
2216	66-67	-3.1558920127374464
2216	68-69	-3.2428764210328715
2216	70-71	-3.175889671234394
2216	72-73	-3.521985754750002
2216	74-75	-3.466820488818147
2216	76-77	-3.989043703251003
2216	78-79	-4.186224004536715
2216	80-81	-4.1983614548547585
2216	82-83	-4.1523381483168045
2216	84-85	-4.381931440007222
2216	86-87	-4.520111434707022
2216	88-89	-4.61855712234474
2216	90-91	-4.897524031131855
2216	92-93	-4.741235215196085
2216	94-95	-4.965291401429898
2216	96-97	-4.684525810339942
2216	98-99	-4.64221714204454
2216	100-101	-4.197101605124958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1356301.0
3	297324.0
4	283317.0
5	293807.0
6	401420.0
7	448209.0
8	463646.0
9	471979.0
10	442120.0
11	414289.0
12	408102.0
13	412171.0
14	420470.0
15	429865.0
16	438001.0
17	446852.0
18	455464.0
19	466312.0
20	484878.0
21	520660.0
22	574849.0
23	658908.0
24	771964.0
25	915715.0
26	1084780.0
27	1274555.0
28	1452700.0
29	1605672.0
30	1801841.0
31	1939187.0
32	2065480.0
33	2364849.0
34	2719221.0
35	3124727.0
36	3580965.0
37	4086619.0
38	3785266.0
39	933055.0
40	1620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.422308338175597	16.900699808414203	11.32019465775318	41.35679719565702
2	19.00760832508903	20.237881423326083	46.21547093903903	14.539039312545857
3	21.49243511823245	26.12540762244636	31.287699207940122	21.094458051381064
4	21.675497350431623	33.51839700369804	28.196571041040524	16.609534604829808
5	19.254604114463138	37.19957589294734	29.564759070264284	13.981060922325236
6	15.776821210994479	38.51428199152937	29.669024261021516	16.039872536454638
7	14.378723072380392	18.195204247411638	48.32936058032278	19.096712099885195
8	14.984029532673004	24.93033269029472	40.14596147108297	19.93967630594931
9	19.54138424531515	22.254597087496865	37.660467747386896	20.54355091980109
10-11	21.011416164053955	34.95788242564871	26.871197810821297	17.159503599476043
12-13	18.658994371707983	30.005364391163962	33.580675543273856	17.754965693854196
14-15	18.67363032655828	29.21115832188579	34.316642727316975	17.798568624238957
16-17	19.208538360875938	29.648827383746983	32.832340383228015	18.310293872149064
18-19	21.309339517798698	29.982216001955525	28.96062372317004	19.747820757075733
20-21	20.05475986719443	31.026419761858833	30.09155836987017	18.827262001076566
22-23	20.06494855771858	31.401575651978455	32.06005716056378	16.473418629739182
24-25	19.155252031548407	30.808566866476976	32.44358568113827	17.592595420836343
26-27	19.61341569888057	30.741522641046572	31.013245169186188	18.631816490886674
28-29	19.767494327235728	31.80124833059819	29.030490954687554	19.400766387478527
30-31	19.637391454124657	30.00543096283787	30.76929715206186	19.587880430975613
32-33	20.231778089560965	30.54519082574323	30.945850351315933	18.277180733379872
34-35	17.525046833016315	32.343670367789315	29.273549433036123	20.857733366158243
36-37	18.32762505868594	30.271753091814908	31.058807910557974	20.341813938941183
38-39	19.696499809431558	28.5195934417204	31.94161841689972	19.84228833194833
40-41	21.27641705793618	29.75058854650568	29.833351772222105	19.13964262333604
42-43	20.50682051109653	31.481223208136548	29.15535214889144	18.856604131875482
44-45	19.70779701951656	30.518898208279353	30.280907600452306	19.492397171751776
46-47	19.409499869853594	29.375224576365504	30.716832995134386	20.498442558646513
48-49	20.555085653265447	30.071962063899903	28.83530718040483	20.537645102429813
50-51	19.964806025358556	30.30102613260681	30.216168406011374	19.517999436023263
52-53	19.185183087874762	30.38359985624868	31.91894920386456	18.512267852012005
54-55	19.791050834567237	30.372372557465415	30.78365132453582	19.052925283431527
56-57	21.22660424490479	28.667947523405836	30.19021038252489	19.915237849164484
58-59	20.647114011358937	29.16128879308578	28.757603822277105	21.433993373278174
60-61	21.995016284434485	29.691874616349878	29.253284799047506	19.059824300168128
62-63	18.44120463547721	33.539406393139934	29.400369780881437	18.61901919050142
64-65	18.026241530039172	31.947722029753077	30.6560421111732	19.369994329034547
66-67	19.18116372434293	29.04713802879068	31.8119483001093	19.959749946757086
68-69	21.687076858558985	30.815941574081705	27.8766171865319	19.620364380827407
70-71	19.99247498590053	32.527195822316315	28.0020653945656	19.478263797217558
72-73	18.930457897595545	31.918126648479074	30.255293534191008	18.896121919734373
74-75	20.144936782940857	29.631197776773945	30.965510320134243	19.258355120150956
76-77	20.2921891033187	30.552841979303885	29.192911907415738	19.96205700996168
78-79	20.14290418297676	32.07129292792187	28.145654436782035	19.640148452319337
80-81	19.78746602535524	31.39191057568959	30.99735390137997	17.8232694975752
82-83	19.118843158043546	31.823543095203878	30.555759971882402	18.501853774870177
84-85	19.308449910697163	31.51611134266936	29.877311912244387	19.298126834389095
86-87	20.56966182618503	31.786543937429702	28.51228279002947	19.131511446355802
88-89	19.96149292834532	30.735527152835708	30.416017267518757	18.88696265130022
90-91	20.099111628232144	31.04942291471649	29.602543406237714	19.24892205081365
92-93	18.62766829024541	31.896462248236485	29.796931645663626	19.678937815854482
94-95	18.052677996539785	31.48795235501026	29.905539856007895	20.55382979244206
96-97	19.246536439041375	29.41298878542067	31.401352117617147	19.93912265792081
98-99	20.129589514688277	30.460320030101425	29.74136433240277	19.668726122807527
100-101	21.11903823978957	31.392734793640898	28.014495373617464	19.473731592952063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38233.0
1	21808.0
2	5121.0
3	4651.0
4	4330.5
5	4178.0
6	4021.5
7	3979.5
8	4253.0
9	4649.5
10	5265.5
11	6323.0
12	7681.5
13	9386.5
14	11760.0
15	14875.0
16	19106.0
17	25391.5
18	34442.0
19	46162.0
20	62287.5
21	85142.0
22	115050.0
23	153016.0
24	205261.0
25	268443.0
26	338436.0
27	432521.5
28	551038.0
29	694530.5
30	873836.0
31	1107440.0
32	1455867.0
33	1903902.0
34	2373947.5
35	2830869.5
36	3195437.0
37	3267342.0
38	2961223.5
39	2463168.5
40	2025089.5
41	1718716.5
42	1502760.5
43	1364793.0
44	1266830.5
45	1186555.5
46	1114448.5
47	1041260.0
48	971670.5
49	909440.5
50	830575.5
51	733764.0
52	647347.0
53	570303.0
54	496148.0
55	425973.5
56	360553.5
57	295874.0
58	236886.5
59	188556.5
60	145841.5
61	109191.5
62	81399.5
63	59613.5
64	42470.5
65	30399.0
66	21790.5
67	15972.5
68	12113.0
69	9596.5
70	9248.0
71	11422.5
72	11521.5
73	12861.0
74	13282.5
75	8871.5
76	5537.5
77	4280.0
78	3588.0
79	1957.0
80	896.0
81	452.5
82	265.5
83	197.5
84	155.5
85	129.0
86	108.5
87	91.0
88	80.5
89	72.0
90	71.5
91	64.5
92	54.0
93	59.0
94	59.5
95	55.0
96	54.0
97	61.5
98	79.0
99	100.5
100	172.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22477184471743758
2	0.4424752070201346
3	0.13138941374002316
4	0.11292563965570573
5	0.10435592677623684
6	0.09928077000877153
7	0.1210848952630963
8	0.11817541084278443
9	0.08916447226986952
10-11	0.11618775449484728
12-13	0.10120946564359248
14-15	0.10647397700895024
16-17	0.08856579425976639
18-19	0.14521456710590885
20-21	0.1401643552555312
22-23	0.1626000404561201
24-25	0.14471113332468577
26-27	0.1180234736205234
28-29	0.1370337681610335
30-31	0.1440716363593483
32-33	0.15336928727382898
34-35	0.1532785784844194
36-37	0.15971550095289583
38-39	0.1401462134976493
40-41	0.14585633179098154
42-43	0.16463645277836486
44-45	0.16446297221861908
46-47	0.15833219191439993
48-49	0.17277303118840306
50-51	0.1639436643992493
52-53	0.16445843677914856
54-55	0.18292447858320127
56-57	0.14661488404241904
58-59	0.13572416001393287
60-61	0.15002326680448355
62-63	0.1366754684428657
64-65	0.1313497286446565
66-67	0.12186725857175383
68-69	0.10747744299179358
70-71	0.11202989036028625
72-73	0.10032959038631967
74-75	0.12439576607654551
76-77	0.10089198488065898
78-79	0.10915555559587056
80-81	0.10706925343945052
82-83	0.09567056019027076
84-85	0.1028841766680666
86-87	0.11352771924541172
88-89	0.09672845144675984
90-91	0.09361147067067357
92-93	0.0859624520037118
94-95	0.08304276284459135
96-97	0.09081310451738843
98-99	0.08643867314811203
100-101	0.09868662743813889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.409716E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.18522424289194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.98215118925489	49.44705523590142
2	8.195776785272614	9.5374622019155
3	2.6400466742393784	4.6083512325695795
4	1.2894016824367558	3.0009650412697915
5	0.7354111109716741	2.139503020130057
6	0.4564832518250521	1.59363482223391
7	0.30801477464754184	1.2545336113093732
8	0.21620237529385222	1.0063826950654937
9	0.16591488716545238	0.8688415423460365
>10	0.8811404366603617	9.459898931203398
>50	0.07218803150302996	2.933703894226798
>100	0.04938465842341358	5.494788082021882
>500	0.0046791382089916254	1.8740496463052776
>1k	0.0027673362488850693	3.1662028729967333
>5k	2.6500992273592697E-4	1.089380332943502
>10k+	1.726579252491668E-4	2.525246837561336
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC	140529	0.31868038667342746	No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA	93593	0.2122426931802411	No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA	88330	0.20030768421367726	No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG	75841	0.17198613244027505	No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT	44660	0.10127636337578201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01665640145533182	0.0	0.0	1.1338598676195927E-5	0.0
2	0.016694952690830885	4.535439470478371E-6	0.0	3.628351576382697E-5	0.0
3	0.01672896848685947	4.535439470478371E-6	0.0	4.3086674969544524E-5	0.0
4	0.016942134141971956	4.535439470478371E-6	0.0	5.215755391050126E-5	0.0
5	0.017019236612970088	4.535439470478371E-6	0.0	6.349615258669719E-5	0.0
6	0.01714396119840824	4.535439470478371E-6	0.0	7.710247099813231E-5	0.0
7	0.01720292191152446	6.803159205717557E-6	0.0	8.617334993908905E-5	2.2677197352391856E-6
8	0.017556686190221774	9.070878940956742E-6	0.0	9.977966835052416E-5	2.2677197352391856E-6
9	0.017649662699366582	1.1338598676195927E-5	0.0	1.0204738808576335E-4	4.535439470478371E-6
10-11	0.017994356099122935	1.247245854381552E-5	0.0	1.099844071591005E-4	4.535439470478371E-6
12-13	0.018477380402728883	1.9275617749533077E-5	0.0	1.1792142623243765E-4	4.535439470478371E-6
14-15	0.01901709769971581	2.040947761715267E-5	0.0	1.235907255705356E-4	4.535439470478371E-6
16-17	0.019635051327568487	2.1543337484772262E-5	0.0	1.3492932424673154E-4	5.669299338097964E-6
18-19	0.020364123222447884	2.6078776955250635E-5	0.0	1.405986235848295E-4	6.803159205717557E-6
20-21	0.021212250403427342	3.5149655896207374E-5	0.0	1.4853564265816663E-4	6.803159205717557E-6
22-23	0.022122739877125874	4.3086674969544524E-5	0.0	1.6327582093722136E-4	6.803159205717557E-6
24-25	0.022923244943665308	4.64882545724033E-5	0.0	1.7914985908389563E-4	9.070878940956742E-6
26-27	0.023715812991131402	4.7622114440022894E-5	0.0	1.9275617749533075E-4	9.070878940956742E-6
28-29	0.02474309003119475	4.7622114440022894E-5	0.0	2.0522863603914627E-4	1.1338598676195927E-5
30-31	0.025849737261991475	5.4425273645740455E-5	0.0	2.188349544505814E-4	1.1338598676195927E-5
32-33	0.02691669939742151	5.4425273645740455E-5	0.0	2.2337039392105975E-4	1.1338598676195927E-5
34-35	0.028106118398554467	5.4425273645740455E-5	0.0	2.3130741299439691E-4	1.247245854381552E-5
36-37	0.029335222495054103	5.4425273645740455E-5	0.0	2.471814511410712E-4	1.3606318411435114E-5
38-39	0.030801303303886238	5.4425273645740455E-5	0.0	2.539846103467888E-4	1.3606318411435114E-5
40-41	0.032536108901344216	5.555913351336005E-5	0.0	2.891342662429961E-4	1.3606318411435114E-5
42-43	0.03455437946570709	6.122843285145801E-5	0.0	3.0614216425729003E-4	1.4740178279054704E-5
44-45	0.036630476883318566	6.349615258669719E-5	0.0	3.1634690306586635E-4	1.700789801429389E-5
46-47	0.03890159819816061	7.143317166003434E-5	1.1338598676195928E-6	3.2315006227158396E-4	1.8141757881913485E-5
48-49	0.04156957046666951	7.710247099813231E-5	2.2677197352391856E-6	3.3108708134492107E-4	1.8141757881913485E-5
50-51	0.04483395302554632	7.82363308657519E-5	2.2677197352391856E-6	3.458272596239758E-4	1.8141757881913485E-5
52-53	0.048147091558730766	8.390563020384986E-5	2.2677197352391856E-6	3.548981385649325E-4	1.8141757881913485E-5
54-55	0.05155660818066288	8.503949007146946E-5	2.2677197352391856E-6	3.582997181677913E-4	1.8141757881913485E-5
56-57	0.05609318151100887	9.524422888004579E-5	2.2677197352391856E-6	3.6850445697636763E-4	1.8141757881913485E-5
58-59	0.06144046464670287	9.977966835052416E-5	2.2677197352391856E-6	3.877800747259007E-4	1.8141757881913485E-5
60-61	0.06660633020357773	9.977966835052416E-5	2.2677197352391856E-6	4.183942911516297E-4	1.8141757881913485E-5
62-63	0.07315437093908089	1.0544896768862212E-4	2.2677197352391856E-6	4.354021891659236E-4	1.8141757881913485E-5
64-65	0.08023532581236524	1.0658282755624172E-4	2.2677197352391856E-6	4.388037687687824E-4	1.8141757881913485E-5
66-67	0.08873700709977694	1.0658282755624172E-4	2.2677197352391856E-6	4.422053483716412E-4	1.8141757881913485E-5
68-69	0.09939075441593063	1.0885054729148091E-4	2.2677197352391856E-6	4.4787464770973916E-4	1.8141757881913485E-5
70-71	0.11257981239608175	1.0885054729148091E-4	2.2677197352391856E-6	4.5241008718021746E-4	1.9275617749533077E-5
72-73	0.1271612502936697	1.0885054729148091E-4	4.535439470478371E-6	4.6488254572403303E-4	2.1543337484772262E-5
74-75	0.14482565317131535	1.0885054729148091E-4	4.535439470478371E-6	4.716857049297506E-4	2.2677197352391854E-5
76-77	0.16666719580127154	1.0885054729148091E-4	4.535439470478371E-6	4.7735500426784855E-4	2.2677197352391854E-5
78-79	0.1931643670476738	1.1111826702672009E-4	4.535439470478371E-6	4.898274628116641E-4	2.2677197352391854E-5
80-81	0.22558482224252085	1.1338598676195928E-4	4.535439470478371E-6	5.181739595021539E-4	2.2677197352391854E-5
82-83	0.2633151431974304	1.1338598676195928E-4	4.535439470478371E-6	5.408511568545458E-4	2.2677197352391854E-5
84-85	0.3139351831274395	1.1565370649719846E-4	4.535439470478371E-6	5.805362522212315E-4	2.2677197352391854E-5
86-87	0.3814667429829948	1.1565370649719846E-4	4.535439470478371E-6	6.066150291764821E-4	2.494491708763104E-5
88-89	0.46219303011803936	1.1905528610005724E-4	4.535439470478371E-6	6.270245067936347E-4	2.494491708763104E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	13720	0.0	56.51868	1
AGACGGC	7290	0.0	40.7088	9
CTGGAAT	186520	0.0	35.01713	1
GCGTGTT	22150	0.0	33.568924	2
AAGACGG	9000	0.0	30.864134	8
CCCGAAA	9150	0.0	26.512781	1
CGAGCTA	8670	0.0	26.048939	12-13
CCGAAAC	9675	0.0	25.468195	1
GTTTTTT	347610	0.0	25.038866	1
CATACGA	8385	0.0	24.72798	14-15
ACGGCAT	6855	0.0	24.348816	10-11
CGGCGAG	5305	0.0	23.98747	1
ACTCGTC	9830	0.0	23.899853	42-43
GAAGACG	13610	0.0	23.375605	7
TCGTCTA	10150	0.0	23.05268	44-45
CTAACGA	10360	0.0	22.706244	94-95
CTCTACG	4420	0.0	21.70095	6
TCTACGC	5000	0.0	21.462713	7
CGAAACC	11275	0.0	21.292282	2
CCCGTAT	6030	0.0	21.261482	1
>>END_MODULE