FastQCFastQC Report
Fri 27 May 2016
SRR1233073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44097160
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1600030.3628419607974754No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1026890.23286987189197672No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA974000.22087590221229664No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG814640.184737520511525No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT504020.11429761009552543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC447460.1014713872730126TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT328200.066.204151
GCGTGTT494950.042.9497572
GCGCGTT16250.042.406471
GCGGGTT43900.040.2713281
CTGGAAT1867000.032.9667781
GATAACT272550.031.3519272
CGCTTGT42300.029.8853821
CGTGTTT730600.029.256143
TCGTACC25100.028.369752
TGCCGTC119150.027.63611848-49
TATGCCG125350.027.13692346-47
ATAACTG324100.026.7053813
CTCGTAT122600.026.55368842-43
GTTTTTT5155050.026.5185011
CGAGCTA85100.026.3766312-13
TAACTGA347700.026.2717254
CCGAAAC87100.026.0266191
CGCGGGT11350.025.9605181
CGGCGAG46900.025.8395441
CGTATGC133050.025.52010744-45
Produced by FastQC (version 0.11.5)