FastQCFastQC Report
Fri 27 May 2016
SRR1233072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75016355
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTT2358020.31433412087270834No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1801100.24009431010077736No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1592980.2123510266527879No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT1372860.1830080920353968No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1259070.16783939982154558No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1119460.14922879150819846No Hit
CGGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAAC959860.12795343095515638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA912950.12170012792543707No Hit
CCGGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTT877540.11697982393306099No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT844900.1126287727522885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT62450.036.2810131
CCGGAGA471250.034.6535721
CGCGTGC97250.032.480711
CGGCGAG143250.032.230361
GGAGAAT1526750.031.3233911
GAGAATT1596300.029.7176192
GAATTGT1546150.029.5571164
CGGAGAT256850.029.4967771
AATTGTA1628700.028.808555
CGGCAAG185000.028.6540451
AGAATTG1624000.028.4590443
CGGAGAA612950.027.0454272
CGCGTGT3007300.027.0203271
ATTGTAA1852100.026.810916
GTAATAC1945550.025.5182449
TTGTAAT1958850.025.2600947
CGAGAAT200900.024.518411
CGCGAGG66250.024.4490491
GTTTTTT12832850.024.3610061
GCGTGTA155150.023.6964172
Produced by FastQC (version 0.11.5)