##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233072_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75016355 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02192353920688 33.0 31.0 34.0 30.0 34.0 2 32.181516843893576 34.0 31.0 34.0 30.0 34.0 3 32.20381403495278 34.0 31.0 34.0 30.0 34.0 4 35.640167107559414 37.0 35.0 37.0 33.0 37.0 5 35.61483289877254 37.0 35.0 37.0 33.0 37.0 6 35.64083693482574 37.0 35.0 37.0 33.0 37.0 7 35.68849650186283 37.0 35.0 37.0 35.0 37.0 8 35.67599430817453 37.0 35.0 37.0 33.0 37.0 9 37.30812955388195 39.0 37.0 39.0 34.0 39.0 10-11 37.25858642425375 39.0 37.0 39.0 34.0 39.0 12-13 37.17809560328544 39.0 37.0 39.0 34.0 39.0 14-15 38.460127528190355 40.0 38.0 41.0 34.0 41.0 16-17 38.25610036638011 40.0 38.0 41.0 33.0 41.0 18-19 38.07543282394886 40.0 38.0 41.0 33.0 41.0 20-21 37.91576956118436 40.0 38.0 41.0 33.0 41.0 22-23 37.64374762010231 40.0 38.0 41.0 32.5 41.0 24-25 37.4070956660051 40.0 37.5 41.0 32.0 41.0 26-27 37.10645985798697 40.0 37.0 41.0 31.5 41.0 28-29 37.004237036043676 40.0 37.0 41.0 31.0 41.0 30-31 36.842122681114006 40.0 37.0 41.0 30.5 41.0 32-33 36.718419190055286 40.0 37.0 41.0 30.0 41.0 34-35 36.613948651597376 40.0 37.0 41.0 30.0 41.0 36-37 36.46963128907023 40.0 37.0 41.0 30.0 41.0 38-39 36.33932567104867 40.0 37.0 41.0 30.0 41.0 40-41 36.23413604939883 40.0 37.0 41.0 29.0 41.0 42-43 36.16315664764037 40.0 36.5 41.0 29.0 41.0 44-45 36.19803588164208 40.0 37.0 41.0 29.0 41.0 46-47 36.09253566105684 40.0 36.0 41.0 28.5 41.0 48-49 35.95869535383318 40.0 36.0 41.0 28.0 41.0 50-51 35.18213298953275 39.0 35.0 40.5 26.0 40.5 52-53 35.241244485952436 39.0 35.0 40.0 26.5 41.0 54-55 35.580984040080324 39.5 35.0 41.0 26.5 41.0 56-57 35.49153499926783 39.5 35.0 41.0 26.0 41.0 58-59 35.282568121311684 39.0 35.0 41.0 26.0 41.0 60-61 35.00066640134675 39.0 35.0 41.0 25.0 41.0 62-63 34.745261436656044 39.0 35.0 41.0 23.5 41.0 64-65 34.491331676938984 38.5 35.0 40.5 23.0 41.0 66-67 34.15854563314893 38.0 34.0 40.0 22.0 41.0 68-69 33.80840299425372 37.0 34.0 40.0 21.0 41.0 70-71 33.45842875197015 37.0 34.0 39.5 20.0 41.0 72-73 33.06911180901818 36.0 34.0 39.0 18.5 41.0 74-75 32.647499928515586 36.0 33.0 39.0 17.5 40.5 76-77 32.24280551887652 35.0 33.0 38.5 14.0 40.0 78-79 31.872548646225212 35.0 33.0 37.0 11.0 39.0 80-81 31.493143755118467 35.0 32.0 37.0 7.5 39.0 82-83 31.124603154872027 35.0 32.0 36.5 6.5 39.0 84-85 30.776533737209704 35.0 32.0 36.0 2.0 37.0 86-87 30.4160160341035 35.0 31.0 36.0 2.0 37.0 88-89 30.0423324886953 35.0 31.0 35.0 2.0 37.0 90-91 29.747751653622736 35.0 31.0 35.0 2.0 36.0 92-93 29.47486969341552 34.5 31.0 35.0 2.0 36.0 94-95 29.159013044555415 34.0 30.5 35.0 2.0 36.0 96-97 28.79183551373564 34.0 30.0 35.0 2.0 35.5 98-99 28.44192014528032 34.0 30.0 35.0 2.0 35.0 100-101 26.98243206431451 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21695159028661948 1101 2 0.23840751868378618 1101 3 0.2766325123529825 1101 4 0.2650444598459458 1101 5 0.22822200815715377 1101 6 0.2569396651714655 1101 7 0.22879440532206985 1101 8 0.2227612916462789 1101 9 0.28882487380545996 1101 10-11 0.2773761529402705 1101 12-13 0.3018492153971337 1101 14-15 0.32620020534560723 1101 16-17 0.2728490564018884 1101 18-19 0.3172472475727943 1101 20-21 0.28821860087588647 1101 22-23 0.2872282586062127 1101 24-25 0.3053999640989389 1101 26-27 0.2520001714363431 1101 28-29 0.193253427827905 1101 30-31 0.1581260335234944 1101 32-33 0.12412845067097322 1101 34-35 0.0489275805154179 1101 36-37 -0.09002795785401219 1101 38-39 -0.1587479445861888 1101 40-41 -0.14411945027244855 1101 42-43 -0.15326231901220666 1101 44-45 0.014492057782305778 1101 46-47 0.021394769103089573 1101 48-49 -0.01576057757734617 1101 50-51 -0.03969450649663031 1101 52-53 -0.0034425410273470902 1101 54-55 0.003193073825599413 1101 56-57 -0.01738935368975092 1101 58-59 -0.04604711723906263 1101 60-61 -0.08786441190104455 1101 62-63 -0.08781178464148098 1101 64-65 -0.15673226873951762 1101 66-67 -0.2076068746992874 1101 68-69 -0.15276003536875749 1101 70-71 -0.22228722021314695 1101 72-73 -0.2579264440336999 1101 74-75 -0.40734093421750117 1101 76-77 -0.3886180748356516 1101 78-79 -0.42910651279558465 1101 80-81 -0.5908856999918228 1101 82-83 -0.6685116438188494 1101 84-85 -0.6994773856088763 1101 86-87 -0.6778743450331639 1101 88-89 -0.7232197703432703 1101 90-91 -0.8014640482824937 1101 92-93 -0.9036270983055736 1101 94-95 -0.9791807987624068 1101 96-97 -1.1097813460366872 1101 98-99 -1.3508932558366666 1101 100-101 -1.505019467666667 1102 1 0.2558674569946504 1102 2 0.2601024383667294 1102 3 0.2937285712479536 1102 4 0.21829995429373383 1102 5 0.23789189960401558 1102 6 0.25198404518510387 1102 7 0.22191209957711067 1102 8 0.21959120822290856 1102 9 0.2761805332329885 1102 10-11 0.30010738497177414 1102 12-13 0.2940931061841212 1102 14-15 0.3462976485635991 1102 16-17 0.30984707116817134 1102 18-19 0.3744836034891179 1102 20-21 0.3406729647323701 1102 22-23 0.28420526460379136 1102 24-25 0.3093911305704111 1102 26-27 0.29454439935686594 1102 28-29 0.27940749664536924 1102 30-31 0.2506076258095433 1102 32-33 0.17712853964161468 1102 34-35 0.1261381338694676 1102 36-37 0.03382014476324002 1102 38-39 -0.07870885338068234 1102 40-41 0.003064961744996708 1102 42-43 0.03341726480326912 1102 44-45 0.12603460073867723 1102 46-47 0.19026644310302743 1102 48-49 0.12816143197088792 1102 50-51 0.13396988369396468 1102 52-53 0.11602917994374451 1102 54-55 0.1478102755218771 1102 56-57 0.06898783587985235 1102 58-59 0.07431380610170635 1102 60-61 0.07193614688957695 1102 62-63 0.011475587399111475 1102 64-65 0.05548261341320426 1102 66-67 0.04481560300215648 1102 68-69 0.06038915400421274 1102 70-71 0.03024336755915158 1102 72-73 -0.049170606747722445 1102 74-75 -0.10651696793300403 1102 76-77 -0.10302966374185729 1102 78-79 -0.15430590244264764 1102 80-81 -0.19426598221658864 1102 82-83 -0.21825747811152496 1102 84-85 -0.27094064010736574 1102 86-87 -0.3335277257915692 1102 88-89 -0.3951472820431796 1102 90-91 -0.40529682060576633 1102 92-93 -0.47480866328277926 1102 94-95 -0.5437482736991015 1102 96-97 -0.6109854430386115 1102 98-99 -0.709661260013764 1102 100-101 -0.8631881819118803 1103 1 0.1931996559156275 1103 2 0.2328979730718146 1103 3 0.27809441211226016 1103 4 0.222998899042814 1103 5 0.24006997885177128 1103 6 0.24358554265356958 1103 7 0.2260927843082854 1103 8 0.2440168296704286 1103 9 0.28878251882970574 1103 10-11 0.27782963848376596 1103 12-13 0.2988881412785034 1103 14-15 0.33976453220198266 1103 16-17 0.28372289227315406 1103 18-19 0.2632121904778444 1103 20-21 0.25955977296009536 1103 22-23 0.2353266645870704 1103 24-25 0.17629722345655097 1103 26-27 0.19953171160218375 1103 28-29 0.14601277230557486 1103 30-31 0.1001223122390158 1103 32-33 0.10113537973767706 1103 34-35 0.11088667748601466 1103 36-37 0.06092238117386728 1103 38-39 -6.384995065644716E-4 1103 40-41 0.016958048611137144 1103 42-43 0.07740512180270542 1103 44-45 0.13048113961736618 1103 46-47 0.15397162405776754 1103 48-49 0.10382466976401616 1103 50-51 -0.054250337418764616 1103 52-53 0.09841940369928892 1103 54-55 0.16644052178281754 1103 56-57 0.18194163910170147 1103 58-59 0.13264859134590523 1103 60-61 0.15214561541732508 1103 62-63 0.16674723534375602 1103 64-65 0.13694852733972596 1103 66-67 0.03533528096224359 1103 68-69 0.07648944527186075 1103 70-71 0.01253246769466898 1103 72-73 0.026518974723288125 1103 74-75 -0.023432428458690424 1103 76-77 -0.03761707125967462 1103 78-79 -0.07520212820553596 1103 80-81 -0.11900815027041389 1103 82-83 -0.1715654123942798 1103 84-85 -0.21033687410234592 1103 86-87 -0.2778777526834837 1103 88-89 -0.27471434637396186 1103 90-91 -0.3051594791906105 1103 92-93 -0.37218172098538105 1103 94-95 -0.42216834163090056 1103 96-97 -0.40713415709052114 1103 98-99 -0.49384592545449735 1103 100-101 -0.6788546799170767 1104 1 0.1340322018657787 1104 2 0.15372401905909783 1104 3 0.1773429433626319 1104 4 0.16306560212775878 1104 5 0.15822410813819943 1104 6 0.14745927212969434 1104 7 0.15277973712350956 1104 8 0.19748238432804754 1104 9 0.22690694161060776 1104 10-11 0.22742764623584577 1104 12-13 0.21311519736271123 1104 14-15 0.18414126522321084 1104 16-17 0.20831670717370088 1104 18-19 0.25287744046453753 1104 20-21 0.1828299641637514 1104 22-23 0.23464552759227786 1104 24-25 0.24794754586485368 1104 26-27 0.21037562751305217 1104 28-29 0.16994741574695382 1104 30-31 0.1493229538399774 1104 32-33 0.10617620620535462 1104 34-35 0.09162972555203908 1104 36-37 0.06151696699427589 1104 38-39 -0.017380996933887616 1104 40-41 0.00840998768543244 1104 42-43 0.04486905445418188 1104 44-45 0.08657276078846365 1104 46-47 0.10778171782552448 1104 48-49 0.10591852173109828 1104 50-51 0.002374472298299679 1104 52-53 0.08126005210637288 1104 54-55 0.1414060145986724 1104 56-57 0.1437256731980412 1104 58-59 0.13860817219297417 1104 60-61 0.05421541424838239 1104 62-63 0.0030532442503883317 1104 64-65 0.06535780824948745 1104 66-67 -0.002487343157554278 1104 68-69 0.07457608328786591 1104 70-71 0.034091202051058644 1104 72-73 0.006215144944135886 1104 74-75 -0.02331139737857768 1104 76-77 -0.05574537431365911 1104 78-79 -0.08121294271371582 1104 80-81 -0.11367051388078764 1104 82-83 -0.13423898569671877 1104 84-85 -0.16875496946752477 1104 86-87 -0.1689596489422307 1104 88-89 -0.1363006072098294 1104 90-91 -0.1690880726023707 1104 92-93 -0.2409940926605394 1104 94-95 -0.24679014578105551 1104 96-97 -0.20081055232805411 1104 98-99 -0.2569080363320424 1104 100-101 -0.3738124028800236 1105 1 0.12322018114402056 1105 2 0.16649362105350463 1105 3 0.18887613216454469 1105 4 0.11594387546529816 1105 5 0.15782164667516696 1105 6 0.1837871155482418 1105 7 0.15884812280391714 1105 8 0.1201458479880273 1105 9 0.19073351704599162 1105 10-11 0.1938260601066446 1105 12-13 0.1564454573619969 1105 14-15 0.20818750432331257 1105 16-17 0.17711692179374694 1105 18-19 0.19774071381468872 1105 20-21 0.06431987091442437 1105 22-23 0.16311655114544266 1105 24-25 0.18483641926763283 1105 26-27 0.18436853336847747 1105 28-29 0.16108331731111747 1105 30-31 0.19128692479792875 1105 32-33 0.11240281866665214 1105 34-35 0.07844022450023402 1105 36-37 0.06218021047524758 1105 38-39 6.514257238237064E-4 1105 40-41 0.01623508256005124 1105 42-43 0.06795881547475346 1105 44-45 0.14071599330002726 1105 46-47 0.14376809035422866 1105 48-49 0.1214013407453578 1105 50-51 -0.020296624553353126 1105 52-53 0.04079796179605211 1105 54-55 0.07660152484736216 1105 56-57 0.03804951512913135 1105 58-59 0.15775217042038747 1105 60-61 0.07731345701080983 1105 62-63 0.1255760889565849 1105 64-65 0.15366786416421974 1105 66-67 0.1199259793477836 1105 68-69 0.07141607290303398 1105 70-71 0.08183632983038791 1105 72-73 0.11618623116469706 1105 74-75 0.11368142915842583 1105 76-77 0.09779033716912267 1105 78-79 0.1540358769827499 1105 80-81 0.08691631167470604 1105 82-83 0.08818643064190113 1105 84-85 0.0018253915614501182 1105 86-87 0.072113534224723 1105 88-89 0.017345188675278678 1105 90-91 -0.05094118445031981 1105 92-93 -0.02583265503357879 1105 94-95 2.0752923582278981E-4 1105 96-97 0.013625295247084779 1105 98-99 0.01716262687368797 1105 100-101 -0.015812913024102926 1106 1 0.021475665200732408 1106 2 0.06262364291492872 1106 3 0.03688447689653884 1106 4 0.09519288298007922 1106 5 0.07442969525101972 1106 6 0.06478672389103934 1106 7 0.10519757461621282 1106 8 0.130179102750148 1106 9 0.10337262182195417 1106 10-11 0.0840110968895047 1106 12-13 -0.010187603936323342 1106 14-15 0.04291858045527164 1106 16-17 0.07428743593521148 1106 18-19 0.04065725332863224 1106 20-21 0.08595911792861699 1106 22-23 0.1041617985095229 1106 24-25 0.07168490795895366 1106 26-27 0.136927917493459 1106 28-29 0.11358326194578439 1106 30-31 0.10485439587710488 1106 32-33 0.1210230135672461 1106 34-35 0.05323663040754667 1106 36-37 0.02119668714848899 1106 38-39 0.0379627963548117 1106 40-41 0.034919631353275804 1106 42-43 0.018040211713447718 1106 44-45 0.08053093308986803 1106 46-47 0.14981571574898567 1106 48-49 0.07174825338154989 1106 50-51 0.009951409463752725 1106 52-53 0.06767801296870601 1106 54-55 0.11511854355959628 1106 56-57 0.1527394382522047 1106 58-59 0.12913860220374573 1106 60-61 0.10594722063576967 1106 62-63 0.07352578673403087 1106 64-65 0.06996286368287485 1106 66-67 0.13170376496974967 1106 68-69 0.11229334824471948 1106 70-71 0.0988320850213853 1106 72-73 0.12035716990006051 1106 74-75 0.056576954258332535 1106 76-77 0.024935522408654265 1106 78-79 0.04537317216562187 1106 80-81 -0.017296661784136802 1106 82-83 0.06411287472785077 1106 84-85 -0.038528586226167505 1106 86-87 -0.025953943951090963 1106 88-89 -0.03544198179517011 1106 90-91 0.0024503593840030646 1106 92-93 -0.03595090218943753 1106 94-95 -0.005851075650873838 1106 96-97 -0.04610196808373246 1106 98-99 -0.01842716015234558 1106 100-101 -0.15252587020530584 1107 1 -0.06327718016087402 1107 2 -0.02335036242576649 1107 3 0.0013967595405048883 1107 4 0.07447764121823042 1107 5 0.06711935227887977 1107 6 0.09389204985031796 1107 7 0.08019392987392138 1107 8 0.06821675029285501 1107 9 -0.015703325210253638 1107 10-11 0.06066455091904999 1107 12-13 0.03891062649350374 1107 14-15 0.04283857042594974 1107 16-17 0.0329051965424938 1107 18-19 0.04728267991457358 1107 20-21 0.021447971445368808 1107 22-23 -0.027831332640353423 1107 24-25 0.018339302450705475 1107 26-27 0.0018613005954719597 1107 28-29 0.05506380998328808 1107 30-31 0.040841022512687175 1107 32-33 0.030067823704911234 1107 34-35 0.006071581391502434 1107 36-37 -0.07180504809874577 1107 38-39 -0.11586446763563885 1107 40-41 -0.09520167057771545 1107 42-43 -0.06724712640729535 1107 44-45 -0.044102942287402414 1107 46-47 -0.10155833280758486 1107 48-49 -0.11403055841406484 1107 50-51 -0.14599553950162658 1107 52-53 -0.13900514087848137 1107 54-55 -0.04923286758038614 1107 56-57 -0.03113723608841923 1107 58-59 -0.02060327980755261 1107 60-61 0.010947780256252315 1107 62-63 -0.03880310124245767 1107 64-65 -0.02997969026958458 1107 66-67 -0.021247490119471024 1107 68-69 0.05132101786521304 1107 70-71 -0.021408011955855955 1107 72-73 -0.016291798020297676 1107 74-75 -0.015502931996714153 1107 76-77 -0.04114850047871954 1107 78-79 -0.0381369967738685 1107 80-81 -0.005574995102026747 1107 82-83 -0.0503346376653262 1107 84-85 -0.05726145745866873 1107 86-87 -0.10322638934805184 1107 88-89 -0.08418042641206114 1107 90-91 -0.14561110992081439 1107 92-93 -0.1858565211490344 1107 94-95 -0.1712322510254154 1107 96-97 -0.22657994917804913 1107 98-99 -0.21421645250586252 1107 100-101 -0.2857151214314406 1108 1 -0.09462370272379417 1108 2 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-1.1448637869220306 2316 60-61 -1.1534042854073974 2316 62-63 -1.231774580732079 2316 64-65 -1.2367819545179088 2316 66-67 -1.2866856701261966 2316 68-69 -1.142719737513886 2316 70-71 -1.123424971445516 2316 72-73 -1.188702628585542 2316 74-75 -1.1897781425455562 2316 76-77 -1.170585767664015 2316 78-79 -1.151584678824694 2316 80-81 -1.0743757947705355 2316 82-83 -0.9996011950338861 2316 84-85 -0.9694648524677199 2316 86-87 -0.9558810982956629 2316 88-89 -0.9148861134835613 2316 90-91 -0.9765045345945467 2316 92-93 -1.076032592683891 2316 94-95 -0.9420849250301018 2316 96-97 -1.1730931745841424 2316 98-99 -1.206284904073403 2316 100-101 -1.2723729291626995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 270140.0 3 62384.0 4 48564.0 5 63337.0 6 119518.0 7 221723.0 8 363682.0 9 559256.0 10 649476.0 11 553578.0 12 396300.0 13 297503.0 14 246556.0 15 228592.0 16 237957.0 17 254403.0 18 275976.0 19 300983.0 20 351337.0 21 410267.0 22 478882.0 23 520267.0 24 547684.0 25 584257.0 26 647800.0 27 744937.0 28 876121.0 29 1046040.0 30 1268770.0 31 1559930.0 32 1956629.0 33 2518494.0 34 3380685.0 35 4912171.0 36 7482250.0 37 1.3589343E7 38 2.0230572E7 39 6745118.0 40 14873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.62377844138121 13.434775656588489 17.26165314217146 35.679792759858834 2 23.482808515556375 20.129742374179603 41.10143448052095 15.286014629743075 3 22.989154831636384 25.692324027207135 31.449945815149245 19.868575326007242 4 22.804368460717665 32.97302834828485 28.07785982136829 16.144743369629197 5 19.376054728332242 35.12088263952574 31.93533863382192 13.5677239983201 6 16.66707746597392 35.94780764807888 31.8295963593539 15.555518526593302 7 13.561572006531108 17.216092410781624 51.23254388992907 17.9897916927582 8 16.16222355778283 22.89288915730443 41.403068704151245 19.54181858076149 9 20.58945945854074 20.7867137772823 38.61115352778737 20.012673236389585 10-11 20.33492363098687 34.205350953135486 28.585055218958587 16.87467019691906 12-13 17.814490586752715 29.743647901847538 35.70056489681483 16.741296614584915 14-15 18.215592527463805 27.821531698743783 36.78321709829972 17.179658675492693 16-17 19.273700381736756 27.543484350845894 34.56198251701246 18.620832750404894 18-19 20.261858897303128 28.435948400852055 31.26433629039961 20.03785641144521 20-21 18.822307482148393 29.800892751987217 32.48622983614706 18.890569929717326 22-23 19.40526969085608 29.16147552090474 35.122552275433804 16.310702512805374 24-25 19.18690464232766 29.691325978181695 34.23970946069027 16.882059918800373 26-27 20.574871972918437 30.133672183885768 31.4529105019832 17.83854534121259 28-29 20.853655179593837 30.180062067798413 30.185507546987587 18.780775205620163 30-31 20.231594163699366 29.180404059888005 32.155316663946145 18.432685112466473 32-33 20.12486277159161 30.032222973243634 31.42865712416979 18.41425713099497 34-35 17.831874149696205 31.34656234636028 30.65313549225402 20.168428011689503 36-37 18.464172912693506 29.14944281150424 32.80293144075049 19.58345283505177 38-39 19.66662736412613 27.545251298866763 33.71374482271233 19.074376514294784 40-41 20.15750432022457 28.595480278933838 32.19787071765884 19.049144683182753 42-43 19.97305387605143 30.092406515885767 31.641214772431958 18.293324835630845 44-45 19.510626049479477 29.495870600484388 32.35868431624011 18.63481903379603 46-47 18.752865958363348 29.57456277367782 32.536191607816725 19.13637966014211 48-49 19.410044649596745 29.572099310876943 31.31210920605247 19.705746833473846 50-51 19.691649907543496 29.394857294785915 32.02065602894196 18.892836768728632 52-53 18.702628913388285 30.375617423693807 33.01667149783537 17.905082165082533 54-55 18.620806756073392 30.77299210285544 32.30998226986635 18.296218871204818 56-57 19.57681828182668 28.77543503679964 32.26782213025413 19.37992455111955 58-59 19.637601027136014 28.786267341301773 31.65674805180817 19.91938357975404 60-61 21.079495264732603 29.077645134850926 31.004127699886247 18.838731900530227 62-63 17.95968607290038 32.96887070600431 30.744085219787504 18.327358001307807 64-65 17.36634631208088 31.709447226541464 31.92163495547071 19.002571505906946 66-67 18.67867680321178 28.538685997206876 33.330679689782315 19.45195750979903 68-69 20.03800771178498 30.88509965593503 29.83489267107153 19.24199996120846 70-71 18.984789383595086 32.686235554899994 29.65165529570185 18.677319765803073 72-73 18.13598781225774 31.377465620663653 31.719603678424523 18.766942888654082 74-75 19.046091349013157 29.521644979951372 32.22152755889032 19.210736112145142 76-77 19.57456410672046 29.89334925697203 30.486502576671448 20.045584059636063 78-79 19.108535731974712 31.38584112757811 29.895034222870464 19.610588917576706 80-81 18.832135339020404 30.781606224402662 32.568616537020496 17.817641899556435 82-83 18.95286767798835 30.852488100761494 31.8300042704021 18.36463995084805 84-85 19.13719081658926 30.844577825728802 30.69340679109242 19.324824566589513 86-87 19.647962101064493 31.27274245729481 29.778075061098342 19.30122038054235 88-89 19.0184527094125 30.601800100791355 31.364493116201125 19.015254073595017 90-91 18.74026137366978 31.325806219190465 30.71413693720523 19.21979546993452 92-93 18.037627261415192 31.960135893032927 30.412137459891248 19.590099385660633 94-95 17.8276203902469 31.568440642043992 30.701697649799165 19.90224131790994 96-97 18.938005585581973 29.938806677557178 31.565072709811083 19.55811502704977 98-99 19.72522125341867 30.612730383927612 30.07295075853792 19.589097604115796 100-101 20.124240240678184 31.33548144268007 29.02192928462067 19.518349032021085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76113.0 1 71891.0 2 52721.0 3 42804.0 4 50628.5 5 59133.0 6 61881.5 7 57394.5 8 57238.5 9 61761.5 10 64923.5 11 67528.5 12 79585.5 13 91156.0 14 115021.0 15 137816.5 16 140857.0 17 159249.0 18 215850.5 19 252630.0 20 264594.0 21 290326.5 22 322821.0 23 374295.5 24 441296.0 25 525009.0 26 625057.5 27 751208.5 28 907362.0 29 1102718.0 30 1362341.5 31 1720954.0 32 2248713.5 33 2963581.0 34 3819751.0 35 4784820.5 36 5670698.5 37 5973757.5 38 5432792.5 39 4417707.5 40 3447786.5 41 2759691.5 42 2334942.0 43 2086353.0 44 1916718.0 45 1777188.0 46 1657394.0 47 1548062.5 48 1441959.5 49 1335354.5 50 1227034.0 51 1107242.5 52 986298.0 53 880608.0 54 786129.0 55 697355.5 56 611747.5 57 528609.0 58 446365.0 59 360397.5 60 277245.5 61 210220.5 62 161388.0 63 123742.5 64 94251.0 65 72655.5 66 56571.0 67 44036.0 68 33794.0 69 25608.0 70 19846.0 71 15726.5 72 12803.5 73 10809.5 74 8772.0 75 6859.5 76 5493.5 77 4484.0 78 3679.0 79 2982.5 80 2317.0 81 1796.0 82 1434.0 83 1128.0 84 839.0 85 644.0 86 531.0 87 421.0 88 311.5 89 214.5 90 169.5 91 123.5 92 86.5 93 63.0 94 43.0 95 37.5 96 25.5 97 15.0 98 9.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.065780055562551E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.0195596013695947E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.8660416785113058E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.5016355E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.40756932186026 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0990634128013 45.212180532136486 2 9.412601394319678 10.24233525720973 3 2.9974019766638387 4.892440674924563 4 1.3642318656335273 2.968981592021876 5 0.7553527758667945 2.0548454257716102 6 0.46819113217662545 1.5283884887867996 7 0.34389702294641805 1.3097420780877027 8 0.24730122391562015 1.0764046786855992 9 0.1851267235917147 0.9065065542430571 >10 0.9883276497666269 9.728945425897253 >50 0.07969856687699726 3.0024388989614685 >100 0.05066895449895602 5.2998870725432115 >500 0.0044547119940873715 1.650845460857165 >1k 0.00300691947252394 3.1164345967969362 >5k 3.1582258016299555E-4 1.1913478865880545 >10k+ 3.598468951927953E-4 5.818275376488613 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTT 235802 0.31433412087270834 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 180110 0.24009431010077736 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 159298 0.2123510266527879 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 137286 0.1830080920353968 No Hit AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA 125907 0.16783939982154558 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 111946 0.14922879150819846 No Hit CGGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAAC 95986 0.12795343095515638 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91295 0.12170012792543707 No Hit CCGGAGAATTGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTT 87754 0.11697982393306099 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84490 0.1126287727522885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0815328870617614 1.3330426411680492E-6 0.0 5.332170564672197E-6 0.0 2 0.08168485392285456 7.998255847008296E-6 0.0 1.5996511694016593E-5 1.3330426411680492E-6 3 0.08188880944695327 7.998255847008296E-6 0.0 2.2661724899856838E-5 1.3330426411680492E-6 4 0.0826259820275192 7.998255847008296E-6 0.0 2.7993895464529036E-5 1.3330426411680492E-6 5 0.08282060625312973 7.998255847008296E-6 0.0 2.9326938105697084E-5 2.6660852823360984E-6 6 0.08322051904548015 7.998255847008296E-6 0.0 4.132432187620953E-5 2.6660852823360984E-6 7 0.08341381022844951 7.998255847008296E-6 0.0 4.932257772321782E-5 2.6660852823360984E-6 8 0.08379905955174709 7.998255847008296E-6 0.0 6.131996149373027E-5 2.6660852823360984E-6 9 0.08424829492182072 1.4663469052848542E-5 1.3330426411680492E-6 6.931821734073856E-5 2.6660852823360984E-6 10-11 0.08572730573219667 1.7329554335184644E-5 2.6660852823360984E-6 7.798299450833088E-5 3.3326066029201235E-6 12-13 0.08799081213690002 1.9329118296936717E-5 2.6660852823360984E-6 9.19799422405954E-5 3.999127923504148E-6 14-15 0.09053958966681333 1.999563961752074E-5 3.3326066029201235E-6 1.0464384733169187E-4 5.332170564672197E-6 16-17 0.09339896613211879 1.999563961752074E-5 3.999127923504148E-6 1.1597470978162029E-4 5.332170564672197E-6 18-19 0.09680422355898258 2.2661724899856835E-5 3.999127923504148E-6 1.2663905091096469E-4 5.332170564672197E-6 20-21 0.10022281141225803 2.466128886160891E-5 5.332170564672197E-6 1.3263774279622092E-4 5.332170564672197E-6 22-23 0.10361473841271013 2.9993459426281112E-5 5.9986918852562215E-6 1.4996729713140554E-4 5.332170564672197E-6 24-25 0.10695134414355376 3.1993023388033186E-5 6.665213205840246E-6 1.5863207429899786E-4 6.665213205840246E-6 26-27 0.11053922841225756 3.465910867036928E-5 7.331734526424271E-6 1.7062945806951032E-4 8.664777167592322E-6 28-29 0.11435439645128052 3.599215131153733E-5 9.331298488176346E-6 1.8062727787827068E-4 9.331298488176346E-6 30-31 0.11816223275577706 3.599215131153733E-5 9.331298488176346E-6 1.872924910841109E-4 9.331298488176346E-6 32-33 0.12230932841245086 3.732519395270538E-5 9.331298488176346E-6 1.9062509768703103E-4 9.331298488176346E-6 34-35 0.12684500599902515 3.865823659387343E-5 9.331298488176346E-6 1.9862335353403932E-4 1.0664341129344394E-5 36-37 0.13158063998177463 4.065780055562551E-5 9.331298488176346E-6 2.132868225868879E-4 1.0664341129344394E-5 38-39 0.13653222687239336 4.199084319679355E-5 9.331298488176346E-6 2.2261812107506423E-4 1.0664341129344394E-5 40-41 0.14194437999553566 4.59899711202977E-5 9.99781980876037E-6 2.3061637692207252E-4 1.0664341129344394E-5 42-43 0.14780510196743096 4.932257772321782E-5 1.0664341129344394E-5 2.4727940993667314E-4 1.2663905091096469E-5 44-45 0.1536904852281879 5.1988663005553925E-5 1.0664341129344394E-5 2.572772297454335E-4 1.3330426411680492E-5 46-47 0.16015174290992945 5.1988663005553925E-5 1.0664341129344394E-5 2.679415708747779E-4 1.3330426411680492E-5 48-49 0.1669409290813983 5.465474828789002E-5 1.0664341129344394E-5 2.746067840806182E-4 1.4663469052848542E-5 50-51 0.174664578144326 5.7987354890810145E-5 1.0664341129344394E-5 2.919363384158028E-4 1.4663469052848542E-5 52-53 0.1824168876240388 6.131996149373027E-5 1.0664341129344394E-5 3.0193415822456313E-4 1.4663469052848542E-5 54-55 0.19021718663883364 6.265300413489831E-5 1.2663905091096469E-5 3.099324140715715E-4 1.4663469052848542E-5 56-57 0.1987326630306151 7.131778130249064E-5 1.3330426411680492E-5 3.159311059568277E-4 1.4663469052848542E-5 58-59 0.20817727014329074 7.598343054657881E-5 1.3996947732264516E-5 3.25262404445004E-4 1.4663469052848542E-5 60-61 0.21789848360400876 7.86495158289149E-5 1.5996511694016593E-5 3.325941389714283E-4 1.4663469052848542E-5 62-63 0.22912470220660547 8.264864375241905E-5 1.732955433518464E-5 3.4192543745960465E-4 1.4663469052848542E-5 64-65 0.24086680831133422 8.598125035533918E-5 1.866259697635269E-5 3.485906506654449E-4 1.4663469052848542E-5 66-67 0.25411258651530055 9.397950620234747E-5 1.866259697635269E-5 3.5658890651245323E-4 1.4663469052848542E-5 68-69 0.2691872992229494 1.0197776204935578E-4 1.866259697635269E-5 3.652536836800455E-4 1.4663469052848542E-5 70-71 0.28727668786359983 1.0597688997285992E-4 1.866259697635269E-5 3.705858542447177E-4 1.4663469052848542E-5 72-73 0.3073596417741171 1.0864297525519601E-4 1.866259697635269E-5 3.839162806563982E-4 1.5996511694016593E-5 74-75 0.33058457718986745 1.0930949657578004E-4 1.866259697635269E-5 3.899149725416544E-4 1.6663033014600615E-5 76-77 0.3590437045361642 1.0930949657578004E-4 1.866259697635269E-5 4.032453989533349E-4 1.732955433518464E-5 78-79 0.39323491523948345 1.0997601789636407E-4 1.866259697635269E-5 4.1257669744151123E-4 1.732955433518464E-5 80-81 0.43489316429730557 1.1197558185811614E-4 1.866259697635269E-5 4.1790886800618346E-4 1.866259697635269E-5 82-83 0.48693981465775027 1.1197558185811614E-4 1.866259697635269E-5 4.205749532885196E-4 1.866259697635269E-5 84-85 0.5555001972569849 1.1264210317870016E-4 1.999563961752074E-5 4.245740812120237E-4 1.866259697635269E-5 86-87 0.6446234291175571 1.1330862449928419E-4 1.999563961752074E-5 4.3123929441786393E-4 1.999563961752074E-5 88-89 0.7520546686119314 1.1464166714045223E-4 1.999563961752074E-5 4.359049436619521E-4 1.999563961752074E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGT 6245 0.0 36.281013 1 CCGGAGA 47125 0.0 34.653572 1 CGCGTGC 9725 0.0 32.48071 1 CGGCGAG 14325 0.0 32.23036 1 GGAGAAT 152675 0.0 31.323391 1 GAGAATT 159630 0.0 29.717619 2 GAATTGT 154615 0.0 29.557116 4 CGGAGAT 25685 0.0 29.496777 1 AATTGTA 162870 0.0 28.80855 5 CGGCAAG 18500 0.0 28.654045 1 AGAATTG 162400 0.0 28.459044 3 CGGAGAA 61295 0.0 27.045427 2 CGCGTGT 300730 0.0 27.020327 1 ATTGTAA 185210 0.0 26.81091 6 GTAATAC 194555 0.0 25.518244 9 TTGTAAT 195885 0.0 25.260094 7 CGAGAAT 20090 0.0 24.51841 1 CGCGAGG 6625 0.0 24.449049 1 GTTTTTT 1283285 0.0 24.361006 1 GCGTGTA 15515 0.0 23.696417 2 >>END_MODULE